KR20000070174A - 종양 괴사 인자 수용체 6α 및 6β - Google Patents

Info

Publication number

KR20000070174A

KR20000070174A KR1019997006400A KR19997006400A KR20000070174A KR 20000070174 A KR20000070174 A KR 20000070174A KR 1019997006400 A KR1019997006400 A KR 1019997006400A KR 19997006400 A KR19997006400 A KR 19997006400A KR 20000070174 A KR20000070174 A KR 20000070174A

Authority

KR

South Korea

Prior art keywords

seq

tnfr

amino acid

polypeptide

acid sequence

Prior art date

1997-01-14

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Ceased

Links

• Espacenet
• Global Dossier
• Discuss

• 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 title claims abstract description 256
• 102000003298 tumor necrosis factor receptor Human genes 0.000 title claims abstract description 251
• 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 296
• 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 283
• 229920001184 polypeptide Polymers 0.000 claims abstract description 271
• 238000000034 method Methods 0.000 claims abstract description 98
• 150000007523 nucleic acids Chemical class 0.000 claims abstract description 78
• 102000039446 nucleic acids Human genes 0.000 claims abstract description 69
• 108020004707 nucleic acids Proteins 0.000 claims abstract description 69
• 239000013598 vector Substances 0.000 claims abstract description 44
• 230000000694 effects Effects 0.000 claims abstract description 31
• 210000004027 cell Anatomy 0.000 claims description 144
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 131
• 239000002773 nucleotide Substances 0.000 claims description 85
• 125000003729 nucleotide group Chemical group 0.000 claims description 85
• 239000002299 complementary DNA Substances 0.000 claims description 65
• 108091033319 polynucleotide Proteins 0.000 claims description 61
• 102000040430 polynucleotide Human genes 0.000 claims description 61
• 239000002157 polynucleotide Substances 0.000 claims description 61
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 48
• 150000001413 amino acids Chemical class 0.000 claims description 46
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 39
• 239000000203 mixture Substances 0.000 claims description 25
• 238000012217 deletion Methods 0.000 claims description 21
• 230000037430 deletion Effects 0.000 claims description 21
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 14
• 238000004519 manufacturing process Methods 0.000 claims description 13
• 125000000539 amino acid group Chemical group 0.000 claims description 12
• 230000000295 complement effect Effects 0.000 claims description 9
• 238000009396 hybridization Methods 0.000 claims description 9
• 210000004899 c-terminal region Anatomy 0.000 claims description 8
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 6
• 238000002955 isolation Methods 0.000 claims description 2
• 238000012258 culturing Methods 0.000 claims 1
• 238000003306 harvesting Methods 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 abstract description 155
• 102000004169 proteins and genes Human genes 0.000 abstract description 107
• 201000010099 disease Diseases 0.000 abstract description 29
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 29
• 241000282414 Homo sapiens Species 0.000 abstract description 27
• 239000005557 antagonist Substances 0.000 abstract description 27
• 239000000556 agonist Substances 0.000 abstract description 22
• 238000012216 screening Methods 0.000 abstract description 16
• 210000000987 immune system Anatomy 0.000 abstract description 15
• 238000002405 diagnostic procedure Methods 0.000 abstract description 5
• 238000010188 recombinant method Methods 0.000 abstract description 5
• 238000002560 therapeutic procedure Methods 0.000 abstract description 3
• 235000018102 proteins Nutrition 0.000 description 105
• 239000003446 ligand Substances 0.000 description 56
• 102000005962 receptors Human genes 0.000 description 56
• 108020003175 receptors Proteins 0.000 description 56
• 230000014509 gene expression Effects 0.000 description 55
• 235000001014 amino acid Nutrition 0.000 description 54
• 239000012634 fragment Substances 0.000 description 51
• 108020004414 DNA Proteins 0.000 description 48
• 229940024606 amino acid Drugs 0.000 description 46
• 210000001519 tissue Anatomy 0.000 description 38
• 230000027455 binding Effects 0.000 description 35
• 238000009739 binding Methods 0.000 description 33
• 150000001875 compounds Chemical class 0.000 description 31
• 239000013612 plasmid Substances 0.000 description 27
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 23
• 108020004999 messenger RNA Proteins 0.000 description 23
• 239000013615 primer Substances 0.000 description 23
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 22
• 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 21
• 230000006907 apoptotic process Effects 0.000 description 19
• 230000035772 mutation Effects 0.000 description 19
• 241000588724 Escherichia coli Species 0.000 description 18
• 230000014616 translation Effects 0.000 description 18
• 230000036755 cellular response Effects 0.000 description 17
• 238000006467 substitution reaction Methods 0.000 description 17
• 238000013519 translation Methods 0.000 description 17
• 206010028980 Neoplasm Diseases 0.000 description 15
• 239000000047 product Substances 0.000 description 15
• 238000004458 analytical method Methods 0.000 description 14
• 230000000890 antigenic effect Effects 0.000 description 14
• 238000010367 cloning Methods 0.000 description 14
• 108020001507 fusion proteins Proteins 0.000 description 14
• 102000037865 fusion proteins Human genes 0.000 description 14
• 239000002609 medium Substances 0.000 description 14
• 238000003752 polymerase chain reaction Methods 0.000 description 14
• 238000000746 purification Methods 0.000 description 14
• 208000026278 immune system disease Diseases 0.000 description 13
• 108020004705 Codon Proteins 0.000 description 12
• 210000001744 T-lymphocyte Anatomy 0.000 description 12
• 241000700605 Viruses Species 0.000 description 12
• 238000003776 cleavage reaction Methods 0.000 description 12
• 230000007017 scission Effects 0.000 description 12
• 239000011780 sodium chloride Substances 0.000 description 12
• 239000000243 solution Substances 0.000 description 12
• 238000013518 transcription Methods 0.000 description 12
• 230000035897 transcription Effects 0.000 description 12
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 11
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 11
• 239000000427 antigen Substances 0.000 description 11
• 108091007433 antigens Proteins 0.000 description 11
• 102000036639 antigens Human genes 0.000 description 11
• 230000004071 biological effect Effects 0.000 description 11
• 210000001124 body fluid Anatomy 0.000 description 11
• 239000010839 body fluid Substances 0.000 description 11
• 210000000349 chromosome Anatomy 0.000 description 11
• 229960000485 methotrexate Drugs 0.000 description 11
• 239000000523 sample Substances 0.000 description 11
• 238000003556 assay Methods 0.000 description 10
• 239000012472 biological sample Substances 0.000 description 10
• 235000018417 cysteine Nutrition 0.000 description 10
• 230000008488 polyadenylation Effects 0.000 description 10
• 230000019491 signal transduction Effects 0.000 description 10
• 230000004913 activation Effects 0.000 description 9
• 238000003745 diagnosis Methods 0.000 description 9
• 239000013604 expression vector Substances 0.000 description 9
• 210000004408 hybridoma Anatomy 0.000 description 9
• 238000001727 in vivo Methods 0.000 description 9
• 210000002966 serum Anatomy 0.000 description 9
• 208000030507 AIDS Diseases 0.000 description 8
• 108090000542 Lymphotoxin-alpha Proteins 0.000 description 8
• 241001465754 Metazoa Species 0.000 description 8
• 230000002759 chromosomal effect Effects 0.000 description 8
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 8
• 238000005516 engineering process Methods 0.000 description 8
• 230000002163 immunogen Effects 0.000 description 8
• 108091008146 restriction endonucleases Proteins 0.000 description 8
• 238000012163 sequencing technique Methods 0.000 description 8
• 241000701447 unidentified baculovirus Species 0.000 description 8
• 102000004127 Cytokines Human genes 0.000 description 7
• 108090000695 Cytokines Proteins 0.000 description 7
• 241000124008 Mammalia Species 0.000 description 7
• 229960000723 ampicillin Drugs 0.000 description 7
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 7
• 201000011510 cancer Diseases 0.000 description 7
• 230000007423 decrease Effects 0.000 description 7
• 238000009472 formulation Methods 0.000 description 7
• 230000001404 mediated effect Effects 0.000 description 7
• 230000004048 modification Effects 0.000 description 7
• 238000012986 modification Methods 0.000 description 7
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 7
• 238000001890 transfection Methods 0.000 description 7
• 241000701022 Cytomegalovirus Species 0.000 description 6
• 241000238631 Hexapoda Species 0.000 description 6
• 101000611023 Homo sapiens Tumor necrosis factor receptor superfamily member 6 Proteins 0.000 description 6
• 108060003951 Immunoglobulin Proteins 0.000 description 6
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 6
• 206010052779 Transplant rejections Diseases 0.000 description 6
• 102100031988 Tumor necrosis factor ligand superfamily member 6 Human genes 0.000 description 6
• 102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 description 6
• 102100040403 Tumor necrosis factor receptor superfamily member 6 Human genes 0.000 description 6
• 238000007792 addition Methods 0.000 description 6
• 239000011543 agarose gel Substances 0.000 description 6
• 230000001580 bacterial effect Effects 0.000 description 6
• PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 6
• 238000003384 imaging method Methods 0.000 description 6
• 102000018358 immunoglobulin Human genes 0.000 description 6
• 238000002372 labelling Methods 0.000 description 6
• 210000004962 mammalian cell Anatomy 0.000 description 6
• 229930182817 methionine Natural products 0.000 description 6
• 230000035755 proliferation Effects 0.000 description 6
• 230000004044 response Effects 0.000 description 6
• 241000894006 Bacteria Species 0.000 description 5
• 101150013553 CD40 gene Proteins 0.000 description 5
• 108020004635 Complementary DNA Proteins 0.000 description 5
• 101150074155 DHFR gene Proteins 0.000 description 5
• 102000004190 Enzymes Human genes 0.000 description 5
• 108090000790 Enzymes Proteins 0.000 description 5
• 108010039471 Fas Ligand Protein Proteins 0.000 description 5
• 102100026238 Lymphotoxin-alpha Human genes 0.000 description 5
• 206010040070 Septic Shock Diseases 0.000 description 5
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 5
• 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 5
• 230000000692 anti-sense effect Effects 0.000 description 5
• 238000004113 cell culture Methods 0.000 description 5
• 238000005119 centrifugation Methods 0.000 description 5
• 230000008859 change Effects 0.000 description 5
• 239000003184 complementary RNA Substances 0.000 description 5
• 230000000875 corresponding effect Effects 0.000 description 5
• 230000002950 deficient Effects 0.000 description 5
• 210000002889 endothelial cell Anatomy 0.000 description 5
• 229940088598 enzyme Drugs 0.000 description 5
• 230000002068 genetic effect Effects 0.000 description 5
• 229960000789 guanidine hydrochloride Drugs 0.000 description 5
• 238000000338 in vitro Methods 0.000 description 5
• 208000015181 infectious disease Diseases 0.000 description 5
• 210000001672 ovary Anatomy 0.000 description 5
• 210000002381 plasma Anatomy 0.000 description 5
• 230000002829 reductive effect Effects 0.000 description 5
• 230000003248 secreting effect Effects 0.000 description 5
• 239000001632 sodium acetate Substances 0.000 description 5
• 235000017281 sodium acetate Nutrition 0.000 description 5
• 239000000126 substance Substances 0.000 description 5
• 239000006228 supernatant Substances 0.000 description 5
• 230000001225 therapeutic effect Effects 0.000 description 5
• 108020005544 Antisense RNA Proteins 0.000 description 4
• 208000023275 Autoimmune disease Diseases 0.000 description 4
• 102100023995 Beta-nerve growth factor Human genes 0.000 description 4
• 108091026890 Coding region Proteins 0.000 description 4
• 102000053602 DNA Human genes 0.000 description 4
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
• 101150064015 FAS gene Proteins 0.000 description 4
• 102100026693 FAS-associated death domain protein Human genes 0.000 description 4
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
• 208000031886 HIV Infections Diseases 0.000 description 4
• 208000037357 HIV infectious disease Diseases 0.000 description 4
• 101000851376 Homo sapiens Tumor necrosis factor receptor superfamily member 8 Proteins 0.000 description 4
• 241000699666 Mus <mouse, genus> Species 0.000 description 4
• 101100425758 Mus musculus Tnfrsf1b gene Proteins 0.000 description 4
• 238000005481 NMR spectroscopy Methods 0.000 description 4
• 108010025020 Nerve Growth Factor Proteins 0.000 description 4
• 108091034117 Oligonucleotide Proteins 0.000 description 4
• 108010038807 Oligopeptides Proteins 0.000 description 4
• 102000015636 Oligopeptides Human genes 0.000 description 4
• 108020004511 Recombinant DNA Proteins 0.000 description 4
• 108091058545 Secretory proteins Proteins 0.000 description 4
• 102000040739 Secretory proteins Human genes 0.000 description 4
• 238000012300 Sequence Analysis Methods 0.000 description 4
• 108091081024 Start codon Proteins 0.000 description 4
• 101150009046 Tnfrsf1a gene Proteins 0.000 description 4
• 239000007983 Tris buffer Substances 0.000 description 4
• 102100033725 Tumor necrosis factor receptor superfamily member 16 Human genes 0.000 description 4
• 101710187743 Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 description 4
• 102100036857 Tumor necrosis factor receptor superfamily member 8 Human genes 0.000 description 4
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
• 230000008827 biological function Effects 0.000 description 4
• 230000031018 biological processes and functions Effects 0.000 description 4
• 230000015572 biosynthetic process Effects 0.000 description 4
• 210000004978 chinese hamster ovary cell Anatomy 0.000 description 4
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 4
• 230000006378 damage Effects 0.000 description 4
• 210000003527 eukaryotic cell Anatomy 0.000 description 4
• 230000006870 function Effects 0.000 description 4
• 210000003917 human chromosome Anatomy 0.000 description 4
• 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 4
• 230000005764 inhibitory process Effects 0.000 description 4
• 238000003780 insertion Methods 0.000 description 4
• 230000037431 insertion Effects 0.000 description 4
• 230000003834 intracellular effect Effects 0.000 description 4
• 229930027917 kanamycin Natural products 0.000 description 4
• 229960000318 kanamycin Drugs 0.000 description 4
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
• 229930182823 kanamycin A Natural products 0.000 description 4
• 239000002502 liposome Substances 0.000 description 4
• 210000004698 lymphocyte Anatomy 0.000 description 4
• 244000005700 microbiome Species 0.000 description 4
• 229940053128 nerve growth factor Drugs 0.000 description 4
• 231100000252 nontoxic Toxicity 0.000 description 4
• 230000003000 nontoxic effect Effects 0.000 description 4
• 239000008194 pharmaceutical composition Substances 0.000 description 4
• 238000002360 preparation method Methods 0.000 description 4
• 210000002307 prostate Anatomy 0.000 description 4
• 238000007423 screening assay Methods 0.000 description 4
• 238000013268 sustained release Methods 0.000 description 4
• 239000012730 sustained-release form Substances 0.000 description 4
• 241001430294 unidentified retrovirus Species 0.000 description 4
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
• 238000001086 yeast two-hybrid system Methods 0.000 description 4
• MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 3
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
• 108020004491 Antisense DNA Proteins 0.000 description 3
• 102100027207 CD27 antigen Human genes 0.000 description 3
• 241000699802 Cricetulus griseus Species 0.000 description 3
• 239000003155 DNA primer Substances 0.000 description 3
• 238000001712 DNA sequencing Methods 0.000 description 3
• 102000010170 Death domains Human genes 0.000 description 3
• 108050001718 Death domains Proteins 0.000 description 3
• 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 3
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
• HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 3
• 101710154606 Hemagglutinin Proteins 0.000 description 3
• 101000914511 Homo sapiens CD27 antigen Proteins 0.000 description 3
• 101000911074 Homo sapiens FAS-associated death domain protein Proteins 0.000 description 3
• 101000851370 Homo sapiens Tumor necrosis factor receptor superfamily member 9 Proteins 0.000 description 3
• 206010061598 Immunodeficiency Diseases 0.000 description 3
• 208000029462 Immunodeficiency disease Diseases 0.000 description 3
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 3
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 3
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 3
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
• 102000004083 Lymphotoxin-alpha Human genes 0.000 description 3
• 229930193140 Neomycin Natural products 0.000 description 3
• 108010032605 Nerve Growth Factor Receptors Proteins 0.000 description 3
• 238000000636 Northern blotting Methods 0.000 description 3
• 101710093908 Outer capsid protein VP4 Proteins 0.000 description 3
• 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 3
• 206010035226 Plasma cell myeloma Diseases 0.000 description 3
• 101710176177 Protein A56 Proteins 0.000 description 3
• 108091028664 Ribonucleotide Proteins 0.000 description 3
• 101710187830 Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 description 3
• 102100033733 Tumor necrosis factor receptor superfamily member 1B Human genes 0.000 description 3
• 101710165473 Tumor necrosis factor receptor superfamily member 4 Proteins 0.000 description 3
• 102100022153 Tumor necrosis factor receptor superfamily member 4 Human genes 0.000 description 3
• 102100036856 Tumor necrosis factor receptor superfamily member 9 Human genes 0.000 description 3
• 230000002159 abnormal effect Effects 0.000 description 3
• -1 aliphatic amino acids Chemical class 0.000 description 3
• 230000003321 amplification Effects 0.000 description 3
• 150000001450 anions Chemical class 0.000 description 3
• 239000003816 antisense DNA Substances 0.000 description 3
• 239000000872 buffer Substances 0.000 description 3
• 238000004364 calculation method Methods 0.000 description 3
• 230000001413 cellular effect Effects 0.000 description 3
• 238000012761 co-transfection Methods 0.000 description 3
• 238000004590 computer program Methods 0.000 description 3
• 230000003013 cytotoxicity Effects 0.000 description 3
• 231100000135 cytotoxicity Toxicity 0.000 description 3
• 238000001514 detection method Methods 0.000 description 3
• 238000011161 development Methods 0.000 description 3
• 239000003937 drug carrier Substances 0.000 description 3
• 239000012636 effector Substances 0.000 description 3
• 239000002158 endotoxin Substances 0.000 description 3
• 239000003623 enhancer Substances 0.000 description 3
• 102000018823 fas Receptor Human genes 0.000 description 3
• 238000002509 fluorescent in situ hybridization Methods 0.000 description 3
• 230000004927 fusion Effects 0.000 description 3
• 229960002989 glutamic acid Drugs 0.000 description 3
• 230000009931 harmful effect Effects 0.000 description 3
• 230000002209 hydrophobic effect Effects 0.000 description 3
• 230000002519 immonomodulatory effect Effects 0.000 description 3
• 230000007813 immunodeficiency Effects 0.000 description 3
• 230000001939 inductive effect Effects 0.000 description 3
• 238000001802 infusion Methods 0.000 description 3
• 238000001990 intravenous administration Methods 0.000 description 3
• BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 3
• 210000002510 keratinocyte Anatomy 0.000 description 3
• 230000002147 killing effect Effects 0.000 description 3
• 238000013507 mapping Methods 0.000 description 3
• 239000003550 marker Substances 0.000 description 3
• 239000000463 material Substances 0.000 description 3
• 239000012528 membrane Substances 0.000 description 3
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 3
• 201000000050 myeloid neoplasm Diseases 0.000 description 3
• 229960004927 neomycin Drugs 0.000 description 3
• 230000004770 neurodegeneration Effects 0.000 description 3
• 208000015122 neurodegenerative disease Diseases 0.000 description 3
• 238000003199 nucleic acid amplification method Methods 0.000 description 3
• 238000012545 processing Methods 0.000 description 3
• 230000004952 protein activity Effects 0.000 description 3
• 230000010076 replication Effects 0.000 description 3
• 230000001177 retroviral effect Effects 0.000 description 3
• 239000002336 ribonucleotide Substances 0.000 description 3
• 125000002652 ribonucleotide group Chemical group 0.000 description 3
• 230000028327 secretion Effects 0.000 description 3
• 230000035939 shock Effects 0.000 description 3
• 230000011664 signaling Effects 0.000 description 3
• 241000894007 species Species 0.000 description 3
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
• 210000002700 urine Anatomy 0.000 description 3
• 239000003981 vehicle Substances 0.000 description 3
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
• NKDFYOWSKOHCCO-YPVLXUMRSA-N 20-hydroxyecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@](C)(O)[C@H](O)CCC(C)(O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 NKDFYOWSKOHCCO-YPVLXUMRSA-N 0.000 description 2
• 239000004475 Arginine Substances 0.000 description 2
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
• 241000201370 Autographa californica nucleopolyhedrovirus Species 0.000 description 2
• 125000001433 C-terminal amino-acid group Chemical group 0.000 description 2
• 108010029697 CD40 Ligand Proteins 0.000 description 2
• 102100032937 CD40 ligand Human genes 0.000 description 2
• 206010006895 Cachexia Diseases 0.000 description 2
• 101100230428 Caenorhabditis elegans hil-5 gene Proteins 0.000 description 2
• 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 2
• 108091033380 Coding strand Proteins 0.000 description 2
• 208000035473 Communicable disease Diseases 0.000 description 2
• WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
• 102000012410 DNA Ligases Human genes 0.000 description 2
• 108010061982 DNA Ligases Proteins 0.000 description 2
• 229920002307 Dextran Polymers 0.000 description 2
• 102100024746 Dihydrofolate reductase Human genes 0.000 description 2
• 206010061819 Disease recurrence Diseases 0.000 description 2
• AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 2
• 206010058314 Dysplasia Diseases 0.000 description 2
• 238000002965 ELISA Methods 0.000 description 2
• 241000196324 Embryophyta Species 0.000 description 2
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
• 241001131785 Escherichia coli HB101 Species 0.000 description 2
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
• 239000004471 Glycine Substances 0.000 description 2
• 108010093488 His-His-His-His-His-His Proteins 0.000 description 2
• 241000282412 Homo Species 0.000 description 2
• 101000850748 Homo sapiens Tumor necrosis factor receptor type 1-associated DEATH domain protein Proteins 0.000 description 2
• 206010020751 Hypersensitivity Diseases 0.000 description 2
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
• 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 2
• 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 2
• 108091092195 Intron Proteins 0.000 description 2
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
• 102100026894 Lymphotoxin-beta Human genes 0.000 description 2
• 108090000362 Lymphotoxin-beta Proteins 0.000 description 2
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
• 241000699670 Mus sp. Species 0.000 description 2
• 102000003945 NF-kappa B Human genes 0.000 description 2
• 108010057466 NF-kappa B Proteins 0.000 description 2
• 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
• 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
• 101710182846 Polyhedrin Proteins 0.000 description 2
• 239000004365 Protease Substances 0.000 description 2
• 108010076504 Protein Sorting Signals Proteins 0.000 description 2
• 108091034057 RNA (poly(A)) Proteins 0.000 description 2
• 241000700159 Rattus Species 0.000 description 2
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
• 108091081062 Repeated sequence (DNA) Proteins 0.000 description 2
• 108020005091 Replication Origin Proteins 0.000 description 2
• 206010039491 Sarcoma Diseases 0.000 description 2
• 230000006052 T cell proliferation Effects 0.000 description 2
• 108010022394 Threonine synthase Proteins 0.000 description 2
• 102100033081 Tumor necrosis factor receptor type 1-associated DEATH domain protein Human genes 0.000 description 2
• DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Chemical compound CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
• HMNZFMSWFCAGGW-XPWSMXQVSA-N [3-[hydroxy(2-hydroxyethoxy)phosphoryl]oxy-2-[(e)-octadec-9-enoyl]oxypropyl] (e)-octadec-9-enoate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(=O)OCCO)OC(=O)CCCCCCC\C=C\CCCCCCCC HMNZFMSWFCAGGW-XPWSMXQVSA-N 0.000 description 2
• 239000002253 acid Substances 0.000 description 2
• 238000001042 affinity chromatography Methods 0.000 description 2
• 230000002776 aggregation Effects 0.000 description 2
• 238000004220 aggregation Methods 0.000 description 2
• 235000004279 alanine Nutrition 0.000 description 2
• 238000010171 animal model Methods 0.000 description 2
• 230000000840 anti-viral effect Effects 0.000 description 2
• 230000005875 antibody response Effects 0.000 description 2
• 238000003782 apoptosis assay Methods 0.000 description 2
• 239000007864 aqueous solution Substances 0.000 description 2
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
• 235000009697 arginine Nutrition 0.000 description 2
• 235000003704 aspartic acid Nutrition 0.000 description 2
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
• 230000033228 biological regulation Effects 0.000 description 2
• 210000004369 blood Anatomy 0.000 description 2
• 239000008280 blood Substances 0.000 description 2
• 239000001506 calcium phosphate Substances 0.000 description 2
• 229910000389 calcium phosphate Inorganic materials 0.000 description 2
• 235000011010 calcium phosphates Nutrition 0.000 description 2
• 238000004422 calculation algorithm Methods 0.000 description 2
• 244000309466 calf Species 0.000 description 2
• 229910052799 carbon Inorganic materials 0.000 description 2
• 230000004663 cell proliferation Effects 0.000 description 2
• HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
• 238000004587 chromatography analysis Methods 0.000 description 2
• 230000001684 chronic effect Effects 0.000 description 2
• 238000012790 confirmation Methods 0.000 description 2
• 238000010276 construction Methods 0.000 description 2
• 239000000356 contaminant Substances 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• 230000002596 correlated effect Effects 0.000 description 2
• 150000001945 cysteines Chemical group 0.000 description 2
• 239000005547 deoxyribonucleotide Substances 0.000 description 2
• 230000001419 dependent effect Effects 0.000 description 2
• 229960002086 dextran Drugs 0.000 description 2
• 102000004419 dihydrofolate reductase Human genes 0.000 description 2
• 108020001096 dihydrofolate reductase Proteins 0.000 description 2
• 238000010790 dilution Methods 0.000 description 2
• 239000012895 dilution Substances 0.000 description 2
• VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 2
• 239000003814 drug Substances 0.000 description 2
• 239000013613 expression plasmid Substances 0.000 description 2
• 238000000605 extraction Methods 0.000 description 2
• 108010052621 fas Receptor Proteins 0.000 description 2
• 238000001914 filtration Methods 0.000 description 2
• 235000019688 fish Nutrition 0.000 description 2
• 239000012530 fluid Substances 0.000 description 2
• 238000007429 general method Methods 0.000 description 2
• 235000013922 glutamic acid Nutrition 0.000 description 2
• 239000004220 glutamic acid Substances 0.000 description 2
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
• 239000001963 growth medium Substances 0.000 description 2
• 238000004128 high performance liquid chromatography Methods 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 239000005556 hormone Substances 0.000 description 2
• 229940088597 hormone Drugs 0.000 description 2
• 230000003053 immunization Effects 0.000 description 2
• 238000002649 immunization Methods 0.000 description 2
• 229940072221 immunoglobulins Drugs 0.000 description 2
• 238000007901 in situ hybridization Methods 0.000 description 2
• 210000003000 inclusion body Anatomy 0.000 description 2
• 238000011534 incubation Methods 0.000 description 2
• 206010022000 influenza Diseases 0.000 description 2
• 239000003112 inhibitor Substances 0.000 description 2
• 230000002401 inhibitory effect Effects 0.000 description 2
• 230000003993 interaction Effects 0.000 description 2
• 108020001756 ligand binding domains Proteins 0.000 description 2
• 230000000670 limiting effect Effects 0.000 description 2
• 150000002632 lipids Chemical class 0.000 description 2
• 239000007788 liquid Substances 0.000 description 2
• 208000019423 liver disease Diseases 0.000 description 2
• 210000002752 melanocyte Anatomy 0.000 description 2
• 230000000813 microbial effect Effects 0.000 description 2
• 230000000394 mitotic effect Effects 0.000 description 2
• 238000002156 mixing Methods 0.000 description 2
• 238000010369 molecular cloning Methods 0.000 description 2
• 210000001616 monocyte Anatomy 0.000 description 2
• 239000000178 monomer Substances 0.000 description 2
• 230000004660 morphological change Effects 0.000 description 2
• 230000006654 negative regulation of apoptotic process Effects 0.000 description 2
• 230000007935 neutral effect Effects 0.000 description 2
• 230000002018 overexpression Effects 0.000 description 2
• 239000007800 oxidant agent Substances 0.000 description 2
• 238000004806 packaging method and process Methods 0.000 description 2
• 239000008188 pellet Substances 0.000 description 2
• 230000006461 physiological response Effects 0.000 description 2
• 229920001223 polyethylene glycol Polymers 0.000 description 2
• 230000003389 potentiating effect Effects 0.000 description 2
• 239000002244 precipitate Substances 0.000 description 2
• 108010066381 preproinsulin Proteins 0.000 description 2
• 239000002987 primer (paints) Substances 0.000 description 2
• 230000005522 programmed cell death Effects 0.000 description 2
• 210000001236 prokaryotic cell Anatomy 0.000 description 2
• 238000003127 radioimmunoassay Methods 0.000 description 2
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
• 238000011160 research Methods 0.000 description 2
• 150000003839 salts Chemical class 0.000 description 2
• 239000006152 selective media Substances 0.000 description 2
• 230000036303 septic shock Effects 0.000 description 2
• 239000011734 sodium Substances 0.000 description 2
• 229910052708 sodium Inorganic materials 0.000 description 2
• 239000007787 solid Substances 0.000 description 2
• 210000004989 spleen cell Anatomy 0.000 description 2
• 238000010561 standard procedure Methods 0.000 description 2
• 238000003860 storage Methods 0.000 description 2
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
• 238000007920 subcutaneous administration Methods 0.000 description 2
• 239000000725 suspension Substances 0.000 description 2
• 208000011580 syndromic disease Diseases 0.000 description 2
• 210000001179 synovial fluid Anatomy 0.000 description 2
• 239000003053 toxin Substances 0.000 description 2
• 231100000765 toxin Toxicity 0.000 description 2
• 108700012359 toxins Proteins 0.000 description 2
• 230000005030 transcription termination Effects 0.000 description 2
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
• 241000701161 unidentified adenovirus Species 0.000 description 2
• 241001529453 unidentified herpesvirus Species 0.000 description 2
• 230000003612 virological effect Effects 0.000 description 2
• 238000005406 washing Methods 0.000 description 2
• XMQUEQJCYRFIQS-YFKPBYRVSA-N (2s)-2-amino-5-ethoxy-5-oxopentanoic acid Chemical compound CCOC(=O)CC[C@H](N)C(O)=O XMQUEQJCYRFIQS-YFKPBYRVSA-N 0.000 description 1
• 125000006701 (C1-C7) alkyl group Chemical group 0.000 description 1
• NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
• IQFYYKKMVGJFEH-OFKYTIFKSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(tritiooxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](CO[3H])O[C@H]1N1C(=O)NC(=O)C(C)=C1 IQFYYKKMVGJFEH-OFKYTIFKSA-N 0.000 description 1
• ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical group CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
• 108010082808 4-1BB Ligand Proteins 0.000 description 1
• QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
• ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
• 208000029483 Acquired immunodeficiency Diseases 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 241000701386 African swine fever virus Species 0.000 description 1
• 101100216424 African swine fever virus (strain E-70 MS44) LMW5-HL gene Proteins 0.000 description 1
• 229920001817 Agar Polymers 0.000 description 1
• 108010088751 Albumins Proteins 0.000 description 1
• 102000009027 Albumins Human genes 0.000 description 1
• 108700028369 Alleles Proteins 0.000 description 1
• 208000024827 Alzheimer disease Diseases 0.000 description 1
• 108010032595 Antibody Binding Sites Proteins 0.000 description 1
• 208000032467 Aplastic anaemia Diseases 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 241000972773 Aulopiformes Species 0.000 description 1
• 238000012935 Averaging Methods 0.000 description 1
• 230000003844 B-cell-activation Effects 0.000 description 1
• 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 description 1
• 108700032588 Baculovirus p35 Proteins 0.000 description 1
• DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
• 108010006654 Bleomycin Proteins 0.000 description 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
• 108010046080 CD27 Ligand Proteins 0.000 description 1
• 102100025221 CD70 antigen Human genes 0.000 description 1
• 101150004850 CRMB gene Proteins 0.000 description 1
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
• 102000014914 Carrier Proteins Human genes 0.000 description 1
• 108010078791 Carrier Proteins Proteins 0.000 description 1
• 102000004091 Caspase-8 Human genes 0.000 description 1
• 108090000538 Caspase-8 Proteins 0.000 description 1
• 102000000844 Cell Surface Receptors Human genes 0.000 description 1
• 108010001857 Cell Surface Receptors Proteins 0.000 description 1
• 241000282693 Cercopithecidae Species 0.000 description 1
• 206010063094 Cerebral malaria Diseases 0.000 description 1
• KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
• 102100038385 Coiled-coil domain-containing protein R3HCC1L Human genes 0.000 description 1
• 108020004394 Complementary RNA Proteins 0.000 description 1
• UHDGCWIWMRVCDJ-CCXZUQQUSA-N Cytarabine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O1 UHDGCWIWMRVCDJ-CCXZUQQUSA-N 0.000 description 1
• FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
• FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
• FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
• YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
• 239000004375 Dextrin Substances 0.000 description 1
• 229920001353 Dextrin Polymers 0.000 description 1
• BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
• 241000255925 Diptera Species 0.000 description 1
• LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
• 108010042407 Endonucleases Proteins 0.000 description 1
• 102000004533 Endonucleases Human genes 0.000 description 1
• 101900034680 Epstein-Barr virus Apoptosis regulator BHRF1 Proteins 0.000 description 1
• 241001596967 Escherichia coli M15 Species 0.000 description 1
• 241000701959 Escherichia virus Lambda Species 0.000 description 1
• 108010077716 Fas-Associated Death Domain Protein Proteins 0.000 description 1
• 108010010803 Gelatin Proteins 0.000 description 1
• 206010064571 Gene mutation Diseases 0.000 description 1
• 206010018364 Glomerulonephritis Diseases 0.000 description 1
• 239000004366 Glucose oxidase Substances 0.000 description 1
• 108010015776 Glucose oxidase Proteins 0.000 description 1
• 230000010556 Heparin Binding Activity Effects 0.000 description 1
• 101000756632 Homo sapiens Actin, cytoplasmic 1 Proteins 0.000 description 1
• 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 description 1
• 101000743767 Homo sapiens Coiled-coil domain-containing protein R3HCC1L Proteins 0.000 description 1
• 101100369992 Homo sapiens TNFSF10 gene Proteins 0.000 description 1
• 101100207070 Homo sapiens TNFSF8 gene Proteins 0.000 description 1
• 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
• 102000002265 Human Growth Hormone Human genes 0.000 description 1
• 108010000521 Human Growth Hormone Proteins 0.000 description 1
• 239000000854 Human Growth Hormone Substances 0.000 description 1
• 108090000144 Human Proteins Proteins 0.000 description 1
• 102000003839 Human Proteins Human genes 0.000 description 1
• 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
• 108700002232 Immediate-Early Genes Proteins 0.000 description 1
• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
• 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 208000022559 Inflammatory bowel disease Diseases 0.000 description 1
• 108020005350 Initiator Codon Proteins 0.000 description 1
• 102000008070 Interferon-gamma Human genes 0.000 description 1
• 108010074328 Interferon-gamma Proteins 0.000 description 1
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
• 238000011050 LAL assay Methods 0.000 description 1
• 108010054278 Lac Repressors Proteins 0.000 description 1
• 101710192602 Latent membrane protein 1 Proteins 0.000 description 1
• 108090001090 Lectins Proteins 0.000 description 1
• 102000004856 Lectins Human genes 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• 239000004472 Lysine Substances 0.000 description 1
• 229930195725 Mannitol Natural products 0.000 description 1
• 101100278853 Mus musculus Dhfr gene Proteins 0.000 description 1
• 101100537545 Mus musculus Fas gene Proteins 0.000 description 1
• 101100153533 Mus musculus Ltbr gene Proteins 0.000 description 1
• 101100207071 Mus musculus Tnfsf8 gene Proteins 0.000 description 1
• 108010021466 Mutant Proteins Proteins 0.000 description 1
• 102000008300 Mutant Proteins Human genes 0.000 description 1
• HOKKHZGPKSLGJE-GSVOUGTGSA-N N-Methyl-D-aspartic acid Chemical compound CN[C@@H](C(O)=O)CC(O)=O HOKKHZGPKSLGJE-GSVOUGTGSA-N 0.000 description 1
• 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
• 125000000729 N-terminal amino-acid group Chemical group 0.000 description 1
• 108091008604 NGF receptors Proteins 0.000 description 1
• 206010028851 Necrosis Diseases 0.000 description 1
• 241001045988 Neogene Species 0.000 description 1
• 101100007739 Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) crmA gene Proteins 0.000 description 1
• 101710163270 Nuclease Proteins 0.000 description 1
• 108010027206 Nucleopolyhedrovirus inhibitor of apoptosis Proteins 0.000 description 1
• 108010042215 OX40 Ligand Proteins 0.000 description 1
• 102000004473 OX40 Ligand Human genes 0.000 description 1
• 108700026244 Open Reading Frames Proteins 0.000 description 1
• 241000283973 Oryctolagus cuniculus Species 0.000 description 1
• 206010061535 Ovarian neoplasm Diseases 0.000 description 1
• 229910019142 PO4 Inorganic materials 0.000 description 1
• 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
• 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
• 108090000526 Papain Proteins 0.000 description 1
• 208000018737 Parkinson disease Diseases 0.000 description 1
• 229930182555 Penicillin Natural products 0.000 description 1
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
• 102000057297 Pepsin A Human genes 0.000 description 1
• 108090000284 Pepsin A Proteins 0.000 description 1
• 108091005804 Peptidases Proteins 0.000 description 1
• 102000035195 Peptidases Human genes 0.000 description 1
• 108010033276 Peptide Fragments Proteins 0.000 description 1
• 102000007079 Peptide Fragments Human genes 0.000 description 1
• 102000011420 Phospholipase D Human genes 0.000 description 1
• 108090000553 Phospholipase D Proteins 0.000 description 1
• 102000015439 Phospholipases Human genes 0.000 description 1
• 108010064785 Phospholipases Proteins 0.000 description 1
• RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
• 239000002202 Polyethylene glycol Substances 0.000 description 1
• 206010060862 Prostate cancer Diseases 0.000 description 1
• 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
• 101100084022 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lapA gene Proteins 0.000 description 1
• 101800001295 Putative ATP-dependent helicase Proteins 0.000 description 1
• 101800001006 Putative helicase Proteins 0.000 description 1
• 239000012083 RIPA buffer Substances 0.000 description 1
• 102100022419 RPA-interacting protein Human genes 0.000 description 1
• 206010063837 Reperfusion injury Diseases 0.000 description 1
• 208000007014 Retinitis pigmentosa Diseases 0.000 description 1
• 102000006382 Ribonucleases Human genes 0.000 description 1
• 108010083644 Ribonucleases Proteins 0.000 description 1
• 108090000829 Ribosome Inactivating Proteins Proteins 0.000 description 1
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
• 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
• 108091081021 Sense strand Proteins 0.000 description 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
• 108010071390 Serum Albumin Proteins 0.000 description 1
• 102000007562 Serum Albumin Human genes 0.000 description 1
• 108020004682 Single-Stranded DNA Proteins 0.000 description 1
• 241000256248 Spodoptera Species 0.000 description 1
• 241000187747 Streptomyces Species 0.000 description 1
• NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
• 108090000925 TNF receptor-associated factor 2 Proteins 0.000 description 1
• 102000046283 TNF-Related Apoptosis-Inducing Ligand Human genes 0.000 description 1
• 108700012411 TNFSF10 Proteins 0.000 description 1
• 102100034779 TRAF family member-associated NF-kappa-B activator Human genes 0.000 description 1
• 239000004098 Tetracycline Substances 0.000 description 1
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
• 239000004473 Threonine Substances 0.000 description 1
• IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Natural products O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
• 108700009124 Transcription Initiation Site Proteins 0.000 description 1
• 108010009583 Transforming Growth Factors Proteins 0.000 description 1
• 102000009618 Transforming Growth Factors Human genes 0.000 description 1
• YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
• 102000004142 Trypsin Human genes 0.000 description 1
• 108090000631 Trypsin Proteins 0.000 description 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
• 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
• 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
• 108050002568 Tumor necrosis factor ligand superfamily member 6 Proteins 0.000 description 1
• 102100032100 Tumor necrosis factor ligand superfamily member 8 Human genes 0.000 description 1
• 102100032101 Tumor necrosis factor ligand superfamily member 9 Human genes 0.000 description 1
• 206010054094 Tumour necrosis Diseases 0.000 description 1
• 102000014384 Type C Phospholipases Human genes 0.000 description 1
• 108010079194 Type C Phospholipases Proteins 0.000 description 1
• 241000700618 Vaccinia virus Species 0.000 description 1
• 108020005202 Viral DNA Proteins 0.000 description 1
• 108700005077 Viral Genes Proteins 0.000 description 1
• 208000036142 Viral infection Diseases 0.000 description 1
• 241000269370 Xenopus <genus> Species 0.000 description 1
• 230000007488 abnormal function Effects 0.000 description 1
• 238000002835 absorbance Methods 0.000 description 1
• 239000008351 acetate buffer Substances 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 230000001154 acute effect Effects 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 239000008272 agar Substances 0.000 description 1
• 238000012867 alanine scanning Methods 0.000 description 1
• 208000026935 allergic disease Diseases 0.000 description 1
• 230000007815 allergy Effects 0.000 description 1
• 150000001408 amides Chemical group 0.000 description 1
• BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
• 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
• 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
• 235000011130 ammonium sulphate Nutrition 0.000 description 1
• 206010002026 amyotrophic lateral sclerosis Diseases 0.000 description 1
• 239000003098 androgen Substances 0.000 description 1
• 229940030486 androgens Drugs 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 238000005571 anion exchange chromatography Methods 0.000 description 1
• 208000022531 anorexia Diseases 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 230000001399 anti-metabolic effect Effects 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• 230000009830 antibody antigen interaction Effects 0.000 description 1
• 239000002246 antineoplastic agent Substances 0.000 description 1
• 208000034615 apoptosis-related disease Diseases 0.000 description 1
• 230000036528 appetite Effects 0.000 description 1
• 235000019789 appetite Nutrition 0.000 description 1
• 125000003118 aryl group Chemical group 0.000 description 1
• 229960005070 ascorbic acid Drugs 0.000 description 1
• 235000010323 ascorbic acid Nutrition 0.000 description 1
• 239000011668 ascorbic acid Substances 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 238000012098 association analyses Methods 0.000 description 1
• 210000003719 b-lymphocyte Anatomy 0.000 description 1
• 229910052788 barium Inorganic materials 0.000 description 1
• DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
• 230000008901 benefit Effects 0.000 description 1
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
• 108010005774 beta-Galactosidase Proteins 0.000 description 1
• IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
• DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
• 238000004166 bioassay Methods 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 229960000074 biopharmaceutical Drugs 0.000 description 1
• 229920001222 biopolymer Polymers 0.000 description 1
• 229960002685 biotin Drugs 0.000 description 1
• 235000020958 biotin Nutrition 0.000 description 1
• 239000011616 biotin Substances 0.000 description 1
• 229960001561 bleomycin Drugs 0.000 description 1
• OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
• 230000037396 body weight Effects 0.000 description 1
• 210000000481 breast Anatomy 0.000 description 1
• 239000007853 buffer solution Substances 0.000 description 1
• DQXBYHZEEUGOBF-UHFFFAOYSA-N but-3-enoic acid;ethene Chemical compound C=C.OC(=O)CC=C DQXBYHZEEUGOBF-UHFFFAOYSA-N 0.000 description 1
• 238000010804 cDNA synthesis Methods 0.000 description 1
• 229910052792 caesium Inorganic materials 0.000 description 1
• TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
• 239000011575 calcium Substances 0.000 description 1
• 229910052791 calcium Inorganic materials 0.000 description 1
• 150000001720 carbohydrates Chemical class 0.000 description 1
• 235000014633 carbohydrates Nutrition 0.000 description 1
• 239000000969 carrier Substances 0.000 description 1
• 238000005277 cation exchange chromatography Methods 0.000 description 1
• 239000003729 cation exchange resin Substances 0.000 description 1
• 101150055276 ced-3 gene Proteins 0.000 description 1
• 230000030833 cell death Effects 0.000 description 1
• 230000003915 cell function Effects 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 208000025434 cerebellar degeneration Diseases 0.000 description 1
• 239000002738 chelating agent Substances 0.000 description 1
• 239000003795 chemical substances by application Substances 0.000 description 1
• 230000000973 chemotherapeutic effect Effects 0.000 description 1
• 235000012000 cholesterol Nutrition 0.000 description 1
• 238000003200 chromosome mapping Methods 0.000 description 1
• DQLATGHUWYMOKM-UHFFFAOYSA-L cisplatin Chemical compound N[Pt](N)(Cl)Cl DQLATGHUWYMOKM-UHFFFAOYSA-L 0.000 description 1
• 229960004316 cisplatin Drugs 0.000 description 1
• 239000011248 coating agent Substances 0.000 description 1
• 239000008199 coating composition Substances 0.000 description 1
• 230000000052 comparative effect Effects 0.000 description 1
• 238000011109 contamination Methods 0.000 description 1
• 229920001577 copolymer Polymers 0.000 description 1
• 230000008878 coupling Effects 0.000 description 1
• 238000010168 coupling process Methods 0.000 description 1
• 238000005859 coupling reaction Methods 0.000 description 1
• 238000002425 crystallisation Methods 0.000 description 1
• 230000008025 crystallization Effects 0.000 description 1
• 239000002852 cysteine proteinase inhibitor Substances 0.000 description 1
• 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
• 229940127089 cytotoxic agent Drugs 0.000 description 1
• 230000034994 death Effects 0.000 description 1
• 206010061428 decreased appetite Diseases 0.000 description 1
• 230000003247 decreasing effect Effects 0.000 description 1
• 230000005860 defense response to virus Effects 0.000 description 1
• 230000007850 degeneration Effects 0.000 description 1
• 230000003412 degenerative effect Effects 0.000 description 1
• 238000004925 denaturation Methods 0.000 description 1
• 230000036425 denaturation Effects 0.000 description 1
• 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
• 230000030609 dephosphorylation Effects 0.000 description 1
• 238000006209 dephosphorylation reaction Methods 0.000 description 1
• 238000013461 design Methods 0.000 description 1
• 239000003599 detergent Substances 0.000 description 1
• 229910052805 deuterium Inorganic materials 0.000 description 1
• 229960000633 dextran sulfate Drugs 0.000 description 1
• 235000019425 dextrin Nutrition 0.000 description 1
• 206010012601 diabetes mellitus Diseases 0.000 description 1
• 238000010586 diagram Methods 0.000 description 1
• 238000000502 dialysis Methods 0.000 description 1
• 235000005911 diet Nutrition 0.000 description 1
• 230000037213 diet Effects 0.000 description 1
• OZRNSSUDZOLUSN-LBPRGKRZSA-N dihydrofolic acid Chemical compound N=1C=2C(=O)NC(N)=NC=2NCC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OZRNSSUDZOLUSN-LBPRGKRZSA-N 0.000 description 1
• 239000012470 diluted sample Substances 0.000 description 1
• 239000003085 diluting agent Substances 0.000 description 1
• 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
• 150000002016 disaccharides Chemical class 0.000 description 1
• 238000009826 distribution Methods 0.000 description 1
• 229960003638 dopamine Drugs 0.000 description 1
• 229960004679 doxorubicin Drugs 0.000 description 1
• 239000006196 drop Substances 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 230000004064 dysfunction Effects 0.000 description 1
• 230000001516 effect on protein Effects 0.000 description 1
• 238000000804 electron spin resonance spectroscopy Methods 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 210000002308 embryonic cell Anatomy 0.000 description 1
• 239000003995 emulsifying agent Substances 0.000 description 1
• 230000002708 enhancing effect Effects 0.000 description 1
• 238000009585 enzyme analysis Methods 0.000 description 1
• 239000003797 essential amino acid Substances 0.000 description 1
• 235000020776 essential amino acid Nutrition 0.000 description 1
• 229940011871 estrogen Drugs 0.000 description 1
• 239000000262 estrogen Substances 0.000 description 1
• 238000012869 ethanol precipitation Methods 0.000 description 1
• LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
• 229940093471 ethyl oleate Drugs 0.000 description 1
• 239000005038 ethylene vinyl acetate Substances 0.000 description 1
• 230000010429 evolutionary process Effects 0.000 description 1
• 230000029142 excretion Effects 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 238000000855 fermentation Methods 0.000 description 1
• 230000004151 fermentation Effects 0.000 description 1
• 239000012894 fetal calf serum Substances 0.000 description 1
• 230000001605 fetal effect Effects 0.000 description 1
• 239000000945 filler Substances 0.000 description 1
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
• 201000003444 follicular lymphoma Diseases 0.000 description 1
• 230000037433 frameshift Effects 0.000 description 1
• 230000002538 fungal effect Effects 0.000 description 1
• 239000000499 gel Substances 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 239000008273 gelatin Substances 0.000 description 1
• 229920000159 gelatin Polymers 0.000 description 1
• 235000019322 gelatine Nutrition 0.000 description 1
• 235000011852 gelatine desserts Nutrition 0.000 description 1
• 238000002523 gelfiltration Methods 0.000 description 1
• 238000010353 genetic engineering Methods 0.000 description 1
• 108060003196 globin Proteins 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 229940116332 glucose oxidase Drugs 0.000 description 1
• 235000019420 glucose oxidase Nutrition 0.000 description 1
• 229930195712 glutamate Natural products 0.000 description 1
• 229940049906 glutamate Drugs 0.000 description 1
• 239000006451 grace's insect medium Substances 0.000 description 1
• 230000009036 growth inhibition Effects 0.000 description 1
• 229960004198 guanidine Drugs 0.000 description 1
• 229940093915 gynecological organic acid Drugs 0.000 description 1
• 230000003394 haemopoietic effect Effects 0.000 description 1
• 239000000185 hemagglutinin Substances 0.000 description 1
• 230000002008 hemorrhagic effect Effects 0.000 description 1
• 238000000703 high-speed centrifugation Methods 0.000 description 1
• 238000012188 high-throughput screening assay Methods 0.000 description 1
• 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
• 230000013632 homeostatic process Effects 0.000 description 1
• 238000002744 homologous recombination Methods 0.000 description 1
• 230000006801 homologous recombination Effects 0.000 description 1
• 229920001477 hydrophilic polymer Polymers 0.000 description 1
• 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
• 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
• 230000009610 hypersensitivity Effects 0.000 description 1
• 230000005965 immune activity Effects 0.000 description 1
• 230000001900 immune effect Effects 0.000 description 1
• 230000036039 immunity Effects 0.000 description 1
• 238000003018 immunoassay Methods 0.000 description 1
• 229940027941 immunoglobulin g Drugs 0.000 description 1
• 238000001114 immunoprecipitation Methods 0.000 description 1
• 230000001506 immunosuppresive effect Effects 0.000 description 1
• 239000007943 implant Substances 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 238000011503 in vivo imaging Methods 0.000 description 1
• 238000011065 in-situ storage Methods 0.000 description 1
• 230000002779 inactivation Effects 0.000 description 1
• 229910052738 indium Inorganic materials 0.000 description 1
• APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 1
• 230000006698 induction Effects 0.000 description 1
• 208000027866 inflammatory disease Diseases 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 239000003978 infusion fluid Substances 0.000 description 1
• 239000004615 ingredient Substances 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 238000002347 injection Methods 0.000 description 1
• 239000007924 injection Substances 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 229960003130 interferon gamma Drugs 0.000 description 1
• 230000000968 intestinal effect Effects 0.000 description 1
• 238000001361 intraarterial administration Methods 0.000 description 1
• 238000007918 intramuscular administration Methods 0.000 description 1
• 238000007912 intraperitoneal administration Methods 0.000 description 1
• 239000011630 iodine Substances 0.000 description 1
• 229910052740 iodine Inorganic materials 0.000 description 1
• 238000005342 ion exchange Methods 0.000 description 1
• 150000002500 ions Chemical class 0.000 description 1
• 208000037906 ischaemic injury Diseases 0.000 description 1
• 230000000302 ischemic effect Effects 0.000 description 1
• 210000004153 islets of langerhan Anatomy 0.000 description 1
• 229960000310 isoleucine Drugs 0.000 description 1
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
• 210000003734 kidney Anatomy 0.000 description 1
• 101150109249 lacI gene Proteins 0.000 description 1
• 239000002523 lectin Substances 0.000 description 1
• 229960002809 lindane Drugs 0.000 description 1
• 238000001638 lipofection Methods 0.000 description 1
• 238000009630 liquid culture Methods 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 230000004807 localization Effects 0.000 description 1
• 230000007774 longterm Effects 0.000 description 1
• 239000012931 lyophilized formulation Substances 0.000 description 1
• 210000002540 macrophage Anatomy 0.000 description 1
• 239000000594 mannitol Substances 0.000 description 1
• 235000010355 mannitol Nutrition 0.000 description 1
• HAWPXGHAZFHHAD-UHFFFAOYSA-N mechlorethamine Chemical class ClCCN(C)CCCl HAWPXGHAZFHHAD-UHFFFAOYSA-N 0.000 description 1
• 229960004961 mechlorethamine Drugs 0.000 description 1
• 201000001441 melanoma Diseases 0.000 description 1
• 238000002844 melting Methods 0.000 description 1
• 230000008018 melting Effects 0.000 description 1
• 239000003094 microcapsule Substances 0.000 description 1
• 239000002808 molecular sieve Substances 0.000 description 1
• 238000012544 monitoring process Methods 0.000 description 1
• 150000002772 monosaccharides Chemical class 0.000 description 1
• 238000002703 mutagenesis Methods 0.000 description 1
• 231100000350 mutagenesis Toxicity 0.000 description 1
• 208000010125 myocardial infarction Diseases 0.000 description 1
• 239000007922 nasal spray Substances 0.000 description 1
• 229940097496 nasal spray Drugs 0.000 description 1
• 230000017074 necrotic cell death Effects 0.000 description 1
• 239000013642 negative control Substances 0.000 description 1
• 101150091879 neo gene Proteins 0.000 description 1
• 239000002858 neurotransmitter agent Substances 0.000 description 1
• 230000003472 neutralizing effect Effects 0.000 description 1
• 239000002736 nonionic surfactant Substances 0.000 description 1
• 239000000346 nonvolatile oil Substances 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 239000002674 ointment Substances 0.000 description 1
• 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
• 150000007524 organic acids Chemical class 0.000 description 1
• 235000005985 organic acids Nutrition 0.000 description 1
• 230000002611 ovarian Effects 0.000 description 1
• 229940055729 papain Drugs 0.000 description 1
• 235000019834 papain Nutrition 0.000 description 1
• 238000007911 parenteral administration Methods 0.000 description 1
• 239000002245 particle Substances 0.000 description 1
• 229940049954 penicillin Drugs 0.000 description 1
• 229940111202 pepsin Drugs 0.000 description 1
• 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
• 238000010647 peptide synthesis reaction Methods 0.000 description 1
• 230000002093 peripheral effect Effects 0.000 description 1
• 230000002688 persistence Effects 0.000 description 1
• 238000011170 pharmaceutical development Methods 0.000 description 1
• 239000000546 pharmaceutical excipient Substances 0.000 description 1
• DDBREPKUVSBGFI-UHFFFAOYSA-N phenobarbital Chemical compound C=1C=CC=CC=1C1(CC)C(=O)NC(=O)NC1=O DDBREPKUVSBGFI-UHFFFAOYSA-N 0.000 description 1
• 229960002695 phenobarbital Drugs 0.000 description 1
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
• 101150009573 phoA gene Proteins 0.000 description 1
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
• 239000010452 phosphate Substances 0.000 description 1
• 229940080469 phosphocellulose Drugs 0.000 description 1
• 238000005222 photoaffinity labeling Methods 0.000 description 1
• 230000001766 physiological effect Effects 0.000 description 1
• 230000035790 physiological processes and functions Effects 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 229920001983 poloxamer Polymers 0.000 description 1
• 229960000502 poloxamer Drugs 0.000 description 1
• 229920000724 poly(L-arginine) polymer Polymers 0.000 description 1
• 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
• 229920000747 poly(lactic acid) Polymers 0.000 description 1
• 108010011110 polyarginine Proteins 0.000 description 1
• 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 1
• 229920000642 polymer Polymers 0.000 description 1
• 102000054765 polymorphisms of proteins Human genes 0.000 description 1
• 229920000136 polysorbate Polymers 0.000 description 1
• 229950008882 polysorbate Drugs 0.000 description 1
• 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
• 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
• 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
• 239000000843 powder Substances 0.000 description 1
• 230000002265 prevention Effects 0.000 description 1
• 230000000861 pro-apoptotic effect Effects 0.000 description 1
• 230000008569 process Effects 0.000 description 1
• 208000023958 prostate neoplasm Diseases 0.000 description 1
• 108020003519 protein disulfide isomerase Proteins 0.000 description 1
• 230000004853 protein function Effects 0.000 description 1
• 238000001742 protein purification Methods 0.000 description 1
• 239000012460 protein solution Substances 0.000 description 1
• 230000006337 proteolytic cleavage Effects 0.000 description 1
• 230000005855 radiation Effects 0.000 description 1
• 238000000163 radioactive labelling Methods 0.000 description 1
• 238000002601 radiography Methods 0.000 description 1
• 210000003370 receptor cell Anatomy 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 230000009467 reduction Effects 0.000 description 1
• 230000001105 regulatory effect Effects 0.000 description 1
• 229920005989 resin Polymers 0.000 description 1
• 239000011347 resin Substances 0.000 description 1
• 238000004007 reversed phase HPLC Methods 0.000 description 1
• 206010039073 rheumatoid arthritis Diseases 0.000 description 1
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
• 210000003705 ribosome Anatomy 0.000 description 1
• 210000003935 rough endoplasmic reticulum Anatomy 0.000 description 1
• 210000003296 saliva Anatomy 0.000 description 1
• 235000019515 salmon Nutrition 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 230000001953 sensory effect Effects 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 239000013605 shuttle vector Substances 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 238000001542 size-exclusion chromatography Methods 0.000 description 1
• 210000000813 small intestine Anatomy 0.000 description 1
• 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
• URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
• 239000001509 sodium citrate Substances 0.000 description 1
• 239000001488 sodium phosphate Substances 0.000 description 1
• 229910000162 sodium phosphate Inorganic materials 0.000 description 1
• 238000010532 solid phase synthesis reaction Methods 0.000 description 1
• 239000002904 solvent Substances 0.000 description 1
• 230000000392 somatic effect Effects 0.000 description 1
• 239000000600 sorbitol Substances 0.000 description 1
• 230000006641 stabilisation Effects 0.000 description 1
• 238000011105 stabilization Methods 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 230000000087 stabilizing effect Effects 0.000 description 1
• 239000007858 starting material Substances 0.000 description 1
• 238000011146 sterile filtration Methods 0.000 description 1
• 230000001954 sterilising effect Effects 0.000 description 1
• 238000004659 sterilization and disinfection Methods 0.000 description 1
• 238000003756 stirring Methods 0.000 description 1
• 229960005322 streptomycin Drugs 0.000 description 1
• 238000012916 structural analysis Methods 0.000 description 1
• 125000001424 substituent group Chemical group 0.000 description 1
• KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
• 150000005846 sugar alcohols Chemical class 0.000 description 1
• 229910052717 sulfur Inorganic materials 0.000 description 1
• 239000011593 sulfur Substances 0.000 description 1
• 230000001629 suppression Effects 0.000 description 1
• 239000002512 suppressor factor Substances 0.000 description 1
• 230000004083 survival effect Effects 0.000 description 1
• 239000000375 suspending agent Substances 0.000 description 1
• 238000003786 synthesis reaction Methods 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 229910052713 technetium Inorganic materials 0.000 description 1
• GKLVYJBZJHMRIY-UHFFFAOYSA-N technetium atom Chemical compound [Tc] GKLVYJBZJHMRIY-UHFFFAOYSA-N 0.000 description 1
• 238000012360 testing method Methods 0.000 description 1
• 229960002180 tetracycline Drugs 0.000 description 1
• 229930101283 tetracycline Natural products 0.000 description 1
• 235000019364 tetracycline Nutrition 0.000 description 1
• 150000003522 tetracyclines Chemical class 0.000 description 1
• 229940104230 thymidine Drugs 0.000 description 1
• 239000003104 tissue culture media Substances 0.000 description 1
• 230000002103 transcriptional effect Effects 0.000 description 1
• 238000010361 transduction Methods 0.000 description 1
• 230000026683 transduction Effects 0.000 description 1
• 238000002054 transplantation Methods 0.000 description 1
• HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
• 229940038773 trisodium citrate Drugs 0.000 description 1
• RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
• 229910052722 tritium Inorganic materials 0.000 description 1
• 239000012588 trypsin Substances 0.000 description 1
• 230000004614 tumor growth Effects 0.000 description 1
• 102000003390 tumor necrosis factor Human genes 0.000 description 1
• 239000000717 tumor promoter Substances 0.000 description 1
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
• DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
• 229940045145 uridine Drugs 0.000 description 1
• 239000004474 valine Substances 0.000 description 1
• OGWKCGZFUXNPDA-XQKSVPLYSA-N vincristine Chemical compound C([N@]1C[C@@H](C[C@]2(C(=O)OC)C=3C(=CC4=C([C@]56[C@H]([C@@]([C@H](OC(C)=O)[C@]7(CC)C=CCN([C@H]67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)C[C@@](C1)(O)CC)CC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-XQKSVPLYSA-N 0.000 description 1
• 229960004528 vincristine Drugs 0.000 description 1
• OGWKCGZFUXNPDA-UHFFFAOYSA-N vincristine Natural products C1C(CC)(O)CC(CC2(C(=O)OC)C=3C(=CC4=C(C56C(C(C(OC(C)=O)C7(CC)C=CCN(C67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)CN1CCC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-UHFFFAOYSA-N 0.000 description 1
• 230000029812 viral genome replication Effects 0.000 description 1
• 230000009385 viral infection Effects 0.000 description 1
• 239000008215 water for injection Substances 0.000 description 1
• 238000001262 western blot Methods 0.000 description 1
• 210000005253 yeast cell Anatomy 0.000 description 1
• 239000011701 zinc Substances 0.000 description 1
• 229910052725 zinc Inorganic materials 0.000 description 1