CN106995813B - 基因组大片段直接克隆和dna多分子组装新技术 - Google Patents

基因组大片段直接克隆和dna多分子组装新技术 Download PDF

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CN106995813B
CN106995813B CN201710177676.1A CN201710177676A CN106995813B CN 106995813 B CN106995813 B CN 106995813B CN 201710177676 A CN201710177676 A CN 201710177676A CN 106995813 B CN106995813 B CN 106995813B
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exonuclease
nucleic acid
acid molecule
length
dna
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CN106995813A (zh
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张友明
王海龙
符军
A.F.斯图尔特
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Shandong University
Technische Universitaet Dresden
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Shandong University
Technische Universitaet Dresden
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Priority to EP17901979.9A priority patent/EP3604524B1/en
Priority to DK17901979.9T priority patent/DK3604524T3/da
Priority to ES17901979T priority patent/ES2902210T3/es
Priority to JP2020500937A priority patent/JP7106625B2/ja
Priority to PCT/CN2017/000483 priority patent/WO2018170614A1/zh
Priority to KR1020197031095A priority patent/KR102488128B1/ko
Priority to CA3060585A priority patent/CA3060585A1/en
Priority to US16/578,385 priority patent/US20200010867A1/en
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CN201710177676.1A 2017-03-23 2017-03-23 基因组大片段直接克隆和dna多分子组装新技术 Active CN106995813B (zh)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CN201710177676.1A CN106995813B (zh) 2017-03-23 2017-03-23 基因组大片段直接克隆和dna多分子组装新技术
JP2020500937A JP7106625B2 (ja) 2017-03-23 2017-08-02 ゲノム大断片のダイレクトクローニングおよびｄｎａマルチ分子構築の新手法
DK17901979.9T DK3604524T3 (da) 2017-03-23 2017-08-02 Ny teknik til genomisk stor fragment direkte kloning og dna multi-molekylær sammensætning
ES17901979T ES2902210T3 (es) 2017-03-23 2017-08-02 Nueva técnica para la clonación directa de fragmentos genómicos grandes y el ensamblaje multimolecular de ADN
EP17901979.9A EP3604524B1 (en) 2017-03-23 2017-08-02 New technique for genomic large fragment direct cloning and dna multi-molecular assembly
PCT/CN2017/000483 WO2018170614A1 (zh) 2017-03-23 2017-08-02 基因组大片段直接克隆和dna多分子组装新技术
KR1020197031095A KR102488128B1 (ko) 2017-03-23 2017-08-02 게놈의 큰 단편 직접 클로닝 및 dna 다중-분자 어셈블리를 위한 새로운 기술
CA3060585A CA3060585A1 (en) 2017-03-23 2017-08-02 New technique for genomic large fragment direct cloning and dna multi-molecular assembly
US16/578,385 US20200010867A1 (en) 2017-03-23 2019-09-22 Method of homologous recombination of dna

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Families Citing this family (15)

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CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术
CN111684069A (zh) * 2017-12-22 2020-09-18 G+Flas生命科学有限公司 嵌合基因组工程分子和方法
CN108676847A (zh) * 2017-12-30 2018-10-19 深圳人体密码基因科技有限公司 大片段基因组的捕获探针的制备方法及试剂盒
CN111088275B (zh) * 2018-10-23 2023-06-27 黄菁 Dna大片段的克隆方法
JP2022524044A (ja) * 2019-03-08 2022-04-27 ザイマージェン インコーポレイテッド 微生物のプール型ゲノム編集
CN110846239B (zh) * 2019-11-29 2022-08-23 南京工业大学 具有高同源重组效率的重组解脂耶氏酵母菌及其构建方法和应用
CN112852849B (zh) * 2019-12-31 2023-03-14 湖北伯远合成生物科技有限公司 一种用于大片段dna无缝组装的系统及组装方法
CN115349128A (zh) * 2020-02-13 2022-11-15 齐默尔根公司 宏基因组文库和天然产物发现平台
CN113355339B (zh) * 2020-03-05 2023-01-24 山东大学 一种大型基因簇的无痕定点改造方法及其应用
CN111440753A (zh) * 2020-03-16 2020-07-24 扬州大学 禽致病性大肠杆菌clbH基因缺失株及其构建方法和应用
CN116135982A (zh) * 2021-11-16 2023-05-19 北京光华生明生物科技有限公司 用于基因片段连接的应用方法和试剂盒
WO2023178544A1 (zh) * 2022-03-23 2023-09-28 深圳华大生命科学研究院 表达元件组合、表达载体、宿主、应用及组装方法
CN115960733B (zh) * 2022-09-19 2023-11-24 苏州泓迅生物科技股份有限公司 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用
CN115948445A (zh) * 2022-12-12 2023-04-11 河南科技大学 谷氨酸棒杆菌CRISPR-Cpf1基因编辑系统、构建方法及应用
CN116355931A (zh) * 2023-05-29 2023-06-30 北京因诺惟康医药科技有限公司 一种快速分子克隆方法

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US20200010867A1 (en) 2020-01-09
WO2018170614A8 (zh) 2018-11-01
CA3060585A1 (en) 2018-09-27
EP3604524A4 (en) 2020-04-29
KR20190133200A (ko) 2019-12-02
KR102488128B1 (ko) 2023-01-12
EP3604524A1 (en) 2020-02-05
DK3604524T3 (da) 2021-12-20
WO2018170614A1 (zh) 2018-09-27
CN106995813A (zh) 2017-08-01
JP7106625B2 (ja) 2022-07-26
JP2020519304A (ja) 2020-07-02
ES2902210T3 (es) 2022-03-25

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