KR20110003872A - Cosmetic composition for anti-oxidation and whitening - Google Patents

Abstract

PURPOSE: A cosmetic composition containing complex plant fermentation extract with antioxidation and whitening is provided to enhance skin permeability and efficiency. CONSTITUTION: A cosmetic composition for antioxidation and whitening contains mixture fermentation of green tea, Morus alba, and glycyrrhizae radix and complex plant fermentation extract as active ingredients. The mixture fermentation of green tea, Morus alba, and glycyrrhizae radix is obtained by mixing the green tea, Morus alba, and glycyrrhizae radix in a same weight ratio. The mixture fermentation of green tea, Morus alba, and glycyrrhizae radix is obtained by fermenting with Bacillus subtilis. The cosmetic composition is used in the form of skin softener, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, baby lotion or baby cream.

Description

Cosmetic composition for antioxidant and whitening {Cosmetic Composition for Anti-oxidation and Whitening}

The present invention contains a multi-fermented plant fermentation extract having high antioxidant and whitening ability obtained by hot water extraction of fermented ginseng mixed with green tea, lettuce, and licorice fermented with Bacillus bacteria and bifidus bacteria as an active ingredient. It relates to a cosmetic composition.

With the rapid industrialization, air pollution and destruction of the ozone layer increase the amount of UV, which promotes human skin aging and pigmentation.

Specifically, skin aging is progressed by free radicals caused by various causes both inside and outside the body. The free radicals are chemically highly reactive chemicals, which are caused by external factors such as ultraviolet rays, radiation, exhaust gases, stress, and the like. It is produced in the human body by metabolic processes such as mitochondria and white blood cells in the body, and it causes skin aging by peroxidation of lipids, which are components of cell membranes, through a multi-step chain reaction. Most of the free radicals associated with skin aging are Reactive Oxygen Species (ROS), which are super oxide radicals, hydrogen peroxide, hydroxy radicals, and single-state oxygen (ROS). Singlet oxygen). Therefore, since free radical type oxygen or reactive oxygen species are highly related to skin aging, conventionally, various antioxidants such as superoxide dismutase, vitamin E derivatives, vitamin C and derivatives thereof, flavonoids, and the like are contained in cosmetics to prevent skin aging. There have been many efforts to achieve the effect. However, when blending the above components in cosmetics, such as safety and stability problems it was difficult to expect a substantial effect.

On the other hand, there are many causes of pigmentation that are problematic with skin aging, but the main cause is due to ultraviolet rays. When the skin is exposed to ultraviolet rays, melanin is synthesized and released in melanocytes, a kind of skin cells, and the skin epidermis becomes black. do. In the process of producing melanin, tyrosinase produces dopaquinone, which acts as a substrate of tyrosine in melanocytes, and by continuous oxidation, dopaquinone passes through dopachrome and 5, 6-dihydroxy, a monomer of melanin. Indole and 5, 6-dihydroxyindole-2-carboxylic acid are produced, and melanin which is a black pigment is produced by copolymerization thereof. Therefore, if the activity of tyrosinase is inhibited during melanin production, the pigmentation caused by melanin production can be reduced and skin whitening effect can be obtained. Therefore, studies on substances that inhibit the activity of tyrosinase are actively conducted to obtain skin whitening effect. As tyrosinase activity inhibiting substances known to date, kojic acid, arbutin, vitamin C, plant extracts, and the like, there are problems such as safety and stability when formulating the above ingredients in cosmetics.

In recent years, the scientific trend has revealed that there are many physiologically active substances that have anti-aging, anti-wrinkle, antioxidant or whitening ability in various natural plants including herbal medicines such as ginseng. Under the influence of (LOHAS), natural cosmetics and herbal cosmetics are in the spotlight, and thus extracts of various plants have been used in the manufacture of these cosmetics.

First, Panax ginseng belonging to the Ogapiaceae in plant taxonomy ginseng ) has been used as a panacea for a long time due to its various effects, and in recent years, scientific research has been used to improve diseases related to geriatric diseases such as lowering cholesterol, strengthening blood pressure, increasing cardiac function, cerebrovascular expansion, and anti-diabetes and anti-cancer, anti-diabetes. It has been found to exhibit various pharmacological effects such as allergy, antioxidant, immunomodulation, etc., and recently, anti-aging effect is expected and is also used as an herbal cosmetic raw material. In addition, the most representative active ingredient of ginseng exhibiting various physiological activities is saponin. Saponins are ginsenosides combined with glucose, aribinose, rhamnose, and the like. Fermentation using the same probiotics is known to increase the content of sugar as well as to produce saponins in a reduced form (Patent No. 10-99094, US Patent No. 592537).

On the other hand, green tea is heat-treated by steam steaming the leaves of tea tree, which is evergreen tree, and dried and used as tea, which is known to inactivate oxidase through heat treatment. Green tea is known to have high antioxidant potential due to carotenoids, vitamin E, and various forms of catechins, as well as various flavonoid glycosides. On the other hand, flavonoid glycosides have been reported to be converted to phenolic acid by enteric bacteria (Kim D-H, Natural Product Science, 8, 35-43, 2002).

Also, Morus bark is Morus alba . It is the root bark of the leaves of the big leaves of the leaves, the mulberry, the wild mulberry, and the crayfish. It contains a physiological substance called mulberroside. These skins are known to have antitussive effect, diuretic effect, anti-allergic effect, fever and headache, and it is also effective for skin itching and when used as a cosmetic, it is known to have excellent whitening effect (Patent Application No. 1997-33567). . In particular, conversion of the glycoside-type mulberroside to the nonglycoside Moracin has been reported to increase the whitening effect (Lee SH et al, Biol Pharm Bull, 25, 1045-1048, 2002). .

And licorice ( Glycyrrhiza uralensis ) has various flavonoids such as glycyrrhizin, liquiritigenin, and liquiritin as physiologically active substances. In particular, glycyrrhizin, a representative physiologically active substance, is converted to glycyrrhetinic acid after fermentation through intestinal bacteria, thereby increasing the absorption rate or physiological activity effect (Kim DH, Natural Product Science, 8). , 35-43, 2002).

Accordingly, the invention is green tea, licorice and Mori Cortex the Bacillus subtilis (Bacillus described above subtilis ) Antioxidant and whitening cosmetic composition containing as a active ingredient a mixed fermentation extract fermented with strain and fermented ginseng fermented with Bifidobacteria (product of Bifido) The purpose is to provide.

In order to achieve the above object, the present invention provides a cosmetic composition for antioxidant and whitening, characterized in that it comprises a mixed fermentation extract of green tea and the combination of fermented ginseng and licorice, and a complex plant fermentation extract extracted by combining the fermented ginseng as an active ingredient. do.

In addition, the composite plant fermentation extract is provided in an amount of 0.001 to 20% by weight based on the total cosmetic composition.

In addition, the complex plant fermentation extract (a) preparing a fermentation raw material by mixing green tea, lettuce and licorice; (b) fermenting the fermentation raw material with Bacillus to prepare a mixed fermentation product; And (c) combining and extracting the fermented ginseng fermented with Bifidobacteria and the mixed fermentation product to provide an antioxidant and whitening cosmetic composition.

The mixed plant fermentation extract extracted from the mixed fermentation of green tea, lettuce and licorice according to the present invention, and the fermented ginseng, during the fermentation process, compared to the conventional cosmetic composition prepared using conventional unfermented plant extracts In addition to the various bioactive substances produced, glycosides are converted to the non-glycoside type, thereby increasing skin absorption and efficacy, and thus high antioxidant and whitening effects are expressed.

Hereinafter, a complex plant fermented extract having an antioxidant and whitening effect according to the present invention, a preparation method thereof, and a cosmetic composition using the same will be described in more detail. On the other hand, in order to help the understanding of the present invention, the preparation examples of the composite plant fermentation extract and the preparation examples, experimental examples of the cosmetic composition according to the present invention, but these examples, preparation examples, etc. It is merely an example and the scope of the present invention is not limited thereto.

First, the composite plant fermented extract according to the present invention is fermented by adding alcohol extracts of green tea, lettuce, licorice to the whole culture medium using the Bacillus bacillus, CKJ-Shebah1, as a seed. The freeze-dried mixed fermentation powder was mixed 1: 1 with fermented ginseng (Bifido) powder fermented with Bifidobacteria, and autoclaved at 120 ° C. for 15 minutes by adding 10 times the weight of the compound. After extracting at 60 ° C. for 3 hours to obtain an extract in the form of a dry residue after freezing, it is obtained by dissolving it in a solvent. The manufacturing process of the complex plant fermentation extract according to the present invention is described in more detail through the following examples. Is the same as

EXAMPLES Preparation of Complex Plant Fermented Extract

Specifically, after mixing the baekbaekpi, licorice, green tea powder in the same weight ratio, 80% ethanol (v / v, ethanol / water) per 100g is added in a ratio of 1:10 (mixture: 80% ethanol) to the weight of room temperature It is extracted by stirring for 3 days. In addition, the extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor to remove ethanol, and the remaining solution was frozen at −70 ° C. for 3 hours, and then freeze-dried at −50 to −10 ° C. for 24 hours. Prepare a mixed extract powder of green tea.

Subsequently, Bacillus subtilis was incubated in nutrient broth (3 grams of beef extract per liter of water, 5 grams of peptone) for 24 hours at 38 to 40 ° C. Mix with). At this time, the mixed extract powder of lettuce, green tea, licorice in the total culture solution is mixed to 0.5-2% of the total weight, and cultured liquid at 38-40 ° C. for 24 hours, and then the culture medium is frozen at -70 ° C. for 3 hours and then -50 Freeze-drying at -10 ° C for 24 to 72 hours to prepare a mixed fermentation extract.

In addition, the mixed fermented extract prepared as described above and the fermented ginseng powder prepared in Bifido Co., Ltd. are blended in the same amount, and 10 times the weight of the compound is added and autoclaved at 120 ° C. for 15 minutes, followed by 3 hours at 60 ° C. After extraction, the compound is fermented to obtain a dry residue.

Therefore, the complex plant fermentation extract obtained as described above is contained in the antioxidant and whitening cosmetic composition according to the present invention in an amount of 0.001 to 20%, preferably 0.01 to 10% (in terms of dry residue), the content of which is 0.001 If it is less than the weight%, it is difficult to expect the distinct effect of antioxidant and whitening.

In addition, looking at the preparation of the antioxidant and whitening cosmetic composition according to the present invention containing the complex plant fermentation extract as follows. These preparations can be prepared by methods known in the art, while the cosmetic composition can be prepared with a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, infant lotion, infant cream In addition, the formulation is not particularly limited, and the scope of the present invention is not limited to the respective compositions and contents shown below.

Preparation Example Antioxidant and Whitening Cosmetic Composition Containing Complex Plant Fermented Extract According to the Present Invention

Preparation Example 1-Lotion Formulation

number Raw material Formulation Example 1 (g) Comparative Formulation Example 1-1 (g) Comparative Formulation Example 1-2 (g) One Combined Plant Fermentation Extract
(10%) solution 50.0 25.0 - 2 Distillate 110.0 135.0 160.0 3 Jojoba 16.0 16.0 16.0 4 Macadamia Nut 16.0 16.0 16.0 5 IPM 1.4 1.4 1.4 6 Dimethicone 1.4 1.4 1.4 7 Squalene 8.0 8.0 8.0 8 Olive Oil Wax 16.0 16.0 16.0 9 Sicily Alcohol 2.0 2.0 2.0 11 Hyaluronic acid 20.0 20.0 20.0 12 Panthenol 4.0 4.0 4.0 13 Preservative a very small amount a very small amount a very small amount

Preparation Example 2-Cream

number Raw material Formulation Example 2 (g) Comparative Formulation Example 2-1 (g) Comparative Formulation Example 2-2 (g) One Combined Plant Fermentation Extract
(10%) solution 50.0 25.0 - 2 Distillate 280.0 305.0 330.0 3 Water emulsion 4.0 4.0 4.0 4 Hyaluronic acid 4.0 4.0 4.0 5 Olive oil 12.0 12.0 12.0 6 Rice bran oil 13.0 13.0 13.0 7 Jojoba 20.0 20.0 20.0 8 Macadamia Nut 12.0 12.0 12.0 9 Montanav 32.0 32.0 32.0 10 Squalene 14.0 14.0 14.0 11 IPM 4.0 4.0 4.0 12 Dimethicone 4.0 4.0 4.0 13 Sicily Alcohol 4.0 4.0 4.0 14 Preservative a very small amount a very small amount a very small amount

(Production Example 3-Baby Lotion Formulation)

number Raw material Formulation Example 3 (g) Comparative Formulation Example 3-1 (g) Comparative Formulation Example 3-2 (g) One Combined Plant Fermentation Extract
(10%) solution 50.0 25.0 - 2 Distillate 104.0 139.0 154.0 3 Fermented golden 80.0 80.0 80.0 4 Water emulsion 4.0 4.0 4.0 5 Hyaluronic acid 40.0 40.0 40.0 6 Panthenol 8.0 8.0 8.0 7 Jojoba 20.0 20.0 20.0 8 Squalene 8.0 8.0 8.0 9 Ceramide 40.0 40.0 40.0 10 Shea Butter 10.0 10.0 10.0 11 IPM 2.0 2.0 2.0 12 Dimethicone 2.0 2.0 2.0 13 Avocado Oil 20.0 20.0 20.0 14 Olive Oil Wax 32.0 32.0 32.0 15 Cetyl alcohol 4.0 4.0 4.0 16 Preservative a very small amount a very small amount a very small amount

 Experimental Example Measurement of Antioxidant and Whitening Activity

In addition, the antioxidant and whitening ability of the mixed plant fermentation extract extracted from the mixed fermentation product of green tea and lettuce and licorice and fermented ginseng (Bifido) according to the present invention as a sample is as follows. .

Experimental Example 1-Antioxidant Capacity

Antioxidant activity was measured by DPPH free radical scavenging method. After dissolving samples of various concentrations of the complex plant fermentation extract according to the present invention in water, 3 ml of 1.5x10-4M / EtOH solution was added, and the absorbance was measured at 517 nm after standing at room temperature for 20 minutes. Antioxidant activity was expressed as radical scavenging ability according to the degree of decreasing the absorbance of the control group without addition of the sample (Table 1).

Radical Scavenging Capacity (%) = 100-B / A × 100

A: absorbance of the control group without addition of the sample

B: absorbance after adding sample

Table 1 : Radical Scavenging Activity

Sample concentration Radical scavenging ability 0.10% 22.20% 0.25% 47.40% 0.50% 79.50% 0.75% 100% 1.00% 100% 2.00% 100%

(Example 2-Whitening ability)

On the other hand, the whitening ability was examined by the activity inhibitory activity of tyrosinase. Samples of various concentrations of the complex plant fermentation extract according to the present invention were dissolved in water and mixed with L-tyrosine (Sigma) solution. After the tyrosinase (Sigma) enzyme solution was added to the mixed solution and reacted at 37 ° C for 30 minutes. And the extract sample solution was not added to the L-tyrosine solution was carried out in the same manner as the control method. After absorbance of the mixed solutions was measured at 490 nm, the tyrosinase activity inhibitory activity of the extract sample was converted using the following formula and shown in Table 2.

% Tyrosinase activity inhibitory activity = (absorbance of 1-test group / absorbance of control group) X100

Table 2 : Inhibitory Activity of Tyrosinase Activity

Sample concentration Inhibitory activity of tyrosinase activity 0.5% 23.60% 1.0% 47.40% 2.0% 86.50% 3.0% 100% 4.0% 100%

Experimental Example 3-Evaluation of Antioxidant and Whitening Efficacy of Cosmetic Composition

In addition, the antioxidant and whitening efficacy of the antioxidant and whitening cosmetic composition containing the composite plant fermentation extract prepared according to Preparation Example 1 and Preparation Example 2 of the present invention was evaluated through practical use.

A panel of 15 healthy women in their 30s to 40s was applied to the face twice a day with 0.5 g of each lotion (Formulation Example 1) and Cream (Formulation Example 2) prepared in Preparation Examples 1 and 2 after washing the face. The skin antioxidant and whitening effects were measured. And Comparative Formulation Example 1-2 and Comparative Formulation Example 2-2 of Preparation Example 1 and Preparation Example 2 were applied to the face under the same conditions to measure the skin antioxidant and whitening effect. Each application period was 2 weeks and then the results were evaluated. Of the 15 panelists, 12 panelists evaluated that the effects of Formulation Example 1 and Formulation 2 were high, and 80% of the panelists preferred the antioxidant and whitening cosmetic composition containing the complex plant fermentation extract according to the present invention.

Claims (10)

Antioxidant and whitening cosmetic composition, characterized in that it comprises a mixed fermentation extract of green tea, baekbaekpi and licorice, and a mixed plant fermentation extract extracted by combining the fermented ginseng. The method of claim 1, The mixed fermentation product of green tea, lettuce and licorice, antioxidant and whitening cosmetic composition, characterized in that the fermentation by mixing the green tea, lettuce and licorice in the same weight ratio. The method of claim 1, The mixed fermentation product of green tea, lettuce and licorice is an antioxidant and whitening cosmetic composition, characterized in that fermented with Bacillus subtilis strain. The method of claim 1, The complex plant fermentation extract is an antioxidant and whitening cosmetic composition, characterized in that the extract by mixing the fermented ginseng and fermented ginseng mixed with the same weight ratio of green tea and lettuce. The method of claim 1, The complex plant fermentation extract is an antioxidant and whitening cosmetic composition, characterized in that 0.001 to 20% by weight relative to the total cosmetic composition. The method of claim 1, The cosmetic composition is an antioxidant and whitening cosmetic composition, characterized in that the formulation of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, baby lotion or baby cream. The method of claim 1, The complex plant fermentation extract is prepared by (a) mixing the green tea, lettuce and licorice to prepare a fermentation raw material; (b) Bacillus subtilis ( Bcillus subtilis) subtilis ) to prepare a mixed fermentation; And (c) combining and extracting the fermented ginseng fermented with bifidus and the mixed fermentation product. The method of claim 7, wherein The fermentation raw material of step (a) is an antioxidant and whitening cosmetic composition, characterized in that obtained by mixing green tea, baekbaekpi and licorice powder in the same weight ratio and adding 10 times the weight of ethanol as an extraction solvent and stirring. The method of claim 7, wherein The fermentation by Bacillus subtilis of step (b) is mixed with 0.5 ~ 2% of the total culture weight of fermentation raw materials mixed with green tea, lettuce and licorice, antioxidants, characterized in that carried out for 24 hours at 38 ~ 40 ℃ And a cosmetic composition for whitening. The method of claim 7, wherein The step (c) is a combination of fermented ginseng and fermented ginseng of green tea, lettuce and licorice in the same amount, and antioxidant and whitening cosmetic composition, characterized in that the hydrothermal extraction by adding water of 10 times the weight of the blend.