CN106883304A - Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus - Google Patents
Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus Download PDFInfo
- Publication number
- CN106883304A CN106883304A CN201710126553.5A CN201710126553A CN106883304A CN 106883304 A CN106883304 A CN 106883304A CN 201710126553 A CN201710126553 A CN 201710126553A CN 106883304 A CN106883304 A CN 106883304A
- Authority
- CN
- China
- Prior art keywords
- hericium erinaceus
- water
- polysaccharide
- precipitation
- mass fraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus, and (1) dries hericium erinaceus fruiting body crushing, degreasing and drying；(2) hot water extraction and ethanol precipitation；(3) precipitation is washed through absolute ethyl alcohol, acetone, absolute ether, and Sevag method removing proteins, dialysis obtains Hericium erinaceus water extraction water-soluble polysaccharide except small-molecule substance；(4) redissolve, ethanol precipitation, obtain heterogeneity Hericium erinaceus water solubility purified polysaccharide component；(5) filter residue after hot water extraction, drying；NaOH/NaBH4Extract, ethanol precipitation；Absolute ethyl alcohol, acetone, absolute ether washing, Sevag method removing proteins, dialysis obtain Hericium erinaceus alkali carries water-soluble polysaccharide except small-molecule substance.The present invention prepares Hericium erinaceus water extraction water solubility and high viscosity alkali carries water-soluble polysaccharide from hericium erinaceus fruiting body, and utilization rate is high；Hericium erinaceus water extraction water-soluble polysaccharide is purified through alcohol grading, obtains the water-soluble multigroup sugar of purifying of Hericium erinaceus of different nature, process is simple, low cost.
Description
Technical field
The invention belongs to natural products preparation field.
Background technology
Hericium erinaceus（Hericium erinaceus）, it is called Hericium erinaceus, hedgehog bacterium, cauliflower bacterium or mountain volt bacterium etc., including monkey
4 kinds of head bacterium, Small acupuncture Hericium erinaceus (Bull. Ex Fr.) Pers, false Hericium erinaceus and Hydnum, are grown on the rotten wood at many broad-leaveds in remote, thickly forested mountains more
With the injury of standing tree, East Asia Region is distributed widely in.Hericium erinaceus early in《Compendium of Materia Medica》In just it is on the books：Hericium erinaceus is mild-natured,
It is sweet, have the function of " relieving the five internal organs, aid digestion ", it is long-standing in traditional Chinese medicine application, and by people be referred to as with " bear's paw,
One of sea cucumber, shark's fin " four big famous dishes equally celebrated for their achievements, have the title of " meat or fish in element ".Hericium erinaceus as dietotherapeutic fungi, with abundant
Nutritive value and medical value, can be used in the diseases such as auxiliary treatment gastric ulcer, indigestion, gastritis, tumour.
Rich in compositions such as polysaccharide, polypeptide and vitamins, wherein hericium erinaceum polysaccharide is one of main active material to Hericium erinaceus.
Modern medicine and pharmaceutical research show that hericium erinaceum polysaccharide has protection digestive system, antitumor and enhance immunity, antioxygen
The physiological function such as change, anti-aging, hypoglycemic and reducing blood lipid, antiviral and antibacterial.
Hericium erinaceum polysaccharide can be extracted from hericium erinaceus fruiting body, mycelium and zymotic fluid and obtained, wherein conduct is natural certainly
The hericium erinaceus fruiting body of right resource is widely used in the extraction of hericium erinaceum polysaccharide.The extracting method of hericium erinaceum polysaccharide includes solvent
（Water, acid, alkali etc.）Extraction method, physical pressure（Microwave, ultrasonic wave）Extraction method, biology（Cellulase, pectase）Extraction method and
The method of various method compound collectings.Wherein, hot water extraction is the most frequently used extracting method, easy to operate with low cost, is had
The characteristics of attribute original beneficial to guarantee polysaccharide, but extraction yield is not high, largely causes the waste of raw material.Therefore to warp
Hericium erinaceus residue after hot water extraction reuse being highly desirable to.The present invention is prepared using the method for hot water extraction first
Hericium erinaceus water extraction water solubility refined polysaccharide, gained filter residue further prepares Hericium erinaceus alkali carries water solubility refined polysaccharide through alkaline process.Institute
Obtain Hericium erinaceus water extraction water solubility refined polysaccharide to be purified using the method for ethanol precipitation, obtain Hericium erinaceus of different nature
Water-soluble purified polysaccharide component.
The content of the invention
Comprehensively prepared and purification process it is an object of the invention to provide heterogeneity polysaccharide in a kind of Hericium erinaceus.
What the present invention was achieved through the following technical solutions.
Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus of the present invention, is comprised the following steps that.
（1）To dry hericium erinaceus fruiting body as raw material, size-reduced machine is crushed and obtains powder；To adding 15 in Hericium erinaceus powder
The ethanol of times quality（Ethanol mass fraction is 95% ~ 100%）24 h are soaked, 4 layers of filtered through gauze collect precipitation, volatilize ethanol, institute
Obtain Hericium erinaceus powder standby.
（2）By step（1）In Hericium erinaceus powder mix with distilled water, weight/volume（g/mL）Than being 1:15~1:30,
The h of boiling waterbath 2, filtered through gauze, the min of 4800 r/min centrifugations 15 are used by extract solution successively, obtain filtrate and filter residue；
Filter residue is extracted again, and extract solution obtains filtrate again through filtered through gauze, the min of 4800 r/min centrifugations 15, merges twice
Gained filtrate is extracted, filtrate and filter residue is obtained and is done following treatment respectively.
A. filtrate is used for the preparation of Hericium erinaceus water extraction water solubility refined polysaccharide, and to Hericium erinaceus water extraction water solubility refined polysaccharide
Purified：
1. gained filtrate is slowly added to the second that mass fraction is 95% toward concentrate through being concentrated under reduced pressure into original volume 1/10 ~ 1/5
Alcohol, until ethanol mass fraction is 80% in system, 20 ~ 25 DEG C of room temperature stands 12 more than h, the r/min of rotating speed 4800 centrifugations 15
Min, collects precipitation.
2. step 1. in precipitation in add absolute ethyl alcohol, acetone and absolute ether, washing by soaking, gained sample successively
Distilled water is added in 65 DEG C of redissolution, protein is removed using Sevag methods；The polysaccharide solution of deproteination matter is concentrated under reduced pressure, will
Concentrate is dialysed（Bag filter is 3500 Da with respect to molecular cut off）, successively with 24 h of flowing running water dialysis, distilled water
Dialyse 36 h；After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus water extraction water solubility refined polysaccharide is obtained.
3. step 2. in Hericium erinaceus water extraction water solubility refined polysaccharide in add distillation water dissolves, weight/volume（g/
mL）Than being 1:15~1:30, be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 10%, in 4 ~ 25 DEG C stand 12
H, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20, collects precipitation and supernatant respectively.
4. step 3. in supernatant in be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 50%,
12 h are stood in 4 ~ 25 DEG C, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant is collected respectively.
5. step 4. in supernatant in be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 60%,
12 h are stood in 4 ~ 25 DEG C, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant is collected respectively.
6. step 5. in supernatant it is slow in add absolute ethyl alcohol until ethanol mass fraction reaches 70% in system,
12 h are stood in 4 ~ 25 DEG C, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant is collected respectively.
7. step 6. in supernatant in be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 80%,
12 h are stood in 4 ~ 25 DEG C, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant is collected respectively.
8. by step, 3., 4., 5., 6. and 7. centrifugation gained precipitates the redissolution that adds water respectively, then through concentrated under reduced pressure, freezing
Heterogeneity Hericium erinaceus water solubility purified polysaccharide component can be respectively obtained after drying.
B. filter residue is used for the preparation of Hericium erinaceus alkali carries water solubility refined polysaccharide：
1. added in gained filter residue（0.1M~0.5M）NaOH/0.01M NaBH4Solution, weight/volume（g/mL）Than being 1:10
~1:12 h are extracted in 30,4 DEG C, extract solution is used into filtered through gauze, the min of 4800 r/min centrifugations 15 successively, collected respectively
Filtrate and filter residue；Filter residue is extracted again, and extract solution collects filter again through filtered through gauze, the min of 4800 r/min centrifugations 15
Liquid；Merge and extract twice gained filtrate, be concentrated under reduced pressure into original volume 1/10 ~ 1/5, being slowly added to mass fraction toward concentrate is
95% ethanol, until ethanol mass fraction is 80%, room temperature in system（20~25 ℃）Stand 12 more than h, 4800 r/min from
The min of the heart 15, collects precipitation.
2. step 1. in precipitation add absolute ethyl alcohol, acetone and absolute ether successively, washing by soaking, gained sample is again
Add distilled water in 70 DEG C of redissolution, protein is removed using Sevag methods；The polysaccharide solution of deproteination matter is concentrated under reduced pressure, will be dense
Contracting liquid is dialysed（Bag filter is 3500 Da with respect to molecular cut off）, it is saturating with 24 h of flowing running water dialysis, distilled water successively
Analyse 36 h；After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus alkali carries water solubility refined polysaccharide is obtained.
Step of the present invention（1）In for soaking the ethanol mass fraction preferably 95% of Hericium erinaceus powder.
Sevag reagents used by Sevag methods deproteination matter of the present invention（N-butanol：Chloroform=1:4）With polysaccharide solution
Volume ratio be 1:3.
The present invention is water-soluble to the comprehensive Hericium erinaceus water extraction for preparing and alkali carries water solubility refined polysaccharide has been carried out substantially
Physicochemical property（Yield, chemical composition analysis, monose composition, apparent viscosity）Measure, and it is water-soluble to determine Hericium erinaceus water extraction
The detection of the yield of refined polysaccharide water-soluble purified polysaccharide component of different nature, neutral sugar content, molecular weight distribution and homogeneity：
（1）Hericium erinaceus water extraction water solubility and alkali carries water solubility refined polysaccharide yield determination：Hericium erinaceus water extraction after will be lyophilized is water-soluble
Property and alkali carries water solubility refined polysaccharide weigh, its yield is calculated with material quality：
A) Hericium erinaceus water extraction water solubility refined polysaccharide yield/%=（Hericium erinaceus water extraction water solubility refined polysaccharide quality/material quality）
×100%。
B) Hericium erinaceus alkali carries water solubility refined polysaccharide yield/%=（Hericium erinaceus alkali carries water solubility refined polysaccharide quality/raw material
Quality）×100%.
（2）Hericium erinaceus water extraction water solubility refined polysaccharide alcohol grading heterogeneity water solubility purified polysaccharide component yield is surveyed
It is fixed：Purified polysaccharide component after will be lyophilized is weighed, based on its yield of Hericium erinaceus water extraction water solubility refined polysaccharide Mass Calculation：
Purified polysaccharide component yield/%=（Purified polysaccharide constituent mass/Hericium erinaceus water extraction water solubility refined polysaccharide quality）×100%.
（3）Hericium erinaceus water extraction is water-soluble and alkali carries water solubility refined polysaccharide basic physical and chemical is analyzed：Using phenolsulfuric acid
Method determines its neutral sugar content, and its glucuronic acid content is determined using sulfate-carbazole, and its albumen is determined using Coomassie Brilliant Blue
Matter content；Monose composition measuring is carried out using chromatography of ions instrument means, and apparent viscosity is surveyed using AERS-G2 rheometers
It is fixed.
（4）The purified polysaccharide component that Hericium erinaceus water extraction water solubility refined polysaccharide is obtained through alcohol grading, neutral sugar content is used
Phend-sulphuric acid is determined, and molecular weight distribution and homogeneity detection use efficient molecular-exclusion chromatography（HPSEC）.
The advantage of the invention is that：It is high that polysaccharide comprehensively extracts yield；Water extraction water solubility refined polysaccharide is by simple and effective
Ethanol precipitation, obtains sugared content and the more good high-purity component of homogeneity；Alkali carries water solubility refined polysaccharide viscosity compared with
It is high.
Specific embodiment
The present invention will be described further by following examples.
Embodiment 1.
To dry hericium erinaceus fruiting body as raw material, size-reduced machine is crushed and obtains powder；To 15 times of addition in Hericium erinaceus powder
Ethanol（Ethanol mass fraction is 95%）24 h are soaked, filtered through gauze collects precipitation, volatilizes ethanol, and gained Hericium erinaceus dry powder is standby
With.Hericium erinaceus powder mixes with distilled water, weight/volume（g/mL）Than being 1:15, the h of boiling waterbath 2, by extract solution successively
With filtered through gauze, the min of 4800 r/min centrifugations 15, filter residue is collected；Filter residue is extracted again, and extract solution is again through gauze mistake
Filter, the min of 4800 r/min centrifugations 15 obtain filtrate, merge and extract twice gained filtrate, and filtrate and filter residue are collected respectively.
Filtrate is used for the preparation of Hericium erinaceus water extraction water solubility refined polysaccharide.Filtrate is past to concentrate through being concentrated under reduced pressure into original volume 1/10 ~ 1/5
The ethanol that mass fraction is 95% is slowly added in liquid, until ethanol mass fraction is 80%, room temperature in system（20~25 ℃）It is quiet
12 more than h are put, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation adds absolute ethyl alcohol, acetone and anhydrous second successively
Ether, washing by soaking, gained sample adds distilled water in 65 DEG C of redissolution, and protein is removed using Sevag methods.Deproteination matter
Polysaccharide solution be concentrated under reduced pressure, concentrate is dialysed（Bag filter is 3500 Da with respect to molecular cut off）, successively with flowing
24 h of running water dialysis, 36 h of distilled water dialysis.After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus water extraction is obtained
Water-soluble refined polysaccharide.Hericium erinaceus water extraction water solubility refined polysaccharide adds distillation water dissolves, weight/volume（g/mL）Than being 1:
15, be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 10%, in 4 DEG C stand 12 h, by above-mentioned alcohol precipitation system
The min of 10000 r/min centrifugations 20, collects precipitation and supernatant respectively.Supernatant is slowly added to absolute ethyl alcohol until system
Middle ethanol mass fraction reaches 50%, and 12 h are stood in 4 DEG C, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20,
Precipitation and supernatant are collected respectively.Supernatant be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 60%, in 4
DEG C 12 h are stood, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant are collected respectively.Supernatant
Liquid be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 70%, in 4 ~ 25 DEG C stand 12 h, by above-mentioned alcohol precipitation body
It is the min of 10000 r/min centrifugations 20, precipitation and supernatant is collected respectively；Supernatant is slowly added to absolute ethyl alcohol until body
Ethanol mass fraction reaches 80% in system, 12 h is stood in 4 ~ 25 DEG C, by the r/min centrifugations 20 of above-mentioned alcohol precipitation system 10000
Min, collects precipitation；The redissolution that adds water respectively is precipitated through alcohol grading gained more than collecting, then can after concentrated under reduced pressure, freeze-drying
Obtain heterogeneity Hericium erinaceus water solubility purified polysaccharide component.Filter residue is used for the preparation of Hericium erinaceus alkali carries water solubility refined polysaccharide.To
Filter residue adds 0.1M NaOH/0.01M NaBH4, weight/volume（g/mL）Than being 1:12 h are extracted in 30,4 DEG C, by extract solution
Filtered through gauze, the min of 4800 r/min centrifugations 15 are used successively, and filtrate and filter residue are collected respectively；Filter residue is extracted again, is extracted
Liquid collects filtrate again through filtered through gauze, the min of 4800 r/min centrifugations 15；Merge and extract twice gained filtrate, decompression
Original volume 1/10 ~ 1/5 is concentrated into, the ethanol that mass fraction is 95% is slowly added to toward concentrate, until ethanol quality in system
Fraction is 80%, room temperature（20~25 ℃）12 more than h are stood, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation according to
Secondary addition absolute ethyl alcohol, acetone and absolute ether, washing by soaking, gained sample add distilled water in 70 DEG C of redissolution, use
Sevag methods remove protein.The polysaccharide solution of deproteination matter is concentrated under reduced pressure, and concentrate is dialysed（Bag filter is relative to be cut
It is 3500 Da to stay molecular weight）, successively with 24 h of flowing running water dialysis, 36 h of distilled water dialysis；After dialysis terminates, will dialyse
Liquid concentration, freeze-drying, obtain Hericium erinaceus alkali carries water solubility refined polysaccharide.Hericium erinaceus water extraction is water-soluble and alkali carries are water-soluble refined
Polysaccharide yield is respectively 4.07% and 1.25%；Neutral sugar content, glucuronic acid content and protein content be respectively 66.00%,
4.14%th, 7.70% and 55.97%, 6.96%, 7.31%；Hericium erinaceus water extraction water solubility refined polysaccharide it is main by fucose, galactolipin and
Glucose group into, and Hericium erinaceus alkali carries water solubility refined polysaccharide it is main by glucose group into；In 0.01 ~ 1000 s-1Shear rate
In the range of, mass fraction be 0.5% Hericium erinaceus water extraction water solubility refined polysaccharide shear rate be 0.01 s-1When reach maximum
The Pa.s of apparent viscosity 0.06, and the Hericium erinaceus alkali carries water solubility refined polysaccharide that mass fraction is 0.5% is 0.01 in shear rate
s-1When reach the Pa.s of maximum apparent viscosity 0.20.Hericium erinaceus water extraction water solubility refined polysaccharide obtained through ethanol precipitation 10%,
50%, 60%, 70% and 80% classification polysaccharide component yield is respectively 20.60%, 29.90%, 29.40%, 16.70%, 8.60%；It is neutral
Sugared content is respectively 61.08%, 73.42%, 72.28%, 65.69%, 75.55%；Show 50%, 60%, 70% He through HPSEC detections
80% alcohol grading component homogeneity is preferable.
Embodiment 2.
To dry hericium erinaceus fruiting body as raw material, size-reduced machine is crushed and obtains powder；To 15 times of addition in Hericium erinaceus powder
Ethanol（Ethanol mass fraction is 95%）24 h are soaked, filtered through gauze collects precipitation, volatilizes ethanol, and gained Hericium erinaceus dry powder is standby
With.Hericium erinaceus powder mixes with distilled water, weight/volume（g/mL）Than being 1:15, the h of boiling waterbath 2, by extract solution successively
With filtered through gauze, the min of 4800 r/min centrifugations 15, filter residue is collected；Filter residue is extracted again, and extract solution is again through gauze mistake
Filter, the min of 4800 r/min centrifugations 15 obtain filtrate, merge and extract twice gained filtrate, and filtrate and filter residue are collected respectively.
Filtrate is used for the preparation of Hericium erinaceus water extraction water solubility refined polysaccharide.Filtrate is past to concentrate through being concentrated under reduced pressure into original volume 1/10 ~ 1/5
The ethanol that mass fraction is 95% is slowly added in liquid, until ethanol mass fraction is 80%, room temperature in system（20~25 ℃）It is quiet
12 more than h are put, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation adds absolute ethyl alcohol, acetone and anhydrous second successively
Ether, washing by soaking, gained sample adds distilled water in 65 DEG C of redissolution, and protein is removed using Sevag methods.Deproteination matter
Polysaccharide solution be concentrated under reduced pressure, concentrate is dialysed（Bag filter is 3500 Da with respect to molecular cut off）, successively with flowing
24 h of running water dialysis, 36 h of distilled water dialysis.After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus water extraction is obtained
Water-soluble refined polysaccharide.Hericium erinaceus water extraction water solubility refined polysaccharide adds distillation water dissolves, weight/volume（g/mL）Than being 1:
15, be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 10%, in 4 DEG C stand 12 h, by above-mentioned alcohol precipitation system
The min of 10000 r/min centrifugations 20, collects precipitation and supernatant respectively.Supernatant is slowly added to absolute ethyl alcohol until system
Middle ethanol mass fraction reaches 50%, and 12 h are stood in 4 DEG C, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20,
Precipitation and supernatant are collected respectively.Supernatant be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 60%, in 4
DEG C 12 h are stood, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant are collected respectively.Supernatant
Liquid be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 70%, in 4 ~ 25 DEG C stand 12 h, by above-mentioned alcohol precipitation body
It is the min of 10000 r/min centrifugations 20, precipitation and supernatant is collected respectively；Supernatant is slowly added to absolute ethyl alcohol until body
Ethanol mass fraction reaches 80% in system, 12 h is stood in 4 ~ 25 DEG C, by the r/min centrifugations 20 of above-mentioned alcohol precipitation system 10000
Min, collects precipitation；The redissolution that adds water respectively is precipitated through alcohol grading gained more than collecting, then can after concentrated under reduced pressure, freeze-drying
Obtain heterogeneity Hericium erinaceus water solubility purified polysaccharide component.Filter residue is used for the preparation of Hericium erinaceus alkali carries water solubility refined polysaccharide.To
Filter residue adds 0.5 M NaOH/0.01M NaBH4, weight/volume is 1:10（g/mL）, 12 h are extracted in 4 DEG C, will extract
Liquid uses filtered through gauze, the min of 4800 r/min centrifugations 15 successively, and filtrate and filter residue are collected respectively；Filter residue is extracted again, is carried
Liquid is taken again through filtered through gauze, the min of 4800 r/min centrifugations 15, collects filtrate；Merge and extract twice gained filtrate, subtract
Pressure is concentrated into original volume 1/10 ~ 1/5, the ethanol that mass fraction is 95% is slowly added to toward concentrate, until ethanol matter in system
Amount fraction is 80%, room temperature（20~25 ℃）12 more than h are stood, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation
Absolute ethyl alcohol, acetone and absolute ether, washing by soaking are added successively, and gained sample adds distilled water in 70 DEG C of redissolution, uses
Sevag methods remove protein.The polysaccharide solution of deproteination matter is concentrated under reduced pressure, and concentrate is dialysed（Bag filter is relative to be cut
It is 3500 Da to stay molecular weight）, successively with 24 h of flowing running water dialysis, 36 h of distilled water dialysis；After dialysis terminates, will dialyse
Liquid concentration, freeze-drying, obtain Hericium erinaceus alkali carries water solubility refined polysaccharide.Hericium erinaceus water extraction is water-soluble and alkali carries are water-soluble refined
Polysaccharide yield is respectively 4.07% and 3.78%；Neutral sugar content, glucuronic acid content and protein content be respectively 66.00%,
7.70%th, 4.14% and 92.97%, 7.72%, 2.16%；Hericium erinaceus water extraction water solubility refined polysaccharide it is main by fucose, galactolipin and
Glucose group into, and Hericium erinaceus alkali carries water solubility refined polysaccharide it is main by glucose group into；In 0.01 ~ 1000 s-1Shear rate
In the range of, mass fraction be 0.5% Hericium erinaceus water extraction water solubility refined polysaccharide shear rate be 0.01 s-1When reach maximum
The Pa.s of apparent viscosity 0.06, mass fraction be 0.5% Hericium erinaceus alkali carries water solubility refined polysaccharide shear rate be 0.01 s-1
When reach the Pa.s of maximum apparent viscosity 32.57.Hericium erinaceus water extraction water solubility refined polysaccharide obtained through ethanol precipitation 10%, 50%,
60%, 70% and 80% classification polysaccharide component yield is respectively 20.60%, 29.90%, 29.40%, 16.70%, 8.60%；Neutral sugar contains
Amount is respectively 61.08%, 73.42%, 72.28%, 65.69%, 75.55%；Show 50%, 60%, 70% and 80% second through HPSEC detections
Alcohol grading purification polysaccharide group homogeneity is preferable.
Embodiment 3.
To dry hericium erinaceus fruiting body as raw material, size-reduced machine is crushed and obtains powder；To 15 times of addition in Hericium erinaceus powder
Ethanol（Ethanol mass fraction is 95%）24 h are soaked, filtered through gauze collects precipitation, volatilizes ethanol, and gained Hericium erinaceus dry powder is standby
With.Hericium erinaceus powder mixes with distilled water, weight/volume（g/mL）Than being 1:30, the h of boiling waterbath 2, by extract solution successively
With filtered through gauze, the min of 4800 r/min centrifugations 15, filter residue is collected；Filter residue is extracted again, and extract solution is again through gauze mistake
Filter, the min of 4800 r/min centrifugations 15 obtain filtrate, merge and extract twice gained filtrate, and filtrate and filter residue are collected respectively.
Filtrate is used for the preparation of Hericium erinaceus water extraction water solubility refined polysaccharide.Filtrate is past to concentrate through being concentrated under reduced pressure into original volume 1/10 ~ 1/5
The ethanol that mass fraction is 95% is slowly added in liquid, until ethanol mass fraction is 80%, room temperature in system（20~25 ℃）It is quiet
12 more than h are put, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation adds absolute ethyl alcohol, acetone and anhydrous second successively
Ether, washing by soaking, gained sample adds distilled water in 65 DEG C of redissolution, and protein is removed using Sevag methods.Deproteination matter
Polysaccharide solution be concentrated under reduced pressure, concentrate is dialysed（Bag filter is 3500 Da with respect to molecular cut off）, successively with flowing
24 h of running water dialysis, 36 h of distilled water dialysis.After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus water extraction is obtained
Water-soluble refined polysaccharide.Hericium erinaceus water extraction water solubility refined polysaccharide adds distillation water dissolves, weight/volume（g/mL）Than being 1:
15, be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 10%, in 4 DEG C stand 12 h, by above-mentioned alcohol precipitation system
The min of 10000 r/min centrifugations 20, collects precipitation and supernatant respectively.Supernatant is slowly added to absolute ethyl alcohol until system
Middle ethanol mass fraction reaches 50%, and 12 h are stood in 4 DEG C, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20,
Precipitation and supernatant are collected respectively.Supernatant be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 60%, in 4
DEG C 12 h are stood, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20, precipitation and supernatant are collected respectively.Supernatant
Liquid be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 70%, in 4 ~ 25 DEG C stand 12 h, by above-mentioned alcohol precipitation body
It is the min of 10000 r/min centrifugations 20, precipitation and supernatant is collected respectively；Supernatant is slowly added to absolute ethyl alcohol until body
Ethanol mass fraction reaches 80% in system, 12 h is stood in 4 ~ 25 DEG C, by the r/min centrifugations 20 of above-mentioned alcohol precipitation system 10000
Min, collects precipitation；The redissolution that adds water respectively is precipitated through alcohol grading gained more than collecting, then can after concentrated under reduced pressure, freeze-drying
Obtain heterogeneity Hericium erinaceus water solubility purified polysaccharide component.Filter residue is used for the preparation of Hericium erinaceus alkali carries water solubility refined polysaccharide.To
Filter residue adds 0.1M NaOH/0.01M NaBH4, weight/volume（g/mL）Than being 1:12 h are extracted in 30,4 DEG C, by extract solution
Filtered through gauze, the min of 4800 r/min centrifugations 15 are used successively, and filtrate and filter residue are collected respectively；Filter residue is extracted again, is extracted
Liquid collects filtrate again through filtered through gauze, the min of 4800 r/min centrifugations 15；Merge and extract twice gained filtrate, decompression
Original volume 1/10 ~ 1/5 is concentrated into, the ethanol that mass fraction is 95% is slowly added to toward concentrate, until ethanol quality in system
Fraction is 80%, room temperature（20~25 ℃）12 more than h are stood, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation according to
Secondary addition absolute ethyl alcohol, acetone and absolute ether, washing by soaking, gained sample add distilled water in 70 DEG C of redissolution, use
Sevag methods remove protein.The polysaccharide solution of deproteination matter is concentrated under reduced pressure, and concentrate is dialysed（Bag filter is relative to be cut
It is 3500 Da to stay molecular weight）, successively with 24 h of flowing running water dialysis, 36 h of distilled water dialysis；After dialysis terminates, will dialyse
Liquid concentration, freeze-drying, obtain Hericium erinaceus water extraction alkali solubility refined polysaccharide.Hericium erinaceus water extraction is water-soluble and alkali carries are water-soluble refined
Polysaccharide yield is respectively 3.92% and 0.80%；Neutral sugar content, glucuronic acid content and protein content be respectively 69.68%,
7.53%th, 2.18% and 58.17%, 7.03%, 5.75%；Hericium erinaceus water solubility refined polysaccharide is main by fucose, galactolipin and grape
Sugar composition, and Hericium erinaceus alkali carries water solubility refined polysaccharide it is main by glucose group into；In 0.01 ~ 1000 s-1Range of shear rate
It is interior, mass fraction be 0.5% Hericium erinaceus water extraction water solubility refined polysaccharide shear rate be 0.01 s-1When reach it is maximum apparent
The Pa.s of viscosity 0.05, mass fraction be 0.5% Hericium erinaceus alkali carries water solubility refined polysaccharide shear rate be 0.01 s-1Shi Da
It is 0.30 Pa.s to maximum apparent viscosity.Hericium erinaceus water extraction water solubility refined polysaccharide obtained through ethanol precipitation 10%, 50%,
60%, 70% and 80% classification polysaccharide component yield is respectively 23.20%, 30.40%, 28.70%, 19.50%, 10.60%；Neutral sugar contains
Amount is respectively 78.81%, 77.89%, 77.04%, 69.40%, 70.32%；Show 50%, 60%, 70% and 80% second through HPSEC detections
Alcohol grading purification polysaccharide group homogeneity is preferable.
Embodiment 4.
To dry hericium erinaceus fruiting body as raw material, size-reduced machine is crushed and obtains powder；To 15 times of addition in Hericium erinaceus powder
Ethanol（Ethanol mass fraction is 95%）24 h are soaked, filtered through gauze collects precipitation, volatilizes ethanol, and gained Hericium erinaceus dry powder is standby
With.Hericium erinaceus powder mixes with distilled water, bulking value（g/mL）Than being 1:30, the h of boiling waterbath 2, extract solution is used successively
Filtered through gauze, the min of 4800 r/min centrifugations 15, collect filter residue；Filter residue is extracted again, extract solution again through filtered through gauze,
The min of 4800 r/min centrifugations 15 obtains filtrate, merges and extract twice gained filtrate, and filtrate and filter residue are collected respectively.Filtrate
For the preparation of Hericium erinaceus water extraction water solubility refined polysaccharide.Filtrate through being concentrated under reduced pressure into original volume 1/10 ~ 1/5, toward concentrate
The ethanol that mass fraction is 95% is slowly added to, until ethanol mass fraction is 80%, room temperature in system（20~25 ℃）Stand 12
More than h, the min of 4800 r/min centrifugations 15 collect precipitation.Precipitation adds absolute ethyl alcohol, acetone and absolute ether, leaching successively
Foam washing is washed, and gained sample adds distilled water in 65 DEG C of redissolution, and protein is removed using Sevag methods.The polysaccharide of deproteination matter
Solution decompression is concentrated, and concentrate is dialysed（Bag filter is 3500 Da with respect to molecular cut off）, successively with flowing running water
Dialyse 24 h, 36 h of distilled water dialysis.After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus water extraction is obtained water-soluble
Refined polysaccharide.Hericium erinaceus water extraction water solubility refined polysaccharide adds distillation water dissolves, weight/volume（g/mL）Than being 1:15, slowly
Add absolute ethyl alcohol until system in ethanol mass fraction reach 10%, in 4 DEG C stand 12 h, by above-mentioned alcohol precipitation system 10000
The min of r/min centrifugations 20, collects precipitation and supernatant respectively.Supernatant is slowly added to absolute ethyl alcohol up to ethanol in system
Mass fraction reaches 50%, and 12 h are stood in 4 DEG C, and the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20 is received respectively
Collection precipitation and supernatant.Supernatant be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 60%, in 4 DEG C standing
12 h, by the above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20, collect precipitation and supernatant respectively.Supernatant is slow
Add absolute ethyl alcohol until system in ethanol mass fraction reach 70%, in 4 ~ 25 DEG C stand 12 h, by above-mentioned alcohol precipitation system
The min of 10000 r/min centrifugations 20, collects precipitation and supernatant respectively；Supernatant is slowly added to absolute ethyl alcohol until system
Middle ethanol mass fraction reaches 80%, 12 h is stood in 4 ~ 25 DEG C, by the r/min centrifugations 20 of above-mentioned alcohol precipitation system 10000
Min, collects precipitation；The redissolution that adds water respectively is precipitated through alcohol grading gained more than collecting, then can after concentrated under reduced pressure, freeze-drying
Obtain heterogeneity Hericium erinaceus water solubility purified polysaccharide component.Filter residue is used for the preparation of Hericium erinaceus alkali carries water solubility refined polysaccharide.To
Filter residue adds 0.5 M NaOH/0.01M NaBH4, weight/volume（g/mL）Than being 1:12 h are extracted in 10,4 DEG C, will be extracted
Liquid uses filtered through gauze, the min of 4800 r/min centrifugations 15 successively, and filtrate and filter residue are collected respectively；Filter residue is extracted again, is carried
Liquid is taken again through filtered through gauze, the min of 4800 r/min centrifugations 15, collects filtrate；Merge and extract twice gained filtrate, subtract
Pressure is concentrated into original volume 1/10 ~ 1/5, the ethanol that mass fraction is 95% is slowly added to toward concentrate, until ethanol matter in system
Amount fraction is 80%, room temperature（20~25 ℃）12 more than h are stood, the min of 4800 r/min centrifugations 15 collects precipitation.Precipitation
Absolute ethyl alcohol, acetone and absolute ether, washing by soaking are added successively, and gained sample adds distilled water in 70 DEG C of redissolution, uses
Sevag methods remove protein.The polysaccharide solution of deproteination matter is concentrated under reduced pressure, and concentrate is dialysed（Bag filter is relative to be cut
It is 3500 Da to stay molecular weight）, successively with 24 h of flowing running water dialysis, 36 h of distilled water dialysis；After dialysis terminates, will dialyse
Liquid concentration, freeze-drying, obtain Hericium erinaceus alkali carries water solubility refined polysaccharide.Hericium erinaceus water extraction is water-soluble and alkali carries are water-soluble refined
Polysaccharide yield is respectively 3.92% and 1.05%；Neutral sugar content, glucuronic acid content and protein content be respectively 69.68%,
7.53%th, 2.18% and 63.28%, 8.66%, 8.38%；Hericium erinaceus water extraction water solubility refined polysaccharide it is main by fucose, galactolipin and
Glucose group into, and Hericium erinaceus alkali carries water solubility refined polysaccharide it is main by glucose group into；In 0.01 ~ 1000 s-1Shear rate
In the range of, mass fraction be 0.5% Hericium erinaceus water extraction water solubility refined polysaccharide shear rate be 0.01 s-1When reach maximum
The Pa.s of apparent viscosity 0.05, mass fraction be 0.5% Hericium erinaceus alkali carries water solubility refined polysaccharide shear rate be 0.01 s-1
When reach maximum apparent viscosity for 9.40 Pa.s.Hericium erinaceus water extraction water solubility refined polysaccharide obtained through ethanol precipitation 10%,
50%, 60%, 70% and 80% classification polysaccharide component yield is respectively 23.20%, 30.40%, 28.70%, 19.50%, 10.60%；It is neutral
Sugared content is respectively 78.81%, 77.89%, 77.04%, 69.40%, 70.32%；Show 50%, 60%, 70% He through HPSEC detections
80% alcohol grading purified polysaccharide group homogeneity is preferable.
Claims (3)
1. heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus, it is characterized in that step is as follows：
（1）To dry hericium erinaceus fruiting body as raw material, size-reduced machine is crushed and obtains powder；To 15 times of matter of addition in Hericium erinaceus powder
The mass fraction of amount soaks 24 h for 95% ~ 100% ethanol, and 4 layers of filtered through gauze collect precipitation, volatilize ethanol, gained Hericium erinaceus
Powder is standby；
（2）By step（1）In Hericium erinaceus powder mix with distilled water, weight/volume（g/mL）Than being 1:15~1:30, boiling water
2 h are extracted in bath, and extract solution is used into filtered through gauze, the min of 4800 r/min centrifugations 15 successively, obtain filtrate and filter residue；Filter residue
Extract again, extract solution obtains filtrate again through filtered through gauze, the min of 4800 r/min centrifugations 15, and merging is extracted twice
Gained filtrate, obtains filtrate and filter residue and does following treatment respectively：
A. the treatment of filtrate：
1. gained filtrate is slowly added to the second that mass fraction is 95% toward concentrate through being concentrated under reduced pressure into original volume 1/10 ~ 1/5
Alcohol, until ethanol mass fraction is 80% in system, 20 ~ 25 DEG C of standing 12 more than h, the r/min of rotating speed 4800 centrifugations 15 of room temperature
Min, collects precipitation；
2. step 1. in precipitation in add absolute ethyl alcohol, acetone and absolute ether successively, washing by soaking, gained sample adds again
Enter distilled water in 65 DEG C of redissolution, protein is removed using Sevag methods；The polysaccharide solution of deproteination matter is concentrated under reduced pressure, and will concentrate
Liquid is dialysed with the bag filter that molecular cut off relatively is 3500 Da, successively with 24 h of flowing running water dialysis, distilled water dialysis 36
h；After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus water extraction water solubility refined polysaccharide is obtained；
3. step 2. in Hericium erinaceus water extraction water solubility refined polysaccharide in add distillation water dissolves, weight/volume（g/mL）Than
It is 1:15~1:30, be slowly added to absolute ethyl alcohol until system in ethanol mass fraction reach 10%, in 4 ~ 25 DEG C stand 12 h, will
The above-mentioned min of 10000 r/min centrifugations of alcohol precipitation system 20, collects precipitation and supernatant respectively；
4. step 3. in supernatant in be slowly added to absolute ethyl alcohol until ethanol mass fraction reaches 50% in system, in 4 ~
25 DEG C of 12 h of standing, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, collect precipitation and supernatant respectively；
5. step 4. in supernatant in be slowly added to absolute ethyl alcohol until ethanol mass fraction reaches 60% in system, in 4 ~
25 DEG C of 12 h of standing, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, collect precipitation and supernatant respectively；
6. step 5. in supernatant it is slow in add absolute ethyl alcohol until ethanol mass fraction reaches 70% in system, in 4 ~
25 DEG C of 12 h of standing, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, collect precipitation and supernatant respectively；
7. step 6. in supernatant in be slowly added to absolute ethyl alcohol until ethanol mass fraction reaches 80% in system, in 4 ~
25 DEG C of 12 h of standing, by the min of 10000 r/min centrifugations of alcohol precipitation system 20, collect precipitation and supernatant respectively；
8. by step, 3., 4., 5., 6. and 7. centrifugation gained precipitates the redissolution that adds water respectively, then through concentrated under reduced pressure, freeze-drying
After can respectively heterogeneity Hericium erinaceus water solubility purified polysaccharide component；
B. the treatment of filter residue：
1. to addition 0.1M ~ 0.5MNaOH/0.01M NaBH in gained filter residue4Solution, weight/volume（g/mL）Than being 1:10~1:
12 h are extracted in 30,4 DEG C, extract solution is used into filtered through gauze, the min of 4800 r/min centrifugations 15 successively, filter is collected respectively
Liquid and filter residue；Filter residue is extracted again, and extract solution collects filtrate again through filtered through gauze, the min of 4800 r/min centrifugations 15；
Merge and extract twice gained filtrate, be concentrated under reduced pressure into original volume 1/10 ~ 1/5, it is 95% that mass fraction is slowly added to toward concentrate
Ethanol, until ethanol mass fraction is 80% in system, 20 ~ 25 DEG C of room temperature standing 12 more than h, 4800 r/min centrifugation 15
Min, collects precipitation；
2. step 1. in precipitation add absolute ethyl alcohol, acetone and absolute ether successively, washing by soaking, gained sample is added
Distilled water removes protein in 70 DEG C of redissolution using Sevag methods；The polysaccharide solution of deproteination matter is concentrated under reduced pressure, by concentrate
Dialysed with the bag filter that relative molecular cut off is 3500 Da, successively with 24 h of flowing running water dialysis, distilled water dialysis 36
h；After dialysis terminates, by dialyzate concentration, freeze-drying, Hericium erinaceus alkali carries water solubility refined polysaccharide is obtained.
2. heterogeneity polysaccharide is comprehensively prepared and purification process in Hericium erinaceus according to claim 1, it is characterized in that step
（1）In for soak Hericium erinaceus powder ethanol mass fraction be 95%.
3. heterogeneity polysaccharide is comprehensively prepared and purification process in Hericium erinaceus according to claim 1, it is characterized in that described
Sevag method deproteination matter used by the volume ratio of Sevag reagents and polysaccharide solution be 1:3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710126553.5A CN106883304A (en) | 2017-03-06 | 2017-03-06 | Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710126553.5A CN106883304A (en) | 2017-03-06 | 2017-03-06 | Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106883304A true CN106883304A (en) | 2017-06-23 |
Family
ID=59179101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710126553.5A Pending CN106883304A (en) | 2017-03-06 | 2017-03-06 | Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106883304A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619486A (en) * | 2018-08-13 | 2018-10-09 | 常同喜 | It is a kind of to treat dermopathic ointment and preparation method thereof |
| CN110343190A (en) * | 2019-08-21 | 2019-10-18 | 福建农林大学 | A kind of synchronous method for extracting hericium erinaceum polysaccharide and protein |
| CN110903408A (en) * | 2019-11-01 | 2020-03-24 | 福州福德恩生物科技有限公司 | Preparation method of hericium erinaceus polysaccharide protein extract |
| CN111892663A (en) * | 2020-07-22 | 2020-11-06 | 湖北民族大学 | Hericium erinaceus polysaccharide and preparation method and application thereof |
| CN112062866A (en) * | 2020-07-22 | 2020-12-11 | 湖北民族大学 | Hericium erinaceus selenium-rich polysaccharide and preparation method and application thereof |
| CN112125979A (en) * | 2020-08-25 | 2020-12-25 | 福州康来生物科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN113336868A (en) * | 2021-06-10 | 2021-09-03 | 吉林农业大学 | Hericium erinaceus mycelium polysaccharide with effect of preventing and treating senile dementia and preparation method thereof |
| CN113892635A (en) * | 2021-11-15 | 2022-01-07 | 上海市农业科学院 | Method for improving water extract yield of hericium erinaceus through low-temperature treatment |
| CN114209712A (en) * | 2021-11-24 | 2022-03-22 | 常山豪锋生物科技有限公司 | Application of hericium erinaceus polysaccharide in protection of CTX-induced liver injury and preparation method |
| CN116622000A (en) * | 2023-05-12 | 2023-08-22 | 南昌大学 | Preparation process for extracting active soluble polysaccharide by using agrocybe cylindracea |
| CN116649576A (en) * | 2023-06-14 | 2023-08-29 | 深圳市吉迩科技有限公司 | Nicotine buccal product containing antibacterial and antioxidant composition and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861636A (en) * | 2005-05-10 | 2006-11-15 | 上海市农业科学院 | Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose |
| CN101597342A (en) * | 2009-07-14 | 2009-12-09 | 哈尔滨众生北药生物工程有限公司 | From Hericium erinaceus, extract method of water-soluble beta dextran and products thereof |
| CN104059160A (en) * | 2014-06-25 | 2014-09-24 | 上海市农业科学院 | Hericium erinaceus cell wall polysaccharide and preparation method thereof |
-
2017
- 2017-03-06 CN CN201710126553.5A patent/CN106883304A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861636A (en) * | 2005-05-10 | 2006-11-15 | 上海市农业科学院 | Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose |
| CN101597342A (en) * | 2009-07-14 | 2009-12-09 | 哈尔滨众生北药生物工程有限公司 | From Hericium erinaceus, extract method of water-soluble beta dextran and products thereof |
| CN104059160A (en) * | 2014-06-25 | 2014-09-24 | 上海市农业科学院 | Hericium erinaceus cell wall polysaccharide and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 唐川等: ""可溶性固形物含量对制备猴头菇子实体多糖的理化性质及免疫活性的影响"", 《食用菌学报》 * |
| 林华娟等: ""猴头菇子实体碱溶性葡聚糖的分离纯化"", 《食用菌学报》 * |
| 林永成等: "《海洋微生物及其代谢产物》", 31 January 2003, 化学工业出版社 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619486A (en) * | 2018-08-13 | 2018-10-09 | 常同喜 | It is a kind of to treat dermopathic ointment and preparation method thereof |
| CN108619486B (en) * | 2018-08-13 | 2021-12-24 | 常同喜 | Ointment for treating skin diseases and preparation method thereof |
| CN110343190A (en) * | 2019-08-21 | 2019-10-18 | 福建农林大学 | A kind of synchronous method for extracting hericium erinaceum polysaccharide and protein |
| CN110903408A (en) * | 2019-11-01 | 2020-03-24 | 福州福德恩生物科技有限公司 | Preparation method of hericium erinaceus polysaccharide protein extract |
| CN112062866B (en) * | 2020-07-22 | 2022-09-13 | 湖北民族大学 | Hericium erinaceus selenium-rich polysaccharide and preparation method and application thereof |
| CN111892663A (en) * | 2020-07-22 | 2020-11-06 | 湖北民族大学 | Hericium erinaceus polysaccharide and preparation method and application thereof |
| CN112062866A (en) * | 2020-07-22 | 2020-12-11 | 湖北民族大学 | Hericium erinaceus selenium-rich polysaccharide and preparation method and application thereof |
| CN111892663B (en) * | 2020-07-22 | 2022-10-25 | 湖北民族大学 | Hericium erinaceus polysaccharide and preparation method and application thereof |
| CN112125979A (en) * | 2020-08-25 | 2020-12-25 | 福州康来生物科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN113336868A (en) * | 2021-06-10 | 2021-09-03 | 吉林农业大学 | Hericium erinaceus mycelium polysaccharide with effect of preventing and treating senile dementia and preparation method thereof |
| CN113892635A (en) * | 2021-11-15 | 2022-01-07 | 上海市农业科学院 | Method for improving water extract yield of hericium erinaceus through low-temperature treatment |
| CN114209712A (en) * | 2021-11-24 | 2022-03-22 | 常山豪锋生物科技有限公司 | Application of hericium erinaceus polysaccharide in protection of CTX-induced liver injury and preparation method |
| CN116622000A (en) * | 2023-05-12 | 2023-08-22 | 南昌大学 | Preparation process for extracting active soluble polysaccharide by using agrocybe cylindracea |
| CN116649576A (en) * | 2023-06-14 | 2023-08-29 | 深圳市吉迩科技有限公司 | Nicotine buccal product containing antibacterial and antioxidant composition and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106883304A (en) | Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus | |
| CN106117387B (en) | A kind of low molecular weight tremella polysaccharides and the preparation method and application thereof | |
| CN101461514B (en) | Bitter melon extract preparation method | |
| CN103159864B (en) | Five kinds of polysaccharide that a kind of separation purification method of lycium ruthenicum polysaccharide and separation obtain | |
| CN102746412B (en) | Method for extracting momordica charantiap polysaccharide | |
| CN105037572B (en) | A kind of method that burdock polysaccharide is extracted from radix bardanae | |
| CN114014946B (en) | Preparation method of yellow tea polysaccharide and application of yellow tea polysaccharide prepared by preparation method in preserved meat | |
| CN104292352B (en) | A kind of Cortex Eucommiae fine powder, eucommia bark polycose and gutta-percha coproduction extraction separation method | |
| CN102234671B (en) | Method for extracting polysaccharide from longan pulp | |
| CN106832030A (en) | The method that active polysaccharide is extracted from marine alga | |
| CN101508730B (en) | Extract method for lectin of leguminous plants | |
| CN108359022B (en) | Preparation method of lentinan | |
| CN101186628A (en) | Method for extracting naringin and pectine from shaddock peel | |
| CN104558115A (en) | Antioxidant polypeptide with Raja porosa meat protein as well as preparation method and application of antioxidant polypeptide | |
| CN103980372A (en) | Method for concurrently preparing low viscosity semen plantaginis polysaccharide and high viscosity semen plantaginis polysaccharide | |
| CN103848923A (en) | Method for extracting lentinan by taking waste mushroom dregs as raw material | |
| CN103275237B (en) | Preparation method and application of eggplant branch polysaccharide | |
| CN105769727A (en) | Whitening skin-care product containing sea cucumber decoction extract and preparation method of whitening skin-care product | |
| CN103864956A (en) | Method for extracting pectin from eggplant fruits | |
| CN106432524A (en) | Technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth | |
| CN106923350B (en) | Method for preparing water-soluble dietary fiber from corn stigma | |
| CN112442136A (en) | Method for extracting functional components from tremella | |
| CN106167531B (en) | A kind of method of enzyme process assisted extraction longan polysaccharide | |
| CN103864951A (en) | Preparation method and application of hematoxylon polysaccharide | |
| CN105601948B (en) | A kind of method that plantago asiatica linne polyoses viscosity is reduced using tea polyphenols |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170623 |