CN105769727B - A kind of Skin whitening care cosmetics and preparation method thereof of the liquid extract of boiling containing sea cucumber - Google Patents
A kind of Skin whitening care cosmetics and preparation method thereof of the liquid extract of boiling containing sea cucumber Download PDFInfo
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- CN105769727B CN105769727B CN201610136992.XA CN201610136992A CN105769727B CN 105769727 B CN105769727 B CN 105769727B CN 201610136992 A CN201610136992 A CN 201610136992A CN 105769727 B CN105769727 B CN 105769727B
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- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Zoology (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a kind of Skin whitening care cosmetics and preparation method thereof of the liquid extract of boiling containing sea cucumber.The present invention forms：In terms of 100 parts of mass fractions, wherein, sea cucumber boiling liquid extract account for 0.1~1 part, natural oil lipid account for 5~20 parts, natural thickener account for 0~5 part, higher alcohol account for 0~5 part, emulsifier account for 1~25 part, preservative account for 0.1~0.5 part, essence account for 0.05~0.5 part, functional additive account for 0~5 part, water surplus；The group of the sea cucumber boiling liquid extract becomes：30~90wt% of selenka, 5~60wt% of sea cucumber polypeptide, 5~10wt% of impurity.Sea cucumber boiling liquid extract is applied in the formula of Skin whitening care cosmetics by the present invention, starts sea cucumber boiling liquid extract in the new application of skin care field, improves Holothurian machining added value.Meanwhile using natural oil lipid and natural thickener, the risk that harmful chemicals synthetic is excessively introduced into skin care item is avoided.
Description
Technical field
The present invention relates to Skin whitening care cosmetics and preparation method thereof, more particularly to a kind of whitening of the liquid extract of boiling containing sea cucumber
Skin care item and preparation method thereof.
Background technology
Sea cucumber water cooking liquid is a large amount of waste liquids generated in Holothurian machining production process, is rich in various active substance, such as sea
Join saponin(e, sea cucumber polysaccharide etc., there is extensive physiology and pharmacological activity, it is such as antimycotic, antitumor, anti-inflammatory, antiviral, anti-
Radiation improves the effects that immunity.
Past sea cucumber water cooking liquid not only causes environmental pollution by as discharging of waste liquid, but also is a kind of wasting of resources.In recent years messenger
Come to realise potential economic value in sea cucumber water cooking liquid, its answering in the fields such as food, health products of comprehensive exploitation
With.Chinese patent 201010545951.9 discloses a kind of soy sauce and brew purifying concentrate brew using sea cucumber cooking waste liquor
Method is coordinated using being extracted in sea cucumber cooking waste liquor rich in the concentrate for having the immunomodulators such as sea cucumber polysaccharide, selenka
The raw materials such as soya bean, peanut are brewed with multi-cultur es and the brew method of science rich in sea cucumber polysaccharide and saponin(e isoreactivity ingredient
Sea cucumber sog sauce.Chinese patent 201210144010.3 disclose it is a kind of using sea cucumber water cooking liquid production sea cucumber peptide and sea cucumber wine, it is main
It is that sea cucumber water cooking liquid is mixed with trypsase, Multiprotein-zinc, edible alcohol in proportion to want technique, extracted, centrifuge, drying
Sea cucumber peptide and sea cucumber wine are obtained afterwards.Chinese patent 201510021389.2 discloses a kind of preparing nutritional agents with sea cucumber water cooking liquid
Method is raw material using sea cucumber water cooking liquid, after removing heavy metal and impurity, removes insoluble matter by concentration, adds simultaneously
Sea cucumber hydrolysate, be made particle, dry cream preparation.
The selenka contained in sea cucumber boiling liquid extract is similar to ginsenoside chemical constitution, and ginsenoside is as one
Kind anti-oxidation active substance has been widely used in the numerous areas such as food, drug, cosmetics.Chinese patent
201310536432.X discloses a kind of skin-care ginseng skin care item, including lanolin, vaseline, stearic acid, sucrose tin dilaurate
The components such as ester, whitening agent, ginsenoside, triethanolamine, water have significantly moist skin effect, especially also have whitening flesh
The effect of skin.Currently, application of the sea cucumber boiling liquid extract in the fields such as food, health products obtains extensive exploitation, but it
Application in cosmetic field rarely has research at present.
Invention content
It is an object of the invention to by the extraction and research to part nutriment in sea cucumber water cooking liquid, it is a kind of right to provide
The key technology that sea cucumber water cooking liquid is recycled is effectively facilitated the development in pluralism of Holothurian machining industry；According to sea cucumber water
The effect of boil liquid extract, develops a kind of Skin whitening care cosmetics and preparation method thereof, realizes sea cucumber boiling liquid extract high added value
Using.
In order to achieve the above objectives, the present invention provides a kind of Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber, features
It is, composition includes：In terms of 100 parts of mass fractions, wherein sea cucumber boiling liquid extract account for 0.1~1 part, natural oil lipid account for 5
~20 parts, natural thickener account for 0~5 part, higher alcohol account for 0~5 part, emulsifier account for 1~25 part, preservative account for 0.1~0.5 part,
Essence account for 0.05~0.5 part, functional additive account for 0~5 part, water surplus；
The group of the sea cucumber boiling liquid extract becomes：30~90wt% of selenka, sea cucumber polypeptide 5~60wt% are miscellaneous
5~10wt% of matter；
The natural oil lipid be camellia oil, olive oil, corn oil, rapeseed oil, sesame oil, wheat-germ oil, glycerine, can
Can fat, coconut oil, palm oil, one or more mixing in beeswax, lanolin；Preferably corn oil, rapeseed oil, sesame oil,
One or more mixing in glycerine, palm oil, beeswax；
The natural thickener is natural plant starch, gelatin, guar gum, carragheen, Arabic gum, xanthans, sea
One or more mixing of phycocolloid；It is one or more mixed preferably in natural plant starch, gelatin, guar gum, xanthans
It closes；
The higher alcohol is laruyl alcohol, hexadecanol, octadecyl alcolol, hexadecanol, lanonol, cholesterol, phytosterol, octyl
One or more mixing of dodecanol；One or more mixing preferably in hexadecanol, octadecyl alcolol；
The emulsifier is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene ten
Eight alcohol, polyoxyethylene ether, one or more mixing of Vinlub 73；Preferably stearic acid, sodium hydroxide, three second
Hydramine, siloxanes, one or more mixing of polyoxyethylene laurel ether；
The preservative is methyl p-hydroxybenzoate, ethyl-para-hydroxybenzoate, propylparaben, benzene first
One or more mixing of alcohol, dichloroxylenol, benzyl chloride phenol；Preferably methyl p-hydroxybenzoate, ethyl-para-hydroxybenzoate, right
One or more mixing of nipasol；
The functional additive is vitamin E, the one or more of urea, amino acid, hyaluronic acid, sheep placenta mix
It closes；One or more mixing preferably in vitamin E, urea, amino acid；
The essence is the essence for meeting cosmetics odor type, such as：Bouquet type composition, preferably flavoring rose essence, cream jasmin france,
Lavender oil；Milk flavoured type essence.
Under preferred embodiment, the preparation method of the sea cucumber boiling liquid extract is：
S1, sea cucumber water cooking liquid pass sequentially through microfiltration systems and ultrafiltration system after centrifugation cleans, and collect efflux；
S2, the efflux obtained with NAK-9 macroporous absorbent resin adsorption steps S1；The absorption resin is eluted with water to washing
De- liquid is colourless, discards eluent；
S3,2~4 times of column volumes of macroporous absorbent resin are eluted with 10~20% ethanol solution, discards eluent；
S4,3~6 times of column volumes of macroporous absorbent resin are eluted with 40~100% ethanol solution, collects eluent；
S5, after organic solvent is evaporated off in the step S4 eluents collected, it is freeze-dried for 24 hours~72h in -70 DEG C~-40 DEG C,
Sea cucumber boiling liquid extract is made.
The present invention also provides the preparation method of the Skin whitening care cosmetics of the above-mentioned liquid extract of boiling containing sea cucumber, specific steps
For：
S1, sea cucumber boiling liquid extract, natural oil lipid, natural thickener, higher alcohol, emulsification are weighed respectively according to formula
Agent, preservative, essence, functional additive and water, it is spare；
The formula is：In terms of 100 parts of mass fractions, wherein sea cucumber boiling liquid extract accounts for 0.1~1 part, natural oil
Class account for 5~20 parts, natural thickener account for 0~5 part, higher alcohol account for 0~5 part, emulsifier account for 1~25 part, preservative account for 0.1~
0.5 part, essence account for 0.05~0.5 part, functional additive account for 0~5 part, water surplus；
The group of the sea cucumber boiling liquid extract becomes：30~90wt% of selenka, sea cucumber polypeptide 5~60wt% are miscellaneous
5~10wt% of matter；
The natural oil lipid be camellia oil, olive oil, corn oil, rapeseed oil, sesame oil, wheat-germ oil, glycerine, can
Can fat, coconut oil, palm oil, one or more mixing in beeswax, lanolin；Preferably corn oil, rapeseed oil, sesame oil,
One or more mixing in glycerine, palm oil, beeswax；
The natural thickener is natural plant starch, gelatin, guar gum, carragheen, Arabic gum, xanthans, sea
One or more mixing of phycocolloid；It is one or more mixed preferably in natural plant starch, gelatin, guar gum, xanthans
It closes；
The higher alcohol is laruyl alcohol, hexadecanol, octadecyl alcolol, hexadecanol, lanonol, cholesterol, phytosterol, octyl
One or more mixing of dodecanol；One or more mixing preferably in hexadecanol, octadecyl alcolol；
The emulsifier is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene ten
Eight alcohol, polyoxyethylene ether, one or more mixing of Vinlub 73；Preferably stearic acid, sodium hydroxide, three second
Hydramine, siloxanes, one or more mixing of polyoxyethylene laurel ether；
The preservative is methyl p-hydroxybenzoate, ethyl-para-hydroxybenzoate, propylparaben, benzene first
One or more mixing of alcohol, dichloroxylenol, benzyl chloride phenol；Preferably methyl p-hydroxybenzoate, ethyl-para-hydroxybenzoate, right
One or more mixing of nipasol；
The functional additive is vitamin E, the one or more of urea, amino acid, hyaluronic acid, sheep placenta mix
It closes；One or more mixing preferably in vitamin E, urea, amino acid；
The essence is the essence for meeting cosmetics odor type, such as：Bouquet type composition, preferably flavoring rose essence, cream jasmin france,
Lavender oil；Milk flavoured type essence；
S2, natural oil lipid, natural thickener, higher alcohol and the emulsifier for weighing step S1 after mixing, with
The frequency ultrasonic wave of 24kHz handles 10min~15min, and 90 DEG C~100 DEG C agitating and heating 20min~30min obtain oil phase；It will
Functional additive and water handle 10min~20min after mixing, with the frequency ultrasonic wave of 24kHz, and step S1 is then added
The sea cucumber boiling liquid extract weighed, 90 DEG C~100 DEG C agitating and heating 20min~30min, obtains water phase；
S3, the obtained water phases of step S2 are added in the oil phase, it is stirring while adding；Reduce mixed solution temperature
To 45 DEG C~55 DEG C, the preservative that step S1 is weighed is added, is stirred until homogeneous dissolving；Continue to reduce solution temperature to 30 DEG C~40
DEG C, the essence that step S1 is weighed is added, is stirred until homogeneous dissolving；Solution temperature is finally reduced to room temperature, is adjusted with sodium hydroxide
Solution ph obtains mixed liquor to 7.5~8.5；
S4, the 100 DEG C of sterilization 10min~15min of mixed liquor for obtaining step S3 are to get the liquid extract of boiling containing sea cucumber
Skin whitening care cosmetics.
Under preferred embodiment, the preparation method of sea cucumber boiling liquid extract described in step S1 is：
S1, sea cucumber water cooking liquid pass sequentially through microfiltration systems and ultrafiltration system after centrifugation cleans, and collect efflux；
S2, the efflux obtained with NAK-9 macroporous absorbent resin adsorption steps S1；The absorption resin is eluted with water to washing
De- liquid is colourless, discards eluent；
S3,2~4 times of column volumes of macroporous absorbent resin are eluted with the ethanol solution of 10~20v%, discards eluent；
S4,3~6 times of column volumes of macroporous absorbent resin are eluted with the ethanol solution of 40~100v%, collects eluent；
S5, after organic solvent is evaporated off in the step S4 eluents collected, it is freeze-dried for 24 hours~72h in -70 DEG C~-40 DEG C,
Sea cucumber boiling liquid extract is made.
The advantages and positive effects of the present invention are：
1, easy to operate, operating cost is low the present invention relates to the recycling technique of sea cucumber water cooking liquid, it is environmentally protective, it is suitable for
It promotes, and promotes the further genralrlization of Holothurian machining industry.
2, sea cucumber boiling liquid extract is applied in the formula of Skin whitening care cosmetics by the present invention, starts the extraction of sea cucumber water cooking liquid
Object to improve Holothurian machining added value, while can also reduce the pollution to environment, and open in the new application of skin care field
Good economic benefit is created, there is good foreground.
3, the present invention substitutes the chemically synthesized grease generally used in skin care item with natural oil lipid and natural thickener
Class and thickener, small to the irritation of skin, safe to use, environmentally protective, finally avoid in skin care item excessive use has
It is influenced caused by evil synthetics confrontation product quality and consumer health.
Description of the drawings
Fig. 1 is ginsenoside Re's standard curve in embodiment 1；
Fig. 2 is sea cucumber water cooking liquid extract solution DPPH clearance rates in skin care item in embodiment 1；
Fig. 3 is the sea cucumber water cooking liquid extract solution DPPH clearance rates being not added into embodiment 1 in skin care item
Specific implementation mode
It is the specific implementation example of the present invention below, the advantages of for further illustrating the present invention and characteristic, but the present invention
It is not limited by following embodiment.
Embodiment 1
The mass fraction of the Skin whitening care cosmetics each component of the present embodiment liquid extract of boiling containing sea cucumber is respectively sea cucumber boiling
It is 0.14 part of liquid extract, 20 parts of stearic acid, 1.2 parts of triethanolamine, 0.36 part of sodium hydroxide, 0.5 part of hexadecanol, 8 parts of glycerine, right
0.1 part of methyl hydroxybenzoate, 0.05 part of flavoring rose essence, deionized water are to 100 parts.
The preparation method of the sea cucumber water cooking liquid reutilization technology and sea cucumber boiling liquid extract Skin whitening care cosmetics, including with
Lower step：
(1) the sea cucumber boiling liquid extract extracts separating step：
1. then 100L sea cucumber water cooking liquids pass sequentially through micro-filtration using disk centrifugal separator 7000r/min centrifugation 25min removal of impurities
System and ultrafiltration system, wherein micro-filtration membrane material are ceramic membrane (Al2O3, 0.5 μm), ultrafiltration membrane material is ceramic membrane (Al2O3,
0.05 μm), the molecular weight cut coverage of ultrafiltration membrane is 10000Da.Collect efflux.
2. will 1. in gained efflux 5L NAK-9 macroporous absorbent resin circulation absorptions 4h (absorption 1h, in stop 0.5h).
NAK-9 macroporous absorbent resins after absorption are transferred in glass chromatography column, are then eluted with water colourless to eluent, and eluent is abandoned
It goes.
3. eluting 3 times of column volumes of macroporous absorbent resin with the ethanol solution of 10v%, eluent discards.
4. eluting 3 times of column volumes of macroporous absorbent resin with the ethanol solution of 60v%, eluent is collected, solvent is recovered under reduced pressure,
Freeze-drying, obtains sea cucumber boiling liquid extract.
5. measuring saponin(e mass content in sea cucumber boiling liquid extract using vanillic aldehyde-ice acetic acid method.
Currently without commodity selenka standard items, it is standard items that selenka can be replaced with ginsenoside Re, with ginseng
Saponin(e quality (μ g) is that the absorbance value under abscissa, wavelength 537nm is that ordinate draws its standard curve, as shown in Figure 1, obtaining
It is to calculation formula：Y=0.0045x-0.0035, R2=0.9991.
Saponin(e mass content is 34.08% in final measurement sea cucumber boiling liquid extract, and sea cucumber polypeptide mass content is
57.65%, impurity 8.27%
(2) according to formula precise sea cucumber boiling liquid extract 0.014g, stearic acid 2g, triethanolamine 0.12g, hydrogen-oxygen
Change sodium 0.036g, hexadecanol 0.05g, glycerine 0.8g, methyl p-hydroxybenzoate 0.01g, flavoring rose essence 0.005g is spare.
(3) stearic acid, hexadecanol are placed in beaker after being mixed and stirred for uniformly and are put at the ultrasonic wave that frequency is 24kHz
15min is managed, as oil phase；It is 24kHz glycerine, triethanolamine and water to be placed in beaker after being mixed and stirred for uniformly and are put into frequency
Ultrasonication 20min, as water phase, and the sea cucumber boiling liquid extract of precise is added in backward water phase.
(4) by oil phase described in (3) and water phase respectively at 90 DEG C of agitating and heating 25min until observing that oil phase and water phase are equal
It is thoroughly mixed into transparence liquid；While oil phase carries out mechanical agitation, water phase is added in oil phase；Reduce mixture temperature
The methyl p-hydroxybenzoate of a small amount of precise is added to 50 DEG C in degree thereto, and it is mixed that lasting stirring makes it solve homogeneously in
It closes in object；Continue to reduce mixture temperature to 40 DEG C, the flavoring rose essence of weighing is added dropwise thereto, continued mechanical stirring makes rose fragrant
Smart uniform dissolution is in the mixture；Mixture temperature is finally reduced to room temperature, it is 7.8 to be used in combination sodium hydroxide to adjust its pH value, standby
With.
(5) mixture is placed in 100 DEG C of high temperature sterilizations of progress, time 15min in closed container and obtains sea cucumber boiling
Liquid extract Skin whitening care cosmetics.
(6) sea cucumber water cooking liquid extract solution in Skin whitening care cosmetics made from (5) is examined to make the removing of DPPH free radicals
With.It is as follows：
1. accurately weighing ready-made skin care item 3.044g in (5), then the sea cucumber water cooking liquid containing 4.26mg carries in skin care item
Object is taken, 7mL deionized waters are added, i.e., original sea cucumber water cooking liquid extract quality concentration are diluted to 0.5g/L by 2g/L, it will
The mixture moves into centrifuge tube, centrifuges half an hour, takes supernatant to continue vacuum filtration and is extracted to get clear sea cucumber water cooking liquid
Object solution.Repeat above-mentioned experimentation, the sea cucumber water cooking liquid extract quality content for respectively obtaining clarified solution be 0.50g/L,
0.67g/L、1.0g/L、1.6g/L、2.0g/L。
2. preparing the DPPH free radical ethanol solutions of 0.04g/L, refrigeration is kept in dark place.Accurately pipette successively above-mentioned solution and
Each 2mL of ethyl alcohol is protected from light in the same centrifuge tube after standing 30min after mixing, and absorbance is then measured at 517nm
Ac, parallel survey is twice；Accurately pipette successively above-mentioned solution and 1. in each 2mL of sea cucumber water cooking liquid extract solution in it is same from
It in heart pipe, is protected from light after mixing after standing 30min, absorbance A i is measured at 517nm, parallel survey is twice；It is accurate successively to move
Take ethyl alcohol and 1. in each 2mL of sea cucumber water cooking liquid extract solution in the same centrifuge tube, be protected from light standing after mixing
After 30min, absorbance A j is measured at 517nm, parallel survey is twice.
According to formula:Clearance rate=[1- (Ai-Aj)/Ac] × 100% calculates sea cucumber water cooking liquid in Skin whitening care cosmetics and extracts
Object solution is to the clearance rates of DPPH free radicals, and parallel determination 3 times.By the sea cucumber water cooking liquid extract solution in 1., with ethyl alcohol
Make blank control, carries out above-mentioned experiment respectively, and parallel survey is twice.
The sea cucumber water cooking liquid extract solution of different quality concentration in Skin whitening care cosmetics is finally calculated to DPPH free radicals
Clearance rate, as shown in Figure 2.
(7) the sea cucumber water cooking liquid extract solution being not added into skin care item tests the scavenging effect of DPPH free radicals,
It is as follows：0.50g/L, 0.67g/L, 1.01g/L, 2.08g/L, 3.33g/L, 5.07g/L, 10.01g/L are prepared respectively
Sea cucumber water cooking liquid solution of extract.Other steps finally calculate the sea cucumber boiling liquid extract of different quality concentration with (6)
Solution is to the clearance rates of DPPH free radicals, as shown in Figure 3.
By Fig. 2, Fig. 3 it is found that sea cucumber water cooking liquid extract solution has good oxidation resistance, tyrosine can be inhibited
The catalytic activity of enzyme, and then melanin is inhibited to be deposited in epidermis, it is finally reached white-skinned face function.Sea cucumber boiling liquid extract is added
Into Skin whitening care cosmetics, solution D PPH free radical scavenging activities decrease, but still have stronger removing DPPH free radical energy
Power illustrates that the Nature comparison of sea cucumber boiling liquid extract is stablized, is not affected or less affected by the influence of skin care item other compositions, still has very
Good oxidation resistance.
As a result：Selenka is extracted using ultrafiltration system, microfiltration systems and macroreticular resin in sea cucumber water cooking liquid, and is used
Vanillic aldehyde-ice acetic acid method measures selenka content, provides a kind of key technology for sea cucumber water cooking liquid recycling, while will be extra large
Ginseng boiling liquid extract is applied in the preparation process of Skin whitening care cosmetics, and the development in pluralism of Holothurian machining industry can be promoted.
Embodiment 2
A kind of sea cucumber boiling liquid extract Skin whitening care cosmetics, the skin care item are by sea cucumber boiling liquid extract, polyoxyethylene
Bay ether, beeswax, siloxanes, corn oil, vitamin E, methyl p-hydroxybenzoate, glycerine, urea, amino acid, lavender are fragrant
The mass fraction that essence and deionized water are made, and prepare each component needed for the skin care item is respectively sea cucumber boiling liquid extract 0.6
Part, 3 parts of polyoxyethylene laurel ether, 10 parts of beeswax, 5 parts of siloxanes, 5 parts of corn oil, 0.1 part of vitamin E, 5 parts of glycerine, to hydroxyl
0.1 part of methyl benzoate, 3 parts of urea, amino acid 1 part, 0.08 part of lavender oil, deionized water are to 100 parts.
The preparation method of the sea cucumber water cooking liquid reutilization technology and sea cucumber boiling liquid extract Skin whitening care cosmetics, including with
Lower step：
(1) the sea cucumber boiling liquid extract extracts separating step：
1. then 100L sea cucumber water cooking liquids are passed sequentially through micro- using disk centrifugal separator 10000r/min centrifugation 30min removal of impurities
Filter system and ultrafiltration system, wherein micro-filtration membrane material are ceramic membrane (Al2O3, 0.5 μm), ultrafiltration membrane material is ceramic membrane (Al2O3,
0.05 μm), the molecular weight cut coverage of ultrafiltration membrane is 5000Da.Collect efflux.
2. will 1. in gained efflux 5L NAK-9 macroporous absorbent resin circulation absorptions 4h (absorption 1h, in stop 0.5h).
NAK-9 macroporous absorbent resins after absorption are transferred in glass chromatography column, are then eluted with water colourless to eluent, and eluent is abandoned
It goes.
3. eluting 3 times of column volumes of macroporous absorbent resin with the ethanol solution of 15v%, eluent discards.
4. eluting 4 times of column volumes of macroporous absorbent resin with the ethanol solution of 75v%, eluent is collected, solvent is recovered under reduced pressure,
Freeze-drying, obtains sea cucumber boiling liquid extract.
5. using vanillic aldehyde-ice acetic acid method to measure in sea cucumber boiling liquid extract saponin(e mass content as 45.02%, sea cucumber
Peptide masses content is 48.58%, impurity 6.40%.
(2) according to formula precise sea cucumber boiling liquid extract 0.06g, polyoxyethylene laurel ether 0.3g, beeswax 1g, silicon
Oxygen alkane 0.5g, corn oil 0.5g, vitamin E 0.01g, methyl p-hydroxybenzoate 0.01g, glycerine 0.5g, urea 0.3g, amino
Sour 0.1g, lavender oil 0.008g, it is spare.
(3) polyoxyethylene laurel ether, beeswax, siloxanes, corn oil, vitamin E, methyl p-hydroxybenzoate are placed in burning
The ultrasonication 15min that frequency is 24kHz is put into after being mixed and stirred in cup uniformly, as oil phase；By glycerine, urea, ammonia
Base acid and water are placed in beaker be mixed and stirred for uniformly after be put into the ultrasonication 20min that frequency is 24kHz, as water phase,
And the sea cucumber boiling liquid extract of precise is added in backward water phase.
(4) by oil phase described in (3) and water phase respectively at 95 DEG C of agitating and heating 20min until observing that oil phase and water phase are equal
It is thoroughly mixed into transparence liquid；While oil phase carries out mechanical agitation, water phase is added in oil phase；Reduce mixture temperature
The methyl p-hydroxybenzoate of a small amount of precise is added to 45 DEG C in degree thereto, and it is mixed that lasting stirring makes it solve homogeneously in
It closes in object；Continue to reduce mixture temperature to 40 DEG C, the lavender oil of weighing is added dropwise thereto, continued mechanical stirring makes to smoke clothing
Hay-scented essence uniform dissolution is in the mixture；Mixture temperature is finally reduced to room temperature, is used in combination sodium hydroxide to adjust its pH value to be
7.6, it is spare.
(5) mixture is placed in 100 DEG C of high temperature sterilizations of progress, time 15min in closed container and obtains sea cucumber boiling
Liquid extract Skin whitening care cosmetics.
Above-mentioned skin care item can also efficiently solve in addition to whitening effect and leave nucleic acid because nucleic acid metabolism is unsmooth
The gloomy skin that relic is formed, make skin quality be improved significantly, and toxic side effect is not generated to skin.
Sea cucumber boiling liquid extract of the present invention can also be applied to clean skin liquid, clean skin is revealed, cleansing cream, moisture retention liquid,
Moisturizing dew, moisturiser, skin lotion, skin cream, skin cream, shin moisturizer, cream for skin care, face cream, facial cleanser, toner repair liquid,
Eye cream, during a series of Skin whitening care cosmetics of facial mask are formulated.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope of present disclosure, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber, which is characterized in that composition includes：With 100 parts of mass fractions
Meter, wherein sea cucumber boiling liquid extract account for 0.1~1 part, natural oil lipid account for 5~20 parts, natural thickener account for 0~5 part, it is high
Grade alcohol account for 0~5 part, emulsifier account for 1~25 part, preservative account for 0.1~0.5 part, essence account for 0.05~0.5 part, functional addition
Agent accounts for 0~5 part, water surplus；
The group of the sea cucumber boiling liquid extract becomes：30~90wt% of selenka, 5~60wt% of sea cucumber polypeptide, impurity 5~
10wt%；
The preparation method of the sea cucumber boiling liquid extract is：
S11, sea cucumber water cooking liquid pass sequentially through microfiltration systems and ultrafiltration system after centrifugation cleans, and collect efflux；
S12, the efflux obtained with NAK-9 macroporous absorbent resin adsorption steps S11；The absorption resin is eluted with water to washing
De- liquid is colourless, discards eluent；
S13,2~4 times of column volumes of macroporous absorbent resin are eluted with the ethanol solution of 10~20v%, discards eluent；
S14,3~6 times of column volumes of macroporous absorbent resin are eluted with the ethanol solution of 40~100v%, collects eluent；
S15, after organic solvent is evaporated off in the step S14 eluents collected, it is freeze-dried for 24 hours~72h in -70 DEG C~-40 DEG C,
Sea cucumber boiling liquid extract is made；
The natural oil lipid is camellia oil, olive oil, corn oil, rapeseed oil, sesame oil, wheat-germ oil, cocoa butter, coconut
Oil, palm oil, one or more mixing in beeswax, lanolin；
The natural thickener is natural plant starch, gelatin, guar gum, carragheen, Arabic gum, xanthans, algin
One or more mixing；
The higher alcohol be laruyl alcohol, octadecyl alcolol, hexadecanol, lanonol, cholesterol, phytosterol, octyldodecanol one
Kind or a variety of mixing；
The emulsifier is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene 18
Alcohol, polyoxyethylene ether, one or more mixing of Vinlub 73；
The preservative be ethyl-para-hydroxybenzoate, propylparaben, benzyl alcohol, dichloroxylenol, benzyl chloride phenol one
Kind or a variety of mixing；
The functional additive is one or more mixing of vitamin E, urea, amino acid, hyaluronic acid, sheep placenta；
The essence is bouquet type composition or milk flavoured type essence.
2. the Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber according to claim 1, which is characterized in that the natural oil
Class is one or more mixing in corn oil, rapeseed oil, sesame oil, palm oil, beeswax；
The natural thickener is one or more mixing in natural plant starch, gelatin, guar gum, xanthans；
The higher alcohol is one or more mixing in hexadecanol, octadecyl alcolol；
The emulsifier is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether it is one or more mixed
It closes；
The preservative is one or more mixing of ethyl-para-hydroxybenzoate, propylparaben；
The functional additive is one or more mixing in vitamin E, urea, amino acid.
3. the Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber according to claim 1, which is characterized in that the floral type is fragrant
Essence is flavoring rose essence, cream jasmin france or lavender oil.
4. the preparation method of the Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber described in claim 1, which is characterized in that specific step
Suddenly it is：
S1, according to formula weigh respectively sea cucumber boiling liquid extract, natural oil lipid, natural thickener, higher alcohol, emulsifier,
Preservative, essence, functional additive and water, it is spare；
The formula is：In terms of 100 parts of mass fractions, wherein sea cucumber boiling liquid extract accounts for 0.1~1 part, natural oil lipid accounts for
5~20 parts, natural thickener account for 0~5 part, higher alcohol account for 0~5 part, emulsifier account for 1~25 part, preservative account for 0.1~0.5 part,
Essence account for 0.05~0.5 part, functional additive account for 0~5 part, water surplus；
The group of the sea cucumber boiling liquid extract becomes：30~90wt% of selenka, 5~60wt% of sea cucumber polypeptide, impurity 5~
10wt%；
The preparation method of the sea cucumber boiling liquid extract is：
S11, sea cucumber water cooking liquid pass sequentially through microfiltration systems and ultrafiltration system after centrifugation cleans, and collect efflux；
S12, the efflux obtained with NAK-9 macroporous absorbent resin adsorption steps S11；The absorption resin is eluted with water to washing
De- liquid is colourless, discards eluent；
S13,2~4 times of column volumes of macroporous absorbent resin are eluted with the ethanol solution of 10~20v%, discards eluent；
S14,3~6 times of column volumes of macroporous absorbent resin are eluted with the ethanol solution of 40~100v%, collects eluent；
S15, after organic solvent is evaporated off in the step S14 eluents collected, it is freeze-dried for 24 hours~72h in -70 DEG C~-40 DEG C,
Sea cucumber boiling liquid extract is made；
The natural oil lipid is camellia oil, olive oil, corn oil, rapeseed oil, sesame oil, wheat-germ oil, cocoa butter, coconut
Oil, palm oil, one or more mixing in beeswax, lanolin；
The natural thickener is natural plant starch, gelatin, guar gum, carragheen, Arabic gum, xanthans, algin
One or more mixing；
The higher alcohol be laruyl alcohol, octadecyl alcolol, hexadecanol, lanonol, cholesterol, phytosterol, octyldodecanol one
Kind or a variety of mixing；
The emulsifier is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether, polyoxyethylene 18
Alcohol, polyoxyethylene ether, one or more mixing of Vinlub 73；
The preservative be ethyl-para-hydroxybenzoate, propylparaben, benzyl alcohol, dichloroxylenol, benzyl chloride phenol one
Kind or a variety of mixing；
The functional additive is one or more mixing of vitamin E, urea, amino acid, hyaluronic acid, sheep placenta；
The essence is bouquet type composition or milk flavoured type essence；
S2, natural oil lipid, natural thickener, higher alcohol and the emulsifier for weighing step S1 after mixing, with 24kHz's
Frequency ultrasonic wave handles 10~15min, and 90~100 DEG C of 20~30min of agitating and heating obtain oil phase；By functional additive and
Water handles 10~20min after mixing, with the frequency ultrasonic wave of 24kHz, and the sea cucumber water cooking liquid that step S1 is weighed then is added
Extract, 90~100 DEG C of 20~30min of agitating and heating, obtains water phase；
S3, the obtained water phases of step S2 are added in the oil phase, it is stirring while adding；Mixed solution temperature is reduced to 45
~55 DEG C, the preservative that step S1 is weighed is added, is stirred until homogeneous dissolving；Continue to reduce solution temperature to 30~40 DEG C, is added
The essence that step S1 is weighed is stirred until homogeneous dissolving；Solution temperature is finally reduced to room temperature, with sodium hydrate regulator solution pH value
To 7.5~8.5, mixed liquor is obtained；
S4,100 DEG C of 10~15min of sterilization of mixed liquor that step S3 is obtained are protected to get the whitening of the liquid extract of boiling containing sea cucumber
Skin product.
5. the preparation method of the Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber according to claim 4, which is characterized in that institute
It is one or more mixing in corn oil, rapeseed oil, sesame oil, palm oil, beeswax to state natural oil lipid；
The natural thickener is one or more mixing in natural plant starch, gelatin, guar gum, xanthans；
The higher alcohol is one or more mixing in hexadecanol, octadecyl alcolol；
The emulsifier is stearic acid, sodium hydroxide, triethanolamine, siloxanes, polyoxyethylene laurel ether it is one or more mixed
It closes；
The preservative is one or more mixing of ethyl-para-hydroxybenzoate, propylparaben；
The functional additive is one or more mixing in vitamin E, urea, amino acid.
6. the preparation method of the Skin whitening care cosmetics of the liquid extract of boiling containing sea cucumber according to claim 4, which is characterized in that institute
It is flavoring rose essence, cream jasmin france or lavender oil to state bouquet type composition.
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| CN106265422A (en) * | 2016-08-19 | 2017-01-04 | 朱路 | A kind of Epipremnum aureum (Linde et Andre) (Bunting.) Stichopus japonicus skin care item promoting infant hair growth |
| CN107929324B (en) * | 2017-12-08 | 2020-11-13 | 大连工业大学 | Liposome loaded with sea cucumber decoction extract and preparation method thereof |
| CN111303233B (en) * | 2020-03-27 | 2022-11-29 | 中国海洋大学 | Preparation method and application of sea cucumber phytosterol sulfate |
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| CN105029513A (en) * | 2015-08-20 | 2015-11-11 | 大连润伟海参研发科技有限公司 | Method for extracting saponin, polysaccharide, peptide and melanin in wastewater obtained by boiling sea cucumbers |
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| CN104013647A (en) * | 2014-06-18 | 2014-09-03 | 中国科学院过程工程研究所 | Method for extracting holothurian glycosides |
| CN105029513A (en) * | 2015-08-20 | 2015-11-11 | 大连润伟海参研发科技有限公司 | Method for extracting saponin, polysaccharide, peptide and melanin in wastewater obtained by boiling sea cucumbers |
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