CN110613650A

CN110613650A - Essence containing okra hydrolyzed glycoprotein SILKY-WSP and preparation method thereof

Info

Publication number: CN110613650A
Application number: CN201911023625.9A
Authority: CN
Inventors: 项光刚; 陈贤; 陶黎明; 周永传; 张阳; 安磊; 王跃强
Original assignee: Shanghai Jiayu Biological Technology Co Ltd
Current assignee: Shanghai Jiayu Biological Technology Co Ltd
Priority date: 2019-10-25
Filing date: 2019-10-25
Publication date: 2019-12-27

Abstract

The invention discloses essence containing okra hydrolyzed glycoprotein SILKY-WSP and a preparation method thereof. The essence consists of the following components in percentage by mass: 3.0 to 8.0 percent of glycerin, 0.1 to 0.3 percent of xanthan gum, 0.1 to 5.0 percent of trehalose, 0.2 to 2.0 percent of nicotinamide, 0.01 to 0.5 percent of okra extract, 0.2 to 0.5 percent of phenoxyethanol, 0.1 to 0.5 percent of octyl glycol and the balance of deionized water. The okra extract contained in the okra freeze-dried powder is a plant extract natural component, is safe, contains various similar structures, can play a role in multiple angles and multiple ways, and has good synergistic antioxidant and moisturizing effects.

Description

Essence containing okra hydrolyzed glycoprotein SILKY-WSP and preparation method thereof

Technical Field

The invention relates to an essence composition, belongs to the technical field of cosmetics, and particularly relates to an essence containing okra hydrolyzed glycoprotein SILKY-WSP and having antioxidant and moisturizing effects and a preparation method of the essence.

Background

Abelmoschus esculentus (Abelmmoschusus esculentus) is an annual herbaceous plant of Abelmmoschusus (Malvaceae) of Malvaceae, and is named as Abelmoschus esculentus, 'green ginseng', cavel, Bushencao, etc.

Okra (Abelmoschus Medicus L.) in Malvaceae is called vegetable king, has reputations of "green ginseng" and "plant Viagra" abroad, and has extremely high economic and medicinal values. About 15 species worldwide, distributed in africa, south europe, america and asia, with china mainly produced in south-east to south-west provinces, with 6 and 1 variety. The main varieties are Abelmoschus manihot (Linn.) Medicus, Abelmoschus manihot Abelmoschus moluschus Muliensis Feng, Abelmoschus manihot (Abelmoschus manihot) Abelmoschus esclus (Linn.) Moench, Abelmoschus manihot Merr, Abelmoschus manihot (Abelmoschus manihot) Merr, Abelmoschus manihot Wall, Hibiseu manihot L.

The okra plant has effects of clearing heat, promoting diuresis, removing toxic substance, removing blood stasis, promoting blood circulation, moistening dryness, dredging channels, promoting granulation, regulating stomach function, and resolving food stagnation, and can be used for treating carbuncle, cough due to lung heat, lithangiuria, furuncle, parotitis, icterohepatitis, fracture, burn, scald, and galactostasis. Among them, most plants are used as top-quality vegetables, 11 kinds of national herbs are used, and at least 7 parts including Bencao gang mu are described as the national herbs. Okra is rich in nutrients such as vitamins (A, B1, B2, C and E), minerals, flavonoids, fatty acids, polysaccharides, crude proteins and amino acids, and other compounds. Moreover, these chemicals have been considered to have a wide variety of biological functions including anti-aging, anti-oxidation, anti-inflammatory, anti-fatigue, anti-mutagenic, anti-tumor proliferation and immune enhancement for human health, with tremendous potential for development.

At present, the skilled person has obtained okra extract by subjecting okra to an extraction process, and has applied it to cosmetics. The traditional extraction method is a steam distillation method, which has the defects of simple equipment, low cost and simple and convenient operation, but has the defects of large damage to active ingredients and high impurity content.

Therefore, how to fully utilize the functional components contained in the okra and apply the effective components to various skin care products is a technical problem which needs to be solved urgently by the technical personnel in the field.

Disclosure of Invention

The invention aims to provide essence containing okra hydrolyzed glycoprotein SILKY-WSP, which fully utilizes the obvious antioxidant effect of an okra extract on skin, has good moisturizing, lubricating and soothing effects, has high product safety and is suitable for various crowds.

The invention also aims to provide a preparation method of the essence containing okra hydrolyzed glycoprotein SILKY-WSP, so that the active ingredients (okra hydrolyzed glycoprotein) in the product are retained to the maximum extent.

The purpose of the invention and the technical problem to be solved are realized by adopting the following technical scheme. The essence containing okra hydrolyzed glycoprotein SILKY-WSP provided by the invention comprises the following components in percentage by mass:

3.0 to 8.0 percent of glycerin, 0.1 to 0.3 percent of xanthan gum, 0.1 to 5.0 percent of trehalose, 0.2 to 2.0 percent of nicotinamide, 0.01 to 0.5 percent of okra extract, 0.2 to 0.5 percent of phenoxyethanol, 0.1 to 0.5 percent of octyl glycol and the balance of deionized water.

Further, the essence containing okra hydrolyzed glycoprotein SILKY-WSP comprises the following components in percentage by mass:

3.0 to 5.0 percent of glycerin; 0.1 to 0.2 percent of xanthan gum; trehalose 0.3% -5.0%; 0.5 to 1.5 percent of nicotinamide; 0.05 to 0.15 percent of okra extract; 0.2 to 0.3 percent of phenoxyethanol; 0.45 to 0.5 percent of octyl glycol; the balance being deionized water.

5.0% of glycerin; 0.1 percent of xanthan gum; 0.5 percent of trehalose; 0.5 percent of nicotinamide; okra extract 0.15%; 0.2 percent of phenoxyethanol; 0.5 percent of octyl glycol; the balance being deionized water.

Further, the preparation method of the okra extract comprises the following steps:

1) after cleaning fresh okra, pouring out the okra slightly, cleaning with purified water every time, and stirring slightly in the cleaning process every time; then cutting with scissors;

2) conveying okra raw material fragments into a supercritical extraction kettle, and introducing carbon dioxide fluid into the supercritical extraction kettle for extraction; taking out the extraction mixture from the outlet of the supercritical extraction kettle, and inputting the mixture into a supercritical separation kettle for separation;

3) diluting the separated product with water, directly heating and distilling with fire, and condensing vapor to obtain okra extract;

4) filtering the above Abelmoschus esculentus extract with filter membrane with pore diameter less than 0.6 μ M; concentrating the supernatant until the protein concentration in the concentrated solution is 80 +/-0.5 mu g/ml; filtering the concentrated supernatant with a filter membrane with pore diameter less than 0.3 μ M;

5) freeze-drying the okra extract filtered by the filter membrane to obtain the okra freeze-dried powder.

Further, in step 1), the fresh okra is selected from high-quality fresh okra and okra is finishedCleaning the whole fresh okra without impurities by using purified water, wherein the purified water is used for cleaning for three times by using 5 times of purified water each time; the size of each piece of the cut pieces is less than or equal to 5mm²。

Further, in the step 2), the raw material fragments are conveyed to a supercritical extraction kettle, carbon dioxide fluid is input into the supercritical extraction kettle, the flow rate of the carbon dioxide is 10-20 kg/h, the extraction pressure is adjusted to be 25-30 MPa, the extraction temperature is 32 ℃, and extraction is carried out for 5 hours.

Further, in the step 2), in the separation process, the pressure is adjusted to be 3-6 MPa, the temperature is adjusted to be 35-36 ℃, and the separation is carried out for 2 hours.

Further, in the step 3), the separated product is mixed according to a feed-liquid ratio of 1: and adding water for dilution at 12 g/L.

Further, in the step 5), during the freeze-drying treatment, the temperature is-30 ℃ to-50 ℃, the vacuum degree is 50-200Pa, and the freeze-drying time is 15-20 hours.

Further, the preparation method of the essence containing okra hydrolyzed glycoprotein SILKY-WSP comprises the following steps:

1) wetting xanthan gum with glycerol, adding into a water phase pot, stirring, sequentially adding trehalose, heating to 82 deg.C, and keeping the temperature for 12 min until completely dissolving;

2) keeping the stirring speed of an emulsifying pot at 350 r/min, sucking the components obtained in the step 1) in vacuum, homogenizing at low speed for 3 min, and cooling; cooling to 55 deg.C, adding nicotinamide into the emulsifying pot, stirring, and mixing; then adding the okra extract into an emulsifying pot, starting stirring, and uniformly stirring;

3) stirring and cooling to below 42 ℃, sequentially adding phenoxyethanol and caprylyl glycol, and stirring for 15 minutes until uniform; cooling to 37 deg.C, filtering and discharging; and (5) inspecting, discharging and filling.

By means of the technical scheme, the invention has the following advantages and beneficial effects:

1) the okra flower extract has strong effects of resisting oxidation and removing hydroxyl free radicals, and is a promising health-care plant with antioxidant activity and anti-aging to be developed. Research shows that proper intake of okra fruit powder can obviously improve the oxidation resistance of D-galactose-induced aging model mice and delay aging.

2) The okra hydrolyzed glycoprotein SILKY-WSP-containing water-soluble protein preparation disclosed by the invention belongs to natural ingredients extracted from plants, is safe, contains various similar structures, can play a role in multiple angles and multiple ways, and has good synergistic antioxidant and moisturizing effects.

3) Experiments show that the essence product containing okra hydrolyzed glycoprotein SILKY-WSP shows excellent skin feel, is colorless and tasteless, can be prepared into a skin care product without artificially synthesized essence, and has higher safety; tests prove that the product has obvious antioxidant and high-efficiency moisturizing beauty effects, and the essence product rich in the okra extract has good market application prospect.

Drawings

Fig. 1 is a graph showing the change of stratum corneum of skin after the essence product of example 4 of the present invention is applied.

FIG. 2a is a diagram showing the lack of water in the stratum corneum layer of the skin before the essence product of example 5 of the present invention is applied;

FIG. 2b is a graph showing the hydration of the stratum corneum of the skin 1 hour after the essence product of example 5 of the present invention is applied;

fig. 3 is a graph showing the change of moisture content of stratum corneum after applying the essence products of examples 4 to 6 of the present invention and the comparative example for different periods of time.

Fig. 4a-4b are images showing the appearance of facial skin of two different volunteers after applying the essence product of example 5 of the present invention.

Detailed Description

The present invention will be described in more detail below with reference to specific preferred embodiments and drawings, but the present invention is not limited to the following embodiments.

The okra extract (containing hydrolyzed glycoprotein SILKY-WSP) adopts a supercritical extraction method and a freeze-drying technology, the whole process is aseptic operation, an organic solvent is not used, and no chemical substances and germs are left in the freeze-dried powder. The method can rapidly extract large amount of high purity extract without destroying natural active ingredients, and has high yield and less impurities. The plant extracts natural ingredients, is safe, contains various similar structures, can play the role of the plant in multiple angles and multiple ways, and has good synergistic skin care effect.

In the following examples of the present invention, the raw material of the okra extract is selected from okra; the dosage form of the okra extract adopts okra freeze-dried powder.

Example 1 (preparation of okra extract)

A preparation method of an okra extract comprises the following steps:

1) selecting high-quality fresh, complete and impurity-free fresh okra 500g, cleaning with purified water, pouring out okra gently, cleaning with 5 times of purified water each time for three times, stirring gently in each cleaning process, and cutting with scissors until the size of each piece is less than or equal to 5mm²；

2) Conveying the raw material fragments into a supercritical extraction kettle, inputting carbon dioxide fluid into the supercritical extraction kettle, adjusting the flow rate of the carbon dioxide to be 10kg/h, adjusting the extraction pressure to be 25MPa, and extracting at the extraction temperature of 30 ℃ for 5 hours;

3) taking out the extraction mixture from the outlet of the supercritical extraction kettle, inputting the mixture into a supercritical separation kettle, adjusting the separation pressure to be 3MPa and the temperature to be 35-36 ℃, and separating for 2 hours;

4) and (3) mixing the separated products according to a material-liquid ratio of 1: adding 12g/L of the okra extract into water, heating and distilling for 2 hours by direct fire, and condensing and recycling steam to obtain the okra extract;

5) filtering the okra extract with a filter membrane with the pore diameter of less than 0.6 mu M; concentrating the supernatant until the protein concentration in the concentrated solution is 79.5 mu g/ml; filtering the concentrated supernatant with a filter membrane with pore diameter less than 0.3 μ M;

6) during the freeze-drying treatment, the freeze-drying temperature is-30 ℃, the vacuum degree is 50Pa, and the freeze-drying time is 15 hours, so that the okra freeze-dried powder is obtained.

Example 2 (preparation of okra extract)

2) Conveying the raw material fragments into a supercritical extraction kettle, inputting carbon dioxide fluid into the supercritical extraction kettle, adjusting the flow rate of the carbon dioxide to be 20kg/h, adjusting the extraction pressure to be 30MPa, and extracting at the extraction temperature of 32 ℃ for 5 hours;

3) taking out the extraction mixture from the outlet of the supercritical extraction kettle, inputting the mixture into a supercritical separation kettle, adjusting the separation pressure to 6MPa and the temperature to 36 ℃, and separating for 2 hours;

5) filtering the okra extract with a filter membrane with the pore diameter of less than 0.6 mu M; concentrating the supernatant until the protein concentration in the concentrated solution is 80.5 mug/ml; filtering the concentrated supernatant with a filter membrane with pore diameter less than 0.3 μ M;

6) during the freeze-drying treatment, the freeze-drying temperature is-50 ℃, the vacuum degree is 200Pa, and the freeze-drying time is 20 hours, so that the okra freeze-dried powder is obtained.

Example 3 (preparation of okra extract)

2) Conveying the raw material fragments into a supercritical extraction kettle, inputting carbon dioxide fluid into the supercritical extraction kettle, adjusting the flow rate of the carbon dioxide to be 15kg/h, adjusting the extraction pressure to be 28MPa, and extracting at the extraction temperature of 32 ℃ for 5 hours;

3) taking out the extraction mixture from the outlet of the supercritical extraction kettle, inputting the mixture into a supercritical separation kettle, adjusting the separation pressure to 4.5MPa and the temperature to 35 ℃, and separating for 2 hours;

5) filtering the okra extract with a filter membrane with the pore diameter of less than 0.6 mu M; concentrating the supernatant until the protein concentration in the concentrated solution is 80 mug/ml; filtering the concentrated supernatant with a filter membrane with pore diameter less than 0.3 μ M;

6) during the freeze-drying treatment, the freeze-drying temperature is-40 ℃, the vacuum degree is 100Pa, and the freeze-drying time is 18 hours, so that the okra freeze-dried powder is obtained.

The okra hydrolyzed extract products of examples 1 to 3 of the present invention (name: SILKY-WSP okra hydrolyzed glycoprotein, product No. JY2002-2004) were tested, and the results are shown in Table 1 below:

TABLE 1 SILKY-WSP okra hydrolysis glycoprotein index results

Analysis item	Test standard	Test results
			Shape (conventional parameters)	White powder/white-like powder	Qualified
Smell (conventional parameters)	Characteristic odor	Qualified
			Physical and chemical properties: pH (0.2% aqueous solution, 25 ℃ C.)	5.0-7.0	6.4
Physical and chemical properties: soluble (25 ℃ C., ≧ 1g/100ml water)	≥99％	99.2％
			Weight loss on dry weight (physical and chemical properties)	≤5.0％	3.9％
Relative average molecular weight (physical and chemical properties)	≥0.9×10⁶	0.95×10⁶
			Total number of bacteria (microbiological indicator)	<100cfu/g	Qualified
Mold and yeast (microbiological indicator)	<10cfu/g	Qualified
			Escherichia coli (microbiological indicator)	Cannot be detected	Qualified
Pseudomonas aeruginosa (microbiological indicator)	Cannot be detected	Qualified
			Staphylococcus aureus (microbiological indicator)	Cannot be detected	Qualified
Lead (heavy metal)	<10ppm	Qualified
			Arsenic (heavy metal)	<2ppm	Qualified
Mercury (heavy metal)	<10ppm	Qualified
			Cadmium (heavy metal)	<5ppm	Qualified

From the above conclusions in table 1 it can be seen that: the products of examples 1-3 of the present invention were all qualified products after inspection.

INCI name: an extract of the fruit of Coffea yellow Quinus (Abelmoschus esculentus).

The pH range is as follows: 5.0-7.0; recommended dosage is as follows: 0.05 to 0.3 percent. The application range is as follows: water, facial mask, essence, lotion, cream, body lotion, shampoo, face cleanser, toothpaste, etc.

Temperature stability: steaming at 120 deg.C for 40min under high temperature and high pressure to reduce viscosity and maintain pH and light transmittance; heating at 80 deg.C for 30min, and keeping viscosity, pH and light transmittance unchanged.

pH stability: the viscosity of the SILKY-WSP aqueous solution is kept unchanged under the condition of pH value of 5.5-9.5.

Strong light stability: 4500LX, 40 ℃, 10 days, the product water solution has good light stability.

Long-term stability: the solid powder is stored for 24 months at normal temperature.

The okra extract product of the invention has the following characteristics:

1) the invention adopts advanced supercritical extraction technology and freeze-drying technology, simplifies the production process, improves the working efficiency, reserves more active ingredients of the product, has less impurities and has far better product efficacy than the traditional steam distillation method.

2) The okra extract has a strong scavenging effect on superoxide anion free radicals, hydroxyl free radicals, dibenzo-bitter acyl free radicals and the like, and has an obvious dose-effect relationship. Besides the obvious antioxidant effect, the product also has the good effects of moisturizing, lubricating and relieving, has high safety and is suitable for various crowds.

3) The product has wide source and low cost, and the production capacity is not limited by the production region and season of the raw materials, and can be stably produced for a long time.

The okra extract prepared by the supercritical extraction technology and the freeze-drying technology has higher activity, has obvious effects of resisting oxidation and removing free radicals, has very high-efficiency moisturizing and lubricating effects, can be applied to various functional cosmetics, and has excellent effects of resisting oxidation, moisturizing, improving skin feel and the like.

The okra hydrolyzed glycoprotein disclosed by the invention has the effects of resisting oxidation, moisturizing, nourishing skin and mucosa, enhancing the smooth feeling of a product, providing good skin feeling, and having the functions of relieving, thickening and stabilizing.

Examples 4 to 6 (essence containing okra extract)

The okra extracts (okra hydrolyzed glycoprotein) prepared in the above examples 1 to 3 were added to the examples 4 to 6, respectively, to prepare essence composition skin care products. The raw material composition formula is shown in the following table 2.

The following percentages are all weight percentages.

Table 2 formula compositions (% by weight) of essences of inventive examples 4 to 6 and comparative examples 1 to 3

The preparation method of the essence composition described in table 2 comprises the following process steps:

1) wetting xanthan gum in the component A with glycerol, adding into a water phase pot, stirring, sequentially adding other rest components A, heating to 82 deg.C, and keeping the temperature for 12 min until completely dissolving;

2) keeping the stirring speed of the emulsifying pot at 350 r/min, sucking the component A in vacuum, homogenizing at low speed for 3 min, and cooling;

3) cooling to 55 deg.C, adding the raw materials of component B into emulsifying pot, stirring, and mixing;

4) adding the component C into an emulsifying pot, starting stirring, and uniformly stirring;

5) stirring and cooling to below 42 ℃, adding the component D, stirring for 15 minutes until the mixture is uniform, and filtering and discharging when the temperature is reduced to 37 ℃; and (5) inspecting, discharging and filling.

The following are related contents of the effect application test examples, respectively.

Examples of Effect test

1. Moisture retention study case one

The test method comprises the following steps: the essence product of the embodiment 4 of the invention is smeared on the cheek skin under the eyes;

the number of people: 8 persons;

testing parts: the lower cheek of the eye;

and (3) testing environment: humidity is 40%, and temperature is 20 ℃;

testing an instrument: courage + Khazaka Cologne Germany, Multi skin test centre MC 750;

the results of fig. 1 show: in the variation range of 0-4h, the essence product containing silk-WSP okra hydrolyzed glycoprotein can improve the moisture content of the skin, and the moisturizing effect is stable; and reached the highest peak at 0.5 hours. The blank water control example has a significantly poorer moisturizing effect than the essence product of the invention.

2. Moisture retention study case two

The test method comprises the following steps: the essence product of the embodiment 5 of the invention is smeared on the cheek skin under the eyes;

the tester: 26 years old and female; the skin is in a moisture-keeping state before and after use;

testing parts: the lower cheek of the eye;

and (3) testing environment: humidity is 40%, and temperature is 20 ℃;

the results in fig. 2a show that the stratum corneum is water-deficient before application of the serum product of example 5 of the present invention; the water content in the T and U parts is 'very insufficient'.

The results in fig. 2b show that the moisture content of the stratum corneum increased 1 hour after application of the serum product of example 5 of the present invention; the water content of the T part and the U part is proper; the essence containing silk-WSP okra hydrolyzed glycoprotein can improve the moisture content of the skin and has stable moisturizing effect.

3. Moisture retention study case three

The test method comprises the following steps: 70ul of the sample (serum products according to examples 4-6 of the present invention) was applied to the medial aspect of the wrists. The measured value of the electric conductivity before application was set to 1, and the change in the value was observed before application and after application for 5, 15, 30, and 60 minutes. Sequentially evaluating the moisturizing facial skin of the samples;

the tester: 30 persons, 10 persons in male and 20 persons in female, wherein the age is 21-49 years, and the volunteer selection criteria of the subject are met; the skin is in a moisture-keeping state before and after use;

and (3) testing results: the change in the value of the conductivity was observed, and the result is shown in FIG. 3.

The conclusion of fig. 3 illustrates: examples 4-6 showed significantly higher moisture retention after application than control 1, indicating that the essence product containing silk-WSP okra hydrolyzed glycoprotein can increase the moisture content of the skin and has stable moisture retention effect.

4. Evaluation of antioxidant (free radical) Effect

Test samples: examples 4-6, comparative examples 1-3;

the test purpose is as follows: the efficacy of the skin care product in terms of antioxidant activity was evaluated by continued use of the skin care product under normal conditions by adult female volunteers.

Age of volunteer: women between 25-44 years old;

the number of volunteers: 180 persons;

facial skin of volunteers: dark and dull facial complexion;

the product use frequency is as follows: the face is cleaned and then evenly smeared, 2 times a day, once in the morning and evening, four weeks after the product is used and eight weeks after the product is used.

As shown in fig. 4a and 4b, the facial skins of two different volunteers are presented with graphs respectively, and the effects of the essence product containing silk-WSP okra hydrolyzed glycoprotein on improving skin brightness and firming skin are demonstrated after the product is used for four weeks and after the product is used for eight weeks.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. An essence containing okra hydrolyzed glycoprotein SILKY-WSP is characterized in that: the essence consists of the following components in percentage by mass:

3.0 to 8.0 percent of glycerin;

0.1 to 0.3 percent of xanthan gum;

trehalose 0.1% -5.0%;

0.2 to 2.0 percent of nicotinamide;

0.01 to 0.5 percent of okra extract;

0.2 to 0.5 percent of phenoxyethanol;

0.1 to 0.5 percent of octyl glycol;

the balance being deionized water.

2. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 1, wherein: the essence consists of the following components in percentage by mass:

3.0 to 5.0 percent of glycerin;

0.1 to 0.2 percent of xanthan gum;

trehalose 0.3% -5.0%;

0.5 to 1.5 percent of nicotinamide;

0.05 to 0.15 percent of okra extract;

0.2 to 0.3 percent of phenoxyethanol;

0.45 to 0.5 percent of octyl glycol;

the balance being deionized water.

3. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 1, wherein: the essence consists of the following components in percentage by mass:

5.0% of glycerin;

0.1 percent of xanthan gum;

0.5 percent of trehalose;

0.5 percent of nicotinamide;

okra extract 0.15%;

0.2 percent of phenoxyethanol;

0.5 percent of octyl glycol;

the balance being deionized water.

4. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to any one of claims 1 to 3, wherein: the preparation method of the okra extract comprises the following steps:

1) after cleaning fresh okra, pouring out the okra slightly, cleaning with purified water each time, stirring slightly in the cleaning process each time, and then shearing with scissors;

5. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 4, wherein: in the step 1), selecting high-quality fresh, complete and impurity-free fresh okra, and cleaning with purified water, wherein the purified water is used for cleaning three times by 5 times each time; the size of each piece of the cut pieces is less than or equal to 5mm²。

6. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 4, wherein: in the step 2), the raw material fragments are conveyed into a supercritical extraction kettle, carbon dioxide fluid is input into the supercritical extraction kettle, the flow rate of the carbon dioxide is 10-20 kg/h, the extraction pressure is adjusted to be 25-30 MPa, the extraction temperature is 32 ℃, and extraction is carried out for 5 hours.

7. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 4, wherein: in the step 2), in the separation process, the pressure is adjusted to be 3-6 MPa, the temperature is adjusted to be 35-36 ℃, and the separation is carried out for 2 hours.

8. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 4, wherein: in the step 3), the separation product is mixed according to a feed-liquid ratio of 1: adding water for dilution at 12 g/L; the time for the direct fire distillation was 2 hours.

9. The essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claim 4, wherein: in the step 5), during freeze-drying treatment, the temperature is-30 ℃ to-50 ℃, the vacuum degree is 50-200Pa, and the freeze-drying time is 15-20 hours.

10. The method for preparing essence containing okra hydrolyzed glycoprotein SILKY-WSP according to claims 4 to 9, comprising the steps of: