KR101527228B1 - Composition Containing Extracts of Acanthopanax senticosus - Google Patents

Abstract

Disclosed is a composition containing an astringent skin extract as an active ingredient. The composition has an effect of inhibiting the deposition of acne pigment and can be used in various fields in the field of cosmetics, food or medicine.

Acanthopanax sp. Extract, Acne, Pigmentation, External medicine, Cosmetics, Aftercare

Description

Composition Containing Extracts of Acanthopanax senticosus < RTI ID = 0.0 >

The present invention relates to a composition containing an extract of Aspergillus oryzae as an active ingredient.

In general, acne is caused by sebum excess associated with male hormone secretion. Specifically, sebum is generated by the male hormone and hyperkeratinization of the pores is induced, so that the entrance of the pore narrows or becomes obstructed in severe cases. When the pore opening becomes narrow or clogged, the sebaceous pores in the pores are prevented from being discharged to the outside, and microfeatures are formed. Propionibacterium acnes, an anaerobic bacteria living in the pores, have.

The inflammatory reaction causes erythema, tingling, or swelling, and in severe cases hyperpigmentation may occur. In addition, when the acne area is squeezed or pressed, the risk of acne scarring is increased as the cotton bum pops and the inflammation worsens or tissue damage occurs.

Such acne inflammation can be caused by various causes, for example, various additives contained in cosmetics may be left on the skin and cause acne inflammation. Skin inflammation may also be caused by sebum, sweat or ultraviolet rays emitted from the body.

In acne inflammation caused by various causes, research and development are concentrated on the treatment of inflammation itself. However, even if the inflammation is treated, the skin erythema or scar resulting therefrom remains, resulting in poor cosmetic results. Therefore, in the present invention, studies were conducted to inhibit or prevent pigmentation due to acne inflammation.

An object of one embodiment of the present invention is to provide a composition for inhibiting acne pigmentation.

Another object of the present invention is to provide a composition for aftercare for acne sequelae.

The composition according to one embodiment of the present invention is characterized by containing an extract of Aspergillus oryzae as an active ingredient.

The composition according to one embodiment of the present invention has an effect of inhibiting the deposition of acne pigment. As a result, it can be used in various fields such as cosmetics, food or medicine.

The composition according to the present invention contains Aspergillus oryzae extract as an active ingredient. The composition containing the extract of Aspergillus oryzae is recognized to be effective for preventing or suppressing acne pigmentation. In one embodiment, the composition according to the present invention is a composition for inhibiting an acne pigmentation deposition, which comprises an extract of Aspergillus oryzae as an active ingredient.

In the present invention, Acanthopanax senticosus is a deciduous shrub of Araliaceae, sometimes referred to as Acanthopanax senticosus. The height of the goose is about 2-3 m. Thin and long thorns are densely formed throughout the tree, and the folded leaves of the palm-like shape run diagonally. Among the Acanthopanax , Acantho means 'thorn tree', and 'panax' means 'thorn tree that regulates all kinds of diseases'. It is widely used for neuralgia, arthritis, hypertension, nervous breakdown, diabetes and tonic.

The goethite extract can be extracted by various conventional methods without any limitation. In one embodiment, the extract can be extracted with an organic solvent such as distilled water, C ₁ to C ₅ anhydrous or hydrated lower alcohols. For example, purified water is added to garlic extract and extracted at room temperature for 12 to 36 hours. The extract is allowed to settle the precipitate at a low temperature, followed by filtration to separate the filtrate. With activated carbon, the process of removing the color and odor of the filtrate can be further roughened.

The composition according to the present invention can be utilized in various fields without particular limitation, for example, it can be formulated as an external preparation for skin, specifically, in the form of a cosmetic composition or a pharmaceutical composition.

The composition for external application for skin according to the present invention may be in the form of a solution, suspension or emulsion in an oil or an aqueous medium, or may be formulated in the form of a dry powder which is dissolved in sterile, exothermic water before use. The water-in-oil type emulsion is prepared by emulsifying a mineral oil such as vegetable oil or liquid paraffin in an oil phase such as olive oil, natural acid phospholipids such as soybean lecithin and those derived from anhydride or fatty acid esters such as sorbitan monooleate, Such as ethylene sorbitol monooleate, are condensed with ethylene oxide as emulsifiers to emulsify the active ingredient.

In one embodiment, in the composition for external application for skin according to the present invention, the content of Rhizome extract may be 0.5 to 20% by weight, based on the total weight of the composition. When the content of the extract is less than 0.5% by weight, the effect of the control and improvement of the skin condition is insignificant. When the content of the extract is more than 20% by weight, the efficiency due to the addition of the extract is decreased and the formulation stability and other problems may be caused.

When the composition is formulated in the form of a cosmetic, it can be used for the purpose of preventing or improving acne pigmentation. It can also be used for the purpose of preventing or improving the occurrence of acne scars. The cosmetic formulation may be, for example, a cosmetic composition having a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic composition, , Creams, patches, or spray formulations. Further, in the respective formulations, other ingredients may be selected and mixed without difficulty by those skilled in the art depending on the kind of the other cosmetic composition or the purpose of use.

The present invention also provides a pharmaceutical composition comprising said composition. The pharmaceutical composition comprising the composition according to the present invention may be a composition for improving or inhibiting acne pigmentation and / or acne scars.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, soft capsules, powders, granules, powders, emulsions, syrups and pellets. . Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

In addition, the dosage of the active ingredient will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art. Typical doses are from 0.001 mg / kg / day to 2000 mg / kg / day, more specifically from 0.5 mg / kg / day to 1500 mg / kg / day.

In one embodiment, the present invention provides a composition for aftercare that contains an extract of Rhodiola cinerea. More specifically, the present invention provides a composition for an aftercare for comprehensively managing the after-effects such as pigmentation and / or scarring due to skin troubles caused by acne and improving the skin condition.

In another embodiment, in the composition for aftercare according to the present invention, the content of Rhizome extract may be 0.5 to 20% by weight, based on the total weight of the composition. When the content of the extract is less than 0.5% by weight, the effect of the control and improvement of the skin condition is insignificant. When the content of the extract is more than 20% by weight, the efficiency due to the addition of the extract is decreased and the formulation stability and other problems may be caused.

In the present invention, 'after care' means comprehensive after-care for managing and improving the skin condition. For example, if you enjoy swimming in beaches, rivers or valleys during the holiday season, or for a long time, such as tanning or tanning, your skin may become overexposed to ultraviolet light and cause inflammation. In addition, various additives such as sunscreen or oil applied to the skin may cause acne inflammation by remaining on the skin. In addition, skin troubles such as acne inflammation can be caused by various causes in daily life. After care is meant to collectively refer to the skin care and skin care management for improving the skin condition, including acne inflammation. In addition, the composition for aftercare is meant to encompass a composition for sticking, depositing or applying to the skin for aftercare.

In one embodiment, the present invention provides a food additive, a functional food, a health food or the like containing the composition. Specifically, the composition may be a composition for improving or suppressing acne pigment deposition.

The composition according to the present invention provides various types of food additives or functional foods containing them. It can be processed into fermented milk, cheese, yogurt, juice, probiotic agent and health supplement food including the above composition, and it can be used in various other food additives.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

[Example 1] Preparation of Acanthopanax senticosus extract

20 kg of purified water was added to 1 kg of goose root and extracted at 25 캜 for 24 hours. The extract was allowed to settle at 0 to 4 ° C for 24 hours, and then filtered through a 250 mesh filter. The activated carbon was used to remove the color and odor of the filtrate, and further subjected to a repetitive filtration process to prepare a yellowish white extract.

[Experimental Example 1] Cell culture conditions

Hamster sebocytes were extracted from the sebaceous glands of five-week-old male golden hamster ears. For specific extraction methods, see Sato et al. (J Invest Dermatol 2001, 117: 965-70). The extracted sebaceous cells were cultured in a culture flask so as to be 2.35 x 10 ⁴ / cm ² .

The culture medium was prepared by adding fetal bovine serum (JRH Bioscience, Tokyo, Japan) to DMEM / Ham's F12 medium (DMEM / Ham's F12 medium; (DMEM / F12) (Invitrogen, Carlsbad, CA) (Invitrogen) and 10 nM of rhEGF (recombinant human epidermal growth factor; Progen Biotechnik GmbH, Heidelberg, Germany).

Penicillin 100 mg / ml and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were used as antibiotics. Hamster sebaceous cells were attached to the culture flask for 24 hours and cultured at 37 ° C and 5% CO ₂ .

HaCaT, a human keratinocyte cell line, was prepared by adding 10% fetal bovine serum (JRH Bioscience, Tokyo, Japan) to DMEM medium (Invitrogen, Carlsbad, Calif.), Adding penicillin 100 mg / The cells were cultured in a medium supplemented with 100 mg / ml streptomycin (all from GIBCO, Milan, Italy) at 37 ° C and 5% CO 2.

Melan-a melanocyte melanocyte derived from C57BL / 6J mouse was cultured in RPMI-1640 (Invitrogen, Carlsbad, CA) with fetal bovine serum (JRH Bioscience, Tokyo, 10% CO ₂ (100 μl) was added to the medium supplemented with 200 nM of TPA (tetradecanoyl phorbol acetate), 100 mg / ml of penicillin and 100 mg / ml of streptomycin (all from GIBCO, Milan, Italy) Lt; / RTI >

[Experimental Example 2] In vitro evaluation of inhibition of acne inflammation

In order to evaluate effectively the inhibition of acne inflammation of Acanthopanax senticosus extract, we used in vitro inflammation model of environment similar to human acne lesion by using sebaceous gland cells and keratinocytes together. HaCaT keratinocytes and golden hamster sebaceous cells were inoculated in a 24 well culture plate at 3.75x10 ⁴ and 6.0x10 ⁴ , respectively. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. At this time, the medium was incubated with fetal bovine serum (JRH Bioscience, Tokyo, Japan) supplemented with DMEM / Ham's F12 medium (DMEM / Ham's F12 medium; (DMEM / F12) (Invitrogen, Carlsbad, CA) (Penicillin 100 mg / ml) and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were added to each well.

Day of linoleic acid with irritants in the last cell for causing acne inflammation (linoleic acid) 50 μM, arachidonic acid (arachidonic acid) 50 μM, dihydrotestosterone (dihydrotestosterone) 10 nM, acne bacteria (P. acnes) treated with 0.5% Respectively. At the same time, acne inflammation - inhibiting effect of stimulants was observed by treating the extract of. DMSO was used as a negative control and 100 nM isotretinoin, a widely used drug for acne treatment, was used as a positive control. (GM-CSF) (granulocyte macrophage colony-stimulating factor), IL-1α (interleukin-1alpha), and IL-1α, which are the inflammatory cytokines dissolved in the culture medium The concentrations of IL-8 (interleukin-8) and TNF-α (tumor necrosis factor-alpha) were measured in a 50 μl medium using a multiplex bead-based cytokine assay.

More specifically, the following procedure was carried out: The solution in the 96-well filter plate, which had previously been soaked with the wash buffer, was all sucked off using a vacuum pump. Next, the cell culture was reacted with an antibody-conjugated bead for 30 minutes. When the reaction was completed, a detection antibody was added and the reaction was further continued for 30 minutes. Then streptavidin-phycoerythrin (Streptavidin-PE) was added to each well and waited for 30 minutes. The unbound Streptavidin-PE was removed using wash buffer and the amount of beads attached to the cytokine was analyzed using a Bio-plex 200 device. The results of the analysis are shown in FIG. 1 (GM-CSF), FIG. 2 (IL-1α), FIG. 3 (IL-8) and FIG. 4 (TNF-α).

Referring to FIGS. 1 to 4, it was confirmed that the extract of A. rhizome according to the present invention has an effect of inhibiting inflammatory cytokines in a concentration-dependent manner.

[Experimental Example 3] In vitro evaluation of inhibition of acne-forming sebum production

In order to evaluate effectively the inhibitory effect of Acanthopanax senticosus extract on sebaceous glands, we used an in vitro model of environment similar to human acne lesion using sebaceous gland cells and keratinocytes together. HaCaT keratinocytes and golden hamster sebaceous cells were inoculated in a 24 well culture plate at 3.75x10 ⁴ and 6.0x10 ⁴ , respectively. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. At this time, the medium was incubated with fetal bovine serum (JRH Bioscience, Tokyo, Japan) supplemented with DMEM / Ham's F12 medium (DMEM / Ham's F12 medium; (DMEM / F12) (Invitrogen, Carlsbad, CA) (Penicillin 100 mg / ml) and streptomycin 100 mg / ml (all from GIBCO, Milan, Italy) were added to each well.

Day of linoleic acid with irritants in the last cell for causing acne inflammation (linoleic acid) 50 μM, arachidonic acid (arachidonic acid) 50 μM, dihydrotestosterone (dihydrotestosterone) 10 nM, acne bacteria (P. acnes) treated with 0.5% Respectively. At the same time, acne inflammation - inhibiting effect of stimulants was observed by treating the extract of. DMSO was used as a negative control and 100 nM isotretinoin, a widely used drug for acne treatment, was used as a positive control. The amount of triglyceride produced in the cells was measured using Oil Red O staining method after treatment with stimulus source and extracts.

More specifically, the following procedure was carried out: Cells washed with PBS were fixed with 3.7% formaldehyde solution for 30 minutes. The fixed cells were washed 3 times with PBS, once with 70% ethanol, and stained with 0.4% Oil Red O solution for 30 minutes. The stained cells were washed once with 70% ethanol and three times with PBS. Then, isopropanol dyed Oil Red O solution was re-dissolved and absorbance was measured at 520 nm using a spectrophotometer. The results of quantification are shown in Fig. Referring to FIG. 5, it can be seen that the extract of Rhodiola extract has an anti-sebum effect in a concentration-dependent manner.

[Experimental Example 4] In vitro evaluation of inhibition of acne pigment deposition

In order to efficiently evaluate the inhibitory effect of Acanthopanax senticosus extract on melanin - forming cells, an in vitro model of an environment similar to pigmentation induced by human acne inflammation was used. Melan-a melanin-forming cells were inoculated in a 24-well culture plate at 5.0 x 10 ⁴ cells / well. After inoculation, the cells were allowed to adhere to the bottom of the culture plate for one day. At this time, 10% of fetal bovine serum (JRH Bioscience, Tokyo, Japan) was added to RPMI-1640 (Invitrogen, Carlsbad, Calif.) And 200 nM of tetradecanoyl phorbol acetate (TPA), 100 mg of penicillin / ml, streptomycin 100 mg / ml (all from GIBCO, Milan, Italy).

One day later, 50 μM of linoleic acid, 50 μM of arachidonic acid, 10 nM of dihydrotestosterone, and 0.5% of acne ( P. acnes ) were treated as stimulants to cause acne pigmentation . And cultured for 5 days. Thereafter, the inhibitory effect of acne pigmentation on the stimulants was examined by treating the extracts of. DMSO was used as a negative control, and hydroquinone (1 μM) and kojic acid (200 ppm) were used as positive control groups, respectively. Hydroquinone is a specialty medicine used as a melamine inhibitor. In addition, kojic acid is recognized as a whitening effect and may be used as one of raw materials for a cosmetic composition. However, Kojisan may cause a strong irritation to the skin, and recently it has been reported that it may cause various cancers. After 5 days of treatment, the extract was treated with 1 N NaOH to dissolve the melanin, and the absorbance was measured at 405 nm using a spectrophotometer. The measurement results are shown in Fig.

Referring to FIG. 6, the Rhizopus japonicus extract according to the present invention has an effect of inhibiting pigment deposition in a concentration-dependent manner. In this experiment, the pigmentation inhibition effect was most excellent at a concentration of 250 ppm. Thus, the yellowish white extract according to the present invention exhibits excellent pigment inhibiting effect and further excellent whitening efficacy is recognized.

Hereinafter, formulation examples of the composition will be described, but the present invention is not intended to be limited thereto but is specifically described.

[Formulation Example 1] Preparation of soft capsules

A soft capsule filling liquid was prepared by mixing 80 mg of the extract of Echinochloa crus-galli, 9 mg of vitamin E, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of vegetable hardening oil, 4 mg of yellow bean, and 9 mg of lecithin and mixing them according to a conventional method . 400 mg per capsule was filled to prepare a soft capsule. Separately from the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin and 10 parts by weight of sorbitol solution, and filled with the filling solution to prepare a soft capsule containing 400 mg of the composition of the present invention .

[Formulation Example 2] Preparation of tablet

The mixture was mixed with 80 mg of Extract of Liliaceae, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose, granulated using a fluidized bed drier, and 6 mg of sugar ester was added Respectively. 504 mg of these compositions were tabletted by a conventional method to prepare tablets.

[Formulation Example 3] Preparation of Drinking Agent

After mixing 80 mg of extract, 30 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid and 25 g of liquid oligosaccharide, 300 ml of purified water was added to each bottle to make 200 ml each. And then sterilized at 130 DEG C for 4 to 5 seconds to prepare a beverage.

[Formulation Example 4] Preparation of granules

The extracts were prepared by mixing 80 mg of extract, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose and 550 mg of starch, forming into granules using a fluidized bed granulator,

[Formulation Example 5] Production of softening longevity (skin lotion)

A mixture of 255 mg of purified water, 0.3 mg of Ascorbic Acid Extract, 0.3 mg of butylene glycol, 6.0 mg of propylene glycol, 0.3 mg of carboxyvinyl polymer, 0.6 mg of phage-12 nonylphenyl ether, 1.2 mg of polysorbate 80, 30.0 mg of ethanol, mg, and antioxidants, coloring matters, and flavorings, the above components were added in the prescribed amounts according to a conventional method for preparing the softening agent, and uniformly mixed to prepare the desired softening agent.

[Formulation Example 6] Production of nutritional lotion (milk lotion)

(Seppic) (15 mg), glycerin (9.0 mg), butylene glycol (20 mg), and purified water (234 mg) , 9.0 mg of propylene glycol, 0.3 mg of carboxyvinyl polymer, 0.3 mg of triethanolamine and 0.6 mg of each of antiseptic, colorant and fragrance were added in the prescribed amounts according to a conventional method of producing a nutritional lotion, After mixing, the desired nutritional lotion was prepared.

[Formulation Example 7] Production of massage cream

70 mg of purified water, 0.3 mg of ascorbic acid extract, 30.0 mg of wax, 4.5 mg of polysorbate 60, 60 mg of castrated castor oil 6.0 mg, sorbitan sesquioleate 2.4 mg, liquid paraffin 120.0 mg, squalane 15.0 mg, Montana 202 : Seppic), 15.0 mg of glycerin, 12.0 mg of butylene glycol, 12.0 mg of propylene glycol, 0.6 mg of triethanolamine and a small amount of each of preservatives, coloring matters and perfumes according to a conventional method for producing a massage cream. The ingredients were added in the proportions presented and mixed uniformly, and the desired massage cream was prepared.

[Formulation Example 8] Preparation of pack

0.1 mg of allantoin, 6.0 mg of ethanol, 0.3 mg of fingi-12-nonylphenyl ether, 0.3 mg of polyvinylpyrrolidone, 0.1 mg of polyvinyl alcohol, 0.2 mg of sodium carboxymethylcellulose, 5.0 mg of glycerin, 0.3 mg of Solvate 60, and purified water were added to each small amount of the preservative, coloring matter and perfume, and the above components were added in the prescribed amounts according to a conventional method for producing a massage cream, uniformly mixed, .

[Formulation Example 9] Preparation of gel

0.1 mg of beta-1,3-glucan, 0.05 mg of sodium ethylenediaminetetraacetate, 5.0 mg of glycerin, 0.3 mg of carboxyvinyl polymer, 5.0 mg of ethanol, 0.5 mg of phage-60 hardened castor oil, 0.3 mg of triethanolamine , Purified water was added to each small amount of preservatives, coloring matters and flavorings, and the above components were added in the prescribed amounts according to a conventional method for preparing a pack, and uniformly mixed to prepare a desired pack.

[Formulation Example 10] Ointment in external preparation for skin

10 mg of betaine-1,3-glucan, 10.0 mg of beeswax, 5.0 mg of polysorbate 60, 2.0 mg of hydrogenated castor oil 2.0 mg, sorbitan sesquioleate 0.5 mg, petrolatum 5.0 mg, liquid paraffin 10.0 Purified water was added to each small amount of each of small amounts of mg, squalane 5.0 mg, shea butter 3.0 mg, caprylic / capric triglyceride 5.0 mg, glycerin 10 mg, propylene glycol 10.2 mg, triethanolamine 0.2 mg and preservative, dye, , And the above components were added in the prescribed proportions according to the conventional ointment preparation method and uniformly mixed to prepare the desired ointment agent.

 [Formulation Example 11] Preparation of drug (patch) for topical administration

(EO = 10 mg), sodium lauryl sulfate (0.1 mg), sodium lauryl sulfate (1.0 mg), beta-l, 3-glucan 3.0 mg, hexylene glycol 20.0 mg, diethylamine 0.7 mg, polyacrylic acid (Carbopol 934P) 9), 1.0 mg of polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000), 2.5 mg of viscous paraffin oil, 2.5 mg of caprylic acid ester / caproic acid ester (Cetiol LC) and 3.0 mg of polyethylene glycol 400 , Purified water was added, and the above ingredients were added in the prescribed amounts in accordance with the conventional method for producing a patch, and then mixed uniformly to prepare a desired patch.

Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

(GM-CSF), Figure 2 (IL-1 alpha), Figure 3 (IL-8), and Figure 4 (TNF- [alpha]) show that the inflammatory cytokine of the extract of Acanthopanax senticosus according to one embodiment of the present invention Inhibitory effect;

FIG. 5 is a graph showing an effect of inhibiting the production of sebum of a goat's squid extract according to an embodiment of the present invention;

FIG. 6 is a graph showing an effect of inhibiting acne pigment deposition on the extract of Rhizobia extract according to an embodiment of the present invention.

Claims (6)

A composition for inhibiting sebum formation comprising an extract of Aspergillus oryzae as an active ingredient. A composition for external application for skin comprising the composition according to claim 1. 3. The method of claim 2, The composition for external application for skin according to claim 1, wherein the content of the extract is from 0.5 to 20% by weight, based on the total weight of the composition. 3. The method of claim 2, Wherein the composition is a cosmetic formulation. delete A food additive or health food composition comprising the composition according to claim 1.

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