KR102638536B1 - Skin soothing cosmetic composition containing 7 kinds of natural complexes - Google Patents

Abstract

The present invention relates to a skin soothing cosmetic composition containing complex extracts of seven natural products: Hwangbaek, Alder, Fennel, Hwangmae, Japanese herb, Schisandra chinensis, and Tangja. More specifically, it relates to a cosmetic composition containing seven types of natural complex extracts that suppress the expression of IL-1α and IL-6 mRNA, which cause inflammation by UVB exposure, and suppress the production of free radicals, thereby exhibiting an antioxidant effect.
According to the present invention, a cosmetic composition containing as an active ingredient a complex extract of seven natural products of Hwangbaek, Alder, Fennel, Hwangmae, Japanese herb, Schisandra chinensis, and Tangja, inhibits the mRNA expression of IL-1α and IL-6, which causes inflammation, and reactive oxygen species ( It is characterized by effectively suppressing the production of ROS), preventing skin inflammation, preventing aging, and having a skin soothing effect.

Description

Cosmetic composition for skin soothing containing 7 kinds of natural compounds {SKIN SOOTHING COSMETIC COMPOSITION CONTAINING 7 KINDS OF NATURAL COMPLEXES}

The present invention relates to a cosmetic composition for soothing the skin containing seven types of natural compounds, and specifically, to inhibit the expression of mRNA of inflammatory factors (IL-1α, IL-6) that cause inflammation by ultraviolet rays (UVB), thereby protecting the skin. It relates to a natural complex extract that has the effect of alleviating inflammation and a method of producing the same.

Skin is a tissue that covers the surface of the body and protects the body from physical stimuli such as shock, heat, and sunlight. It also protects the skin from chemical irritation or bacterial invasion and helps the body adapt to environmental changes by maintaining a constant body temperature. However, it is also true that as we are in close contact with the external environment, we easily become sensitive due to exposure to ultraviolet rays, environmental pollution, and chemicals.

Cytokine groups such as tumor necrosis factor (TNF-α) and interleukins (IL-1α, IL-6) are key substances in inflammatory reactions and are collectively referred to as inflammatory factors. Inflammation mediated by these inflammatory factors is one of the body's defense mechanisms that minimizes the response when cells or tissues are damaged by any cause and restores the damaged area to its original state. In addition, inflammation is the primary immune response to identify various foreign substances, such as pathogens, that have invaded the body and remove them to maintain homeostasis. However, the inflammation that occurs in this way causes damage to cells and connective tissue by proteolytic enzymes, and damage to connective tissue reduces skin elasticity, causing skin wrinkles and rapid skin aging.

Meanwhile, reactive oxygen species (ROS) refers to highly reactive radicals containing oxygen, and nitric oxide (NO) is also called reactive nitrogen species (RNS) containing nitrogen atoms (N), but is generally a subgroup of reactive oxygen species. See it as Nitric oxide or active oxygen can easily be generated due to causes such as environmental pollution, ultraviolet rays, stress, and smoking. When excessively produced, it destroys collagen and elastin, which are fibrous structures within the skin, causing it to lose elasticity, causing “wrinkles” or “sagging.” "It can cause aging symptoms such as: In addition, active oxygen acidifies the skin, causes waste products to accumulate under the skin, blocks blood circulation, and is considered a substance that promotes skin aging.

Therefore, suppressing inflammation and free radicals and soothing irritated skin are important factors in preventing skin aging. Therefore, the development of functional materials with anti-inflammatory and antioxidant effects is becoming important to prevent various skin inflammations and aging.

Korean Patent Publication No. 10-2019-0012329

The purpose of the present invention is to provide a cosmetic composition for soothing skin containing seven types of natural compounds and a method for producing the same.

Another aspect of the present invention is to provide a cosmetic composition that effectively inhibits the expression of mRNA for IL-1α and IL-6, which are inflammatory factors that cause inflammation in the skin, and the generation of free radicals directly related to skin aging.

Specifically, the cosmetic composition of the present invention confirms the excellent skin anti-inflammatory and antioxidant effects of the extract obtained by mixing 7 kinds of materials such as Hwangbaek, Alder, Fennel, Hwangmae, Japanese herb, Schisandra chinensis, and Tangja in a unique ratio, and contains these The purpose is to provide a cosmetic composition that

The technical challenges to be achieved in the embodiments are not limited to the matters mentioned above, and other technical challenges not mentioned may be considered by those skilled in the art from the various embodiments described below. You can.

To achieve this object, the present invention provides a cosmetic composition for soothing skin and preventing inflammation, comprising extracts of one or more selected from the group consisting of Hwangbaek, Alder, fennel, Hwangmae, Japanese herb, Schisandra chinensis, and Tangja.

Specifically, the cosmetic composition may be comprised of a mixed extract obtained by mixing all of the above-mentioned yellow alder, alder, fennel, yellow plum, chinensis, Schisandra chinensis, and Tangja, and more specifically, 10 to 20% by weight of yellow white, and duck. A mixed extract mixed in a ratio of 21 to 28% by weight of wood, 2 to 7% by weight of fennel, 2 to 7% by weight of yellow plum, 10 to 14% by weight of Japanese herb, 29 to 35% by weight of Schisandra chinensis, and 5 to 12% by weight of Tangja. It may have been used. In one embodiment of the present invention, it was prepared to include 15% by weight of Hwangbaek, 24% by weight of alder, 4% by weight of fennel, 4% by weight of yellow plum, 12% by weight of Japanese herb, 32% by weight of Schisandra chinensis, and 9% by weight of Tangja, It is not limited to this.

In the present invention, the hwangbaek is the dried bark of Hwangbyeok tree, a plant of the San pernia family. Hwangbyeok tree is a deciduous broad-leaved tree that grows deep in the mountains and grows to a height of about 10 m. The bark has developed cork and the inner bark is yellow. The moon-shaped, lanceolate leaves are opposite each other, and the edges of the leaves have small sawtooth and light hairs. The yellow-white berberine component has a blood pressure lowering effect, an antibacterial effect against pathogenic microorganisms, and an anti-inflammatory effect.

In the present invention, the alder tree reaches a height of 20 m, has hairs on young branches, and leaves are alternate and have a long oval shape. Alder trees live in low wetlands, are cultivated throughout Korea, and are distributed in Japan, Manchuria, and Siberia. In oriental medicine, the bark or mulberry root is used as a medicine, and is used for fever relief, hemostasis, and digestive inflammation.

In the present invention, the fennel is an annual herb of the umbel Apiaceae family and is native to the Mediterranean coast. The stem grows straight to a height of 1.5~2m and is hollow inside. The leaves are shaped like long sheaths with sharp ends and are thinly divided into 3 to 4 branches like feathers. It is used as a food or spice around the world, and has various pharmacological effects, so it is also used in oriental medicine. The phenolic compounds of fennel are known to have excellent analgesic and anti-inflammatory effects.

In the present invention, the plum is the fruit of the plum tree and is native to China. It is 5~10m tall and its leaves are alternate and egg-shaped. It has sharp sawtooth edges and hairs on both sides. It is characterized by light red flowers blooming before the leaves. Plums are rich in organic acids, which promotes sugar metabolism and relieves fatigue. It also has excellent detoxification properties and helps treat stomach upsets and food poisoning.

In the present invention, the herbaceous plant is a perennial herbaceous plant belonging to the Boraginaceae family. The stem is straight and the roots are divided into ginseng shapes. It is characterized by a strong purple color as the purple pigment of the roots is formed around the outer skin. It is characterized by the presence of purple pigment in the roots. The root has been used as a dye and medicine since ancient times, and is known to be particularly effective in treating skin inflammation such as childhood scabs, melasma, and skin rashes.

In the present invention, the name Schisandra chinensis was given because it has five tastes, and it has a harmony of sourness, sweetness, spicy, bitterness, and salty taste. It grows in Korea, Japan, China, etc. Schisandra chinensis contains ingredients such as cizandrin and gomisin, which are known to have effects such as strengthening the heart, lowering blood pressure, and increasing immunity.

In the present invention, the tangja is the fruit of the tangja tree. Tangerine is a deciduous broad-leaved shrub of the Rutaceae family. It is about 3m tall and has white flowers in May. The fruit, tangja, is round and yellow when ripe, has a good scent, but is not edible. Tangja contains essential oils such as limonene, naringin, and ponsilin. In oriental medicine, it has been used as a medicine to treat bronchial diseases.

In the present invention, the "extract" refers to a solvent into which the active ingredient contained in the extraction raw material is transferred by contacting the solvent and the extraction raw material under specific conditions. If the substance is separated from a natural product, the extraction method or ingredient All can be included regardless of the type. For example, it may include those obtained by extracting solvent-soluble components from natural products using water or an organic solvent, and those obtained by extracting only specific components such as oils from natural products, and in the present invention, essential oils in particular. However, it is not limited to this.

In addition, all formulations that can be formed using the extract itself and the extract, such as extracts derived from the natural products listed above, diluted or concentrated liquids of the extracts, dried products obtained by drying the extracts, preparations or purified products of the extracts, or mixtures thereof. It may contain extracts of Furthermore, the extract can be extracted from natural, hybrid, or mutated plants, and can also be extracted from plant tissue culture.

The extract of the present invention can be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include cold needle extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, and extrusion extraction, which may be performed alone or in combination of two or more methods.

The type of extraction solvent used in the present invention is not particularly limited, and any solvent known in the art can be used as needed. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof can be used. Specifically, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate can be used alone or in a mixture of two or more. In addition, the extract extracted as described above is filtered to remove floating solid particles, for example, by filtering out particles using nylon, etc., or by freeze filtration, and then used as is or freeze-dried, hot-air dried, or spray-dried. It can be used after drying.

In one embodiment of the present invention, 20 g of each of the seven types of natural products were separately immersed in 200 g of 70% ethanol and extracted at 60 Å for 4 hours, then filtered through filter paper (Whatman No. 2) and concentrated, and the obtained concentrate was 1.0% ( w/w) was dissolved in a solvent (30% butylene glycol, 70% purified water) and then filtered through filter paper (Durapore 0.22㎛, PVDF) to obtain natural product extracts for each of the seven natural products of the present invention. The method is not limited.

The cosmetic composition of the present invention prevents skin inflammation and aging by effectively suppressing the mRNA expression of the inflammatory factors IL-1α and IL-6 and the production of reactive oxygen species (ROS), and has a skin soothing effect. And each of these effects was confirmed through specific experimental examples (Experimental Examples 3 to 5).

In the present invention, the cosmetic composition includes shampoo, skin, toner, softening lotion, nourishing lotion, moisturizing cream, nourishing cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, mist, It may be formulated with one or more selected from the group consisting of spray and powder. Specifically, the cosmetic composition may be formulated in a shampoo form, but is not limited thereto.

The cosmetic composition can be formulated by conventional methods. In formulating external skin preparations, reference may be made to information disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, and in formulating cosmetic compositions, reference may be made to International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition can be prepared in a general emulsified formulation and solubilized formulation. For example, lotion such as softening lotion or nourishing lotion; Emulsions such as facial lotion and body lotion; Creams such as nutritional cream, moisture cream, eye cream, etc.; essence; cosmetic ointment; spray; gel; pack; sunscreen; makeup base; Foundation, such as liquid type, solid type, or spray type; powder; Make-up removers such as cleansing creams, cleansing lotions, and cleansing oils; Alternatively, it may be formulated as a cleansing agent such as cleansing foam, soap, or body wash, but is not limited to this. Additionally, the cosmetic composition may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.

In addition to the essential ingredients, in each formulation, other ingredients may be appropriately mixed in the cosmetic composition, depending on the type of formulation or purpose of use, within the range that does not impair the purpose of the present invention.

The cosmetic composition may contain a generally acceptable carrier and may be appropriately mixed with, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc., but is not limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated into ointments, pastes, creams or gels, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or these are used as carrier ingredients. mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as carrier ingredients, and in the case of a spray, chlorofluorohydride may be used. It may further include propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the cosmetic composition is formulated as a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the cosmetic composition is formulated into soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. are used as carrier ingredients. can be used

The cosmetic composition may contain fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, and fragrances commonly used in the industry depending on the quality or function of the final product. , surfactants, water, ionic or non-ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics. It may additionally contain auxiliaries commonly used in the fields of cosmetology or dermatology, such as any other ingredients.

However, the auxiliaries and their mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

According to the present invention, a cosmetic composition containing as an active ingredient a complex extract of seven natural products of Hwangbaek, Alder, Fennel, Hwangmae, Japanese herb, Schisandra chinensis, and Tangja, inhibits the mRNA expression of IL-1α and IL-6, which causes inflammation, and active oxygen radicals. It is characterized by effectively suppressing the production of (ROS), preventing skin inflammation, preventing aging, and having a skin soothing effect.

In addition, the cosmetic composition of the present invention can be used for shampoo, skin, toner suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, and It is useful for use in various formulations such as sprays.

In addition, the present invention is characterized by containing extracts derived from natural products, so that it is harmless to the human body and can be easily applied to products.

The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

The accompanying drawings, which are included as part of the detailed description to aid understanding of the embodiments, provide various embodiments and together with the detailed description describe technical features of the various embodiments.
Figure 1 shows the inhibitory effect on IL-1α expression of the complex extract according to Examples 2 to 5 of the present invention.
Figure 2 shows the inhibitory effect of IL-6 expression of the complex extract according to Examples 2 to 5 of the present invention.
Figure 3 shows the ROS production inhibition effect of the complex extract according to Examples 2 to 5 of the present invention.

The following embodiments combine elements and features of the embodiments in a predetermined form. Each component or feature may be considered optional unless explicitly stated otherwise. Each component or feature may be implemented in a form that is not combined with other components or features. Additionally, various embodiments may be configured by combining some components and/or features. The order of operations described in various embodiments may change. Some features or features of one embodiment may be included in other embodiments or may be replaced with corresponding features or features of other embodiments.

Example 1. Preparation of natural product extract

The cosmetic composition of the present invention was prepared using seven types of natural products, and extracts of these were prepared respectively.

Specifically, 7 types of natural products, Schisandra chinensis, Alder tree, Hwangbaek, Japanese herb, Tangja, Fennel, and Yellow Plum, were each prepared in a dried state, and 20g of each of these natural products were separately immersed in 200g of 70% ethanol and incubated at 60Å for 4 hours. After extraction, it was filtered through filter paper (Whatman No.2) and concentrated. The obtained concentrate was dissolved in a solvent (30% butylene glycol, 70% purified water) to make it 1.0% (w/w), and then filtered through filter paper (Durapore 0.22㎛, PVDF) to obtain the natural products for each of the seven natural products of the present invention. An extract was obtained.

Experimental Example 1. Confirmation of skin soothing effect through inhibition of IL-1α activity of individual materials

It was evaluated whether each of the natural product extracts showed an effect of suppressing skin inflammation by inhibiting the expression of interleukin-1α (IL-1α).

Specifically, an IL-1α gene expression experiment was performed to confirm the sedative efficacy. HaCaT (immortalized keratinocyte) cells were dispensed into 60 mm dishes and cultured for 24 hours. Replace with serum-free medium, irradiate HaCaT with Ultraviolet B (UVB), which causes inflammatory reactions such as sunburn, erythema, and edema, at 50 mJ/㎠, and then treat the samples by concentration for 4 hours at 37°C, CO ₂ 5 % Cultured in incubator. After culturing, cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the extracted total RNA, cDNA was synthesized from 1 μg of RNA using a cDNA synthesis kit using a ProFlex PCR system (Applied Biosystems). To confirm gene expression, the 7500 fast real-time PCR system (Applied Biosystems) was used and the results are shown in Table 1 below.

As a result of the cytotoxicity evaluation, it was confirmed that Schisandra chinensis, alder, Hwangbaek, and Japanese herb were not toxic when treated at a concentration of 1%, and in the case of Tangja, fennel, and Hwangmae at a concentration of 0.5%, and experiments were conducted accordingly.

Sample UVB Concentration (%) IL-1α mRNA expression inhibition rate (%) Control - - 93.6 UVB 50mJ - 0.0 Quercetin (μM) 50mJ 25 34.2 Schisandra 50mJ 1.0 82.8 alder tree 50mJ 1.0 62.6 yellow-white 50mJ 1.0 43.4 self-inflicted 50mJ 1.0 43.8 Tangja 50mJ 0.5 37.8 fennel 50mJ 0.5 24.6 yellow plum 50mJ 0.5 28.2

As a result of the evaluation of inhibition of IL-1α expression, as shown in Table 1, Schisandra chinensis showed the best inhibition activity of IL-1α expression at 82.8%. It was confirmed that all seven types of materials were effective in inhibiting IL-1α expression, and in particular, Schisandra chinensis, Alder tree, Hwangbaek, Japanese herb, and Tangja showed a superior inhibition rate than quercetin, a positive control.

Experimental Example 2. Confirmation of skin soothing effect through inhibition of IL-6 activity of individual materials]

It was evaluated whether each of the natural product extracts showed an effect of suppressing skin inflammation by inhibiting the expression of interleukin-6 (IL-6).

Specifically, an IL-6 gene expression experiment was performed to confirm the sedative efficacy. HaCaT (immortalized keratinocyte) cells were distributed on 60mm dishes and cultured for 24 hours, then replaced with serum-free medium and 50 mJ/㎠ of Ultraviolet B (UVB), which causes inflammatory reactions such as sunburn, erythema, and edema, in HaCaT. After irradiation, the samples were treated according to concentration and incubated in an incubator at 37°C and CO ₂ 5% for 4 hours. After culturing, cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the extracted total RNA, cDNA was synthesized from 1 μg of RNA using a cDNA synthesis kit using a ProFlex PCR system (Applied Biosystems). Gene expression was confirmed using the 7500 fast real-time PCR system (Applied Biosystems), and the results are shown in Table 2 below.

As a result of the cytotoxicity evaluation, it was confirmed that Schisandra chinensis, alder, Hwangbaek, and Japanese apricots were not toxic when treated at a concentration of 1%, and in the case of Tangja, fennel, and Hwangmaesil, when treated at a concentration of 0.5%, the experiment was conducted accordingly.

Sample UVB Concentration (%) IL-6 mRNA expression inhibition rate Control - - 3.2 UVB 50mJ - 100.0 Quercetin (μM) 50mJ 25 44.4 Schisandra 50mJ 1.0 82.0 alder tree 50mJ 1.0 77.4 yellow-white 50mJ 1.0 46.5 self-inflicted 50mJ 1.0 42.1 Tangja 50mJ 0.5 31.6 fennel 50mJ 0.5 39.0 yellow plum 50mJ 0.5 40.1

As a result of the evaluation of inhibition of IL-6 expression, as shown in Table 2, Schisandra chinensis showed the best activity at 82.0%. It was confirmed that all seven types of materials were effective in suppressing IL-6 expression, and in particular, Schisandra chinensis, Alder tree, and Hwangbaek showed a higher inhibition rate of interleukin-6 expression than the positive control quercetin.

Examples 2 to 5. Preparation of 7 types of complex extracts

By mixing according to the weight ratio in Table 3 below, the complex extracts of Examples 2 to 5 of the present invention were prepared.

The complex extract of Example 2 was prepared by mixing 7 types of materials in equal proportions, and in the case of Examples 3 to 5, considering the results of Experimental Examples 1 and 2 to show the best anti-inflammatory effect, the extracts in Table 3 below were prepared. It was extracted by mixing in the same weight ratio.

50 g of 7 materials mixed according to the ratio of Examples 2 to 5 were immersed in 500 g of 70% ethanol and extracted at 60 Å for 4 hours, then filtered through filter paper (Whatman No. 2) and concentrated. The obtained concentrate was dissolved in a solvent (30% butylene glycol, 70% purified water) to make it 1.0% (w/w), then filtered through filter paper (Durapore 0.22㎛, PVDF) before use.

Raw materials Example 2 Example 3 Example 4 Example 5 Schisandra 14.3% 32.0% 28.5% 40.0% alder tree 14.3% 24.0% 28.5% 20.0% yellow-white 14.3% 15.0% 14.3% 10.0% self-inflicted 14.3% 12.0% 14.3% 9.0% Tangja 14.3% 9.0% 4.8% 7.0% fennel 14.3% 4.0% 4.8% 7.0% yellow plum 14.3% 4.0% 4.8% 7.0%

Experimental Example 3. Confirmation of skin soothing effect through inhibition of IL-1α activity of complex extract

In order to evaluate whether the complex extracts of Examples 2 to 5 are effective in suppressing skin inflammation by inhibiting the expression of IL-1α, an experiment was performed in the same manner as Experiment 1, and the results are shown in Table 4 below.

Sample UVB Concentration (%) IL-1α inhibition rate (%) Control - - 93.6 UVB 50mJ - 0.0 Quercetin (μM) 50mJ 25 34.2 Example 2 50mJ 3.0 11.6 Example 3 50mJ 3.0 38.2 Example 4 50mJ 3.0 24.7 Example 5 50mJ 3.0 27.3

As shown in Table 4, in the case of Example 3, an inhibition rate of 38.2% was confirmed, showing an inhibition rate close to that of the positive control group. On the other hand, Example 2 showed a low inhibition rate of 11.6%. In addition, it was confirmed that the ratio of Example 3 showed a significantly better effect than the results of Example 5, which contained the most Schisandra chinensis, which was the most effective on its own, or Example 4, which contained more alder.

Experimental Example 4. Confirmation of skin soothing effect through inhibition of IL-6 activity of complex extract

In order to evaluate whether the complex extracts of Examples 2 to 5 are effective in suppressing skin inflammation by inhibiting the expression of IL-6, an experiment was performed in the same manner as Experiment 2, and the results are shown in Table 5 below.

Sample UVB Concentration (%) IL-6 inhibition rate Control - - 95.8 UVB 50mJ - 0.0 Quercetin (μM) 50mJ 25 46.5 Example 2 50mJ 3.0 - Example 3 50mJ 3.0 22.9 Example 4 50mJ 3.0 10.8 Example 5 50mJ 3.0 12.1

As shown in Table 5, in the case of Example 3, an inhibition rate of 22.9% was confirmed, whereas in the case of Example 2, the expression rate was 113.2%, which showed that the expression of IL-6 was promoted. In addition, the results of Examples 4 and 5 showed lower effectiveness than Example 3, which is similar to Experimental Example 3, considering that the content of Schisandra chinensis and Alder tree, which showed the best effect as a single raw material, increased. It can be seen that this is the result of a synergy effect according to the mixing ratio in Example 3.

Experimental Example 5. Confirmation of antioxidant effect through inhibition of ROS production

In order to evaluate whether the complex extracts of Examples 2 to 5 exhibit an antioxidant effect by inhibiting the production of reactive oxygen species (ROS), an experiment was conducted in the following manner.

Reactive Oxygen Species (ROS) Assay was performed to confirm antioxidant efficacy. HaCaT (immortalized keratinocyte) cells were dispensed into a 96 well plate and cultured for 24 hours, then replaced with serum-free medium, samples were treated according to concentration, and cultured in a 5% CO ₂ incubator at 37°C for 24 hours. After culturing, the cells were washed with HBSS, treated with H ₂ DcFDA solution, blocked from light, and incubated in an incubator for 30 minutes. After washing with HBSS, it was treated with 100 mM H ₂ O ₂ solution and reacted in an incubator for 10 minutes. Then, the fluorescence value was measured to determine the amount of ROS produced. The results are shown in Table 6 below.

Sample Concentration (%) ROS generation inhibition rate (%) Control - 0.0 EGCG (ppm) 1.0 26.48 Example 2 0.75 4.56 Example 3 0.75 19.23 Example 4 0.75 7.50 Example 5 0.75 9.87

As shown in Table 6, Example 3 showed an inhibition rate of 19.23%, while Example 2 showed a low inhibition rate of 4.56%. In addition, Example 4 was 7.50% and Example 5 was 9.87%, which was higher than Example 2, but it was confirmed that the effect was only about half that of Example 3. Considering the results of each experiment, the present invention It can be confirmed that the seven types of complex extracts and the cosmetic composition containing them can effectively suppress the expression of mRNA of the inflammatory factors IL-1α and IL-6 and the generation of reactive oxygen species directly related to skin aging.

The various embodiments described above may be embodied in other specific forms without departing from the technical idea and essential features. Accordingly, the above detailed description should not be construed as restrictive in all respects and should be considered illustrative. The scope of the various embodiments should be determined by a reasonable interpretation of the appended claims, and all changes within the equivalent scope of the various embodiments are included in the scope of the various embodiments. In addition, claims that do not have an explicit reference relationship in the patent claims can be combined to form an embodiment or included as a new claim through amendment after filing.

Claims (5)

10 to 20% by weight of Hwangbaek, 21 to 28% by weight of Alder, 2 to 7% by weight of Fennel, 2 to 7% by weight of Yellow Plum, 10 to 14% by weight of Japanese herb, 29 to 35% by weight of Schisandra chinensis and 5 to 12% by weight of Tangja. Contains extracts extracted by mixing in a ratio of
Characterized by suppressing skin troubles and inflammation caused by the expression of IL-1α and IL-6,
A cosmetic composition for soothing skin and preventing inflammation.
delete delete delete The method of claim 1, wherein the cosmetic composition further has an antioxidant effect by suppressing the production of reactive oxygen species (ROS).
A cosmetic composition for soothing skin and preventing inflammation.