CN1869077A - Method of extracting avenabeta glucosan - Google Patents
Method of extracting avenabeta glucosan Download PDFInfo
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- CN1869077A CN1869077A CN 200610089486 CN200610089486A CN1869077A CN 1869077 A CN1869077 A CN 1869077A CN 200610089486 CN200610089486 CN 200610089486 CN 200610089486 A CN200610089486 A CN 200610089486A CN 1869077 A CN1869077 A CN 1869077A
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- oat bran
- avenabeta glucosan
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Abstract
A process for extracting beta-glucosan from oats includes such steps as mixing oats bran with water, stirring at 50-60 deg.C, regulating pH=8-10, adding proteinase, hydrodyzing the protein in oats bran, adding alpha-amylase, treating at 80 deg.C, filtering to obtain clear liquid, ultrafiltering, and freeze-drying.
Description
Technical field
The present invention relates to a kind of method of extracting avenabeta glucosan.
Background technology
Avenabeta glucosan is with β-1,3-, and β-1, the viscosity poly dextran that the 4-glycosidic link couples together, it mainly concentrates on the aleurone layer of oat bran.Avenabeta glucosan has multiple function, can blood fat reducing, controlling blood sugar, strengthening immunity, and anti-ly treat constipation, improve intestinal microenvironment etc., be widely used in medicine and field of health care food.
At present, the preparation avenabeta glucosan is generally all by solvent extraction or employing irenine extraction method.These methods all need to introduce organic solvent, and the cost height is removed difficult; And, in separating protein process, generally adopt the method for isoelectric precipitation, it is low to remove efficient, and it is centrifugal to remove process need, is unfavorable for suitability for industrialized production; Also there is complex steps in these extracting method, and adding reagent is many, the not high shortcoming of products obtained therefrom purity.
Summary of the invention
The purpose of this invention is to provide a kind of method of extracting avenabeta glucosan.
The method of extraction avenabeta glucosan provided by the present invention comprises the steps:
1) oat bran is added water under 50-60 ℃ and stir, regulate pH8-10;
2) add proteolytic enzyme at the 45-60 ℃ of albumen of handling down in the hydrolysis oat bran;
3) add α-Dian Fenmei and handle down, filter, obtain clarified liq at 80 ℃;
4) adopting molecular weight cut-off is the ultrafiltration membrance filter of 30000-50000Da, obtains beta-glucan solution, and lyophilize obtains the beta-glucan product.
Wherein, the particle diameter of the described oat bran of step 1) is preferably the 20-60 order.The weight ratio of described oat bran of step 1) and water is 1: 10-20.
Step 2) proteolytic enzyme is Sumizyme MP; Unit of activity is 50-200 ten thousand U/g, and consumption is 3-5g Sumizyme MP/100g oat bran; Treatment time is 1-4 hour.
The unit of activity of step 3) α-Dian Fenmei is 2000-5000U/g, and consumption is 1-4g α-Dian Fenmei/100g oat bran; Treatment time is 0.5-2 hour.Step 3) is filtered into the employing Plate Filtration.Also be added with diatomite in the Plate Filtration as flocculating aids.
The present invention extracts the method for avenabeta glucosan, does not need organic reagent, and extraction and separation method is easy, and extraction yield is up to 81%, and gained beta-glucan product purity is higher, reaches 85%, and quality product is greatly improved.
Description of drawings
Fig. 1 is the process flow sheet of the beta-glucan of the present invention's extraction;
Fig. 2 is the water content percentage change curve over time of skin surface;
Fig. 3 is the variation diagram of hyaluronic acid contents in the rat skin;
Fig. 4 A and Fig. 4 B are for using the photo of beta-glucan front and back rat skin.
Embodiment
Embodiment 1, extraction beta-glucan
The process flow sheet that extracts beta-glucan as shown in Figure 1, detailed process is as follows:
(1) oat bran is chosen: select for use 20 orders to the oat bran between 100 orders, wherein soluble ss-glucans content is 8.6%.
(2) beta-glucan extracts: reaction vessel adds deionized water, and adjust pH is heated to 50-60 ℃ to about the 8-10, adds oat bran, and the weight ratio of oat bran and water is 1: 20, evenly stirs 1-3 hour.
(3) albumen is removed: extract the about 8.0-8.5 of pH value in the afterreaction container, adding unit of activity is the Sumizyme MP (every 100g oat bran uses the 5g Sumizyme MP) of 1,000,000 U/g, continues to stir 1-2 hour down at 50 ℃.
(4) starch is removed: adding unit of activity is the α-Dian Fenmei (every 100g oat bran uses the 2g Sumizyme MP) of 4000U/g, 80 ℃ of following stir process 0.5 hour, is amylolysis the oligosaccharides of small molecular weight.
(5) utilize flame filter press to filter, add diatomite and help filter, obtain brown clarified liq.
(6) adopting molecular weight cut-off is the brown clarified liq of ultra-filtration membrane uf processing gained of 50000Da, collects the liquid that dams, and obtains the liquid avenabeta glucosan.
(7) lyophilize obtains the avenabeta glucosan product.
The purity of product is 85%, and extraction yield is 81%.
The functional experiment of embodiment 2, avenabeta glucosan product
1, moisture-keeping efficacy experiment
Adopt human test's mode,, choose 20 volunteers with the water content and the TEWL value of moisture tester test skin; Volunteer men and women half and half.Adopt two kinds of different emulsions to be coated on skin surface: one contains 5% oat-beta-glucan (β-glucan), one contains 5% hyaluronic acid (HA); Test once per half an hour, totally 4 hours.The water content percentage change of skin surface over time curve as shown in Figure 2, the result shows: compare with hyaluronic acid, avenabeta glucosan has better long-acting humidity-holding effect.
2, oat-beta-glucan can increase the content of the transparent ester acid of rat skin
2.1 experimental drug
Avenabeta glucosan adopts 1% avenabeta glucosan; Control group adopts 1% hyaluronic acid.
2.2 laboratory animal
40 of cleaning level Wistar rats, male, body weight 220～240g is available from Beijing dimension tonneau China Experimental Animal Center
2.3 instrument and reagent
New Technique Application Inst., Beijing City MB-microplate reader; The hyaluronic acid detection kit is available from Beijing ring Ya Taike biomedical technology company limited.
2.4 modeling and medication
Rat is divided into normal group, control group and avenabeta glucosan group at random, 8 every group, pushes away respectively in the depilation of the ridge both sides of experimental animal rat depilation scope 3cm * 3cm with pet.
During the experiment beginning, do not have the hair-fields in the both sides, back and clean with clear water earlier, choose every rat back both sides then and do not have hair-fields intermediary skin, the about 2cm * 2cm of experimental point, normal group is left intact except that clear water cleans; Control group is coated with isodose control formulation, avenabeta glucosan group and is coated with isodose avenabeta glucosan preparation, once a day.Drew materials in 7 days behind the coating.
2.5 hyaluronic acid contents is measured
Get laboratory animal immediately behind the execution rat and smear position skin, mark right side 2cm * 2cm goes in ring and gets skin, after removing fat, precision weighs weight in wet base, skin is cut for a short time with ophthalmology shred, by 1: 10 (the 1g skin histology adds 10mL physiological saline), move in the glass homogenizer homogenate, constantly cooling in the homogenate process, at 4 ℃ of centrifugal 10min of following 10000rpm, get 100 μ L homogenate supernatant liquor-20 ℃ preservations and be used in reference to target mensuration.
Adopt the ELASA method to measure hyaluronic acid contents in the skin.The result of variations of hyaluronic acid contents such as table 1 and shown in Figure 3 in the rat skin, the result shows: in the avenabeta glucosan group rat skin hyaluronic acid contents apparently higher than normal group, and significant difference; Be higher than control group, significant difference.
The variation of hyaluronic acid contents in the rat skin (x ± s)
| Group | Number of animals (n) | HA(ng/ml) |
| Normal group control group avenabeta glucosan group | 8 8 8 | 183.13±20.29 201.57±34.77 257.42±55.48 **# |
Compare with normal group, * P＜0.05, #P＜0.05, ##P＜0.01 are compared with control group in * * P＜0.01
Use the skin texture survey meter to measure skin texture, relatively use avenabeta glucosan and do not use the avenabeta glucosan skin texture to change, use avenabeta glucosan preceding skin photo such as Fig. 4 A, use skin photo such as Fig. 4 B of avenabeta glucosan after one week, test shows uses avenabeta glucosan after one week, and skin texture is greatly improved.
Claims (7)
1, a kind of method of extracting avenabeta glucosan comprises the steps:
1) oat bran is added water under 50-60 ℃ and stir, regulate pH8-10;
2) add proteolytic enzyme at the 45-60 ℃ of albumen of handling down in the hydrolysis oat bran;
3) add α-Dian Fenmei and handle down, filter, obtain clarified liq at 80-100 ℃;
4) adopting molecular weight cut-off is the ultrafiltration membrance filter of 30000-50000Da, obtains beta-glucan solution, and lyophilize obtains the beta-glucan product.
2, method according to claim 1 is characterized in that: the particle diameter of the described oat bran of step 1) is the 20-100 order.
3, method according to claim 1 is characterized in that: the weight ratio of described oat bran of step 1) and water is 1: 10-20.
4, method according to claim 1 is characterized in that: step 2) proteolytic enzyme is Sumizyme MP; Unit of activity is 50-200 ten thousand U/g, and consumption is 3-5g Sumizyme MP/100g oat bran; Treatment time is 1-4 hour.
5, method according to claim 1 is characterized in that: the unit of activity of step 3) α-Dian Fenmei is 2000-5000U/g, and consumption is 1-4g α-Dian Fenmei/100g oat bran; Treatment time is 0.5-2 hour.
6, method according to claim 1 is characterized in that: step 3) is filtered into the employing Plate Filtration.
7, method according to claim 6 is characterized in that: also be added with diatomite in the Plate Filtration as flocculating aids.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101798585A (en) * | 2010-04-08 | 2010-08-11 | 天津科技大学 | Method for high-yield production of oat polysaccharide with naked oat bran as raw material |
| CN101805774A (en) * | 2010-03-12 | 2010-08-18 | 南通康麦生物科技有限公司 | Method for comprehensively extracting oat polypeptide and oat glucan |
| CN101120776B (en) * | 2007-06-23 | 2011-04-06 | 西藏天麦力健康品有限公司 | Method for extracting beta-glucan from cereal bran using membrane separation technology |
| CN101649003B (en) * | 2009-09-09 | 2011-06-15 | 中国农业科学院农产品加工研究所 | Method for extracting beta-hyskon, starch, protein and grease from oat |
| CN102247306A (en) * | 2010-05-18 | 2011-11-23 | 北京工商大学 | Oat extract and preparation method and utilization thereof |
| CN102603915A (en) * | 2012-03-13 | 2012-07-25 | 无锡德冠生物科技有限公司 | Method for extracting oat beta-glucan |
| CN102911287A (en) * | 2012-11-21 | 2013-02-06 | 福建科宏生物工程有限公司 | Method for preparing bamboo leaf polysaccharides from bamboo pulp |
| CN104774886A (en) * | 2015-03-17 | 2015-07-15 | 广州中康食品有限公司 | Preparation method of allergen-free oat beta-glucan |
| GB2567501A (en) * | 2017-10-13 | 2019-04-17 | Tate & Lyle Sweden Ab | Beta-glucan compositions |
| CN110655588A (en) * | 2019-10-10 | 2020-01-07 | 上海辉文生物技术股份有限公司 | Preparation method and application of Cladosiphon okamuranus polysaccharide |
| CN110790850A (en) * | 2019-11-21 | 2020-02-14 | 吉林工程技术师范学院 | Method for extracting β -glucan from oat bran |
| CN114805630A (en) * | 2022-03-07 | 2022-07-29 | 广州科斯曼生物科技有限公司 | Oat glucan and preparation method and application thereof |
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| CN101857646B (en) * | 2010-05-27 | 2011-10-26 | 山西金绿禾燕麦研究所 | Method for extracting high-purity beta-glucan and whole oat flour from oat bran |
Family Cites Families (3)
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| ATE372061T1 (en) * | 1998-10-26 | 2007-09-15 | Oatly Ab | METHOD FOR ISOLATING A BETA-GLUCAN COMPOSITION FROM OATS AND PRODUCTS MADE THEREFROM |
| JP2006524994A (en) * | 2003-03-27 | 2006-11-09 | ザ ガバナーズ オブ ザ ユニバーシティー オブ アルバータ | Method for producing high viscosity beta-glucan concentrate |
| CN1300323C (en) * | 2003-06-26 | 2007-02-14 | 中国农业科学院农产品加工研究所 | Process for preparing oat-beta glucan |
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- 2006-06-29 CN CNB2006100894866A patent/CN100393750C/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101120776B (en) * | 2007-06-23 | 2011-04-06 | 西藏天麦力健康品有限公司 | Method for extracting beta-glucan from cereal bran using membrane separation technology |
| CN101649003B (en) * | 2009-09-09 | 2011-06-15 | 中国农业科学院农产品加工研究所 | Method for extracting beta-hyskon, starch, protein and grease from oat |
| CN101805774A (en) * | 2010-03-12 | 2010-08-18 | 南通康麦生物科技有限公司 | Method for comprehensively extracting oat polypeptide and oat glucan |
| CN101805774B (en) * | 2010-03-12 | 2013-04-17 | 南通康麦生物科技有限公司 | Method for comprehensively extracting oat polypeptide and oat glucan |
| CN101798585A (en) * | 2010-04-08 | 2010-08-11 | 天津科技大学 | Method for high-yield production of oat polysaccharide with naked oat bran as raw material |
| CN102247306B (en) * | 2010-05-18 | 2013-07-10 | 北京工商大学 | Oat extract and preparation method and utilization thereof |
| CN102247306A (en) * | 2010-05-18 | 2011-11-23 | 北京工商大学 | Oat extract and preparation method and utilization thereof |
| CN102603915A (en) * | 2012-03-13 | 2012-07-25 | 无锡德冠生物科技有限公司 | Method for extracting oat beta-glucan |
| CN102911287A (en) * | 2012-11-21 | 2013-02-06 | 福建科宏生物工程有限公司 | Method for preparing bamboo leaf polysaccharides from bamboo pulp |
| CN104774886A (en) * | 2015-03-17 | 2015-07-15 | 广州中康食品有限公司 | Preparation method of allergen-free oat beta-glucan |
| GB2567501A (en) * | 2017-10-13 | 2019-04-17 | Tate & Lyle Sweden Ab | Beta-glucan compositions |
| CN110655588A (en) * | 2019-10-10 | 2020-01-07 | 上海辉文生物技术股份有限公司 | Preparation method and application of Cladosiphon okamuranus polysaccharide |
| CN110790850A (en) * | 2019-11-21 | 2020-02-14 | 吉林工程技术师范学院 | Method for extracting β -glucan from oat bran |
| CN114805630A (en) * | 2022-03-07 | 2022-07-29 | 广州科斯曼生物科技有限公司 | Oat glucan and preparation method and application thereof |
| CN114805630B (en) * | 2022-03-07 | 2022-11-08 | 广州科斯曼生物科技有限公司 | Oat beta-glucan and preparation method and application thereof |
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| CN100393750C (en) | 2008-06-11 |
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Owner name: BEIJING SANYOU HUIZHI BIOTECHNOLOGY CO.LTD. Free format text: FORMER OWNER: BEIJING TECHNOLOGY AND BUSINESS UNIEERSITY Effective date: 20070518 |
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Effective date of registration: 20070518 Address after: 102200, No. 5, super Road, Changping District Economic Development Zone, Beijing Applicant after: Beijing Sanyou Huizhi Biological Technology Co., Ltd. Address before: 100037, Fu Cheng Road, Beijing, Haidian District, No. 33 Applicant before: Beijing Technology and Business University |
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Assignee: Beijing Brilliance Biochemical Co., Ltd. Assignor: Beijing Sanyou Huizhi Biological Technology Co., Ltd. Contract record no.: 2010990000568 Denomination of invention: Method for extracting oat beta-glucan Granted publication date: 20080611 License type: Exclusive License Open date: 20061129 Record date: 20100730 |
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Assignee: Beijing Brilliance Biochemical Co., Ltd. Assignor: Beijing Sanyou Huizhi Biological Technology Co., Ltd. Contract record no.: 2010990000568 Date of cancellation: 20110728 |