ZA202110159B - Single-channel sequencing method based on self-luminescence - Google Patents

Single-channel sequencing method based on self-luminescence

Info

Publication number
ZA202110159B
ZA202110159B ZA2021/10159A ZA202110159A ZA202110159B ZA 202110159 B ZA202110159 B ZA 202110159B ZA 2021/10159 A ZA2021/10159 A ZA 2021/10159A ZA 202110159 A ZA202110159 A ZA 202110159A ZA 202110159 B ZA202110159 B ZA 202110159B
Authority
ZA
South Africa
Prior art keywords
luminescence
self
method based
sequencing method
channel sequencing
Prior art date
Application number
ZA2021/10159A
Other languages
English (en)
Inventor
Sha Liao
Xi Chen
Ao Chen
Wenwei Zhang
Chongjun Xu
Hongmin Chen
Jie Zhao
Defeng Fu
Original Assignee
Egi Tech Shen Zhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Egi Tech Shen Zhen Co Ltd filed Critical Egi Tech Shen Zhen Co Ltd
Publication of ZA202110159B publication Critical patent/ZA202110159B/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA2021/10159A 2019-05-15 2021-12-08 Single-channel sequencing method based on self-luminescence ZA202110159B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/086974 WO2020227953A1 (zh) 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法

Publications (1)

Publication Number Publication Date
ZA202110159B true ZA202110159B (en) 2022-08-31

Family

ID=73289989

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2021/10159A ZA202110159B (en) 2019-05-15 2021-12-08 Single-channel sequencing method based on self-luminescence

Country Status (12)

Country Link
US (1) US12565677B2 (https=)
EP (1) EP3988670B1 (https=)
JP (2) JP7485483B2 (https=)
KR (1) KR102843262B1 (https=)
CN (2) CN118931711A (https=)
AU (1) AU2019445584B2 (https=)
BR (1) BR112021022809A2 (https=)
CA (1) CA3139169A1 (https=)
MX (1) MX2021013983A (https=)
SG (1) SG11202111778YA (https=)
WO (1) WO2020227953A1 (https=)
ZA (1) ZA202110159B (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021031109A1 (zh) * 2019-08-20 2021-02-25 深圳华大智造极创科技有限公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
TW202342494A (zh) 2022-03-30 2023-11-01 日商肽夢想股份有限公司 具有TrkB結合活性之肽複合物
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
WO2024050815A1 (zh) 2022-09-09 2024-03-14 深圳华大生命科学研究院 杂芳环化合物在核酸检测中的用途
US20250297306A1 (en) 2022-09-28 2025-09-25 Mgi Tech Co., Ltd. Sequencing method
WO2024098185A1 (en) 2022-11-07 2024-05-16 GeneSense Technology Inc., Shanghai (CN) Nucleic acid sequencing using self-luminescence
WO2024108390A1 (zh) 2022-11-22 2024-05-30 深圳华大智造科技股份有限公司 基因测序芯片、封装结构、系统及清洗、制作、测序方法

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CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
JP2004510433A (ja) 2000-10-06 2004-04-08 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク Dnaおよびrnaを解読するための大量並行方法
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
CN1854308A (zh) 2005-04-21 2006-11-01 株式会社日立制作所 碱基序列检测方法
CA2859660C (en) * 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
EP3191606B1 (en) * 2014-09-12 2020-05-27 Illumina, Inc. Methods for detecting the presence of polymer subunits using chemiluminescence
WO2017087823A1 (en) * 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
EP3376997B1 (en) 2015-11-20 2024-11-13 Pacific Biosciences of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
EP3564387A4 (en) * 2016-12-27 2020-10-07 EGI Tech (Shen Zhen) Co., Limited UNIQUE FLUORESCENT DYE SEQUENCING PROCESS
US10851410B2 (en) 2017-01-04 2020-12-01 Mgi Tech Co., Ltd. Nucleic acid sequencing using affinity reagents
BR112019014683A2 (pt) 2017-03-07 2020-02-18 Illumina, Inc. Sequenciamento com dois canais óticos, com fonte de luz única
CN110650968B (zh) * 2017-10-11 2022-07-05 深圳华大智造科技股份有限公司 修饰的核苷或核苷酸

Also Published As

Publication number Publication date
CN113748216B (zh) 2024-04-23
CN113748216A (zh) 2021-12-03
KR102843262B1 (ko) 2025-08-06
KR20220009992A (ko) 2022-01-25
EP3988670A1 (en) 2022-04-27
AU2019445584B2 (en) 2024-09-05
EP3988670B1 (en) 2026-04-22
MX2021013983A (es) 2022-02-10
WO2020227953A1 (zh) 2020-11-19
EP3988670A4 (en) 2023-01-25
CN118931711A (zh) 2024-11-12
CA3139169A1 (en) 2020-11-19
BR112021022809A2 (pt) 2022-01-25
SG11202111778YA (en) 2021-12-30
JP7485483B2 (ja) 2024-05-16
AU2019445584A1 (en) 2022-01-20
JP7825660B2 (ja) 2026-03-06
US12565677B2 (en) 2026-03-03
US20220205036A1 (en) 2022-06-30
JP2022533916A (ja) 2022-07-27
JP2024099789A (ja) 2024-07-25

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