KR20140083970A - 유기산으로의 글리세롤의 발효 - Google Patents

유기산으로의 글리세롤의 발효 Download PDF

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KR20140083970A
KR20140083970A KR1020147004437A KR20147004437A KR20140083970A KR 20140083970 A KR20140083970 A KR 20140083970A KR 1020147004437 A KR1020147004437 A KR 1020147004437A KR 20147004437 A KR20147004437 A KR 20147004437A KR 20140083970 A KR20140083970 A KR 20140083970A
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glycerol
gene
glpk
microorganism
strain
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KR1020147004437A
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Korean (ko)
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알. 로져스 요쿰
테론 헤르만
샤오휘 유
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미리안트 코포레이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)
    • C12Y101/05003—Glycerol-3-phosphate dehydrogenase (1.1.5.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020147004437A 2011-07-22 2011-07-22 유기산으로의 글리세롤의 발효 Ceased KR20140083970A (ko)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2011/045001 WO2013015770A1 (en) 2011-07-22 2011-07-22 Fermentation of glycerol to organic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020167026284A Division KR20160116352A (ko) 2011-07-22 2011-07-22 유기산으로의 글리세롤의 발효

Publications (1)

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KR20140083970A true KR20140083970A (ko) 2014-07-04

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KR1020177031583A Active KR101928688B1 (ko) 2011-07-22 2011-07-22 유기산으로의 글리세롤의 발효
KR1020147004437A Ceased KR20140083970A (ko) 2011-07-22 2011-07-22 유기산으로의 글리세롤의 발효
KR1020167026284A Ceased KR20160116352A (ko) 2011-07-22 2011-07-22 유기산으로의 글리세롤의 발효

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US (1) US10041094B2 (https=)
EP (1) EP2734615B1 (https=)
JP (1) JP6204912B2 (https=)
KR (3) KR101928688B1 (https=)
CN (1) CN103842495B (https=)
AU (1) AU2011373671B2 (https=)
BR (1) BR112014001371B1 (https=)
CA (1) CA2841461C (https=)
WO (1) WO2013015770A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200058090A (ko) * 2018-11-19 2020-05-27 주식회사 엘지화학 미생물 발효액의 재사용에 의한 미생물 배양 방법

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101221557B1 (ko) * 2010-08-30 2013-01-14 한국과학기술원 수크로오즈와 글리세롤을 동시에 이용하는 신규 숙신산 생성 변이 미생물 및 이를 이용한 숙신산 제조방법
RS54444B1 (sr) 2011-07-12 2016-06-30 Scientist Of Fortune S.A. Rekombinantni mikroorganizmi za proizvodnju korisnih metabolita
CN105705647B (zh) 2013-09-03 2020-03-27 Ptt全球化学公众有限公司 从1，3-丙二醇制造丙烯酸、丙烯腈和1，4-丁二醇的方法
JP7437116B2 (ja) 2016-03-02 2024-02-22 ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド 遺伝子操作された微生物からのムコン酸生成の改善
CN106434505A (zh) * 2016-09-26 2017-02-22 中国科学院青岛生物能源与过程研究所 一种利用生物柴油副产物甘油合成d‑乳酸的重组菌及其应用
KR102079741B1 (ko) * 2018-10-02 2020-02-20 이화여자대학교 산학협력단 메타노박테리움 콩고렌스를 유효성분으로 포함하는 포름산 제거용 조성물 및 이를 이용한 숙신산 제조방법
CN109370969B (zh) * 2018-11-12 2020-09-04 江南大学 一种重组克雷伯氏杆菌在制备1，3-丙二醇中的应用
CN109652434B (zh) * 2019-02-25 2023-03-31 中国科学院天津工业生物技术研究所 一株以甘油为底物生产丁二酸的重组菌及其构建方法与应用
CN112063572B (zh) * 2020-09-22 2022-07-19 浙江工业大学 一种高产o-乙酰-l-高丝氨酸的重组大肠杆菌及其应用
CN112940964B (zh) * 2021-01-29 2023-05-09 西南大学 一种喀斯特槽谷区石漠化土壤改良微生物菌剂及其制备与应用
KR102611978B1 (ko) * 2021-12-21 2023-12-11 이화여자대학교 산학협력단 숙신산 생산성이 향상된 미생물 및 이를 이용한 숙신산 생산 방법
JP2025529951A (ja) * 2022-08-29 2025-09-09 クリスチャン・ハンセン・アクティーゼルスカブ グリセロールを利用した、微生物細胞によるオリゴ糖の発酵生産

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US6849434B2 (en) 1988-08-31 2005-02-01 University Of Florida Research Foundation, Inc. Ethanol production in recombinant hosts
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US6849439B2 (en) 2001-01-10 2005-02-01 Wisconsin Alumni Research Foundation Modified barley α-glucosidase
WO2004044210A2 (en) 2002-11-06 2004-05-27 University Of Florida Materials and methods for the efficient production of acetate and other products
US7244610B2 (en) 2003-11-14 2007-07-17 Rice University Aerobic succinate production in bacteria
JP4672671B2 (ja) 2003-11-27 2011-04-20 コリア アドバンスド インスティチュート オブ サイエンス アンド テクノロジィ 新規ルーメンバクテリア変異菌株及びそれを用いたコハク酸の製造方法
US7098009B2 (en) 2004-03-04 2006-08-29 University Of Florida Research Foundation, Inc. Production of chemicals from lignocellulose, biomass or sugars
WO2006020663A2 (en) 2004-08-09 2006-02-23 Rice University Aerobic succinate production in bacteria
KR101245428B1 (ko) 2004-08-27 2013-03-19 라이스 유니버시티 숙신산 생산이 증가된 돌연변이 대장균 균주
CN101115832A (zh) * 2004-11-26 2008-01-30 协和发酵工业株式会社 工业上有用的微生物
DK2305826T3 (en) 2005-08-10 2016-06-27 Univ Florida Materials and methods for the efficient production of lactic acid
KR100679638B1 (ko) 2005-08-19 2007-02-06 한국과학기술원 포메이트 디하이드로게나제 ｄ 또는 ｅ를 코딩하는 유전자로 형질전환된 미생물 및 이를 이용한 숙신산의 제조방법
KR101390085B1 (ko) 2006-03-31 2014-04-28 라이스 유니버시티 글리세롤의 혐기성 발효
KR100885616B1 (ko) 2006-06-26 2009-02-24 씨제이제일제당 (주) 글리세롤을 이용한 아미노산의 생산 방법
WO2008006037A2 (en) 2006-07-06 2008-01-10 Integrated Genomics, Inc. Compositions and methods for enhancing glycerol utilization
RU2006145712A (ru) * 2006-12-22 2008-06-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Способ получения l-аминокислот методом ферментации с использованием бактерий, обладающих повышенной способностью к утилизации глицерина
ES2527402T3 (es) 2007-03-20 2015-01-23 University Of Florida Research Foundation, Inc. Materiales y métodos para la producción eficaz de succinato y malato
JP2010187542A (ja) * 2007-06-14 2010-09-02 Ajinomoto Co Inc 有機酸の製造方法
KR101575912B1 (ko) 2007-08-17 2015-12-08 바스프 에스이 파스테우렐라세애의 카복실산 생산 구성원
WO2009048202A1 (en) 2007-10-09 2009-04-16 Korea Advanced Institute Of Science And Technology Method for preparing succinic acid using glycerol as carbon source
JP2012506716A (ja) 2008-10-28 2012-03-22 ウィリアム マーシュ ライス ユニバーシティ グリセロールを化学物質に変換するための微好気性培養
KR101093199B1 (ko) * 2009-02-12 2011-12-12 한국과학기술원 글리세롤 대사능력 및 숙신산 생산능력이 향상된 재조합 미생물 및 이를 이용한 숙신산의 제조방법
EP2396401B1 (en) 2009-02-16 2018-12-19 Basf Se Novel microbial succinic acid producers and purification of succinic acid
CA2756705C (en) 2009-04-02 2017-04-11 University Of Florida Research Foundation, Inc. Engineered bacterial cells for succinate production
EP2295394A1 (en) * 2009-09-11 2011-03-16 Rhodia Poliamida E Especialidades Ltda Process for the purification of crude glycerol
JP2013013329A (ja) * 2009-11-06 2013-01-24 Ajinomoto Co Inc Ｌ−アミノ酸の製造法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200058090A (ko) * 2018-11-19 2020-05-27 주식회사 엘지화학 미생물 발효액의 재사용에 의한 미생물 배양 방법

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EP2734615A1 (en) 2014-05-28
KR101928688B1 (ko) 2018-12-13
CN103842495B (zh) 2018-04-13
CA2841461C (en) 2020-05-26
CN103842495A (zh) 2014-06-04
AU2011373671B2 (en) 2016-04-28
BR112014001371A2 (pt) 2017-03-01
CA2841461A1 (en) 2013-01-31
AU2011373671A1 (en) 2014-01-30
EP2734615A4 (en) 2014-12-10
KR20160116352A (ko) 2016-10-07
WO2013015770A1 (en) 2013-01-31
EP2734615B1 (en) 2019-12-25
JP2014521318A (ja) 2014-08-28
KR20170125118A (ko) 2017-11-13
US20140234923A1 (en) 2014-08-21
US10041094B2 (en) 2018-08-07
BR112014001371B1 (pt) 2021-06-01
JP6204912B2 (ja) 2017-09-27

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