CN105769727A - Whitening skin-care product containing sea cucumber decoction extract and preparation method of whitening skin-care product - Google Patents
Whitening skin-care product containing sea cucumber decoction extract and preparation method of whitening skin-care product Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/678—Tocopherol, i.e. vitamin E
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- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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Abstract
The invention relates to a whitening skin-care product containing a sea cucumber decoction extract and a preparation method of the whitening skin-care product. The whitening skin-care product is prepared from the following components in parts by mass (100 parts): 0.1 to 1 part of the sea cucumber decoction extract, 5 to 10 parts of natural oil, 0 to 5 parts of a natural thickening agent, 0 to 5 parts of high alcohol, 1 to 25 parts of an emulsifier, 0.1 to 0.5 part of a preservative, 0.05 to 0.5 part of an essence, 0 to 5 parts of a functional additive and the balance of water, wherein the sea cucumber decoction extract consists of 30 to 90 weight percent of sea cucumber glycosides, 5 to 60 weight percent of sea cucumber polypeptide and 5 to 10 weight percent of impurities. The sea cucumber decoction extract is applied to the formula of the whitening skin-care product, so that new application of the sea cucumber decoction extract to the field of skin-care products is developed, and the sea cucumber processing additional value is increased; meanwhile, by the adoption of the natural oil and the natural thickener, the risk of excessively introducing harmful compound substances into the skin care product is avoided.
Description
Technical field
The present invention relates to Skin whitening care cosmetics and preparation method thereof, particularly to a kind of Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract and preparation method thereof.
Background technology
Stichopus japonicus water cooking liquid is a large amount of waste liquids produced in Holothurian machining production process, rich in various active material, such as selenka, Stichopus japonicus polysaccharide etc., there is physiology and pharmacological activity widely, as antifungal, antitumor, antiinflammatory, antiviral, radioprotective, raising immunity etc. act on.
Past Stichopus japonicus water cooking liquid is taken as discharging of waste liquid, has both caused environmental pollution, is again a kind of wasting of resources.People come to realise economic worth potential in Stichopus japonicus water cooking liquid in recent years, its application in the field such as food, health product of comprehensive exploitation.Chinese patent 201010545951.9 discloses a kind of soy sauce utilizing Stichopus japonicus cooking waste liquor to purify concentrated solution brew and brewing method, utilize in Stichopus japonicus cooking waste liquor extract rich in the concentrated solution having the immunomodulator such as Stichopus japonicus polysaccharide, selenka, coordinate the raw material such as Semen Glycines, Semen arachidis hypogaeae, use the brewing method brew sea cucumber sog sauce rich in Stichopus japonicus polysaccharide and saponin isoreactivity composition of multi-cultur es and science.Chinese patent 201210144010.3 discloses one and utilizes Stichopus japonicus water cooking liquid to produce sea cucumber peptide and sea cucumber wine, main technique is that Stichopus japonicus water cooking liquid is mixed in proportion with trypsin, Multiprotein-zinc, edible ethanol, acquisition sea cucumber peptide and sea cucumber wine afterwards extracted, centrifugal, dry.Chinese patent 201510021389.2 discloses a kind of method preparing nutrient with Stichopus japonicus water cooking liquid, utilize Stichopus japonicus water cooking liquid for raw material, after removing heavy metal and impurity, remove insoluble matter through concentration, add Apostichopus enzymolysis liquid simultaneously, make the preparation of granule, dry cream.
The selenka contained in Stichopus japonicus water cooking liquid extract is similar to ginsenoside's chemical constitution, and ginsenoside has been widely used in the numerous areas such as food, medicine, cosmetics as a kind of anti-oxidation active substance.Chinese patent 201310536432.X discloses a kind of skin-care ginseng skin care item, the component such as including lanoline, vaseline, stearic acid, sucrose dilaurate, whitening agent, ginsenoside, triethanolamine, water, there is significant moist skin effect, especially also there is the effect of skin whitening.At present, the application in the field such as food, health product of the Stichopus japonicus water cooking liquid extract obtains extensive exploitation, but its application in cosmetic field rarely has research at present.
Summary of the invention
It is an object of the invention to by the extraction of part nutrient substance in Stichopus japonicus water cooking liquid and research, it is provided that a kind of key technology that Stichopus japonicus water cooking liquid is recycled, be effectively facilitated the development in pluralism of Holothurian machining industry；Effect according to Stichopus japonicus water cooking liquid extract, a kind of Skin whitening care cosmetics of exploitation and preparation method thereof, it is achieved Stichopus japonicus water cooking liquid extract value added applications.
For reaching above-mentioned purpose, the invention provides a kind of Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract, it is characterized in that, composition includes: in 100 parts of mass fractions, wherein, Stichopus japonicus water cooking liquid extract account for 0.1～1 part, natural oil lipid account for 5～20 parts, natural thickener account for 0～5 part, higher alcohol account for 0～5 part, emulsifying agent account for 1～25 part, preservative account for 0.1～0.5 part, essence account for 0.05～0.5 part, functional additive account for 0～5 part, water surplus；
Consisting of of described Stichopus japonicus water cooking liquid extract: selenka 30～90wt%, sea cucumber polypeptide 5～60wt%, impurity 5～10wt%；
Described natural oil lipid is Camellia oil, olive oil, Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, Semen Tritici aestivi germ oil, glycerol, cocoa butter, Oleum Cocois, Petiolus Trachycarpi oil, one or more mixing in Cera Flava, lanoline；It is preferably one or more mixing in Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, glycerol, Petiolus Trachycarpi oil, Cera Flava；
Described natural thickener is one or more mixing of natural plant starch, gelatin, guar gum, carrageenan, arabic gum, xanthan gum, alginate jelly；It is preferably one or more mixing in natural plant starch, gelatin, guar gum, xanthan gum；
Described higher alcohol is one or more mixing of lauryl alcohol, hexadecanol, octadecanol, hexadecanol, lanonol, cholesterol, plant sterol, octyldodecanol；It is preferably one or more mixing in hexadecanol, octadecanol；
Described emulsifying agent is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene octadecanol, polyoxyethylene ether, one or more mixing of Vinlub 73；It is preferably stearic acid, sodium hydroxide, triethanolamine, siloxanes, one or more mixing of polyoxyethylene laurel ether；
Described preservative is one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate, benzyl alcohol, chloroxylenol, benzyl chlorophenol；It is preferably one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate；
Described functional additive is one or more mixing of vitamin E, carbamide, aminoacid, hyaluronic acid, sheep placental extract；It is preferably one or more mixing in vitamin E, carbamide, aminoacid；
Described essence is the essence meeting cosmetics odor type, as: bouquet type composition, it is preferred to rose essence, jasmin essence, lavandula angustifolia essence；Milk flavoured type essence.
Under optimal way, the preparation method of described Stichopus japonicus water cooking liquid extract is:
After S1, the remove impurity by centrifugation of Stichopus japonicus water cooking liquid, pass sequentially through microfiltration systems and ultrafiltration system, collect effluent；
S2, with the NAK-9 absorption with macroporous adsorbent resin step S1 effluent obtained；Wash described adsorbent resin with water colourless to eluent, discard eluent；
S3,2～4 times of column volumes of alcoholic solution eluting macroporous adsorbent resin with 10～20%, discard eluent；
S4,3～6 times of column volumes of alcoholic solution eluting macroporous adsorbent resin with 40～100%, collect eluent；
S5, by step S4 collect eluent organic solvent is evaporated off after, in-70 DEG C～-40 DEG C lyophilization 24h～72h, prepare Stichopus japonicus water cooking liquid extract.
The preparation method that present invention also offers the above-mentioned Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract, concretely comprises the following steps:
S1, weigh Stichopus japonicus water cooking liquid extract, natural oil lipid, natural thickener, higher alcohol, emulsifying agent, preservative, essence, functional additive and water respectively according to formula, standby；
Described formula is: in 100 parts of mass fractions, wherein, Stichopus japonicus water cooking liquid extract account for 0.1～1 part, natural oil lipid account for 5～20 parts, natural thickener account for 0～5 part, higher alcohol account for 0～5 part, emulsifying agent account for 1～25 part, preservative account for 0.1～0.5 part, essence account for 0.05～0.5 part, functional additive account for 0～5 part, water surplus；
Consisting of of described Stichopus japonicus water cooking liquid extract: selenka 30～90wt%, sea cucumber polypeptide 5～60wt%, impurity 5～10wt%；
Described natural oil lipid is Camellia oil, olive oil, Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, Semen Tritici aestivi germ oil, glycerol, cocoa butter, Oleum Cocois, Petiolus Trachycarpi oil, one or more mixing in Cera Flava, lanoline；It is preferably one or more mixing in Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, glycerol, Petiolus Trachycarpi oil, Cera Flava；
Described natural thickener is one or more mixing of natural plant starch, gelatin, guar gum, carrageenan, arabic gum, xanthan gum, alginate jelly；It is preferably one or more mixing in natural plant starch, gelatin, guar gum, xanthan gum；
Described higher alcohol is one or more mixing of lauryl alcohol, hexadecanol, octadecanol, hexadecanol, lanonol, cholesterol, plant sterol, octyldodecanol；It is preferably one or more mixing in hexadecanol, octadecanol；
Described emulsifying agent is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene octadecanol, polyoxyethylene ether, one or more mixing of Vinlub 73；It is preferably stearic acid, sodium hydroxide, triethanolamine, siloxanes, one or more mixing of polyoxyethylene laurel ether；
Described preservative is one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate, benzyl alcohol, chloroxylenol, benzyl chlorophenol；It is preferably one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate；
Described functional additive is one or more mixing of vitamin E, carbamide, aminoacid, hyaluronic acid, sheep placental extract；It is preferably one or more mixing in vitamin E, carbamide, aminoacid；
Described essence is the essence meeting cosmetics odor type, as: bouquet type composition, it is preferred to rose essence, jasmin essence, lavandula angustifolia essence；Milk flavoured type essence；
After S2, the natural oil lipid that step S1 is weighed, natural thickener, higher alcohol and emulsifying agent mix homogeneously, process 10min～15min, 90 DEG C～100 DEG C agitating heating 20min～30min with the frequency ultrasonic wave of 24kHz, obtain oil phase；After functional additive and water mix homogeneously, process 10min～20min with the frequency ultrasonic wave of 24kHz, then add the step S1 Stichopus japonicus water cooking liquid extract weighed, 90 DEG C～100 DEG C agitating heating 20min～30min, obtain aqueous phase；
S3, the aqueous phase obtained by step S2 join in described oil phase, stirring while adding；Reduce mixed solution temperature to 45 DEG C～55 DEG C, add the step S1 preservative weighed, stir to uniform dissolution；Continue to reduce solution temperature to 30 DEG C～40 DEG C, add the step S1 essence weighed, stir to uniform dissolution；Finally reduce solution temperature to room temperature, with sodium hydrate regulator solution pH value to 7.5～8.5, obtain mixed liquor；
S4, the mixed liquor 100 DEG C obtained by step S3 sterilization 10min～15min, obtains the Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract.
Under optimal way, described in step S1, the preparation method of Stichopus japonicus water cooking liquid extract is:
After S1, the remove impurity by centrifugation of Stichopus japonicus water cooking liquid, pass sequentially through microfiltration systems and ultrafiltration system, collect effluent；
S2, with the NAK-9 absorption with macroporous adsorbent resin step S1 effluent obtained；Wash described adsorbent resin with water colourless to eluent, discard eluent；
S3, with 2～4 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 10～20v%, discard eluent；
S4, with 3～6 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 40～100v%, collect eluent；
S5, by step S4 collect eluent organic solvent is evaporated off after, in-70 DEG C～-40 DEG C lyophilization 24h～72h, prepare Stichopus japonicus water cooking liquid extract.
Advantages of the present invention and good effect be:
1, the present invention relates to the recycling technique of Stichopus japonicus water cooking liquid, simple to operate, operating cost is low, environmental protection, be suitable to promote, and promote the further genralrlization of Holothurian machining industry.
2, Stichopus japonicus water cooking liquid extract is applied in the formula of Skin whitening care cosmetics by the present invention, starting the Stichopus japonicus water cooking liquid extract new application in skin care field, thus improving Holothurian machining added value, the pollution to environment can also be reduced simultaneously, and start good economic benefit, there is good prospect.
3, the present invention is with natural oil lipid and natural thickener, substitute oils and the thickening agent of the chemosynthesis commonly used in skin care item, the zest of skin is little, use safety, environmental protection, finally avoid the impact that product quality and consumer health are caused by the harmful chemicals synthetic of excessive use in skin care item.
Accompanying drawing explanation
Fig. 1 is the ginsenoside Re's standard curve in embodiment 1；
Fig. 2 is Stichopus japonicus water cooking liquid extract solution DPPH clearance rate in skin care item in embodiment 1；
Fig. 3 is the Stichopus japonicus water cooking liquid extract solution DPPH clearance rate being not added in skin care item in embodiment 1
Detailed description of the invention
It is below specific embodiment of the invention example, is used for further illustrating advantages of the present invention and characteristic, but the present invention is not by the restriction of following embodiment.
Embodiment 1
The mass fraction respectively Stichopus japonicus water cooking liquid extract 0.14 part of the present embodiment each component of Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract, stearic acid 20 parts, triethanolamine 1.2 parts, sodium hydroxide 0.36 part, hexadecanol 0.5 part, glycerol 8 parts, methyl parahydroxybenzoate 0.1 part, rose essence 0.05 part, deionized water are to 100 parts.
The preparation method of described Stichopus japonicus water cooking liquid reutilization technology and Stichopus japonicus water cooking liquid extract Skin whitening care cosmetics, comprises the following steps:
(1) described Stichopus japonicus water cooking liquid extract extracts separating step:
1. 100L Stichopus japonicus water cooking liquid adopts the centrifugal 25min remove impurity of disk centrifugal separator 7000r/min, then passes sequentially through microfiltration systems and ultrafiltration system, and wherein micro-filtration membrane material is ceramic membrane (Al2O3, 0.5 μm), ultrafiltration membrane material is ceramic membrane (Al2O3, 0.05 μm), the molecular weight cut coverage of ultrafilter membrane is 10000Da.Collect effluent.
2. will 1. in gained effluent with 5LNAK-9 macroporous adsorbent resin circulation absorption 4h (absorption 1h, in stop 0.5h).NAK-9 macroporous adsorbent resin after absorption is transferred in glass chromatography column, then washes with water to eluent colourless, and eluent discards.
3. with 3 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 10v%, eluent discards.
4. with 3 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 60v%, collect eluent, decompression and solvent recovery, lyophilizing, obtain Stichopus japonicus water cooking liquid extract.
5. vanillin-ice acetic acid method is adopted to measure saponin mass content in Stichopus japonicus water cooking liquid extract.
Currently without commodity selenka standard substance, available ginsenoside Re replaces selenka to be standard substance, with ginsenoside's mass (μ g) be abscissa, absorbance under wavelength 537nm be that vertical coordinate draws its standard curve, as shown in Figure 1, obtaining computing formula is: y=0.0045x-0.0035, R2=0.9991.
In final mensuration Stichopus japonicus water cooking liquid extract, saponin mass content is 34.08%, and sea cucumber polypeptide mass content is 57.65%, impurity 8.27%
(2) according to formula precise Stichopus japonicus water cooking liquid extract 0.014g, stearic acid 2g, triethanolamine 0.12g, sodium hydroxide 0.036g, hexadecanol 0.05g, glycerol 0.8g, methyl parahydroxybenzoate 0.01g, rose essence 0.005g, standby.
(3) stearic acid, hexadecanol are placed in beaker and are mixed and stirred for putting into, after uniformly, the ultrasonic Treatment 15min that frequency is 24kHz, as oil phase；Glycerol, triethanolamine and water are placed in beaker and are mixed and stirred for putting into, after uniformly, the ultrasonic Treatment 20min that frequency is 24kHz, as aqueous phase, and backward aqueous phase adds the Stichopus japonicus water cooking liquid extract of precise.
(4) by oil phase described in (3) with aqueous phase respectively at 90 DEG C of agitating heating 25min until observing that oil phase is all thoroughly mixed into transparence liquid with aqueous phase；Oil phase carries out churned mechanically while, aqueous phase is joined in oil phase；Reduce mixture temperature to 50 DEG C, and be added thereto to the methyl parahydroxybenzoate of a small amount of precise, continuously stirred make its uniform dissolution in the mixture；Continuing to reduce mixture temperature to 40 DEG C, drip the rose essence of weighing wherein, continued mechanical stirring makes rose essence uniform dissolution in the mixture；Finally reduce mixture temperature to room temperature, and to regulate its pH value with sodium hydroxide be 7.8, standby.
(5) being placed in hermetic container by mixture and carry out 100 DEG C of high temperature sterilizations, the time is 15min, namely prepares Stichopus japonicus water cooking liquid extract Skin whitening care cosmetics.
(6) the Stichopus japonicus water cooking liquid extract solution scavenging action to DPPH free radical in the Skin whitening care cosmetics that inspection (5) prepares.Specifically comprise the following steps that
1. ready-made skin care item 3.044g in (5) is accurately weighed, skin care item then contain the Stichopus japonicus water cooking liquid extract of 4.26mg, add 7mL deionized water, it is diluted to 0.5g/L by 2g/L by original Stichopus japonicus water cooking liquid extract quality concentration, this mixture is moved in centrifuge tube, centrifugal half an hour, take supernatant and continue vacuum filtration, obtain the Stichopus japonicus water cooking liquid extract solution of clarification.Repeating above-mentioned experimentation, the Stichopus japonicus water cooking liquid extract quality content respectively obtaining clear liquor is 0.50g/L, 0.67g/L, 1.0g/L, 1.6g/L, 2.0g/L.
2. preparing the DPPH free radical alcoholic solution of 0.04g/L, cold preservation is kept in Dark Place.Accurately pipette above-mentioned solution and each 2mL of ethanol successively in same centrifuge tube, after lucifuge stands 30min after mix homogeneously, then measure absorbance A c, parallel survey twice at 517nm place；Accurately pipette successively above-mentioned solution and 1. in the Stichopus japonicus each 2mL of water cooking liquid extract solution in same centrifuge tube, after mix homogeneously, lucifuge stands after 30min, in 517nm place mensuration absorbance A i, parallel survey twice；Accurately pipette successively ethanol and 1. in the Stichopus japonicus each 2mL of water cooking liquid extract solution in same centrifuge tube, after mix homogeneously, lucifuge stands after 30min, in 517nm place mensuration absorbance A j, parallel survey twice.
According to formula: clearance rate=[1-(Ai-Aj)/Ac] × 100% calculates the Stichopus japonicus water cooking liquid extract solution clearance rate to DPPH free radical in Skin whitening care cosmetics, and parallel assay 3 times.By the Stichopus japonicus water cooking liquid extract solution in 1., make blank with ethanol, carry out above-mentioned experiment respectively, and parallel survey twice.
Finally calculate the Stichopus japonicus water cooking liquid extract solution of the different quality concentration clearance rate to DPPH free radical in Skin whitening care cosmetics, as shown in Figure 2.
(7) scavenging action of DPPH free radical is tested by the Stichopus japonicus water cooking liquid extract solution being not added in skin care item, specifically comprises the following steps that and prepares 0.50g/L, 0.67g/L respectively, 1.01g/L, 2.08g/L, 3.33g/L, the Stichopus japonicus water cooking liquid solution of extract of 5.07g/L, 10.01g/L.Other step same (6), finally the Stichopus japonicus water cooking liquid extract solution of the calculating different quality concentration clearance rate to DPPH free radical, as shown in Figure 3.
By Fig. 2, Fig. 3 it can be seen that Stichopus japonicus water cooking liquid extract solution has good oxidation resistance, it is possible to suppress the catalysis activity of tryrosinase, and then suppress melanin to deposit at epidermis, be finally reached white-skinned face function.Stichopus japonicus water cooking liquid extract is added in Skin whitening care cosmetics, its solution D PPH free radical scavenging activity decreases, but still there is stronger scavenging ability of DPPH free radical, illustrate that the Nature comparison of Stichopus japonicus water cooking liquid extract is stable, it is not affected or less affected by the impact of other compositions of skin care item, still there is good oxidation resistance.
Result: utilize ultrafiltration system, microfiltration systems and macroporous resin extraction selenka in Stichopus japonicus water cooking liquid, and adopt vanillin-ice acetic acid method to measure selenka content, a kind of key technology is provided for Stichopus japonicus water cooking liquid recycling, Stichopus japonicus water cooking liquid extract is applied in the preparation technology of Skin whitening care cosmetics simultaneously, the development in pluralism of Holothurian machining industry can be promoted.
Embodiment 2
A kind of Stichopus japonicus water cooking liquid extract Skin whitening care cosmetics, described skin care item are by Stichopus japonicus water cooking liquid extract, polyoxyethylene laurel ether, Cera Flava, siloxanes, Semen Maydis oil, vitamin E, methyl parahydroxybenzoate, glycerol, carbamide, aminoacid, lavandula angustifolia essence and deionized water are made, and the mass fraction of each component respectively Stichopus japonicus water cooking liquid extract 0.6 part needed for preparing these skin care item, polyoxyethylene laurel ether 3 parts, 10 parts of Cera Flava, siloxanes 5 parts, Semen Maydis oil 5 parts, vitamin E 0.1 part, glycerol 5 parts, methyl parahydroxybenzoate 0.1 part, 3 parts of carbamide, amino acid/11 part, lavandula angustifolia essence 0.08 part, deionized water is to 100 parts.
The preparation method of described Stichopus japonicus water cooking liquid reutilization technology and Stichopus japonicus water cooking liquid extract Skin whitening care cosmetics, comprises the following steps:
(1) described Stichopus japonicus water cooking liquid extract extracts separating step:
1. 100L Stichopus japonicus water cooking liquid adopts the centrifugal 30min remove impurity of disk centrifugal separator 10000r/min, then passes sequentially through microfiltration systems and ultrafiltration system, and wherein micro-filtration membrane material is ceramic membrane (Al2O3, 0.5 μm), ultrafiltration membrane material is ceramic membrane (Al2O3, 0.05 μm), the molecular weight cut coverage of ultrafilter membrane is 5000Da.Collect effluent.
2. will 1. in gained effluent with 5LNAK-9 macroporous adsorbent resin circulation absorption 4h (absorption 1h, in stop 0.5h).NAK-9 macroporous adsorbent resin after absorption is transferred in glass chromatography column, then washes with water to eluent colourless, and eluent discards.
3. with 3 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 15v%, eluent discards.
4. with 4 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 75v%, collect eluent, decompression and solvent recovery, lyophilizing, obtain Stichopus japonicus water cooking liquid extract.
5. adopting vanillin-ice acetic acid method to measure saponin mass content in Stichopus japonicus water cooking liquid extract is 45.02%, and sea cucumber polypeptide mass content is 48.58%, impurity 6.40%.
(2) according to formula precise Stichopus japonicus water cooking liquid extract 0.06g, polyoxyethylene laurel ether 0.3g, Cera Flava 1g, siloxanes 0.5g, Semen Maydis oil 0.5g, vitamin E 0.01g, methyl parahydroxybenzoate 0.01g, glycerol 0.5g, carbamide 0.3g, aminoacid 0.1g, lavandula angustifolia essence 0.008g, standby.
(3) polyoxyethylene laurel ether, Cera Flava, siloxanes, Semen Maydis oil, vitamin E, methyl parahydroxybenzoate are placed in beaker and are mixed and stirred for putting into, after uniformly, the ultrasonic Treatment 15min that frequency is 24kHz, as oil phase；Glycerol, carbamide, aminoacid and water are placed in beaker and are mixed and stirred for putting into, after uniformly, the ultrasonic Treatment 20min that frequency is 24kHz, as aqueous phase, and backward aqueous phase adds the Stichopus japonicus water cooking liquid extract of precise.
(4) by oil phase described in (3) with aqueous phase respectively at 95 DEG C of agitating heating 20min until observing that oil phase is all thoroughly mixed into transparence liquid with aqueous phase；Oil phase carries out churned mechanically while, aqueous phase is joined in oil phase；Reduce mixture temperature to 45 DEG C, and be added thereto to the methyl parahydroxybenzoate of a small amount of precise, continuously stirred make its uniform dissolution in the mixture；Continuing to reduce mixture temperature to 40 DEG C, drip the lavandula angustifolia essence of weighing wherein, continued mechanical stirring makes lavandula angustifolia essence uniform dissolution in the mixture；Finally reduce mixture temperature to room temperature, and to regulate its pH value with sodium hydroxide be 7.6, standby.
(5) being placed in hermetic container by mixture and carry out 100 DEG C of high temperature sterilizations, the time is 15min, namely prepares Stichopus japonicus water cooking liquid extract Skin whitening care cosmetics.
Above-mentioned skin care item are except having whitening effect, it is also possible to efficiently solve the gloomy skin staying nucleic acid relic to be formed because nucleic acid metabolism is smooth, make skin quality be improved significantly, and skin is not produced toxic and side effects.
The Stichopus japonicus water cooking liquid extract that the present invention relates to can also be applied to clean skin liquid, clean skin dew, cleansing cream, moisture retention liquid, moisturizing dew, moisturiser, skin lotion, skin cream, emollient cream, skin care liquid, cream for skin care, protective skin cream, cleansing milk, astringent, repair liquid, eye cream, in a series of Skin whitening care cosmetics formula of facial film.
The above; it is only the present invention preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; any those familiar with the art is in the technical scope of present disclosure; it is equal to replacement according to technical scheme and inventive concept thereof or is changed, all should be encompassed within protection scope of the present invention.
Claims (8)
1. the Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract, it is characterized in that, composition includes: in 100 parts of mass fractions, wherein, Stichopus japonicus water cooking liquid extract account for 0.1～1 part, natural oil lipid account for 5～20 parts, natural thickener account for 0～5 part, higher alcohol account for 0～5 part, emulsifying agent account for 1～25 part, preservative account for 0.1～0.5 part, essence account for 0.05～0.5 part, functional additive account for 0～5 part, water surplus；
Consisting of of described Stichopus japonicus water cooking liquid extract: selenka 30～90wt%, sea cucumber polypeptide 5～60wt%, impurity 5～10wt%；
Described natural oil lipid is Camellia oil, olive oil, Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, Semen Tritici aestivi germ oil, glycerol, cocoa butter, Oleum Cocois, Petiolus Trachycarpi oil, one or more mixing in Cera Flava, lanoline；
Described natural thickener is one or more mixing of natural plant starch, gelatin, guar gum, carrageenan, arabic gum, xanthan gum, alginate jelly；
Described higher alcohol is one or more mixing of lauryl alcohol, hexadecanol, octadecanol, hexadecanol, lanonol, cholesterol, plant sterol, octyldodecanol；
Described emulsifying agent is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene octadecanol, polyoxyethylene ether, one or more mixing of Vinlub 73；
Described preservative is one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate, benzyl alcohol, chloroxylenol, benzyl chlorophenol；
Described functional additive is one or more mixing of vitamin E, carbamide, aminoacid, hyaluronic acid, sheep placental extract；
Described essence is bouquet type composition or milk flavoured type essence.
2. according to claim 1 containing the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract, it is characterised in that the preparation method of described Stichopus japonicus water cooking liquid extract is:
After S1, the remove impurity by centrifugation of Stichopus japonicus water cooking liquid, pass sequentially through microfiltration systems and ultrafiltration system, collect effluent；
S2, with the NAK-9 absorption with macroporous adsorbent resin step S1 effluent obtained；Wash described adsorbent resin with water colourless to eluent, discard eluent；
S3, with 2～4 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 10～20v%, discard eluent；
S4, with 3～6 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 40～100v%, collect eluent；
S5, by step S4 collect eluent organic solvent is evaporated off after, in-70 DEG C～-40 DEG C lyophilization 24h～72h, prepare Stichopus japonicus water cooking liquid extract.
3. according to claim 1 containing the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract, it is characterised in that described natural oil lipid is one or more mixing in Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, glycerol, Petiolus Trachycarpi oil, Cera Flava；
Described natural thickener is one or more mixing in natural plant starch, gelatin, guar gum, xanthan gum；
Described higher alcohol is one or more mixing in hexadecanol, octadecanol；
Described emulsifying agent is stearic acid, sodium hydroxide, triethanolamine, siloxanes, one or more mixing of polyoxyethylene laurel ether；
Described preservative is one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate；
Described functional additive is one or more mixing in vitamin E, carbamide, aminoacid.
4. according to claim 1 containing the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract, it is characterised in that described bouquet type composition is rose essence, jasmin essence, lavandula angustifolia essence.
5. containing the preparation method of the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract described in claim 1, it is characterised in that concretely comprise the following steps:
S1, weigh Stichopus japonicus water cooking liquid extract, natural oil lipid, natural thickener, higher alcohol, emulsifying agent, preservative, essence, functional additive and water respectively according to formula, standby；
Described formula is: in 100 parts of mass fractions, wherein, Stichopus japonicus water cooking liquid extract account for 0.1～1 part, natural oil lipid account for 5～20 parts, natural thickener account for 0～5 part, higher alcohol account for 0～5 part, emulsifying agent account for 1～25 part, preservative account for 0.1～0.5 part, essence account for 0.05～0.5 part, functional additive account for 0～5 part, water surplus；
Consisting of of described Stichopus japonicus water cooking liquid extract: selenka 30～90wt%, sea cucumber polypeptide 5～60wt%, impurity 5～10wt%；
Described natural oil lipid is Camellia oil, olive oil, Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, Semen Tritici aestivi germ oil, glycerol, cocoa butter, Oleum Cocois, Petiolus Trachycarpi oil, one or more mixing in Cera Flava, lanoline；
Described natural thickener is one or more mixing of natural plant starch, gelatin, guar gum, carrageenan, arabic gum, xanthan gum, alginate jelly；
Described higher alcohol is one or more mixing of lauryl alcohol, hexadecanol, octadecanol, hexadecanol, lanonol, cholesterol, plant sterol, octyldodecanol；
Described emulsifying agent is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene octadecanol, polyoxyethylene ether, one or more mixing of Vinlub 73；
Described preservative is one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate, benzyl alcohol, chloroxylenol, benzyl chlorophenol；
Described functional additive is one or more mixing of vitamin E, carbamide, aminoacid, hyaluronic acid, sheep placental extract；
Described essence is bouquet type composition or milk flavoured type essence；
After S2, the natural oil lipid that step S1 is weighed, natural thickener, higher alcohol and emulsifying agent mix homogeneously, process 10～15min, 90～100 DEG C of agitating heating 20～30min with the frequency ultrasonic wave of 24kHz, obtain oil phase；After functional additive and water mix homogeneously, process 10～20min with the frequency ultrasonic wave of 24kHz, then add the step S1 Stichopus japonicus water cooking liquid extract weighed, 90～100 DEG C of agitating heating 20～30min, obtain aqueous phase；
S3, the aqueous phase obtained by step S2 join in described oil phase, stirring while adding；Reduce mixed solution temperature to 45～55 DEG C, add the step S1 preservative weighed, stir to uniform dissolution；Continue to reduce solution temperature to 30～40 DEG C, add the step S1 essence weighed, stir to uniform dissolution；Finally reduce solution temperature to room temperature, with sodium hydrate regulator solution pH value to 7.5～8.5, obtain mixed liquor；
S4, the mixed liquor 100 DEG C obtained by step S3 sterilization 10～15min, obtains the Skin whitening care cosmetics containing Stichopus japonicus water cooking liquid extract.
6. according to claim 5 containing the preparation method of the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract, it is characterised in that the preparation method of described Stichopus japonicus water cooking liquid extract is:
After S1, the remove impurity by centrifugation of Stichopus japonicus water cooking liquid, pass sequentially through microfiltration systems and ultrafiltration system, collect effluent；
S2, with the NAK-9 absorption with macroporous adsorbent resin step S1 effluent obtained；Wash described adsorbent resin with water colourless to eluent, discard eluent；
S3, with 2～4 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 10～20v%, discard eluent；
S4, with 3～6 times of column volumes of alcoholic solution eluting macroporous adsorbent resin of 40～100v%, collect eluent；
S5, by step S4 collect eluent organic solvent is evaporated off after, in-70 DEG C～-40 DEG C lyophilization 24h～72h, prepare Stichopus japonicus water cooking liquid extract.
7. according to claim 5 containing the preparation method of the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract, it is characterised in that described natural oil lipid is one or more mixing in Semen Maydis oil, Oleum Brassicae campestris, Oleum sesami, glycerol, Petiolus Trachycarpi oil, Cera Flava；
Described natural thickener is one or more mixing in natural plant starch, gelatin, guar gum, xanthan gum；
Described higher alcohol is one or more mixing in hexadecanol, octadecanol；
Described emulsifying agent is stearic acid, sodium hydroxide, triethanolamine, siloxanes, one or more mixing of polyoxyethylene laurel ether；
Described preservative is one or more mixing of methyl parahydroxybenzoate, ethylparaben, propyl p-hydroxybenzoate；
Described functional additive is one or more mixing in vitamin E, carbamide, aminoacid.
8. according to claim 5 containing the preparation method of the Skin whitening care cosmetics of Stichopus japonicus water cooking liquid extract, it is characterised in that described bouquet type composition is rose essence, jasmin essence, lavandula angustifolia essence.
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