KR102488128B1 - 게놈의 큰 단편 직접 클로닝 및 dna 다중-분자 어셈블리를 위한 새로운 기술 - Google Patents

게놈의 큰 단편 직접 클로닝 및 dna 다중-분자 어셈블리를 위한 새로운 기술 Download PDF

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KR102488128B1
KR102488128B1 KR1020197031095A KR20197031095A KR102488128B1 KR 102488128 B1 KR102488128 B1 KR 102488128B1 KR 1020197031095 A KR1020197031095 A KR 1020197031095A KR 20197031095 A KR20197031095 A KR 20197031095A KR 102488128 B1 KR102488128 B1 KR 102488128B1
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exonuclease
dna
nucleic acid
acid molecules
target nucleic
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야우밍 장
하이롱 왕
준 푸
아드리안 프란시스 스뉴어트
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산동 유니버시티
테크니셰 유니베르시테트 드레스덴
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KR1020197031095A 2017-03-23 2017-08-02 게놈의 큰 단편 직접 클로닝 및 dna 다중-분자 어셈블리를 위한 새로운 기술 Active KR102488128B1 (ko)

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Application Number Priority Date Filing Date Title
CN201710177676.1A CN106995813B (zh) 2017-03-23 2017-03-23 基因组大片段直接克隆和dna多分子组装新技术
CN201710177676.1 2017-03-23
PCT/CN2017/000483 WO2018170614A1 (zh) 2017-03-23 2017-08-02 基因组大片段直接克隆和dna多分子组装新技术

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KR102488128B1 true KR102488128B1 (ko) 2023-01-12

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US (1) US20200010867A1 (enExample)
EP (1) EP3604524B1 (enExample)
JP (1) JP7106625B2 (enExample)
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CN (1) CN106995813B (enExample)
CA (1) CA3060585A1 (enExample)
DK (1) DK3604524T3 (enExample)
ES (1) ES2902210T3 (enExample)
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CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术
CN111684069A (zh) 2017-12-22 2020-09-18 G+Flas生命科学有限公司 嵌合基因组工程分子和方法
CN108676847A (zh) * 2017-12-30 2018-10-19 深圳人体密码基因科技有限公司 大片段基因组的捕获探针的制备方法及试剂盒
CN111088275B (zh) * 2018-10-23 2023-06-27 黄菁 Dna大片段的克隆方法
WO2020185584A1 (en) * 2019-03-08 2020-09-17 Zymergen Inc. Pooled genome editing in microbes
CN110846239B (zh) * 2019-11-29 2022-08-23 南京工业大学 具有高同源重组效率的重组解脂耶氏酵母菌及其构建方法和应用
CN112852849B (zh) * 2019-12-31 2023-03-14 湖北伯远合成生物科技有限公司 一种用于大片段dna无缝组装的系统及组装方法
IL294909A (en) * 2020-02-13 2022-09-01 Zymergen Inc Metagenomic library and natural product discovery platform
CN113355339B (zh) * 2020-03-05 2023-01-24 山东大学 一种大型基因簇的无痕定点改造方法及其应用
CN111440753A (zh) * 2020-03-16 2020-07-24 扬州大学 禽致病性大肠杆菌clbH基因缺失株及其构建方法和应用
CN116135982A (zh) * 2021-11-16 2023-05-19 北京光华生明生物科技有限公司 用于基因片段连接的应用方法和试剂盒
CN119072545A (zh) * 2022-03-23 2024-12-03 深圳华大生命科学研究院 表达元件组合、表达载体、宿主、应用及组装方法
CN115960733B (zh) * 2022-09-19 2023-11-24 苏州泓迅生物科技股份有限公司 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用
CN115948445A (zh) * 2022-12-12 2023-04-11 河南科技大学 谷氨酸棒杆菌CRISPR-Cpf1基因编辑系统、构建方法及应用
CN116355931A (zh) * 2023-05-29 2023-06-30 北京因诺惟康医药科技有限公司 一种快速分子克隆方法

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CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术

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DK3604524T3 (da) 2021-12-20
KR20190133200A (ko) 2019-12-02
EP3604524A4 (en) 2020-04-29
CN106995813B (zh) 2020-06-16
EP3604524A1 (en) 2020-02-05
JP2020519304A (ja) 2020-07-02
CA3060585A1 (en) 2018-09-27
WO2018170614A1 (zh) 2018-09-27
CN106995813A (zh) 2017-08-01
EP3604524B1 (en) 2021-10-06
ES2902210T3 (es) 2022-03-25
WO2018170614A8 (zh) 2018-11-01
US20200010867A1 (en) 2020-01-09

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