JP7485483B2 - 自家発光に基づく単一チャネルシーケンシング法 - Google Patents

自家発光に基づく単一チャネルシーケンシング法 Download PDF

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JP7485483B2
JP7485483B2 JP2021563644A JP2021563644A JP7485483B2 JP 7485483 B2 JP7485483 B2 JP 7485483B2 JP 2021563644 A JP2021563644 A JP 2021563644A JP 2021563644 A JP2021563644 A JP 2021563644A JP 7485483 B2 JP7485483 B2 JP 7485483B2
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luciferase
nucleic acid
molecular label
compound
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JP2022533916A5 (https=
JPWO2020227953A5 (https=
JP2022533916A (ja
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シャー リャオ,
シー チェン,
アオ チェン,
ウェンウェイ チャン,
チョンジュン スー,
ホンミン チェン,
ジエ ジャオ,
デフェン フー,
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Qingdao MGI Tech Co Ltd
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Qingdao MGI Tech Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021563644A 2019-05-15 2019-05-15 自家発光に基づく単一チャネルシーケンシング法 Active JP7485483B2 (ja)

Priority Applications (1)

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JP2024073905A JP7825660B2 (ja) 2019-05-15 2024-04-30 自家発光に基づく単一チャネルシーケンシング法

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PCT/CN2019/086974 WO2020227953A1 (zh) 2019-05-15 2019-05-15 一种基于自发光的单通道测序方法

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JP2022533916A JP2022533916A (ja) 2022-07-27
JP2022533916A5 JP2022533916A5 (https=) 2024-02-07
JPWO2020227953A5 JPWO2020227953A5 (https=) 2024-02-07
JP7485483B2 true JP7485483B2 (ja) 2024-05-16

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JP2024073905A Active JP7825660B2 (ja) 2019-05-15 2024-04-30 自家発光に基づく単一チャネルシーケンシング法

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US (1) US12565677B2 (https=)
EP (1) EP3988670B1 (https=)
JP (2) JP7485483B2 (https=)
KR (1) KR102843262B1 (https=)
CN (2) CN118931711A (https=)
AU (1) AU2019445584B2 (https=)
BR (1) BR112021022809A2 (https=)
CA (1) CA3139169A1 (https=)
MX (1) MX2021013983A (https=)
SG (1) SG11202111778YA (https=)
WO (1) WO2020227953A1 (https=)
ZA (1) ZA202110159B (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021031109A1 (zh) * 2019-08-20 2021-02-25 深圳华大智造极创科技有限公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
TW202342494A (zh) 2022-03-30 2023-11-01 日商肽夢想股份有限公司 具有TrkB結合活性之肽複合物
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
WO2024050815A1 (zh) 2022-09-09 2024-03-14 深圳华大生命科学研究院 杂芳环化合物在核酸检测中的用途
US20250297306A1 (en) 2022-09-28 2025-09-25 Mgi Tech Co., Ltd. Sequencing method
WO2024098185A1 (en) 2022-11-07 2024-05-16 GeneSense Technology Inc., Shanghai (CN) Nucleic acid sequencing using self-luminescence
WO2024108390A1 (zh) 2022-11-22 2024-05-30 深圳华大智造科技股份有限公司 基因测序芯片、封装结构、系统及清洗、制作、测序方法

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20130079232A1 (en) 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2016040607A1 (en) 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
WO2018121587A1 (zh) 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法

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CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
JP2004510433A (ja) 2000-10-06 2004-04-08 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク Dnaおよびrnaを解読するための大量並行方法
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
CN1854308A (zh) 2005-04-21 2006-11-01 株式会社日立制作所 碱基序列检测方法
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
WO2017087823A1 (en) * 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
EP3376997B1 (en) 2015-11-20 2024-11-13 Pacific Biosciences of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
US10851410B2 (en) 2017-01-04 2020-12-01 Mgi Tech Co., Ltd. Nucleic acid sequencing using affinity reagents
BR112019014683A2 (pt) 2017-03-07 2020-02-18 Illumina, Inc. Sequenciamento com dois canais óticos, com fonte de luz única
CN110650968B (zh) * 2017-10-11 2022-07-05 深圳华大智造科技股份有限公司 修饰的核苷或核苷酸

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US20130079232A1 (en) 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2016040607A1 (en) 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
WO2018121587A1 (zh) 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法

Also Published As

Publication number Publication date
CN113748216B (zh) 2024-04-23
ZA202110159B (en) 2022-08-31
CN113748216A (zh) 2021-12-03
KR102843262B1 (ko) 2025-08-06
KR20220009992A (ko) 2022-01-25
EP3988670A1 (en) 2022-04-27
AU2019445584B2 (en) 2024-09-05
EP3988670B1 (en) 2026-04-22
MX2021013983A (es) 2022-02-10
WO2020227953A1 (zh) 2020-11-19
EP3988670A4 (en) 2023-01-25
CN118931711A (zh) 2024-11-12
CA3139169A1 (en) 2020-11-19
BR112021022809A2 (pt) 2022-01-25
SG11202111778YA (en) 2021-12-30
AU2019445584A1 (en) 2022-01-20
JP7825660B2 (ja) 2026-03-06
US12565677B2 (en) 2026-03-03
US20220205036A1 (en) 2022-06-30
JP2022533916A (ja) 2022-07-27
JP2024099789A (ja) 2024-07-25

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