KR101440675B1 - Composition for Improving Skin Wrinkle Comprising Ginseng Oil as Active Ingredient - Google Patents

Abstract

The present invention relates to a composition for improving skin wrinkles containing red ginseng oil as an active ingredient. Red ginseng oil, which is a lipid soluble component isolated from red ginseng or red ginseng root, inhibits the expression of MMP-2 and MMP-9 induced by UV exposure. It can contribute to improvement of wrinkles of the skin. Therefore, the red ginseng oil of the present invention can be usefully used as an active ingredient of a composition for improving skin wrinkles.

Description

TECHNICAL FIELD The present invention relates to a composition for improving skin wrinkles containing red ginseng oil as an active ingredient,

The present invention relates to a composition for improving skin wrinkles containing red ginseng oil as an active ingredient.

Skin protects the organs in the body from changes in temperature and humidity and from external stimuli such as ultraviolet rays (UV). However, the constant physical and chemical stimuli received from the outside deteriorate the skin function, promote the aging of the skin, and the aging of the skin accompanies the deterioration of the skin constituents as a whole. The most common cause of wrinkles is the degradation of collagen that forms the matrix in the dermis of the skin. Collagen in the skin may be degraded as aging progresses. However, collagen, gelatin and elastin are easily degraded by the activity of Matrix Metallo Proteinase (MMP) due to external stimuli such as UV, Is reported to increase. Therefore, in recent years, many products have been developed that inhibit the inhibition of the production of MMP and the activity thereof, thereby suppressing the decomposition of collagen fundamentally generated.

On the other hand, MMP is a calcium and zinc-dependent endopeptidase secreted from cells such as polymorphonuclear neutrophils, macrophages, fibroblasts, etc., and acts at neutral pH, Which uses various cell substrates. Mainly in skin, enzymes such as MMP-1 (Collagenase), MMP-2 (Gelatinase A) and MMP-9 (Gelatinase B) act. Therefore, the method of inhibiting wrinkle formation can prevent skin aging and wrinkles by promoting collagen and gelatin synthesis, or inhibiting the production and activity of their degrading enzymes.

The active ingredients for skin wrinkle improvement or anti-aging currently being developed can not be used as a cosmetic raw material or are very unstable, and it is not easy to deliver to the skin, so a special stabilization system and delivery system are required. And is not visible. Recently, attention has been increasingly focused on a skin protecting agent containing a retinoid. Retinoids have been used as means for solving photoaging phenomena such as wrinkles, skin thickening, sagging, and elasticity reduction, which are accumulated as daylight. However, retinoids are very unstable compounds, and they are sensitive to UV, moisture, heat, and oxygen. Therefore, it is necessary to study the development of effective ingredients derived from natural materials to solve these problems.

On the other hand, it is known that saponin and non-saponin components are present in red ginseng. The saponin component has been found to have anti-aging and anti-aging properties such as wrinkle and elasticity improvement. However, The research on efficacy is still small. Therefore, in the red ginseng containing physiologically active substances, there is a desperate need to develop a natural raw material of a cosmetic for improving wrinkles, more effective, easy and safe among the non-saponin ingredients that have not been reported on the effect of improving skin wrinkles -2009-0080234; 10-2011-0108735).

It is an object of the present invention to provide a composition for improving skin wrinkles containing red ginseng oil as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin wrinkles containing red ginseng oil as an active ingredient.

As used herein, the term "skin" refers to a tissue covering the body surface of a human body, and is used to encompass the broadest concept including not only the face or body but also scalp and hair.

In a preferred embodiment of the present invention, the red ginseng is preferably pale yellow-brown or light reddish brown ginseng which is steamed and dried without extracting the skin of the fresh ginseng, preferably 6-year old ginseng. Red ginseng is produced by a special processing technology, and it can be used in various processes such as anti-cancer component (ginsenoside Rh2), cancer cell toxin activity inhibition component (panaxatriol), platelet aggregation inhibiting component (maltol) Physiologically active ingredients are produced and contain trace amounts of essential oils.

The red ginseng oil of the present invention can be extracted from red ginseng powder or red ginseng powder. The red ginseng may be any one produced through a conventional process. The red ginseng powder may be obtained by extracting the extract from red ginseng, washing the obtained red ginseng with water, and then drying it with hot air to obtain a powder.

The red ginseng oil may be extracted by a conventional method such as room temperature extraction, warming extraction, ultrasonic extraction, supercritical extraction, or the like, and a nonpolar solvent may be used to effectively extract lipid soluble components from red ginseng. As the nonpolar solvent, hexane, chloroform, dichloromethane, ethyl acetate, and a mixed solvent thereof may be used, but hexane is preferably used, but not limited thereto.

According to a preferred embodiment of the present invention, the red ginseng oil of the present invention is prepared by adding 60 ml to 1 L of a non-polar solvent to about 1 kg of red ginseng or red ginseng powder and heating at 4 to 70 ° C, preferably 24 to 65 ° C, Min to 24 hours, preferably 30 minutes to 2 hours. The obtained extract is cooled, filtered, and further concentrated under reduced pressure to remove the solvent.

The thus-prepared red ginseng oil of the present invention contains various components and contains as essential components fat, vegetable sterol, phospholipid and polyacetylene, preferably 70.0 to 90.0 parts by weight of fat, 0.1 to 15.0 parts by weight of vegetable sterol, 10.0 parts by weight of phospholipid, and 0.1 to 5.0 parts by weight of polyacetylene.

The red ginseng oil of the present invention may be contained in an amount of 0.0001 to 99.9 wt% (w / v) based on the total weight of the cosmetic composition, and preferably 0.01 to 10.0 wt% (w / v). When the amount of the red ginseng oil is less than the above range, the effect of improving skin wrinkles can not be obtained. If the amount of the red ginseng oil is more than the above range, the increase in the content of the red ginseng oil does not increase.

In a preferred embodiment of the present invention, the skin wrinkles may be caused by photoaging or natural aging, more preferably by photoaging, and more preferably by photoexposure by UV exposure, but are not limited thereto. In particular, the skin wrinkles are based on, but not limited to, MMP, preferably MMP-2 or MMP-9 expression or activity.

The cosmetic composition according to the present invention may further contain other ingredients such as the red ginseng oil, which can give a synergistic effect to the effect of the red ginseng oil, within a range that does not impair the desired effect of the present invention have. For example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, or carriers.

The cosmetic composition of the present invention is not particularly limited in its formulation and may be prepared into any formulation conventionally produced in the art, and examples thereof include softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, And may be formulated into such forms as essence, pack, body lotion, body cream, body oil, body essence, gel, emulsion, body cleanser, hair dye, shampoo or rinse.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component in the case of a powder or a spray. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component. For example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol , Benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and in the case of cleansing containing a surfactant, aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, A fatty acid amide ether sulfate, an alkylamidobetaine, an aliphatic alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a lanolin derivative, or an ethoxylated glycerol fatty acid such as isopentanoate, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, s Include Le can be used. Specifically, the softening water may contain 1.0 to 10.0% by weight of polyhydric alcohols such as propylene glycol and glycerin, and 0.05 to 2.0% by weight of surfactants such as polyethylene oleyl ether and polyoxyethylene hardened castor oil. The nutritional lotion and nutritional cream contain 5.0 to 20.0% by weight of an oil such as squalane, vaseline, octyldodecanol, and 3.0 to 15.0% by weight of a wax component such as cetanol, stearyl alcohol and beeswax in addition to red ginseng oil, And 5.0 to 30.0% by weight of polyhydric alcohols such as glycerin, propylene glycol and the like. The massage cream is prepared by adding 30.0 to 70.0% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate in addition to red ginseng oil, and the pack is peel off containing 5.0 to 20.0% by weight of polyvinyl alcohol. A pack or a general emulsion type cosmetic can be produced with a wash off pack containing 5.0 to 30.0% by weight of pigment such as kaolin, talc, zinc oxide, titanium dioxide and the like. In addition, the cosmetic composition of the present invention may further contain additives such as a preservative component, a moisturizer, an emollient, a surfactant, an organic or inorganic pigment, an organic powder, an ultraviolet absorbent, an antiseptic, a bactericide, an antioxidant, a plant extract, A blood circulation accelerator, a cold agent, purified water and the like may be further added as a blending component. The amount is preferably 0.001 to 5.0% by weight, more preferably 0.01 to 3.0% by weight based on the total weight of the composition.

In a specific embodiment of the present invention, in order to confirm the skin wrinkle-reducing effect of the red ginseng oil of the present invention, human fibroblasts were treated with MMP-2 and MMP -9 was examined for the effect of red ginseng oil. MMP-2 and MMP-9 are gelatine-degrading enzymes using gelatin as a substrate. It is known that MMP production is promoted by photo-aging, thereby accelerating the decomposition of skin fibers to induce wrinkles. The red ginseng oil of the present invention was found to significantly decrease the expression of MMP-2 and MMP-9, which are increased by UV (see Table 2). Accordingly, the red ginseng oil of the present invention is effective as a skin homeostasis maintenance and enzyme inhibitor, and thus can be usefully used in a cosmetic composition for improving skin wrinkles.

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing red ginseng oil as an active ingredient.

In a preferred embodiment of the present invention, the red ginseng oil of the present invention is extracted from red ginseng or red ginseng and is a nonpolar solvent soluble extract of red ginseng or red ginseng powder. As the nonpolar solvent, hexane, chloroform, dichloromethane, ethyl acetate or a mixed solvent thereof may be used, and hexane is preferable.

The red ginseng oil of the present invention thus extracted contains as main components fat, vegetable sterol, phospholipid and polyacetylene, preferably 70.0 to 90.0 parts by weight of fat, 0.1 to 15.0 parts by weight of phytosterol, 0.1 to 10.0 parts by weight of phospholipid, 0.1 to 5.0 parts by weight of polyacetylene.

The red ginseng oil of the present invention may be contained in an amount of 0.0001 to 99.9% by weight (w / v), preferably 0.01 to 10.0% by weight (w / v) based on the total weight of the pharmaceutical composition. When the amount of the red ginseng oil is less than the above range, the effect of improving skin wrinkles can not be obtained. When the amount of the red ginseng oil is more than the above range, no marked increase in the effect is observed.

The administration route of the pharmaceutical composition according to the present invention includes oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual, or rectal administration and is preferably oral or parenteral But is not limited thereto. The parenteral route includes subcutaneous, intradermal, intravenous, intramuscular, intralesional injection or infusion techniques.

The pharmaceutical composition of the present invention may contain, in addition to the red ginseng oil of the present invention, one or more active ingredients showing the same or similar functions. The compositions may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, oral preparations, external preparations, suppositories and sterilized injection solutions according to a conventional method.

Solid form preparations for oral administration include powders, granules, tablets, capsules, soft capsules, and the like. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . As the agent for parenteral administration, the external preparation such as powders, granules, tablets, capsules, sterilized aqueous solutions, liquid preparations, non-aqueous solutions, suspensions, emulsions, syrups, suppositories and aerosols, And may be formulated into a pharmaceutical composition for external application for cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasm. However, the present invention is not limited thereto. Herein, the ointment for skin external use may contain 50.0 to 97.0% by weight of petrolatum and 0.1 to 5.0% by weight of polyoxyethylene oleyl-ether phosphate other than red ginseng oil. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of suppository bases include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like.

Such compositions may additionally contain preservatives, stabilizers, wettable or emulsifying accelerators, adjuvants such as salts and / or buffers for the control of osmotic pressure, and other therapeutically useful substances and may be prepared by conventional methods of mixing, It can be formulated according to the coating method.

The pharmaceutical composition of the present invention may be variously varied depending on various factors including the age, weight, general health, sex, administration time, route of administration, rate of excretion, drug combination and severity of a specific disease.

When the pharmaceutical composition containing the red ginseng oil of the present invention as an active ingredient is formulated in a unit dose form, it is preferable that the red ginseng oil of the present invention is contained as an effective ingredient in a unit dose of about 0.01 to 1,500 mg, The dosage required for treatment is usually, but not limited to, about 1 to 500 mg per day depending on the frequency and intensity of administration, and in some patients a higher daily dose may be desirable.

 The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

The red ginseng oil of the present invention remarkably reduces the expression of MMP-2 and MMP-9, which are increased by photoaging due to UV exposure, thereby improving the skin wrinkles caused by MMP-2 or MMP-9 expression or activity Therefore, it can be effectively used as an active ingredient of a pharmaceutical composition for improving skin wrinkles.

The red ginseng oil according to the present invention has the effect of suppressing the expression of the substrate protease, which is increased in expression by stress and aging, thereby improving the skin wrinkle caused by the enzyme. Therefore, And the like.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following examples and experimental examples are provided only for illustrating the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

< Example  1> Preparation of red ginseng oil using red ginseng powder

60 mg of hexane was added to 1 kg of red ginseng powder and refluxed at 65 ° C for 60 minutes to extract lipid soluble components. The obtained filtrate was subjected to removal of hexane under reduced pressure to obtain a dark red ginseng oil.

< Example  2> Red ginseng  Manufacture of red ginseng oil

Red ginseng oil was prepared in the same manner as in <Example 1> except that red ginseng oil was obtained using red ginseng powder instead of red ginseng powder.

< Example  3> Red ginseng oil component analysis

The red ginseng oil components obtained in Example 1 and Example 2 were analyzed by high performance liquid chromatography (HPLC).

As a result, it was confirmed that red ginseng oil contained more than 81% of the fat component, and the plant sterol, phospholipid, polyacetylene and other components (Table 1).

weight% maintain Plant sterol Phospholipids Polyacetylene Etc Example 1 81.64 10.08 5.15 0.59 2.54 Example 2 81.59 9.62 5.46 0.61 2.72

< Experimental Example  1> Red ginseng oil MMP2  And MMP9  Confirming skin wrinkle improving activity through inhibition of expression

Human fibroblasts (ATCC 2076) were dispensed into a 60 mm dish at a density of 1 × 10 ⁶ cells using IMDM medium containing 10% bovine serum (Iscove's modified Dulbeco's medium; BRL, USA) And cultured at 37 ° C. Subsequently, the medium was washed twice with HBSS (Hank's Balanced Salt Solution) and irradiated with UV-A 6J in the presence of 2 ml of HBSS. After the irradiation, HBSS was removed and replaced with bovine serum-free IMDM. Then, red ginseng oil of the present invention was added and cultured at 37 ° C for 24 hours. Then, the cell culture medium was recovered and put into a 15 ml centrifuge filter device, and the protein was quantified after concentration until it became less than 100 μl. The sample was loaded on a 10% gelatin gel gel and subjected to SDS-PAGE at 125 V for 90 minutes. The gel was then incubated in a renaturing buffer for 1 hour and 30 minutes. After incubation for 30 min in a developing buffer, the cells were incubated overnight at 37 ° C. The cells were then stained with Coomassie blue to confirm the reaction.

As a result, it was confirmed that the red ginseng oil of the present invention has a wrinkle-reducing effect by decreasing the active amount of collagen, elastin degrading enzymes MMP-2 and MMp-9 in a concentration-dependent manner (Table 2).

sample MMP-9 MMP-2 Active type Inactive type Control group 30.06 68.04 93.00 UV-A 6J 100 100 100 UV-A 6J + 0.87 mg / ml of red ginseng oil of Example 2 83.85 91.18 96.08 UV-A 6J + 2.17 mg / ml of red ginseng oil of Example 2 81.29 83.53 99.00 UV-A 6J + Red ginseng oil of Example 2, 4.33 mg / ml 75.51 80.83 93.69 UV-A 6J + 8.67 mg / ml of red ginseng oil of Example 2 62.27 70.03 106.81

< Manufacturing example  1> Cosmetics  Preparation of composition

<1-1> Production of softening longevity

The softened lotion containing the red ginseng oil of the present invention as an active ingredient was prepared as shown in Table 3 below.

Raw material Content (parts by weight) <Example 1> Red ginseng oil 10.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<1-2> Preparation of nutritional cream

The nutritional cream containing the red ginseng oil of the present invention as an active ingredient was prepared in accordance with the composition shown in Table 4 below.

Raw material Content (parts by weight) <Example 1> Red ginseng oil 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

<1-3> Production of Lotion

Lotions containing the red ginseng oil of the present invention as an active ingredient were prepared according to the composition shown in Table 5 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Pro-type glycerin monostearate 1.8 FAGE -100 stearate 2.6 Sorbic acid sesquioleate 0.6 Squalene 4.8 Macadia oil 2 Jojoba oil 2 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Ethylparaben 0.1 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Ethylparaben 0.1 Propylparaben 0.1 1,3-butylene glycol 4 Methylparaben 0.1 Xanthan gum 0.1 glycerin 4 d-Pandenol 0.15 Allantoin 0.1 <Example 2> Red ginseng oil 3.5 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 pt 41891 0.1 p-H20 48.3

< Manufacturing example  2> Of the pharmaceutical composition  Produce

<2-1> Preparation of ointment for external skin

Skin ointment containing the red ginseng oil of the present invention as an active ingredient was prepared according to the composition of the following [Table 6].

Raw material Content (parts by weight) Purified water 100 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 <Example 1> Red ginseng oil 20 Caprylic capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Wax 4.0

<2-2> External application Patchy  Produce

Skin patches containing the red ginseng oil of the present invention as an active ingredient were prepared according to the composition shown in Table 7 below.

Raw material Content (parts by weight) <Example 2> Red ginseng oil 10.0 Hexylene glycol 20.0 Diethylamine 0.7 Polyacrylic acid 1.0 Sodium sulphate 0.1 Polyoxyethylene lauryl ether (E.0 = 9) 1.0 Polyhydroxyethylene cetyl stearyl ether 1.0 Viscous paraffin oil 2.5 Caprylic acid ester / capric acid ester (cetiol LC) 2.5 Polyethylene glycol 400 3.0 Deionized water 70

<2-3> Sanje  Produce

2 g of red ginseng oil of Example 2

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

<2-4> Preparation of tablets

100 mg of red ginseng oil of Example 2

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 2-5 > Preparation of capsules

100 mg of red ginseng oil of Example 1

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

&Lt; 2-6 >

1 g of red ginseng oil of Example 1

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

<2-7> Preparation of granules

150 mg of red ginseng oil of Example 2

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and the mixture was dried at 60 캜 to form granules, which were then filled in a capsule.

Claims (11)

Wherein the red ginseng oil comprises 70.0 to 90.0 parts by weight of a fat, 0.1 to 15.0 parts by weight of a plant sterol, 0.1 to 10.0 parts by weight of a phospholipid and 0.1 to 5.0 parts by weight of a red ginseng oil extracted with hexane as an active ingredient. A cosmetic composition for improving skin wrinkles.
The method according to claim 1,
Wherein the red ginseng oil is extracted from red ginseng or red ginseng.
delete delete delete The method according to claim 1,
The cosmetic composition may be any one selected from the group consisting of softening agents, astringent lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, body lotion, body cream, body oil, body essence, gel, emulsion and body cleanser &Lt; / RTI &gt;
Wherein the red ginseng oil comprises 70.0 to 90.0 parts by weight of a fat, 0.1 to 15.0 parts by weight of a plant sterol, 0.1 to 10.0 parts by weight of a phospholipid and 0.1 to 5.0 parts by weight of a red ginseng extract, Claims 1. A pharmaceutical composition for improving skin wrinkles comprising polylactylene in a weight part.
The method of claim 7,
Wherein the red ginseng oil is extracted from red ginseng or red ginseng.
delete delete delete