KR20150054844A - 핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용 - Google Patents

핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용 Download PDF

Info

Publication number
KR20150054844A
KR20150054844A KR1020157006945A KR20157006945A KR20150054844A KR 20150054844 A KR20150054844 A KR 20150054844A KR 1020157006945 A KR1020157006945 A KR 1020157006945A KR 20157006945 A KR20157006945 A KR 20157006945A KR 20150054844 A KR20150054844 A KR 20150054844A
Authority
KR
South Korea
Prior art keywords
nucleic acid
biological sample
metal salt
target nucleic
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
KR1020157006945A
Other languages
English (en)
Korean (ko)
Inventor
비스와나단 스리니바산
도날드 쿨리스
후엣 루
조지 에이 로드리게즈
Original Assignee
베크만 컬터, 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 베크만 컬터, 인코포레이티드 filed Critical 베크만 컬터, 인코포레이티드
Publication of KR20150054844A publication Critical patent/KR20150054844A/ko
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/706Specific hybridization probes for hepatitis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020157006945A 2012-09-19 2013-09-18 핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용 Withdrawn KR20150054844A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261703112P 2012-09-19 2012-09-19
US61/703,112 2012-09-19
PCT/US2013/060349 WO2014047141A1 (en) 2012-09-19 2013-09-18 USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS

Publications (1)

Publication Number Publication Date
KR20150054844A true KR20150054844A (ko) 2015-05-20

Family

ID=49304332

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157006945A Withdrawn KR20150054844A (ko) 2012-09-19 2013-09-18 핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용

Country Status (10)

Country Link
US (1) US20140087366A1 (cg-RX-API-DMAC10.html)
EP (1) EP2898069B1 (cg-RX-API-DMAC10.html)
JP (1) JP2015528313A (cg-RX-API-DMAC10.html)
KR (1) KR20150054844A (cg-RX-API-DMAC10.html)
CN (1) CN105008534A (cg-RX-API-DMAC10.html)
AU (1) AU2013318156A1 (cg-RX-API-DMAC10.html)
BR (1) BR112015005718A2 (cg-RX-API-DMAC10.html)
IN (1) IN2015DN02479A (cg-RX-API-DMAC10.html)
SG (1) SG11201502138WA (cg-RX-API-DMAC10.html)
WO (1) WO2014047141A1 (cg-RX-API-DMAC10.html)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180080647A (ko) * 2017-01-04 2018-07-12 국민대학교산학협력단 필터 추출용 조성물 및 이를 이용한 식품 내 미생물 분리 방법
WO2022114917A1 (ko) * 2020-11-30 2022-06-02 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2843202T3 (es) 2012-09-28 2021-07-16 Cepheid Métodos para la extracción de ADN y ARN a partir de muestras de tejido fijado embebido en parafina
CN104749009B (zh) * 2015-03-30 2018-05-04 上海云泽生物科技有限公司 用于免疫分析的免疫抑制剂药物提取试剂
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
CN108531472B (zh) * 2017-03-05 2021-07-16 北京新羿生物科技有限公司 一种用于核酸提取的裂解液
EP3631006A4 (en) 2017-06-01 2020-06-03 Nantomics, LLC DNA STABILIZATION OF RNA
CN107254463B (zh) * 2017-06-20 2018-11-20 迈克生物股份有限公司 用于提取丙型肝炎病毒rna的试剂盒
CN109306349A (zh) * 2017-07-26 2019-02-05 天津迦美惠众科技有限公司 一种基于纳米磁珠的生物样品核酸提取纯化方法
CN107794260A (zh) * 2017-11-21 2018-03-13 苏州吉玛基因股份有限公司 一种从大体积无细胞体液中提取游离核酸的方法
US12264311B2 (en) * 2019-01-25 2025-04-01 Sekisui Chemical Co., Ltd. Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
CN111154753A (zh) * 2020-03-09 2020-05-15 重庆市大足区人民医院 一种磁珠法细菌核酸提取液
EP4159855A4 (en) * 2020-05-25 2024-07-24 Sekisui Medical Co., Ltd. METHOD FOR PURIFICATION OF NUCLEIC ACID
JPWO2022059516A1 (cg-RX-API-DMAC10.html) * 2020-09-15 2022-03-24
EP4267756A1 (en) * 2020-12-22 2023-11-01 Perkinelmer Health Sciences Inc. Detecting a target nucleic acid in a biological sample
JP2025506366A (ja) * 2022-02-05 2025-03-11 ベクトン・ディキンソン・アンド・カンパニー 非不透明溶解緩衝液組成物製剤
CN114410747A (zh) * 2022-03-30 2022-04-29 北京中海生物科技有限公司 一种病毒免提试剂及其在病毒基因组扩增中的应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
JP3856174B2 (ja) * 1997-12-11 2006-12-13 東洋紡績株式会社 植物dnaの抽出精製方法
ATE481499T1 (de) * 1999-09-24 2010-10-15 Ambion Inc Cocktail von nukleaseinhibitoren
US20030203384A1 (en) * 2002-03-08 2003-10-30 Chafin David R. Multiplex detection of biological materials in a sample
US7998699B2 (en) * 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
EP1559783A1 (de) * 2004-01-29 2005-08-03 Qiagen GmbH Verfahren zur chromatographischen Trennung eines Nukleinsäuregemisches
KR20070097430A (ko) * 2004-11-05 2007-10-04 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법
US20070185322A1 (en) * 2006-02-08 2007-08-09 Nexgen Diagnostics Llc Methods of extracting RNA
CN101400689A (zh) * 2006-02-08 2009-04-01 鲁米金公司 提取rna的方法
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
EP1911844A1 (en) * 2006-10-10 2008-04-16 Qiagen GmbH Methods and kit for isolating nucleic acids
EP2245193A4 (en) * 2008-01-24 2011-04-13 Bioventures Inc USE OF NUCLEIC ACID EFFERS FOR THE DETECTION OF SPECIFIC NUCLEOTIDE SEQUENCES IN A SAMPLE
JP5641191B2 (ja) * 2010-02-26 2014-12-17 Jsr株式会社 ウイルスの検出方法
CA2780536C (en) * 2009-11-09 2018-01-02 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample
WO2011083429A1 (en) * 2010-01-07 2011-07-14 Bigtec Private Limited A method for isolation of nucleic acids and a kit thereof
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
CN107058617A (zh) * 2010-07-29 2017-08-18 霍夫曼-拉罗奇有限公司 通用样品制备

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180080647A (ko) * 2017-01-04 2018-07-12 국민대학교산학협력단 필터 추출용 조성물 및 이를 이용한 식품 내 미생물 분리 방법
WO2022114917A1 (ko) * 2020-11-30 2022-06-02 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법

Also Published As

Publication number Publication date
BR112015005718A2 (pt) 2017-07-04
IN2015DN02479A (cg-RX-API-DMAC10.html) 2015-09-11
EP2898069A1 (en) 2015-07-29
AU2013318156A1 (en) 2015-03-19
EP2898069B1 (en) 2018-04-11
CN105008534A (zh) 2015-10-28
SG11201502138WA (en) 2015-05-28
US20140087366A1 (en) 2014-03-27
JP2015528313A (ja) 2015-09-28
WO2014047141A1 (en) 2014-03-27

Similar Documents

Publication Publication Date Title
EP2898069B1 (en) Use of divalent ions, proteases, detergents, and low ph in the extraction of nucleic acids
US10087439B1 (en) Rapid methods for the extraction of nucleic acids from biological samples
EP3775254B1 (en) Microorganism separation and detection
CA2772621C (en) Methods and compositions for direct chemical lysis
US9822404B2 (en) Control for diagnostic assay
EP1774041A4 (en) COMPOSITIONS AND METHODS FOR PREPARING NUCLEIC ACIDS FROM MICROBIAL SAMPLES
US20250122557A1 (en) Mitochondrial nucleic acid depletion and detection
JPWO2007052765A1 (ja) Rnaの抽出方法及びrnaの検出方法
CA2786644C (en) Improved recovery of nucleic acids from magnetic glass particles
JP2022552115A (ja) 抗菌剤を含有する溶液からの微生物捕捉
US20090253903A1 (en) Method for parallel isolation of viral nucleic acids
JP2004008094A (ja) 核酸抽出法
EP3245306A1 (en) Detection of particle-contained reverse transcriptase activity

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20150319

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid