KR102611051B1 - Manufacturing method for cultivation of moss and moss cultivation kit for forest recovery thereof - Google Patents

Abstract

The present invention relates to a method for cultivating moss spores and a moss cultivation kit for forest restoration using the same. It relates to a method for cultivating moss spores that can restore forests by artificially cultivating them, making them dormant, and then spraying them on lost forests when a disaster occurs. .
The moss spore culture method according to the present invention includes a first step of cutting the sporangia into 10 to 30 mm; A second step of drying the cut sporangia at 60 to 75° C. for 24 hours and then crushing the dried sporangia; A third step of immersing the crushed sporangia in a spore culture medium and then making them dormant; A fourth step of immersing the dormant sporangia in a plant hormone solution for 24 hours; It is characterized in that it is manufactured including a fifth step of mixing the soaked sporangia with nutrient solution and adhesive solution and spraying it.

Description

Moss spore culture method and moss spore culture kit for forest recovery using the same {MANUFACTURING METHOD FOR CULTIVATION OF MOSS AND MOSS CULTIVATION KIT FOR FOREST RECOVERY THEREOF}

The present invention relates to a method for cultivating moss spores and a moss cultivation kit for forest restoration using the same. It relates to a method for cultivating moss spores that can restore forests by artificially cultivating them, making them dormant, and then spraying them on lost forests when a disaster occurs. .

Moss is a small, soft plant about 1 to 10 cm in size that belongs to the lichen or genus family. It grows in tangled groups in moist, shady places. In general, the distinction between leaves and stems is not clear, and it grows on old trees, rocks, and wetlands.

Currently, 16,500 species of mosses are known, of which two-thirds are in the phylum Moss and the remainder are hornworts and umbrella mosses. Moss is considered the most suitable plant for terraforming such as Mars due to its ability to survive and expand with just a little water. Moss does not have a cuticle layer that filters out foreign substances such as leaves, so it purifies more fine dust than trees of the same area.

In general, forest restoration or general greening of open fields is done by sowing or planting various types of plants. Weeds become dominant before the sown seeds germinate, causing the planted plants to die, or the soil is damaged by rainfall or wind. It is difficult to expect effective greening due to problems such as erosion, seed loss, and drying. Therefore, an alternative method is to conduct greening using moss. Once moss is established, it has strong self-reliance, so even if the leaves dry out due to drought, they will bloom again in full green color when exposed to dew or rain. Effective greening work can be carried out by applying the tenacious survival power of these moss.

However, in the case of moss, in the case of surfaces formed by structures such as sloping slopes, cut rock, cement structures, artificial rocks, and plastic surfaces, which are different from general open fields, not only are the structural surfaces slippery, but moss is a plant that proliferates through spores. Therefore, there is a problem in that cultivation is not easy because it cannot be fixed with roots.

Most conventional forest restoration techniques are labor-intensive, and commercial use of moss is very rare. Therefore, the present invention provides a moss spore culture method that can very efficiently reduce the cost, time, and manpower required for forest restoration, and provides a forest restoration kit using the same.

Korean Patent Publication No. 10-2011-0035626

The present invention was devised to solve the above problems, and the object of the present invention is to provide a method for cultivating moss spores that can restore forests by artificially cultivating moss, making it dormant, and then spraying it on forests that have been lost when a disaster occurs. will be.

The technical problems to be solved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the description below. You will be able to.

The moss spore culture method according to the present invention,

A first step of cutting the sporangia into 10 to 30 mm;

A second step of drying the cut sporangia at 60 to 75° C. for 24 hours and then crushing the dried sporangia;

A third step of immersing the crushed sporangia in a spore culture medium and then making them dormant;

A fourth step of immersing the dormant sporangia in a plant hormone solution for 24 hours;

It is characterized in that it is manufactured including a fifth step of mixing the soaked sporangia with nutrient solution and adhesive solution and spraying it.

By solving the above problem, the present invention can identify the pre-growth stage and spore generation stage of moss.

In addition, the present invention can restore forests by artificially cultivating moss spores, making them dormant, and spraying them on forests that have been lost when a disaster occurs.

1 is a flowchart showing the moss spore culture method of the present invention.
Figure 2 is a photograph of the cultivation and culture medium of spores prepared according to the present invention.
Figure 3 is a photograph before performing the second step after cutting the sporangia in the first step, according to an embodiment of the present invention.
Figure 4 is a photograph of samples collected from a disaster area to spread moss spores according to an embodiment of the present invention.
Figure 5 is a photograph showing 10 weeks after samples were left in a disaster area to spread moss spores, according to an embodiment of the present invention.
Figure 6 is a photograph taken 10 weeks after spraying moss spores prepared according to the present invention to the disaster area sample of Figure 5, according to an embodiment of the present invention.
Figure 7 is a photograph taken 14 weeks after spraying moss spores prepared according to the present invention to the disaster area sample of Figure 5, according to an embodiment of the present invention.
Figure 8 is a photograph of the 3rd stage spore culture solution (left), the 5th stage nutrient solution and adhesive solution (middle), and the 4th stage plant hormone solution (right), according to an embodiment of the present invention.

The terms used in this specification will be briefly explained, and the present invention will be described in detail.

The terms used in the present invention are general terms that are currently widely used as much as possible while considering the function in the present invention, but this may vary depending on the intention or precedent of a person working in the art, the emergence of new technology, etc. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than simply the name of the term.

When a part in the entire specification is said to “include” a certain element, this means that it does not exclude other elements but may further include other elements, unless specifically stated to the contrary.

Below, with reference to the attached drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

Specific details, including the problem to be solved by the present invention, the means for solving the problem, and the effect of the invention, are included in the examples and drawings described below. The advantages and features of the present invention and methods for achieving them will become clear by referring to the embodiments described in detail below along with the accompanying drawings.

Hereinafter, the present invention will be described in more detail with reference to the attached drawings.

The moss spore culture method of the present invention is preferably carried out by the following steps, as shown in Figure 1.

First, in the first step (S10), the sporangia are cut to 10 to 30 mm. More specifically, the sporangia are preferably pine moss, rattail moss, or roof moss. In addition, it is preferable to cut the sporangia to 10 to 30 mm, and the part outside the upper part of the sporangia only serves as a support for supporting the sporangia and does not contain spores, so it is performed under the above conditions.

Next, in the second step (S20), the cut sporangia are dried at 60 to 75 ° C. for 24 hours and the dried sporangia are crushed. More specifically, when the sporangia cut in the first step (S10) are dried at less than 60° C. or for less than 24 hours, drying is minimal, and when the sporangia are dried at more than 75° C. or for more than 24 hours, deformation of the spores occurs. Alternatively, it is preferable to dry for a long time under the above conditions at medium temperature because it causes death.

Next, in the third step (S30), the crushed sporangia are immersed in a spore culture medium and then become dormant.

The spore culture medium is a liquid used to culture spores and make them dormant so as not to germinate. More specifically, in the third step (S30), the spore culture medium preferably has a pH of 8 to 9. In general, in the case of forest disaster areas, the soil is acidic, so the spore culture can be neutralized by adjusting the spore culture to an appropriate base and spraying it. Therefore, it is desirable to set the pH to the above conditions with slightly alkaline properties.

Additionally, the electrical conductivity of the spore culture medium is preferably 0.5 to 1.0 mS/cm. More specifically, if the electrical conductivity of the spore culture is higher than the standard value, it becomes difficult to absorb moisture, cell growth slows down, and differentiation may be disrupted due to thick cell walls, so it is preferable to carry out the procedure under the above conditions.

Next, in the fourth step (S40), the dormant sporangia are immersed in a plant hormone solution for 24 hours.

The plant hormone solution is a liquid that promotes spore germination and the entire moss growth cycle. More specifically, in the fourth step (S40), the plant hormone solution is preferably mixed with equal amounts of abscisic acid, gibberellin, and auxin. . More specifically, the plant hormone solution is preferably mixed at 0.00005% (w/w) by mixing 0.5 mg of the abscisic acid, gibberellin and auxin mixture per 1 L of solvent.

[Table 1] below shows the results of the elongation experiment of rattail moss compared to the case where the concentration of the plant hormone solution was 0.5 mg/L, and [Table 2] compared the case where the concentration of the plant hormone solution was 2.0 mg/L. When 0.5 mg/L of plant hormone solution was immersed in rattail moss, as shown in [Table 1], the average was 1.1 mm after 30 days, 12.4 mm after 45 days, and 12.4 mm after 90 days. It was confirmed that the average was 23.9 mm. In addition, 2.0 mg/L of plant hormone solution was immersed in rattail moss, and as shown in [Table 2], the average was 1.9 mm after 30 days, 7.9 mm after 45 days, and 7.9 mm after 90 days. It was confirmed that the average was 16.7 mm.

In this rattail moss elongation experiment, the initial growth of moss immersed in 0.5 mg/L plant hormone solution was weak, but it was confirmed to be superior to moss immersed in 2.0 mg/L plant hormone solution after 45 days. As days passed, the moss elongation rate showed a growth rate of about 140%.

elapsed date Rattail Moss Height (mm) 1 repetition 2 repetitions 3 repetitions average 30 days 1.1 One 1.2 1.1 45 days 12.2 11.7 13.3 12.4 90 days 23 27.2 21.6 23.9

elapsed date Rattail Moss Height (mm) 1 repetition 2 repetitions 3 repetitions average 30 days 2. 2.2 1.4 1.9 45 days 9.1 8.8 5.9 7.9 90 days 21.2 19.1 9.7 16.7

In addition, by immersing the dormant sporangia in the plant hormone solution for 24 hours, the dormancy of the spores is broken before spraying, and at the same time, it is possible to have a faster and higher germination rate than when spraying in a disaster area.

Next, in the fifth step (S50), the submerged sporangia are mixed with nutrient solution and adhesive solution and sprayed.

The nutrient solution supplies basic nutrients after adhesion and germination of the moss spores when spraying the prepared moss spores. In addition, the adhesive helps to adsorb well without being easily swept away even under uncontrollable external conditions in a disaster environment and facilitates initial spore differentiation.

In the fifth step, the nutrient solution is prepared by mixing calcium nitrate (Ca(NO ₃ ) ₂ ), monobasic potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate aqueous solution (MgSO ₄ ·7H ₂ O), and medium. More specifically, the medium is preferably one selected from agar, alginate, carboxymethyl cellulose (CMC), or superabsorbent polymer (SAP). The nutrient solution was composed of calcium nitrate (Ca(NO ₃ ) ₂ ), potassium monobasic phosphate (KH ₂ PO ₄ ), magnesium sulfate aqueous solution (MgSO ₄ ·7H ₂ O) and medium to facilitate spore differentiation. It was adjusted based on the composition of Knopp's nutrient solution, which is the basis of modern nutrient solution composition.

In addition, the nutrient solution contains 0.2 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ ), 0.02 parts by weight of potassium monobasic phosphate (KH ₂ PO ₄ ), and an aqueous magnesium sulfate solution (MgSO ₄ ·7H _{2 )} based on 1 part by weight of the medium. O) It is preferable to mix 0.05 parts by weight.

The medium is best used at a concentration of 0.1% (w/w) of the agar per 1 L of the nutrient solution, followed by azinate, carboxymethyl cellulose (CMC), and superabsorbent polymer (SAP) in that order for adhesion and moisturizing. good night.

As shown in Figure 7, in the fifth step (S50), the submerged sporangia were mixed with nutrient solution and adhesive solution and sprayed on samples in the disaster area, and 16 weeks later, the live weight increased by 12.5 times.

The moss cultivation kit for forest restoration manufactured by the moss spore culture method of the present invention is maintained under the conditions below and can be performed as shown in FIG. 2.

① Shading: The moss cultivation kit for forest restoration sprayed with spores is placed in a greenhouse with 70% shading conditions of 20W LED.

② Irrigation: Use a spray nozzle to irrigate 6 times a day for 15 minutes each for 16 weeks.

③ Humidity: Maintain relative humidity at 60%.

④ Light intensity: Maintain 200 to 1,000 Lux for photosynthesis. (Effective photon bunch density (PPFD) 80 μmol/m²/s)

⑤ Temperature: Maintain between 20 and 25 ℃.

⑥ Hormone: Choose from abscisic acid (IBA), cytokinin (KT), gibberellin (GA), ethylene, and auxin. However, abscisic acid, cytokinin, and gibberellin are excellent at inducing moss germination and accelerate leaf growth, so abscisic acid and gibberellin are included.

⑦ pH: Adjust to 8~9.

⑧ Culture medium electrical conductivity: Maintain at 0.5~1.0 (mS/cm).

⑨ Fertilizer: When cultivating, excessively high concentration inhibits growth, so use Hyponex at 10% concentration, and the concentration is 0.5 ppm. It is desirable to spray the fertilizer once every two weeks in the summer and once a week in the winter.

⑩ Adhesion and moisturizing: 0.1% concentration Agar is selected first, followed by alginate, CMC, and super absorbent resin. As shown in Figure 7, after 16 weeks, live weight increased 12.5 times.

⑪ Upper LED lamp: Maintain 4.

⑫ Construction area: The higher the moss spore mixing ratio, the faster the growth rate. If it is less than 20%, covering the disaster area is slow, and if it is more than 40%, the increase in diesel efficiency is slow, so only 30% of the construction area is used.

Figure 4 is a photograph of samples taken from a disaster area for dispersing moss spores according to an embodiment of the present invention, and Figure 5 is a photograph taken 10 weeks after sampling of a disaster region.

Figure 6 is a photograph taken 10 weeks after spraying moss spores prepared according to the present invention to the disaster area sample of Figure 5, according to an embodiment of the present invention, and Figure 7 is a photograph of the disaster area sample prepared according to the present invention. This photo is taken 14 weeks after spraying the moss spores. When moss was identified 2 months after spraying, moss colonies and herbaceous plants appeared 4 to 5 months later, and woody plants appeared 7 months later.

By solving the above problem, the present invention can identify the pre-growth stage and spore generation stage of moss.

In addition, the present invention can restore forests by artificially cultivating moss spores, making them dormant, and spraying them on forests that have been lost when a disaster occurs.

As such, a person skilled in the art will understand that the technical configuration of the present invention described above can be implemented in other specific forms without changing the technical idea or essential features of the present invention.

Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive, and the scope of the present invention is indicated by the claims described later rather than the detailed description above, and the meaning and scope of the claims and their All changes or modified forms derived from the equivalent concept should be construed as falling within the scope of the present invention.

S10. The first step is to cut the sporangia into 10 to 30 mm pieces.
S20. A second step of drying the cut sporangia at 60 to 75° C. for 24 hours and then crushing the dried sporangia.
S30. The third step of immersing the crushed sporangia in the spore culture medium and then making it dormant.
S40. The fourth step of immersing the dormant sporangia in plant hormone solution for 24 hours.
S50. The fifth step of mixing and spraying the soaked sporangia with nutrient solution and adhesive solution.

Claims (5)

A first step of cutting the sporangia into 10 to 30 mm;
A second step of drying the cut sporangia at 60 to 75° C. for 24 hours and then crushing the dried sporangia;
A third step of immersing the crushed sporangia in a spore culture medium of pH 8 to 9 and then making them dormant;
A fourth step of immersing the dormant sporangia in a plant hormone solution for 24 hours;
A fifth step of mixing and spraying the soaked sporangia with nutrient solution and adhesive solution,
The sporangia are baby pine moss, rattail moss, or roof moss,
In the third step, the electrical conductivity of the spore culture is 0.5 to 1.0 mS/cm,
In the fourth step, the plant hormone solution is prepared by mixing equal amounts of abscisic acid, gibberellin, and auxin, and mixing 0.5 mg of the abscisic acid, gibberellin, and auxin mixture per 1 L of solvent to obtain 0.00005% (w/w). ,
In the fifth step, the nutrient solution is,
Prepared by mixing calcium nitrate (Ca(NO ₃ ) ₂ ), monobasic potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate aqueous solution (MgSO ₄ ·7H ₂ O) and medium,
Based on 1 part by weight of the medium, 0.2 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ ), 0.02 parts by weight of potassium monobasic phosphate (KH ₂ PO ₄ ), and 0.05 parts by weight of aqueous magnesium sulfate solution (MgSO ₄ ·7H ₂ O). Moss spore culture method characterized by mixing parts.
delete delete delete A moss spore culture kit for forest restoration, characterized in that it is cultured by the method of claim 1.