KR20180031486A - Functional composition for improving hair loss and hair growth comprising plant cell fermentation extract and functional cosmetic comprising the same - Google Patents

Abstract

The present invention relates to a functional composition for alleviating hair loss and aiding hair growth, which comprises: purified water; and two or more fermented plant extracts selected from the group consisting of Lonicera japonica (honeysuckle) flower, Eclipta prostrata, Citrus unshiu peel, Mentha arvensis, Polygonum multiflorum root, Biota orientalis leaf, Morus alba leaf, Houttuynia cordata, Glycine soja (soybean) seed, Ecklonia cava, and Perilla frutescens leaf. The functional composition of the present invention can be used to provide cosmetic products that show effects of: bringing a scalp into a healthy state through significantly alleviating scalp itchiness, dandruff, and pimple; reducing hair loss; thickening hair; and causing hair roots to grow.

Description

TECHNICAL FIELD The present invention relates to a composition for improving hair loss and hair growth comprising a plant cell complex fermentation extract and a functional cosmetic composition containing the same,

The present invention relates to a composition for improving hair loss and hair growth comprising a plant cell complex fermented extract and a functional cosmetic composition containing the same.

The causes of hair loss have been discussed in male or female organs such as hair follicle, hair follicle, hair follicle, bacterial growth, sebaceous secretion, genetic factors and aging. However, the definite cause of hair loss has not yet been clarified. Recently, people who are suffering from alopecia are increasing and the age group is lowering.

There are no clear effects on the treatment of alopecia, and there are some prescriptions for taking hair loss, such as diagnosis of neonatal volume in Donguibogam, massage of sesame seeds on hair loss site, And the method of stimulating specific blood circulation. However, it is known that there are many individual differences in the effect, and there is not yet reported a new drug substance which can be generally applied.

Studies have been conducted to obtain a composition for preventing hair loss or hair growth from a natural substance having safety as compared with a synthetic material. In the related art, Japanese Patent Application Laid-Open No. 2003-081861 discloses a hair- Korean Patent Laid-Open No. 10-2014-0091376 discloses that chrysanthemum extract has an activity of promoting the growth of human dermal papilla cells and human keratinocytes, thereby exhibiting an effect of improving hair loss and hair growth.

1. Japanese Unexamined Patent Application Publication No. 2003-081861 (Mar. 19, 2003) 2. Korean Patent Publication No. 10-2014-0091376 (July 21, 2014).

The present invention provides a cosmetic composition which contains a fermented extract of a specific plant to provide a cosmetic exhibiting an improved effect such as prevention of hair loss and promotion of hair growth with less side effects and stability.

However, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

The present invention provides a composition for improving hair loss and hair growth comprising a plant cell complex fermentation extract as an active ingredient. The plant cell complex fermented extracts can be used in various fields such as Lonicera Japonica (Honeysuckle Flower), Eclipta Prostrata, Citrus Unshiu Peel, Mentha Arvensis, Polygonum Multiflorum Root, Biota Orientalis Leaf, Morus Alba Leaf, Houttuynia Cordata, Glycine Soja (Soybean Seed), Ecklonia Cava and Perilla Frutescens Leaf. Includes fermented extracts of plants.

The present invention also provides cosmetics for hair, shampoo, soap, tonic, and scalp essence comprising the hair loss and hair growth improving functional composition.

The present invention also provides the use of the above-mentioned hair loss and hair growth improving functional composition as a pharmaceutical composition and as a food composition.

The present invention relates to a composition containing a fermented extract of a specific plant. The composition is useful for alleviating the progress of hair loss by promoting hair growth and healthy scalp growth by a plant cell complex fermentation extract, preventing hair loss / hair growth, It can be used in hair growth improving functional cosmetics.

More specifically, the present invention relates to a cosmetic composition comprising a plant cell complex fermentation extract according to the present invention, which is effective in improving scalp itching, dandruff and rashes, thereby changing the scalp to a healthy state, reducing hair loss, can do.

Before describing the present invention in detail, it is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention, which is defined solely by the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art unless otherwise stated.

Throughout this specification and claims, the word "comprise", "comprises", "comprising" means including a stated article, step or group of articles, and steps, , Step, or group of objects, or a group of steps.

On the contrary, the various embodiments of the present invention can be combined with any other embodiments as long as there is no clear counterpoint. Any feature that is specifically or advantageously indicated as being advantageous may be combined with any other feature or feature that is indicated as being preferred or advantageous. Hereinafter, embodiments of the present invention and effects thereof will be described with reference to the accompanying drawings.

The hair loss and hair growth improving functional composition according to an embodiment of the present invention includes the active ingredient of the processed plant including the purified water and the mixed plant cell complex and promotes hair growth and healthy scalp growth thereby alleviating the progress of hair loss And hair wool / hair growth and hair growth promoting hair loss and hair growth functional cosmetics can be used.

Herein, the meaning of the above-mentioned "active ingredient" means an ingredient that alone exhibits the desired activity or can exhibit activity together with a carrier which is itself inactive. In the present specification, the "plant cell complex fermentation extract" means that at least two kinds of fermented extracts obtained by extracting an active ingredient from each specific plant according to the present invention and fermenting the same are included. Other Unless otherwise defined in this specification, the meaning of the word is either national or international.

The functional composition for improving hair loss and hair growth according to one embodiment of the present invention is a plant cell complex fermentation extract of Lonicera Japonica (Honeysuckle Flower), Eclipta Prostrata, Citrus Unshiu Peel, Mentha Arvensis, Polygonum Multiflorum Root, Biota Orientalis Leaf, Morus Alba Leaf, Houttuynia Cordata, Glycine Soja (Soybean Seed), Ecklonia Cava, (Perilla Frutescens Leaf). ≪ / RTI >

The hair loss and hair growth improving functional composition according to the present invention comprises 42 to 75 wt% of the purified water and 25 to 58 wt% of the plant cell complex fermentation extract.

The plant cell complex fermentation extract according to the present invention contains 3 to 10 wt% of the total composition when the fermented extract of Ganoderma lucidum is included. When the extract contains fermented extract of Angelica keiskei, it is contained in an amount of 3 to 9 wt% with respect to the total composition. In addition, when the fermented extract of tangerine peel, the fermented extract of peppermint, and the fermented extract of Sasao are contained, the content is 3 to 8 wt%, respectively, relative to the total composition. In case of including the fermentation extract of the leaves of Cervus sp., The fermentation extract of the upper leaves, the fermentation extract of the perennial herb, and the fermentation extract of the soybean, 2 to 7 wt% of the total composition is included. In addition, when the fermented extract of the menthol and the fermented extract of the true lobule are contained, the content is 0.1 to 4 wt%, respectively, relative to the total composition.

The functional composition for improving hair loss and hair growth preferably contains 42 to 75 wt% of purified water, 3 to 10 wt% of fermentation extract of Ganoderma lucidum, 3 to 9 wt% of fermented extract of Hanshikucho, 3 to 8 wt% of fermented extract of tangerine peel 3 to 8 wt% of fermented extract of mint, 3 to 8 wt% of fermented extract of Sucrose, 2 to 7 wt% of Fermented Extract of Panax notoginseng, 2 to 7 wt% of Fermented Extract of Upper leaf, 2 to 7 wt% of fermentation extract of soybean, 2 to 7 wt% of fermentation extract of soybean, 0.1 to 4 wt% of fermentation extract of menthol, and 0.1 to 4 wt% of fermentation extract of japanese leaf.

The plant cell complex fermentation extract is a fermentation extract obtained by fermenting the extract of the active ingredient of the plant by the second anaerobic fermentation using the Bacillus bacteria and the complex Kimchi lactic acid bacteria after the first aerobic fermentation. Specific methods of the first oxic fermentation and the second anaerobic fermentation will be described later.

The functional composition for improving hair loss and hair growth according to an embodiment of the present invention may contain a compound or a plant extract known to have hair loss prevention or hair growth promoting effect so as to increase or reinforce hair loss prevention or hair growth promoting effect, And further include compounds and plant extracts which are generally used in the future for the production of a hair dye.

Examples of the compound or plant extract include progesterone and estrogen such as female hormone, green tea extract, finasteride, white porcelain extract, gossam extract, linoleic acid, gamarinolenic acid, jojoba oil, olive oil, sunflower oil, avocado oil, lecithin with soybean oil Ginseng extract, cinnabar extract, ginseng extract, ginseng extract, ginseng extract, bingo extract, ginseng extract, ginseng extract, tea extract, ginseng extract, licorice extract, , Hanoki extract, huushuo extract, hwangjeong extract, pantothenic acid choletel, di-panthenol, wenna extract, iris extract, hinokitiol, nicotinamide, elmentol, minoxidil, ginkgo extract, tocopheryl acetate, benzyl nicotinate, salicylic acid, , Ketoconazole and the like.

The compounds or plant extracts exemplified above may be incorporated into the hair loss preventing or hair growth improving functional composition of the present invention together with the plant cell complex fermentation extract.

The hair cosmetic composition containing the functional composition for improving hair loss and hair growth according to one embodiment of the present invention has remarkably improved scalp itchiness, dandruff and pimples so that the scalp is changed into a healthy state, hair loss is reduced, hair is thickened, .

Examples of hair cosmetics include hair shampoos, essences, tonics, treatments, soaps, nutrients, emulsions, lotions, creams (oil-in-water type, water-in-water type, multiphase), solutions, suspensions ), Anhydrous product (oil and glycol), gel, powder and the like, and the functional composition is contained in an amount of 0.05 to 100 wt% with respect to the whole cosmetics, and in the case of ampoule formulation, the functional composition is 100% ≪ / RTI >

The hair cosmetic composition may contain an acceptable carrier in hair cosmetic preparations in addition to plant cell complex fermentation extracts. Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, preservatives, wetting agents, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents, flavoring agents and diluents.

Specific compounds or compositions that can be used as the above-mentioned alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosifier, emulsion, stabilizer, ultraviolet screening agent, coloring agent, A person skilled in the art can select and use the appropriate compound or composition.

Examples of alcohols include water-soluble polyhydric alcohols such as higher alcohols, propylene glycol, 1,3-butylene glycol, glycerin, sorbitol and polyethylene glycol. Examples of the oils include avocado oil, palm oil, Examples of the preservative include ethylparaben and butylparaben. Examples of the moisturizing agent include hyaluronic acid, chondroitin sulfate, and pyrrolidonecarboxylic acid salt. Examples of the diluent include ethanol, isopropanol, and the like .

In addition, in the case of a shampoo containing a plant cell complex fermentation extract according to an embodiment of the present invention, the shampoo may be prepared by including an acceptable carrier in a shampoo formulation other than a plant cell complex fermentation extract. Examples of the carrier include surfactants, Emulsifiers, thickeners, hydrotropes, preservatives, pH adjusting agents, bactericides, anti-dandruff agents, tonics, moisturizers and the like.

The surfactant, the foam enhancer, the emulsifier, the thickener, the hydrotrope, the preservative, the pH adjuster, the bactericide, the anti-dandruff agent and the tonic agent may be those known in the art. There can be used Alcohol Ethoxy Sulfate (Sodium Lauryl Ethoxylated Sulfate), Alkyl Sulfate, Acyl Sarsosinate, Acyl Grutaminate, Phosphoric Acid, Sucrose Ester and the like. Sodium hydroxide, sodium starch, starch, and the like can be used. As the perfume agent, ethyl alcohol, butyl alcohol, propylene glycol and the like can be used. As the humectant, glycerin Etc. may be used.

In the case of a soap comprising a plant cell complex fermentation extract according to an embodiment of the present invention, the soap may be prepared by incorporating a plant cell complex fermentation extract on a soap base, and may include a skin moisturizer, an emulsifier, .

Examples of the soap base material include vegetable oils such as palm oil, palm oil, soybean oil, perm free oil, olive oil and palm kernel oil or animal fats such as tallow, lard, sunshine and fish oil. Examples of the skin moisturizers include glycerin, erythritol, polyethylene glycol , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like. Examples of the emulsifier include natural oils, wax fatty alcohols, hydrocarbons, May be used. As the water softening agent, tetrasodium ethylenediate and the like may be used.

The soap composition of the present invention may further contain, as an additive, an antibacterial agent, a dispersant, a foam inhibitor, a solvent, a scouring inhibitor, a corrosion inhibitor, a fragrance, a dye, a sequestering agent, an antioxidant and an antiseptic.

The hair loss and hair growth improving functional composition according to one embodiment of the present invention can be used not only in cosmetics but also as a pharmaceutical composition and a food composition added to foods or medicines.

 The composition for improving hair loss and hair growth of the present invention can be used as a pharmaceutical composition in the form of pharmaceutical preparations such as creams, lotions, ointments (semi-solid external medicine), and the like, including pharmaceutically acceptable carriers, excipients, Gels, pastes, transdermal preparations (TTS) (e.g., patches, bandages, etc.), and the like. The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (e.g. corn starch, potato starch, etc.), cellulose, derivatives thereof (e.g. sodium carboxymethylcellulose, ethylcellulose, etc.), malt, gelatin, talc, (E.g., peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g., propylene glycol, glycerin, etc.), alginic acid, emulsifiers (e.g., TWEENS), wetting agents (For example, sodium lauryl sulfate), a coloring agent, a flavoring agent, a stabilizer, an antioxidant, a preservative, water, a saline solution, and a phosphate buffer solution. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, suspending agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropyl methyl cellulose, sodium alginate, polyvinyl pyrrolidone, .

The pharmaceutical compositions of the present invention may be administered orally or parenterally, and preferably topically. The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

In another specific embodiment, the composition of the present invention can be used as a food composition. For example, health supplements, special nutritional supplement foods, functional beverages, and the like. When the composition for improving hair loss and hair growth of the present invention is used as a food composition, it may contain sweetening agent, flavoring agent, physiologically active ingredient, minerals and the like in addition to its effective ingredient.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners. Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition. Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

If the composition of the functional hair restoration and hair growth improving composition and the functional cosmetic composition containing the same is satisfied, it can be stored for a long time without being preserved after the fermentation of the plant cell complex fermentation extract. If the composition is out of the above range, Etc., the desired fermentation products and efficacy may not be exhibited or undesired products may appear, and antimicrobial and antioxidant capacity may be lowered.

The method for preparing a plant-cell complex fermentation extract according to the present invention comprises culturing a microorganism inoculated and cultivated (S10), extracting an effective ingredient of the plant to obtain a plant extract And a fermentation step (S30) of fermenting the extracted plant extract with the cultured microorganism to obtain a fermented extract of the plant.

The step (S10) of culturing according to an embodiment of the present invention is a step of inoculating a medium with a microorganism including Bacillus and a compound Kimchi lactic acid bacterium and culturing the seed microorganism, wherein the microorganism to be used in the fermentation step (S30) .

More specifically, Bacillus subtillis and complex Kimchi lactic acid bacteria are inoculated into a heat-sterilized medium and cultured at 37 to 46 DEG C for 15 to 45 days. The cultured microorganism is lyophilized and powdered so that it can be used in a fermentation step (S30) to be described later.

The extracting step (S20) according to an embodiment of the present invention is a step of obtaining a plant extract by extracting an effective ingredient of a specific plant and obtaining a substance to be fermented in a fermentation step (S30) to be described later. The plants may be selected from the group consisting of Lonicera Japonica (Honeysuckle Flower), Eclipta Prostrata, Citrus Unshiu Peel, Mentha Arvensis, Polygonum Multiflorum Root, Biota Orientalis Leaf, And at least two plants selected from the group consisting of Morus Alba Leaf, Houttuynia Cordata, Glycine Soja (Soybean Seed), Ecklonia Cava and Perilla Frutescens Leaf .

More specifically, the plants are washed, dried and crushed, respectively, and then immersed in a solvent to obtain a plant extract. As the solvent, water containing distilled water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, or a mixed solvent thereof may be used.

The extraction means includes a concentrated liquid extract or a solid extract from which the extraction solvent has been removed. As long as it is extracted through the step of immersing the extraction target in the extraction solvent, the extraction method can be selected from the group consisting of cold, reflux, Any method such as radiation can be applied. Preferably, the plant extract can be obtained by immersion at 27 to 45 캜 for 15 to 90 days using an ethanol solution. The plant extracts obtained can also be used after sterilization.

The fermentation step (S30) according to an embodiment of the present invention is characterized in that the plant extract obtained in the extraction step (S20) is fermented (pH 5.0 to 7.5) using the cultured microorganism obtained in the cultivation step (S10) (S31) in which saccharides and carbon sources are added to the plant extract to aerobic fermentation, and a secondary fermentation step (S32) in which the culture microorganism is introduced into the primary fermentation and anaerobically fermented do.

Fermentation is a process of decomposing organic matter by enzymes secreted by microorganisms, which means that microorganisms produce substances useful in the process of obtaining energy. The culture microorganisms used in the fermentation step (S30) according to an embodiment of the present invention decompose sugars such as glucose or lactose to produce organic acids such as lactic acid and acetic acid, and enzymes such as amylase, cellulase, lipase, To facilitate penetration of the skin through the low molecular weight of macromolecules in the food extract.

The primary fermentation step (S31) according to an embodiment of the present invention is a step of adding saccharides and carbon sources to the plant extract extracted by the extraction step (S20) and fermenting the plant to natural exhalation, more specifically, The plant extract is added and 0.003 to 0.7 wt% of a saccharide and a carbon source is added to the mass of the plant extract and the mixture is fermented at a temperature of 17 to 32 ° C for 12 to 36 hours. After the first fermentation, pressure can be applied to bring the fermentation into a vacuum state, and the subsequent second fermentation can proceed.

The secondary fermentation step (S32) according to an embodiment of the present invention is a step of putting the culturing microorganism into a primary fermentation product fermented by the primary fermentation step (S31) and anaerobically fermenting the same, more specifically, Adding 0.05 to 6 wt% of Bacillus subtilis and complex Kimchi lactic acid bacteria cultivated in the fermented product to the mass of the first fermentation product and fermenting the mixture at a temperature of from 32 to 54 DEG C for 72 to 120 hours under vacuum to obtain a fermented extract of the plant to be.

The fermentation step (S30) according to an embodiment of the present invention may further include an aging step (S33) for aging the fermented extract of the plant after the secondary fermentation step (S32). More specifically, in the aging step (S33), the fermentation extract and the suspension obtained in the second fermentation step (S32) are separated by filtration and aged for one month or longer, whereby the aged fermented extract can be produced.

The functional composition for improving hair loss and hair growth according to an embodiment of the present invention can be prepared by including a mixing step (S40) of mixing the fermented extract of the plant with the purified water at a specific ratio. The complex fermented extracts of plant cells are composed of Lonicera Japonica (Honeysuckle Flower), Eclipta Prostrata, Citrus Unshiu Peel, Mentha Arvensis, Polygonum Multiflorum Root, Biota Orientalis Leaf, Morus Alba Leaf, Houttuynia Cordata, Glycine Soja (Soybean Seed), Ecklonia Cava and Perilla Frutescens Leaf were prepared, .

Preferably, the purified water is contained in an amount of 42 to 75 wt%, the fermentation extract of Ganoderma lucidum is 3 to 10 wt%, the fermentation extract of Hansikcho is 3 to 9 wt%, the fermentation extract of tangerine peel is 3 to 8 wt% 3 to 8 wt% of fermented extract of Sasa fungus, 3 to 8 wt% of fermented extract of Sasa folium, 2 to 7 wt% of Fermented Extract of Panax notoginosa leaf, 2 to 7 wt% of Fermented Extract of Upper leaf, % Of soybean, 2 to 7 wt% of soybean fermentation extract, 0.1 to 4 wt% of fermented extract of ganoderma lucidum, and 0.1 to 4 wt% of fermented extract of japanese lobule to produce a functional composition for improving hair loss and hair growth.

The functional hair cosmetic composition for improving hair loss and hair growth according to an embodiment of the present invention may be obtained by obtaining a complex fermentation extract of plant cells through the culturing step (S10), the extraction step (S20) and the fermentation step (S30) And a formulation step (S50) of obtaining a functional composition through step S40 and mixing it with the components constituting the other cosmetic products to prepare a cosmetic product of the desired formulation.

The formulation step S50 according to an embodiment of the present invention is a step of mixing cosmetic ingredients such as shampoos, essences, tonics, treatments, and nutrients by mixing the functional composition and other cosmetic ingredients. Further, the obtained fermented extract of the plant can be formulated using a sterilized filter (0.45 to 0.2 mu m).

(1) sensory evaluation - hair loss and hair growth improvement test

A shampoo containing the functional composition prepared according to the present invention was prepared and subjected to a clinical test for 7 months. Using the shampoo thus prepared, hair was observed to be improved, including hair removal prevention effect and hair growth promoting effect once a day after cleansing and care. Clinical tests were conducted on twelve women in their 40s and 70s, and eight men in total. The evaluation items were evaluated by the reduction of hair loss and the promotion of hair growth, and evaluated in terms of visual satisfaction and / or satisfaction / satisfaction / normal / insufficient / insufficient in five stages. The average values are shown in Table 1 below.

Evaluation contents Very satisfied
(5) satisfied
(4) usually
(3) Inadequate
(2) Very inadequate
(One) result Reduction of hair loss 9 7 3 One - 4.20 Promoting hair growth 8 6 4 2 - 4.00

As a result of checking the scalp and hair condition after using the shampoo for 7 months, it was found that the scalp was healthy and the scalp was itchy, dandruff and pimple, and the scalp became healthy, hair loss was reduced, hair thickened and hair root was grown.

(2) Evaluation of hair loss prevention and hair growth improvement

In order to confirm the hair loss prevention effect and hair growth effect of the plant cell complex fermentation extract according to the present invention, the inhibitory activity of 5α-reductase, which is known to promote dehydration by producing dihydrootestosterone, and the production inhibition rate of DHT .

3 to 10 wt% of a fermented extract of Ganoderma lucidum, 3 to 9 wt% of a fermented extract of Hansuksikcho, 3 to 8 wt% of a fermented extract of tangerine peel, a fermented extract of peppermint, a fermented extract of Sasao, The fermented extracts of the extracts, the upper leaves, the fermented extracts of the fermented sea mustard and the fermented extracts of the soybean were respectively contained in a range of 0.1 to 4 wt% Were prepared as plant cell combined fermentation extract samples (Preparation Examples 1 to 5).

5α-reductase was obtained from liver of Sprague-Dawley rats aged 8 weeks. Proteins were quantified by Bradford method and prepared as protein suspension. 30 μl of the suspension of 5α-reductase was added to 60 μl of the enzyme reaction solution (50 mM Na ₂ HPO ₄ (pH 6.8), 25 mM KCl, 500 mM NADPH) and 10 μl of the sample was added thereto, 100 mu l. At this time, radioactive labeled testosterone (3H-Testosterone) was used as the reaction substrate of 5α-reductase.

The reaction solution was allowed to stand in a 37 ° C thermostat for 20 minutes and reacted. The reaction solvent was removed by evaporation. The residue was dissolved in 20 μl of chloroform, spotted on a TLC plate, and eluted in a TLC chamber for 30 minutes in a developing solvent (toluene: acetone = 4: 1). The developed plate was exposed to a hyperfilm for 3 days, and the sensitized area was quantitatively analyzed with a densitometer.

The inhibitory activity (%) against 5α-reductase was evaluated using the following equation (1), and the results are shown in Table 2 below.

[Equation 1]

Activity inhibition (%) against 5α-reductase = 100 (A-B / A)

A: Conversion rate of testosterone to dihydrotestosterone

B: Conversion rate of testosterone to dihydrotestosterone when adding sample

Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Inhibition rate (%) 75 75 82 81 77

The 5α-reductase inhibitory activity of the plant-cell complex fermented extracts of Preparation Examples 1 to 5 was 75% or more at 100 μg / mL.

Meanwhile, testosterone, a male hormone, binds to the 5α-reductase enzyme in the body to produce dihydroxytestosterone (hereinafter referred to as "DHT"). This DHT binds to the androgen receptor of hair follicle, Which has a dominant influence on the inhibition. Therefore, we examined the effect of preventing hair loss through DHT production inhibition measurement.

6 g of liver tissue of SD rats was homogenized with 12 ml of PBS, treated with 1 mg / ml of the plant cell complex fermentation extract of the present invention in 1 ml of the homogenate, and then reacted at 37 ° C for 15 minutes. After 15 minutes, 1 ml of extraction buffer (50 mM Na2HPO4, 0.25 M sucrose) was added to the homogenate and the mixture was vortexed. After centrifugation at 4 ° C and 1,500 g, the supernatant was taken and used as a sample.

To measure the degree of DHT production, an ELISA kit for DHT (Uscn Life Science, China) was used. 100 쨉 l of the standard and the sample were dispensed into the enclosed 96-well plate, followed by reaction at 37 캜 for 2 hours. After 2 hours, the solution was removed, and 100 쨉 l of detection reagent A was added thereto. After 1 hour of reaction at 37 ° C, the cells were washed three times with wash buffer. 100 ㎕ of detection reagent B was dispensed and reacted at 37 캜 for 1 hour. After the reaction was completed, the supernatant was removed and washed with wash buffer 5 times. Subsequently, 90 μl of the substrate solution was added, the plate was wrapped with a foil, and the plate was reacted at 37 ° C for 15 minutes. 50 μl of stop solution was added to terminate the reaction and the absorbance was measured at 450 nm.

The inhibition rate (%) of DHT formation was measured using the absorbance of the sample solution added and the no-added solution using the following equation (2).

&Quot; (2) "

Inhibition rate (%) = (1 - absorbance of sample addition group / absorbance of no addition group) x 100

Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Inhibition rate (%) 75 75 72 71 80

As a result of measuring inhibition rate of DHT production, the combined plant cell fermentation extracts of Preparation Examples 1 to 5 showed a high inhibition rate of 70% or more at 100 μg / mL.

The features, structures, effects, and the like illustrated in the above-described embodiments can be combined and modified in other embodiments by those skilled in the art to which the embodiments belong. Therefore, it should be understood that the present invention is not limited to these combinations and modifications.

Claims (11)

A functional composition for improving hair loss and hair growth, comprising a plant cell complex fermentation extract as an active ingredient.
The method according to claim 1,
The plant cell complex fermented extracts can be used in various fields such as Lonicera Japonica (Honeysuckle Flower), Eclipta Prostrata, Citrus Unshiu Peel, Mentha Arvensis, Polygonum Multiflorum Root, Biota Orientalis Leaf, Morus Alba Leaf, Houttuynia Cordata, Glycine Soja (Soybean Seed), Ecklonia Cava and Perilla Frutescens Leaf. A functional composition for improving hair loss and hair growth comprising a fermented extract of a plant.
3. The method of claim 2,
Wherein the plant cell complex fermentation extract is contained in an amount of 25 to 58 wt%.
The method of claim 3,
Wherein the plant cell complex fermentation extract comprises 3 to 10 wt% of the fermentation extract of Ganoderma lucidum, 3 to 9 wt% of the fermented extract of the mascarpone, and the fermented extract of the tangerine peel, the fermented extract of the peppermint, Each containing 3 to 8 wt% of a fermented extract of the above-mentioned leaves, the fermentation extract of the above leaf, the fermentation extract of the above fermented extract, and the fermentation extract of the soybean, respectively, And 0.1 to 4 wt%, respectively, of the fermented extract of the true lobular lobes.
A hair cosmetic composition comprising the hair loss and hair growth improving functional composition of claim 1.
A shampoo comprising the hair loss and hair growth improving functional composition of claim 1.
A soap comprising the hair loss and hair growth improving functional composition of claim 1.
A tonic and scalp essence comprising the hair loss and hair growth improving functional composition of claim 1.
The hair loss and hair growth improving functional composition of claim 1, which is a pharmaceutical composition.
The hair loss and hair growth improving functional composition of claim 1, which is a food composition.
A culture step of inoculating Bacillus subtillis and a complex Kimchi lactic acid bacterium into a heat-sterilized medium and culturing the seed culture;
Eucalyptus leaf, Citrus Unshiu Peel, Mentha Arvensis, Polygonum Multiflorum Root, Biota Orientalis Leaf, Morus Alba leaf, Extracting effective components of two or more plants selected from the group consisting of Leaf, Houttuynia Cordata, Glycine Soja (Soybean Seed), Ecklonia Cava and Perilla Frutescens Leaf An extracting step of obtaining a plant extract;
A fermentation step of fermenting the plant extract with the culturing microorganism to obtain a fermented extract of the plant; And
And mixing the purified water with a fermented extract of the plant.