KR20150023601A - Composition Containing Extracts of Saururus chinensis - Google Patents

Abstract

Disclosed is a composition which contains an extract of Saururus chinensis as an active ingredient. The composition has an effect of controlling pigmentation and scar of acne, and is widely used in cosmetic, food or medical fields. The composition for skin external use is characterized by having 0.5-20 wt% of the extract of Saururus chinenesis for controlling inflammation of acne and controlling serum production of acne based on total weight of the composition.

Description

Composition Containing Extracts of Saururus chinensis < RTI ID = 0.0 >

The present invention relates to a composition containing an extract of Saururus chinensis as an active ingredient.

In general, acne is caused by sebum excess associated with male hormone secretion. Specifically, sebum is generated by the male hormone and hyperkeratinization of the pores is induced, so that the entrance of the pore narrows or becomes obstructed in severe cases. When the pore opening becomes narrow or clogged, the sebaceous pores in the pores are prevented from being discharged to the outside, and microfeatures are formed. Propionibacterium acnes, an anaerobic bacteria living in the pores, have.

The inflammatory reaction causes erythema, tingling, or swelling, and in severe cases hyperpigmentation may occur. In addition, when the acne area is squeezed or pressed, the risk of acne scarring is increased as the cotton bum pops and the inflammation worsens or tissue damage occurs.

Such acne inflammation can be caused by various causes, for example, various additives contained in cosmetics may be left on the skin and cause acne inflammation. Skin inflammation may also be caused by sebum, sweat or ultraviolet rays emitted from the body.

In acne inflammation caused by various causes, research and development are concentrated on the treatment of inflammation itself. However, even if the inflammation is treated, the skin erythema or scar resulting therefrom remains, resulting in poor cosmetic results. Therefore, in the present invention, studies were conducted to inhibit or prevent pigmentation and scarring caused by acne inflammation.

An object of one embodiment of the present invention is to provide a composition for inhibiting acne pigmentation.

Another object of one embodiment of the present invention is to provide a composition for suppressing acne scars.

Another object of the present invention is to provide a composition for aftercare for acne sequelae.

The composition according to one embodiment of the present invention is characterized by containing Saururus chinensis extract as an active ingredient.

The composition according to one embodiment of the present invention has an effect of inhibiting acne pigmentation and / or scarring. As a result, it can be used in various fields such as cosmetics, food or medicine.

(GM-CSF), Figure 2 (IL-1 alpha), Figure 3 (IL-8), and Figure 4 (TNF- [alpha]) show that inflammatory cytokines of Saururus chinensis extract according to one embodiment of the present invention Inhibitory effect;
5 is a graph showing inhibitory effect on sebum production of Saururus chinensis extract according to an embodiment of the present invention;
FIG. 6 is a graph showing an effect of inhibiting acne pigmentation of Saururus chinensis extract according to an embodiment of the present invention;
FIGS. 7 to 9 are graphs showing an effect of inhibiting acne scars of Saururus chinensis extract according to an embodiment of the present invention.

The composition according to the present invention contains Saururus chinensis extract as an active ingredient. Compositions containing Saururus chinensis extract are effective in preventing or inhibiting acne pigmentation and / or acne scarring. In one embodiment, the composition according to the present invention is an acne pigmentation-inhibiting composition containing Saururus chinense extract as an active ingredient. In another embodiment, the composition according to the present invention is an acne scar inhibiting composition containing Saururus chinensis extract as an active ingredient.

Saururus chinensis ) is a kind of perennial plant of the dicotyledonous plant Peppercorn Saururus chinensis. The rootstock is white and extends sideways in the mud. The height of the stem is 50-100 cm, and white flowers bloom in June-August. It grows mainly in wetlands and is called Saururus chinensis because its roots, leaves and flowers are white. It is known to be effective against jaundice, hepatitis, etc., and is distributed in Korea, Japan, and China.

The Saururus chinensis extract can be extracted by various conventional methods without any limitation. In one embodiment, the Saururus chinensis extract may be extracted with an organic solvent such as distilled water, C ₁ to C ₅ anhydrous or hydrous lower alcohols. For example, it is possible to rinse the Saururus chinensis root and then extract it at room temperature using ethanol after drying. The extract may further be subjected to a purification process including fractionation, filtration and precipitation.

In one embodiment, the Saururus chinensis extract is effective in inhibiting gelatinase. Gelatinase is a type of protein hydrolyzing enzyme expressed in various kinds of fungi and yeast. It has a function of liquefaction of gelatin. There are no particular restrictions on the type of gelatinase, and examples of gelatinase genes expressed in humans include matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-2 (MMP-2). In yet another embodiment, the Saururus chinensis extract is effective in inhibiting MMP-9 and / or MMP-2. The composition according to the present invention prevents or suppresses acne scars by inhibiting gelatinase, specifically MMP-9 and / or MMP-2.

The composition according to the present invention can be utilized in various fields without particular limitation, for example, it can be formulated as an external preparation for skin, specifically, in the form of a cosmetic composition or a pharmaceutical composition.

The composition for external application for skin according to the present invention may be in the form of a solution, suspension or emulsion in an oil or an aqueous medium, or may be formulated in the form of a dry powder which is dissolved in sterile, exothermic water before use. The water-in-oil type emulsion is prepared by emulsifying a mineral oil such as vegetable oil or liquid paraffin in an oil phase such as olive oil, natural acid phospholipids such as soybean lecithin and those derived from anhydride or fatty acid esters such as sorbitan monooleate, Such as ethylene sorbitol monooleate, are condensed with ethylene oxide as emulsifiers to emulsify the active ingredient.

In one embodiment, in the composition for external application for skin according to the present invention, the content of Saururus chinensis extract may be 0.5 to 20% by weight, based on the total weight of the composition. When the content of the extract is less than 0.5% by weight, the effect of the control and improvement of the skin condition is insignificant. When the content of the extract is more than 20% by weight, the efficiency due to the addition of the extract is decreased and the formulation stability and other problems may be caused.

When the composition is formulated in the form of a cosmetic, it can be used for the purpose of preventing or improving acne pigmentation. It can also be used for the purpose of preventing or improving the occurrence of acne scars. The cosmetic formulation may be, for example, a cosmetic composition having a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic composition, , Creams, patches, or spray formulations. Further, in the respective formulations, other ingredients may be selected and mixed without difficulty by those skilled in the art depending on the kind of the other cosmetic composition or the purpose of use.

The present invention also provides a pharmaceutical composition comprising said composition. The pharmaceutical composition comprising the composition according to the present invention may be a composition for improving or inhibiting acne pigmentation and / or acne scars.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, soft capsules, powders, granules, powders, emulsions, syrups and pellets. . Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

In addition, the dosage of the active ingredient will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art. Typical dosages are from 0.001 mg / kg / day to 2000 mg / kg / day, more specifically from 0.5 mg / kg / day to 1500 mg / kg / day.

In one embodiment, the present invention provides a composition for aftercare containing Saururus chinensis extract. More specifically, the present invention provides an aftercare composition for managing skin condition and / or aftereffects such as scarring caused by skin troubles caused by acne in a comprehensive manner and improving the skin condition.

In one embodiment, in an aftercare composition according to the present invention, the content of Saururus chinensis extract may be 0.5 to 20% by weight, based on the total weight of the composition. When the content of the extract is less than 0.5% by weight, the effect of the control and improvement of the skin condition is insignificant. When the content of the extract is more than 20% by weight, the efficiency due to the addition of the extract is decreased and the formulation stability and other problems may be caused.

In the present invention, 'after care' means comprehensive after-care for managing and improving the skin condition. For example, if you enjoy swimming in beaches, rivers or valleys during the holiday season, or for a long time, such as tanning or tanning, your skin may become overexposed to ultraviolet light and cause inflammation. In addition, various additives such as sunscreen or oil applied to the skin may cause acne inflammation by remaining on the skin. In addition, skin troubles such as acne inflammation can be caused by various causes in daily life. After care is meant to collectively refer to the skin care and skin care management for improving the skin condition, including acne inflammation. In addition, the composition for aftercare is meant to encompass a composition for sticking, depositing or applying to the skin for aftercare.

In one embodiment, the present invention provides a food additive, a functional food, a health food or the like containing the composition. Specifically, the composition may be a composition for improving or inhibiting acne pigmentation and / or scarring.

The composition according to the present invention provides various types of food additives or functional foods containing them. It can be processed into fermented milk, cheese, yogurt, juice, probiotic agent and health supplement food including the above composition, and it can be used in various other food additives.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

[ Example  1] Preparation of Saururus chinensis extract

Saururus chinensis root was washed well and then extracted with 10 times ethanol at room temperature for 7 days. The extract was filtered through a filter of 250 mesh (3 mu m) and then concentrated at 60 < 0 > C. The concentrate was dissolved in water, and ethyl acetate layer was separated by adding ethylacetate and then concentrated at a temperature of 60 ° C. To the concentrate was added methylene chloride. Then, the mixture was precipitated twice in a mixed solution of ethyl acetate and hexane to remove impurities, and then the fractions were separated into columns. The column fractions were repeatedly subjected to concentration at 60 ° C and concentration under reduced pressure at 30 ° C to completely remove the residual solvent, and then the solution was dissolved in butylene glycol to make a 2% solution. The resulting solution was subjected to additional filtration and precipitation to prepare Saururus chinensis extract.

[ Experimental Example  1] cells ( Cell ) Culture conditions

Hamster sebocytes were extracted from the sebaceous glands of five-week-old male golden hamster ears. For specific extraction methods, see Sato et al. (J Invest Dermatol 2001, 117: 965-70). The extracted sebaceous cells were cultured in a culture flask so as to be 2.35 x 10 ⁴ / cm ² .

The culture medium was prepared by adding fetal bovine serum (JRH Bioscience, Tokyo, Japan) to DMEM / Ham's F12 medium (DMEM / Ham's F12 medium; (DMEM / F12) (Invitrogen, Carlsbad, CA) (Invitrogen) and 10 nM of rhEGF (recombinant human epidermal growth factor; Progen Biotechnik GmbH, Heidelberg, Germany).

Penicillin 100 mg / ml and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were used as antibiotics. Hamster sebaceous cells were attached to the culture flask for 24 hours and cultured at 37 ° C and 5% CO ₂ .

HaCaT, a human keratinocyte cell line, was prepared by adding 10% fetal bovine serum (JRH Bioscience, Tokyo, Japan) to DMEM medium (Invitrogen, Carlsbad, Calif.), Adding penicillin 100 mg / The cells were cultured in a medium supplemented with 100 mg / ml streptomycin (all from GIBCO, Milan, Italy) at 37 ° C and 5% CO 2.

Melan-a melanocyte melanocyte derived from C57BL / 6J mouse was cultured in RPMI-1640 (Invitrogen, Carlsbad, CA) with fetal bovine serum (JRH Bioscience, Tokyo, 10% CO ₂ (100 μl) was added to the medium supplemented with 200 nM of TPA (tetradecanoyl phorbol acetate), 100 mg / ml of penicillin and 100 mg / ml of streptomycin (all from GIBCO, Milan, Italy) Lt; / RTI >

[ Experimental Example  2] Acne castle  Inflammation inhibitory effect in vitro  evaluation

In order to evaluate effectively the inhibitory effect of Saururus chinensis extract on acne inflammation, in vitro inflammation model of environment similar to human acne lesion was used by using sebaceous gland cells and keratinocytes together. HaCaT keratinocytes and golden hamster sebaceous cells were inoculated in a 24 well culture plate at 3.75x10 ⁴ and 6.0x10 ⁴ , respectively. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. At this time, the medium was incubated with fetal bovine serum (JRH Bioscience, Tokyo, Japan) supplemented with DMEM / Ham's F12 medium (DMEM / Ham's F12 medium; (DMEM / F12) (Invitrogen, Carlsbad, CA) (Penicillin 100 mg / ml) and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were added to each well.

Day of linoleic acid with irritants in the last cell for causing acne inflammation (linoleic acid) 50 μM, arachidonic acid (arachidonic acid) 50 μM, dihydrotestosterone (dihydrotestosterone) 10 nM, acne bacteria (P. acnes) treated with 0.5% Respectively. At the same time, Saururus chinensis extract was treated to observe the acne inflammation inhibitory effect by stimulus source. DMSO was used as a negative control and 100 nM isotretinoin, a widely used drug for acne treatment, was used as a positive control. (GM-CSF) (granulocyte macrophage colony-stimulating factor), IL-1α (interleukin-1alpha), and IL-1α, which are the inflammatory cytokines dissolved in the culture medium Concentrations of IL-8 (interleukin-8) and TNF-α (tumor necrosis factor-alpha) were measured in a 50 μl medium using a multiplex bead-based cytokine assay.

More specifically, the following procedure was carried out: The solution in the 96-well filter plate, which had previously been soaked with the wash buffer, was all sucked off using a vacuum pump. Next, the cell culture was reacted with an antibody-conjugated bead for 30 minutes. When the reaction was completed, a detection antibody was added and the reaction was further continued for 30 minutes. Then streptavidin-phycoerythrin (Streptavidin-PE) was added to each well and waited for 30 minutes. The unbound Streptavidin-PE was removed using wash buffer and the amount of beads attached to the cytokine was analyzed using a Bio-plex 200 device.

The results of the analysis are shown in FIG. 1 (GM-CSF), FIG. 2 (IL-1α), FIG. 3 (IL-8), and FIG. 4 (TNF-α). Referring to FIGS. 1 to 4, it can be seen that the Saururus chinensis extract according to the present invention inhibits inflammatory cytokines in a concentration-dependent manner. However, in FIG. 2 (IL-1α), the effect was more excellent at a concentration of less than 50 ppm.

[ Experimental Example  3] Acne castle  Sebum production inhibitory effect in vitro  evaluation

In order to evaluate effectively the inhibitory effect of Saururus chinensis extract on the production of acne vulgaris, an in vitro model of an environment similar to human acne lesion was used using both sebaceous and keratinocytes. HaCaT keratinocytes and golden hamster sebaceous cells were inoculated in a 24 well culture plate at 3.75x10 ⁴ and 6.0x10 ⁴ , respectively. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. At this time, the medium was incubated with fetal bovine serum (JRH Bioscience, Tokyo, Japan) supplemented with DMEM / Ham's F12 medium (DMEM / Ham's F12 medium; (DMEM / F12) (Invitrogen, Carlsbad, CA) (Penicillin 100 mg / ml) and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were added to each well.

Day of linoleic acid with irritants in the last cell for causing acne inflammation (linoleic acid) 50 μM, arachidonic acid (arachidonic acid) 50 μM, dihydrotestosterone (dihydrotestosterone) 10 nM, acne bacteria (P. acnes) treated with 0.5% Respectively. At the same time, Saururus chinensis extract was treated to observe the acne inflammation inhibitory effect by stimulus source. DMSO was used as a negative control and 100 nM isotretinoin, a widely used drug for acne treatment, was used as a positive control. The amount of triglyceride produced in the cells was measured using Oil Red O staining method after treatment with stimulus source and extracts.

More specifically, the following procedure was carried out: Cells washed with PBS were fixed with 3.7% formaldehyde solution for 30 minutes. The fixed cells were washed 3 times with PBS, once with 70% ethanol, and stained with 0.4% Oil Red O solution for 30 minutes. The stained cells were washed once with 70% ethanol and three times with PBS. Then, isopropanol dyed Oil Red O solution was re-dissolved and absorbance was measured at 520 nm using a spectrophotometer.

The results of quantification are shown in Fig. Referring to FIG. 5, the Saururus chinensis extract inhibited sebum in a concentration-dependent manner, and showed an effective sebum inhibitory effect at a concentration of 50 ppm or more.

[ Experimental Example  4] Acne castle  Pigment inhibition effect in vitro  evaluation

In order to efficiently evaluate the inhibitory effect of Saururus chinensis extract against acne pigmentation, an in vitro model of environment similar to pigmentation induced by human acne inflammation was used, using melanin - forming cells. Melan-a melanin-forming cells were inoculated in a 24-well culture plate at 5.0 x 10 ⁴ cells / well. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. At this time, 10% of fetal bovine serum (JRH Bioscience, Tokyo, Japan) was added to RPMI-1640 (Invitrogen, Carlsbad, Calif.) And 200 nM of tetradecanoyl phorbol acetate (TPA), 100 mg of penicillin / ml, streptomycin 100 mg / ml (all from GIBCO, Milan, Italy).

One day later, 50 μM of linoleic acid, 50 μM of arachidonic acid, 10 nM of dihydrotestosterone, and 0.5% of acne ( P. acnes ) were treated as stimulants to cause acne pigmentation And cultured for 5 days. After that, the extract of Saururus chinensis was treated to observe the effect of stimulants on the inhibition of acne pigmentation. DMSO was used as a negative control, and hydroquinone (1 μM) and kojic acid (200 ppm) were used as positive control groups, respectively. Hydroquinone is a specialty medicine used as a melamine inhibitor. In addition, kojic acid is recognized as a whitening effect and may be used as one of raw materials for a cosmetic composition. However, Kojisan may cause a strong irritation to the skin, and recently it has been reported that it may cause various cancers. After 5 days of treatment, the extract was treated with 1 N NaOH to dissolve the melanin, and the absorbance was measured at 405 nm using a spectrophotometer.

The measurement results are shown in Fig. Referring to FIG. 6, the Saururus chinensis extract according to the present invention has an effect of inhibiting pigment deposition in a concentration-dependent manner. Especially, at 5 ppm concentration, the inhibition effect of pigmentation was very good and the degree of pigmentation was lower than that of control group without stimulus source. As a result, it was confirmed that the Saururus chinensis extract according to the present invention has an excellent inhibitory effect on pigment deposition and a whitening effect.

[ Experimental Example  5] Acne castle  Scar inhibitory effect in vitro  evaluation

In order to evaluate effectively the acne scar inhibitory effect of Saururus chinensis extract, we used an in vitro acne scar model of environment similar to human acne lesion using sebaceous gland cells. 6.0x10 < ⁴ > of golden hamster ovary cells were inoculated on a 24 well culture plate. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. The culture medium was supplemented with fetal bovine serum (JRH Bioscience, Tokyo, Japan) supplemented with DMEM / Ham's F12 medium (DMEM / Ham's F12 medium (1: 1) (DMEM / F12) (Invitrogen, Carlsbad, CA) (Penicillin 100 mg / ml) and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were added to each well.

One day old cells were treated with 50 μM of linoleic acid, 50 μM arachidonic acid, 10 nM of dihydrotestosterone, and 0.5% of acne ( P. acnes ) as stimulants to cause acne inflammation . At the same time, Saururus chinensis extract was treated to observe the acne scar inhibitory effect by stimulants. DMSO was used as a negative control, and 100 nM of isotretinoin, a widely used drug for treating acne, was used as a positive control. Gelatin degradation activity of matrix metalloproteinase (MMP) dissolved in culture broth was measured by gelatin zymography method one day after treatment with stimulus source and extract.

More specifically, the following procedure was carried out: 5 μl of the cell culture and 5 μl of a Tris-Glycine sample buffer (Invitrogen, Carlsbad, Calif.) Were mixed to obtain a 10% The acrylamide gel was electrophoresed at 150 V for 2 hours. The gel was separated from the frame and allowed to react in a renaturing buffer for 30 minutes and a developing buffer for 30 minutes at room temperature. The gel was then added to a developing buffer and incubated at 37 ° C for 8 hours Lt; / RTI > The developing buffer was removed, washed three times with PBS, and stained with SimplyBlue (Invitrogen, Carlsbad, CA) for 1 hour. The stained gel was washed and observed with distilled water until the band and background were sufficiently distinguished.

The experimental results are shown in Figs. 7, MMP-9 and MMP-2 isolated by electrophoresis can be confirmed. In FIGS. 8 and 9, it can be seen that the Saururus chinensis extract according to the present invention has MMP-9 and MMP-2 inhibitory effects. This inhibitory effect is comparable to the treatment of isotretinoin, which is widely used as an acne medication.

Hereinafter, formulation examples of the composition will be described, but the present invention is not intended to be limited thereto but is specifically described.

[Formulation Example 1] Preparation of soft capsules

A soft capsule filling liquid was prepared by mixing 80 mg of Saururus chinensis extract, 9 mg of vitamin E, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of vegetable hardening oil, 4 mg of yellow bean and 9 mg of lecithin and mixing them according to a conventional method. 400 mg per capsule was filled to prepare a soft capsule. Separately from the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin and 10 parts by weight of sorbitol solution, and filled with the filling solution to prepare a soft capsule containing 400 mg of the composition of the present invention .

[Formulation Example 2] Preparation of tablet

After mixing with 80 mg of Saururus chinense extract, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose and granulating with a fluid bed drier, 6 mg of sugar ester was added Respectively. 504 mg of these compositions were tabletted by a conventional method to prepare tablets.

[Formulation Example 3] Preparation of Drinking Agent

After mixing 80 mg of Saururus chinense extract, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid and 25 g of liquid oligosaccharide, 300 ml of purified water was added to each bottle to make 200 ml each. And then sterilized at 130 DEG C for 4 to 5 seconds to prepare a beverage.

[Formulation Example 4] Preparation of granules

The extracts were prepared by mixing 80 mg of Saururus chinensis extract, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose and 550 mg of starch, forming into granules using a fluidized bed granulator and filling the pellets.

[Formulation Example 5] Production of softening longevity (skin lotion)

A mixture of 255 mg of purified water, 0.3 mg of Saururus chinensis extract, 6.0 mg of butylene glycol, 6.0 mg of propylene glycol, 0.3 mg of carboxyvinyl polymer, 0.6 mg of phage-12 nonylphenyl ether, 1.2 mg of polysorbate 80, 30.0 mg of ethanol, mg, and antioxidants, coloring matters, and flavorings, the above components were added in the prescribed amounts according to a conventional method for preparing the softening agent, and uniformly mixed to prepare the desired softening agent.

[Formulation Example 6] Production of nutritional lotion (milk lotion)

(234 mg of purified water, 0.3 mg of Saururus chinensis extract, 12.0 mg of beeswax, 4.5 mg of polysorbate 60, 4.5 mg of sorbitan sesquioleate, 1.5 mg of liquid paraffin, 15 mg of Montana 202 (manufacturer: Seppic), 9.0 mg of glycerin, , 9.0 mg of propylene glycol, 0.3 mg of carboxyvinyl polymer, 0.3 mg of triethanolamine and 0.6 mg of each of antiseptic, colorant and fragrance were added in the prescribed amounts according to a conventional method of producing a nutritional lotion, After mixing, the desired nutritional lotion was prepared.

[Formulation Example 7] Production of massage cream

Purified water 70 mg, Saururus chinensis extract 0.3 mg, wax 30.0 mg, polysorbate 60 4.5 mg, phage 60 hardened castor oil 6.0 mg, sorbitan sesquioleate 2.4 mg, liquid paraffin 120.0 mg, squalane 15.0 mg, Montana 202 : Seppic), 15.0 mg of glycerin, 12.0 mg of butylene glycol, 12.0 mg of propylene glycol, 0.6 mg of triethanolamine and a small amount of each of preservatives, coloring matters and perfumes according to a conventional method for producing a massage cream. The ingredients were added in the proportions presented and mixed uniformly, and the desired massage cream was prepared.

[Formulation Example 8] Preparation of pack

A solution of 0.5 mg of Saururus chinense extract, 1.0 mg of beta-1,3-glucan, 13.0 mg of polyvinyl alcohol, 0.2 mg of sodium carboxymethylcellulose, 5.0 mg of glycerin, 0.1 mg of allantoin, 6.0 mg of ethanol, 0.3 mg of fingi-12nonylphenyl ether, 0.3 mg of Solvate 60, and purified water were added to each small amount of the preservative, coloring matter and perfume, and the above components were added in the prescribed amounts according to a conventional method for producing a massage cream, uniformly mixed, .

[Formulation Example 9] Preparation of gel

0.1 mg of beta-1,3-glucan, 0.05 mg of sodium ethylenediaminetetraacetate, 5.0 mg of glycerin, 0.3 mg of carboxyvinyl polymer, 5.0 mg of ethanol, 0.5 mg of fat-60 hardened castor oil, 0.3 mg of triethanolamine , Purified water was added to each small amount of preservatives, coloring matters and flavorings, and the above components were added in the prescribed amounts according to a conventional method for preparing a pack, and uniformly mixed to prepare a desired pack.

[Formulation Example 10] Ointment in external preparation for skin

2.0 mg of Saururus chinensis extract, 10.0 mg of beta-1,3-glucan, 10.0 mg of beeswax, 5.0 mg of polysorbate 60, 2.0 mg of hydrogenated castor oil, 2.0 mg of sorbitan sesquioleate, 5.0 mg of petroleum jelly, 5.0 mg of liquid paraffin Purified water was added to each small amount of each of small amounts of mg, squalane 5.0 mg, shea butter 3.0 mg, caprylic / capric triglyceride 5.0 mg, glycerin 10 mg, propylene glycol 10.2 mg, triethanolamine 0.2 mg and preservative, dye, , And the above components were added in the prescribed proportions according to the conventional ointment preparation method and uniformly mixed to prepare the desired ointment agent.

 [Formulation Example 11] Preparation of drug (patch) for topical administration

1.0 mg of Saururus chinensis extract, 3.0 mg of beta-1,3-glucan, 20.0 mg of hexylene glycol, 0.7 mg of diethylamine, 1.0 mg of polyacrylic acid (Carbopol 934P), 0.1 mg of sodium sulfite, polyoxyethylene lauryl ether (EO = 9), 1.0 mg of polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000), 2.5 mg of viscous paraffin oil, 2.5 mg of caprylic acid ester / caproic acid ester (Cetiol LC) and 3.0 mg of polyethylene glycol 400 , Purified water was added, and the above ingredients were added in the prescribed amounts in accordance with the conventional method for producing a patch, and then mixed uniformly to prepare a desired patch.

Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

Claims (6)

A composition for suppressing acne inflammation containing an extract of Saururus chinensis as an active ingredient. A composition for inhibiting the production of an acne-causing sebum containing an extract of Saururus chinensis as an active ingredient. A composition for external application for skin comprising the composition according to claim 1 or 2. The method of claim 3,
Wherein the content of Saururus chinensis extract is 0.5 to 20% by weight based on the total weight of the composition. The method of claim 3,
Wherein the composition is a cosmetic formulation. A food additive or health food composition comprising the composition according to claims 1 or 2.

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