TW200930411A - Manufacturing method and application of extract of Abelmoschus esculentus having humidity preservation function - Google Patents
Manufacturing method and application of extract of Abelmoschus esculentus having humidity preservation function Download PDFInfo
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200930411 九、發明說明： 【發明所屬之技術領域】 本發明係關於一種萃取物，尤其係指一種萃取自黃秋 蔡之萃取物，該萃取物具有多醣體及蛋白質，具有保濕功 能，且為植物性，對於人體皮膚具低敏感性、安全性，安 定性與保濕有效性。 【先前技術】 黃秋葵屬錦葵科（Λ/fa/vaceae)秋葵屬，一年生草本植 物，學名為 4/)e/m〇sc/7t；s escw/enius (L.) Moenc/?.，又名 為蘋田、食用秋葵或倭克拉，原產熱帶非洲。黃秋葵主要 可:用品種約# 7種，觀賞品種全株呈褐紅色。產地分佈 在卷化、雲林、嘉義等縣境内。每年4〜9月是盛產期，果 實以供應餐廳為主，一般市場並不普遍。 ❹ 黃秋葵的果實為長條形如羊角，莢内有許多小黑豆，故 羊角菜或羊角豆，為歐美人士喜愛的果菜之一。黃秋 /葵的嫩果含有水溶性纖維及黏蛋白，纟中黏蛋自為具有高 刀子量的醣蛋白’其為一種呈現黏滑的物質，具整腸健胃 功效，根據農委會農業統計之產銷資料，台灣近五年拍賣 市場黃秋葵交易量統計， T匕由1997年297公噸增加至2000 Α噸’可知目前台灣黃秋葵的生產與消費人口正逐 漸增加中。 在1954年，_丨斷_㈤加等人將抽取自黃秋 5 200930411 • 蔡中的膽蛋白質黏蛋白之黏質成份酸水解得到多醣體，該 多醣體組成為半乳糖、鼠李糖和半乳糖搭酸，並且從黏質 成份得到1,4鍵結的半乳二糖（galactobiose)和2-0-(D-半 礼糖酸·酸）-L-鼠李糖（2-0- ( D-galactopyranosyiuronic acid) -L-rhamnose)。（Whistler，R.L· And Conrad， H.E.,1954, 2-0- ( D-GalactoPyranosyluronic Acid) -L- rhamnose from Okra Mucilage.C/jem.Soc 76:3544-❹ 3546.) 而在1956年’ Amin等人提出黃秋葵萃取物組成含量 為半乳糖含量80%、鼠李糖10%、阿拉伯糖3%、半乳糖 醛酸6% ;其主要成份是1，4鍵結的半乳糖和1,2鍵結的 鼠李糖殘基。（Amin,E.S·, 1956，The Mucilages of Hibiscus esculentus ( Okra or Bamia fellahi ) and Corchorus olitorius olitorius ( Mulukhia) J. Chem. Soc. 78:828-832.) 在1977年’ Woolfe等人提出黃秋葵抽取黏質成份， Θ 係由酸的多聽體和許.多蛋白質、礦物質所構成，純化過程 中這些物質也不會有明顯減少。水解後利用膠體層析法分 析可得半乳糖醛酸、半乳糖、鼠李糖和葡萄糖等成份（含 量比例為1·3 : 1.0 : 0·1 : 0·1 );且其研究進一步確認該 黏質成份為中性。（Woolfe,M.L.，Chaplin，M.F.and200930411 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD The present invention relates to an extract, in particular to an extract extracted from Huangqiu Cai, which has a polysaccharide and a protein, has a moisturizing function, and is a plant. Sexuality, low sensitivity, safety, stability and moisturizing effectiveness for human skin. [Prior Art] Okra is a Malvaceae (Λ/fa/vaceae) Okra, an annual herb, with the scientific name 4/)e/m〇sc/7t; s escw/enius (L.) Moenc/?. Also known as Ping Tian, edible okra or 倭 ,, native to tropical Africa. The main okra can be: use about 7 species, the ornamental variety is brownish red. The distribution of production areas is in the counties of Fuhua, Yunlin and Chiayi. Every year from April to September is a prolific period. It is mainly based on the supply of restaurants. The general market is not universal. ❹ The fruit of the okra is long in shape like a shofar. There are many small black beans in the pod. Therefore, croissants or croissants are one of the favorite fruits and vegetables in Europe and America. Huangqiu/Kwai's tender fruit contains water-soluble fiber and mucin, and the sticky egg in the sputum is a glycoprotein with a high knife amount. It is a kind of sticky and slippery substance with a stomach function. According to the Agriculture Committee of Agriculture Statistics on production and sales, Taiwan's auction market statistics of Okra in the past five years, T匕 increased from 297 metric tons in 1997 to 2000 tons. It is known that the production and consumption of Taiwan's okra is gradually increasing. In 1954, _ 丨 _ _ (five) plus et al. will be extracted from Huang Qiu 5 200930411 • Cai Zhong's viscous component of the biliary protein mucin is acid hydrolyzed to obtain a polysaccharide body composed of galactose, rhamnose and half Lactose is acidified, and 1,4-bonded galactobiose and 2-0-(D-semi-glycolic acid)-L-rhamnose (2-0- D-galactopyranosyiuronic acid) -L-rhamnose). (Whistler, RL· And Conrad, HE, 1954, 2-0- (D-GalactoPyranosyluronic Acid) -L- rhamnose from Okra Mucilage.C/jem.Soc 76:3544-❹ 3546.) And in 1956 'Amin et al. It is suggested that the content of the extract of okra is 80% galactose content, 10% rhamnose, 3% arabinose and 6% galacturonic acid. The main components are 1,4-bonded galactose and 1,2 bond. Rhamnose residue. (Amin, ES·, 1956, The Mucilages of Hibiscus esculentus ( Okra or Bamia fellahi ) and Corchorus olitorius olitorius ( Mulukhia ) J. Chem. Soc. 78: 828-832.) In 1977 ' Woolfe et al. proposed the okra extract The substance, Θ is composed of acid multi-audio and Xu. Multi-protein, minerals, and these substances are not significantly reduced during the purification process. After hydrolysis, the components such as galacturonic acid, galactose, rhamnose and glucose can be obtained by colloidal chromatography (content ratio is 1:3: 1.0: 0·1: 0·1); The viscosity component is neutral. (Woolfe, M.L., Chaplin, M.F.and
Otchere,G.，1977，Studies on the mucilages extracted form okra fruit ( Hibiscus esculentus L.) and baobab (Adansonia L. ) .J.Sci.Food Agric. 28:519-529.) 在1 987年，Bhat和Tharanathan對黃秋葵之黏質成 6 200930411 • 份提出較複雜之組成’除了半乳糖、鼠李糖、半乳糖醛酸 之組成外，再將糖類細分為葡萄糖、甘露糖、阿拉伯糖和 木糖》(Bhat,U.R. andTharanathan，R.N.，1987,Fi/nci/ona/ Properties of Okra ( Hibiscus esculentus ) mucilage.Starch 39:165-167.) 目前黃秋葵多為食用，關於黃秋葵中所含的黏質成分 的分析已揭露於前述相關前案，然而該黏質成分之應用， ❹ 目前多為直接食用黃秋葵’尚無任何研究顯示將黃秋葵中 的黏質成分應用於化妝或保養用品的成分。 而目前一般市面上標榜具有保濕效果的化妝品多以透 明質酸作為其保濕成分’透明質酸又稱玻尿酸或糖醛酸 (hyaluronic acid，簡稱HA)，係由葡萄糖醛酸-n乙醯 氨基葡萄糖為雙糖單位組成的直鏈高分子多醣。平均分子 量介於10萬到1 〇〇〇萬道爾頓（Da|t〇n)之間，對於皮膚 、’且織和結構上的整體保養’和細胞間的輸送過程都扮演很 ❹ 重要的功能；其尚能應用於化妝品中或應用在皮膚保濕及 傷口 的癒合（Balazs，E_ A_ and Band，P.，1984, /7ya/ur〇n/c acid ../is siruciure use·，Toil, 99(6)H65 -72). 目前商品型態通常是鈉鹽型式，所以稱之為透明質酸鈉。 透明質酸廣泛存在動物組織細胞間質和眼玻璃體中，生理 功能是保水和潤滑，是一種大分子粘多糖廣泛存在於生物 體的結締組織中。在不同組織中透明質酸的生理作用有所 不同’例如：在皮膚中主要作用為保濕功能，在關節裏主 要作為潤滑作用，在血管壁中主要是調節通透性。另外透 7 200930411 •曰月質酸亦為聚負離子電解質，其分子上所帶的大量負电荷 可調節周圍正負離子的濃度，抑制多種酶的活性。 早期研究認為透明質酸僅是一種細胞間隙填充物，所 以沒有引起人們太多重視’近幾年隨著對透明質酸的結 構、性能、生物功能性的深入認識，透明質酸已成為細胞 生物、病理、免疫學等領域的研究焦點。因具有良好黏彈 性、可塑性、渗透性和生物相容性，所以能夠廣泛應用於 ❹臨床醫學和化妝品的生產。近年來透明質酸市場需求與日 俱增’表現出良好的市場潛力和廣闊的應用前景。其應用 範輕據透明質酸之分子量來作劃分：小分子量的透明質 馱應用在化妝品上；大分子量的透明質酸應用在生醫材料 上。 由於歐美及日本近年來皆有狂牛症的案例產生，又基 於宗教不殺生的觀念及可能有病菌殘留的疑慮，取自動物 而應用於化妝品之保濕產物，如骨膠原、膠原蛋白、胎盤 © $物質已漸漸被歐美國家捨棄，改以從植物中能取得的物 質代替，大體而言，多醣體成份有逐漸取代原有動物來源 產物，成為保濕原料來源主流。 多畴體在肌膚中會隨著年齡增長而逐漸減少，因此在 保養品中添加適量多醣體原料可以適當增加肌膚保濕功 月匕，而台灣及東南亞地區屬較潮濕及悶熱地方，所以化妆 。〇業者都偏好清灸、吸濕性之保濕成份，因此所選定植物 αο種來源及其萃取成份需能夠符合清爽、保濕、吸濕性之 條件’除次之外’更須具備有生產快速，原料取得容易、 200930411 - 便且等條件，故如何開發屮 ^ 1發出具有上述條件，並使用植物為 ''之原料取代由動物為來源取得之保濕原料，以提高天 ::植物在化妝品產業的附加價值，還有加工處理技術與製 備可行性研究，實具研究的必要。 【發明内容】 ―本發明的主要目的為提供一種具有保濕功能之黃秋蔡 〇 卒取物，該萃取物呈現一種黏滑物質的形式，本發明為植 物性，含有多醣體及蛋白質，而具有保濕的效果，故可取 代般動物性之保濕原料而成為一般化妝或保養品的添加 物’本發明經過測試後證實對人體皮膚不會產生過敏反 應’具有良好的安全性與保濕有效性。 + ★為達上述目的，本發明提供一種具有保濕功能之黃秋 葵萃取物的萃取方法，其係由下列步驟製備而成： 取得黄秋葵以及萃取溶液； & 將黃秋葵和萃取溶劑以i : i〜丄：i 〇的比例混合 以1 — 1 0 0 °c，進行萃取反應；以及 收集初萃物溶液，並將其過濾以獲得具有保濕功能之 黃秋葵萃取物。 較佳的’該黃秋葵萃取物具有多酷體。 較佳的，該黃秋葵萃取物具有蛋白質。 較佳的’該取得黃秋葵尚包含將其清洗、去皮及輾碎。 較佳的，該萃取溶劑為極性溶液。 較佳的’該萃取溶劑為純水、乙醇或1，3 丁二醇。 9 200930411 較佳的’該萃取溶劑為 較佳的，該卒取溶劑為 較佳的，該萃取溶劑為 0%體積百分比之1,3 丁_ 醇。 1 0 0 %體積百分比之純水。 1 0 0%體積百分比之乙醇。 6 0 %體積百分比之純水、2 醇以及2 0%體積百分比之6 較佳的，該萃取溶劑為8 0 %體積百分比之純水、2 0%體積百分比之1，3 丁二醇。 較佳的，該萃取溶劑為8 0 %體積百分比之純水以及 2 0%體積百分比之乙醇β 較佳的，以8 〇。(：配置該萃取溶劑。 較佳的，以1 〇 0 °C配置該萃取溶液。 較佳的’該黃秋葵和萃取溶劑混合比例分別為1 : 1 0 ° 較佳的’該黃秋葵和萃取溶劑混合比例分別為1 : 1 0。 〇 較佳的，該黃秋葵和萃取溶劑混合後係進行迴徒浪縮 萃取。 較佳的，該黃秋葵和萃取溶劑混合後係以減壓迴旋濃 縮機：EYELA，SB-1 000進行迴旋濃縮萃取。 較佳的，進一步使用一有效載體。 較佳的，該載體為微脂體。 本發明尚關於一種使用上述萃取方法萃取出之@秋葵 萃取物的保濕原料。 較佳的，該保濕原料進一步包含一界面活性刻 10 200930411 . 較佳的，該界面活性劑為非離子界面活性劑。 較佳的，該非離子界面活性劑為10%聚乙烯（50)油基 醚（polyethylene (50) oleyl ether)、10% 聚乙烯（5)椰子 油脂肪酸單乙醇胺（polyethy丨ene〔 5〕coconut fatty acid monoethanol amid)或 10 %椰子油脂肪酸雙乙醇胺 (coconut fatty acid diethanol amide) ° 較佳的，該界面活性劑為陰離子界面活性劑。 較佳的，該陰離子界面活性劑為1 0%十二烷基硫酸三 p 乙醇胺（triethanolamine lauryl sulfate)、10% 十二燒基聚 氧乙婦醚硫酸納（sodium polyoxyethylene ( 4 ) lauryl ether sulfate)或10%十二烷基聚氧乙烯醚硫酸三乙醇胺 (triethanolamine polyoxyethylene ( 4 ) lauryl ether sulfate)。 較佳的，該界面活性劑為非離子界面活性劑。 較佳的，該兩性界面活性劑為1 0%十二烷基聚氧乙烯 • 鍵構酸鈉（sodium polyoxyethylene ( 10 ) lauryl ether phosphate)、10%月桂雙f基氨基乙酸甜菜素（丨aury| dimethyl aminoacetic acid betaine)、10%2-烷基-N-羧甲 基-N-經乙基-甲味嗤甜菜素（2-a丨kyl-N-carboxymethyl_N-hydroxyethyl imidazolium betain)、10%2-椰烷基-羧 f 基 -經乙基-甲咪嗅甜菜素（2-coconut alkyNN-carboxymethyl· N-hydroxyethyl imidazolium betain)或 10%2-H—基-N_ 經乙基_N-缓曱基-甲味〇坐甜菜素（2-undecy卜N-hydroxyethyl-N-carboxymethyl imidazolium betain) » 11 200930411 . 較佳的，該保濕原料進一步包含一高分子保濕原料。 較佳的’該高分子保濕原料為10%液態酵母、0.2% 透明質酸鈉（sodium hyaluronate)、10%海藻萃取物、1〇 %大豆膠原蛋白（phyto collage)、1 %海藻酸鈉溶液、〇.3 %氫化三仙膠、1%蠶絲蛋白（SILK GEN) ' 10%蠶絲蛋白 (SILK GEN)、0_2%核糖核酸鈉（RNA Sodium Salt)或 0.3 %氫化纖維素。 較佳的’該保濕原料進一步包含一高分子保濕劑。 Ό 較佳的，該高分子保濕劑為 0.5 %羧甲纖維素 (carboxymethyl cellulose)、1 0 % 聚乙烯醇（polyvinyl alcohol)、1.0% 陽離子纖維素（cationic cellulose)、1.0% 聚乙二醇 400( poly ethyl ene glycol (400) )、0.1% 羰基乙 烯基聚合物（carboxyviny丨polymer)或 1.0%聚乙二醇 1000(polyethylene glycol ( 1000))。 較佳的，該保濕原料進一步包含一高分子溶劑。 Q 較佳的，該高分子溶劑為50%異丙烯醇（isopropylene alcohol)、20% 異丙烯醇（isopropylene alcohol)、50% 乙 醇、50% 丙烯乙二醇（propy丨ene glycol)、50% 1，3-丁二醇 (1，3-butylene glycol)、50% 甘油（giycerin)或 20% 乙醇。 較佳的，進一步包含一有效載體。 較佳的，該有效載體為微脂體。 本發明又關於一種製備如上述之保濕原料的方法。 本發明亦關於一種包含如上述之黃秋葵萃取物之保濕 原料的化妝用品。 12 200930411 本發明亦關於一種包含如上述之黃秋葵萃取物之保濕 原料的皮膚保養用品。 本發明之萃取物係萃取自黃秋葵（Abe/mosc/ius esci//e/?fi/s )，其含有多醣體’具有保濕的效果，可取代 動物性之保濕原料，對於人體具有低敏感性、安全性，安 定性與有效性，可應用在相關化妝品原料中成為重要保濕 成份。 ❹ 【實施方式】 本發明中所謂之「萃取溶劑」，係用來萃取黃秋葵中 之有效活性成分’其可為極性溶液’較佳為純水、乙醇、 1，3 丁二醇（Buty丨ene G丨ycol)。 本發明中所謂之「微脂體（liposome)」，其成分為 磷脂質（磷脂酸膽鹼）（ph〇sphat丨dyl choljne, PC)，是生物 體的細胞膜主要組成之一，一般係利用微脂體（Up〇s〇me ) 〇 作為載體的技術，近來已應用在羊毛、絲織物之染色加工、 食品科技、動物飼料、民生工業、美容保養品、醫藥製劑 等。目前化妝品的製造大都以溶解、分散及乳化加工為主， 仁對於皮膚的相容性並不佳，若是能夠利用微脂粒可攜帶 物質和具有釋放功能之特性，以微脂粒作為傳送載體，則 月色增加皮膚對於化妝品組成成分的吸收，更能讓有效成分 滲透至皮廣裡，如此不但能克服化妝品不易被人體吸收的 缺點，亦能提升保養品未來的發展價值。 目前微脂體的製備依不同的形式，而具有不同的方 13 200930411 ^ 法，其中，膠過遽法、高速微射流體及旋轉蒸發法、可製 備多層微脂體；超音波法可製得單層小微脂粒；注射法、 清潔劑法、透析法及逆向揮發法可以製得單層大微脂粒 (Weiner, N., Martin, F., and Fllaz, M.s 1989, Liposomes as a drug delivery system. Drug Develop. Industrial Pharmacy. 1 5(1 0): 1523-1 554.)(Kim, H.H. and Baianu, I ·〇., 1991, Novel liposome micro encapsulation techniques for food applications. Food Sci. & Tech. ❹Otchere, G., 1977, Studies on the mucilages extracted form okra fruit (Hibiscus esculentus L.) and baobab (Adansonia L. ) . J. Sci. Food Agric. 28: 519-529.) In 1 987, Bhat and Tharanathan's adhesion to okra is 6 200930411 • A more complex composition is proposed, except for the composition of galactose, rhamnose, and galacturonic acid, which is subdivided into glucose, mannose, arabinose, and xylose. Bhat, UR and Tharanathan, RN, 1987, Fi/nci/ona/ Properties of Okra (Hibiscus esculentus) mucilage. Starch 39: 165-167.) Currently, okra is mostly edible, and the analysis of the viscous components contained in the okra has been Revealed in the aforementioned related case, however, the application of the viscous component, ❹ currently mostly for direct consumption of okra. There is no research showing that the viscous component of okra is applied to makeup or maintenance products. At present, the cosmetics that are advertised as having a moisturizing effect are mostly hyaluronic acid as a moisturizing ingredient. Hyaluronic acid, also known as hyaluronic acid or hyaluronic acid (HA), is composed of glucuronic acid-n-acetyl glucosamine. It is a linear polymer polysaccharide composed of disaccharide units. The average molecular weight is between 100,000 and 1 million Daltons (Da|t〇n), which plays an important role in skin, 'weaving and structural overall maintenance' and intercellular transport. Function; it can still be applied to cosmetics or applied to skin moisturization and wound healing (Balazs, E_A_ and Band, P., 1984, /7ya/ur〇n/c acid ../is siruciure use·, Toil, 99(6)H65 -72). The current commercial form is usually the sodium salt type, so it is called sodium hyaluronate. Hyaluronic acid is widely present in the interstitial cells of the animal tissues and in the vitreous of the eye. The physiological function is water retention and lubrication. It is a macromolecular mucopolysaccharide widely present in the connective tissue of organisms. The physiological effects of hyaluronic acid vary in different tissues. For example, the main role in the skin is moisturizing, which acts primarily as a lubricant in the joints and primarily regulates permeability in the vessel wall. In addition, 7 200930411 • The acidity of ruthenium is also a polyelectrolyte electrolyte. The large amount of negative charge on the molecule can regulate the concentration of surrounding positive and negative ions and inhibit the activity of various enzymes. Early studies have suggested that hyaluronic acid is only a kind of intercellular filling, so it has not caused much attention. In recent years, with the deep understanding of the structure, performance and bio-functionality of hyaluronic acid, hyaluronic acid has become a cell organism. Research focus in the fields of pathology and immunology. Because of its good viscoelasticity, plasticity, permeability and biocompatibility, it can be widely used in the production of clinical medicine and cosmetics. In recent years, the demand for hyaluronic acid market has increased day by day, showing good market potential and broad application prospects. Its application is divided according to the molecular weight of hyaluronic acid: small molecular weight hyaluronic acid is applied to cosmetics; large molecular weight hyaluronic acid is applied to biomedical materials. Since Europe, the United States, and Japan have had cases of mad cow disease in recent years, and based on the concept of religious non-killing and the doubt that there may be residual bacteria, the animal uses moisturizing products such as collagen, collagen, and placenta. The substance has gradually been abandoned by European and American countries, and replaced with substances that can be obtained from plants. In general, the polysaccharide component gradually replaces the original animal source products and becomes the mainstream source of moisturizing raw materials. Multi-domains gradually decrease with age in the skin. Therefore, adding an appropriate amount of polysaccharide raw materials to the skin care products can appropriately increase the skin's moisturizing function, while Taiwan and Southeast Asia are moist and hot, so make up. The practitioners all prefer moxibustion and hygroscopic moisturizing ingredients. Therefore, the selected plant αο species and their extracted components should be able to meet the conditions of refreshing, moisturizing and hygroscopicity. Easy to obtain raw materials, 200930411 - and other conditions, so how to develop 屮 ^ 1 issued the above conditions, and use the plant as a '' raw material to replace the moisturizing raw materials obtained from the animal source to improve the day:: plants in the cosmetics industry Additional value, as well as processing technology and preparation feasibility studies, are necessary for research. SUMMARY OF THE INVENTION The main object of the present invention is to provide a hydrating function of Huangqiu Caijing, the extract is in the form of a slippery substance, and the invention is vegetative, containing polysaccharides and proteins, and having The moisturizing effect can replace the animal-like moisturizing raw material and become an additive for general makeup or skin care products. 'The invention has been tested and proved to have no allergic reaction to human skin', and has good safety and moisturizing effectiveness. In order to achieve the above object, the present invention provides an extraction method of an okra extract having a moisturizing function, which is prepared by the following steps: obtaining okra and an extraction solution; & extracting okra and extracting solvent by i: i~丄The ratio of i: 混合 is mixed with 1 - 1 0 0 °c for extraction reaction; and the initial extract solution is collected and filtered to obtain a moisturizing okra extract. Preferably, the okra extract has a multi-cool body. Preferably, the okra extract has a protein. Preferably, the obtained okra still includes washing, peeling and chopping. Preferably, the extraction solvent is a polar solution. Preferably, the extraction solvent is pure water, ethanol or 1,3 butanediol. 9 200930411 Preferably, the extraction solvent is preferred, and the draw solvent is preferably 0% by volume of 1,3 butyl alcohol. 1 0 0 % by volume of pure water. 1 0 0% by volume of ethanol. 60% by volume of pure water, 2 alcohol, and 20% by volume of 6 preferably, the extraction solvent is 80% by volume of pure water, and 20% by volume of 1,3 butanediol. Preferably, the extraction solvent is 80% by volume of pure water and 20% by volume of ethanol β, preferably 8 Å. (: The extraction solvent is configured. Preferably, the extraction solution is configured at 1 〇 0 ° C. Preferably, the mixing ratio of the okra and the extraction solvent is 1:10 ° preferably 'the mixture of the okra and the extraction solvent The ratio is 1:10. Preferably, the okra and the extraction solvent are mixed and subjected to a backlash extraction. Preferably, the okra and the extraction solvent are mixed with a reduced pressure rotary concentrator: EYELA, SB. Preferably, an effective carrier is further used. Preferably, the carrier is a liposome. The present invention relates to a moisturizing raw material of the @秋葵 extract extracted by the above extraction method. Preferably, the moisturizing material further comprises an interface active engraving 10 200930411. Preferably, the surfactant is a nonionic surfactant. Preferably, the nonionic surfactant is 10% polyethylene (50) oil based. Ether (polyethylene (50) oleyl ether), 10% polyethylene (5) coconut oil fatty acid monoethanolamine (polyethy丨ene [5] coconut fatty acid monoethanol amid) or 10% coconut oil fatty acid diethanolamine (coconut fatty acid diethanol amide) ° Preferably, the surfactant is an anionic surfactant. Preferably, the anionic surfactant is 10% triethanolamine lauryl sulfate, 10 % sodium polyoxyethylene (4) lauryl ether sulfate or 10% triethanolamine polyoxyethylene (4) lauryl ether sulfate. The surfactant is a nonionic surfactant. Preferably, the amphoteric surfactant is 10% sodium polyoxyethylene (10) lauryl ether phosphate, 10% Dimethylaury| dimethyl aminoacetic acid betaine, 10% 2-alkyl-N-carboxymethyl-N-ethyl-methyl miso betaine (2-a丨kyl-N) -carboxymethyl_N-hydroxyethyl imidazolium betain), 10% 2-cocoalkyl-carboxyf-yl- 2-coconut alkyNN-carboxymethyl· N-hydroxyethyl imidazolium betain or 10% 2-H- base-N_ via ethyl_N- slow Preferably, the moisturizing raw material further comprises a macromolecular moisturizing raw material. Preferably, the polymer moisturizing raw material is 10% liquid yeast, 0.2% sodium hyaluronate, 10% seaweed extract, 1% phyto collage, 1% sodium alginate solution, 3. 3 % hydrogenated trisin, 1% silk protein (SILK GEN) '10% silk protein (SILK GEN), 0_2% sodium ribonucleotide (RNA Sodium Salt) or 0.3% hydrogenated cellulose. Preferably, the moisturizing material further comprises a polymeric humectant. Preferably, the polymeric humectant is 0.5% carboxymethyl cellulose, 10% polyvinyl alcohol, 1.0% cationic cellulose, 1.0% polyethylene glycol 400 (poly ethyl ene glycol (400)), 0.1% carboxyviny® polymer or 1.0% polyethylene glycol (1000). Preferably, the moisturizing raw material further comprises a polymer solvent. Preferably, the polymer solvent is 50% isopropylene alcohol, 20% isopropylene alcohol, 50% ethanol, 50% propy丨ene glycol, 50% 1 , 1,3-butylene glycol, 50% giycerin or 20% ethanol. Preferably, an effective carrier is further included. Preferably, the effective carrier is a liposome. The invention further relates to a method of preparing a moisturizing material as described above. The present invention also relates to a cosmetic comprising a moisturizing raw material of the okra extract as described above. 12 200930411 The present invention also relates to a skin care product comprising a moisturizing raw material of the okra extract as described above. The extract of the present invention is extracted from okra (Abe/mosc/ius esci//e/?fi/s), which contains a polysaccharide having a moisturizing effect, can replace an animal moisturizing raw material, and has low sensitivity to the human body. , safety, stability and effectiveness, can be applied to the relevant cosmetic raw materials to become an important moisturizing ingredient.实施 [Embodiment] The so-called "extraction solvent" in the present invention is used for extracting an effective active ingredient in okra 'which may be a polar solution', preferably pure water, ethanol, 1,3 butanediol (Buty丨ene) G丨ycol). The "liposome" in the present invention is a phospholipid (phosphocholine chojne, PC), which is one of the main components of a cell membrane of a living body, and is generally used in microfabrication. The technology of the fat body (Up〇s〇me) 〇 has recently been applied to the dyeing processing of wool and silk fabrics, food technology, animal feed, people's livelihood industry, beauty care products, pharmaceutical preparations and the like. At present, most of the cosmetics are mainly processed by dissolving, dispersing and emulsifying. The compatibility of the kernel with the skin is not good. If the liposome can be used as a carrier and has a releasing function, the microlipid is used as a transport carrier. The moon color increases the absorption of the cosmetic components of the skin, and allows the active ingredients to penetrate into the skin. This not only overcomes the shortcomings of the cosmetics being easily absorbed by the human body, but also enhances the future development value of the skin care products. At present, the preparation of the liposome is different according to different forms, and has different methods. The method can be prepared by a method of ultrasonic permeation, high-speed micro-jet fluid and rotary evaporation, and a multi-layered liposome can be prepared; Single layer of small vesicles; injection, detergent, dialysis and reverse volatilization can produce a single layer of large vesicles (Weiner, N., Martin, F., and Fllaz, Ms 1989, Liposomes as a drug) Drug development. Industrial Pharmacy. 1 5(1 0): 1523-1 554.) (Kim, HH and Baianu, I · 〇., 1991, Novel liposome micro encapsulation techniques for food applications. Food Sci. & Tech. ❹
Mar:55-61.)(Jackson, L. S., Lee, K.,1991,Mar: 55-61.) (Jackson, L. S., Lee, K., 1991,
Microencapsulation and the food industry. Lebensm. Wiss. U. Technol., 24:289.) (Zhang, L., HU, J., LU, Z., 1997, Preparation of liposomes with a controlled assembly procedure. J.Colloid Interface Sci. 190:76-80) 〇 實施例 實施例一：萃取黃秋葵成份之條件與方法 本發明所使用萃取溶劑為純水、乙醇、1,3 丁二醇 (Buty丨ene G丨yco丨，BG)三種物質，依比例及温度分成10 組，其中配方A為溫度設定為80°C，配方B為溫度設定 為 10CTC。 一、A-01配方為80°C，B-01配方為10CTC，皆以純 水為溶劑以萃取黄秋葵。 二、A-02配方為80°C，B-01配方為100°C，皆以乙 200930411 醇為溶劑以萃取黃秋葵。 三、 A-03配方為80°C，B-01配方為100°C，皆以純 水 60%、1，3 丁二醇（Butylene Glycol, BG) 20%、乙醇 20 %混合比例以萃取黃秋葵。 四、 A-04配方為80°C，B-01配方為100°C，皆以80 % 純水、20% 1,3 丁二醇（Butylene Glyco丨，1,3BG)為混合 比例以萃取黃秋葵。 五、 A-05配方為80°C，B-01配方為100°C，皆以80 %純水、20%乙醇為混合比例以萃取黄秋葵。 依上述比例調配成為萃取溶劑使用，表一顯示在80°C 及1 00°C之配方溶劑比例，其中黃秋葵與溶劑混合比例分 別為1 : 10。Microbenstion and the food industry. Lebensm. Wiss. U. Technol., 24:289.) (Zhang, L., HU, J., LU, Z., 1997, Preparation of liposomes with a controlled assembly procedure. J. Colloid Interface Sci. 190:76-80) EXAMPLES Example 1: Conditions and Methods for Extracting Okra Components The extraction solvent used in the present invention is pure water, ethanol, and 1,3 butanediol (Buty丨ene G丨yco®, BG) The three substances are divided into 10 groups according to the ratio and temperature, wherein the formula A is set to a temperature of 80 ° C, and the formula B is set to a temperature of 10 CTC. 1. The A-01 formula is 80 ° C, and the B-01 formula is 10 CTC. The pure water is used as a solvent to extract the okra. Second, the A-02 formula is 80 ° C, and the B-01 formula is 100 ° C. Both of them use the 200930411 alcohol as a solvent to extract the okra. Third, A-03 formula is 80 ° C, B-01 formula is 100 ° C, all with pure water 60%, 1,3 butanediol (Butylene Glycol, BG) 20%, ethanol 20% mixing ratio to extract okra . 4. The A-04 formula is 80 ° C, and the B-01 formula is 100 ° C. Both are 80% pure water and 20% 1,3 butanediol (Butylene Glyco®, 1,3BG) as the mixing ratio to extract the okra. . 5. The A-05 formula is 80 ° C, and the B-01 formula is 100 ° C. Both are 80% pure water and 20% ethanol in a mixture ratio to extract okra. According to the above ratio, it is used as an extraction solvent. Table 1 shows the ratio of the formulation solvent at 80 ° C and 100 ° C, wherein the mixing ratio of okra and solvent is 1:10.
表一 ：80°C及1 〇〇°C之各配方溶劑比例 溶劑及比例（重量比） 配方 純水 1.3BG 乙醇 A-01 100% A-02 100% A-03 60% 20% 20% A-04 80% 20% A-05 80% 20% B-01 100% B-02 100% B-03 60% 20% 20% B-04 80% 20% B-05 80% 20% 15 200930411 將黄秋葵進行前處理，其中前處理包含清洗、去皮及 碾碎；將黃秋葵和上述各組溶劑混合備用；進行迴旋濃縮萃 取（減壓迴旋濃縮機：EYELA，SB-1 000 );收集初萃物； 將前述初萃物過慮；獲得本發明之黃秋葵萃取物。 結果 於10CTC萃取條件下的5個不同溶劑組所萃取出的黃Table 1: Solvent ratios and ratios (weight ratio) of each formulation solvent at 80 ° C and 1 ° ° C Formulation pure water 1.3 BG Ethanol A-01 100% A-02 100% A-03 60% 20% 20% A -04 80% 20% A-05 80% 20% B-01 100% B-02 100% B-03 60% 20% 20% B-04 80% 20% B-05 80% 20% 15 200930411 Will Okra Pre-treatment, wherein the pre-treatment comprises washing, peeling and milling; mixing the okra with the above respective groups of solvents; performing concentrated convolution extraction (pressure deceleration concentrator: EYELA, SB-1 000); collecting the initial extract; The foregoing preliminary extract is overtreated; the okra extract of the present invention is obtained. Results Yellow extracted from 5 different solvent groups under 10CTC extraction conditions
秋葵萃取物及8(TC：萃取條件下的5個不同溶劑組所萃取之 黃秋葵萃取物，在顏色外觀上有明顯不同10(TC萃取條件 顏色較深，8(TC萃取條件顏色較淺，但放置隔天即同樣有 沉殿產生，經升温後即回復透明狀，推測可能温度較高時 溶解度較佳冷却回温後過飽和的原因造成，不同溶劑組、 萃取的黏質成份外觀也完全不同，因此萃取溫度不同、砝 果也呈現很大差異’所以温度、萃取時間、萃取次數心 劑的不同及溶劑混合比例的差異、都對萃取物質之 很大影響。 百Okra extract and 8 (TC: extract of okra extract from 5 different solvent groups under extraction conditions, the color appearance is significantly different 10 (TC extraction conditions are darker, 8 (TC extraction conditions are lighter, However, the next day, there is also a sinking hall. After warming up, it will return to the transparent shape. It is speculated that the solubility may be better when the temperature is higher, and the supersaturation after cooling and warming may cause the different solvent groups and the extracted viscosity components to be completely different. Therefore, the extraction temperature is different, and the capsules also show great differences. Therefore, the temperature, the extraction time, the difference in the number of extractions, and the difference in the solvent mixing ratio all have a great influence on the extracted substances.
實施例二：黃秋葵萃取成份之分析 1.萃取率測量方法 以100 g黃秋葵加1000 g 後用遽紙遽除雜質及不溶部份 後，將漠縮液置於冷凍乾燥機 末。 (〇倍）溶液抽取，5小時 丄濾液以減壓蒸餾去除溶劑 進行凌乾，以獲得乾燥粉 萃取率計算公式： 萃取率=〔W 1/W2〕χ100% 16 200930411 • w 1 :黃秋葵原重量 W2:W1萃取凍乾後所得乾燥粉末重量 2·黃秋蔡之成份分析（AO AC; 1 984 )之方法測定： 水分 C Moisture ) 取黃秋葵100g放入已達恆重的坩堝中，置於1〇5。(3恆 0 温乾燥箱中’每小時取出。放在乾燥器中冷卻30分鐘後 稱重，直到恆重為止》 水分計算公式： 水分（％ ) =〔（ W1-W2) | ( W 1 -W0)〕χ1〇〇% W0 :坩堝恆重量（g ) W1 :坩堝重十樣本重量（g〉 W2 : W1乾燥至恆重的重量（g〉 ❹ 灰分（Ash ) 取貫秋凑100g放入已達恒重的掛禍中，置入55〇。〇 的灰化爐中’灰化6小時以上取出放入乾燥器中冷卻，稱 重直到恆重為止。 灰分計算公式： 灰分 ％= ( W1-W0/S) x100% W0 :坩堝恆重量（g ) W1:掛禍重十燃燒後樣本重量（g) S : W樣品的重量（g ) 17 200930411 ^水份及灰份的百分比 將1〇〇g黄秋葵置於1〇5。(：恆溫乾燥箱中至恆重時剩 下7.25g，100g黃秋葵置於550。(：灰化爐中6小時後稱 重剩下0_85g，黃秋葵l〇〇g，其水份及低揮發物質佔925 % ’灰份為0.8%。 本發明之黄秋葵萃取率測試黃秋葵在萃取條件不 同、時間、溫度和不相同溶劑及不同比例混合的溶劑，所 萃取出來的黄秋葵黏質成份的百分比。8CTC、1 00。〇中之 A-03、B-03及A-04、B-04因含1,3BG、揮發點及凝固點都比水 較高、所以改用恆温烘箱乾燥處理，因為一般冷凍乾燥機 無法達成。 表二：於不同溫度（8〇 t：及100。〇以不同溶劑萃取4小時之萃取率 配方 ------ 萃取率 A-01 1% A-02 8% A-03 6% A-04 5% A-05 . ------- 6% B-01 8% B-02 9% B-03 6% B-04 596 B-05 796 18 200930411 • 由表二可看出在1〇〇°C下所萃取之黄秋葵萃取率比以 80 C萃取之黄秋葵萃取率高’就外觀上來看抽取液 顏色也比80°C深’很明顯可以觀察到溶劑及温度的不同， 對秋葵萃取成份也有明顯差異，乙醇、純水等單一溶劑之 抽取比率比混合溶劑之抽取率好，尤其是乙醇抽取率比其 他溶劑高。 _ 3.多酷體含量測定 Ο 依據 Dubois 等人的方法（pheno- sulfuric method) 以半乳糖作為標準品（Dubois M，Gilles KA，Hamilton JK.Revers PA,Smith F.,1956,Colorimetric method for determination of sugars and related substances.AnalExample 2: Analysis of extract components of okra 1. Method for measuring extraction rate After removing 100 g of okra and 1000 g and removing impurities and insoluble portions with a crepe paper, the desert liquid was placed at the end of the freeze dryer. (〇倍) solution extraction, 5 hours of 丄 filtrate filtrate distillation under reduced pressure for drying, to obtain the dry powder extraction rate calculation formula: extraction rate = [W 1 / W2] χ 100% 16 200930411 • w 1 : original weight of okra W2: W1 extraction dry powder weight obtained after lyophilization 2. Determination of the composition of Huangqiu Cai (AO AC; 1 984): Moisture C Moisture) Take 100g of okra into the sputum that has reached constant weight and put it in 1 〇 5. (3 constant 0 temperature drying oven 'take it out every hour. Put it in the dryer for 30 minutes and then weigh it until constant weight.> Moisture calculation formula: Moisture (%) = [( W1-W2) | ( W 1 - W0)〕χ1〇〇% W0 :坩埚 constant weight (g) W1: 坩埚 weight ten sample weight (g> W2: W1 dry to constant weight weight (g> ❹ ash (Ash) In the smashing of the constant weight, put 55 〇. In the ashing furnace of the ' ashing for more than 6 hours, take it out and put it in a desiccator to cool it, and weigh it until it is constant weight. Formula of ash: ash %= ( W1- W0/S) x100% W0 : constant weight (g) W1: weight of the sample after burnt weight (g) S : weight of sample W (g) 17 200930411 ^The percentage of moisture and ash will be 1〇〇 g Okra is placed at 1〇5. (: 7.25g is left in the constant temperature drying oven to constant weight, and 100g okra is placed in 550. (: After 6 hours in the ashing furnace, the remaining 0_85g, okra l〇〇g, Its water content and low volatile matter accounted for 925 % 'ash content of 0.8%. The okra extraction rate of the present invention is tested by different okra extraction conditions, time, temperature and different solvents and The percentage of the components of the okra that are extracted in different proportions. 8CTC, 100. A-03, B-03 and A-04, B-04 in the sputum contain 1,3BG, volatilization point and freezing point. They are all higher than water, so they are changed to a constant temperature oven for drying, because the general freeze dryer can not be achieved. Table 2: At different temperatures (8〇t: and 100. 萃取 extraction with different solvents for 4 hours extraction rate formula --- --- Extraction rate A-01 1% A-02 8% A-03 6% A-04 5% A-05 . ------- 6% B-01 8% B-02 9% B- 03 6% B-04 596 B-05 796 18 200930411 • It can be seen from Table 2 that the extraction rate of okra extracted at 1 °C is higher than that of okra extracted with 80 C. The color is also deeper than 80 °C. It is obvious that the difference in solvent and temperature can be observed. The extraction composition of okra is also significantly different. The extraction ratio of single solvent such as ethanol and pure water is better than that of mixed solvent, especially ethanol extraction. The rate is higher than other solvents. _ 3. Determination of multi-cool content Ο According to the method of Dubois et al. (pheno-sulfonic method), galactose is used as a standard (Dub Ois M, Gilles KA, Hamilton JK. Revers PA, Smith F., 1956, Colorimetric method for determination of sugars and related substances.
Chem.28:350-356·) 〇 配製 〇 mg/L、12.5 mg/L、25 mg/L、 50 mg/L、7 mg/L·、1 〇〇 mg/L、等已知濃度半乳糖標準溶 液’利用標準溶液的濃度和吸光值的關係製成迴歸曲線， Q 再以迴歸曲線將待測溶液的吸光值’換算成相對應的濃 度，即可求得樣品多糖體含量。 首先配製黃秋葵抽憨溶液。取1 m丨黃秋葵抽取溶液加 入1 m丨的5%盼（phenol solution)，再直接沖入5 ml的濃 硫酸’冷卻30分鐘後震盪混合均勻，利用分光光度計 (HITACH U1800 spectrpophotometer)於 490 nm 測 其吸光值’並做標準曲線計算其濃度。 結果 如第二圖所示，多醣體萃取率反而是純水/1,3BG /乙 200930411 .醇，純水/1，3BG等混合之溶劑抽取率比較高，其他溶劑反 而變得較低，在100°c條件或8(rc條件了都同樣有此現象。 4.蛋白質含量測定 本實驗以 Bradford pr〇teilvbinding assay 的方法進行 蛋白質定量測定。此方法是利用c〇omass丨s Br•丨丨liant B|ue G-250會與蛋白質結合的持性。並且G 25〇與蛋白質結合 後，G-250的顏色會從紅色轉變成藍色。而且在595 nm ❹波長下會有很高吸收值。此方法方便而靈敏，其優點為g_ 250與蛋白質結合的時間較短（大約2分鐘左右），且結 合的G-250蛋白質複合物（c〇mp丨ex)可在溶液中維持較 長時間（大約1小時）。 試谢S己掣 牛血清蛋白（bovine serum a丨bumin，BSA)。將牛血 '月蛋白標準品配製成〇.125 mg/m卜0.250 mg/rrM、0.500 叫/ml、0.750 mg/mh 1〇〇〇 mg/m| 5 種濃度的標準液。 © 蛋白質含量測定 取〇·1 m丨樣品放於測試管中，加入3 m丨試劑，在波長 ηητι下測定吸光值。標準液之測定步驟與樣品步驟相 同，利用標準溶液的濃度和吸光值的關係製成迴歸曲線， 再以迴歸曲線將待測溶液的吸光值，換算成相對應濃度求 传樣品蛋白質的含量。 如第三圖所示’蛋白質萃取率在8(rc時乙醇萃取率最 200930411 高，在1 00°C時反而變小，因為乙醇揮發度較底在80°c以 * 下的關係，另外本發明的蛋白質萃取率還是以（純水/1,3BG / 乙醇）之混合溶劑萃取所獲得知蛋白質比率較高，其他溶劑 反而變得比較低，根據上述結果將黃秋葵萃取物所含之多 醣體及蛋白質成分含量整理於下表三。Chem.28:350-356·) Preparation of 半gal/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 7 mg/L·, 1 〇〇mg/L, etc. The standard solution 'uses the relationship between the concentration of the standard solution and the absorbance value to make a regression curve, and Q converts the absorbance value of the solution to be measured into a corresponding concentration by a regression curve, and the sample polysaccharide content can be obtained. First, prepare a yellow okra pumping solution. Take 1 m 丨 秋 okra extract solution and add 1 m 丨 5% phenol solution, then directly into 5 ml of concentrated sulphuric acid'. After cooling for 30 minutes, shake and mix well. Use HITACH U1800 spectrpophotometer at 490 nm. Measure its absorbance value and calculate the concentration by making a standard curve. As shown in the second figure, the extraction rate of polysaccharides is pure water/1,3BG/B 200930411. The solvent extraction rate of alcohol, pure water/1,3BG, etc. is relatively high, and other solvents become lower. This is also the case for 100°c conditions or 8 (rc conditions. 4. Protein content determination This experiment uses the Bradford pr〇teilvbinding assay for protein quantification. This method uses c〇omass丨s Br•丨丨liant B|ue G-250 binds to proteins. When G 25〇 binds to proteins, the color of G-250 changes from red to blue, and it has a high absorption at 595 nm. This method is convenient and sensitive, and has the advantage that g_250 binds to the protein for a short period of time (about 2 minutes), and the combined G-250 protein complex (c〇mp丨ex) can be maintained in solution for a long time ( About 1 hour) Thanks to bovine serum a丨bumin (BSA). The bovine blood 'month protein standard is formulated into 125.125 mg/m b 0.250 mg/rrM, 0.500 call/ml , 0.750 mg/mh 1〇〇〇mg/m| 5 concentrations of standard solution © Protein Measure the sample of 〇·1 m丨 and place it in the test tube, add 3 m 丨 reagent, and measure the absorbance at the wavelength ηητι. The measurement procedure of the standard solution is the same as the sample step, and the relationship between the concentration of the standard solution and the absorbance is used. In the regression curve, the absorbance of the solution to be tested is converted into the corresponding concentration to obtain the content of the sample protein by the regression curve. As shown in the third figure, the protein extraction rate is 8 (the ethanol extraction rate is the highest at 200930411, It is smaller at 100 ° C because the volatility of ethanol is lower than 80 ° C in the bottom, and the protein extraction rate of the present invention is still extracted with a mixed solvent of (pure water / 1,3 BG / ethanol). The obtained protein ratio was higher, and the other solvents became lower. According to the above results, the content of the polysaccharide and protein components contained in the okra extract was summarized in Table 3 below.
表三：黄秋葵萃取物之多醣體及蛋白質成份百分比 配方 多醣體 蛋白質 A-01 16.750% 0.134% A-02 16.601% 0.492% A-03 19.503% 0.248% A-04 19.984% 0.126% A-05 16.601% 0.196% B-01 16.912% 0.232% B-02 16.601% 0.168% B-03 19.984% 0.376% B-04 19.984% 0.216% B-05 16.912% 0.170% 實施例三：黃秋葵萃取成份之安定性與化妝品原料融合 性測試 1.時效安定性 將黃秋葵萃取液分別置於4°c、常溫下、窗戶邊、37°c 21 200930411 •下和50°C環境下，觀察沉澱物、味道_和顏色變化，觀察期 時間為28天，其中100°C萃取物質及8(TC萃取物質調配 成5%萃取液。 結果 味道並沒有改變或產生變化，只有乙醇萃取部份顏色 由草綠色變為較淺色，可能是乙醇萃取出的葉綠素成份受 光線、温度等影響所致，其餘萃取液都是安定的沒有產生 & 變化。Table 3: Percentage of polysaccharides and protein components of okra extract Formulation polysaccharide protein A-01 16.750% 0.134% A-02 16.601% 0.492% A-03 19.503% 0.248% A-04 19.984% 0.126% A-05 16.601% 0.196% B-01 16.912% 0.232% B-02 16.601% 0.168% B-03 19.984% 0.376% B-04 19.984% 0.216% B-05 16.912% 0.170% Example 3: Stability of okra extract ingredients and cosmetic raw materials Fusion test 1. Ageing stability The okra extract was placed at 4 ° C, room temperature, window side, 37 ° c 21 200930411 • under and 50 ° C environment, observe the sediment, taste _ and color change, observe The period is 28 days, of which 100 ° C extract and 8 (TC extract is formulated into 5% extract. The taste does not change or change, only the ethanol extract part of the color changes from grass green to lighter color, possible The chlorophyll component extracted by ethanol is affected by light, temperature, etc., and the other extracts are stable and do not produce & changes.
IlPH安定性測試 使用乳酸及NaOH來調整黃秋葵萃取物pH值，調整 範圍PH值2 - 9，觀察沉澱物、味道和顏色變化，觀察期 時間28天。 調整5%黃秋葵萃取物之pH值，調整範圍pH值為2 〜9，驗性中pH值增加，其顏色比率也會相對加深；在酸 丨性範圍内顏色並沒有此現象產生。但是在較低酸性範圍下 含有乙醇溶劑之萃取物質會有混濁情形產生，再將ρΗ值 調高至pH值4以上後就變回透明無混濁現象，因此可以 判斷萃取液在PH4〜8範圍内是穩定的。 I熬安定性 將黃秋葵萃取液加熱至85°C時持溫8小時，觀察其外 觀變化’並測量加熱後的黏度及再回復常溫時的耗产。 結果：將黃秋葵萃取液加熱85〇C持續8小時，觀察到 的點度、沉澱狀態、味道、顏色等都沒有變化，證實黃秋 22 200930411 葵萃取液對熱呈現安定性。 所萃及1帆萃取條件下的1G個不同溶劑組， :卒取黃秋葵的萃取物，將其配製成含有㈣黃秋葵萃取 物的浴液’其係分別與10%非離子界面活性劑、卿陰離 子界面活性劑和10%兩性界面活性劑混合，確認黃秋蔡黏IlPH stability test Use lactic acid and NaOH to adjust the pH of the okra extract, adjust the range of pH 2 - 9, observe the changes in sediment, taste and color, the observation period is 28 days. Adjust the pH value of 5% okra extract, adjust the pH value to 2~9, increase the pH value during the test, and the color ratio will be relatively deeper; the color does not occur in the range of acidity. However, in the lower acidic range, the extracting substance containing the ethanol solvent may be turbid, and after the pH value is increased to pH 4 or higher, it will return to a transparent and turbid state, so that the extract can be judged to be in the range of pH 4 to 8. It is stable. I熬 Stability When the okra extract was heated to 85 ° C for 8 hours, the appearance change was observed and the viscosity after heating was measured and the consumption was resumed at normal temperature. RESULTS: The okra extract was heated at 85 ° C for 8 hours. The observed spots, precipitation state, taste, color, etc. did not change. It was confirmed that Huang Qiu 22 200930411 The sunflower extract showed stability to heat. 1G different solvent groups under extraction and 1 sail extraction conditions: Stretching okra extract, which is formulated into a bath containing (iv) okra extract, which is separately with 10% nonionic surfactant, Anionic surfactant and 10% amphoteric surfactant are mixed to confirm the yellow autumn clay stickiness
物貝疋否適合作為各界面活性劑中之保濕劑。觀察其相溶 性’其觀察時間28天。 結果 黃秋葵萃取溶液與非離子界面活性劑、陰離子界面活 I1 生J兩性界面活性劑混合，其相融性良好沒有混濁情形 產生，因此確認黄秋葵黏物質是適合在界面活性劑中當保 濕劑使用，下列4-1〜4-3為所使用之各種活性界面活性劑。 11:非離手界面活性劑： a. 10% 聚乙烯（50)油基謎（p0|yethy|ene ( 50) oleyl ether) b. 10%聚乙烯（5〉椰子油脂肪酸單乙醇胺（p0|yethyiene (5〕coconut fatty acid monoethanol amide) c-1〇%椰子油脂肪酸雙乙酵胺（coconut fatty acid diethanol amide) 4-2:陰籬孚界面活性劑： a_10%十二炫基硫酸三乙醇胺（tri ethanol amine丨auryl sulfate) b_1〇 %十二炫基聚氧乙稀驗硫酸納（sodium 23 200930411 • polyoxyethylene ( 4) lauryl ether sulfate) c. 10%十二烷基聚氧乙烯醚硫酸三乙醇胺 (triethanolamine polyoxyethylene( 4)lauryl ether sulfate) 4-3:雨性界面活性劑： a. 10 %十二烧基聚氧乙歸趟碟酸納（sodium polyoxyethylene ( 10) lauryl ether phosphate) b. 10 % 月桂雙甲基氨基乙酸甜菜素（丨aury I dim ethyl aminoacetic acid betaine) e c. 10% 2-烷基-N-羧曱基-N·羥乙基-曱咪唑甜菜素（2- a Iky 卜N-carboxymethy 卜N-hydroxy ethyl imidazolium betain) d_10% 2-椰烷基-羧曱基-羥乙基-曱咪唑甜菜素（2> coconut alkyl-N-carboxymethyl-N-hydroxyethyl imidazolium betain) e.10% 2--J--基-N-羧乙基-N-緩甲基-甲咪唾甜菜素 ❹ （LundecyrN-hydroxyethy卜N-carboxymethyl imidazolium betain) 5·與化妝品原料的融合性 80°C及1 00°C萃取條件下的1 0個不同溶劑組所萃取黃 秋葵的萃取物’配製成5%溶液與化妝品高分子保濕原料 混合’觀察其相溶性，觀察時間28天。 結果 黃秋葵溶液與化妝品高分子保濕劑混合其相溶性是穩 定的沒有混濁情形，在50%乙醇下只有A~02組以乙醇為 24 200930411 溶劑所萃取溶液相融合無變化外，其他以水、1，3bg為溶 劑所萃取的物質’則在50%乙醇下形成混濁，但在2〇% 乙醇下並沒有產生混濁情形，可能是親水性物質在高濃度 極性物質下所產生之反應變化。確認黃秋葵溶液與化妝品 高分子保濕劑混合之相溶性是穩定的，只要避免與高濃声 極性溶劑混合，則能適合應用在化妝品原料中當保濕劑使 用。 本發明所使用的化妝品高分孑保濕原斜： 3.10% 液態酵母 b.0.2% 透明質酸鈉（sodium hyaluronate) 海藻萃取物 d. 10% 大豆谬原蛋白（phyto collage) e. 196海藻酸鈉溶液 ί_〇_3%氫化三仙膠 g. 1% 蠶絲蛋白（SILK GEN) h. 10% 蠶絲蛋白（SILK GEN) i. 0.2% 核糖核酸鈉（rna Sodium Salt) j. 0.3%氫化纖維素 取於80°C及10(TC萃取條件下的10個不同溶劑組所 萃取之黃秋葵萃取物，取5%黃秋葵萃取物溶液與下列各 化妝品高分子保濕劑及溶劑混合，並觀察其相溶性，觀察 時間28天。 座AM所使用的_化妝品高分子徭湛劑： 25 200930411 • a·0.5% 幾甲纖維素（carboxymethyl cellulose) b.1_〇% 聚乙稀醇（polyvinyl alcohol) c· 1.0% 陽離子纖維素（cationic cellulose) d. 1 ·0% 聚乙二醇 400(polyethylene glycol ( 400 )) e. 0.1% 幾基乙稀基聚合物（carboxy vinyl polymer) 聚乙二醇 1000(p〇|yethyleneglycol ( 1000)) 溶劑 ❹ a. 50% 異丙稀醇（isopropyl ene alcohol) b. 20% 異丙烯醇（iS〇pr〇py|ene aic〇h〇|) c. 50%乙醇 d. 50% 丙婦乙二醇（pr〇py|ene g|yC〇|) e. 50%1,3-丁二醇（1,3_buty|ene g|ycol) f. 50% 甘油（giycerjn) 9.20%乙醇 結果 © 黄秋葵溶液與化妝品高分子保濕原料混合其相容性是 女疋的’沒有混濁情形產生，故能確認黃秋葵黏物質是適 合在化妝品原料中當保濕劑使用。 實施例四：黃秋葵成份對皮膚之安全測試 過敏測試 將黃秋葵萃取黏質成份帆不同溶劑組與1〇〇£〇不同 洛劑組，包覆於微脂粒中，並製成過敏貼布，並以分別在 C,、1〇GC中所萃取之黃秋葵萃取黏質成份而未包覆於 26 200930411 - 微脂粒中所製成的過敏貼布作為對照組，分別貼在20個 自願接受測試人員的上臂内，24小時後移除，觀察受測者 疋否有產生皮膚刺激感或過敏現象，根據皮膚反應評價的 評分法、並記錄其結果，以皮膚反應的評價來作為得分表 的分數。（Draize,F·，Woodard, G.，Calvery，H. 0” 1948Whether it is suitable as a moisturizer in each surfactant. The compatibility was observed and its observation time was 28 days. Results The okra extract solution was mixed with nonionic surfactant and anionic interfacial I1 biosynthesis surfactant. The phase fusion was good and there was no turbidity. Therefore, it was confirmed that the okra mucoid is suitable for use as a moisturizer in the surfactant. The following 4-1 to 4-3 are various active surfactants used. 11: Non-hand-off surfactant: a. 10% polyethylene (50) oil-based mystery (p0|yethy|ene (50) oleyl ether) b. 10% polyethylene (5> coconut oil fatty acid monoethanolamine (p0| Yethyiene (5)coconut fatty acid monoethanol amide) c-1〇% coconut fatty acid diethanol amide 4-2: yinfu surfactant: a_10% tauthyltriethanolamine Triethanol amine丨auryl sulfate) b_1〇%12 succinyl polyoxyethylene sulfate sodium sulfate (sodium 23 200930411 • polyoxyethylene (4) lauryl ether sulfate) c. 10% dodecyl polyoxyethylene ether triethanolamine sulfate ( Triethanolamine polyoxyethylene(4)lauryl ether sulfate) 4-3: Rainy surfactant: a. 10% sodium polyoxyethylene (10) lauryl ether phosphate b. 10 % laurel丨aury I dim ethyl aminoacetic acid betaine e c. 10% 2-alkyl-N-carboxymethyl-N-hydroxyethyl-imidazole betain (2- a Iky Bu N -carboxymethy 卜 N-hydroxy ethyl imidazolium betain) d_10% 2-cocoalkyl-carboxyl-hydroxyl -2-glycolic acid-N-carboxymethyl-N-hydroxyethyl imidazolium betain e.10% 2--J--yl-N-carboxyethyl-N-hypothymomethyl-methicin L (Lundecyr N-carboxyethy, N-carboxymethyl imidazolium betain) 5. Condensation with cosmetic raw materials Extract of okra extracted in 10 different solvent groups at 80 ° C and 100 ° C extraction conditions 5% The solution was mixed with the cosmetic polymer moisturizing raw material to observe the compatibility. The observation time was 28 days. Results The compatibility of the okra solution with the cosmetic polymer moisturizer was stable and there was no turbidity. Under the 50% ethanol, only the A~02 group was used. Ethanol is 24 200930411 The solvent extracted by the solvent does not change, and other substances extracted with water and 1,3 bg as solvent are turbid under 50% ethanol, but no turbidity occurs under 2% ethanol. It may be a change in the reaction of a hydrophilic substance under a high concentration of a polar substance. It is confirmed that the compatibility of the okra solution and the cosmetic polymer humectant is stable, and it can be suitably used as a humectant in a cosmetic raw material as long as it is prevented from being mixed with a high-concentration polar solvent. The high-yield moisturizing original used in the present invention: 3.10% liquid yeast b.0.2% sodium hyaluronate seaweed extract d. 10% soybean prion protein (phyto collage) e. 196 sodium alginate Solution ί_〇_3% hydrogenated Sanxian G. 1% silk protein (SILK GEN) h. 10% silk protein (SILK GEN) i. 0.2% sodium riboate (rna Sodium Salt) j. 0.3% hydrogenated cellulose Take the okra extract extracted from 80 different solvent groups at 80 ° C and 10 (TC extraction conditions), and mix 5% okra extract solution with the following cosmetic macromolecular humectants and solvents, and observe the compatibility. Observation time 28 days. _ Cosmetic polymer 徭 徭 : 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 % cationic cellulose d. 1 · 0% polyethylene glycol 400 (polyethylene glycol (400 )) e. 0.1% carboxy vinyl polymer polyethylene glycol 1000 (p〇 |yethyleneglycol ( 1000)) Solvent ❹ a. 50% isopropyl ene Alcohol) b. 20% isopropenol (iS〇pr〇py|ene aic〇h〇|) c. 50% ethanol d. 50% propylene glycol (pr〇py|ene g|yC〇|) e 50% 1,3-butanediol (1,3_buty|ene g|ycol) f. 50% glycerol (giycerjn) 9.20% ethanol result © mixed okra solution and cosmetic polymer moisturizing raw material, its compatibility is 疋'There is no turbidity, so it can be confirmed that the okra mucus is suitable for use as a moisturizer in cosmetic raw materials. Example 4: Okra ingredients for skin safety test Allergy test Will okra extract sticky component sails with different solvent groups and 1〇〇 £〇 different agent group, coated in microlipids, and made into an allergy patch, and extracted from the okra extracted in C, 1〇GC, respectively, without being coated on 26 200930411 - microlipid The allergic patches made in the granules were used as the control group and were attached to the upper arms of 20 volunteers. They were removed after 24 hours. The subjects were observed to have skin irritation or allergies, according to skin reactions. The scoring method of the evaluation, and the results are recorded, and the evaluation of the skin reaction is used as a score sheet. The score. (Draize, F., Woodard, G., Calvery, H. 0) 1948
Appraisel of the safety of chemicals in food, drugs and cosmetics. Pharmacologic. 93:377-392.) 結果 根據皮膚反應評價的評分法，受測者完全沒有產生皮 膚刺激感或過敏現象情形’因此能確認黃秋葵黏質成份及 有包覆微脂粒之黃秋葵黏質成份皆是安全無刺激性的。 實施例五：包覆黃秋葵萃取物之微脂粒製備與測試 1 微脂粒溶液之製備 微脂粒配製： Q 取微脂粒加入0.25g膽固醇及i〇g無水乙醇均勻 混合使其溶解’設定加熱萃取的條件為80〇C和1〇〇。〇，依 萃取溶劑比例的不同及萃取設定加熱温度的不同共分為1 〇 個實驗組’在80。〇萃取條件下依溶劑及比例的不同，又分 為5種不同的實驗組別；在1 〇〇它萃取條件下依溶劑及比 例的不同’也分為5種不同的實驗組別。 配製方法： 取在80°C下以5個不同溶劑組所萃取的黃秋葵萃取物 黏質成份，配製成5%溶液備用。 27 200930411 ， 取在1 00°C下以5個不同溶劑組所萃取的黃秋葵萃取 物黏質成份，配製成5%溶液備用。 將上述共1 0個實驗組所調配成之5%黃秋葵黏質萃取 液，每組各取8 ml萃取液，以乙醇注射法注入2 ml前述 配製完成的微脂粒溶液，並分別以超音波細胞粉碎機在 9W(輸出功率）下震盪15分鐘，完成微脂粒的製備。下表 四為萃取液以微脂粒包覆之比例： 表四：80°C及1 〇〇°C之配方包覆微脂粒之比例： ❹ _ 微指粒包覆比例（重量比） 配方 - 80% 比 20% A-P-01 A-01 80% 微脂粒溶液20% A-P-02 A-02 80% 微脂粒溶液20% A-P-03 A-03 80% 微脂粒溶液20% A-P-04 A-04 80% 微脂粒溶液20% A-P-05 A-05 80% 微脂粒溶液20% B-P-01 B-01 80% 微脂粒溶液20% B-P-02 B-02 80% 微脂粒溶液20% B-P-03 B-03 80% 微脂粒溶液20% B-P-04 B-04 80% 微脂粒溶液20% B-P-05 B-05 80% 微脂粒溶液20% 2.微脂粒電位及粒徑的量測 以粒徑分析儀：高濃度奈米粒徑分析儀，MALVERN， Zetasizer Nano ZS。將製備好的微脂粒溶液，取1 ml放 28 200930411 - 置粒徑分析管内進行粒徑量測，求取粒徑及表面電位平均 值；之後將1 〇〇°C、80°c溶劑萃取組之黃秋葵黏質成份包 覆在微脂粒中，以超音波震盪15分鐘後量測其粒徑及電 位。 結果 請參閱下表四，其為本發明之微脂粒包覆黃秋葵萃取 物之平均粒徑及平均表面電位值，另請一併參考第四圖， 其說明本發明之微脂粒平均粒徑。由乙醇當溶劑的A-02、 B-02，其微脂粒的平均粒徑較小，溶液也呈透明狀態，電 位差也比較大，觀察14天後並沒有沉澱產生；其餘溶劑 組的平均粒徑反而比較大，溶液也顯現出一點霧白不透明 狀，電位差也比較小，觀察14天後有一些沉澱產生。這 個現象符合粒子愈小電位差愈大且越穩定的理論。 表四：微脂粒包覆黃秋葵萃取物之平均粒徑及平均表面電位值 (N = 4，(Mean土S.D·))_ 成分 平均表面粒徑 (nm) 平均表面電位 (mV) 成分 平均表面粒徑 (nm) 平均表面電位 (mV) B-P-01 177.00±1.00 -2_16±0·12 Α-Ρ-01 172.00±4.00 -1.53±0.16 B-P-02 122.67±0.58 -5.29±0.78 Α-Ρ-02 112.33±1.53 -2.47±0.29 B-P-03 200.00±2.65 -1.88±0.09 Α-Ρ-03 129.33±1.15 -2.55±0.29 B-P-04 177.00±2.00 -3.42±0.22 Α-Ρ-04 162.67±4.62 -1.70±0.43 B-P-05 194.33±2.52 -1.91+0.09 Α-Ρ-05 178.67±2.08 -2.34±0.48 3.微脂粒增加黏稠度的量測 29 200930411 微脂粒包覆形成後因其粒子與電位關係，常會因為靜 電原因而容易形成凝聚現象，變成為較大顆粒，並產生分 離或"L殿現象，所以為了防止因靜電而產生的凝聚現象， 因此利用增加其|❻度來使懸浮微脂粒產生共價鍵以形成 較穩定的狀態。 配製方法Pharmacologic. 93:377-392.) Results According to the scoring method of skin reaction evaluation, the subject did not have any skin irritation or allergic condition. Therefore, it was confirmed that the okra was sticky. The ingredients and the ovy-coated musks are safe and non-irritating. Example 5: Preparation and testing of microlipids coated with okra extract 1 Preparation of microlipid solution Preparation of microlipid: Q Take 0.25 g of cholesterol and i〇g absolute ethanol and mix them evenly to dissolve. The conditions for the heating extraction were 80 ° C and 1 Torr. 〇, depending on the ratio of the extraction solvent and the heating temperature of the extraction setting, the total is divided into 1 实验 experimental group ' at 80. Under the extraction conditions, the solvent and the ratio were divided into five different experimental groups; under the conditions of 1 〇〇, the solvent and the ratio were different, and they were also divided into five different experimental groups. Preparation method: The viscosity component of the okra extract extracted from 5 different solvent groups at 80 ° C was prepared and prepared into a 5% solution for use. 27 200930411 , Take the viscous component of the okra extract extracted from 5 different solvent groups at 100 °C, and prepare a 5% solution for use. The above-mentioned 10 experimental groups were prepared into 5% okra muscow extract, 8 ml of each extract was taken, and 2 ml of the prepared microlipid solution was injected by ethanol injection, and ultrasonic waves were respectively prepared. The cell pulverizer was shaken at 9 W (output power) for 15 minutes to complete the preparation of the vesicles. Table 4 below shows the ratio of the extract to the micro-lipid: Table 4: Proportion of the coated lipid particles at 80 ° C and 1 ° C: ❹ _ Micro-finger coating ratio (weight ratio) Formulation - 80% to 20% AP-01 A-01 80% liposome solution 20% AP-02 A-02 80% liposome solution 20% AP-03 A-03 80% liposome solution 20% AP- 04 A-04 80% liposome solution 20% AP-05 A-05 80% liposome solution 20% BP-01 B-01 80% liposome solution 20% BP-02 B-02 80% lipoprotein Granule solution 20% BP-03 B-03 80% microlipid solution 20% BP-04 B-04 80% microlipid solution 20% BP-05 B-05 80% microlipid solution 20% 2. Minilipid Particle potential and particle size were measured by particle size analyzer: high concentration nano particle size analyzer, MALVERN, Zetasizer Nano ZS. Prepare the prepared liposome solution, take 1 ml and place it in 200930411 - Set the particle size measurement tube to measure the particle size, and obtain the average particle size and surface potential; then extract the solvent at 1 °C and 80 °C. The musk mixture of the okra was coated in the vesicles, and the particle size and potential were measured after 15 minutes of ultrasonic vibration. For the results, please refer to the following Table 4, which is the average particle size and average surface potential value of the oligosaccharide-coated okra extract of the present invention, and please refer to the fourth figure, which illustrates the average particle size of the vesicles of the present invention. . From ethanol to solvent A-02, B-02, the average particle size of the microlipids is small, the solution is also transparent, and the potential difference is relatively large. After 14 days of observation, no precipitation occurs; the average particle size of the remaining solvent group The diameter is rather large, and the solution also shows a little white opacity, and the potential difference is relatively small. Some precipitates are produced after 14 days of observation. This phenomenon is consistent with the theory that the smaller the potential difference is, the more stable and stable the particle is. Table 4: Average particle size and average surface potential value of oligosaccharide-coated okra extract (N = 4, (Mean soil SD·)) _ component average surface particle size (nm) average surface potential (mV) component average surface Particle size (nm) Average surface potential (mV) BP-01 177.00±1.00 -2_16±0·12 Α-Ρ-01 172.00±4.00 -1.53±0.16 BP-02 122.67±0.58 -5.29±0.78 Α-Ρ-02 112.33±1.53 -2.47±0.29 BP-03 200.00±2.65 -1.88±0.09 Α-Ρ-03 129.33±1.15 -2.55±0.29 BP-04 177.00±2.00 -3.42±0.22 Α-Ρ-04 162.67±4.62 -1.70± 0.43 BP-05 194.33±2.52 -1.91+0.09 Α-Ρ-05 178.67±2.08 -2.34±0.48 3. Measurement of viscosity increase of vesicles 29 200930411 After the formation of viscous particles, due to the relationship between particles and potential, Often due to static electricity, it is easy to form agglomeration, become larger particles, and produce separation or "L temple phenomenon, so in order to prevent the condensation caused by static electricity, so increase the | A covalent bond is created to form a more stable state. Preparation method
矛J用磷:酸類緩衝溶液將微脂粒調整pH值在6·5左右， 加入—仙膠’並使其均勻分散’丨中有微脂粒包覆之黃秋 葵黏質成M8G C及1 QQt的不同溶劑組）增加其黏稠度後， 在不同環境中觀察28 A ’結果顯示並沒有沉殿、混濁情 形產生，證明增加黏稠度可以穩定微脂粒。 4.微脂粒安定性的量測 。π鐵恆溫試驗：將有包覆微脂粒黃秋葵黏質成份之 80C及1〇(rC不同溶劑組放置於5〇。〇惶溫箱中，每日取出 够其變化，觀察時間28 *。結果顯示並沒有沉殿、混 濁、味道、顏色、粘度變化情形產生，證明微脂粒增稠後， 在高溫的環境中是穩定的。 不同溫度對微脂粒粒徑安定性之影響：包覆黃秋葵萃 取成份之微脂粒溶液，分別置於-2(TC、4°C、37t和室溫 四種條件下，在28天的儲藏期間内，分別於（7、14、21 28天）4個時間點取樣，觀察微脂粒粒徑之變化情形，只 有在零下_2G°C條件下有產生結冰外其餘條件下除己醇萃取 物質顏色變成較淡外，其餘均無明顯變化。因為溶劑在·2(Γ(： 下已達到結冰温度，但回到常温後即恢復正常並沒有產生 30 200930411 , 變化。不同磷酸類緩衝溶液pH值對微脂粒粒徑安定性之 影響：文獻顯示微脂粒儲存的最佳pH值為6.5，因此將包 覆黄秋葵萃取成份之微脂粒分別儲存於磷酸類緩衝溶液ρΗ 值為5.9、6_5、6.8、7.4、8_0之碟酸類緩衝溶液中，置 於室溫下’經7、14、21 ' 28天後取出，觀察微脂粒粒徑 之變化’結果並沒有產生變化，故能確認在pH值在5_9〜 8.0範圍内是穩定的。 ❹ 實施例六：黃秋葵萃取成份及包覆黃秋葵成份微脂粒對 皮膚的有效性測試 1.皮膚保濕度之測試： 黃秋葵萃取成份與微脂粒包覆黃秋葵成份的兩組樣品 均勻塗抹於人體手臂内側皮膚上，在相對濕度6〇_65%、 溫度25°C左右以皮膚角質保濕能力分析儀（CK c〇l〇gne GERMANY，CORNEOMETER 825pc)的探針測量任 ❹取六點’偵測經不同時間後’手臂皮膚電容量的增加率。 測試方法： 取loot萃取條件下的5個不同溶劑組所萃取黃秋婆 的黏質成&，& 8G C萃取條件下的5個不同溶劑組所萃取 黃秋葵的黏質成份，分別、給1〇個自願測試人員做測試。 另外將上述兩條件所萃取黃秋葵黏質成份調配成州，包 覆在微脂粒中，並以同姆tΛ π, μ j J樣方去分別給1 〇個自願測試人員 做測試。 幻U濕度測:將帆及1GG°C溶劑組所萃取 31 200930411 * . 之黃秋葵黏質成份及有包覆微脂粒之黃秋葵黏質成份兩組 測試做比較，其中兩者最初5分鐘量測的結果，保濕度比 原先未塗抹樣品前的時候還高出許多，但經過30分鐘後 量測的結果，下降速度非常明顯，反而比原先未測試前的 保濕度還差，每間隔30分鐘再量測，共四次2小時，結 果並沒有明顯地比原先未塗抹前良好，有包覆微脂粒之黃 秋葵成份因含有20%乙醇，所以有可能因乙醇揮發而連帶Spear J with phosphorus: acid buffer solution to adjust the pH of the liposome to about 6.5, add - Xianjiao 'and make it evenly dispersed '丨 有 有 微 有 有 黄 黄 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成 成After increasing the viscosity of the different solvent groups, the observation of 28 A ' in different environments showed no stagnation or turbidity, indicating that increasing the viscosity can stabilize the vesicles. 4. Measurement of the stability of the vesicles. π iron constant temperature test: 80C and 1〇 (rC different solvent groups coated with lipogranular okra) will be placed in 5 〇. In a thermostat, the change is taken daily, and the observation time is 28 *. It shows that there is no change in the hall, turbidity, taste, color and viscosity, which proves that the micro-lipids are thickened and stable in a high temperature environment. The effect of different temperatures on the stability of the micro-lipid size: coated okra The liposome solution of the extracted components was placed at -2 (TC, 4 ° C, 37 t and room temperature, respectively, during the 28-day storage period, respectively (7, 14, 21 28 days) 4 times Sampling and observing the change of the particle size of the micro-lipids, except that under the conditions of freezing at 2:00 G °C, the color of the hexanol extract was lighter, and the others did not change significantly. · 2 (Γ(: The freezing temperature has been reached, but it returns to normal after returning to normal temperature and does not produce 30 200930411, change. The effect of different phosphoric acid buffer solution pH on the stability of microlipid particle size: the literature shows micro The optimal pH for storage of fat particles is 6.5, so The microlipids of the extracts of the okra are stored in a buffer solution of a phosphate buffer solution with a pH value of 5.9, 6_5, 6.8, 7.4, and 8_0, and placed at room temperature for '7, 14, 21' after 28 days. The result of taking out and observing the change in the particle size of the microlipids did not change, so it was confirmed that the pH was stable in the range of 5_9 to 8.0. 实施 Example 6: Extract of okra and the composition of the tallow particles coated with okra Skin Efficacy Test 1. Skin Moisture Test: Two sets of samples of okra extract and oligosaccharide-coated okra are evenly applied to the inner skin of the human arm at a relative humidity of 6〇_65% and a temperature of 25°C. The probe of the skin keratin moisturizing capacity analyzer (CK c〇l〇gne GERMANY, CORNEOMETER 825pc) can be used to measure the increase rate of the skin energy of the arm after six points of 'detection after different time. Test method: take loot The cohesive components of the yellow okra extracted from the five different solvent groups of the extracts of Huangqiu under the extraction conditions were extracted and tested, respectively. The test personnel did the test. In addition, the musk extracts extracted from the above two conditions were formulated into states, coated in the vesicles, and each of them was given to 1 自愿 voluntary tester by the same tw, π, μ j J Test. Fantasy U Humidity Test: Compare the two components of the stick component of the sail and the 1GG °C solvent group 31 200930411 *. The okra muscoid component and the ooliate coated okra stickiness component, the first 5 minutes of the test. As a result of the measurement, the moisturizing degree was much higher than that before the original sample was not applied. However, after 30 minutes, the measurement results showed that the falling speed was very obvious, but it was worse than the original moisturizing degree before the test. Minutes were measured again for 4 times and 2 hours. The results were not significantly better than before. The okra coated with vesicles contained 20% ethanol, so it may be associated with ethanol evaporation.
將皮膚水份帶走，本發明之黃秋葵萃取黏質成份有不含乙 A 醇部份，經塗抹後其電容量雖然比未塗抹前保濕度佳，但 比5分鐘時的量測值下降很多，在含有乙醇成份之樣品經 塗抹後其電容量比未塗抹前保濕度很明顯下降，其中由表 五及表六結果推斷，是因為塗抹之成份受體温影響而將皮 膚表面水份連同塗抹之成份一同揮發出來的關係。故能推 斷此種測試方法容易受到温度、濕度、及萃取物質之溶劑 等内外在因素的影響。 表五：皮膚保濕測量分析表（有包覆微脂粒） 〇 - 測試前 5分鐘 30分鐘 60分鐘 90分鐘120分鐘 配方 B-P-01 68.50±3.27 108.60±7.20 61.40±2.30 61.60l1.52 59.80±6.06 58.80±3.96 B-P-02 73.00±3.38 88.80±4.44 56.60±4.39 58.80±3.42 57.40±7.70 56.80±3.56 B-P-03 62.00±6.60 110.00±5.20 69.80±5.07 58.80±1.79 54.20±6.22 64.40±2.61 B-P-04 52.20+5.17 108.80±3.11 66.40±5.41 61.00±4.12 62.20±4.02 61.00±7.68 B-P-05 60.00±6.20 113.20±3.63 53.00±2.92 53.40±2.88 59.80±6.46 61.40±6.19 A-P-01 ~~70.00±4.30 119.20±0.84 72.00±2.55 81.60±4.28 75.00±2.55 74.40±1.14 32 200930411 A-P-02 76.80±3.63 104.80±8.84 77.00±5.59 83.80±2.59 75.40±3.91 74.60±4.04 A-P-03 51.80±3.70 111.20±3.77 77.40±5.59 55.80±4.15 65.80±2.17 44.60±2.79 A-P-04 73.20±6.30 102.00±2.74 64.40±3.13 65.80±2.17 63.00±2.35 63.40±1.82 A-P-05 44.20±3.90 116.20±1.30 43.20±2.77 44.60±2.79 39.40±3.05 43.40±3.85 單位：a_u. (N = 10，Mean±S.D.) 表六：皮膚保濕測量分析表（無包覆微脂粒） 配方 測試前 5分鐘 30分鐘 60分鐘 90分鐘 120分鐘 B-01 54.00±6.35 107.40±3.21 43.00±1.41 44.20±2.49 41.60±2.61 45.20±2.59 B-02 60.40±51.8 ' 79.20±7.46 54.60±1.95 56.20±3.35 56.20±4.32 61.2013.35 B-03 57.40±2.07 109.60+3.21 78.60±6.15 62.4013.85 59.60±4.77 55.80±2.59 B-04 88.00±3.94 104.40±5.46 79.40±3.91 85.20±3.03 80.00±1.22 77.40±2.07 B-05 94.60±4.34 116.60±1.67 73.40±1.95 80.20±4.15 79.40±3.58 77.20±2.17 A-01 60.60±3.51 97_60±13_28 54.00±2.65 49.60±2.30 50.2011.30 54.60±1.52 A-02 57.60±5.59 68.40±7.44 50.8013.11 58.00±2.12 53.20±2.49 55.60±1.52 A-03 52.20±4.55 82.20±11.45 57.20±2.49 60.80±1.64 59.40±2.51 59.80±3.35 A-04 63.40±2.41 95.80±6.61 62.2011.64 64.20±3.42 60.60±2.88 59.60±3.05 A-05 47.80±2.17 116.60±1.67 56.6012.61 56.0013.39 67.40±2.51 59.80±3.35 單位：a.u. (N = 1 0,Mean±S.D.) 2.皮膚彈力度之測試： 黃秋葵萃取成份與微脂粒包覆黃秋葵成份的兩組樣 品、共20個檢測組均勻塗抹於人體手臂皮膚上，在相對 濕度60-65%、溫度25°C左右以皮膚彈性測定儀Cutometer @ MPA580的探針測量認取六點，經不同日期偵測後，看 手臂皮膚彈性度的增加率，其皮膚彈性指標如下表七所示： 33 200930411 . 表七：皮膚彈性指標 RO-曲線最高點 R1-曲線最底點 R2-彈性明顯度，數值越接近1，越有性 R3-最後曲線最高度 R4-最後量測點 R5-沒有彈性點 R6-數值越小彈性越大 R7·部份彈性點 R8-值越大皮膚彈躍情形越好 R9-游標鍵 測試方法： 取100C萃取條件下5個不同溶劑組所萃取的黃秋葵 黏質成份及在8(Tc萃取條件下5個不同溶劑組所萃取的黃 秋葵黏質成份，分別給，〇個自願測試人員做測試。另外 將上述兩條件所萃取黃秋蔡黏質成份調配成5%，包覆在 微知粒中，並以同樣方法分別給1 0個自願測試人員做測 ❹ 試。 本發明係利用純水、乙醇、1,3 丁二醇（Butylene Glycol) 一種溶劑’使用單獨和不同比例的混合來萃取黄秋葵的黏 物2成份’經由紫外光/可見光光度計量測其黏物質成份， 超θ波細胞粉碎機製備微脂粒，奈米粒徑分析儀測量粒徑 及電位，由虎1& I屑角質保濕能力分析儀測量皮膚之保濕度，皮 膚彈力'則試儀測量皮膚之彈性度，其中取80°C、1 00°C黃 秋蔡萃取_ & B z 抑及有包覆微脂粒之黃秋葵萃取物、經塗抹皮膚 後做彈力測兮 . j咸’每天早晚各塗抹一次，每星期量測一次共 34 200930411 , 量測四次28天，由表八至表十一量測結果顯示：R2值及 R8值都有緩緩上升超過原先皮膚未塗時之量測值，其中R8 數值越高，表示皮膚彈躍恢復情況越佳，R2值為彈性明顯 度，數值越接近1，愈有彈性，另N = 10(Mean±S_D_)， 因此顯示本發明之黃秋葵萃取物及有包覆微脂粒之黃秋葵 萃取物確實都能增加皮膚的彈性效果，整體而言有包覆微 脂粒成份之效果比黃秋葵萃取黏物質效果顯著。 ® 表八：黃秋葵萃取物之皮膚質彈力測試R8值分析表（有包覆 微脂粒） 配方 測試前 第1週 第2週 第3週 第4週 B-P-01 0.29±0.05 0.31±0_07 .29±0.02 0.25±0.03 0.28±0.02 B-P-02 0.25+0.02 0.23±0.02 0.28±0_02 0.23±0.01 0.29±0.02 B-P-03 0.26±0.04 0.25±0.04 0.3310.04 0.29±0.01 0.31 ±0.05 B-P-04 0_22±0.04 0.24±0.03 0.29±0.04 0.19±0.01 0.28±0.04 B-P-05 0.12±0.03 0.31 ±0.03 0.24±0.01 0.2110.04 0.26±0.03 A-P-01 0.25±0.05 0.25±0.01 0.33±0.03 0.28±0.03 0.33+0.05 A-P-02 0.22±0.05 0.22±0.02 0.19±0.04 0.21 ±0.02 0.21±0.01 A-P-03 0.22±0.05 0.22±0.02 0.19±0.04 0.19±0.01 0.31±0.03 A-P-04 0.16±0.03 0.22±0.04 0_21±0.01 0.24±0.03 0.27±0.03 A-P-05 0.15±0.02 0.20±0.02 0.20±0.041 0.14±0.02 0.16±0.02The skin moisture is taken away, and the extract component of the okra of the present invention does not contain the ethyl alcohol component. After the application, the electric capacity is better than that before the unapplied, but the measurement value is much lower than that at the 5 minute. After the smear of the sample containing the ethanol component, the urinary capacity of the sample after the smear is significantly lower than that of the unapplied sample. It is inferred from the results of Tables 5 and 6 that the surface moisture of the skin is applied together with the temperature of the smear component. The relationship between the ingredients and the volatilization. Therefore, it can be inferred that such a test method is susceptible to internal and external factors such as temperature, humidity, and solvent of the extracted substance. Table 5: Skin Moisture Measurement Analysis Form (with coated lipid particles) 〇 - 5 minutes before the test 30 minutes 60 minutes 90 minutes 120 minutes Formulation BP-01 68.50±3.27 108.60±7.20 61.40±2.30 61.60l1.52 59.80±6.06 58.80±3.96 BP-02 73.00±3.38 88.80±4.44 56.60±4.39 58.80±3.42 57.40±7.70 56.80±3.56 BP-03 62.00±6.60 110.00±5.20 69.80±5.07 58.80±1.79 54.20±6.22 64.40±2.61 BP-04 52.20+ 5.17 108.80±3.11 66.40±5.41 61.00±4.12 62.20±4.02 61.00±7.68 BP-05 60.00±6.20 113.20±3.63 53.00±2.92 53.40±2.88 59.80±6.46 61.40±6.19 AP-01 ~~70.00±4.30 119.20±0.84 72.00± 2.55 81.60±4.28 75.00±2.55 74.40±1.14 32 200930411 AP-02 76.80±3.63 104.80±8.84 77.00±5.59 83.80±2.59 75.40±3.91 74.60±4.04 AP-03 51.80±3.70 111.20±3.77 77.40±5.59 55.80±4.15 65.80± 2.17 44.60±2.79 AP-04 73.20±6.30 102.00±2.74 64.40±3.13 65.80±2.17 63.00±2.35 63.40±1.82 AP-05 44.20±3.90 116.20±1.30 43.20±2.77 44.60±2.79 39.40±3.05 43.40±3.85 Unit: a_u. (N = 10, Mean ± SD) Table 6: Skin moisturization test Quantitative analysis table (without coated vesicles) 5 minutes before formula test 30 minutes 60 minutes 90 minutes 120 minutes B-01 54.00±6.35 107.40±3.21 43.00±1.41 44.20±2.49 41.60±2.61 45.20±2.59 B-02 60.40± 51.8 ' 79.20±7.46 54.60±1.95 56.20±3.35 56.20±4.32 61.2013.35 B-03 57.40±2.07 109.60+3.21 78.60±6.15 62.4013.85 59.60±4.77 55.80±2.59 B-04 88.00±3.94 104.40±5.46 79.40±3.91 85.20±3.03 80.00±1.22 77.40±2.07 B-05 94.60±4.34 116.60±1.67 73.40±1.95 80.20±4.15 79.40±3.58 77.20±2.17 A-01 60.60±3.51 97_60±13_28 54.00±2.65 49.60±2.30 50.2011.30 54.60± 1.52 A-02 57.60±5.59 68.40±7.44 50.8013.11 58.00±2.12 53.20±2.49 55.60±1.52 A-03 52.20±4.55 82.20±11.45 57.20±2.49 60.80±1.64 59.40±2.51 59.80±3.35 A-04 63.40±2.41 95.80 ±6.61 62.2011.64 64.20±3.42 60.60±2.88 59.60±3.05 A-05 47.80±2.17 116.60±1.67 56.6012.61 56.0013.39 67.40±2.51 59.80±3.35 Unit: au (N = 1 0, Mean±SD) 2. Test of skin elasticity: Okra extract and emollient coated okra ingredients Two groups of samples, a total of 20 test groups were evenly applied to the skin of the human arm. The relative humidity of 60-65% and the temperature of 25 °C were measured by the probe of the skin elasticity tester Cutometer @ MPA580. After detection, look at the increase rate of arm skin elasticity, the skin elasticity index is shown in Table 7 below: 33 200930411 . Table 7: Skin elasticity index RO-curve highest point R1 - curve bottom point R2-elasticity, value The closer to 1, the more sexually R3-the last curve is the highest height R4-the last measurement point R5-there is no elastic point R6-the smaller the value, the greater the elasticity R7·the more the elastic point R8-the larger the value of the skin, the better the situation - vernier key test method: Take the viscosity component of okra extracted by 5 different solvent groups under 100C extraction conditions and the viscosity component of okra extracted by 5 different solvent groups under Tc extraction conditions, respectively, give a voluntary The tester does the test. In addition, the above-mentioned two conditions were extracted into the 5% of the Qiqiu, and coated in the micro-particles, and 10 volunteer testers were tested in the same way. The invention utilizes pure water, ethanol, butyl butyl diol (Butylene Glycol), a solvent 'extracting the viscous component 2 of the okra using a mixture of different and different ratios' to measure the viscous substance by ultraviolet/visible luminosity measurement. Ingredients, Super-theta wave cell pulverizer to prepare micro-lipids, nanometer particle size analyzer to measure particle size and potential, skin humidity measured by Tiger 1 & I chip keratin moisturizing capacity analyzer, skin elasticity 'measured by skin tester The degree of elasticity, which is taken at 80 ° C, 100 ° C, Huang Qiu Cai extract _ & B z and the okra extract coated with vesicles, after the application of the skin to do the elastic test. j salt 'every morning and evening Each application is applied once a week, totaling 34 200930411, measuring four times and 28 days. The results from Table 8 to Table 11 show that both R2 and R8 values have risen slowly over the original uncoated skin. The measured value, wherein the higher the R8 value, the better the skin rebound recovery, the R2 value is the elastic visibility, the closer the value is to 1, the more elastic, and the other N = 10 (Mean ± S_D_), thus showing the okra of the present invention. Extract and yellow autumn with coated lipid particles Extracts can actually increase the elasticity of the skin effect, on the whole it has the effect of coated vesicles ingredient okra extract significantly than sticky substance effect. ® Table 8: Skin Essence Test for Okra Extract R8 Value Analysis Table (with coated liposome) 1 week before the test. Week 2 Week 3 Week 4 BP-01 0.29±0.05 0.31±0_07 .29 ±0.02 0.25±0.03 0.28±0.02 BP-02 0.25+0.02 0.23±0.02 0.28±0_02 0.23±0.01 0.29±0.02 BP-03 0.26±0.04 0.25±0.04 0.3310.04 0.29±0.01 0.31 ±0.05 BP-04 0_22±0.04 0.24±0.03 0.29±0.04 0.19±0.01 0.28±0.04 BP-05 0.12±0.03 0.31 ±0.03 0.24±0.01 0.2110.04 0.26±0.03 AP-01 0.25±0.05 0.25±0.01 0.33±0.03 0.28±0.03 0.33+0.05 AP- 02 0.22±0.05 0.22±0.02 0.19±0.04 0.21 ±0.02 0.21±0.01 AP-03 0.22±0.05 0.22±0.02 0.19±0.04 0.19±0.01 0.31±0.03 AP-04 0.16±0.03 0.22±0.04 0_21±0.01 0.24±0.03 0.27 ±0.03 AP-05 0.15±0.02 0.20±0.02 0.20±0.041 0.14±0.02 0.16±0.02
表九：黃秋葵萃取物之皮膚質彈力測試R8值分析表（無包覆 35 200930411 微脂粒） 配方 測試前 第1週 第2週 第3週 第4週 B-01 0.13±0.02 0.17±0.02 0.15±0_01 0.15±0.02 0.24±0.02 B-02 0.30±0.01 0.22±0.03 0.28±0.05 0.27±0.02 0.33±0.10 B-03 0.26±0.05 0_36±0.04 0.2710.01 0.27±0.09 0.37±0.05 B-04 0.27±0.02 0.32±0.03 0.26±0.02 0.26±0.01 0.27±0.01 B-05 0.26±0.01 0.28±0.02 0.29±0.03 0.22±0.02 0.30±0.03 A-01 0.24±0.01 0.21 ±0.04 0.38±0.02 0.30±0.06 0.35±0.02 A-02 0.29±0.07 0.34±0.04 0.33±0.04 0.30±0.07 0.30±0.03 A-03 0.22±0.05 0.29±0.02 0.26±0.02 0.28±0.02 0.32±0.02 A-04 0.23±0.03 0.31±0.03 0.30±0.02 0.30±0.02 0.32±0.02 A-05 0.28±0.01 0.30±0.02 0.29±0.02 0.24±0.03 0.26±0.01 表十：黃秋葵萃取物之皮膚質彈力測試 微脂粒） R2值分析表 (有包覆 配方 測試前 第1週 第2週 第3週 第4週 B-P-01 0.92±0.02 0.94±0.02 0.91 ±0.02 0.91±0.02 0.92±0.02 B-P-02 0.90+0.03 0.82±0.05 0.92±0.04 0.91+0.03 0.92±0.04 B-P-03 0.92±0.01 0.92±0.02 0.93±0.00 0.92+0.01 0.95±0.01 B-P-04 0.87±0.04 0.89±0.01 0.92±0.02 0.8U0.14 0.93±0.01 B-P-05 0.80±0.03 0.93±0.01 0.92±0.02 0.90±0.02 0.93±0.02 A-P-01 0.90±0.03 0.90±0.02 0.93±0.01 0.93±0.01 0.94±0.01 A-P-02 0.87±0.04 0.83±0.07 0.87±0.04 0.90±0.02 0.92±0.02 A-P-03 0.82±0.03 0.83±0.06 0.89±0.02 0.88±0.02 0.94±0.01 36 200930411 A-P-04 0.81 ±0.05 0.86±0.01 0.89±0.01 0.90±0.01 0.92+0.02 A-P-05 0.84±0.01 0.84±0.09 0.90±0.01 0.88±0.02 0.90+0.02 表十一：黃秋葵萃取物之皮膚質彈力測試R2值分析表（無包Table 9: R8 value analysis table for skin elasticity test of okra extract (no coating 35 200930411 microlipid) 1 week before formula test 2 weeks 3 weeks 4th week B-01 0.13±0.02 0.17±0.02 0.15 ±0_01 0.15±0.02 0.24±0.02 B-02 0.30±0.01 0.22±0.03 0.28±0.05 0.27±0.02 0.33±0.10 B-03 0.26±0.05 0_36±0.04 0.2710.01 0.27±0.09 0.37±0.05 B-04 0.27±0.02 0.32±0.03 0.26±0.02 0.26±0.01 0.27±0.01 B-05 0.26±0.01 0.28±0.02 0.29±0.03 0.22±0.02 0.30±0.03 A-01 0.24±0.01 0.21 ±0.04 0.38±0.02 0.30±0.06 0.35±0.02 A- 02 0.29±0.07 0.34±0.04 0.33±0.04 0.30±0.07 0.30±0.03 A-03 0.22±0.05 0.29±0.02 0.26±0.02 0.28±0.02 0.32±0.02 A-04 0.23±0.03 0.31±0.03 0.30±0.02 0.30±0.02 0.32 ±0.02 A-05 0.28±0.01 0.30±0.02 0.29±0.02 0.24±0.03 0.26±0.01 Table 10: Skin Essence Test of Okra Extracts Lipids) R2 Analysis Form (1st week before the coated formula test) 2 weeks, 3rd week, 4th week, BP-01 0.92±0.02 0.94±0.02 0.91 ±0.02 0.91±0.02 0.92±0.02 BP-02 0.90+0.03 0.82±0.05 0.9 2±0.04 0.91+0.03 0.92±0.04 BP-03 0.92±0.01 0.92±0.02 0.93±0.00 0.92+0.01 0.95±0.01 BP-04 0.87±0.04 0.89±0.01 0.92±0.02 0.8U0.14 0.93±0.01 BP-05 0.80 ±0.03 0.93±0.01 0.92±0.02 0.90±0.02 0.93±0.02 AP-01 0.90±0.03 0.90±0.02 0.93±0.01 0.93±0.01 0.94±0.01 AP-02 0.87±0.04 0.83±0.07 0.87±0.04 0.90±0.02 0.92±0.02 AP-03 0.82±0.03 0.83±0.06 0.89±0.02 0.88±0.02 0.94±0.01 36 200930411 AP-04 0.81 ±0.05 0.86±0.01 0.89±0.01 0.90±0.01 0.92+0.02 AP-05 0.84±0.01 0.84±0.09 0.90±0.01 0.88±0.02 0.90+0.02 Table 11: R2 value analysis table for skin elasticity test of okra extract (no package)
覆微脂粒） 配方 測試前 第1週 第2週 第3週 第4週 B-01 0.92±0.02 0.94±0.02 0.91 ±0.02 0.91 ±0.02 0.92±0.02 B-02 0.90±0.03 0.85±0.05 0.92±0.04 0.91±0.03 0.92±0.04 B-03 0.92±0.01 0.92±0.02 0.92±0.00 0.92±0.01 0.950±0.01 B-04 0.87±0.04 0.89±0.01 0.92±0.02 0.81±0.14 0.93±0.01 B-05 0.80±0.03 0.93±0.01 0.92±0.02 0.90±0.02 0.93±0.02 A-01 0.90±0.03 0.90±0.02 0.93±0.01 0.93±0.01 0.94±0.01 A-02 0.87±_0.04 0.83±0.07 0.87±0.04 0.90±0.02 0.92±0.02 A-03 0.85+0.036 0.83±0.06 0.89±0.02 0.88±0.02 0.94±0.01 A-04 0.81±0.05 0.86±0.01 0.89±0.01 0.90±0.01 0.92±0.02 A-05 0.84±.0.1 0.84±0.09 0.90±0.01 0.88+0.02 0.90±0.02 依上述實施例歸納後有下列五點結論： 1 .本發明之萃取條件與溶劑的不同及溶劑組成比例， 都會影響萃取率及萃取物質之成份，所以萃取温度、萃取 時間、萃取次數與萃取物之品種都是實驗結果最後的關鍵 因素。 2.黃秋葵的萃取物與非離子界面活性劑、陰離子界面 37 200930411 活性劑、兩性界面活性劑、高分子保濕劑、溶劑、化妝品 原料的融合。經測試後其融合情況良好、所以黃秋葵萃取 物成份是非常適合使用在化妝品原料中當保濕劑且觸感也 很好》 3 _由於微脂粒放置保存一段時間會產生混濁、凝聚、 沉澱等現象，利用增加黏稠度來懸浮微脂粒，使其形成比 較穩定的形態。經過高温、恆温、不同温度與環境 '緩衝 ❹劑PH值的影響等試驗，證實在這種形態下是穩定的，所 以利用其物性變化來達到所要目的是可行的。 4.80°C、100°C黃秋葵萃取物及包覆微脂粒之黃秋葵萃 取物，經皮膚保濕度測試結果其電容量隨著測試時間延長 而變小，塗抹後5分鐘測試其電容量數值高出測試前甚多， 但30分鐘後再測試則降到比測試前還低。每隔3〇分鐘量 測一次，共12〇分鐘，結果都沒有超過原先未塗抹前的時 候好，由結果推測可能塗抹之成份受體温影響將皮膚水份 〇 連同塗抹之成份揮發出來。有包覆微脂粒之黃秋葵成份因 含有20〇/〇乙醇，所以有可能因乙醇揮發而連帶將皮膚水份 帶走。另外不含乙酵萃取之黃秋葵萃取物黏質成份雖然比 未塗抹則保濕度較尚一些，但跟5分鐘時測試此還是下降 很多，含有乙醇成份之黃秋葵萃取物經塗抹後皮膚保濕還 是比未塗抹前效果較差。 5.80°C、100°C黃秋葵萃取物及有包覆微脂粒之黃秋葵 萃取物、經皮膚彈力測試結果證實全部成份都是有效的。 但測試結果有包覆微脂粒的R2值與R8值平均增加率比未 38 率高。根據本 比沒有包覆微 水之特性會把 並非對本發明 具有通常知識 内，利用本發 等效實施例， 於本發明技術 200930411 包覆微脂粒黏物質的R2 “每 貞的R2值與_平均增加 發月之實施例可以推斷古〜& 斷有包覆微脂粒之效果 月曰粒之黏物質效果較為 北 马顯者。符合微脂粒親 汽秋葵萃取物帶入f 、 ▼八更，木層的皮膚層裹面。 乂上所述’僅是本發明的較佳實施例， 作任何形式上的限制，4 任何所屬技術領域中 者，若在不脫離本發明所提技術特徵的範圍 明所揭示技術内容所作出局部更動或修飾的 並且未脫離本發明的技術特徵内纟，均仍屬 特徵的範圍内。 【圖式簡單說明】 第一圖係本發明之實驗流程圖。 第二圖係本發明之多醣體標準濃度檢量線圖。 第三圖係本發明之蛋白質標準濃度檢量線圖。 〇 第四圖係本發明之微脂粒平均粒徑圖。 【主要元件符號說明】 無 39Lipid coating) Formula 1 week 2 week 3rd week 4th week B-01 0.92±0.02 0.94±0.02 0.91 ±0.02 0.91 ±0.02 0.92±0.02 B-02 0.90±0.03 0.85±0.05 0.92±0.04 0.91±0.03 0.92±0.04 B-03 0.92±0.01 0.92±0.02 0.92±0.00 0.92±0.01 0.950±0.01 B-04 0.87±0.04 0.89±0.01 0.92±0.02 0.81±0.14 0.93±0.01 B-05 0.80±0.03 0.93± 0.01 0.92±0.02 0.90±0.02 0.93±0.02 A-01 0.90±0.03 0.90±0.02 0.93±0.01 0.93±0.01 0.94±0.01 A-02 0.87±_0.04 0.83±0.07 0.87±0.04 0.90±0.02 0.92±0.02 A- 03 0.85+0.036 0.83±0.06 0.89±0.02 0.88±0.02 0.94±0.01 A-04 0.81±0.05 0.86±0.01 0.89±0.01 0.90±0.01 0.92±0.02 A-05 0.84±.0.1 0.84±0.09 0.90±0.01 0.88+0.02 0.90±0.02 According to the above examples, the following five conclusions are summarized: 1. The extraction conditions of the present invention differ from the solvent and the solvent composition ratio, which affect the extraction rate and the composition of the extracted material, so the extraction temperature, extraction time, and extraction times The variety with the extract is the final key factor of the experimental results. 2. Extract of okra with nonionic surfactant and anionic interface 37 200930411 Fusion of active agent, amphoteric surfactant, polymer humectant, solvent and cosmetic raw materials. After the test, the fusion condition is good, so the okra extract component is very suitable for use as a moisturizer in the cosmetic raw materials and the touch is also good. 3 _Because the microlipid is stored for a period of time, it will cause turbidity, condensation, precipitation, etc. The viscosity is increased to suspend the vesicles to form a relatively stable morphology. After high temperature, constant temperature, different temperature and environmental impacts of buffer pH, it is confirmed that it is stable in this form, so it is feasible to use its physical properties to achieve the desired purpose. 4.80 ° C, 100 ° C okra extract and okra-coated extract of okra, the skin moisture test results show that its capacitance decreases with the test time, the capacitance value is higher after 5 minutes of application. There were a lot of tests before, but after 30 minutes, the test fell to a lower level than before the test. The measurement was performed every 3 minutes, for a total of 12 minutes, and the results did not exceed the time before the original application. It was speculated that the temperature of the applied receptors could be volatilized together with the applied ingredients. The okra component coated with vesicles contains 20 〇/〇 ethanol, so it may be taken away by the evaporation of ethanol. In addition, the moisture content of the okra extract without ethyl acetate extraction is more moisturized than that of the uncoated one, but it is still much lower than the test at 5 minutes. The okra extract containing ethanol is moisturized after application. The effect before the application is poor. 5.80 ° C, 100 ° C okra extract and okra coated with micro-lipid extract, skin elasticity test results confirmed that all ingredients are effective. However, the test results showed that the average increase rate of the R2 value and the R8 value of the coated liposome was higher than that of the non-38 rate. According to the present invention, the characteristics of the non-coated micro-water will not be used in the general knowledge of the present invention, and the R2 value of each 贞 包覆 包覆 2009 304 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 The average increase in the month of the example can be inferred that the ancient ~ & broken with the effect of coating the lipid particles of the moon granules is more effective than the North Marxian. In line with the micro-lipid pro-automotive okra extract brought into f, ▼八更, the skin layer of the wood layer is wrapped. The above description is merely a preferred embodiment of the present invention, and is subject to any form of limitation, 4 in any technical field, without departing from the techniques of the present invention. The scope of the features is not limited to the technical features of the disclosed technology, and is not within the scope of the features. The first figure is the experimental flow chart of the present invention. The second figure is a calibration chart of the standard concentration of the polysaccharide of the present invention. The third figure is a line diagram of the standard concentration of the protein of the present invention. The fourth figure is the average particle size of the vesicles of the present invention. element No. Description None 39
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| CN103315931A (en) * | 2013-05-31 | 2013-09-25 | 徐艳芳 | Okra extracting solution, okra extractives, preparation methods therefor, and applications therefor |
| CN105078848A (en) * | 2015-09-22 | 2015-11-25 | 南京德元盛生物科技有限公司 | Natural moisturizing skin care product containing okra gel and preparation method |
| CN105238595A (en) * | 2015-10-09 | 2016-01-13 | 金陵科技学院 | Okra beautifying soap and preparation method thereof |
| TWI641376B (en) * | 2015-10-22 | 2018-11-21 | 中山醫學大學 | Use of okra extract for the preparation of a composition for reducing intestinal bacteria |
| CN110613650A (en) * | 2019-10-25 | 2019-12-27 | 上海伽誉生物科技有限公司 | Essence containing okra hydrolyzed glycoprotein SILKY-WSP and preparation method thereof |
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| CN103550096B (en) * | 2013-10-31 | 2015-03-04 | 金陵科技学院 | Tender okra pod facial mask essence and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103315931A (en) * | 2013-05-31 | 2013-09-25 | 徐艳芳 | Okra extracting solution, okra extractives, preparation methods therefor, and applications therefor |
| CN103315931B (en) * | 2013-05-31 | 2014-11-26 | 徐艳芳 | Okra extracting solution, okra extractives, preparation methods therefor, and applications therefor |
| CN105078848A (en) * | 2015-09-22 | 2015-11-25 | 南京德元盛生物科技有限公司 | Natural moisturizing skin care product containing okra gel and preparation method |
| CN105238595A (en) * | 2015-10-09 | 2016-01-13 | 金陵科技学院 | Okra beautifying soap and preparation method thereof |
| CN105238595B (en) * | 2015-10-09 | 2018-08-24 | 金陵科技学院 | A kind of okra beauty soap and preparation method thereof |
| TWI641376B (en) * | 2015-10-22 | 2018-11-21 | 中山醫學大學 | Use of okra extract for the preparation of a composition for reducing intestinal bacteria |
| CN110613650A (en) * | 2019-10-25 | 2019-12-27 | 上海伽誉生物科技有限公司 | Essence containing okra hydrolyzed glycoprotein SILKY-WSP and preparation method thereof |
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