CN1861636A - Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose - Google Patents
Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose Download PDFInfo
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- CN1861636A CN1861636A CN 200510025711 CN200510025711A CN1861636A CN 1861636 A CN1861636 A CN 1861636A CN 200510025711 CN200510025711 CN 200510025711 CN 200510025711 A CN200510025711 A CN 200510025711A CN 1861636 A CN1861636 A CN 1861636A
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Abstract
A process for preparing the crude polyose from hedgehog fungus by semi-bionic extracting method features that the semi-bionic extraction instead of water extraction and alcohol deposition is used, and the flocculating deposition is used to remove viscose impurities and residual heavy metal, resulting in higher output rate.
Description
Technical field:
Belonging to biomedicine field, exactly is the extraction processing technology at medicinal fungi effective constituent polysaccharide.
Background technology:
Hericium erinaceus (Bull. Ex Fr.) Pers. has examines expensive food, pharmaceutical use, and multiple digestive tract diseases is all had better curative effect, can improve the human immunological competence.Chinese scholars shows that through years of researches it is the Hericium erinaceus (Bull. Ex Fr.) Pers. polysaccharide that bioactive chemical ingredients is arranged in the Hericium erinaceus (Bull. Ex Fr.) Pers. most.Along with going deep into that the Hericium erinaceus (Bull. Ex Fr.) Pers. pharmaceutical use is studied, the biological function of Hericium erinaceus (Bull. Ex Fr.) Pers. polysaccharide causes numerous scholars and developer's concern.The product that with the Hericium erinaceus (Bull. Ex Fr.) Pers. is main raw material is existing a lot, and these products all are to utilize the thing of carrying entirely of the mycelium of Hericium erinaceus (Bull. Ex Fr.) Pers. or sporophore to fill a prescription and make, but the preparation technology of these products relatively tradition is simple, wherein the content of polysaccharide is not very high.Because extraction process is simple, yield is not high, and content is lower, causes the production cost height, and efficacy effect is not remarkable.
The original extracting method of hedgehogt fungus crude polysaccharose is mainly taken over the traditional preparation process method of fungus polysaccharide, after hot water extraction, directly united extraction liquid concentrates, and uses alcohol precipitation again, after will precipitating the part centrifugation and going out, obtain the Crude polysaccharides goods by spraying drying after vacuum-drying or the water dissolution.Can only propose wherein water-soluble polysaccharide with single water extraction, also have the polysaccharide in a lot of cell walls to be difficult to extract.To further utilize Hericium erinaceus (Bull. Ex Fr.) Pers., prepare its deep processed product, have only and improve extraction process on the original basis, could prepare the hedgehogt fungus crude polysaccharose extract of high density.The deficiency that traditional water is carried alcohol precipitating method mainly contains: 1) the Crude polysaccharides yield of Ti Quing is low, and is not high to utilization ratio of raw materials; 2) many pectin substance class viscous substances are arranged in the Hericium erinaceus (Bull. Ex Fr.) Pers., increased the viscosity of extracting solution during extraction, be difficult for drying, loss was bigger when spray was done; 3) polysaccharide content is not high in the extract that obtains, and influences the performance of its efficacy effect.
Summary of the invention:
The present invention will provide a kind of extraction yield height, a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. polysaccharide process for half bionic extracting preparing that polysaccharide content is also high.Core technology scheme of the present invention is: substitute simple water extraction with semi-bionic extraction, remove heavy metal residual in sticky contaminant and the water with flocculation sediment.
Concrete scheme is: Hericium erinaceus (Bull. Ex Fr.) Pers. with the extraction with aqueous solution of different pH three times, merged the concentrating under reduced pressure extracting solution and transfers pH to make flocculation agent to neutral back with chitosan, precipitate the heavy metal in non-polyose impurity of viscosity and the water source,
The centrifugal post precipitation of removing adds alcohol precipitation polysaccharide macromolecular functional component again.To precipitate part and fling to alcohol final vacuum drying, promptly obtain the hedgehogt fungus crude polysaccharose extract.
Extract yield and polysaccharide content behind enforcement the present invention and all more than 10%, see that following index is relatively than former method raising:
Can see that from last table state's exterior-applied liquid medicine carries alcohol deposition method extraction Hericium erinaceus (Bull. Ex Fr.) Pers. series products and the existing single water extraction extraction yield of China is similar, polysaccharide content then exceeds nearly one times.After using present technique, extract yield and not only improve more than 10%, and polysaccharide content exceeds nearly ten percentage points than external technology.Hedgehogt fungus crude polysaccharose extracts the raising of yield to the most direct influence that reduced production costs, and by this implementation of processes, the preparation cost of hedgehogt fungus crude polysaccharose is saved greatly.
Description of drawings:
Fig. 1 process flow diagram of the present invention
Embodiment: existing combined process schematic flow sheet describes technological process in detail
1) the raw material decon cleans
Hericium erinaceus 1 raw material is removed impurity 2, and it is granular to carry out 3 one-tenth little of coarse reductions with the raw material Roughpulverizer, is dosed in the extractor.
2) water treatment
Drinking public water supply 4 carries out water treatment 5 by water treating device, removes the nuisance in the water sources such as heavy metal, organic matter.
3) weakly acidic water extracts
Regulate to extract water between the pH5-5.5 6 with weak acid, add in the extractor, add volume of water for dropping into 10 times of material.Heating, also controlled temperature is at 50-60 ℃, and atmospheric pressure state extracted 1 hour, and with sheet frame press filtration 7, filtrate 8 is put into storage tank.Residue 9 is stayed to do in the jar to extract for the second time and is used.
4) alkalescent water extracts
The treating water that adds 10 times of volume of material in the extractor is regulated with alkali and to be extracted water between the pH7.5-8 10.Heating, also controlled temperature is at 50-60 ℃, and atmospheric pressure state extracted 2 hours, sheet frame press filtration 11, and filtrate 12 is put into storage tank.Residue 13 is stayed to do in the jar to extract for the third time and is used.
5) neutral water extracts
The water that adds 10 times of volume of material in the extractor is with acid for adjusting pH neutrality 14.Heating, also controlled temperature is at 95-100 ℃, and atmospheric pressure state extracted 1 hour, filter press, and filtrate 15 is put into storage tank.Residue is emitted and is discarded.
6) united extraction liquid is transferred pH neutrality
Merge No. three times extracting solution, transfer to neutral 16 with acid or alkali again according to recording pH.
7) concentrating under reduced pressure
Extracting solution is pumped in the concentrating under reduced pressure jar, carry out concentrating under reduced pressure 17,65～70 ℃ of concentration tank temperature, pressure is less than 0.1MPa.Be concentrated to proportion 1.050～1.080 (50 ℃ of heat are surveyed).
8) flocculation sediment
Select for use chitosan as flocculating settling agent, with heavy metal chelating sedimentation 18 residual in viscous substance in the extracting solution and the water quality.First acetic acid with 2% is made into 1% solution, add-on 10% when adding chitosan.
7) centrifugal removal sediment 19
4000rmp centrifugation, disgorging, supernatant liquor are further done alcohol precipitation and are used.
8) alcohol precipitation 20
Slowly add 95% ethanol that is equivalent to 4 times of liquor capacities in clear liquor, mixing spends the night.
9) centrifugal collecting precipitation 21
4000rmp centrifugation supernatant liquor, the collecting precipitation thing.Supernatant liquor reclaims ethanol 22 to distillate ethanol degree＜10%23.
10) precipitation vacuum-drying 24
With the throw out high plate of packing into equably, material is high be no more than dish high 1/3.Placed 60～65 ℃ of baking oven inner dryings earlier 4 hours.Vacuumize drying again, 65 ℃ of temperature controls, negative pressure 0.1MPa, vacuum-drying 8 hours.
11) crushing packing
Take out dry thing, the pulverizer that is cooled with circulating water is pulverized material, crosses 80 mesh sieves.Promptly obtain hedgehogt fungus crude polysaccharose 25 extracts, measure packing in accordance with regulations with thin aluminum bag.
Claims (10)
1, process for half bionic extracting preparing hedgehogt fungus crude polysaccharose comprises the raw material decon and cleans, and water treatment is characterized in that: use semi-bionic extraction, remove heavy metal residual in sticky contaminant and the water with flocculation sediment;
Concrete steps are: with Hericium erinaceus (Bull. Ex Fr.) Pers. with the extraction with aqueous solution of different pH three times, extract with weakly acidic water respectively, alkalescent water extracts, neutral water extracts: united extraction liquid, concentrating under reduced pressure, and transfer pH to make flocculation agent with chitosan the extracting solution to neutral back, and the heavy metal in precipitation non-polyose impurity of viscosity and the water source, the centrifugal post precipitation of removing adds alcohol precipitation polysaccharide macromolecular functional component again; Part be will precipitate and alcohol final vacuum drying, crushing packing flung to.
2, preparation method according to claim 1 is characterized in that: described is in the extractor that adds material with the weakly acidic water extraction, adds volume of water for water between the pH5-5.5 that drops into 10 times of materials, heats at 50-60 ℃ atmospheric pressure state extraction 1 hour.
3, preparation method according to claim 1 is characterized in that: described alkalescent water extracts, and is the treating water that adds 10 times of volume of material in extractor, regulates with alkali and extracts water between the pH7.5-8, and the 50-60 ℃ of atmospheric pressure state of heating extracted 2 hours.
4, preparation method according to claim 1 is characterized in that: described neutral water extracts, and is the water that adds 10 times of volume of material in extractor, with acid for adjusting pH 7, heat and controlled temperature at 95-100 ℃, atmospheric pressure state extraction 1 hour.
5, preparation method according to claim 1, it is characterized in that: described concentrating under reduced pressure is that extracting solution is pumped in the concentrating under reduced pressure jar, carries out concentrating under reduced pressure, 65～70 ℃ of concentration tank temperature, pressure is less than 0.1Mpa, is concentrated to 50 ℃ of proportions 1.050～1.080 when surveying.
6, preparation method according to claim 1, it is characterized in that: described flocculation sediment, be to select for use chitosan as flocculating settling agent, with heavy metal chelating sedimentation residual in viscous substance in the extracting solution and the water quality, first acetic acid with 2% is made into 1% solution, add-on 10% when adding chitosan.
7, preparation method according to claim 1 is characterized in that: the described centrifugal sediment of removing is to use 4000rmp centrifugation, disgorging.
8, preparation method according to claim 1 is characterized in that: described alcohol precipitation is slowly to add 95% ethanol that is equivalent to 4 times of liquor capacities in clear liquor, and mixing spends the night.
9, preparation method according to claim 1 is characterized in that: described vacuum-drying is with the throw out high plate of packing into equably, material is high be no more than dish high 1/3; Placed 60～65 ℃ of baking oven inner dryings earlier 4 hours; Vacuumize drying again, 65 ℃ of temperature controls, negative pressure 0.1MPa, vacuum-drying 8 hours.
10, preparation method according to claim 1 is characterized in that: described crushing packing is the dry thing that will take out, and the pulverizer that is cooled with circulating water is pulverized, and crosses 80 mesh sieves, promptly obtains the hedgehogt fungus crude polysaccharose extract, measures packing in accordance with regulations with thin aluminum bag.
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| CNB2005100257115A CN100398564C (en) | 2005-05-10 | 2005-05-10 | Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose |
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| CNB2005100257115A CN100398564C (en) | 2005-05-10 | 2005-05-10 | Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose |
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Cited By (12)
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| CN102344470A (en) * | 2011-07-29 | 2012-02-08 | 余先纯 | Method for extracting wild persimmon leaf tannin by ultrasonic wave auxiliary semi-bionic method |
| CN101536740B (en) * | 2009-04-09 | 2012-06-20 | 徐财泉 | Method of removing heavy metal in edible fungi polysaccharide extraction liquid |
| CN102786606A (en) * | 2012-09-05 | 2012-11-21 | 龙海市颖欣农业科技有限公司 | Processing method of lentinan |
| CN103766914A (en) * | 2014-01-24 | 2014-05-07 | 侯梦斌 | Semi-bionic extraction method of dietary supplement |
| CN104059160A (en) * | 2014-06-25 | 2014-09-24 | 上海市农业科学院 | Hericium erinaceus cell wall polysaccharide and preparation method thereof |
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN104403020A (en) * | 2014-11-28 | 2015-03-11 | 哈尔滨墨医生物技术有限公司 | Semi-bionic lentinan extraction method |
| CN104497159A (en) * | 2014-12-31 | 2015-04-08 | 华东理工大学 | Method for purifying edible fungus polysaccharide by using enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology |
| CN105581336A (en) * | 2015-12-05 | 2016-05-18 | 戴群 | Original ecological active essence of fresh kudzuvine root as well as preparation method and preparation thereof |
| CN105693880A (en) * | 2016-04-28 | 2016-06-22 | 广东太阳神集团有限公司 | Protein removal method for hericium erinaceus polysaccharide extracting solution |
| CN106883304A (en) * | 2017-03-06 | 2017-06-23 | 南昌大学 | Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus |
| CN111484567A (en) * | 2020-06-01 | 2020-08-04 | 枣庄学院 | Extraction and preparation method of polysaccharide in long red dates, polysaccharide and application |
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| CN101966224B (en) * | 2010-10-20 | 2012-01-04 | 无锡正大畜禽有限公司 | Method for producing astragalus and codonopsis pilosula compound extract |
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| CN1071170A (en) * | 1991-09-25 | 1993-04-21 | 梁芳亭 | Acidic technique for production of proteoglycan with hedgehog hydnum mycelium |
| CN1300175C (en) * | 2003-05-21 | 2007-02-14 | 吉林省生物研究所 | Medicinal polysaccharide component of spinulate hedgehog fungus, its prepn and medicinal composition |
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2005
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| CN101536740B (en) * | 2009-04-09 | 2012-06-20 | 徐财泉 | Method of removing heavy metal in edible fungi polysaccharide extraction liquid |
| CN102344470A (en) * | 2011-07-29 | 2012-02-08 | 余先纯 | Method for extracting wild persimmon leaf tannin by ultrasonic wave auxiliary semi-bionic method |
| CN102786606B (en) * | 2012-09-05 | 2015-05-20 | 龙海市颖欣农业科技有限公司 | Processing method of lentinan |
| CN102786606A (en) * | 2012-09-05 | 2012-11-21 | 龙海市颖欣农业科技有限公司 | Processing method of lentinan |
| CN103766914B (en) * | 2014-01-24 | 2016-02-17 | 侯梦斌 | A kind of semi-bionic extraction method method of dietary supplements |
| CN103766914A (en) * | 2014-01-24 | 2014-05-07 | 侯梦斌 | Semi-bionic extraction method of dietary supplement |
| CN104059160A (en) * | 2014-06-25 | 2014-09-24 | 上海市农业科学院 | Hericium erinaceus cell wall polysaccharide and preparation method thereof |
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN104403020A (en) * | 2014-11-28 | 2015-03-11 | 哈尔滨墨医生物技术有限公司 | Semi-bionic lentinan extraction method |
| CN104497159A (en) * | 2014-12-31 | 2015-04-08 | 华东理工大学 | Method for purifying edible fungus polysaccharide by using enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology |
| CN105581336A (en) * | 2015-12-05 | 2016-05-18 | 戴群 | Original ecological active essence of fresh kudzuvine root as well as preparation method and preparation thereof |
| CN105581336B (en) * | 2015-12-05 | 2020-02-07 | 戴群 | Fresh radix puerariae original ecological active essence and preparation method and preparation thereof |
| CN105693880A (en) * | 2016-04-28 | 2016-06-22 | 广东太阳神集团有限公司 | Protein removal method for hericium erinaceus polysaccharide extracting solution |
| CN106883304A (en) * | 2017-03-06 | 2017-06-23 | 南昌大学 | Heterogeneity polysaccharide is comprehensively prepared and purification process in a kind of Hericium erinaceus |
| CN111484567A (en) * | 2020-06-01 | 2020-08-04 | 枣庄学院 | Extraction and preparation method of polysaccharide in long red dates, polysaccharide and application |
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| CN100398564C (en) | 2008-07-02 |
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Effective date of registration: 20100608 Address after: 221423 No. 35 Nanhua Road, Shanghai, Minhang District Co-patentee after: Shanghai Baixin Bio-tech Co.,Ltd. Patentee after: Shanghai Academy of Agricultural Sciences Address before: 221423 No. 35 Nanhua Road, Shanghai, Minhang District Patentee before: Shanghai Academy of Agricultural Sciences |
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