MX2021013983A - Metodo de secuenciacion monocanal basado en autoluminiscencia. - Google Patents

Metodo de secuenciacion monocanal basado en autoluminiscencia.

Info

Publication number
MX2021013983A
MX2021013983A MX2021013983A MX2021013983A MX2021013983A MX 2021013983 A MX2021013983 A MX 2021013983A MX 2021013983 A MX2021013983 A MX 2021013983A MX 2021013983 A MX2021013983 A MX 2021013983A MX 2021013983 A MX2021013983 A MX 2021013983A
Authority
MX
Mexico
Prior art keywords
self
method based
sequencing method
luminescence
channel sequencing
Prior art date
Application number
MX2021013983A
Other languages
English (en)
Inventor
Xi Chen
Jie Zhao
Ao Chen
Sha Liao
Chongjun Xu
Wenwei Zhang
Hongmin Chen
Defeng Fu
Original Assignee
Egi Tech Shen Zhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Egi Tech Shen Zhen Co Ltd filed Critical Egi Tech Shen Zhen Co Ltd
Publication of MX2021013983A publication Critical patent/MX2021013983A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Abstract

La presente invención proporciona un método de secuenciación basado en un tinte fluorescente único, en donde una señal de autoluminiscencia se utiliza para distinguir la incorporación secuencial de diferentes nucleótidos, realizando así la determinación de la secuencia polinucleotídica.
MX2021013983A 2019-05-15 2019-05-15 Metodo de secuenciacion monocanal basado en autoluminiscencia. MX2021013983A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/086974 WO2020227953A1 (zh) 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法

Publications (1)

Publication Number Publication Date
MX2021013983A true MX2021013983A (es) 2022-02-10

Family

ID=73289989

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021013983A MX2021013983A (es) 2019-05-15 2019-05-15 Metodo de secuenciacion monocanal basado en autoluminiscencia.

Country Status (11)

Country Link
US (1) US20220205036A1 (es)
EP (1) EP3988670A4 (es)
JP (1) JP2022533916A (es)
KR (1) KR20220009992A (es)
AU (1) AU2019445584A1 (es)
BR (1) BR112021022809A2 (es)
CA (1) CA3139169A1 (es)
MX (1) MX2021013983A (es)
SG (1) SG11202111778YA (es)
WO (1) WO2020227953A1 (es)
ZA (1) ZA202110159B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114286867B (zh) * 2019-08-20 2024-04-16 青岛华大智造科技有限责任公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
US11952624B1 (en) 2022-11-07 2024-04-09 GeneSense Technology Inc., Shanghai (CN) Nucleic acid sequencing using self-luminescence

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
CA2339121A1 (en) 1998-07-30 2000-02-10 Shankar Balasubramanian Arrayed biomolecules and their use in sequencing
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
SI3587433T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
CN1854308A (zh) * 2005-04-21 2006-11-01 株式会社日立制作所 碱基序列检测方法
US9593373B2 (en) 2013-03-15 2017-03-14 Illumina Cambridge Limited Modified nucleosides or nucleotides
EP3191606B1 (en) * 2014-09-12 2020-05-27 Illumina, Inc. Methods for detecting the presence of polymer subunits using chemiluminescence
CN108697499B (zh) * 2015-11-20 2021-06-22 加利福尼亚太平洋生物科学股份有限公司 标记的核苷酸类似物、反应混合物以及测序方法和系统
RU2760737C2 (ru) * 2016-12-27 2021-11-30 Еги Тек (Шэнь Чжэнь) Ко., Лимитед Способ секвенирования на основе одного флуоресцентного красителя

Also Published As

Publication number Publication date
KR20220009992A (ko) 2022-01-25
ZA202110159B (en) 2022-08-31
EP3988670A4 (en) 2023-01-25
US20220205036A1 (en) 2022-06-30
WO2020227953A1 (zh) 2020-11-19
AU2019445584A1 (en) 2022-01-20
BR112021022809A2 (pt) 2022-01-25
EP3988670A1 (en) 2022-04-27
CA3139169A1 (en) 2020-11-19
SG11202111778YA (en) 2021-12-30
JP2022533916A (ja) 2022-07-27
CN113748216A (zh) 2021-12-03

Similar Documents

Publication Publication Date Title
MX2020007904A (es) Llamado de pulso y base habilitado por maquina de aprendizaje para dispositivos de secuenciacion.
MX2021013983A (es) Metodo de secuenciacion monocanal basado en autoluminiscencia.
WO2018132635A8 (en) Methods for analyzing t cell receptors and b cell receptors
MX2019013993A (es) Amplificación de etiquetado extremo múltiple de ácidos nucleicos.
PH12019500181A1 (en) Information transmission method and information transmission apparatus
BR112018070903A2 (pt) métodos para detecção de câncer de pulmão
MX2019008347A (es) Secuenciamiento de canal bioptico, de una sola fuente de luz.
MX2021011433A (es) Sistema.
GEP20217218B (en) Method for authenticating security marking utilizing long afterglow emission, and security marking comprising one or more afterglow compound
EP3954458A4 (en) POLYMERASE CHAIN AMPLIFICATION DEVICE
MX2017013100A (es) Proceso para aislar y purificar ambrox.
ZA202007540B (en) Roadheader footage measurement method.
MX2019001214A (es) Metodos para el diagnostico de neoplasia de origen ginecologico.
GB2603386B (en) Selective dihydropyrrolopyrimidine JAK2 inhibitors
PH12019502511A1 (en) Short burst channel design and multiplexing
GB2586795B (en) Downhole retainer
SG11201811799VA (en) Primer set, kit and method for detecting two or more target nucleic acids
MX2017009952A (es) Metodo, aprato y sistema para ingresar caracteres.
NZ711109A (en) Polymerase chain reaction detection system
MX2021007222A (es) Factor i del complemento con optimizacion de codones.
EP4142072A4 (en) FLUORESCENT SYSTEM
SG10201706663RA (en) Primer Pair, Kit And Method For Detecting Babesia Gibsoni
MY186838A (en) Rapid detection of prawn viruses, using the multiplex real time pcr method and kit therefor
EP3824099A4 (en) MULTIPLEX NUCLEIC ACID DETECTION
MX2019001202A (es) Compuesto que inhibe la formacion de complejo de c-myc/max/adn.