KR20150030092A - Composition for skin whitening containing Panax ginseng polysaccharides and Green tea polysaccharides - Google Patents

Abstract

The present invention relates to a skin whitening composition containing panax ginseng polysaccharides and green tea polysaccharides, and more specifically, to a composition which provides a synergy effect by mixing panax ginseng polysaccharides and green tea polysaccharides, shows excellent skin whitening effect than when using the components respectively, alleviates pigmentation such as freckle or the like or darkness of skin, and makes skin color uniform and bright.

Description

TECHNICAL FIELD The present invention relates to a skin whitening composition containing ginseng polysaccharide and green tea polysaccharide,

The present invention relates to a whitening composition comprising a ginseng polysaccharide and a green tea polysaccharide, more specifically, by using a mixture of a ginseng polysaccharide and a green tea polysaccharide, a synergistic effect can be provided. The present invention relates to a composition which improves pigmentation symptoms such as spots and freckles or dullness of the skin and makes the skin color uniform and bright.

Human skin color is determined by various factors such as the activity of melanocyte making melanocyte, the distribution of blood vessels, the thickness of skin, and the presence or absence of pigment inside and outside the body, such as carotenoids and bilirubin. The most important factor is melanin, a melanin produced by various enzymes such as tyrosinase in melanocytes. The formation of melanin pigment is affected by environmental factors such as genetic factors, hormonal secretion, physiological factors such as stress, and ultraviolet irradiation. Melanin is present in the skin and protects the body from ultraviolet rays, but it is known that melanin is overproduced, thereby promoting pigmentation and aging of the skin and leading to skin cancer induction. (Ascorbic acid, kojic adcid, arbutin, hudroquinone) have been previously used to treat or relieve excessive melanin pigmentation caused by skin pigmentation abnormalities and ultraviolet exposure. Glutathione or a derivative thereof and a substance having inhibitory activity against tyrosinase have been used in cosmetics or medicines in combination with insufficient whitening effect, safety problems to the skin, Its use is restricted due to stability problems and so on.

Accordingly, the inventors of the present invention found that, when a ginseng polysaccharide and a green tea polysaccharide are simultaneously used, they provide a superior skin whitening effect as compared with the case of using each of these substances by a synergistic effect, .

Accordingly, it is an object of the present invention to provide a composition exhibiting excellent skin whitening effect by using a ginseng polysaccharide and a green tea polysaccharide simultaneously.

In order to accomplish the above object, the present invention provides a whitening skin external composition or pharmaceutical composition containing a ginseng polysaccharide and a green tea polysaccharide as an active ingredient.

The composition of the present invention provides an excellent skin whitening effect by containing a ginseng polysaccharide and a green tea polysaccharide, thereby reducing pigmentation symptoms such as spots and freckles, improving skin dullness, and making skin color uniform and bright.

The composition of the present invention contains a ginseng polysaccharide and a green tea polysaccharide as an effective ingredient, thereby providing an excellent skin whitening effect.

The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. In addition, arabinose, rhamnose, glucose and And galactose constitute side chains. The ginseng used in the present invention is a plant belonging to the genus Ogawa and Ginseng. In the present invention, ginseng can be used without limitation in its kind and year-round, and both dried and non-dried ginseng can be used. In the present invention, ginseng can be used without limitation in the site, and specifically, roots of ginseng can be used.

The green tea polysaccharide used in the present invention is derived from green tea and is an acidic polysaccharide group, unlike a polysaccharide found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis. In the present invention, green tea can be used regardless of the type and the number of days of cultivation, and both dried and non-dried green tea can be used. In the present invention, the green tea can be used without limitation in the area, and specifically, the leaves of green tea can be used.

The ginseng polysaccharide and the green tea polysaccharide used in the present invention can be produced by a method known in the art, and the method is not particularly limited.

Specifically, a green tea polysaccharide or a ginseng polysaccharide can be obtained by preparing a ginseng root or green tea leaf extract with water at 38 to 42 캜, concentrating it, removing the impurities with a filter, adding ethanol dropwise thereto and drying with hot air .

The composition of the present invention may contain the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.005 to 9.5% 0.09 to 7.5 wt.%, 0.1 to 6.5 wt.%, 0.3 to 6 wt.%, 0.5 to 5.5 wt.%, Or 0.7 By weight to 5% by weight.

In the present invention, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. In order to maximize the synergistic effect of the combination of the two components, preferably, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1 .

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Reference Example 1] Preparation of ginseng polysaccharide

1 kg of dried white ginseng was pulverized with a blender, and then 10 L of purified water was added thereto, followed by stirring at 38 to 42 ° C in hot water for 24 hours, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 65 g of ginseng polysaccharide.

[Reference Example 2] Production of green tea polysaccharide

1 kg of first processed green tea leaves were pulverized with a blender, 10 l of purified water was added thereto, and the mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise and the mixture was hot-air dried to obtain 72 g of a green tea polysaccharide.

[Example 1] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Reference Example 1 and the green tea polysaccharide prepared in Reference Example 2 were mixed at a ratio of 1: 3.

[Example 2] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Reference Example 1 and the green tea polysaccharide prepared in Reference Example 2 were mixed at a ratio of 1: 1.

[Example 3] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Reference Example 1 and the green tea polysaccharide prepared in Reference Example 2 were mixed at a ratio of 3: 1.

Test Example 1: Effect of tyrosinase inhibition

The tyrosinase enzyme was extracted from Mushroom and was obtained from Sigma (SIGMA). First, the substrate, tyrosine, is dissolved in distilled water to make a 0.3 mg / ml solution. The solution is put into a test tube in an amount of 1.0 ml. Then, 1.0 ml of potassium phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water .

0.2 ml of the sample solution prepared by mixing the mixture of the ginseng polysaccharide of Examples 1 and 3 and the green tea polysaccharide or the ginseng polysaccharide of Reference Examples 1 and 2 and the green tea polysaccharide in the ethanol solution was added to the reaction solution at 37 ° C The reaction was carried out in a thermostat for 10 minutes. As a negative control, 0.2 ml of a solvent was used instead of each sample solution, and ascorbic acid was used as a positive control. 0.1 ml of tyrosinase solution of 2500 units / ml was added to the reaction solution, followed by reaction at 37 ° C for 10 minutes. The reaction tube was immersed in ice water to quench the reaction, and the absorbance at 475 nm was measured with a photoelectron spectrometer. The results are shown in Table 1 below. Each tyrosinase inhibitory effect was calculated by the following equation (1).

Test substance Inhibition rate of tyrosinase (%) Control group (no addition) 0 Ascorbic acid 52 Example 1 52 Example 2 53 Example 3 57 Reference Example 1 48 Reference Example 2 45

In the results shown in Table 1, Reference Examples 1 and 2 using each of the green tea polysaccharides of the ginseng polysaccharide showed inhibitory effects on tyrosinase, but in the case of Examples 1 to 3 in which these polysaccharides were mixed, 1 < / RTI > and < RTI ID = 0.0 > 2, < / RTI >

[Test Example 2] Inhibitory effect on melanin formation by B16 / F10 melanoma cells

A mixture of the ginseng polysaccharide of Examples 1 to 3 and the green tea polysaccharide or a sample containing 0.1% by weight of each of the ginseng polysaccharide and the green tea polysaccharide of Reference Examples 1 and 2 was used as a test substance and B16 / F10 melanoma cells (Korean Cell Line Bank), cultured for 3 days, the culture solution was removed, washed with PBS, and the cells were dissolved with 1N NaOH and absorbance was measured at 405 nm. Cells to which the test substance was not added were used as a control group, and the melanin formation inhibition degree of each test substance was measured by comparing with the melanin content in the control group. The inhibition rate of melanin production was calculated according to Equation (2), and the results are shown in Table 2.

Test substance Melanin inhibition rate (%) Control group (no addition) 0 Kojic acid 53 Example 1 53 Example 2 58 Example 3 60 Reference Example 1 49 Reference Example 2 48

In the results of the above Table 2, Reference Examples 1 and 2 using each of the green tea polysaccharides of the ginseng polysaccharide also exhibited melanin production inhibitory effects, but in the case of Examples 1 to 3 in which these polysaccharides were mixed, It can be seen that a remarkably high melanin production inhibitory effect is exhibited as compared with Examples 1 and 2, and an excellent whitening effect can be provided.

[Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3]

Nutritive creams were prepared in a conventional manner according to the composition of Table 3 below (unit: wt%).

Compounding ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 compare
Formulation Example 1 compare
Formulation Example 2 compare
Formulation Example 3 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Example 1 0.1 - - - - - Example 2 - 0.1 - - - - Example 3 - - 0.1 - - - Reference Example 1 - - - 0.1 - - Reference Example 2 - - - - 0.1 - Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 6.00 6.00 Preservative, incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

[Test Example 3] Skin tone improvement effect

To examine the effect of green tea polysaccharide of ginseng polysaccharide on the skin tone improvement, 60 subjects were divided into 6 groups of 10, and subjects of each group used the above Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 (evening / Day), and the degree of skin tone improvement was evaluated using Facial Stage DM-3 (Moritex, Japan). The improvement rate of the skin tone was determined by the brightness and the color change value of the skin, and the result was shown in Table 4 below. The larger the value of brightness and color change, the better the skin tone.

Test substance Skin tone improvement rate (%) Brightness (mean ± standard deviation) Color (mean ± standard deviation) Formulation Example 1 12 ± 3.04 11 ± 1.89 Formulation Example 2 13 ± 2.95 13 ± 2.12 Formulation Example 3 15 ± 3.25 13 ± 2.03 Comparative Formulation Example 1 11 ± 2.02 10 ± 2.18 Comparative Formulation Example 2 10 ± 2.38 9 ± 2.16 Comparative Formulation Example 3 5 ± 2.34 5 ± 2.05

The results of Table 4 show that Comparative Formulation Example 3 containing no ginseng polysaccharide and green tea polysaccharide showed no significant skin tone improvement effect while Comparative Formulations 1 and 2 containing each of the green tea polysaccharides of the ginseng polysaccharide , The skin tone was improved after use compared to before use. In Formulations 1 to 3 containing both the polysaccharide of ginseng and the polysaccharide of green tea, a significantly higher skin tone improvement effect than Comparative Formulations 1 and 2 .

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Softening lotion (skin lotion)

The softening longevity was prepared by a conventional method according to the composition shown in Table 5 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Reference Example 1 0.1 The green tea polysaccharide of Reference Example 2 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 6 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Reference Example 1 0.5 The green tea polysaccharide of Reference Example 2 0.5 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Nutritive creams were prepared according to the compositions listed in Table 7 below in a conventional manner.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Reference Example 1 1.0 The green tea polysaccharide of Reference Example 2 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 8 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Reference Example 1 1.5 The green tea polysaccharide of Reference Example 2 1.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 9 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Reference Example 1 0.1 The green tea polysaccharide of Reference Example 2 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] Ointment

Ointments were prepared in a conventional manner according to the composition shown in Table 10 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Reference Example 1 0.1 The green tea polysaccharide of Reference Example 2 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A composition for external whitening skin comprising a ginseng polysaccharide and a green tea polysaccharide as an active ingredient. [2] The composition for external application for skin according to claim 1, wherein the ginseng polysaccharide and the green tea polysaccharide are contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. A pharmaceutical composition for skin whitening comprising ginseng polysaccharide and green tea polysaccharide as an active ingredient. [Claim 5] The pharmaceutical composition according to claim 4, wherein the ginseng polysaccharide and the green tea polysaccharide are contained in an amount of 0.001-10 wt% based on the total weight of the composition. [Claim 5] The pharmaceutical composition according to claim 4, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1.