CN101461514B - Bitter melon extract preparation method - Google Patents

Bitter melon extract preparation method Download PDF

Info

Publication number
CN101461514B
CN101461514B CN2009100009359A CN200910000935A CN101461514B CN 101461514 B CN101461514 B CN 101461514B CN 2009100009359 A CN2009100009359 A CN 2009100009359A CN 200910000935 A CN200910000935 A CN 200910000935A CN 101461514 B CN101461514 B CN 101461514B
Authority
CN
China
Prior art keywords
balsam pear
acid
enzymolysis
bitter melon
extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN2009100009359A
Other languages
Chinese (zh)
Other versions
CN101461514A (en
Inventor
韩金光
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hainan standard biological Polytron Technologies Inc
Original Assignee
韩金光
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 韩金光 filed Critical 韩金光
Priority to CN2009100009359A priority Critical patent/CN101461514B/en
Publication of CN101461514A publication Critical patent/CN101461514A/en
Application granted granted Critical
Publication of CN101461514B publication Critical patent/CN101461514B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses a balsam pear extract and preparation thereof. The extract liquid is made from extraction using fruit of cucurbit balsam pear as raw material. The extraction process includes using water as the solvent, and extracting the balsam pear extract liquid by zymohydrolysis, ultrasonic processing or the like. The balsam pear extract liquid is in light yellow or yellow, contains active substance such as balsam pear saponins, balsam pear polypeptides and active polysaccharide. The process is simple and fast, easy for industrial production, with nutritious extract, without environment pollution, and having low cost. The balsam pear extract has evident function of blood glucose and blood lipid lowering.

Description

The preparation method of Bitter Melon P.E
Technical field
The present invention relates to a kind of food, health products raw material and preparation method thereof, the particularly preparation method of Bitter Melon P.E.
Background technology
Balsam pear is called bright and beautiful lichee, toad, leprosy grape, balsam pear, red sheep, it is the climing property of Curcurbitaceae Momordica herbaceous plant, the balsam pear bitter taste is cold, have clearing heat and detoxicating, nourish strong, hypoglycemic, anti-sudden change, antitumor and improve effect such as body immunity, be one of melon vegetables of liking of people.
Chinese patent literature CN1209155C discloses a kind of preparation method who contains the balsam pear preparation of hypoglycemic composition, be that balsam pear is gone to handle with pectase, cellulase or hemicellulase behind the seed defibrination, through filtering and centrifugal removal pulp residue, separate impervious canescence insoluble matter A through micro-filtration again, clear liquid passes through nanofiltration separation again, obtains B component.Preparation contains saponin(e, albumen, polyphenol oxidase, is used for hypoglycemic.
Chinese patent literature CN101147537A discloses a kind of extracting method of bitter melon polysaccharide, earlier balsam pear powder is purified with ethanol, the plain enzyme of reprocess fibre is handled, filtered filtration residue adds water ultrasonic wave heat treated, filtrate with neutral proteinase handle that the back is centrifugal, dialysis, concentrate, alcohol precipitation, centrifugal final vacuum drying or freeze-drying get the bitter melon polysaccharide goods.
Said method, the composition of extraction is more single, and many useful compositions such as polypeptide fail to keep, and complex process, and recovery rate and efficient are on the low side.
Summary of the invention
The present invention is directed to the unicity of existing extraction balsam pear single component, having proposed a kind of is that raw material extracts the Bitter Melon P.E that gets with the balsam pear.
Bitter Melon P.E of the present invention is that the fruit with the cucurbitaceous plant balsam pear is that its main component of a kind of balsam pear extract that raw material extracts is momordica saponins, micromolecule polypeptide and active polysaccharide.
Bitter Melon P.E of the present invention can be for being faint yellow or yellow liquid, and also further drying is a pressed powder.
The object of the present invention is to provide a kind of preparation method of Bitter Melon P.E, extract the balsam pear extract that obtains by this method, is Main Ingredients and Appearance with momordica saponins, bitter melon polysaccharide and bitter gourd polypeptide.
Preparation method of the present invention comprises following process successively:
(1) balsam pear powder is got in the full dried fruit of fresh balsam pear is dry, pulverizing, and the water that adds at least 4 times of amounts of balsam pear powder weight stirs into slurries, it is 4.0~5.5 that slurries drip acid to pH value, be heated to 75 ℃~100 ℃ extractions at least 2 hours, extraction is cooled to room temperature after finishing, and is standby;
(2) the extract temperature remains on 45 ℃~55 ℃, transfers pH to 3.0~4.0 with acid, adds cellulase and pectase, after stirring evenly, keeps enzymolysis under the condition of pH to 3.0~4.0,45 ℃~55 ℃;
(3) enzymolysis is heated to 95 ℃~100 ℃ after finishing, and be that 5min~20min carries out enzyme-deactivating heat time heating time, is cooled to room temperature, and ultrasonic wave is handled;
(4) be heated to 75 ℃~100 ℃ after the ultrasonic wave processing and extract more than 2 hours, extraction is cooled to room temperature after finishing, and is centrifugal, filters, and gets filtrate 1.;
(5) filter residue adds the above water of 2 times of amounts again, is heated to 75 ℃~100 ℃ and extracts more than 2 hours, and extraction is cooled to room temperature after finishing, pH to 3.0~4.0 are transferred in acid, add cellulase and pectase and carry out enzymolysis, keep pH to 3.0~4.0, enzymolysis under 45 ℃~55 ℃ the condition, enzymolysis is heated to 95 ℃~100 ℃ after finishing, and be that 5min~20min carries out enzyme-deactivating heat time heating time, is cooled to room temperature, ultrasonic wave is handled, centrifugal, filter, get filtrate 2..
(6) merging filtrate 1. with filtrate 2., cross hollow-fibre membrane, get through liquid and be evaporated to 10%~40% of original volume, centrifugal, filter, collect filtrate, promptly get balsam pear extract.
Can also further carry out the dry pressed powder extract that gets.
The part by weight of cellulase and pectase is generally 1~3:1~3, adjusts according to the content of cellulose and pectin in the balsam pear kind.
The method that cellulase that the present invention adopts and pectase carry out enzymolysis, and the method that adopts ultrasonic processing, can make momordica saponins, bitter melon polysaccharide and bitter gourd polypeptide etc. nutritional labeling is separated as much as possible to extract, reach and improve the purpose that yield and nutritional labeling are fully extracted.
The acid that described adjust pH is used is the acid of phosphoric acid, citric acid, hydrochloric acid, sulfuric acid or phosphoric acid, citric acid, hydrochloric acid and sulfuric acid combination.
The present invention grasps the enzymolysis process by the control enzymolysis time, enzymolysis time is short, and the insufficient yield that causes of enzymolysis is low, but the enzymolysis time process can extend manufacture cycle again, increase cost and increase contaminated chance, the enzymolysis time in general step (2) and the step (5) is 60~180 minutes.
Ultrasonic wave treatment conditions in general step (3) and the step (5) are 500W~5000W, 20 min~90min.The method of Separation of Solid and Liquid is that first suction filtration gets supernatant in general step (4) and the step (5), and residue is centrifugal with centrifuge, collects supernatant, merges the supernatant of suction filtration and centrifugal gained, carries out suction filtration again, isolates filtrate.
The molecular cut off 100,000 of hollow-fibre membrane in the general step (6).The condition that decompression concentrates in the general step (6) is 40 ℃~80 ℃, 0.04~0.10Mpa.
The present invention is directed to characteristics to the balsam pear effective component, plain enzyme is tieed up in employing and pectase carries out enzymolysis to it, and the method that adopts ultrasonic processing, purpose is as much as possible nutritional labelings such as momordica saponins, bitter melon polysaccharide and bitter gourd polypeptide to be extracted, this method has following advantage:
1, the direct water of the present invention contains the balsam pear extract of nutritional labelings such as momordica saponins, bitter melon polysaccharide and bitter gourd polypeptide as solvent extraction, do not use organic solvent in process, product safety is pollution-free, leaching process is free from environmental pollution, extracting method is simple fast, extraction cost is low.
2, the present invention adopts cellulase and pectase to take the enzymolysis balsam pear, and the method that adopts ultrasonic processing, reach the recovery rate that improves the balsam pear nutrient material, obtain nutritious extract, wherein contain nutritional labelings such as momordica saponins, bitter melon polysaccharide and bitter gourd polypeptide.
3, the present invention adopts the fruit (comprising the balsam pear seed) of whole balsam pear to extract balsam pear extract, reached the purpose of extracting bitter gourd polypeptide, make the nutritional labeling of balsam pear extract more abundant, moreover be exactly to have reached rationally to utilize raw-material purpose, avoid waste and cause environmental pollution.
4, the present invention adopts the method that concentrates balsam pear extract, makes balsam pear extract nutritional labeling concentration higher, and effect is more obvious.
The specific embodiment
Below by specific embodiment the present invention is described in further detail.
Get the 1.0kg balsam pear powder, the water that adds 8.0L stirs into slurries, and slurries drip 4.5%(w%) hydrochloric acid to pH value is 4.2 ± 0.2, is heated to 85 ℃ ± 5 ℃ and extracts 3 hours, is cooled to room temperature after extracting end.Slurries 4.5%(w%) hydrochloric acid is transferred pH to 3.5 ± 0.2, add the ratio that 4 million international units (than vigor) cellulase and every kg balsam pear powder add 4 million international units (than vigor) pectase in every kg balsam pear powder and add the cellulase of 4 million international units (than vigor) and the pectase of 4 million international units (than vigor), after stirring evenly, keep enzymolysis 90min under the condition of pH to 3.5 ± 0.2,48 ℃ ± 2 ℃.Enzymolysis is heated to 100 ℃ after finishing, and be that 10min carries out enzyme-deactivating heat time heating time, is cooled to room temperature, handles 50min with 500W~5000W ultrasonic wave.Be heated to 85 ℃ ± 5 ℃ after the ultrasonic wave processing and extracted 2 hours, extraction is cooled to room temperature after finishing, and is centrifugal, filters, and gets 1. 6.0L of filtrate;
Filter residue adds the water of 6.0L again, being heated to 85 ℃ ± 5 ℃ extracted 2 hours, be cooled to room temperature after extracting end, use 4.5%(w%) hydrochloric acid accent pH to 3.5 ± 0.2, add in every kg balsam pear powder that pectase that ratio that 4 million international units (than vigor) cellulase and every kg balsam pear powder add 4 million international units (than vigor) pectase adds the cellulase of 4 million international units (than vigor) and 4 million international units (than vigor) adds cellulase and pectase carries out enzymolysis, keep pH to 3.5 ± 0.2, enzymolysis under 48 ℃ ± 2 ℃ the condition, enzymolysis is heated to 100 ℃ after finishing, be that 10min carries out enzyme-deactivating heat time heating time, be cooled to room temperature, ultrasonic wave is handled 50min, centrifugal, filter, get 2. 4.0L of filtrate.
2. 1. merging filtrate get filtrate 10.0L with filtrate, and molecular cut off is 100,000 hollow-fibre membrane excessively, must see through liquid 9.5L, get through liquid in the vacuum concentrating instrument, and 58 ℃, 0.05Mpa.Be evaporated to volume and be 2.2L, centrifugal, filter, collect filtrate and get 2.0L, both be balsam pear extract.Detections such as momordica saponins detection, polysaccharide detection, polypeptide detection and acidity are carried out in sampling, and are standby.
Assay:
1. momordica saponins content: 9.73 mg/ml(vanillic aldehydes, one perchloric acid colorimetric method).
2. polysaccharide is differentiated: phenol-sulphate reagent reaction (positive).
3. polyoses content: 13.41mg/ml(measures with phenol-sulfuric acid process).
4. polypeptide is differentiated: get this product 1ml, add ninhydrin solution number droplet, heating shows purple, illustrates that this product is for containing polypeptide solution.
5. content of peptides: 6.25mg/ml(forint phenol method).
The pH value is 2.92 (two appendix VI of Chinese Pharmacopoeia version in 2005 H).

Claims (7)

1. the preparation method of a Bitter Melon P.E may further comprise the steps successively:
(1) balsam pear powder is got in the full dried fruit of fresh balsam pear is dry, pulverizing, and the water that adds at least 4 times of amounts of balsam pear powder weight stirs into slurries, it is 4.0~5.5 that slurries drip acid to pH value, be heated to 75 ℃~100 ℃ extractions at least 2 hours, extraction is cooled to room temperature after finishing, and is standby;
(2) the extract temperature remains on 45 ℃~55 ℃, transfers pH to 3.0~4.0 with acid, adds cellulase and pectase, after stirring evenly, keeps enzymolysis under the condition of pH to 3.0~4.0,45 ℃~55 ℃;
(3) enzymolysis is heated to 95 ℃~100 ℃ after finishing, and be that 5min~20min carries out enzyme-deactivating heat time heating time, is cooled to room temperature, and ultrasonic wave is handled;
(4) be heated to 75 ℃~100 ℃ after the ultrasonic wave processing and extract more than 2 hours, extraction is cooled to room temperature after finishing, and is centrifugal, filters, and gets filtrate 1.;
(5) add the water that is at least 2 times of amount filter residue weight in the filter residue again, be heated to 75 ℃~100 ℃ extractions at least 2 hours, extraction is cooled to room temperature after finishing, pH to 3.0~4.0 are transferred in acid, add cellulase and pectase and carry out enzymolysis, keep pH to 3.0~4.0, enzymolysis under 45 ℃~55 ℃ the condition, enzymolysis is heated to 95 ℃~100 ℃ after finishing, and be that 5min~20min carries out enzyme-deactivating heat time heating time, is cooled to room temperature, ultrasonic wave is handled, centrifugal, filter, get filtrate 2.;
(6) merging filtrate 1. with filtrate 2., cross hollow-fibre membrane, get through liquid and be evaporated to 10%~40% of original volume, centrifugal, filter, collect filtrate, balsam pear extract.
2. the preparation method of Bitter Melon P.E according to claim 1 is characterized in that: the acid that described adjust pH is used is the acid of phosphoric acid, citric acid, hydrochloric acid, sulfuric acid or phosphoric acid, citric acid, hydrochloric acid and sulfuric acid combination.
3. the preparation method of Bitter Melon P.E according to claim 1, it is characterized in that: the enzymolysis time in described step (2) and the step (5) is 60~180 minutes.
4. the preparation method of Bitter Melon P.E according to claim 1, it is characterized in that: the ultrasonic wave treatment conditions in described step (3) and the step (5) are 500W~5000W, 20 min~90min.
5. the preparation method of Bitter Melon P.E according to claim 1 is characterized in that: the molecular cut off 100,000 of hollow-fibre membrane in the described step (6).
6. the preparation method of Bitter Melon P.E according to claim 1 is characterized in that: the condition that decompression concentrates in the described step (6) is 40 ℃~80 ℃, 0.04~0.10Mpa.
7. the preparation method of Bitter Melon P.E according to claim 1 is characterized in that: the weight ratio of cellulase and pectase is 1~3:1~3 in described step (2) and the step (5).
CN2009100009359A 2009-01-22 2009-01-22 Bitter melon extract preparation method Active CN101461514B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2009100009359A CN101461514B (en) 2009-01-22 2009-01-22 Bitter melon extract preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2009100009359A CN101461514B (en) 2009-01-22 2009-01-22 Bitter melon extract preparation method

Publications (2)

Publication Number Publication Date
CN101461514A CN101461514A (en) 2009-06-24
CN101461514B true CN101461514B (en) 2011-11-30

Family

ID=40802463

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009100009359A Active CN101461514B (en) 2009-01-22 2009-01-22 Bitter melon extract preparation method

Country Status (1)

Country Link
CN (1) CN101461514B (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102373218B (en) * 2010-08-13 2014-06-04 浙江日升昌药业有限公司 Nucleic acid sequence coding Momordica charantia polypeptide P
WO2012101657A1 (en) 2011-01-28 2012-08-02 Piramal Life Sciences Ltd. Process for preparation of a herbal extract
CN102232590B (en) * 2011-08-09 2013-03-13 韩金光 Mature vinegar and bitter gourd juice beverage and preparation method thereof
CN102246986B (en) * 2011-08-09 2013-01-23 韩金光 Bitter melon juice drink and preparation method thereof
CN102675157A (en) * 2012-04-10 2012-09-19 福州大学 Bitter gourd Maillard reaction product with blood sugar regulating function and preparation method thereof
CN103211145B (en) * 2013-04-01 2015-01-21 沈阳农业大学 Preparation method of active polysaccharide peptide with protective effect on alcoholic liver injury and compound of active polysaccharide peptide
CN105820262A (en) * 2016-03-31 2016-08-03 唐云龙 Momordica polysaccharide extracting method
CN106263024A (en) * 2016-08-12 2017-01-04 福建中烟工业有限责任公司 A kind of reduce the method for phenol content in cigarette smoke
CN106422403A (en) * 2016-09-27 2017-02-22 桂林茗兴生物科技有限公司 Preparation method of Baccaurea ramiflora extract
CN106465930A (en) * 2016-09-27 2017-03-01 桂林茗兴生物科技有限公司 A kind of extraction process of Lonicera standishii soluble sugar
CN106589149B (en) * 2016-11-12 2020-04-14 徐州医科大学 Extraction method of momordica polysaccharide, product and application thereof
CN108851076A (en) * 2018-06-06 2018-11-23 李云逸 A kind of preparation method and application of Bitter Melon P.E
TWI684412B (en) * 2018-08-13 2020-02-11 李國龍 Manufacturing method of mountain bitter gourd extract
CN109123543A (en) * 2018-08-20 2019-01-04 安徽蜂献蜂业有限公司 A kind of queen bee paste composition with anti-fatigue effect of refreshing oneself
CN110801024A (en) * 2019-10-25 2020-02-18 运鸿集团股份有限公司 Polysaccharide composite polypeptide for reducing blood sugar, blood fat and glycosylated hemoglobin and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1211441A (en) * 1998-04-22 1999-03-24 徐光明 Preparation method for medicine of reducing blood sugar using natural bitter gourd extract
CN1456337A (en) * 2003-05-30 2003-11-19 广西壮族自治区中国科学院广西植物研究所 Balsam pear products containing multiple hypoglycemic active composition and preparation thereof
JP2004250363A (en) * 2003-02-19 2004-09-09 Meiji Milk Prod Co Ltd alpha-GLUCOSIDASE INHIBITOR
CN1872135A (en) * 2006-04-21 2006-12-06 江苏大学 Composition of bleeding liquor of bitter melon, preparation method and usage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1211441A (en) * 1998-04-22 1999-03-24 徐光明 Preparation method for medicine of reducing blood sugar using natural bitter gourd extract
JP2004250363A (en) * 2003-02-19 2004-09-09 Meiji Milk Prod Co Ltd alpha-GLUCOSIDASE INHIBITOR
CN1456337A (en) * 2003-05-30 2003-11-19 广西壮族自治区中国科学院广西植物研究所 Balsam pear products containing multiple hypoglycemic active composition and preparation thereof
CN1872135A (en) * 2006-04-21 2006-12-06 江苏大学 Composition of bleeding liquor of bitter melon, preparation method and usage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
高晓明,张效林,李振武.苦瓜皂甙的复合酶法提取与大孔数值纯化工艺研究.《化学与生物工程》.2006,第23卷(第3期),51-53. *

Also Published As

Publication number Publication date
CN101461514A (en) 2009-06-24

Similar Documents

Publication Publication Date Title
CN101461514B (en) Bitter melon extract preparation method
CN101508690B (en) Novel methods for simultaneously extracting tea polyphenol, tea polysaccharide and caffeinum from tea
CN102417546B (en) Extraction method of rose crude polysaccharide
CN102786604A (en) Method for one-time separation and extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum
CN100548150C (en) The preparation method of coconut extractive
CN102429184B (en) Instant asparagus powder and preparation method thereof
CN105030878A (en) Method for extracting multiple active ingredients of blueberry fruits
CN104788511A (en) Method for preparing persimmon tannins with high micromolecular tannin content from persimmons
CN105693781A (en) Technology for extracting tea polyphenol in tea leaves
CN103735633B (en) A kind of preparation method of instant gardenia powder
CN102234671A (en) Method for extracting polysaccharide from longan pulp
CN108484785A (en) A kind of method and monosaccharide component identification method of the extraction purification polysaccharide from gumbo
CN1810763B (en) Honeysuckle chlorogenic acid extracting technology
CN106726889A (en) The extracting method of Camellia nitidissima active ingredient and prepare the purposes of health products
CN105037572B (en) A kind of method that burdock polysaccharide is extracted from radix bardanae
CN101709094A (en) Method for separating sweet tea polysaccharide by ultrafiltration membranes
CN1304488C (en) Technique for extracting edible natural red color element from psammophyte of nitraria
CN101659793A (en) Method for extracting gynura bicolor purpurin
CN102198049A (en) Traditional Chinese medicine preparation method and device
CN107573438A (en) A kind of method that polysaccharide is extracted in the pericarp from passion fruit
CN1176748A (en) Art for prodn. polyose and poly phenols mixed crystal extracted from plain tea leaves and old leaves
CN100419031C (en) Production of beet red coloring matter jointed phytolaccic saponin from phytolaccic berry
CN106518653A (en) Membrane extraction process for hydroxycitric acid
CN103936874A (en) Method for extracting sea cucumber polysaccharide from sea cucumber boiled juice
CN101869266A (en) Multi-trace elements-enriched dioscorease rhizome extract and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
LICC Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20090624

Assignee: Hainan Stand Bio-Technology Co., Ltd.

Assignor: Han Jinguang

Contract record no.: 2014990000181

Denomination of invention: Preparation method and application of bitter-gourd extract

Granted publication date: 20111130

License type: Exclusive License

Record date: 20140404

COR Change of bibliographic data

Free format text: CORRECT: ADDRESS; FROM: 300201 HEXI, TIANJIN TO: 570311 HAIKOU, HAINAN PROVINCE

ASS Succession or assignment of patent right

Owner name: HAINAN STANDARD BIO-TECHNIQUE CO., LTD.

Free format text: FORMER OWNER: HAN JINGUANG

Effective date: 20150129

C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20150129

Address after: Trough of Haikou national hi tech Industrial Development Zone, two cross road 570311 Hainan city of Haikou province No. 10

Patentee after: Hainan standard biological Polytron Technologies Inc

Address before: 300201, room 2201, North finance building, 5 Youyi Road, Tianjin, Hexi District

Patentee before: Han Jinguang

PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: Preparation method and application of bitter-gourd extract

Effective date of registration: 20170307

Granted publication date: 20111130

Pledgee: Wenchang Hainan rural commercial bank Limited by Share Ltd

Pledgor: Hainan standard biological Polytron Technologies Inc

Registration number: 2017460000003

PE01 Entry into force of the registration of the contract for pledge of patent right