CN113748216B - 一种基于自发光的单通道测序方法 - Google Patents
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- CN113748216B CN113748216B CN201980095781.8A CN201980095781A CN113748216B CN 113748216 B CN113748216 B CN 113748216B CN 201980095781 A CN201980095781 A CN 201980095781A CN 113748216 B CN113748216 B CN 113748216B
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Abstract
Description
first detection | second detection | |
A | 1 | 0 |
C | 1 | 1 |
G | 0 | 0 |
T | 0 | 1 |
底物1信号(W) | 底物2信号(W) | |
cycle1 | 0.1 | 210 |
cycle2 | 188 | 0.12 |
cycle3 | 173 | 169 |
cycle4 | 206 | 0.11 |
cycle5 | 198 | 0.13 |
cycle6 | 176 | 171 |
cycle7 | 0.13 | 186 |
cycle8 | 203 | 0.15 |
cycle9 | 166 | 159 |
cycle10 | 0.12 | 0.16 |
第一次检测(w) | 第二次检测(w) | |
cycle1 | 0.6 | 223 |
cycle2 | 312 | 0.7 |
cycle3 | 278 | 327 |
cycle4 | 254 | 0.3 |
cycle5 | 239 | 0.4 |
cycle6 | 243 | 275 |
cycle7 | 0.27 | 228 |
cycle8 | 267 | 0.5 |
cycle9 | 263 | 279 |
cycle10 | 0.7 | 0.6 |
Claims (13)
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PCT/CN2019/086974 WO2020227953A1 (zh) | 2019-05-15 | 2019-05-15 | 一种基于自发光的单通道测序方法 |
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CN113748216A CN113748216A (zh) | 2021-12-03 |
CN113748216B true CN113748216B (zh) | 2024-04-23 |
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US (1) | US20220205036A1 (zh) |
EP (1) | EP3988670A4 (zh) |
JP (1) | JP2022533916A (zh) |
KR (1) | KR20220009992A (zh) |
CN (1) | CN113748216B (zh) |
AU (1) | AU2019445584A1 (zh) |
BR (1) | BR112021022809A2 (zh) |
CA (1) | CA3139169A1 (zh) |
MX (1) | MX2021013983A (zh) |
SG (1) | SG11202111778YA (zh) |
WO (1) | WO2020227953A1 (zh) |
ZA (1) | ZA202110159B (zh) |
Families Citing this family (4)
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WO2021031109A1 (zh) * | 2019-08-20 | 2021-02-25 | 深圳华大智造极创科技有限公司 | 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法 |
CN115232867B (zh) * | 2022-07-21 | 2024-01-30 | 深圳赛陆医疗科技有限公司 | 一种快速的二代基因测序方法 |
WO2024067674A1 (zh) * | 2022-09-28 | 2024-04-04 | 深圳华大智造科技股份有限公司 | 测序方法 |
US11952624B1 (en) | 2022-11-07 | 2024-04-09 | GeneSense Technology Inc., Shanghai (CN) | Nucleic acid sequencing using self-luminescence |
Citations (4)
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WO2016040607A1 (en) * | 2014-09-12 | 2016-03-17 | Illumina, Inc. | Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence |
WO2018121587A1 (zh) * | 2016-12-27 | 2018-07-05 | 深圳华大生命科学研究院 | 一种基于单荧光染料的测序方法 |
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WO2019071474A1 (zh) * | 2017-10-11 | 2019-04-18 | 深圳华大智造科技有限公司 | 修饰的核苷或核苷酸 |
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WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
JP2002521064A (ja) | 1998-07-30 | 2002-07-16 | ソレックサ リミテッド | アレイ生体分子およびシークエンシングにおけるその使用 |
EP1337541B1 (en) | 2000-10-06 | 2007-03-07 | The Trustees of Columbia University in the City of New York | Massive parallel method for decoding DNA and RNA |
DK3587433T3 (da) | 2002-08-23 | 2020-05-18 | Illumina Cambridge Ltd | Modificerede nukleotider |
EP3673986A1 (en) | 2004-01-07 | 2020-07-01 | Illumina Cambridge Limited | Improvements in or relating to molecular arrays |
GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
CN1854308A (zh) * | 2005-04-21 | 2006-11-01 | 株式会社日立制作所 | 碱基序列检测方法 |
EP3981886A1 (en) * | 2011-09-23 | 2022-04-13 | Illumina, Inc. | Compositions for nucleic acid sequencing |
US9593373B2 (en) | 2013-03-15 | 2017-03-14 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
CN113321943A (zh) * | 2015-11-20 | 2021-08-31 | 加利福尼亚太平洋生物科学股份有限公司 | 标记的核苷酸类似物、反应混合物以及测序方法和系统 |
CN110418847A (zh) * | 2017-01-04 | 2019-11-05 | 考利达基因组股份有限公司 | 通过非标记的可逆终止子或天然核苷酸的分步测序 |
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2019
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- 2019-05-15 US US17/608,316 patent/US20220205036A1/en active Pending
- 2019-05-15 CN CN201980095781.8A patent/CN113748216B/zh active Active
- 2019-05-15 EP EP19928475.3A patent/EP3988670A4/en active Pending
- 2019-05-15 JP JP2021563644A patent/JP2022533916A/ja active Pending
- 2019-05-15 WO PCT/CN2019/086974 patent/WO2020227953A1/zh unknown
- 2019-05-15 MX MX2021013983A patent/MX2021013983A/es unknown
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- 2019-05-15 CA CA3139169A patent/CA3139169A1/en active Pending
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KR20220009992A (ko) | 2022-01-25 |
US20220205036A1 (en) | 2022-06-30 |
SG11202111778YA (en) | 2021-12-30 |
AU2019445584A1 (en) | 2022-01-20 |
ZA202110159B (en) | 2022-08-31 |
CN113748216A (zh) | 2021-12-03 |
EP3988670A1 (en) | 2022-04-27 |
JP2022533916A (ja) | 2022-07-27 |
MX2021013983A (es) | 2022-02-10 |
BR112021022809A2 (pt) | 2022-01-25 |
WO2020227953A1 (zh) | 2020-11-19 |
CA3139169A1 (en) | 2020-11-19 |
EP3988670A4 (en) | 2023-01-25 |
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