KR20130104930A - Preparation method of cosmetic composition comprising saponin from sea slug and the cosmetic composition thereby - Google Patents

Abstract

PURPOSE: A method for manufacturing a cosmetic composition containing sea cucumber-derived saponin is provided to cheaply produce a cosmetic composition with high added-value, containing sea cucumber-derived saponin by an eco-friendly method. CONSTITUTION: A method for manufacturing a cosmetic composition containing sea cucumber-derived saponin comprises the steps of: steaming sea cucumber; collecting byproducts from the steamed sea cucumber and filtering the byproducts by compression; removing salts from the byproducts; and adding the byproducts containing saponin to a cosmetic composition. The cosmetic composition contains saponin and desalted byproducts of sea cucumber. [Reference numerals] (AA) Step of steaming sea cucumber; (BB) Collecting by-products from the steamed sea cucumber and filtering the by-products by compression; (CC) Process of removing salts from the by-products by using a reverse osmosis membrane; (DD) Adding the processed by-products of the previous step to a cosmetic composition

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing saponin derived from sea cucumber and a cosmetic composition comprising the same,

The present invention relates to a method for producing a cosmetic composition containing saponins derived from sea cucumber and a cosmetic composition using the same, and more particularly to a cosmetic composition for cosmetics containing a physiologically active substance which is eco- A cosmetic composition containing saponins derived from sea cucumbers containing a large amount of saponins and capable of mass-producing cosmetic compositions containing saponins derived from sea cucumbers at a low cost in a large amount, and a cosmetic composition using the same.

Sea cucumber is a medicinal herb that has been used for centuries in China and other parts of the world. It has an excellent effect on diabetes and asthma. It is a medicine that can regain energy when it is swollen due to temperature rise in the summer, It is known. When a part of the body is cut, the sea cucumber is restored to its original state within 3 months, and even when the intestines are removed, the sea cucumber has remarkable resilience that a new intestine is formed within one month. In the oriental medicine and folk therapy, It is known that it enhances immune function and improves wound healing.

According to modern science, sea cucumbers are abundant in protein and vitamin B, contain a large amount of iodine, and contain a large amount of chondroitin, which is used as a raw material for various foods and medicines.

The major components of sea cucumber are glycoprotein and chondroitin sulfate, and glycoproteins are mainly composed of polysaccharides such as fucose, glucose, mannuronic acid and N-acetylglucosamine.

Nutritional studies on sea cucumbers include studies on acidic mucopolysaccarides, which are the main factors for platelet aggregation, and studies on the structural properties of sulfated polysaccharides with structures such as chondroitin sulfate (Vieira et al., J Biol. Chem., 263, 18176, 1998) and the contents and contents of glucosaminoglycans isolated from the body wall of sea cucumber (Kariya et al., J. Biol. Chem., 265, 5081 , 1990) and revealed the structure of chondroitin sulfate extracted from sea cucumber walls (Vieira et al., J. Biol. Chem., 266, 13530, 1991).

Thus, the sea cucumber composed mainly of sticky glycoprotein can be easily degraded through contact with oxygen during circulation. To overcome such disadvantages, the sea cucumber is usually dried .

Currently, the market for dried ginseng in China is about 30 trillion won, which is about 10 trillion won.

The dried ginseng, which is consumed in such a large quantity, is produced through the process of dipping the sea cucumber, salting and drying, and a large amount of the by-product of the desserts is generated in the process. However, since the by - product of the residues contains substances that cause the eutrophication of water quality such as nitrogen compounds, it is urgent to prepare countermeasures for the reprocessing or recycling of the pollutants as they may directly or indirectly affect the marine pollution.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the problems of the prior art as described above, and its object is to recycle mature byproducts generated in the process of producing dried sea cucumber to produce environmentally- A cosmetic composition containing saponins derived from sea cucumbers containing high value added saponins, which can be mass-produced at low cost in a low cost, and a cosmetic composition using the same.

The technical problem of the present invention as described above is achieved by the following means.

(1) a step of cooking the sea cucumber;

Collecting the by-products of the human body, and filtering the filtrate under reduced pressure;

Removing salts from the mature by-products; And

A method for producing a cosmetic composition containing saponin derived from sea cucumber, which comprises the step of adding a mature by-product containing saponin to a cosmetic composition.

(2) The method according to claim 1,

Wherein the sea cucumber is cooked at a ratio of water: sea cucumber at a ratio of 3: 1 to 5: 1 for 10 to 60 minutes.

(3) The method according to claim 1,

Wherein the step of removing the salt from the mature by-products is carried out using a reverse osmosis membrane.

(4) A cosmetic composition containing a by-product of desserts of sea cucumber containing saponin and desalted

As described above, according to the present invention, cosmetics containing saponin derived from sea cucumbers derived from sea cucumbers containing environment-friendly and physiologically active substance saponin, which is highly beneficial for human body, are recycled by recycling the matured by- Can be produced.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a view showing the entire manufacturing process of saponin-containing cosmetic composition derived from sea cucumber according to the present invention.

The present invention relates to a method for preparing a sea cucumber;

Collecting the by-products of the human body, and filtering the filtrate under reduced pressure;

Removing salts from the mature by-products; And

A method for producing saponin-containing cosmetic compositions from sea cucumbers comprising the step of adding to the cosmetic composition a mature by-product containing saponin.

Hereinafter, the content of the present invention will be described in detail.

In the present invention, the method comprises firstly adding 3 to 5 times of water to the weight of the sea cucumber, using the selected sea cucumber as a raw material, and dipping it in water.

The dipping process is preferably carried out at 90 to 95 캜. If the dipping is less than 90 캜, it is difficult to obtain sufficient dipping. If the dipping temperature exceeds 95 캜, various physiologically active substances may be destroyed. At this time, the dipping time is preferably 10 to 60 minutes. If the time is exceeded, various physiologically active substances or functional oil components of sea cucumber may be destroyed.

A salting process is performed in the next step to produce dried ginseng. The salting process is a process of treating the saltwater of the immature sea cucumber, preferably sea cucumber and salt at a weight ratio of 3: 1 to 5: 1. Normally, the salting time is preferably carried out for 6 to 10 hours.

Thereafter, the sea cucumbers are prepared by removing salt from the sea cucumber which has undergone the salting treatment and drying the sea cucumber using a hot air drying method or a microwave drying method.

The present invention relates to a method for recycling matured by-products after removing the sea cucumbers after the matured process of the dried sea cucumber, and various saponin ingredients contained in the mature by-products are extracted through hot water extraction.

Thus, after the dipping process of sea cucumbers as described above, the obtained by-products of the dough are collected, filtered under reduced pressure, and desalted. Decompression filtration can be performed using equipment equipped with a 120 μm filter. Reverse osmosis membranes can be used in the process of removing salts from mature by-products that have undergone reduced pressure filtration.

Vacuum filtration can be performed continuously while maintaining a degree of vacuum of 1 atm or less by using a vacuum pump, and 10 to 14 liters of mature byproduct per minute is supplied to the reverse osmosis membrane at a pressure of 45 to 55 kgf / cm ² using a high- , Where the salt-removed mature by-products will yield 1 to 3 liters per minute.

Through the above process, the impurities contained in the by-products of the human body are removed and a cosmetic raw material containing the physiologically active substance such as saponin is obtained.

The present invention provides a cosmetic composition comprising a hot water extract of sea cucumber as a raw material for cosmetics containing saponin derived from sea cucumber obtained through the above process as an active ingredient.

The hot water extract containing saponin derived from sea cucumber of the present invention has excellent whitening effect by inhibiting melanin production, has excellent wrinkle-reducing effect through inhibition of collagenase activity and promotion of collagen synthesis, and is also excellent in hair loss prevention effect. Therefore, the hot water extract of the sea cucumber is useful as a cosmetic composition for whitening skin and improving wrinkles. In addition, the composition of the present invention does not show a clear appearance in the cumulative stimulation test of the skin and is safe for human skin.

The content of the hot water extract contained in the cosmetic preparation containing the hot water extract of sea cucumber is 0.00001 to 10.0% by weight, preferably 0.001 to 1.0% by weight, based on the total amount of the composition. If the content of the hydrothermal extract was less than 0.00001% by weight based on the total amount of the composition, significant effects such as skin whitening could not be expected. When the content of hydrothermal extract was more than 10.0% by weight, no significant increase in the effect was observed.

The ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the hot-water extract of sea cucumber as an active ingredient, and include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, , And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

Hereinafter, the content of the present invention will be described in more detail with reference to Examples to Experimental Examples. However, the following examples and the like are presented to aid the understanding of the present invention and should not be construed as limiting the scope of the present invention.

Example 1

The sea cucumber was immersed in four times the weight of water and boiled at 100 ° C for 20 minutes. The salted sea cucumbers were prepared by salting for 8 hours at a ratio of 4: 1 by weight of immersed sea cucumber and salt, and then removing the salt and drying with hot air to completely dry the water. The mature by-products obtained in the above procedure were collected, filtered under reduced pressure using a device equipped with a 120 μm filter, and desalted using a reverse osmosis membrane.

The results of analyzing the saponin content of the hot-water extract of sea cucumber obtained through the above process are shown in Table 1 below.

Saponin content (unit: mg / g) ingredient content Rg ₁ 0.016 Re 0.008 Rf 0.005 Rg ₂ + Rh ₁ 0.013 Rb ₁ 0.005 Rc 0.021 Rb ₂ + Rb ₃ 0.020 Rd 0.006 Rg ₃
(S, R) 0.0006 Rh ₂ 0.0004

[Experimental Example 1] Test for inhibiting melanin formation at the cell level

The hot-water extract of Example 1 was added to the culture of mouse melanoma cells (B-16 mouse melanoma cell), and the whitening effect at the cellular level was examined as follows (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980).

When the B-16 melanoma cells were cultured in a final concentration of 1 μg / ml, 5 μg / ml, and 50 μg / ml, the cells were incubated for 3 days and treated with trypsin , Removed from the culture container, centrifuged, and then extracted with melanin. Sodium hydroxide solution to the extraction of melanin (1N concentration) 1 ml and the reaction mixture was dissolved melanin boiled for about 10 minutes, the amount of the produced melanin by measuring the absorbance at 400 nanometers (nm) with a spectrophotometer unit cell allowance (10 ⁶ The experiment was carried out by a method showing the absorbance of the cell). The experiment was repeated three times and the average value was used to calculate the relative amount of melanin production relative to the control group as the inhibition rate (%), and the results are shown in Table 2 below.

sample Melanin production Inhibition rate (%) Control group (no addition) 0.040 0.001 - Hot water extract (final concentration 1 ㎍ / ml) 0.032 ± 0.002 20 The hot water extract (final concentration 5 / / ml) 0.021 0.002 42 The hot water extract (final concentration 50 / / ml) 0.005 0.002 78 Hydroquinone (final concentration 1 ㎍ / ml) 0.020 0.001 52 Hydroquinone (final concentration 10 ㎍ / ml) Cell death

Iterations = 3

As shown in Table 2, the hydrothermal extract of sea cucumber has comparable melanin production inhibitory activity to that of rat melanoma cells cultured as compared with hydroquinone, which is a known whitening substance. In addition, hydroquinone has a strong inhibitory effect on melanogenesis at a low concentration, but it can not be tested at a concentration of more than 10 μg / ml. However, hydrothermal extract of sea cucumber does not show cytotoxicity even at a concentration of 50 μg / ml, Melanin production inhibitory effect, it is shown that the present invention can be very useful for improvement of spots and freckles and skin whitening.

[Experimental Example 2] Wrinkle improving effect test at the cell level

The anti-wrinkle effect can usually be measured as collagen biosynthesis and collagenase degradation inhibitory effect and clinical trial in humans.

Human normal fibroblasts were inoculated into a 6-well microplate containing DMEM medium (2 x 10 ⁵ cells / well) and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. After 24 hours, the medium was removed from each well, samples were treated by concentration and then incubated again for 24 hours. After 24 hours, the cell culture medium was collected to prepare a sample.

The amount of collagen synthesis was measured by using a procollagen type I C-peptide (type I C-peptide) in a cell culture medium using a collagen measurement kit (Procollagen type I C-peptide EIA kit (MK101), Takara, : PICP) was measured and analyzed.

An antibody against collagenase was used as a method for measuring the activity of collagenase degrading collagen. The collagenase activity was measured using a Type I collagenase assay kit (Amersham Biosciences, RPN2629) and the absorbance was measured with an ELISA reader. The measured values were expressed as mean ± standard deviation. The significance was tested by t-test using SPSS / PC + program. The results are shown in Table 3 below.

sample Increase in collagen synthesis (%) Collagenase inhibition rate (%) Hot water extract (final concentration 1 ㎍ / ml) 21 10 The hot water extract (final concentration 5 / / ml) 38 25 The hot water extract (final concentration 10 ㎍ / ml) 69 38

As shown in Table 3, the hot-water extract of sea cucumbers increased collagen synthesis in a concentration-dependent manner and inhibited collagenase activity in a concentration-dependent manner. Therefore, it was found that the hot water extract of marine had wrinkle improving effect.

[Production Example 1]

Nourishing creams were prepared by mixing the respective ingredients shown in the following table in the proportions shown in Table 4 below.

Composition weight% Production Example 1 Comparative Example 1 Hot water extract
Stearic acid
Cetanol
Fiji-20 sorbitan monostearate
Sorbitan monostearate
Mineral oil
Trioctanoate
Triethanolamine
Carbomer
glycerin
Propylene glycol
antiseptic
incense
Purified water 0.01
1.0
2.0
1.0
1.0
10.0
5.0
0.5
0.2
5.0
3.0
Suitable amount
Suitable amount
Remaining amount * -
1.0
2.0
1.0
1.0
10.0
5.0
0.5
0.2
5.0
3.0
Suitable amount
Suitable amount
Remaining amount *

*: Amount to be 100% by weight in total

[Production Example 2]

Each of the ingredients shown in Table 5 below was mixed in the proportions shown in Table 5 below to prepare a soft lotion.

Composition weight% Production Example 2 Comparative Example 2 Hot water extract
ethanol
Polyoxyethylene hardened castor oil
Paraoxybenzoic Acid Methyl
glycerin
1,3-butylene glycol
incense
Pigment
Purified water 0.01
10.0
1.0
2
5.0
6.0
Suitable amount
Suitable amount
Remaining amount * -
10.0
1.0
2
5.0
6.0
Suitable amount
Suitable amount
Remaining amount *

*: Amount to be 100% by weight in total

[Production Example 3]

Each component shown in the following Table 6 was mixed as shown in the following Table 6 to prepare an essence.

Composition weight% Production Example 3 Comparative Example 3 Hot water extract
Propylene glycol
glycerin
Sodium hyaluronate aqueous solution (1%)
ethanol
Polyoxyethylene hardened castor oil
Paraoxybenzoic Acid Methyl
Carbomer
Triethanolamine
incense
Purified water 0.01
10.0
10.0
5.0
5.0
1.0
0.1
0.3
0.4
Suitable amount
Remaining amount * -
10.0
10.0
15.0
5.0
1.0
0.1
0.3
0.4
Suitable amount
Remaining amount *

*: Amount to be 100% by weight in total

[Production Example 4]

Nutritional lotion was prepared by mixing the respective ingredients shown in the following Table 7 as shown in Table 7 below.

Composition weight% Production Example 4 Comparative Example 4 Hot water extract
Polyoxyethylene hardened castor oil
Paraoxybenzoic Acid Methyl
glycerin
1,3-butylene glycol
Carbomer
Triethanolamine
Propylene glycol
ethanol
Carboxyvinyl polymer
Pigment
incense
Purified water 0.01
1.0
Suitable amount
6.0
5.0
0.2
0.3
5.0
2
One
Suitable amount
Suitable amount
Remaining amount * -
1.0
Suitable amount
6.0
5.0
0.2
0.3
5.0
3.2
0.1
Suitable amount
Suitable amount
Remaining amount *

*: Amount to be 100% by weight in total

[Experimental Example 3] Clinical whitening effect test

Twenty healthy men and women were selected as the subjects. Two 7-mm-diameter holes each having a diameter of 7 mm were covered with aluminum foil having a diameter of 2 mm. A 10 m distance from the arm was measured with a daylight irradiator (ORIEL Solar Simulator 1000 W) / Cm < 2 > The samples containing the hot-water extracts prepared according to Production Examples 1-4 and Comparative Examples 1-4 and the base materials not containing the hot-water extracts were mixed in the same row . The degree of pigmentation of the preparation examples and the comparative examples was visually determined for each of the above. The results are shown in Table 8 below.

Experimental material Distinct effect (n.) Effective (persons) No difference (persons) Production Example 1 6 7 7 Production Example 2 5 7 8 Production Example 3 3 10 7 Production Example 4 5 12 3

As shown in Table 8, whitening ointments and cosmetic compositions containing the hot-water extract of sea cucumber prepared according to Preparation Example 1-4 exhibited whitening effect for at least 10 persons among 20 subjects, It does not show any side effects, so it can be understood that the hydrothermal extract of sea cucumber is a skin whitening agent which is safe and has excellent effect on improvement of spots and freckles.

[Experimental Example 4] Clinical wrinkle improvement test

Evaluation of the wrinkle improvement effect was performed as follows. Twenty 20- to 40-year-old women were randomly divided into two groups, and the creams of Preparation Example 1 and Comparative Example 1 were washed twice daily in the morning and evening, and then a proper amount of cream was applied continuously for 2 months around the eyes. Wrinkle improvement effect of each subject was evaluated through visual observation. The experimental results are shown in Table 9 below.

Experimental material Distinct effect (n.) Effective (persons) No difference (persons) Production Example 1 12 6 2 Comparative Example 1 - 5 15

As shown in Table 9, the nutritional cream prepared according to Preparation Example 1 exhibited wrinkle-improving effect on 18 of 20 subjects and showed no side effects in the skin. Thus, the hot-water extract of sea cucumber was safe and superior Which is an effective skin wrinkle remedy.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It can be understood that

Claims (4)

Ripening sea cucumber;
Collecting the by-products of the human body, and filtering the filtrate under reduced pressure;
Removing salts from the mature by-products; And
A method for producing a cosmetic composition containing saponin derived from sea cucumber, which comprises the step of adding a mature by-product containing saponin to a cosmetic composition. The method of claim 1,
Wherein the sea cucumber is cooked at a ratio of water: sea cucumber at a ratio of 3: 1 to 5: 1 for 10 to 60 minutes. The method of claim 1,
Wherein the step of removing the salt from the mature by-products is carried out using a reverse osmosis membrane. Cosmetic composition containing saponin and containing by-products of desalted sea cucumber

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