KR102843262B1 - 자가-발광을 기초로 하는 단일-채널 시퀀싱 방법 - Google Patents

자가-발광을 기초로 하는 단일-채널 시퀀싱 방법

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Publication number
KR102843262B1
KR102843262B1 KR1020217040845A KR20217040845A KR102843262B1 KR 102843262 B1 KR102843262 B1 KR 102843262B1 KR 1020217040845 A KR1020217040845 A KR 1020217040845A KR 20217040845 A KR20217040845 A KR 20217040845A KR 102843262 B1 KR102843262 B1 KR 102843262B1
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South Korea
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nucleic acid
compound
molecular
luciferase
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Korean (ko)
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KR20220009992A (ko
Inventor
샤 리아오
시 첸
아오 첸
웬웨이 장
총준 수
홍민 첸
지에 자오
데펑 푸
Original Assignee
칭다오 엠쥐아이 테크 컴퍼니 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020217040845A 2019-05-15 2019-05-15 자가-발광을 기초로 하는 단일-채널 시퀀싱 방법 Active KR102843262B1 (ko)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/086974 WO2020227953A1 (zh) 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法

Publications (2)

Publication Number Publication Date
KR20220009992A KR20220009992A (ko) 2022-01-25
KR102843262B1 true KR102843262B1 (ko) 2025-08-06

Family

ID=73289989

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020217040845A Active KR102843262B1 (ko) 2019-05-15 2019-05-15 자가-발광을 기초로 하는 단일-채널 시퀀싱 방법

Country Status (12)

Country Link
US (1) US12565677B2 (https=)
EP (1) EP3988670B1 (https=)
JP (2) JP7485483B2 (https=)
KR (1) KR102843262B1 (https=)
CN (2) CN118931711A (https=)
AU (1) AU2019445584B2 (https=)
BR (1) BR112021022809A2 (https=)
CA (1) CA3139169A1 (https=)
MX (1) MX2021013983A (https=)
SG (1) SG11202111778YA (https=)
WO (1) WO2020227953A1 (https=)
ZA (1) ZA202110159B (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021031109A1 (zh) * 2019-08-20 2021-02-25 深圳华大智造极创科技有限公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
TW202342494A (zh) 2022-03-30 2023-11-01 日商肽夢想股份有限公司 具有TrkB結合活性之肽複合物
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
WO2024050815A1 (zh) 2022-09-09 2024-03-14 深圳华大生命科学研究院 杂芳环化合物在核酸检测中的用途
US20250297306A1 (en) 2022-09-28 2025-09-25 Mgi Tech Co., Ltd. Sequencing method
WO2024098185A1 (en) 2022-11-07 2024-05-16 GeneSense Technology Inc., Shanghai (CN) Nucleic acid sequencing using self-luminescence
WO2024108390A1 (zh) 2022-11-22 2024-05-30 深圳华大智造科技股份有限公司 基因测序芯片、封装结构、系统及清洗、制作、测序方法

Citations (2)

* Cited by examiner, † Cited by third party
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WO2016040607A1 (en) * 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法

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CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
JP2004510433A (ja) 2000-10-06 2004-04-08 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク Ｄｎａおよびｒｎａを解読するための大量並行方法
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
CN1854308A (zh) 2005-04-21 2006-11-01 株式会社日立制作所 碱基序列检测方法
CA2859660C (en) * 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
WO2017087823A1 (en) * 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
EP3376997B1 (en) 2015-11-20 2024-11-13 Pacific Biosciences of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
US10851410B2 (en) 2017-01-04 2020-12-01 Mgi Tech Co., Ltd. Nucleic acid sequencing using affinity reagents
BR112019014683A2 (pt) 2017-03-07 2020-02-18 Illumina, Inc. Sequenciamento com dois canais óticos, com fonte de luz única
CN110650968B (zh) * 2017-10-11 2022-07-05 深圳华大智造科技股份有限公司 修饰的核苷或核苷酸

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016040607A1 (en) * 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法

Also Published As

Publication number Publication date
CN113748216B (zh) 2024-04-23
ZA202110159B (en) 2022-08-31
CN113748216A (zh) 2021-12-03
KR20220009992A (ko) 2022-01-25
EP3988670A1 (en) 2022-04-27
AU2019445584B2 (en) 2024-09-05
EP3988670B1 (en) 2026-04-22
MX2021013983A (es) 2022-02-10
WO2020227953A1 (zh) 2020-11-19
EP3988670A4 (en) 2023-01-25
CN118931711A (zh) 2024-11-12
CA3139169A1 (en) 2020-11-19
BR112021022809A2 (pt) 2022-01-25
SG11202111778YA (en) 2021-12-30
JP7485483B2 (ja) 2024-05-16
AU2019445584A1 (en) 2022-01-20
JP7825660B2 (ja) 2026-03-06
US12565677B2 (en) 2026-03-03
US20220205036A1 (en) 2022-06-30
JP2022533916A (ja) 2022-07-27
JP2024099789A (ja) 2024-07-25

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