KR102637453B1 - Cosmetic materials for improving acnegenic skin, Manufacturing method thereof and Cosmetics containing the same - Google Patents

Abstract

The present invention relates to cosmetics containing natural herbal medicines, methods for producing the same, and cosmetics using the same. To be more specific, the present invention relates to acne treatment derived from natural plant ingredients using fermented extracts of seven types of natural herbal medicines such as Hwangchil tree. It relates to cosmetics with excellent skin improvement effects, their manufacturing methods, and functional cosmetics using them.

Description

Cosmetic materials for improving acnegenic skin, Manufacturing method thereof and Cosmetics containing the same}

The present invention relates to a cosmetic with an excellent acne-improving effect derived from natural plant ingredients using natural herbal medicine, a method for producing the same, and a cosmetic with an acne-improving effect using the same.

Acne is an inflammatory skin disease that causes comedones, papules, pus blisters, nodules, and pseudocysts to occur mainly on the face, neck, chest, back, and shoulders. Acne usually occurs in the early teens, but symptoms can worsen around the 20s and can also occur in adults in their 30s and 40s. Even without treatment, it usually disappears after several years, but if left untreated, it can leave permanent scars, which becomes a cosmetic problem. Although it is not a life-threatening disease, it places a psychological burden on the patient.

The exact cause of acne is not yet clear, but it is known that several causes work together. Male hormones increased during puberty cause the sebaceous glands of the skin (skin oil glands) to enlarge, and these sebaceous glands produce an oily substance called sebum. When this sebum secretion increases, the sebum stimulates the inner wall of the hair follicle, causing the inner wall cells to grow more. It causes them to fall out quickly, and these shed cells clump together and block the pores of the hair follicles, forming comedones (sebum that accumulates and hardens in the hair follicles), which is the basic lesion of acne. In addition, lipolytic enzymes secreted by bacteria residing in hair follicles break down neutral fats in sebum to form free fatty acids. The formed free fatty acids stimulate the hair follicles again to burst the hair follicle walls, allowing sebum, bacteria, and shed cells to enter the skin. It is released and causes erythema, edema, and pus.

In order to treat acne, consistent management is required, and natural ingredients are needed that are safe and have few side effects even if taken or used for a long time and are effective in treating acne. As such, many researches and products are being developed on various acne improvement and/or prevention ingredients derived from natural materials, and cosmetics with acne improvement effects that can be applied to various application products while minimizing side effects such as skin troubles are being developed. The demand for it is increasing.

Korean Patent Registration No. 10-0870420 (announcement date 2008.11.19.) Korean Patent Registration No. 10-1190943 (announcement date 2012.10.08)

As a result of the present invention's efforts to develop a cosmetic that has an acne-improving effect while preventing and minimizing skin side effects, the present invention was completed by discovering an acne-improving cosmetic material with an optimal composition and composition ratio and its composition ratio. In other words, the purpose of the present invention is to provide an acne-improving cosmetic, a method for manufacturing the same, and cosmetics using the same.

In order to solve the above problems, the acne improving cosmetic of the present invention includes distilled extract of fermented complex herbal medicine; and a complex herbal medicine fermentation product; the complex herbal medicine includes Dendropanax Morbifera, Eriobotrya Japonica Leaf, Adenophora Stricta Leaf & Root, Hordeum Vulgare, Includes Saururus Chinensis, Artemisia Annua seed and Tea Tree Leaf.

In addition, another object of the present invention is a method of manufacturing the acne-improving cosmetic, after ultrasonically washing a mixture of Hwangchil tree, loquat tree leaves, Dangjangi leaves and roots, barley sprouts, Trillium chinensis, Artemisia seeds and tea tree leaves. , the first step of preparing the dried composite herbal medicine; Step 2 of producing a distilled extract and a fermented complex herbal medicine, respectively, using the complex herbal medicine; and step 3 of mixing the distilled extract of the complex herbal medicine fermentation product and the complex herbal medicine fermentation product to prepare a mixed solution.

In addition, the purpose of the present invention is to provide cosmetics with excellent acne improvement effects using the above cosmetics.

The cosmetic of the present invention has excellent skin soothing and moisturizing effects, does not cause skin troubles, and is used as skin cosmetics in various formulations with excellent skin whitening, wrinkle improvement, and acne improvement effects, and scalp and hair cosmetics such as shampoo and conditioner. It can be used as a cosmetic.

Figures 1A to 1D show the results of the safety evaluation on human skin conducted in Experimental Example 1.
Figures 2 and 3 show the results of measuring whether cosmetics contain insecticidal ingredients.

Hereinafter, a method for producing the acne-improving cosmetic of the present invention will be described in more detail.

The acne-improving cosmetic of the present invention is prepared by ultrasonically washing a mixture of Hwangchil tree, loquat tree leaves, Dangjangi leaves and roots, barley sprouts, trillium chinensis, artemisia seeds, and tea tree leaves, and then drying them to prepare the dried composite herbal medicine. step; Step 2 of producing a distilled extract and a fermented complex herbal medicine, respectively, using the complex herbal medicine; And step 3 of mixing the distilled extract of the complex herbal medicine fermentation product and the complex herbal medicine fermentation product to prepare a mixed solution.

The mixture in step 1 includes 10 to 20% by weight of Eriobotrya Japonica Leaf, 10 to 20% by weight of Adenophora Stricta Leaf & Root, 10 to 20% by weight of Hordeum Vulgare, Contains 5 to 15% by weight of Saururus Chinensis, 5 to 15% by weight of Artemisia Annua seeds, 3 to 7% by weight of Tea Tree Leaf, and the remaining amount of Dendropanax Morbifera. It can be done, preferably 12 to 18% by weight of loquat leaves, 14 to 20% by weight of dandelion leaves and roots, 12 to 18% by weight of sprouted barley, 5 to 12% by weight of Trifolium primrose, and 8 to 14% by weight of mugwort seeds. , it may include 3.5 to 7.0% by weight of tea tree leaves and the remaining amount of Hwangchil tree, more preferably 12 to 16% by weight of loquat leaves, 14 to 19% by weight of Danjangi leaves and roots, and 13 to 18% by weight of sprouted barley. % by weight, it may include 7 to 12% by weight of Trifolium primrose, 8.5 to 12.5% by weight of mugwort seeds, 4.0 to 6.5% by weight of tea tree leaves, and the remaining amount of Hwangchil tree.

And, the Hwangchil tree includes a mixture of leaves, stems and fruits of the Hwangchil tree.

The loquat tree leaves have the effect of relieving heat such as heat rash due to the cold nature of the loquat when applied to the affected area, and contain vitamin C, which is good for skin care. In addition, it is rich in potassium and various minerals, so it is used for the effect of clearing the skin and maintaining healthy skin to prevent skin troubles. If loquat tree leaves are less than 10% by weight of the total weight of the mixture, loquat There may be a problem of the tree leaves not being effective, and if used in excess of 20% by weight, there may be a problem of skin irritation due to the cold nature of the loquat tree leaves.

In addition, the leaves and roots of the Danjangi are used to prevent tissue damage due to inflammation and to provide a defense response to biological tissue against various infectious agents, and the content of the leaves and roots of the Danjangi is less than 10% by weight of the total weight of the mixture. Since it does not contain active ingredients, there may be problems with the efficacy of preventing or treating inflammatory diseases, such as tissue damage and impaired defense responses from external stimuli, being significantly reduced. If used in excess of 20% by weight, there is no harm as it is not toxic, but excessive use may result. If used, there may be a problem of the disease progressing to major pathologies (hypersensitivity allergic disease, chronic inflammatory disease).

In addition, the sprout barley is used for the effect of alleviating skin troubles and controlling sebum. If the sprout barley content is less than 10% by weight of the total weight of the mixture, there may be a problem in alleviating skin troubles, and it is used in excess of 20% by weight. If so, there may be a problem that the fermentation by microorganisms does not work well during the fermentation of the hot water extract.

In addition, if the amount of ginseng in the total weight of the mixture is less than 5% by weight, the effect of cosmetics in alleviating skin troubles may be reduced, and if it exceeds 15% by weight, fermentation by microorganisms does not work well during the fermentation of the hot water extract, and skin cell toxicity problems may occur. may occur.

In addition, artemisia seeds contain a large amount of flavonoids, which prevent cell damage by acting as an antioxidant and are used for anti-inflammatory effects on the skin. If the content of artemisia seeds is less than 5% by weight of the total weight, the amount used is If this amount is small, the antioxidant effect and skin fungus suppressing effect may be reduced, and if it exceeds 15% by weight, the cold nature may interfere with blood circulation in the skin, which may worsen problems such as rashes, inflammatory acne, and skin diseases.

In addition, the tea tree leaves are used for the sterilizing effect and the effect of controlling the amount of excessive sebum secretion. If the tea tree leaf content of the total weight of the mixture is less than 3% by weight, there is a problem that the effect is insufficient for acne caused by Demodex. , It is uneconomical to use more than 7% by weight, and it may actually cause skin problems, so it is better to use it within the above range.

In addition, the Hwangchil tree belongs to the Araliaceae family and is an evergreen broad-leaved tree that grows only on the southwestern coast of Korea and Jeju Island in the world. In the herb class, Hwangchil tree is recorded to be effective in removing heat and treating burns and is harmless. In addition, Hwangchil tree is also called ginseng tree because it contains a lot of saponin components like ginseng. Hwangchil tree is used for skin whitening effect by preventing skin aging and melanin pigmentation due to its excellent antioxidant activity. Among the total weight of the mixture, the leaves and roots of loquat tree, daffodils leaves and roots, barley sprouts, ginseng herb, and artemisia seeds as described above are included in the total weight of the mixture. In addition to tea tree leaves, it can be used as a weight percent of the remaining amount out of 100 weight percent.

In the manufacturing method of the present invention, the ultrasonic cleaning in step 1 can be performed using a general ultrasonic cleaning device used in the industry, and is not particularly limited, and heavy metals and residual pesticides, etc. are completely removed through ultrasonic cleaning. Perform.

Next, the second step is a process of producing a fermented product and a distilled extract of the fermented product using the complex herbal medicine of the first step.

[Distilled extract of fermented complex herbal medicine]

First, the distilled extract of the fermented complex herbal medicine (hereinafter referred to as ‘distilled extract’) can be produced by performing the following process.

The distilled extract of the fermented composite herbal medicine is obtained through a step 2-1 of steaming the composite herbal medicine; Step 2-2 of mixing the steamed composite herbal medicine and water, followed by heat treatment; Steps 2-3 of filtering the heat-treated product and then cooling it to obtain a hot water extract; Steps 2-4 of mixing the hot water extract with a fermentation agent containing makgeolli yeast and reishi mushroom mycelium or Sanghwang mushroom mycelium, fermenting it, and then filtering to obtain a fermented product; and steps 2-4 of performing a vacuum distillation extraction process on the fermented product to obtain a distilled extract and then filtering the fermented product to obtain a filtrate.

In the distillation extract manufacturing process, steaming in step 2-1 can be performed by applying steam at 80 to 90°C to the composite herbal medicine for 2 to 4 hours. At this time, steaming treatment can be performed by dividing hard herbal medicines such as roots and stems of complex herbal medicines and soft herbal medicines such as leaves and fruits. For example, hard herbal medicines such as roots and stems can be steamed under 85 ~ 90℃. Steaming is performed for 3 to 4 hours, and soft herbal medicines such as leaves and fruits are steamed at 80 to 85°C for 2 to 3 hours, and then mixed to perform a 2-2 heat treatment process. .

In the distillation extract manufacturing process, the heat treatment in step 2-2 is performed by mixing the steamed composite herbal medicine and water at a weight ratio of 1:2.5 to 5.0, preferably 1:2.8 to 5.0, and then heating the mixture at 85 to 95°C. for 10 to 14 hours under low temperature, preferably at 88 to 92°C for 12 to 14 hours. At this time, if the mixing amount of water is less than 2.5 weight ratio, there may be a problem that the amount of hot water extract is too small, and if it exceeds 5.0 weight ratio, the concentration of active ingredients beneficial to the skin in the hot water extract is too low and fermentation may not proceed well in the future, so water must be kept within the above range. It is recommended to perform heat treatment by mixing with .

In the distillation extract manufacturing process, step 2-3 is a process of obtaining a hot water extract by filtering and cooling the heat-treated product obtained by performing the heat treatment in step 2-2. At this time, the filtration method can be performed by a general method. , it must not contain solid matter such as foreign substances or residues in the hot water extract.

In the distillation extract manufacturing process, steps 2-4 are a process of obtaining a fermented product obtained by fermenting the hot water extract obtained in steps 2-3, and preferably 2.0 to 5.0 parts by weight of a fermentation agent based on 100 parts by weight of the hot water extract. After mixing 2.2 to 4.0 parts by weight of fermentation agent, fermentation was performed at 32 to 40°C, preferably at 36 to 40°C, for 45 to 60 hours, preferably 46 to 52 hours, and then filtered to obtain a fermented product. It is a process. At this time, if the amount of the fermentation agent used is less than 2.0 parts by weight, there may be a problem that fermentation does not proceed well, and even if the amount of fermentation agent used exceeds 5.0 parts by weight, the speed of fermentation does not become faster, and the amount of fermented product obtained does not increase. Rather, cosmetics using the obtained fermented product may cause skin problems, so it is better to use the fermentation agent within the above range. In addition, if the temperature during fermentation is less than 32℃ or the fermentation time is less than 45 hours, fermentation does not proceed or the fermentation is insufficient. If the fermentation temperature exceeds 40℃ or the fermentation time exceeds 60 hours, the fermentation proceeds too much. Since there may be a problem that the effect of cosmetics using fermented products is reduced, it is recommended to perform fermentation within the above fermentation temperature and time range.

In addition, the fermentation agent is preferably comprised of makgeolli yeast, reishi mushroom mycelium, and mycelium of Sanghwang mushroom in a weight ratio of 1.5 to 3.0:1, preferably in a weight ratio of 1.6 to 2.8:1, and more preferably in a weight ratio of 1.8 to 2.5:1.

In addition, the makgeolli yeast can be a yeast used in general makgeolli production, and preferably yeast enzyme bacteria can be used.

In addition, based on 100 parts by weight of the hot water extract, 0.30 to 1.00 parts by weight of sugar, 0.05 to 0.35 parts by weight of salt, and 0.10 to 0.50 parts by weight of persimmon vinegar, preferably 0.40 to 0.90 parts by weight of sugar, 0.10 to 0.30 parts by weight of salt, and persimmon vinegar. Fermentation can be performed by adding 0.20 to 0.40 parts by weight to obtain the fermented product.

Next, in steps 2-4, the fermented product of steps 2-3 is subjected to a vacuum distillation extraction process to obtain a distilled extract, and then filtered to obtain a filtrate, thereby producing a distilled extract of the fermented complex herbal medicine, which is a cosmetic ingredient. It's fair.

The vacuum distillation extraction process can be carried out by vacuum distillation extraction, which is an extraction method that collects only the dew that forms by vaporizing the fermented product while heating it at 80 to 85 ° C.

[Compound herbal medicine fermentation product]

The fermented complex herbal medicine, which is one of the cosmetic compositions of the present invention, is a fermented product prepared under different hot water extraction conditions, unlike the distilled extract described above, without performing a vacuum distillation extraction process.

To be more specific, the fermented composite herbal medicine is prepared by mixing the composite herbal medicine and water, followed by low-temperature heat treatment in step 2-1; Step 2-2 of filtering the low-temperature heat-treated product and then cooling it to obtain a low-temperature hot water extract; Steps 2-3 of mixing the low-temperature hot water extract with a fermentation agent containing makgeolli yeast and reishi mushroom mycelium or mycelium of Sanghwang mushroom, fermenting it, and then filtering to obtain a fermented product; and steps 2-4 of heat-treating the obtained fermented product to kill it and then obtaining a filtered filtrate.

In the manufacturing process of fermented composite herbal medicine, the low-temperature heat treatment in step 2-1 is performed by mixing the composite herbal medicine and water at a weight ratio of 1:3.0 to 6.0, preferably 1:3.5 to 5.0, and then mixing the composite herbal medicine and water in a weight ratio of 40 to 60. It is performed at ℃ for 16 to 24 hours, preferably at 50 to 62℃ for 16 to 20 hours. At this time, if the mixing amount of water is less than 3 weight ratio, there may be a problem that the amount of low-temperature hot water extract is too small, and if it exceeds 6 weight ratio, the concentration of active ingredients beneficial to the skin in the low-temperature hot water extract is too low, and fermentation may not proceed well in the future, so it should be within the above range. It is recommended to perform low-temperature heat treatment by mixing it with water.

In the manufacturing process of fermented complex herbal medicine, step 2-2 is a process of obtaining a low-temperature hot water extract by filtering and cooling the low-temperature heat-treated product obtained by performing the low-temperature heat treatment in step 2-1. In this case, the filtration method is a general method. It can be performed as long as it does not contain solid substances such as foreign substances and residues in the filtered low-temperature hydrothermal extract.

In the process of manufacturing a fermented product of a complex herbal medicine, steps 2-3 are a process of obtaining a fermented product obtained by fermenting the low-temperature hot water extract obtained in step 2-2, and 2.0 to 5.0 parts by weight of fermentation agent is added to 100 parts by weight of the hot water extract. , preferably after mixing 2.2 to 4.0 parts by weight of fermentation agent, fermented at 32 to 40°C, preferably at 36 to 40°C for 45 to 60 hours, preferably 46 to 52 hours, then filtered and fermented. This is the process of obtaining water. At this time, if the amount of the fermentation agent used is less than 2.0 parts by weight, there may be a problem that fermentation does not proceed well, and even if the amount of fermentation agent used exceeds 5.0 parts by weight, the speed of fermentation does not become faster, and the amount of fermented product obtained does not increase. Rather, cosmetics using the obtained fermented product may cause skin problems, so it is better to use the fermentation agent within the above range. In addition, if the temperature during fermentation is less than 32℃ or the fermentation time is less than 45 hours, fermentation does not proceed or the fermentation is insufficient. If the fermentation temperature exceeds 40℃ or the fermentation time exceeds 60 hours, the fermentation proceeds too much. Since there may be a problem that the effect of cosmetics using fermented products is reduced, it is recommended to perform fermentation within the above fermentation temperature and time range.

In addition, the fermentation agent is preferably comprised of makgeolli yeast and reishi mushroom mycelium or mycelium Sanghwang mushroom in a weight ratio of 1.5 to 3.0:1, preferably in a weight ratio of 1.6 to 2.8:1, and more preferably in a weight ratio of 1.8 to 2.5:1.

The makgeolli yeast and/or reishi mushroom mycelium or Sanghwang mushroom mycelium are the same as the makgeolli yeast, reishi mushroom mycelium, or Sanghwang mushroom mycelium used in the fermentation process of the distilled extract manufacturing process described above.

In addition, based on 100 parts by weight of the hot water extract, 0.30 to 1.00 parts by weight of sugar, 0.05 to 0.35 parts by weight of salt, and 0.10 to 0.50 parts by weight of persimmon vinegar, preferably 0.40 to 0.90 parts by weight of sugar, 0.10 to 0.30 parts by weight of salt, and persimmon vinegar. Fermented product can be obtained by performing fermentation by adding 0.20 to 0.40 parts by weight.

Next, in the process for producing a fermented compound herbal medicine, steps 2-4 are a process of obtaining a fermented compound herbal medicine by vacuum heat treating the obtained fermented product to obtain an extract and then filtering the obtained extract to obtain a filtrate. . At this time, vacuum heat treatment can be performed by vacuum heating the obtained fermented product at 82 to 95°C, preferably 85 to 90°C.

In the cosmetic manufacturing process of the present invention, step 3 is a process of producing a cosmetic by mixing the distilled extract of the complex herbal medicine fermentation product and the complex herbal medicine fermentation product prepared by the method described above.

The mixed solution can be appropriately mixed and used depending on the type of cosmetic to be manufactured using the cosmetic of the present invention. Preferably, the mixed solution contains 10 to 40% by weight of the composite herbal medicine fermentation product and the remaining amount of the composite herbal medicine fermentation product. It can be used by adjusting it within the range including distilled extract.

When the cosmetic of the present invention is used as a cosmetic for cream-type cosmetics, 10 to 20% by weight of the complex herbal medicine fermentation and the remaining amount of the distilled extract of the complex herbal medicine fermentation, preferably 10 to 15% by weight of the complex herbal medicine fermentation and It is recommended to use the remaining amount of the distilled extract of the fermented complex herbal medicine mixed with it.

A preferred example of cream-type cosmetics is shown in Table 1 below.

division type Content (% by weight) skin conditioning agent glycerin 6.0 ~ 8.2 skin conditioning agent Caprylic/capric triride glyceride 5.0 ~ 8.0 antioxidant Sunflower seed oil, tocopherol 3.0 ~ 6.5 whitener Niacinamide 1.0 ~ 3.0 Moisturizing and anti-inflammatory pantherol 1.0 ~ 3.0 Skin soothing and skin barrier strengthening agent Centella asiatica extract 0.1 ~ 2.0 moisturizer Low molecular weight hyaluronic acid 0.5 ~ 3.0 Skin immunity enhancement and skin inflammation reliever Lactobacillus fermentation lysate 0.1 ~ 2.0 Wrinkle improvement agent peptide 0.1 ~ 2.0 Wrinkle improvement agent Adenosine 0.01 ~ 0.15 Atopic skin improvement cosmetic 10 to 20% by weight of fermented complex herbal medicine and 80 to 90% by weight of distilled extract remaining balance
100% by weight

In addition, when the cosmetic of the present invention is used as a cosmetic for skin type cosmetics, 20 to 40% by weight of the fermented complex herbal medicine and the remaining amount of the distilled extract of the fermented complex herbal medicine are used, preferably 20 to 35% by weight of the fermented complex herbal medicine. It is recommended to use a mixture of % and the remaining amount of distilled extract of the fermented complex herbal medicine.

A preferred example of skin type cosmetics is shown in Table 2 below.

division type Content (% by weight) moisturizer Dipropylene glycol 10 to 14 moisturizer Low molecular weight hyaluronic acid 1.0 ~ 4.0 Skin soothing and skin barrier strengthening agent Centella asiatica extract (powder) 3.0 ~ 7.0 Skin soothing and skin barrier strengthening agent Dandelion extract 1.5 ~ 5.0 Skin immunity enhancement and skin inflammation reliever Lactobacillus fermentation lysate 1.0 ~ 3.0 whitener Niacinamide 0.5 ~ 4.0 Wrinkle improvement agent peptide 0.1 ~ 2.0 Wrinkle improvement agent Adenosine 0.01 ~ 0.15 skin regeneration agent Madeca Society 0.01 ~ 0.15 Atopic skin improvement cosmetic 20 to 40% by weight of fermented complex herbal medicine and 60 to 80% by weight of distilled extract remaining balance
100% by weight

The acne-improving cosmetic of the present invention prepared by the method described above is available in various formulations such as bars, skins, and lotions that do not cause skin trouble, have excellent soothing and moisturizing effects, have skin whitening and wrinkle-improving effects, and have excellent acne-improving effects. It is suitable for use as a cosmetic ingredient in functional cosmetics for skin.

Hereinafter, the present invention will be described in more detail through examples. However, the following examples do not limit the scope of the present invention, and should be interpreted to aid understanding of the present invention.

[Example]

Preparation Example 1-1: Preparation of distilled extract of fermented complex herbal medicine

A mixture of 15% by weight of loquat leaves, 15% by weight of Tangerine leaves and roots, 15% by weight of sprouted barley, 10% by weight of Trifolium primrose, 10% by weight of Artemisia seeds, 5% by weight of tea tree leaves and the remaining amount of Hwangchil tree. The mixture was ultrasonic washed in an ultrasonic device and then dried to prepare a composite herbal medicine. And, the Hwangchil tree includes a mixture of leaves, stems and fruits of the Hwangchil tree.

Among the complex herbal medicines, after dividing them into hard herbal medicines such as roots and stems and soft herbal medicines such as leaves and fruits, the hard herbal medicines were steamed at 87 to 90°C for about 4 hours. In addition, the herbal medicine in the soft area was steamed at 80 to 83°C for about 2 hours. Afterwards, the steamed complex herbal medicine was prepared by mixing the steamed hard part and soft part herbal medicine.

Next, the steamed composite herbal medicine mixed with water at a weight ratio of 1:3.1 was subjected to a hydrothermal process (heat treatment) at 90°C for 12 hours, followed by filtration and cooling to obtain a hydrothermal extract.

Next, about 4 kg of hot water extract was added to the fermentation tank, and then a fermenting agent containing makgeolli yeast, yeast enzyme bacteria, and reishi mushroom mycelium at a weight ratio of 2:1, sugar, salt, and persimmon vinegar were added to the fermentation tank. At this time, the amount of fermentation agent used was 2.55 parts by weight based on 100 parts by weight of the hot water extract added to the fermenter. Additionally, with respect to 100 parts by weight of the hot water extract, 0.60 parts by weight of sugar, 0.150 parts by weight of salt, and 0.260 parts by weight of persimmon vinegar were added.

Then, the fermentation process was performed at 38°C for 52 hours and then filtered to obtain a fermented product.

Next, the fermented product was vacuum heated to 90°C, a vacuum distillation extraction process was performed to obtain the obtained distillate, and then filtered to prepare a distilled extract of the complex herbal medicine fermentation, and the prepared distilled extract was Stored refrigerated (2 ~ 3℃).

Preparation Examples 1-2 to 1-3 and Comparative Preparation Examples 1-1 to 1-5

A distilled extract of the fermented composite herbal medicine was prepared in the same manner as in Preparation Example 1-1, but the content of the herbal medicine in the composite herbal medicine was varied or the fermentation conditions were changed as shown in Table 3 below to prepare the distilled extract, respectively, as shown in Preparation Example 1-2. ~1-3 and Comparative Preparation Examples 1-1 to 1-5 were carried out, respectively.

division Preparation example
1-1 Preparation example
1-2 Preparation example
1-3 comparison
Preparation example
1-1 comparison
Preparation example
1-2 comparison
Preparation example
1-3 comparison
Preparation example
1-4 comparison
Preparation example
1-5 complex
herbal medicine
(weight%) loquat tree leaves 15 18 12 5 15 18 15 15 Dangjandae leaves and roots 15 12 18 15 5 18 15 15 sprout barley 15 11 18 15 15 7 10 10 300 seconds 10 12 7 10 10 3 10 10 Artemisia seeds 10 13 8 10 10 4 10 10 tea tree leaves 5 5 5 5 5 7 5 5 Hwangchil tree Remaining amount 100% by weight Fermentation agent (gulli yeast and reishi mushroom mycelium weight ratio) 1:3 1:3 1:3 1:3 1:3 1:3 1:1 1:5

Preparation Example 2: Preparation of fermented complex herbal medicine

A composite herbal medicine was prepared by mixing, washing and drying herbal medicines with the same composition and content as in Preparation Example 1-1.

Next, the mixed herbal medicine mixed with water at a weight ratio of 1:4.5 was subjected to a hydrothermal process (heat treatment) at 50°C for 18 hours, followed by filtration and cooling to obtain a low-temperature hydrothermal extract.

Next, about 4 kg of hot water extract was added to the fermentation tank, and then a fermenting agent containing makgeolli yeast, yeast enzyme bacteria, and reishi mushroom mycelium at a weight ratio of 2:1, sugar, salt, and persimmon vinegar were added to the fermentation tank. At this time, the amount of fermentation agent used was 2.56 parts by weight based on 100 parts by weight of the hot water extract added to the fermenter. Additionally, with respect to 100 parts by weight of the hot water extract, 0.62 parts by weight of sugar, 0.145 parts by weight of salt, and 0.252 parts by weight of persimmon vinegar were added.

Then, the fermentation process was performed at 38°C for 48 hours, then filtered to obtain a fermented product, which was stored in refrigeration (2 to 3°C).

Example 1: Preparation of acne improving cosmetics

A cosmetic was prepared by mixing 30% by weight of the fermented complex herbal medicine prepared in Preparation Example 2 and 70% by weight of the distilled extract from Preparation Example 1-1 in a homogenizer.

Examples 2 to 5 and Comparative Examples 1 to 5

Cosmetics were prepared in the same manner as in Example 1, but with different fermented herbal medicines and distilled extracts as shown in Table 4 below, and Examples 2 to 5 and Comparative Examples 1 to 5 were performed, respectively.

division Fermented complex herbal medicine
(Preparation Example 2) Content Fermented complex herbal medicine
distilled extract Example 1 30% by weight Preparation example 1-1 70% by weight Example 2 30% by weight Preparation example 1-2 70% by weight Example 3 30% by weight Preparation example 1-3 70% by weight Example 4 12% by weight Preparation example 1-1 88% by weight Example 5 38% by weight Preparation example 1-1 62% by weight Comparative Example 1 30% by weight Comparison preparation example 1-1 70% by weight Comparative Example 2 30% by weight Comparison preparation example 1-2 70% by weight Comparative Example 3 30% by weight Comparison preparation example 1-3 70% by weight Comparative Example 4 30% by weight Comparison preparation example 1-4 70% by weight Comparative Example 5 30% by weight Comparison Preparation Example 1-5 70% by weight Comparative Example 6 5% by weight Preparation example 1-1 95% by weight Comparative Example 7 50% by weight Comparison preparation example 1-4 50% by weight

Experimental Example 1: Skin cytotoxicity experiment

RAW 264.7 cells (Korea Cell Line Bank) were suspended at a concentration of 2×10 ⁵ cells/mL in DMEM (Gibco) medium supplemented with 10% fetal bovine serum and plated in 96-well plates (200 ㎕ each). plate) and attached. Afterwards, the cosmetics prepared in Examples 1 to 5 and Comparative Examples 1 to 7 were each treated at a concentration of 1000 μg/ml and cultured for 20 hours. 20 μl of 5 mg/ml MTT solution was added per well and cultured for an additional 4 hours. After removing the supernatant, 100 μl of DMSO was added, and the absorbance was measured at 570 nm. Cell viability was calculated according to Equation 1 below, taking the negative control group treated with PBS as 100%, and the results are shown in Table 5 below.

[Equation 1]

Cell viability (%) = (cosmetic treatment group absorbance value/negative control group absorbance value) x 100%

division Cell survival rate (%) Example 1 100.0 Example 2 104.3 Example 3 102.6 Example 4 108.4 Example 5 105.4 Comparative Example 1 99.4 Comparative Example 2 103.0 Comparative Example 3 105.0 Comparative Example 4 99.8 Comparative Example 5 110.5 Comparative Example 6 96.9 Comparative Example 7 107.5

Looking at the cell viability measurement results in Table 5 above, overall, both Examples and Comparative Examples showed no cytotoxicity to skin cells. However, Comparative Example 3 using the cosmetics of Comparative Preparation Examples 1-5 in which makgeolli yeast and reishi mushroom mycelium were used as fermentation agents at a weight ratio of 1:5, and Comparative Example 3 using cosmetics using 50% by weight, exceeding 40% by weight, of fermented complex herbal medicine. In the case of Example 7, the skin cell survival rate was somewhat low, which is considered to be relatively more toxic to the skin than other cosmetics.

Experimental Example 2: Experiment on skin whitening effect through tyrosinase inhibition effect

In order to analyze the tyrosinase inhibitory effect of the cosmetics prepared in the above Examples and Comparative Examples, mushroom-derived tyrosinase (Sigma) was used to determine whether the cosmetics were able to convert L L-tyrosine into L-DOPA (L-3,4- The efficacy of inhibiting conversion to dihydroxy phenylalanine was analyzed.

For the tyrosinase inhibition assay for L-tyrosine, 120 μl 0.1M sodium phosphate buffer solution (pH 6.5), 20 μl 1,000 unit/mL tyrosinase, and 20 μl sample were each added to a 96-well microplate and incubated at room temperature for 10 minutes. After the reaction, 40 μl of 1.5mM L-tyrosine was added, and the absorbance was measured at 490 nm for 10 minutes at 25°C.

Distilled water was used as the negative control group (N-control), kojic acid (KA, Sigma) was used as the positive control group (P-control), and the experimental group was analyzed for cosmetic content by concentration of 20, 40, 80, and 100 μg/ml, and the measured IC The value of ₅₀ (half maximal inhibitory concentration) is shown in Table 6 below.

division Tyrosinase inhibition ability (IC ₅₀ =mg/ml) positive control 49.30 Example 1 61.72 Example 2 65.31 Example 3 56.40 Example 4 66.91 Example 5 52.68 Comparative Example 1 71.53 Comparative Example 2 73.18 Comparative Example 3 69.07 Comparative Example 4 75.31 Comparative Example 5 63.93 Comparative Example 6 80.42 Comparative Example 7 58.02

Looking at the tyrosinase inhibition activity measurement results in Table 6 above, it was confirmed that Examples 1 to 5 had an IC ₅₀ at a concentration of 68 mg/ml or less.

On the other hand, Comparative Examples 1 to 5 had IC ₅₀ at relatively high concentrations compared to Examples 1 to 5, and showed a relatively low tyrosinase inhibitory effect compared to the Examples.

In addition, in the case of Comparative Example 5, in which reishi mushroom mycelium was used at a weight ratio of 5 as a fermenting agent, the tyrosinase inhibitory effect was relatively poor compared to other examples.

In addition, in the case of Comparative Example 6 (5 wt%), in which less than 10 wt% of the herbal medicine complex in the cosmetic was used, there was a problem that the tyrosinase inhibitory effect was significantly insufficient.

In the case of Comparative Example 7 (50% by weight), which is a cosmetic using 50% by weight of the fermented herbal medicine exceeding 40% by weight, the tyrosinase inhibitory effect was somewhat reduced compared to Example 5, and in Experimental Example 1, the skin As there was some cytotoxicity, it was concluded that using more than 40% by weight of the fermented complex herbal medicine actually reduces the effectiveness of the cosmetic due to excessive use.

Through this, it was confirmed that the cosmetic of the present invention can have a whitening effect through a tyrosinase inhibitory effect.

Experimental Example 3: Measurement of skin wrinkle improvement effect

In order to confirm the skin wrinkle improvement effect of the cosmetics prepared in the above Examples and Comparative Examples, the inhibition of elastase activity was measured in the following manner, and the results are shown in Table 7 below.

The measurement method was to dispense 100㎕ of 100mM Tris-HCl buffer (pH 8.0) into a 96-well plate, add the above cosmetics in equal amounts, and then dispense 20㎕ of 2.9mM N-Succinyl-(Ala)3-pnitroanilide. Next, 15 ㎕ of elastase enzyme solution prepared at 0.2 units was added, mixed, and reacted at 25°C for 20 minutes. The reaction was terminated by leaving the reaction product on ice for 5 minutes, the absorbance was measured using a microplate reader with a wavelength of 410 nm, and the elastase activity rate was measured by substituting it into Equation 2 below, and the maximum inhibitory activity of tyrosinase was 50%. The concentration (IC ₅₀ ), which can represent %, was calculated (Table 5). At this time, a reaction product to which ursolic acid was added was used as a positive control, and distilled water was used as a negative control.

[Equation 2]

Elastase activity rate (%) = 1-{(sample solution absorbance-negative control absorbance)/negative control absorbance×100(%)}

division Elastase activity inhibition ability (IC ₅₀ =mg/ml) positive control 56.50 Example 1 59.39 Example 2 57.97 Example 3 62.56 Example 4 64.91 Example 5 57.12 Comparative Example 1 67.93 Comparative Example 2 68.39 Comparative Example 3 64.78 Comparative Example 4 73.25 Comparative Example 5 69.22 Comparative Example 6 71.33 Comparative Example 7 55.98

Looking at the elastase inhibition activity measurement results in Table 7 above, it was confirmed that Examples 1 to 5 had an IC ₅₀ at an overall low concentration, although it was slightly lower than that of the positive control group.

On the other hand, Comparative Examples 1 to 5 had IC ₅₀ at relatively high concentrations compared to Examples 1 to 5, and compared to Examples, the elastase inhibitory effect was relatively low.

In addition, in the case of Comparative Examples 4 and 5, in which the reishi mushroom mycelium was used too little at a weight ratio of 1 or excessively at a weight ratio of 5 as a fermenting agent, the elastase inhibitory effect was relatively poor compared to other examples.

In addition, in the case of Comparative Example 6 (5 wt%), in which less than 10 wt% of the herbal medicine complex in the cosmetic was used, there was a problem of a significant lack of elastase inhibitory effect.

In the case of Comparative Example 7 (50% by weight), which is a cosmetic using more than 40% by weight of the complex fermented herbal medicine (50% by weight), the elastase inhibitory effect was somewhat reduced compared to Example 5.

Through this, it was confirmed that the cosmetic of the present invention can have the effect of improving skin wrinkles through the elastase inhibitory effect.

Experimental Example 4: Acne inhibition evaluation

5α-reductase is known to worsen acne symptoms by causing sebum overproduction.

The process of obtaining 5α-reductase used to confirm the inhibitory ability of the cosmetics prepared in Example 1 and Comparative Example 3 to inhibit 5α-reductase activity was performed at 0 to 2°C. The rats used in this test were the Sprague-Dawley strain. Mature females (8 weeks old) weighing 300 to 312 g were anesthetized with diethyl ether, killed by cervical dislocation, and then dissected to remove the liver. After washing with buffer solution (0.13M sucrose, 10mM Tris-HCl, 40ul/L 2-mercaptoethanol), it was placed in buffer solution and a suspension was prepared using a grinder. To separate 5α-reductase from the suspension, it was centrifuged (105,000 g, 1 hr, 4°C), then taken from the supernatant and stored at -70°C for use.

To evaluate the effect of inhibiting 5α-reductase activity, the method used by SUN ZU-YUE et al. (Indian Journal of Pharmacology, 1998) was used. A portion of the supernatant obtained in the same manner as above was taken and reacted with NADPH ₂ (coenzyme), testosterone (substrate), and extract (inhibitor) in the buffer solution, and the change in the amount of reduction of NADPH ₂ , which acts as a coenzyme, was measured using a UV spectrophotometer ( It was measured at 340 nm using a spectrophotometer. Using this data, Lineweaver-Burk Plot and Dixon Plot related to reaction rate, testosterone (substrate), and extract (inhibitor) were created, and Ki (inhibition constant) was calculated based on this. Ki is the concentration of the inhibitor that reduces the reaction rate Vmax and the half saturation constant Km to 1/2. The smaller the value, the better the inhibitory effect. Table 8 shows the Ki values of the cosmetics of Example 1 and the values of the control group.

division Ki(%W/V) azelaic acid 0.00890 Example 1 Cosmetic 0.00056 Comparative Example 3 Cosmetic 0.00752

Looking at the measurement results in Table 8, it was confirmed that the cosmetic of Example 1 had a better 5α-reductase inhibition effect than azelaic acid, which was a positive control. On the other hand, in the case of Comparative Example 3, 5α-reductase inhibition was superior to that of the positive control group, but compared to Example 1, it was confirmed that the inhibitory effect was significantly reduced.

Experimental Example 5: Evaluation of sebum production inhibition

Example 1 A lipogenesis experiment was performed to determine the extent to which cosmetics directly inhibit sebum production in vitro. The method of obtaining and primary culturing human sebaceous gland cells was the same as in Experimental Example 4, and for this experiment during subculture, 2×10 ⁵ cells were planted per well using a 24 well-plate. After culturing for 24 hours and allowing the cells to adhere to the wells, the cosmetics of Example 1 were dispersed in dimethylsulfoxide (DMSO) and treated at a final concentration of 0.05% (w/v) in the wells. After culturing for 72 hours, survival occurred. The number of cells was calculated, the amount of sebum produced was extracted and measured using the methanol:chloroform extraction method, and the total amount of sebum produced per 1 × 10 ⁴ cell was compared to compare the degree of sebum production inhibition, and the results were compared. It is shown in Table 9 below. The sebum extraction method used was the method of Zoboulis et al. (J. Invest. Dermatol., 1991; 24, 69-83), and included a methanol:chloroform extraction step, ultrasonic disruption step, filtering step, and nitrogen evaporation ( It includes a nitrogen evaporation step.

division Sebum production inhibition rate (%) azelaic acid 33.1% Example 1 Cosmetic 38.3%

As a result of the experiment, the cosmetic of Example 1 had a higher effect of inhibiting sebum production of human sebaceous gland cells than the positive control group, and it was confirmed that it is suitable for use as a functional cosmetic material that prevents or improves acne by suppressing sebum production. there was.

Experimental Example 6: Measurement of total polyphenols, total flavonoids, electron donating ability, and ABTS cation radical scavenging ability in cosmetics

Liphenol, flavonoid, electron donating ability, and ABTS cation radical scavenging ability of the cosmetic prepared in Example 1 were measured by requesting Suncheon Bio Healthcare Research Center Co., Ltd., and the results are shown in Tables 10 to 12 below. .

Total polyphenol content was measured by modifying AOAC's FolinDenis method (Folin & Denis, 1912), and total flavonoid content was measured using the aluminum colorimetric method (Anna & Krystyna 2014). Additionally, DPPH radical scavenging activity was measured using the Blois method, and ABTS cation radical scavenging activity was measured using ABTS cation radical decolorization assay.

Total polyphenol content (GAE ^a , μg/ml) 102.93±2.47 Total flavonoid content (QE ^b , μg/ml) 17.97±3.63 a=Expression of equivalent μg of gallic acid per ml of sample (n=3)
b=Expression of quercetin equivalent μg per ml of sample (n=3)

DPPH free radical scavenging ability (%) Vit.C
30μg/ml Example 1 Cosmetic 24-fold dilution 12-fold dilution 6-fold dilution 3-fold dilution Diluted 1.5 times 96.05±0.09 12.10±4.34 19.42±2.41 30.66±4.45 52.14±4.61 76.23±2.60

DPPH free radical scavenging ability was measured using a method of measuring antioxidant activity based on the degree of reduction that discolors from purple because the nitrogen atom of hydrazyl is unstable and has the property of easily accepting hydrogen atoms. Example 1 A removal ability of about 76% was confirmed in a solution diluted 1.5 times the cosmetic product. Although this is somewhat lower compared to the positive control Vit.C (L-ascorbic acid), it was confirmed that it has a high DPPH free radical scavenging ability.

ABTS cation radical scavenging ability (%) Vit.C
30μg/ml Example 1 Cosmetic 24-fold dilution 12-fold dilution 8-fold dilution 3-fold dilution Diluted 1.5 times 99.80±0.06 39.78±0.63 51.37±5.66 70.86±4.87 86.87±5.16 99.91±0.17

Looking at the ABTS cation radical scavenging ability measurement results in Table 12 above, it can be seen that the scavenging ability of about 99.9% was confirmed in the 1.5-fold diluted liquid of Example 1 cosmetic, which is equivalent to or equal to Vit.C (L-ascorbic acid), a positive control. It was confirmed that ABTS had a relatively higher cation radical scavenging ability.

Preparation Example 1: Preparation of cream-type cosmetics

A cosmetic was prepared by mixing 10% by weight of the fermented complex herbal medicine prepared in Preparation Example 2 and 90% by weight of the distilled extract from Preparation Example 1-1 in a homogenizer.

Using the above cosmetics, cream-type skin cosmetics having the ingredients and contents shown in Table 13 below were prepared.

division type Content (% by weight) Atopic skin improvement cosmetic 10% by weight of fermented complex herbal medicine and 90% by weight of distilled extract 73 skin conditioning agent glycerin 7.0 skin conditioning agent Caprylic/capric triride glyceride 6.5 antioxidant Sunflower seed oil, tocopherol 5.0 whitener Niacinamide 2.0 Moisturizing and anti-inflammatory pantherol 2.0 Skin soothing and skin barrier strengthening agent Centella asiatica extract 1.0 moisturizer Low molecular weight hyaluronic acid 1.0 Skin immunity enhancement and skin inflammation reliever Lactobacillus fermentation lysate 1.0 Wrinkle improvement agent peptide 1.0 Wrinkle improvement agent Adenosine 0.04

Preparation Example 2: Preparation of skin formulation cosmetics

A cosmetic was prepared by mixing 20% by weight of the fermented complex herbal medicine prepared in Preparation Example 2 and 80% by weight of the distilled extract from Preparation Example 1-1 in a homogenizer.

Using the above cosmetics, cosmetics for skin in a skin formulation having the ingredients and contents shown in Table 14 below were prepared.

division type Content (% by weight) Atopic skin improvement cosmetic 20% by weight of fermented complex herbal medicine and 80% by weight of distilled extract 66 moisturizer Dipropylene glycol 12 moisturizer Low molecular weight hyaluronic acid 3.0 Skin soothing and skin barrier strengthening agent Centella asiatica extract (powder) 5.0 Skin soothing and skin barrier strengthening agent Dandelion extract 3.0 Skin immunity enhancement and skin inflammation reliever Lactobacillus fermentation lysate 2.0 whitener Niacinamide 2.0 Wrinkle improvement agent peptide 1.0 Wrinkle improvement agent Adenosine 0.04 skin regeneration agent Madeca Society 0.04

Experimental Example 5: Skin stability experiment

The cream formulation cosmetics prepared in Preparation Example 1 and the skin formulation cosmetics prepared in Preparation Example 2 were commissioned by Intertek to evaluate their stability on human skin (whether inflammatory papules, pustules, blackheads, or whiteheads occurred).

A total of 22 people (average age 30 years, minimum age 24 years, maximum age 40 years) participated in the evaluation test, and changes such as skin troubles were evaluated after using cosmetics for 4 weeks, and the results are shown in Figure 1a. In addition, the skin stability evaluation results for test subjects 5, 14, and 20 for the first time and after 4 weeks of use of cosmetics are shown in Figures 1A to 1D. In FIGS. 1A to 1D, W0 means the first day, and W4 means 4 weeks after using the cosmetics.

1A and 2B, the number of whiteheads (millet acne), blackheads, purulent acne, and pustules (acne) was significantly reduced (P<0.001), proving that this product is effective in the non-comedogenic test. After 4 weeks of using the cosmetics of Preparation Examples 1 and 2, the sum of the four lesions did not significantly increase compared to the baseline, confirming the results that the cosmetics of Preparation Examples 1 and 2 had a safety effect.

Experimental Example 6: Analysis of residual pesticides in cosmetics

The cream formulation cosmetics prepared in Preparation Example 1 and the skin formulation cosmetics prepared in Preparation Example 2 were commissioned by Intertek to analyze the content of insecticidal substances (320 types) that are harmful to the skin and human body, and the results are shown in Figures 2a to 2. 2b (cream formulation) and Figures 3a to 3b (skin formulation).

As a result of the analysis, it was found that the cosmetics of Preparation Example 1 and Preparation Example 2 did not contain 320 types of insecticidal substances.

Claims (10)

Distilled extract of fermented complex herbal medicine; and complex herbal medicine fermentation products;
The complex herbal medicine includes Dendropanax Morbifera, Eriobotrya Japonica Leaf, Adenophora Stricta Leaf & Root, Hordeum Vulgare, Saururus Chinensis, and Artemisia seeds ( Contains Artemisia Annua seed and Tea Tree Leaf,
The Hwangchil tree is an acne improving cosmetic comprising a mixture of leaves, stems and fruits of the Hwangchil tree. The cosmetic for improving acne according to claim 1, comprising 10 to 40% by weight of the fermented complex herbal medicine and the remaining amount of distilled extract of the fermented complex herbal medicine. The method of claim 1, wherein the composite herbal medicine includes 10 to 20% by weight of loquat leaves, 10 to 20% by weight of Dangjangi leaves and roots, 10 to 20% by weight of barley sprouts, 5 to 15% by weight of Trifolium primrose, and 5 to 5% by weight of Artemisia seeds. An acne-improving cosmetic comprising 15% by weight, 3 to 7% by weight of tea tree leaves, and the remaining amount of Hwangchil tree. The acne improving cosmetic according to claim 1, wherein the distilled extract is a distilled extract obtained by vacuum distillation of a fermented product obtained by fermenting a hot water extract of a complex herbal medicine. The method of claim 1, wherein the fermented composite herbal medicine is a fermented product obtained by fermenting a low-temperature hot water extract of the composite herbal medicine,
The low-temperature hot water extract is an acne-improving cosmetic product obtained by mixing the complex herbal medicine and water at a weight ratio of 1:3.0 to 6.0 and then low-temperature heat treatment at 40 to 60° C. for 16 to 24 hours. A cosmetic for skin or scalp comprising the cosmetic of any one of claims 1 to 5. Step 1 of preparing a dried composite herbal medicine by ultrasonic washing a mixture of Hwangchil tree, loquat tree leaves, Tangjangi leaves and roots, barley sprouts, Trichytense aerobic herbacea, Artemisia seeds and tea tree leaves and then drying them;
Step 2 of producing a distilled extract and a fermented complex herbal medicine, respectively, using the complex herbal medicine;
Performing a process including 3 steps of mixing the distilled extract of the complex herbal medicine fermentation product and the complex herbal medicine fermentation product to prepare a mixed solution,
A method of producing an acne-improving cosmetic, characterized in that the Hwangchil tree in step 1 includes a mixture of leaves, stems, and fruits of the Hwangchil tree. The method of claim 7, wherein the distilled extract of the fermented complex herbal medicine is,
Step 2-1 of steaming the complex herbal medicine by applying steam at 80 to 90°C for 2 to 4 hours;
Step 2-2 of mixing steamed composite herbal medicine and water at a weight ratio of 1:2.5 to 5.0, followed by heat treatment at 85 to 95°C for 10 to 14 hours;
Steps 2-3 of filtering the heat-treated product and then cooling it to obtain a hot water extract;
Steps 2-4 of mixing the hot water extract with a fermentation agent containing makgeolli yeast and reishi mushroom mycelium or mycelium of Sanghwang mushroom, fermenting at 25 to 40°C for 48 to 60 hours, and then filtering to obtain a fermented product; and
A method for producing an acne-improving cosmetic, characterized in that it is manufactured by performing a process including steps 2-5 of performing a vacuum distillation extraction process on the fermented product to obtain a distilled extract, and then filtering the product to obtain a filtrate. The method of claim 7, wherein the fermented complex herbal medicine is,
Step 2-1 of mixing the composite herbal medicine and water at a weight ratio of 1:3 to 5, followed by low-temperature heat treatment at 40 to 60°C for 16 to 24 hours;
Step 2-2 of filtering the low-temperature heat-treated product and then cooling it to obtain a low-temperature hot water extract;
Steps 2-3 of mixing low-temperature hot water extract with a fermentation agent containing makgeolli yeast and reishi mushroom mycelium or mycelium of Sanghwang mushroom, fermenting at 25 to 40°C for 45 to 60 hours, and then filtering to obtain a fermented product; and
A method for manufacturing an acne-improving cosmetic, characterized in that it is manufactured by performing a process including steps 2-4 of heat-treating the obtained fermented product to kill the fermented product and then obtaining a filtered filtrate. The method of claim 8 or 9, wherein the makgeolli yeast includes yeast enzyme bacteria,
A method for producing an acne-improving cosmetic, characterized in that 2.0 to 5.0 parts by weight of the fermentation agent is mixed with 100 parts by weight of the hot water extract.