CN113748216A - 一种基于自发光的单通道测序方法 - Google Patents

一种基于自发光的单通道测序方法 Download PDF

Info

Publication number
CN113748216A
CN113748216A CN201980095781.8A CN201980095781A CN113748216A CN 113748216 A CN113748216 A CN 113748216A CN 201980095781 A CN201980095781 A CN 201980095781A CN 113748216 A CN113748216 A CN 113748216A
Authority
CN
China
Prior art keywords
nucleic acid
attached
compound
luciferase
molecular tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201980095781.8A
Other languages
English (en)
Other versions
CN113748216B (zh
Inventor
廖莎
陈曦
陈奥
章文蔚
徐崇钧
陈宏敏
赵杰
傅德丰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Huada Zhizao Technology Co ltd
Original Assignee
Shenzhen Huada Zhizaojichuang Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Huada Zhizaojichuang Technology Co ltd filed Critical Shenzhen Huada Zhizaojichuang Technology Co ltd
Publication of CN113748216A publication Critical patent/CN113748216A/zh
Application granted granted Critical
Publication of CN113748216B publication Critical patent/CN113748216B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明提供了一种基于单荧光染料的测序方法，其中利用自发光的信号来分辨不同核苷酸的依次掺入，从而实现多核苷酸序列的测定。

Description

PCT国内申请，说明书已公开。

Claims (12)

  1. PCT国内申请，权利要求书已公开。
CN201980095781.8A 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法 Active CN113748216B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/086974 WO2020227953A1 (zh) 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法

Publications (2)

Publication Number Publication Date
CN113748216A true CN113748216A (zh) 2021-12-03
CN113748216B CN113748216B (zh) 2024-04-23

Family

ID=73289989

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980095781.8A Active CN113748216B (zh) 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法

Country Status (12)

Country Link
US (1) US20220205036A1 (zh)
EP (1) EP3988670A4 (zh)
JP (1) JP7485483B2 (zh)
KR (1) KR20220009992A (zh)
CN (1) CN113748216B (zh)
AU (1) AU2019445584A1 (zh)
BR (1) BR112021022809A2 (zh)
CA (1) CA3139169A1 (zh)
MX (1) MX2021013983A (zh)
SG (1) SG11202111778YA (zh)
WO (1) WO2020227953A1 (zh)
ZA (1) ZA202110159B (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024067674A1 (zh) * 2022-09-28 2024-04-04 深圳华大智造科技股份有限公司 测序方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019462785A1 (en) * 2019-08-20 2022-04-07 Qingdao MGI Tech Co. Ltd Method for sequencing polynucleotides on basis of optical signal dynamics of luminescent label and secondary luminescent signal
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
WO2024098185A1 (en) 2022-11-07 2024-05-16 GeneSense Technology Inc., Shanghai (CN) Nucleic acid sequencing using self-luminescence

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130079232A1 (en) * 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2016040607A1 (en) * 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法
US20180223358A1 (en) * 2017-01-04 2018-08-09 Complete Genomics, Inc. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
CN108603227A (zh) * 2015-11-18 2018-09-28 卡利姆·U·米尔 超分辨率测序
WO2019071474A1 (zh) * 2017-10-11 2019-04-18 深圳华大智造科技有限公司 修饰的核苷或核苷酸

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
EP1105529B2 (en) 1998-07-30 2013-05-29 Illumina Cambridge Limited Arrayed biomolecules and their use in sequencing
CA2425112C (en) 2000-10-06 2011-09-27 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
EP3673986A1 (en) 2004-01-07 2020-07-01 Illumina Cambridge Limited Improvements in or relating to molecular arrays
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
CN1854308A (zh) * 2005-04-21 2006-11-01 株式会社日立制作所 碱基序列检测方法
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
CN113321943A (zh) * 2015-11-20 2021-08-31 加利福尼亚太平洋生物科学股份有限公司 标记的核苷酸类似物、反应混合物以及测序方法和系统

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130079232A1 (en) * 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2016040607A1 (en) * 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
CN108603227A (zh) * 2015-11-18 2018-09-28 卡利姆·U·米尔 超分辨率测序
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法
US20180223358A1 (en) * 2017-01-04 2018-08-09 Complete Genomics, Inc. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
WO2019071474A1 (zh) * 2017-10-11 2019-04-18 深圳华大智造科技有限公司 修饰的核苷或核苷酸

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SARA GOODWIN: "Coming of age: ten years of next-generation sequencing technologies", NAT REV GENET, vol. 17, no. 6, pages 333 - 351, XP055544186, DOI: 10.1038/nrg.2016.49 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024067674A1 (zh) * 2022-09-28 2024-04-04 深圳华大智造科技股份有限公司 测序方法

Also Published As

Publication number Publication date
US20220205036A1 (en) 2022-06-30
JP7485483B2 (ja) 2024-05-16
SG11202111778YA (en) 2021-12-30
AU2019445584A1 (en) 2022-01-20
MX2021013983A (es) 2022-02-10
BR112021022809A2 (pt) 2022-01-25
KR20220009992A (ko) 2022-01-25
CA3139169A1 (en) 2020-11-19
WO2020227953A1 (zh) 2020-11-19
ZA202110159B (en) 2022-08-31
EP3988670A4 (en) 2023-01-25
CN113748216B (zh) 2024-04-23
JP2022533916A (ja) 2022-07-27
EP3988670A1 (en) 2022-04-27

Similar Documents

Publication Publication Date Title
US10808244B2 (en) Method of normalizing biological samples
CN113748216B (zh) 一种基于自发光的单通道测序方法
US20060166203A1 (en) New sequencing method for sequencing rna molecules
CN114286867B (zh) 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
CN112840035B (zh) 对多核苷酸进行测序的方法
WO2020073274A1 (zh) 对多核苷酸进行测序的方法
RU2794177C1 (ru) Способ одноканального секвенирования на основе самолюминесценции
JP2005512544A (ja) 核酸の合成後ラベリングとその使用
JP2005512544A6 (ja) 核酸の合成後ラベリングとその使用
US11952624B1 (en) Nucleic acid sequencing using self-luminescence
JP5218944B2 (ja) 核酸を配列特異的に標識する方法、およびそれを利用した新規核酸検出法
EP4363613A1 (en) Detecting methylcytosine using a modified base opposite to the methylcytosine
WO2004029294A1 (en) New sequencing method for sequencing rna molecules

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40055932

Country of ref document: HK

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20231016

Address after: Building 4, No. 2 Hengyunshan Road, Qingdao Area, China (Shandong) Pilot Free Trade Zone, Qingdao City, Shandong Province, 266599

Applicant after: Qingdao Huada Zhizao Technology Co.,Ltd.

Address before: 518083 7th floor, building 11, Beishan Industrial Zone, 146 Beishan Road, Yangang community, Yantian street, Yantian District, Shenzhen City, Guangdong Province

Applicant before: Shenzhen Huada zhizaojichuang Technology Co.,Ltd.

GR01 Patent grant
GR01 Patent grant