KR19990051312A - Athlete's foot eczema prevention and treatment composition containing the extract - Google Patents

Abstract

The present invention is a method for obtaining a cedar extract having a specific antimicrobial activity against Trichophyton mentagrophytes and Epidermophyton floccosum, which are the main causative agents of athlete's foot eczema, and athlete's foot eczema containing Mok's extract It relates to a composition for preventing and treating.

Description

Athlete's foot eczema prevention and treatment composition containing the extract

The present invention relates to a method for obtaining a plant extract from Mok-hyang and a composition containing the plant extract, more specifically, athlete's foot by strongly inhibiting the growth of trichophyton mentagrophyte and epidermophyton phlocosome known as the main causative agent of athlete's foot eczema. It relates to a composition for the prevention and treatment of athlete's foot eczema showing an effective effect on the prevention and treatment of eczema.

Athlete's foot eczema is one of the most common fungal infections. The causative agents of athlete's foot eczema are mainly trichophyton rubrum, trichophyton mentagrophyte and epidermophytone phlocosome among fungi (dermatophytes) that have affinity for the skin. Cause The severity of these dermatophyte infections depends on the environment of the site of infection, co-infection of other opportunistic bacteria, and personal immunity. Closure by shoes, socks, etc. increases the heat and humidity of the infected area, which promotes the growth of dermatomyte. This athlete's foot eczema is more pronounced in adults than in young children, and it is reported that 10 to 15% of the adult population in developed countries have athlete's foot or have had an infection (Clin. Exp. Dermatol. 17, 1992).

In order to treat athlete's foot eczema, a method of killing the causative agent of athlete's foot eczema or inhibiting the growth of bacteria may be considered first. It can be used in combination with steroids to eliminate dead skin cells and improve symptoms. In addition, by washing and inspecting the infected area well, lowering the heat and humidity of the infected area to create an unfavorable condition for the growth of athlete's foot fungi can also help treat athlete's foot.

As a treatment for athlete's foot eczema, many external preparations, ointments, and sprays prescribed for antifungal and salicylic acid have already been developed and sold. .

Therefore, in order to completely treat athlete's foot eczema, it is necessary to continue to apply antifungal agents and to prevent re-infection at the same time even after symptoms have improved.

In view of these problems, the present inventors have specific antibacterial effects on trichophyton mentagrophyte and epidermophytone phlocosome, known as causative agents of athlete's foot eczema, and develop various herbal medicines to develop substances having high human stability even for long-term use. And while continuing the research on plants, the extract of Mok-hyang was found to have a strong antibacterial effect at low concentrations against athlete's foot fungi and completed the present invention.

The present invention relates to an extract of Mok-hyang with excellent antimicrobial activity against causative bacteria of athlete's foot eczema and a composition for preventing and treating athlete's foot eczema containing the extract.

Mok-hyang used in the present invention is an asteraceae plant whose scientific name is Inula helenium L., in which the roots are trimmed in autumn, and the thick roots are dried. Known chemicals include allantolactone, inulin and saponin. In oriental medicine, Mokyang has been used as a placebo medicine and has been used for various respiratory diseases such as bronchitis and pulmonary tuberculosis. Mok-hyang has been used in Korean medicine for a long time. It has been confirmed to be stable to human body by ingestion. It is an extremely safe plant with no irritation to skin and mucous membranes.

The preparation method of Mokhyang extract used in the present invention is as follows.

Mokhyang medicinal herbs were ground to a size of 10 to 200 mesh, the extraction solvent was added to the pulverized Mokcho powder, cooled at room temperature for 24 hours or more, filtered, and the filtered extract solution was concentrated to obtain Mokhyang extract. The extraction solvent used here is a group consisting of purified water, methanol, ethanol, propanol, butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, ethyl acetate, benzene, hexane, diethyl ether, dichloromethane and acetone One or two or more solvents selected from were mixed and used for extraction.

The composition of the present invention contains a base as a base, and preferably contains 0.01 to 10% by weight of the extract from the neck of the tree relative to the total amount of the composition. If the extract is less than 0.01% by weight does not show a significant effect, when it exceeds 10% by weight it is not economical because the increase of the antimicrobial effect is small compared to the increase in content.

The composition of the present invention contains the extract of Mok-hyang in a common base, and in addition, skin exfoliants such as salicylic acid, alpha hydroxy acid, beta hydroxy acid, or sulfur may be added to increase the clinical effect. In addition, fragrances, pigments, fungicides, antioxidants, preservatives, moisturizers, and thickeners, inorganic salts, synthetic polymer materials and the like can be added to improve the physical properties.

Compositions such as soaps and creams prepared by including the Mok-hyang extract prepared by the above method is a composition containing the Mok-hyang extract as a result of clinical experiments to evaluate the bactericidal power and the therapeutic effect of athlete's foot eczema It was excellent in treating athlete's foot eczema without showing side effects such as skin redness.

The composition of the present invention is easy to apply to any of the different formulations, i.e. liquid, cream, paste and solid form.

Examples and experimental examples of the present invention relates to a soap and cream composition for athlete's foot eczema prevention and treatment containing Mokhyang extract obtained by the above method, these can be easily prepared by conventional manufacturing methods.

Hereinafter, the present invention will be described in detail based on Examples and Comparative Examples, but these Examples do not limit the technical scope of the present invention.

Example 1: Grinding and Extraction

500 g of dried medicinal herbs were pulverized into powder of 10 to 200 mesh size using a herbal medicine grinder, and 250 ml of 80% (v / v) ethanol aqueous solution was added thereto, followed by cooling for 24 to 48 hours at room temperature, followed by Whatman # 2 ( Whatman # 2) was filtered using filter paper and vacuum filter paper. The filtered extract was concentrated under reduced pressure at 50 캜 or lower to obtain 105 g of Mokcho extract.

Experimental Example 1: Evaluation of antimicrobial activity against causative bacteria of athlete's foot eczema

Minimum Inhibitory Concentration (MIC) Experiments on Trichophyton Mentagrophytes and Epidermophytone Flocosomes

MIC experiment was performed to test the antimicrobial activity of athlete's foot fungus of the Mokhyang extract obtained in Example 1. First, the test strain was precultured to activate. A sample solution was prepared by adding the Mokhyang extract solution to the sterilized culture at regular concentration intervals. The sample solution was inoculated with a bacterial solution of athlete's foot eczema (trichophyton mentagrophyte and epidermophytone phlocosome) previously precultured at a concentration of 1.0%. After incubating this for 3 to 4 days in a 30 ° C culture tank, the degree of growth of the bacteria was observed. As a result, MIC was about 0.01% for the causative agent of athlete's foot eczema.

Examples 2 to 6 and Comparative Example 1: Preparation of Soap Composition Containing Mokcho Extract

After mixing the neck extract and the soap base in a mixer with a composition as shown in Table 1 below, the soap composition in the form of a solid soap was prepared by extrusion, cutting and shaping in a soap manufacturing machine. The Mokmok extract can be prepared by including not only general soap, but also synthetic soap and composite soap by a conventional soap manufacturing method.

(Unit: weight%) Raw material name Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Soap base 99.995 99.99 99.90 99.00 95.00 100.00 Cedar extract 0.005 0.01 0.10 1.00 5.00 - Spices Quantity Quantity Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Quantity Quantity Antioxidant (IDT) Quantity Quantity Quantity Quantity Quantity Quantity

Experimental Example 2: Evaluation of antimicrobial activity against causative bacteria of athlete's foot eczema

In order to confirm the effect of preventing and treating athlete's foot fungus against the soap compositions prepared in Examples 2 to 6 and Comparative Example 1, the antimicrobial activity against tricophyton mentagrophyte and epidermophytone flocosum was tested as follows. .

After adding 5 wt% solution of the example composition to the mold medium, each medium was inoculated and incubated with the activated test strain. While culturing at 30 ° C. for 3 days, the growth patterns of athlete's foot fungi in the medium to which the compositions of the examples and the comparative examples were added were compared. In the Examples and Comparative Examples, five media were used to determine the average bacterial growth.

The antimicrobial activity against athlete's foot fungus was evaluated by the growth inhibition of athlete's foot fungus. If the growth of athlete's foot fungi is not inhibited, that is, if the fungus grows well because it does not contain the extract of Mok, 5, and if the fungus does not grow at all, 0, 1, 2, 3, 4, A score of 5 was given to determine the effectiveness when the average score of five media was 2 or less. The results of the experiment are shown in Table 2.

Antibacterial Activity Test Results Test composition Growth of test strain Trichophyton Mentagrophyte Epidermophyton Floco Island Example 2 4.0 4.2 Example 3 1.8 1.8 Example 4 1.0 1.2 Example 5 0.2 0.4 Example 6 0.0 0.0 Comparative Example 1 5.0 5.0

Examples 7 to 11 and Comparative Example 2: Preparation of Mokhyang Extract Containing Cream Composition

Combination of oil-based components, aqueous components, surfactants and the like and cream extract with a composition as shown in Table 3 well emulsified in a mixer, degassed, filtered, cooled to prepare a cream composition, and other additives Phosphorus preservatives, chelating agents, fragrances, and sacs were added to prepare the oil-in-water (O / W) prescription.

(Unit: weight%) Raw material name Example 7 Example 8 Example 9 Example 10 Example 11 Comparative Example 2 Stearic acid 8.00 8.00 8.00 8.00 8.00 8.00 Stearyl alcohol 4.00 4.00 4.00 4.00 4.00 4.00 Butyl stearate 6.00 6.00 6.00 6.00 6.00 6.00 Propylene glycol 5.00 5.00 5.00 5.00 5.00 5.00 Glycerin Monostearate 2.00 2.00 2.00 2.00 2.00 2.00 Potassium hydroxide 0.40 0.40 0.40 0.40 0.40 0.40 Tree extract 0.01 0.10 1.00 5.00 10.00 - Spices Quantity Quantity Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Quantity Quantity antiseptic Quantity Quantity Quantity Quantity Quantity Quantity Purified water Quantity Quantity Quantity Quantity Quantity Quantity

In addition, the cream may contain the extract of P. vulgaris in the base of the general oil-in-water (W / O), and may use fragrances, pigments, antioxidants, preservatives, etc., and synthetic or natural materials may be used to improve physical properties.

Experimental Example 3: Test of bactericidal power against causative bacteria of athlete's foot

In order to confirm the effect of preventing and treating athlete's foot eczema on the cream compositions prepared in Examples 7 to 11 and Comparative Example 2, the same experiment as in Experiment 2 was performed. The experimental results are shown in Table 4.

Test results of bactericidal power on athlete's foot fungus Test composition Growth of test strain Trichophyton Mentagrophyte Epidermophyton Floco Island Example 7 1.6 1.8 Example 8 1.2 1.4 Example 9 0.2 0.4 Example 10 0.0 0.0 Example 11 0.0 0.0 Comparative Example 2 5.0 5.0

As described above, the Mokmok extract according to the present invention exhibits excellent antimicrobial activity against the causative bacteria of athlete's foot eczema, thereby exhibiting an excellent effect in the prevention and treatment of athlete's foot eczema by using such an extract as an active ingredient of a composition such as soap and cream. I think I can.

In particular, the soap for preventing and treating athlete's foot eczema according to the present invention can be applied to the drug at the same time to wash the infection site, so that the drug is not only easy to penetrate the infection site, but also continuous drug application is the biggest problem when treating athlete's foot It has a big advantage that it can solve the hassle.

Claims (4)

Athlete's foot eczema preventing and treating composition, characterized in that it contains an extract of Moh. The method for preventing and treating athlete's foot eczema according to claim 1, which comprises 0.01 to 10% by weight of the extract of Mok-hyang. The cedar extract according to claim 1 or 2, wherein the cedar extract contains cedar as purified water, methanol, ethanol, propanol, butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, ethyl acetate, benzene, hexane, diethyl ether. , At least one mixed solvent selected from the group consisting of dichloromethane and acetone is cooled to room temperature by cooling, then filtered and concentrated to obtain a composition for preventing and treating athlete's foot eczema. The composition for preventing and treating athlete's foot eczema according to any one of claims 1 to 3, wherein the composition is liquid, cream, paste or solid.