CN105693880A - Protein removal method for hericium erinaceus polysaccharide extracting solution - Google Patents
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Abstract
The invention relates to the technical field of protein removal for hericium erinaceus polysaccharide extracting solutions, in particular to a protein removal method for a hericium erinaceus polysaccharide extracting solution.The method comprises the steps that 1, a chitosan-acetic acid solution is prepared; 2, the hericium erinaceus polysaccharide extracting solution is mixed with the chitosan-acetic acid solution; 3, centrifugal treatment is carried out for the first time; 4, centrifugal treatment is carried out for the second time.The protein removal method for the hericium erinaceus polysaccharide extracting solution has the advantages of being safe, nontoxic, free of pollution, environmentally friendly, high in protein removal rate and low in polysaccharide losing rate, glycosidic bond breakage can be avoided, and then the polysaccharide structure and activity can be ensured.In addition, the protein removal method for the hericium erinaceus polysaccharide extracting solution has the advantages of being simple in treatment, easy to operate, low in production cost and applicable to large-scale production, and can be applied to production of relevant food health care products.
Description
Technical field
The present invention relates to the Deproteinated technical field of polysaccharide extraction liquid, particularly relate to the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution。
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. (Hericiumerinaceus) is precious medicated diet dual-purpose bacterium。Modern study shows that Hericium erinaceus (Bull. Ex Fr.) Pers. contains various active composition, such as polysaccharide, terpenoid, phenolic compound etc.。Wherein, it has already been proven that what have functional activity in Hericium erinaceus (Bull. Ex Fr.) Pers. is mainly composed of Hericium Erinaceus Polysaccharide。Research about Hericium Erinaceus Polysaccharide is relatively many, and research shows that it has immunomodulating, antitumor, protects the physiologically actives such as stomach, and the medicine that Hericium Erinaceus Polysaccharide is made and health product are also loved by the people, and DEVELOPMENT PROSPECT is wide。But mostly Hericium erinaceus (Bull. Ex Fr.) Pers. relevant healthcare product are that Hericium erinaceus (Bull. Ex Fr.) Pers. extracting solution is made, and the functional activity of Hericium Erinaceus Polysaccharide is relevant with its moderate purity, by impurity effects such as protein therein。Hericium erinaceus (Bull. Ex Fr.) Pers. extracting solution is the mixture of the compositions such as polysaccharide, protein, cellulose, and active effect is affected, and is also unfavorable for the raising of product quality。Therefore, improve the polysaccharide concentration of Hericium erinaceus (Bull. Ex Fr.) Pers. goods, reduce the concentration of other compositions, the active effect improving Hericium erinaceus (Bull. Ex Fr.) Pers. correlated product is had great importance。In the composition of Hericium erinaceus (Bull. Ex Fr.) Pers. goods, except polysaccharide, what content was higher is protein, and therefore, it is the important step improving polysaccharide concentration that Hericium erinaceus (Bull. Ex Fr.) Pers. extracting solution carries out deproteinization。
Hericium erinaceus (Bull. Ex Fr.) Pers. extracting solution method for removing protein conventional in currently available technology is Sevage method, also has trichloroacetic acid method, trifluorotrichloroethane method, hydrochloric acid method, sodium-hydroxide method etc. in the method for removing protein of fungus polysaccharide extracting solution。Hydrochloric acid method, sodium-hydroxide method are owing to using soda acid, it is easy to make polysaccharide be hydrolyzed, and glycosides bond fission affects structure and the activity of polysaccharide。Sevage method, trichloroacetic acid method, trifluorotrichloroethane method etc. are owing to all using organic solvent, easily cause the residual of organic solvent, and the chloroform used in Sevage method, n-butyl alcohol, the trichloroacetic acid that trichloroacetic acid method is used is toxic chemical, it is unfavorable for the health of human body, there is potential safety hazard, be not therefore suitable for the exploitation of food product。In order to keep structure and the activity of Hericium Erinaceus Polysaccharide, and reducing using and remaining of organic reagent, the deproteinization technical method of exploitation safety non-toxic, environmental protection and mild condition just has important application value and real value。
Summary of the invention
Present invention aims to weak point of the prior art and the method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of a kind of safety non-toxic, environmental protection is provided。
For reaching above-mentioned purpose, the present invention is achieved through the following technical solutions。
Thering is provided the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution, it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution is mixed homogeneously, obtains the first mixture；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is under uniform temperature standing certain time；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtain the second supernatant, then the second supernatant is under uniform temperature placement certain time, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization。
In technique scheme, described step one is prepared in chitosan-acetum step, and in described acetum, the concentration of volume percent of acetic acid is 0.5% ~ 3%, and the mass percent concentration of the chitosan in described chitosan-acetum is 1% ~ 3%。
In technique scheme, described step 2 Hericium Erinaceus Polysaccharide extracting solution is with the blend step of chitosan-acetum, and the volume ratio of described Hericium Erinaceus Polysaccharide extracting solution and described chitosan-acetum is 40 ~ 60:1。
In technique scheme, in described step 3 first time centrifugal treating step, described first supernatant is at 2 DEG C ~ 6 DEG C and places 2h ~ 3h。
In technique scheme, in described step 3 first time centrifugal treating step, the centrifugal speed of described first time centrifugal treating is 2500r/min ~ 3500r/min, and centrifugation time is 10min ~ 15min。
In technique scheme, in described step 4 second time centrifugal treating step, the second supernatant is at 2 DEG C ~ 6 DEG C and places 2h ~ 3h。
In technique scheme, in described step 4 second time centrifugal treating step, the centrifugal speed of described second time centrifugal treating is 2500r/min ~ 3500r/min, and centrifugation time is 10min ~ 15min。
In technique scheme, in the blend step of described step 2 Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum, described Hericium Erinaceus Polysaccharide extracting solution is with hericium erinaceus fruiting body powder for raw material, then carries out hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating。
In technique scheme, described step 2 Hericium Erinaceus Polysaccharide extracting solution is with the blend step of chitosan-acetum, and the mixing of chitosan-acetum that Hericium Erinaceus Polysaccharide extracting solution prepares with step one is to adopt vortex mixer to mix homogeneously。
Beneficial effects of the present invention:
(1) method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution provided by the invention, relative to Sevage method of the prior art, trichloroacetic acid method, trifluorotrichloroethane method, do not result in the residual of organic solvent, the advantage with safety non-toxic, environmental protection, therefore, the method for removing protein of this Hericium Erinaceus Polysaccharide extracting solution is applicable to the exploitation of food product。It addition, the method for removing protein of this Hericium Erinaceus Polysaccharide extracting solution, relative to the hydrochloric acid method of prior art, sodium-hydroxide method, polysaccharide will not be made to be hydrolyzed, be avoided that glycosidic bond ruptures, and then ensure that structure and the activity of polysaccharide。
(2) method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution provided by the invention, it is utilize chitosan-acetum that Hericium Erinaceus Polysaccharide extracting solution is carried out deproteinization, wherein, the complex scheme of the protein in chitosan-acetum and Hericium Erinaceus Polysaccharide extracting solution is: the chitosan in chitosan-acetum is used for adsorbing conjugated protein matter, and chitosan and protein bound process include electrostatic interaction and hydrophobic interaction；Containing substantial amounts of free amine group and hydroxyl on the strand of chitosan, these free amine groups and hydroxyl are prone to and protein generation cross-linking reaction；Wherein, the protonation of chitosan refers mainly to the protonation of amino in its molecule, under acetic acid existent condition, protonation along with amino, chitosan can show the effect of cationic polyelectrolyte, therefore, it is possible to electronegative protein micelle in adsorbent solution effectively, and then reach Deproteinated purpose。
(3) method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution provided by the invention, the obtained Hericium Erinaceus Polysaccharide extracting solution after deproteinization, its protein content is only 12.3 ~ 12.8%, there is the advantage that protein removal rate is high, wherein, protein removal rate reaches 59.64% ~ 60.58%, higher than the protein removal rate (52% ~ 53.8%) of the Sevage method of prior art。
(4) method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution provided by the invention, has the advantage that polysaccharide loss rate is low, and the loss rate of its Hericium Erinaceus Polysaccharide is 12% ~ 16.5%, well below the polysaccharide loss rate (52% ~ 55%) of the Sevage method of prior art。
(5) method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution provided by the invention, owing to Deproteinated reagent is chitosan-acetum, it makes consumption low, residual quantity is low, and chitosan has the physiologically active such as blood fat reducing, blood sugar lowering, therefore, the advantage that the method for removing protein of this Hericium Erinaceus Polysaccharide extracting solution has safety non-toxic, environmental protection。
(6) method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution provided by the invention, has processing method simple, easily operates, and production cost is low, and can be applicable to the feature of large-scale production, and can be applied to the production of relevant food health promoting product。
Detailed description of the invention
In order to make technical problem solved by the invention, technical scheme and beneficial effect clearly understand, below in conjunction with embodiment, the present invention is further elaborated。Should be appreciated that specific embodiment described herein is only in order to explain the present invention, is not intended to limit the present invention。
Embodiment 1。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；In the present embodiment, in acetum, the concentration of volume percent of acetic acid is 1%, and the mass percent concentration of the chitosan in chitosan-acetum is 2.5%；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: adopt vortex mixer to mix homogeneously chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution, obtain the first mixture；In the present embodiment, the volume ratio of Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum is 50:1；
Wherein, the Hericium Erinaceus Polysaccharide extracting solution of step 2 is with hericium erinaceus fruiting body powder for raw material, then carries out hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is at 4 DEG C and stands 2.5h；In the present embodiment, the first time centrifugal speed of centrifugal treating is 3000r/min, and centrifugation time is 13min；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtains the second supernatant, then the second supernatant is at 4 DEG C and places 2.5h, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization；In the present embodiment, the centrifugal speed of second time centrifugal treating is 3000r/min, and centrifugation time is 13min。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, the protein content 12.5% of the Hericium Erinaceus Polysaccharide extracting solution after its obtained deproteinization。It addition, measure, according to Coomassie Brilliant Blue, the clearance obtaining protein to reach 60.01%, polysaccharide loss rate is only 16.40%, and chitosan residual rate is 0.21%。
Embodiment 2。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；In the present embodiment, in acetum, the concentration of volume percent of acetic acid is 0.5%, and the mass percent concentration of the chitosan in chitosan-acetum is 1%；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: adopt vortex mixer to mix homogeneously chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution, obtain the first mixture；In the present embodiment, the volume ratio of Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum is 40:1；
Wherein, the Hericium Erinaceus Polysaccharide extracting solution of step 2 is with hericium erinaceus fruiting body powder for raw material, then carries out hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is at 2 DEG C and stands 3h；In the present embodiment, the first time centrifugal speed of centrifugal treating is 2500r/min, and centrifugation time is 15min；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtains the second supernatant, then the second supernatant is at 2 DEG C and places 3h, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization；In the present embodiment, the centrifugal speed of second time centrifugal treating is 2500r/min, and centrifugation time is 15min。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, the protein content 12.3 of the Hericium Erinaceus Polysaccharide extracting solution after its obtained deproteinization。It addition, measure, according to Coomassie Brilliant Blue, the clearance obtaining protein to reach 60%, polysaccharide loss rate is 12.77%, and chitosan residual rate is 0.19%。
Embodiment 3。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；In the present embodiment, in acetum, the concentration of volume percent of acetic acid is 3%, and the mass percent concentration of the chitosan in chitosan-acetum is 2%；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: adopt vortex mixer to mix homogeneously chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution, obtain the first mixture；In the present embodiment, the volume ratio of Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum is 60:1；
Wherein, the Hericium Erinaceus Polysaccharide extracting solution of step 2 is with hericium erinaceus fruiting body powder for raw material, then carries out hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is at 6 DEG C and stands 2h；In the present embodiment, the first time centrifugal speed of centrifugal treating is 3500r/min, and centrifugation time is 10min；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtains the second supernatant, then the second supernatant is at 6 DEG C and places 2h, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization；In the present embodiment, the centrifugal speed of second time centrifugal treating is 3500r/min, and centrifugation time is 10min。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, the protein content 12.8% of the Hericium Erinaceus Polysaccharide extracting solution after its obtained deproteinization。It addition, measure, according to Coomassie Brilliant Blue, the clearance obtaining protein to reach 60.58%, polysaccharide loss rate is only 12%, and chitosan residual rate is 0.20%。
Embodiment 4。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；In the present embodiment, in acetum, the concentration of volume percent of acetic acid is 2%, and the mass percent concentration of the chitosan in chitosan-acetum is 3%；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: adopt vortex mixer to mix homogeneously chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution, obtain the first mixture；In the present embodiment, the volume ratio of Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum is 45:1；
Wherein, the Hericium Erinaceus Polysaccharide extracting solution of step 2 is with hericium erinaceus fruiting body powder for raw material, then carries out hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is at 3 DEG C and stands 2.8h；In the present embodiment, the first time centrifugal speed of centrifugal treating is 2800r/min, and centrifugation time is 14min；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtains the second supernatant, then the second supernatant is at 3 DEG C and places 2.8h, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization；In the present embodiment, the centrifugal speed of second time centrifugal treating is 2800r/min, and centrifugation time is 14min。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, the protein content 12.4% of the Hericium Erinaceus Polysaccharide extracting solution after its obtained deproteinization。It addition, measure, according to Coomassie Brilliant Blue, the clearance obtaining protein to reach 59.64%, polysaccharide loss rate is only 16.50%, and chitosan residual rate is 0.21%。
Embodiment 5。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；In the present embodiment, in acetum, the concentration of volume percent of acetic acid is 1.5%, and the mass percent concentration of the chitosan in chitosan-acetum is 1.5%；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: adopt vortex mixer to mix homogeneously chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution, obtain the first mixture；In the present embodiment, the volume ratio of Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum is 55:1；
Wherein, the Hericium Erinaceus Polysaccharide extracting solution of step 2 is with hericium erinaceus fruiting body powder for raw material, then carries out hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is at 5 DEG C and stands 2.3h；In the present embodiment, the first time centrifugal speed of centrifugal treating is 3300r/min, and centrifugation time is 11min；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtains the second supernatant, then the second supernatant is at 5 DEG C and places 2.3h, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization；In the present embodiment, the centrifugal speed of second time centrifugal treating is 3300r/min, and centrifugation time is 11min。
A kind of method for removing protein of the Hericium Erinaceus Polysaccharide extracting solution of the present embodiment, the protein content 12.7% of the Hericium Erinaceus Polysaccharide extracting solution after its obtained deproteinization。It addition, measure, according to Coomassie Brilliant Blue, the clearance obtaining protein to reach 60.55%, polysaccharide loss rate is only 14.70%, and chitosan residual rate is 0.18%。
Finally should be noted that; above example is merely to illustrate technical scheme but not limiting the scope of the invention; although the present invention being explained in detail with reference to preferred embodiment; it will be understood by those within the art that; technical scheme can be modified or equivalent replacement, without deviating from the spirit and scope of technical solution of the present invention。
Claims (9)
1. the method for removing protein of a Hericium Erinaceus Polysaccharide extracting solution, it is characterised in that: it comprises the following steps:
Step one, prepares chitosan-acetum: dissolve the chitosan in acetum, stir, and namely prepares chitosan-acetum；
Step 2, Hericium Erinaceus Polysaccharide extracting solution and the mixing of chitosan-acetum: chitosan-acetum prepared with step one for Hericium Erinaceus Polysaccharide extracting solution is mixed homogeneously, obtains the first mixture；
Step 3, first time centrifugal treating: the first mixture step 2 obtained carries out first time centrifugal treating, obtains the first supernatant, then the first supernatant is under uniform temperature standing certain time；
Step 4, second time centrifugal treating: the first supernatant after standing in step 3 is carried out second time centrifugal treating, obtain the second supernatant, then the second supernatant is under uniform temperature placement certain time, namely prepare the Hericium Erinaceus Polysaccharide extracting solution after deproteinization。
2. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterized in that: described step one is prepared in chitosan-acetum step, in described acetum, the concentration of volume percent of acetic acid is 0.5% ~ 3%, and the mass percent concentration of the chitosan in described chitosan-acetum is 1% ~ 3%。
3. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterized in that: described step 2 Hericium Erinaceus Polysaccharide extracting solution is with the blend step of chitosan-acetum, and the volume ratio of described Hericium Erinaceus Polysaccharide extracting solution and described chitosan-acetum is 40 ~ 60:1。
4. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterised in that: in described step 3 first time centrifugal treating step, described first supernatant is at 2 DEG C ~ 6 DEG C and places 2h ~ 3h。
5. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterized in that: in described step 3 first time centrifugal treating step, the centrifugal speed of described first time centrifugal treating is 2500r/min ~ 3500r/min, and centrifugation time is 10min ~ 15min。
6. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterised in that: in described step 4 second time centrifugal treating step, the second supernatant is at 2 DEG C ~ 6 DEG C and places 2h ~ 3h。
7. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterized in that: in described step 4 second time centrifugal treating step, the centrifugal speed of described second time centrifugal treating is 2500r/min ~ 3500r/min, and centrifugation time is 10min ~ 15min。
8. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterized in that: in the blend step of described step 2 Hericium Erinaceus Polysaccharide extracting solution and chitosan-acetum, described Hericium Erinaceus Polysaccharide extracting solution is with hericium erinaceus fruiting body powder for raw material, then hot water extraction the Hericium Erinaceus Polysaccharide extracting solution obtained after centrifugal treating are carried out。
9. the method for removing protein of a kind of Hericium Erinaceus Polysaccharide extracting solution according to claim 1, it is characterized in that: described step 2 Hericium Erinaceus Polysaccharide extracting solution is with the blend step of chitosan-acetum, and the mixing of chitosan-acetum that Hericium Erinaceus Polysaccharide extracting solution prepares with step one is to adopt vortex mixer to mix homogeneously。
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| CN110922494B (en) * | 2019-11-01 | 2021-09-21 | 福州福德恩生物科技有限公司 | Hericium erinaceus polysaccharide extraction and separation method |
| CN113999324A (en) * | 2021-11-11 | 2022-02-01 | 北京亚东生物制药(安国)有限公司 | Method for deproteinizing hericium erinaceus liquid |
| CN113999324B (en) * | 2021-11-11 | 2022-09-09 | 北京亚东生物制药(安国)有限公司 | Method for deproteinizing hericium erinaceus liquid |
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