KR102415204B1 - 아프리카돼지열병 백신 조성물 - Google Patents

아프리카돼지열병 백신 조성물 Download PDF

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KR102415204B1
KR102415204B1 KR1020210048852A KR20210048852A KR102415204B1 KR 102415204 B1 KR102415204 B1 KR 102415204B1 KR 1020210048852 A KR1020210048852 A KR 1020210048852A KR 20210048852 A KR20210048852 A KR 20210048852A KR 102415204 B1 KR102415204 B1 KR 102415204B1
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김은진
엄태기
송경주
김민지
육근영
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(주)플럼라인생명과학
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Abstract

본 발명은 아프리카돼지열병에 대한 면역 반응 생성에 관여하는 폴리펩티드, 폴리뉴클레오티드, 플라스미드 및 이들을 함유하는 백신 조성물에 관한 것이다. 또한, 본 발명은 개체에서 아프리카돼지열병에 대한 면역 반응을 생성하는 방법에 관한 것이다. 또한, 본 발명은 아프리카돼지열병에 대한 면역 반응 생성에 관여하는 폴리펩티드, 폴리뉴클레오티드, 플라스미드 및 이들을 함유하는 아프리카돼지열병 치료 또는 예방용 약학 조성물에 관한 것이다. 또한, 본 발명은 개체에서 아프리카돼지열병을 예방 또는 치료하는 방법에 관한 것이다.

Description

아프리카돼지열병 백신 조성물{VACCINE COMPOSITION FOR AFRICAN SWINE FEVER}
본 발명은 아프리카돼지열병에 대한 면역 반응 생성에 관여하는 폴리펩티드, 폴리뉴클레오티드, 플라스미드 및 이들을 함유하는 백신 조성물에 관한 것이다. 또한, 본 발명은 개체에서 아프리카돼지열병에 대한 면역 반응을 생성하는 방법에 관한 것이다. 또한, 본 발명은 아프리카돼지열병에 대한 면역 반응 생성에 관여하는 폴리펩티드, 폴리뉴클레오티드, 플라스미드 및 이들을 함유하는 아프리카돼지열병 치료 또는 예방용 약학 조성물에 관한 것이다. 또한, 본 발명은 개체에서 아프리카돼지열병을 예방 또는 치료하는 방법에 관한 것이다.
아프리카돼지열병(African Swine Fever, ASF)은 치명적인 바이러스성 출혈성 돼지 전염병으로서, 일단 발병하게 되면 전파성이 매우 높으며 치료법이 없고 감염된 돼지들은 대부분 폐사하는 치사율이 매우 높기 때문에 양돈 산업에 엄청난 피해를 주는 질병이다. 따라서, 아프리카돼지열병이 발생하면 세계동물보건기구(OIE)에 발생 사실을 즉시 보고해야 하며, 돼지와 관련된 국제교역도 즉시 중단된다. 아프리카돼지열병은 세계동물보건기구(OIE)에서 매우 중요 질병으로 분류되어 있고, 우리나라에서는 가축전염병예방법상 제1종 법정전염병으로 지정하여 관리하고 있다.
아프리카돼지열병 바이러스(ASFV)는 아스파바이러스과(Asfarviridae) 아스피바이러스속(Asfivirus)에 속하는 약 200nm 정도의 DNA 바이러스이다. 아프리카돼지열병 바이러스는 사람이나 다른 동물에 감염되지 않고, 돼지과(Suidae)에 속하는 동물에만 감염되는 것으로 보고되고 있다.
감염된 동물로부터 나온 침, 호흡기 분비물, 오줌, 분변 등에는 아프리카돼지열병 바이러스가 대량 존재하며, 이러한 물질에 접촉한 정상 동물에게 아프리카돼지열병이 전파된다. 돼지가 죽은 후에도 혈액과 조직에 바이러스가 존속할 수 있기 때문에, 감염된 동물의 조직을 포함하고 있는 열처리하지 않은 잔반을 돼지에게 급여하면 급속하게 전파될 수 있다. 또는, 돼지들끼리 싸우는 동안 흘린 피, 혈액이 섞인 설사 등으로 인해 바이러스가 전파될 수도 있다. 또는, Ornithodoros spp.에 속하는 물렁 진드기(soft tick), 모기, 무는 파리 같은 흡혈 곤충 등이 아프리카돼지열병 바이러스를 보균하고 있다가 돼지를 물거나 흡혈할 때 질병을 전파하는 역할을 하는 매개체로 작용하기도 한다.
아프리카돼지열병은 모든 연령의 돼지가 감염될 수 있다. 이병률은 감염된 바이러스와 노출 경로에 따라 달라지며, 자연 감염 시 잠복기는 4일에서 19일까지 다양하다. 폐사율은 고병원성 바이러스에 감염된 경우 거의 100% 폐사된다.
아프리카돼지열병 바이러스는 병원성에 따라 고병원성, 중병원성 및 저병원성으로 분류될 수 있다. 고병원성 아프리카돼지열병 바이러스는 보통 심급성(감염 1-4일 후 돼지가 죽음) 및 급성형(감염 3-8일 후 돼지가 죽음) 질병을 일으키며, 중병원성 아프리카돼지열병 바이러스는 급성(감염 11-15일 후 돼지가 죽음) 및 아급성형(감염 20일 후 돼지가 죽음) 질병을 일으킨다. 저병원성 아프리카돼지열병 바이러스는 풍토병화된 지역에서만 보고되었으며 준임상형 또는 만성형 질병을 일으킨다.
아프리카돼지열병은 발생 초기에, 특히 이환된 돼지의 수가 적을 때에는 질환의 진단이 쉽지 않다. 그 이유는 아프리카돼지열병의 임상 증상은 폐혈증성 살모넬라증(Septicaemic salmonellosis), 돼지피부염신증증후군(porcine dermatitis nephropathy syndrome, PDNS) 등 다른 출혈성 돼지질병과 혼동되기 때문이다. 혈액 및 내부 장기에서 바이러스를 검출하는 방법, 감염돈의 혈청에서 항체를 검출하는 방법 등 실험실적 진단을 통해서만 정확한 구별이 가능하며, 아프리카돼지열병이라고 진단되는 경우에는 이미 질병이 널리 전염되어 있는 경우가 많다. 예를 들어, 중국에서는 2018년에 아프리카돼지열병이 최초로 확인된 이후 9개월이 채 안 되는 기간에 중국 전 행정구역에서 아프리카돼지열병이 발생할 정도로 아프리카돼지열병의 전염력은 매우 높다. 또한, 세계 최대 양돈국가인 중국에서 아프리카돼지열병으로 인해 2019년에 약 1억 3천만 마리의 돼지가 폐사되거나 살처분되었으며, 이는 아프리카돼지열병 발생 전 4억 3천 마리의 돼지에서 약 1/3 가량이 감소되었음을 의미한다.
아프리카돼지열병 백신의 연구는 1960년대 이후 꾸준히 진행되고 있다. 약독화 생백신, 불활화 백신, 재조합 단백질 백신, 바이러스 벡터 백신 등 현재까지 다양한 시도가 이루어졌으나, 상업적으로 적용될 수 있을 만큼 효과가 우수한 백신은 개발되지 못하고 있는 실정이다. 최근 불활화 백신(inactivated vaccine)을 접종하여 중화항체가 형성되어도 실제 공격접종 (challenge) 시에는 방어효과가 없거나 미미하였다는 연구 결과가 발표된바 있으며, 이를 통해 중화항체를 생성하는 것 이외에 세포성 면역 반응을 유도하는 것이 백신의 바이러스에 대한 방어능을 향상시키는데 중요한 메커니즘임을 알 수 있다(Takamatsu et al., Virus Res. 2013 Apr, 173(1):110-21.).
또한, 돼지에 치명적인 아프리카돼지열병에 효과적인 백신을 개발하기 어려운 이유 중 하나는 크고 복잡한 아프리카돼지열병 바이러스의 특성 때문이다. 일반적인 바이러스가 10~12개의 단백질을 가지고 있다면, 아프리카돼지열병 바이러스는 150개 이상의 단백질을 가지고 있고 바이러스 종류(24가지 유전형)도 다양하다. 따라서, 전문가들은 향후 최소한 10년 이내에는 백신의 상용화가 어려울 것이라고 전망해왔다.
따라서, 아프리카돼지열병에 대한 백신 또는 치료제가 절실하게 요구된다. 특히, 아프리카돼지열병은 안정적이고 효과적으로 미리 예방하는 것이 중요하며, 이를 위한 신규 백신의 미충족 수요가 매우 크다.
본 발명의 일 목적은 아프리카돼지열병에 대한 면역 반응을 생성할 수 있는 폴리펩티드 및 이를 코딩하는 폴리뉴클레오티드를 제공하는 것이다.
본 발명의 다른 목적은 아프리카돼지열병에 대한 면역 반응을 생성할 수 있는 플라스미드를 제공하는 것이다.
본 발명의 또 다른 목적은 아프리카돼지열병에 대한 백신 조성물을 제공하는 것이다.
본 발명의 또 다른 목적은 개체에서 아프리카돼지열병에 대한 면역 반응을 생성하는 방법을 제공하는 것이다.
본 발명의 또 다른 목적은 개체에서 아프리카돼지열병 치료 또는 예방용 약학 조성물을 제공하는 것이다.
본 발명의 또 다른 목적은 개체에서 아프리카돼지열병을 치료 또는 예방하는 방법을 제공하는 것이다.
상기 목적을 달성하기 위해, 본 발명의 일 양태는 아프리카돼지열병 바이러스의 유전자 p30, p54, C-type lectin, CD2v, p49, pp62, EP364R, F317L, A104R 및 K205R 중 하나 이상의 아미노산 서열을 포함하는 폴리펩티드를 제공한다.
본 발명의 또 다른 일 양태는 서열번호 1 내지 10의 아미노산 서열 또는 이와 90% 이상의 상동성을 갖는 아미노산 서열 중 하나 이상을 포함하는 폴리펩티드를 제공한다.
본 명세서에서 사용되는 용어 "상동성"은 주어진 폴리펩티드 서열 또는 폴리뉴클레오티드 서열과 일치하는 정도를 의미하며 백분율로 표시될 수 있다. 본 명세서에서, 주어진 폴리펩티드 서열과 동일하거나 유사한 활성을 갖는 그의 상동성 서열이 "% 상동성"으로 표시된다. 상기 상동성은 예를 들면, 점수(score), 동일성(identity) 및 유사도(similarity) 등의 매개 변수(parameter)들을 계산하는 표준 소프트웨어, 예를 들어 BLAST 2.0을 이용하거나, 정의된 엄격한 조건하에서 썼던 혼성화 실험에 의해 서열을 비교함으로써 확인할 수 있으며, 정의되는 적절한 혼성화 조건은 당업자에게 잘 알려진 방법으로 결정될 수 있다.
서열번호 1 내지 10의 아미노산 서열은 순서대로 각각, 아프리카돼지열병 바이러스의 다양한 균주들로부터 유전자 p30 (CP204L), p54 (E183L), C-type lectin (EP153R), CD2v (EP402R), p49 (B438L), pp62 (CP530R), EP364R, F317L, A104R, K205R의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다.
상기 서열번호 1 내지 10의 아미노산 서열 또는 이와 90% 이상의 상동성을 갖는 아미노산 서열 중 하나 이상을 포함하는 폴리펩티드는 아프리카돼지열병에 대한 면역 반응을 생성할 수 있는 항원으로서 효과적으로 작용할 수 있다.
상기 폴리펩티드는 상기 아미노산 서열 중 하나를 포함할 수도 있고, 둘 이상을 포함할 수도 있다. 또한, 상기 폴리펩티드는 Kozak 서열, IgE leader 서열, 유비퀴틴(ubiquitin) 서열 및/또는 절단 부위(cleavage site) 서열을 더 포함할 수 있다. 예를 들면, 상기 폴리펩티드는 Kozak 서열 - IgE leader 서열 - 서열번호 1의 아미노산 서열로 구성될 수 있다. 또다른 예로서, 상기 폴리펩티드는 Kozak 서열 - IgE leader 서열 - 유비퀴틴(ubiquitin) 서열 - 서열번호 1의 아미노산 서열로 구성될 수 있다. 또다른 예로서, 상기 폴리펩티드는 Kozak 서열 - IgE leader 서열 - 유비퀴틴(ubiquitin) 서열 - 서열번호 1의 아미노산 서열 - 절단 부위(cleavage site) 서열 - 서열번호 2의 아미노산 서열로 구성될 수 있다. 상기 유비퀴틴 서열은 바람직하게는 서열번호 41일 수 있고, 상기 절단 부위 서열은 바람직하게는 서열번호 43일 수 있다.
본 발명의 일 양태는 아프리카돼지열병 바이러스의 유전자 p30, p54, C-type lectin, CD2v, p49, pp62, EP364R, F317L, A104R 및 K205R 중 하나 이상의 염기 서열을 포함하는 폴리뉴클레오티드를 제공한다.
본 발명의 또 다른 일 양태는 서열번호 11 내지 20의 염기 서열 또는 이와 90% 이상의 상동성을 갖는 염기 서열 중 하나 이상을 포함하는 폴리뉴클레오티드를 제공한다.
또는, 본 발명의 또 다른 양태는 서열번호 1 내지 10의 아미노산 서열 또는 이와 90% 이상의 상동성을 갖는 아미노산 서열 중 하나 이상을 코딩하는 폴리뉴클레오티드를 제공한다. 상기 서열번호 1 내지 10의 아미노산 서열을 코딩하는 폴리뉴클레오티드는 각각 서열번호 11 내지 20의 염기 서열일 수 있다.
서열번호 11 내지 20의 염기 서열은 순서대로 각각, 아프리카돼지열병 바이러스의 다양한 균주들로부터 유전자 p30 (CP204L), p54 (E183L), C-type lectin (EP153R), CD2v (EP402R), p49 (B438L), pp62 (CP530R), EP364R, F317L, A104R, K205R의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다.
또 다르게는, 상기 폴리뉴클레오티드는 서열번호 1 내지 10의 아미노산 서열 또는 서열번호 11 내지 20의 염기 서열이 면역 반응을 생성하고자 하는 대상체에게 최적화된 코돈으로 변경된 것일 수 있다. 상기 대상체는 예를 들어 동물이다. 상기 동물은 예를 들어 돼지, 소, 말, 양, 염소, 사슴, 인간 등을 포함하는 포유류 동물일 수 있다. 바람직하게는, 상기 동물은 돼지이다.
상기 폴리뉴클레오티드는 상기 염기 서열 중 하나를 포함할 수도 있고, 둘 이상을 포함할 수도 있다. 또한, 상기 폴리뉴클레오티드는 Kozak 서열, IgE leader 서열, 유비퀴틴(ubiquitin) 서열 및/또는 절단 부위(cleavage site) 서열을 더 포함할 수 있다. 예를 들면, 상기 폴리뉴클레오티드는 Kozak 서열 - IgE leader 서열 - 서열번호 11의 염기 서열로 구성될 수 있다. 또다른 예로서, 상기 폴리뉴클레오티드는 Kozak 서열 - IgE leader 서열 - 유비퀴틴(ubiquitin) 서열 - 서열번호 11의 염기 서열로 구성될 수 있다. 또다른 예로서, 상기 폴리뉴클레오티드는 Kozak 서열 - IgE leader 서열 - 유비퀴틴(ubiquitin) 서열 - 서열번호 11의 염기 서열 - 절단 부위(cleavage site) 서열 - 서열번호 12의 염기 서열로 구성될 수 있다. 상기 유비퀴틴 서열은 바람직하게는 서열번호 42일 수 있고, 상기 절단 부위 서열은 바람직하게는 서열번호 44일 수 있다.
본 발명의 일 양태는 아프리카돼지열병 바이러스의 유전자 p30, p54, C-type lectin, CD2v, p49, pp62, EP364R, F317L, A104R 및 K205R 중 하나 이상의 염기 서열을 포함하는 폴리뉴클레오티드를 포함하는 플라스미드를 제공한다.
본 발명의 또 다른 일 양태는 서열번호 11 내지 20의 염기 서열 또는 이와 90% 이상의 상동성을 갖는 염기 서열 중 하나 이상을 포함하는 폴리뉴클레오티드를 포함하는 플라스미드를 제공한다.
본 발명의 또 다른 양태는 서열번호 1의 아미노산 서열 또는 서열번호 1의 서열과 90%의 상동성을 갖는 아미노산 서열 중 하나 이상을 코딩하는 폴리뉴클레오티드를 포함하는 플라스미드를 제공한다.
본 명세서에서 사용되는 용어 "플라스미드"는 적합한 숙주 또는 대상체 내에서 DNA를 발현시킬 수 있는 적합한 조절 서열에 작동가능하게 연결된 DNA 서열을 함유하는 DNA 제조물을 의미한다. 상기 플라스미드는 벡터, 파지 입자, 또는 간단하게 잠재적 게놈 삽입물일 수 있다. 본 명세서에서 플라스미드는 벡터(또는 바이러스 벡터)와 상호 교환적으로 사용될 수 있다.
상기 플라스미드는 당업계에 알려져 있는 통상의 방법에 따라 제조될 수 있다. 예를 들어, 유전자 클로닝을 통해 상기 폴리뉴클레오티드를 플라스미드 벡터에 삽입하여, 본 발명에 따른 플라스미드를 제조하여 DNA 백신으로 사용할 수 있다.
상기 플라스미드는 상기 폴리뉴클레오티드 중 하나를 포함할 수도 있고, 둘 이상을 포함할 수도 있다. 또한, 상기 폴리뉴클레오티드는 Kozak 서열, IgE leader 서열, 유비퀴틴(ubiquitin) 서열 및/또는 절단 부위(cleavage site) 서열을 더 포함할 수 있다.
예를 들면, 상기 플라스미드는 서열번호 31의 염기 서열을 포함할 수 있으며, 여기서 서열번호 31은 서열번호 11 내지 20의 염기 서열이 순차적으로 연결된 것이다. 또다른 예로서, 상기 플라스미드는 서열번호 36의 염기 서열을 포함할 수 있으며, 여기서 서열번호 36은 유비퀴틴(ubiquitin) 서열과 서열번호 11 내지 20의 염기 서열이 순차적으로 연결된 것이다. 또다른 예를 들면, 상기 플라스미드는 서열번호 50의 염기 서열을 포함할 수 있으며, 여기서 서열번호 50은 유비퀴틴(ubiquitin) 서열과 서열번호 11 내지 20의 염기 서열이 순차적으로 연결된 것으로서, 상기 서열번호 11 내지 20의 염기 서열 각각은 절단 부위(cleavage site) 서열로 순차적으로 연결된 것이다.
상기 유비퀴틴 서열은 바람직하게는 서열번호 42의 염기 서열일 수 있다. CD8+ T 세포는 사이토졸(cytosol)에 위치한 종양 유전자 생성물 또는 바이러스 항원과 같은 내인성 항원(endogenous antigen)으로부터 유도된 MHC 클래스 I 관련 펩타이드(MHC class I-associated peptides)를 인식한다. 이러한 MHC 클래스 I 분자가 항원 제시(antigen presentation)하기 전에, 항원은 유비퀴틴화(ubiquitionation)되고 프로테아좀(proteasome)에 의해 항원성 펩티드로 처리된다. 따라서, 유비퀴틴 융합 단백질(ubiquitin-fused proteins)은 프로테아좀 의존성 분해 경로(proteasome dependent degradation pathway)로 들어가서 MHC 클래스 I 펩티드 제시 능력을 향상시켜, 세포성 면역반응(CTL, Cytotoxic T Lymphocyte response)의 유도를 증가시킬 수 있다.
상기 절단 부위 서열은 바람직하게는 서열번호 44의 염기 서열일 수 있다. 상기 절단 부위(cleavage site)는 프로테아제(protease)에 의해 인식되어 절단되는 펩타이드를 의미한다. 본 발명에서는 상기 폴리뉴클레오티드를 포함하는 플라스미드로부터 발현된 폴리펩티드가 분해효소에 의해 절단될 수 있도록 하며, 이를 통해 절단된 각각의 폴리펩티드가 아프리카돼지열병에 대한 면역 반응을 생성할 수 있는 항원으로서 효과적으로 작용할 수 있게 한다. 상기 절단 부위는 세포 내 존재하는 내인성 효소에 의해 절단될 수 있고, 구체적으로 퓨린 프로테아제(furine protease)에 의해 절단될 수 있으나, 이에 제한되는 것은 아니다.
또다른 예로서, 상기 플라스미드는 서열번호 32의 염기 서열을 포함할 수 있으며, 여기서 서열번호 32는 서열번호 11 내지 14의 염기 서열이 순차적으로 연결된 것이다. 또는, 상기 플라스미드는 서열번호 37의 염기 서열을 포함할 수 있으며, 여기서 서열번호 37은 유비퀴틴(ubiquitin) 서열과 서열번호 11 내지 14의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 33의 염기 서열을 포함할 수 있으며, 여기서 서열번호 33은 서열번호 15 내지 20의 염기 서열이 순차적으로 연결된 것이다. 또는, 상기 플라스미드는 서열번호 38의 염기 서열을 포함할 수 있으며, 여기서 서열번호 38은 유비퀴틴(ubiquitin) 서열과 서열번호 15 내지 20의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 34의 염기 서열을 포함할 수 있으며, 여기서 서열번호 34는 서열번호 11, 12, 16, 13 및 14의 염기 서열이 순차적으로 연결된 것이다. 또는, 상기 플라스미드는 서열번호 39의 염기 서열을 포함할 수 있으며, 여기서 서열번호 39는 유비퀴틴(ubiquitin) 서열과 서열번호 11, 12, 16, 13 및 14의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 35의 염기 서열을 포함할 수 있으며, 여기서 서열번호 35는 서열번호 15, 17, 18, 19 및 20의 염기 서열이 순차적으로 연결된 것이다. 또는, 상기 플라스미드는 서열번호 40의 염기 서열을 포함할 수 있으며, 여기서 서열번호 40은 유비퀴틴(ubiquitin) 서열과 서열번호 15, 17, 18, 19 및 20의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 50의 염기 서열을 포함할 수 있으며, 여기서 서열번호 50은 서열번호 42의 유비퀴틴(ubiquitin) 서열; 및 서열번호 44의 절단 부위 서열을 포함하는, 서열번호 11, 44, 12, 44, 13, 44, 14, 44, 15, 44, 16, 44, 17, 44, 18, 44, 19, 44, 20의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 51의 염기 서열을 포함할 수 있으며, 여기서 서열번호 51은 서열번호 42의 유비퀴틴(ubiquitin) 서열; 및 서열번호 44의 절단 부위 서열을 포함하는, 서열번호 11, 44, 12, 44, 13, 44, 14의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 52의 염기 서열을 포함할 수 있으며, 여기서 서열번호 52는 서열번호 42의 유비퀴틴(ubiquitin) 서열; 및 서열번호 44의 절단 부위 서열을 포함하는, 서열번호 15, 44, 16, 44, 17, 44, 18, 44, 19, 44, 20의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 53의 염기 서열을 포함할 수 있으며, 여기서 서열번호 53은 서열번호 42의 유비퀴틴(ubiquitin) 서열; 및 서열번호 44의 절단 부위 서열을 포함하는, 서열번호 11, 44, 12, 44, 16, 44, 13, 44, 14의 염기 서열이 순차적으로 연결된 것이다.
또다른 예로서, 상기 플라스미드는 서열번호 54의 염기 서열을 포함할 수 있으며, 여기서 서열번호 54는 서열번호 42의 유비퀴틴(ubiquitin) 서열; 및 서열번호 44의 절단 부위 서열을 포함하는, 서열번호 15, 44, 17, 44, 18, 44, 19, 44, 20의 염기 서열이 순차적으로 연결된 것이다.
본 발명의 또 다른 양태는 본 발명의 폴리펩티드, 본 발명의 폴리뉴클레오티드, 또는 본 발명의 플라스미드를 포함하는 아프리카돼지열병 백신 조성물을 제공한다.
상기 아프리카돼지열병 백신 조성물에서 상기 폴리펩티드, 상기 폴리뉴클레오티드, 상기 플라스미드는 달리 언급하지 않는 한 상기한 바와 같다.
용어 "백신"은 개체에 면역을 주는 항원을 함유한 생물학적인 제제로서, 질환 예방을 위하여 사람이나 동물에게 투여, 예컨대 주사하거나 경구 투여함으로써 면역이 생기게 하는 면역원성 또는 항원성 물질을 말한다.
상기 백신은 DNA 백신일 수 있다. 용어 "DNA 백신"이라 함은, 병원균이나 바이러스 등의 유전자들 중 일부를 인공적으로 복제한 후 이를 투여함으로써 면역 반응을 야기하는 백신을 의미한다. 이러한 DNA 백신은 기존 단백질 백신에 비해 다양한 장점을 갖는다: i) 순수한 표적 병원체 항원의 유전정보만으로 합성제작이 가능하므로 위험한 병원체를 직접 취급할 필요가 없으며, ii) 독성 유발에 필요한 유전자들 중 일부만을 사용하므로 투여체에 투여되더라도 별다른 독성을 나타낼 염려가 없고, iii) 플라스미드 DNA만으로 구성되는 단순함 때문에 갑자기 발생하는 다양한 감염병에 대해 신속하게 대응하여 백신을 개발하는 것이 가능하다.
상기 아프리카돼지열병 백신 조성물은 바람직하게는 아프리카돼지열병 바이러스에 대한 방어 면역능을 갖는 것일 수 있다.
본 발명의 백신 조성물은 수의학적으로 허용 가능한 담체를 포함할 수 있다. 용어, "수의학적으로 허용 가능한 담체"란 임의의 및 모든 용매, 분산 매질, 코팅제, 항원보강제, 안정제, 희석제, 보존제, 항균제 및 항진균제, 등장성 작용제, 흡착지연제 등을 포함한다. 백신 조성물에 포함될 수 있는 담체, 부형제, 희석제로는 락토즈, 덱스트로스, 슈크로스, 솔비톨, 만니톨, 자일리톨, 말티톨, 전분, 글리세린, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘포스페이트, 칼슘실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유를 들 수 있다. 또한, 본 발명의 백신용 조성물은 각각 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. 제제화할 경우에는 보통 사용되는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제할 수 있다. 바람직하게는, Poly-L-Glutamic acid를 사용하여 안정화에 도움을 줄 수 있다.
상기 백신 조성물은 다양한 형태로 개체에 주입될 수 있다. "주입"은 피하주사, 근육내 주사, 피하내 주사, 복막내 주사, 비강투여, 구강투여, 경피투여 및 경구투여로 구성된 군에서 선택된 어느 하나의 방법으로 수행될 수 있다.
상기 백신 조성물은 면역 반응을 개선 또는 강화시키기 위하여 하나 이상의 보조제 등을 포함할 수 있다. 적절한 보조제에는 펩티드, 알루미늄 하이드록시드, 알루미늄 포스페이트, 알루미늄 옥시드 및 Marcol 52 같은 미네랄 오일 또는 식물성 오일 및 하나 이상의 유화제로 구성된 조성물 또는 리졸세시틴, 다가 양이온, 다가 음이온 같은 표면 활성물질 등이 포함된다.
본 발명의 일 양태는 본 발명의 백신 조성물을 개체에게 투여하는 단계를 포함하는, 개체에서 아프리카돼지열병 바이러스에 대한 면역 반응을 생성하는 방법을 제공한다.
상기 면역 반응 생성 방법에서, 상기 백신 조성물은 달리 언급하지 않는 한 상기한 바와 같다.
본 명세서에서 사용되는 용어 "면역 반응"은 항원의 도입에 대한 반응으로의 숙주의 면역계의 활성화를 의미한다. 면역 반응은 세포 반응 또는 체액 반응, 또는 둘 모두의 형태일 수 있다.
본 명세서에서 사용되는 용어 "개체"는 인간을 포함한 동물을 의미하며, 예를 들어 포유류, 보다 구체적으로 돼지, 소, 말, 양, 염소, 사슴, 인간 등일 수 있다. 바람직하게는, 돼지이다. 상기 용어 "개체"는 대상체 또는 숙주와 상호교환적으로 사용될 수 있다.
상기 면역 반응 생성 방법에서 본 발명의 백신 조성물은 효과량의 유효 성분, 즉, 전체 재조합 폴리펩티드, 재조합 폴리뉴클레오티드 또는 이를 포함하는 플라스미드를 약학적으로 허용가능한 담체 및 보조제와 함께 포함할 수 있다. 용어 "효과량"은 백신의 성분이 백신접종되는 동물에서 아프리카돼지열병 바이러스에 대해 특이적 면역 반응을 유도하기에 충분한 양임을 의미한다. 효과량은 당 분야의 숙련자에 의해 쉽게 결정될 수 있고, 예를 들면 동물에서의 통상적인 실험을 통해 결정될 수 있다.
상기 투여는 DNA 백신의 투여에 적합한 임의의 경로로 투여될 수 있고, 예를 들면 피하, 근육내, 복강내 또는 정맥내 주사에 의해 투여될 수 있다.
본 발명의 또다른 일 양태는 본 발명의 백신 조성물, 폴리펩티드, 폴리뉴클레오티드 또는 플라스미드를 개체에게 투여하는 단계를 포함하는, 개체에서 아프리카돼지열병을 치료 또는 예방하는 방법을 제공한다.
본 발명의 또다른 일 양태는 본 발명에 따른 폴리펩티드, 폴리뉴클레오티드 또는 플라스미드를 포함하는 아프리카돼지열병 치료 또는 예방용 약학 조성물을 제공한다.
본 발명에 따른 아프리카돼지열병 치료 또는 예방 방법과 아프리카돼지열병 치료 또는 예방용 약학 조성물에서, 각 용어들은 특별히 언급하지 않는 한 상기한 바와 동일한 의미를 갖는다.
본 발명에서 사용되는 용어 "예방"은 본 발명에 따른 조성물의 투여에 의해 아프리카돼지열병이 억제 또는 지연되는 모든 행위를 의미한다. 또한, 본 발명에서 사용되는 용어 "치료"는 본 발명에 따른 조성물의 투여에 의해 아프리카돼지열병의 증상 호전, 완치, 개선, 또는 일부 치료되는 모든 행위를 의미한다.
본 발명에 따른 백신 조성물은 DNA 백신으로서, 아프리카돼지열병 바이러스에 대해 우수한 방어 면역능을 갖는다. 특히, 본 발명에 따른 백신 조성물은 아프리카돼지열병에 대해 세포성 면역반응을 유도하는 효과가 매우 우수하다.
본 발명의 백신 조성물을 투여하는 경우, 접종된 개체에서 아프리카돼지열병에 대한 항체가 현저하게 높은 수준으로 생산되며, 이에 따라 면역 반응이 효과적으로 나타나고 아프리카돼지열병에 대해 우수한 예방 효과를 갖는다.
도 1은 아프리카돼지열병 (African Swine Fever, ASF) 바이러스 유전자를 포함하는 벡터의 개열 지도이며, Kozak 서열, IgE leader 서열, 유비퀴틴(ubiquitin) 서열 및 퓨린 절단 부위(Furin cleavage site) 서열을 포함하고, ASF 바이러스 유전자로서 p30, p54, C-type lectin, CD2v, p49, pp62, EP364R, F317L, A104R 및 K205R를 포함하는 벡터에 관한 것이다.
도 2는 ASF 바이러스 유전자를 포함하는 벡터의 개열 지도이며, Kozak 서열, IgE leader 서열, 유비퀴틴 서열 및 퓨린 절단 부위 서열을 포함하고, ASF 바이러스 유전자로서 p30, p54, C-type lectin 및 CD2v를 포함하는 벡터에 관한 것이다.
도 3은 ASF 바이러스 유전자를 포함하는 벡터의 개열 지도이며, Kozak 서열, IgE leader 서열, 유비퀴틴 서열 및 퓨린 절단 부위 서열을 포함하고, ASF 바이러스 유전자로서 p49, pp62, EP364R, F317L, A104R 및 K205R를 포함하는 벡터에 관한 것이다.
도 4는 ASF 바이러스 유전자를 포함하는 벡터의 개열 지도이며, Kozak 서열, IgE leader 서열, 유비퀴틴 서열 및 퓨린 절단 부위 서열을 포함하고, ASF 바이러스 유전자로서 p30, p54, pp62, C-type lectin 및 CD2v를 포함하는 벡터에 관한 것이다.
도 5는 ASF 바이러스 유전자를 포함하는 벡터의 개열 지도이며, Kozak 서열, IgE leader 서열, 유비퀴틴 서열 및 퓨린 절단 부위 서열을 포함하고, ASF 바이러스 유전자로서 p49, EP364R, F317L, A104R 및 K205R를 포함하는 벡터에 관한 것이다.
도 6은 ASF 바이러스 유전자를 포함하는 DNA 백신 접종 후, ASF 바이러스 항원(p30, p54 또는 CD2v)에 의해 생성된 세포독성 T 세포(CD8+ T 세포)의 수를 보여주는 그래프로서, 상기 DNA 백신에 의해 유도된 세포성 면역반응의 수준을 나타낸다. 'Mock'은 ASF 바이러스 유전자가 포함되지 않은 DNA 백신이 투여된 대조군, 'ASF_4G'는 ASF 바이러스 유전자(p30, p54, C type lectin 및 CD2v)를 포함하며 유비퀴틴을 포함하지 않는 DNA 백신이 투여된 실험군 1, 'ASF_Ubi_4G'는 ASF 바이러스 유전자(p30, p54, C type lectin 및 CD2v)를 포함하며 유비퀴틴을 포함하는 DNA 백신이 투여된 실험군 2를 의미한다.
도 7은 ASF 바이러스 유전자를 포함하는 DNA 백신 접종 후, ASF 바이러스에 감염된 돼지의 생존율을 보여주는 그래프이다. 'Mock'은 ASF 바이러스 유전자가 포함되지 않은 DNA 백신이 투여된 대조군, 'ASF_Ubi_10G'는 ASF 바이러스 유전자(p30, p54, C-type lectin, CD2v, p49, pp62, EP364R, F317L, A104R 및 K205R)를 포함하며 유비퀴틴을 포함하는 DNA 백신이 투여된 실험군을 의미한다.
이하 본 발명을 실시예에 의해 보다 상세하게 설명한다. 그러나 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것은 아니다.
실시예 1. 아프리카돼지열병 바이러스 유전자
서열번호 1의 아미노산 서열 및 서열번호 11의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 p30 (CP204L) 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 1의 아미노산 서열 및 서열번호 11의 염기 서열은 아프리카돼지열병 바이러스 균주 ANG/70 (GenBank accession no. EU874271), Malawi/1978 (GenBank accession no. JQ744998), NAM/1/80 (GenBank accession no. JQ745005), Dedza (GenBank accession no. JQ745028), BUR/90/1 (GenBank accession no. EU874299), MOZ/94/1 (GenBank accession no. EU874263), DED 91/1 (GenBank accession no. KC867513), GUL 88/1 (GenBank accession no. KC867514), KAC 91/2 (GenBank accession no. KF736439), KANA 89/1 (GenBank accession no. KF736440), Killean I (GenBank accession no. JQ764860), Killean II (GenBank accession no. KC867521), Killean III (GenBank accession no. JQ764861), Kimakia I (GenBank accession no. JQ764954), Kimakia II (GenBank accession no. KC867515), KIRT 89/2 (GenBank accession no. JQ764858), KIRT 89/3 (GenBank accession no. JQ764856), KIRT 89/4 (GenBank accession no. JQ764857), KLI 88/2 (GenBank accession no. KC867516), LIL 89/1 (GenBank accession no. KC867517), LIL 90/1 (GenBank accession no. KF736437), LIV 5/40 (GenBank accession no. KC867518), LIV 9/31 (GenBank accession no. JQ764966), LIV 9/35 (GenBank accession no. JQ764965), LIV 10/11 (GenBank accession no. KC867519), LIV 12/17 (GenBank accession no. JQ764967), Mchinji 075 (GenBank accession no. JQ764880), MOZ 2001/1 (GenBank accession no. KC867524), MPO 89/1 (GenBank accession no. KC867520), NYA1/2 (GenBank accession no. EU874302), SAL 92/1 (GenBank accession no. KF736441), TEN 89/1 (GenBank accession no. KF736442), THY 90/1 (GenBank accession no. KF736438), Trench (GenBank accession no. JQ764859), MOZ/1960 (GenBank accession no. EU874309), Lillie (GenBank accession no. EU874306), 24823 (GenBank accession no. KC867500), MOZ/1979 (GenBank accession no. EU874310), SPEC/154 (GenBank accession no. EU874291), SPEC/205 (GenBank accession no. EU874305), SPEC/209 (GenBank accession no. EU874290), SPEC/257 (GenBank accession no. EU874265), MOZ/94/8 (GenBank accession no. EU874276), E70 (GenBank accession no. AF462272), ZAM/2017/Mbala/1 (GenBank accession no. LC322014), CN201801 (GenBank accession no. MH735141), DB/HLJ/2018 (GenBank accession no. MK333184), M-78 (GenBank accession no. MK211505), BA71 (GenBank accession no. KP055815), BA71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken06.Bus (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/R25/2015 (GenBank accession no. MH025918), Uganda/R8/2015 (GenBank accession no. MH025916), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_03029_C201 (GenBank accession no. MG939587), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894), Ken05/Tk1 (GenBank accession no. KM111294), Ken05/Tk6 (GenBank accession no. HM745363), Ken05.DPk2 (GenBank accession no. HM745368), Ken08WH/4 (GenBank accession no. HM745390), Ken08Tk.2/1 (GenBank accession no. HM745380), Ken09Tk.13/1 (GenBank accession no. HM745382), Ken09Tk.19/2 (GenBank accession no. HM745386), Ken09Tk.19/11 (GenBank accession no. HM745388)로부터 p30 (CP204L) 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 2의 아미노산 서열 및 서열번호 12의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 p54 (E183L) 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 2의 아미노산 서열 및 서열번호 12의 염기 서열은 아프리카돼지열병 바이러스 균주 Tengani/60 (GenBank accession no. KF015886), ANG/70 (GenBank accession no. EU874327), BA71 (GenBank accession no. KP055815), Malawi/1978 (GenBank accession no. KC662380), Brazil/79 (GenBank accession no. KC535549), DomRep/79 (GenBank accession no. FJ238534), KAV/89/1 (GenBank accession no. KF015902), BUR/90/1 (GenBank accession no. EU874363), MOZ/94/1 (GenBank accession no. EU874342), CHK 89/2 (GenBank accession no. KF015921), KAC 91/2 (GenBank accession no. KF736421), KANA 89/1 (GenBank accession no. KF736422), Killean III (GenBank accession no. KF736423), Kimakia I (GenBank accession no. KF015924), KIRT 89/4 (GenBank accession no. KF736414), LIL 90/1 (GenBank accession no. KF736416), LIV 9/31 (GenBank accession no. KF015928), MAN 89/2 (GenBank accession no. KF015940), MOZ 2001/1 (GenBank accession no. KF736428), MPO 89/1 (GenBank accession no. KF736418), Trench (GenBank accession no. KF736420), MOZ/1960 (GenBank accession no. EU874371), Lillie (GenBank accession no. EU874341), Madagascar (GenBank accession no. KC662387), ZIM/92/1 (GenBank accession no. EU874345), SPEC/205 (GenBank accession no. EU874329), Co62 (GenBank accession no. FJ174387), E70 (GenBank accession no. FJ174389), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), Hu90 (GenBank accession no. FJ174399), SS81 (GenBank accession no. FJ174403), Ori90 (GenBank accession no. FJ174407), Nu98.8B (GenBank accession no. FJ174418), OURT 88/3 (GenBank accession no. AM712240), Almodovar 99 (GenBank accession no. DQ028315), Almodovar 99/NE1 (GenBank accession no. DQ028317), Georgia 2007 (GenBank accession no. FR682468), Angola (GenBank accession no. FJ174424), Nig01 (GenBank accession no. FJ174426), Ug03H.1 (GenBank accession no. FJ174431), Ug64 (GenBank accession no. FJ174430), Ken05.DPU1 (GenBank accession no. HM745354), Ken08WH/4 (GenBank accession no. HM745333), ken09Tk.20/5 (GenBank accession no. HM745344), Con09/PN003 (GenBank accession no. HQ645949), TAN/08/Mazimbu* (GenBank accession no. GQ410767), TAN/08/Mabibo* (GenBank accession no. GQ410768), Arm07 (GenBank accession no. JX857494), Az08D (GenBank accession no. JX857501), Oren08 (GenBank accession no. JX857498), Rostov09 (GenBank accession no. JX857504), Tver0312/Novo (GenBank accession no. KJ627190), Bel13/Grodno (GenBank accession no. KJ627192), LT14/1490 (GenBank accession no. KJ627193), ETH/1 (GenBank accession no. KT795366), ET13/1504 (GenBank accession no. KU291452), ETH/017 (GenBank accession no. KT795369), Ken06.Bus (GenBank accession no. KM111295), Ken07.Kia (GenBank accession no. FJ174437), CON09/Bzz020 (GenBank accession no. HQ645950), Ken10/KakFA1 (GenBank accession no. KC112568), Ug10.Kumi (GenBank accession no. KC990876), BUR/90/2 (GenBank accession no. KF015897), Ug12.Wakiso (GenBank accession no. KC990885), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/R7/2015 (GenBank accession no. MH025917), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894), CN201801 (GenBank accession no. MH735140), Estonia 2014 (GenBank accession no. LS478113), Nig6_JS10 (GenBank accession no. KT961344)으로부터 p54 (E183L) 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 3의 아미노산 서열 및 서열번호 13의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 C-type lectin (EP153R) 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 3의 아미노산 서열 및 서열번호 13의 염기 서열은 아프리카돼지열병 바이러스 균주 Katanga/63 (GenBank accession no. KM609340), Uganda (GenBank accession no. KM609361), BA71 (GenBank accession no. KP055815), Davis (GenBank accession no. KM609336), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), NH/P68 (GenBank accession no. AF481875), Mafra 86 (GenBank accession no. DQ026269), Coimbra 87 (GenBank accession no. DQ026268), OURT 88/3 (GenBank accession no. AM712240), Portalegre 90 (GenBank accession no. DQ026270), Barrancos 93 (GenBank accession no. DQ026267), Almodovar 99 (GenBank accession no. DQ026265), Almodovar 99/NE1 (GenBank accession no. DQ026266), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.Bus (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/R25/2015 (GenBank accession no. MH025918), Uganda/R8/2015 (GenBank accession no. MH025916), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_03029_C201 (GenBank accession no. MG939587), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894), UGA (GenBank accession no. AF017039), PR5 (GenBank accession no. AF017037), M1 (GenBank accession no. AF017034), K1 (GenBank accession no. AF017033), CR3 (GenBank accession no. AF017029), CR1 (GenBank accession no. AF017028), LC-PP (GenBank accession no. KM609345), Magadi (GenBank accession no. kM609348), Bartlett (GenBank accession no. KM609335), Volgograd_2012/wb (GenBank accession no. KM609363), Volgograd_2012/dom (GenBank accession no. KM609362), Tver_2012/wb (GenBank accession no. KM609360), Rhodesia (GenBank accession no. KM609354), TSP80 (GenBank accession no. KM609359), STP-1 (GenBank accession no. KM609355), O-77 (GenBank accession no. KM609350), F-32 (GenBank accession no. KM609337), MK-200 (GenBank accession no. KM609347), Spencer (GenBank accession no. KM609357), Silva-1 (GenBank accession no. kM609356), Ndjassi-77 (GenBank accession no. kM609349), KK-262 (GenBank accession no. KM609341), K-49 (GenBank accession no. KM609339), L-57 (GenBank accession no. KM609344), L-50 (GenBank accession no. KM609343)으로부터 C-type lectin (EP153R) 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 4의 아미노산 서열 및 서열번호 14의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 CD2v (EP402R) 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 4의 아미노산 서열 및 서열번호 14의 염기 서열은 아프리카돼지열병 바이러스 균주 China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894), Belgium 2018/1 (GenBank accession no. LR536725), Pol17_03029_C201 (GenBank accession no. MG939587), Pol16_20186_o7 (GenBank accession no. MG939583), Uganda/R8/2015 (GenBank accession no. MH025916), Uganda/N10/2015 (GenBank accession no. MH025919), Estonia 2014 (GenBank accession no. LS478113), Davis (1959_Kenya) (GenBank accession no. KM609336), Killean II (1959_Kenya) (GenBank accession no. KM609372), Kimakia II (1961_Kenya) (GenBank accession no. KM609374), Ba71V (1971_Spain) (GenBank accession no. U18466), E75 (1975_Spain) (GenBank accession no. FN557520), Ca78 (Italy_1978) (GenBank accession no. KT718663), Ori85 (Italy_1985) (GenBank accession no. KT718668), Nu91.5 (Italy_1991) (GenBank accession no. KT718673), NH/P68 (Portugal_1968) (GenBank accession no. AF481875), Mafra 86 (Portugal_1986) (GenBank accession no. DQ026269), Coimbra 87 (Portugal_1987) (GenBank accession no. DQ026268), Portalegre 90 (GenBank accession no. DQ026270), Barrancos 93 (GenBank accession no. DQ026267), Almodovar 99/NE1 (GenBank accession no. DQ026266), OURT 88/3 (GenBank accession no. AM712240), FR682468 (GenBank accession no. Georgia 2007), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.Bus (GenBank accession no. KM111295), Italy/47/SS/2008 (GenBank accession no. KX354450), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/26544/OG10 (GenBank accession no. KM102979), Benin 97/1 (GenBank accession no. AM712239), Bartlett (GenBank accession no. KM609335), Zavidovo-2012 (GenBank accession no. KM609392), Tver_2012/wb (GenBank accession no. KM609360), Rhodesia (GenBank accession no. KM609354), Nanyuki (GenBank accession no. KM609382), Krasnodar_2012/dom (GenBank accession no. KM609342), TS-7/27-230 (GenBank accession no. KM609388), TSP80 (GenBank accession no. KM609359), K-49 (GenBank accession no. KM609339), KK-262 (GenBank accession no. KM609341), Spencer (GenBank accession no. KM609357), MK-200 (GenBank accession no. KM609347), 691/88 (GenBank accession no. KM609334), F-32 (GenBank accession no. KM609337), O-77 (GenBank accession no. KM609350), P-60 (GenBank accession no. KM609351), PPA (GenBank accession no. KM609352), STP-1 (GenBank accession no. KM609355), BA71 (GenBank accession no. KP055815), STP-1-79 (GenBank accession no. KM609384), Malta (GenBank accession no. KM609380), Yamba-74 (GenBank accession no. KM609391), TKF (GenBank accession no. KM609387), Kimuele-6 (GenBank accession no. KM609375), E-70 (GenBank accession no. KM609369), Cuba-71 (GenBank accession no. KM609366), CU-80 (GenBank accession no. KM609365), Brazil-80 (GenBank accession no. KM609364), MNI-82 (GenBank accession no. KM609378), K-73 (Le Bray) (GenBank accession no. KM609370), Madeira (GenBank accession no. KM609379), Diamang (GenBank accession no. KM609367), L-50 (GenBank accession no. KM609343), L-57 (GenBank accession no. KM609344), Kikasa-77 (GenBank accession no. KM609371), VL (GenBank accession no. KM609390)로부터 CD2v (EP402R) 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 5의 아미노산 서열 및 서열번호 15의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 p49 (B438L) 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 5의 아미노산 서열 및 서열번호 15의 염기 서열은 아프리카돼지열병 바이러스 균주 BA71 (GenBank accession no. KP055815), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.BUS (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/N10/2015 (GenBank accession no. MH025919), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894)로부터 p49 (B438L) 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 6의 아미노산 서열 및 서열번호 16의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 pp62 (CP530R) 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 6의 아미노산 서열 및 서열번호 16의 염기 서열은 아프리카돼지열병 바이러스 균주 BA71 (GenBank accession no. KP055815), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.BUS (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/N10/2015 (GenBank accession no. MH025919), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894), Krasnodar (2012) (GenBank accession no. KJ380911)로부터 pp62 (CP530R) 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 7의 아미노산 서열 및 서열번호 17의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 EP364R 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 7의 아미노산 서열 및 서열번호 17의 염기 서열은 아프리카돼지열병 바이러스 균주 BA71 (GenBank accession no. KP055815), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.BUS (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/N10/2015 (GenBank accession no. MH025919), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894)로부터 EP364R 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 8의 아미노산 서열 및 서열번호 18의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 F317L 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 8의 아미노산 서열 및 서열번호 18의 염기 서열은 아프리카돼지열병 바이러스 균주 BA71 (GenBank accession no. KP055815), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.BUS (GenBank accession no. KM111295), Estonia 2014 F317L (GenBank accession no. LS478113), Benin 97/1 F317L (GenBank accession no. AM712239), Italy/26544/OG10 F317L (GenBank accession no. KM102979), Portugal/NHV/1968 F317L (GenBank accession no. KM262845), Italy/47/SS/2008 F317L (GenBank accession no. KX354450), Uganda/R35/2015 F317L (GenBank accession no. MH025920), Uganda/N10/2015 (GenBank accession no. MH025919), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894)로부터 F317L 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 9의 아미노산 서열 및 서열번호 19의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 A104R 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 9의 아미노산 서열 및 서열번호 19의 염기 서열은 아프리카돼지열병 바이러스 균주 BA71 (GenBank accession no. KP055815), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.BUS (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/N10/2015 (GenBank accession no. MH025919), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180), ASFV-SY18 (GenBank accession no. MH766894)로부터 A104R 유전자의 서열 상동성을 비교하여 제작하였다.
서열번호 10의 아미노산 서열 및 서열번호 20의 염기 서열은 아프리카돼지열병 바이러스의 다양한 균주들로부터 K205R 유전자의 서열 상동성을 비교하여 도출해낸 컨센서스(consensus) 서열이다. 구체적으로, 서열번호 10의 아미노산 서열 및 서열번호 20의 염기 서열은 아프리카돼지열병 바이러스 균주 BA71 (GenBank accession no. KP055815), Ba71V (GenBank accession no. U18466), E75 (GenBank accession no. FN557520), OURT 88/3 (GenBank accession no. AM712240), Georgia 2007 (GenBank accession no. FR682468), Ken05/Tk1 (GenBank accession no. KM111294), Ken06.BUS (GenBank accession no. KM111295), Estonia 2014 (GenBank accession no. LS478113), Benin 97/1 (GenBank accession no. AM712239), Italy/26544/OG10 (GenBank accession no. KM102979), Portugal/NHV/1968 (GenBank accession no. KM262845), Italy/47/SS/2008 (GenBank accession no. KX354450), Uganda/R35/2015 (GenBank accession no. MH025920), Uganda/N10/2015 (GenBank accession no. MH025919), Pol16_20186_o7 (GenBank accession no. MG939583), Pol17_04461_C210 (GenBank accession no. MG939588), Belgium 2018/1 (GenBank accession no. LR536725), China/2018/AnhuiXCGQ (GenBank accession no. MK128995), DB/LN/2018 (GenBank accession no. MK333181), Pig/HLJ/2018 (GenBank accession no. MK333180)ASFV-SY18 (GenBank accession no. MH766894)로부터 K205R 유전자의 서열 상동성을 비교하여 제작하였다.
실시예 2. 아프리카돼지열병 백신 제작
상기 실시예 1의 아프리카돼지열병 바이러스 유전자를 사용하여 백신을 제조하였다.
구체적으로, 당업계에 알려진 일반적인 방법으로 상기 유전자, Kozak 서열, IgE leader 서열, 유비퀴틴(ubiquitin) 서열 및/또는 퓨린 절단 부위(Furin cleavage site) 서열을 포함하는 DNA 플라스미드를 제조하였다. 이후, 상기 DNA 플라스미드를 보유한 대장균을 LB 배지 2.5L에 접종하고 37℃, 수중유적형 (oil-in-water, O/W) 배양을 수행하였다. 배양된 대장균으로부터 EndoFree Plasmid Giga kit (QIAGEN, Cat#12391)를 이용하여 플라스미드를 추출하였으며, 추출한 플라스미드는 차후 백신으로서 접종에 사용하였다.
상기 제조한 DNA 플라스미드의 개열 지도는 도 1 내지 5에 나타내었다.
실시예 3. 아프리카돼지열병 백신의 세포성 면역반응 유도 효과
상기 실시예 2에서 제조한 DNA 백신의 아프리카돼지열병 예방 효과로서, 세포성 면역반응 (CTL, Cytotoxic T Lymphocyte response) 유도 효과를 확인하였다.
C57BL/6 마우스 (8주령, Female) 15마리를 그룹당 5마리씩 총 3 그룹으로 나눈 뒤 2주 간격으로 백신 접종을 3번 실시하였다. 투여한 DNA 백신의 농도는 30ug/마리이며, 전기천공(electroporation) 기기(cellectra 2000)를 이용하여 투여 및 접종을 실시하였다. 이때, 각 대조군 및 실험군에 투여한 DNA 백신의 종류는 아래와 같이 설정하였다.
- 대조군: 아프리카돼지열병 바이러스 유전자가 포함되지 않은 DNA 백신 (Mock Plasmid DNA vaccine)
- 실험군 1: 아프리카돼지열병 바이러스 유전자를 포함하며, 유비퀴틴을 포함하지 않는 DNA 백신 (ASF_4G DNA vaccine; p30, p54, C type lectin 및 CD2v)
- 실험군 2: 아프리카돼지열병 바이러스 유전자를 포함하며, 유비퀴틴을 포함하는 DNA 백신 (ASF_Ubi_4G DNA vaccine; 유비퀴틴, p30, p54, C type lectin 및 CD2v)
상기 DNA 백신을 3회 투여한 후, 일주일 뒤에 마우스에서 비장(spleen)을 적출하여 비장세포(splenocyte)를 분리하였고, 분리한 비장세포에 대하여 IFN-gamma ELISpot KIT(Cellular Technology Limited/미국)를 사용하여 mouse IFN-gamma ELISpot 분석을 수행하였다. 구체적으로, 각 백신이 투여된 상기 비장세포에 각 항원 유전자의 폴리펩티드를 처리한 후 IFN-γ를 분비하는 세포독성 T 세포(CD8+ T 세포)의 수를 분석함으로써 백신 접종 후 바이러스 감염에 의해 유도되는 세포성 면역반응의 정도를 확인하였다.
그 결과, 도 6 및 하기 표 1에서 볼 수 있듯이, 아프리카돼지열병 바이러스 유전자를 포함하는 DNA 백신이 투여된 실험군 1 및 2에서는 각 항원 유전자의 폴리펩티드가 처리되는 경우, IFN-γ를 분비하는 세포독성 T 세포(CD8+ T 세포)의 수가 현저하게 증가함을 확인하였다.
p30 p54 CD2v
대조군 (Mock) 0.0 0.0 8.5
실험군 1 (ASF_4G) 0.0 769.0 336.5
실험군 2 (ASF_Ubi_4G) 23 1223.5 764
(단위: SFU/106 세포)
특히, 유비퀴틴을 포함하지 않는 DNA 백신(실험군 1)이 투여된 경우에 비하여, 유비퀴틴을 포함하는 DNA 백신이 투여된 경우(실험군 2)에서는 IFN-γ를 분비하는 세포독성 T 세포(CD8+ T 세포)의 수가 약 약 1.6배 내지 2.2배 증가됨을 확인하였다. 또한, DNA 백신에 포함된 다양한 항원 유전자 중에서도 p54 또는 CD2v에 의해 IFN-γ를 분비하는 세포독성 T 세포(CD8+ T 세포)의 수가 특히 증가되며, p30에 의해서는 유비퀴틴을 포함하는 백신이 투여된 경우(실험군 2)에만 IFN-γ를 분비하는 세포독성 T 세포(CD8+ T 세포)의 수가 증가함을 확인하였다.
상기 결과를 통해, 아프리카돼지열병 바이러스 유전자와 유비퀴틴을 포함하는 DNA 백신을 접종한 경우, 아프리카돼지열병 바이러스에 감염되더라도 IFN-γ를 분비하는 세포독성 T 세포의 수를 현저히 증가시키는 등의 세포성 면역반응을 효율적으로 유도할 수 있으므로, 상기 DNA 백신은 아프리카돼지열병 예방을 위한 백신 조성물로서 유용하게 활용될 수 있음을 알 수 있었다.
실시예 4. 아프리카돼지열병 백신의 생존율 증가 효과
상기 실시예 2에서 제조한 백신의 아프리카돼지열병 예방 효과로서, 아프리카돼지열병 바이러스 감염 이후의 생존율 증가 효과를 확인하였다.
구체적으로, 자돈 돼지에 상기 DNA 백신을 총 3회 (0주차, 2주차, 4주차) 투여하였다. 이후, 7주차에 아프리카돼지열병 바이러스를 공격접종(challenge)하였고, 이후 28일간의 생존율을 조사하였다. 이때, 각 대조군 및 실험군에 투여한 DNA 백신의 종류는 아래와 같이 설정하였다.
- 대조군: 아프리카돼지열병 바이러스 유전자가 포함되지 않은 DNA 백신 (Mock)
- 실험군: 아프리카돼지열병 바이러스 유전자를 포함하며, 유비퀴틴을 포함하는 DNA 백신 (ASF_Ubi_10G DNA vaccine; 유비퀴틴, p30, p54, C-type lectin, CD2v, p49, pp62, EP364R, F317L, A104R 및 K205R )
그 결과, 도 7에서 볼 수 있듯이, 대조군의 경우, 접종 28일차에는 3마리 중 1마리의 돼지만이 생존하여 약 33%의 낮은 생존율을 나타냄을 확인하였다. 반면에, 아프리카돼지열병 바이러스 유전자를 포함하는 DNA 백신이 투여된 실험군의 경우, 접종 28일차에는 6마리 중 4마리의 돼지가 생존하여 약 66%의 높은 생존율을 나타내며, 대조군에 비하여 생존율이 2배 이상 증가함을 확인하였다.
상기 결과를 통해, 아프리카돼지열병 바이러스 유전자와 유비퀴틴을 포함하는 DNA 백신을 접종한 경우, 아프리카돼지열병 바이러스에 감염되더라도 우수한 생존능을 나타내므로, 상기 DNA 백신은 아프리카돼지열병 예방을 위한 백신 조성물로서 유용하게 활용될 수 있음을 알 수 있었다.
<110> Plumbline Life Sciences, Inc. <120> VACCINE COMPOSITION FOR AFRICAN SWINE FEVER <130> DP2188KR <150> KR 10-2020-0045271 <151> 2020-04-14 <160> 54 <170> KoPatentIn 3.0 <210> 1 <211> 193 <212> PRT <213> Artificial Sequence <220> <223> ASFV p30 <400> 1 Asp Phe Ile Leu Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr 1 5 10 15 Asp Leu Arg Ser Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr 20 25 30 Asn Trp Phe Ser Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr 35 40 45 Ala Ile Lys Thr Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser 50 55 60 Ala Arg Ile Tyr Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu 65 70 75 80 Glu Trp Asn Met Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser 85 90 95 Ser Ala Ser Ser Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn 100 105 110 Glu Cys Thr Ser Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser 115 120 125 Glu Thr Pro Lys Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val 130 135 140 Gln His Ile Glu Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile 145 150 155 160 Arg Ala His Asn Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu 165 170 175 Glu Glu Lys Glu Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys 180 185 190 Lys <210> 2 <211> 184 <212> PRT <213> Artificial Sequence <220> <223> ASFV P54_EP183L <400> 2 Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu Cys 1 5 10 15 Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr Thr 20 25 30 Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile Ile Val Leu Ile 35 40 45 Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu Glu 50 55 60 Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala Glu 65 70 75 80 Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr Ala 85 90 95 Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro Ala 100 105 110 Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val Met 115 120 125 Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro Ala 130 135 140 His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala Ser 145 150 155 160 Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr Thr 165 170 175 His Lys Asp Leu Glu Asn Ser Leu 180 <210> 3 <211> 157 <212> PRT <213> Artificial Sequence <220> <223> ASFV EP153R <400> 3 Phe Ser Lys Lys Lys Tyr Ile Gly Leu Ile Asp Lys Lys Cys Glu Leu 1 5 10 15 Lys Lys Ile Leu Asp Asp Tyr Ser Ile Leu Ile Ile Gly Ile Leu Ile 20 25 30 Gly Thr Asn Ile Ile Thr Leu Ile Tyr Asn Phe Ile Gly Trp Ile Asn 35 40 45 Tyr Ile Lys Cys Tyr Gln Asn Asp Asp Lys Met Phe Tyr Cys Pro Lys 50 55 60 Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr Phe Ser Asn Glu Glu 65 70 75 80 Lys Asn Tyr Thr Asn Ala Ser Asn Tyr Cys Lys Gln Leu Asn Ser Thr 85 90 95 Leu Ala Asn Asn Asn Thr Asn Leu Val Asn Leu Thr Lys Thr Leu Asn 100 105 110 Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr Trp Val Asn Tyr Ser 115 120 125 Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asn Ser Gly Tyr Tyr Lys 130 135 140 Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys Ser Lys 145 150 155 <210> 4 <211> 364 <212> PRT <213> Artificial Sequence <220> <223> ASFV CD2v_EP402R <400> 4 Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn Ile Val Leu Ser Ile Ile 1 5 10 15 Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile Leu Asp Ser Asn Ile Thr 20 25 30 Asn Asp Asn Asn Asp Ile Asn Gly Val Ser Trp Asn Thr Tyr Phe Asn 35 40 45 Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly Lys Ala Gly Asn Phe Cys 50 55 60 Glu Cys Ser Asn Tyr Ser Thr Ser Leu Tyr Asn Ile Thr Asn Asn Cys 65 70 75 80 Ser Leu Thr Ile Phe Pro His Asn Thr Asp Ile Phe Asn Thr Thr Tyr 85 90 95 Gln Leu Val Tyr Leu Asn Gln Lys Ile Asn Tyr Thr Ile Lys Leu Leu 100 105 110 Thr Pro Val Asp Ser Pro Asn Ile Thr Tyr Asn Cys Thr Asn Ser Leu 115 120 125 Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn Thr Asn Ile Tyr Leu Asn 130 135 140 Ile Asn Asp Thr Ile Val Asn Tyr Thr Asn Glu Ser Ile Leu Asn Tyr 145 150 155 160 Tyr Trp Asn Asn Ser Asn Ile Asn Asn Phe Thr Ala Thr Cys Ile Ile 165 170 175 Asn Asn Thr Ile Ser Ser Ser Asn Glu Thr Glu Leu Ile Asn Cys Thr 180 185 190 Asn Pro Thr Leu Lys Tyr Gln Asn Tyr Phe Tyr Thr Phe Phe Lys Leu 195 200 205 Phe Tyr Ile Ile Ile Phe Ile Val Ser Gly Ile Ile Ile Ser Ile Leu 210 215 220 Phe Ile Ser Ile Ile Thr Phe Leu Ser Leu Arg Lys Arg Lys Lys His 225 230 235 240 Val Glu Glu Ile Glu Ser Pro Pro Pro Ser Glu Ser Asn Glu Glu Glu 245 250 255 Gln Cys Gln His Asp Asp Thr Thr Ser Ile His Glu Pro Ser Pro Arg 260 265 270 Glu Pro Leu Leu Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn Thr Pro 275 280 285 Ile Tyr Tyr Met Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe Pro Leu 290 295 300 Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys 305 310 315 320 Pro Pro Pro Lys Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro Pro Lys 325 330 335 Pro Leu Pro Ser Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu Ser Thr 340 345 350 Gln Asn Ile Ser Leu Ile His Val Asp Arg Ile Ile 355 360 <210> 5 <211> 437 <212> PRT <213> Artificial Sequence <220> <223> ASFV p49_B438L <400> 5 Tyr His Asp Tyr Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro 1 5 10 15 Pro Leu Trp Glu Ser Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn 20 25 30 Ile Leu Asn Ser Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val 35 40 45 Tyr Asn Glu Tyr Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln 50 55 60 Leu Ser Asp Leu Arg Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys 65 70 75 80 Asn Ile Gly Tyr Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile 85 90 95 Pro Lys Asn Lys Leu Phe Asn His Phe Lys Asn His Arg Pro Ala Phe 100 105 110 Ser Thr His Thr Glu Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu 115 120 125 Lys Lys Thr Phe Pro Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn 130 135 140 Cys Leu Thr Gln Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn 145 150 155 160 Ser Ser Val Pro Ser Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser 165 170 175 Tyr Glu Asp Leu Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu 180 185 190 Gln His Leu Gly Asp Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile 195 200 205 Pro His Lys Asp Val Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro 210 215 220 Ala Ser Val Glu Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn 225 230 235 240 Pro His Val Lys Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser 245 250 255 Thr Tyr Thr Asp Val Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser 260 265 270 Gln His Gly Arg Glu Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu 275 280 285 Gln Val Ser Asn Ile Leu His Ser Val Phe Ala Asn His Ser Ile Lys 290 295 300 Pro Gln Ile Leu Glu Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser 305 310 315 320 Ile Lys Pro Val Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr 325 330 335 Met Glu Asn Ile Val Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn 340 345 350 Tyr Ser Ser Pro Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala 355 360 365 Glu Tyr Phe Asp Thr Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr 370 375 380 Ile Pro Lys Tyr Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr 385 390 395 400 Phe Pro Lys Asp Phe Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu 405 410 415 Lys Asp His Ser Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser 420 425 430 Ile Ser Pro Ser Leu 435 <210> 6 <211> 529 <212> PRT <213> Artificial Sequence <220> <223> ASFV pp62_CP530R <400> 6 Pro Ser Asn Met Lys Gln Phe Cys Lys Ile Ser Val Trp Leu Gln Gln 1 5 10 15 His Asp Pro Asp Leu Leu Glu Ile Ile Asn Asn Leu Cys Met Leu Gly 20 25 30 Asn Leu Ser Ala Ala Lys Tyr Lys His Gly Val Thr Phe Ile Tyr Pro 35 40 45 Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys Lys His Ala Tyr Ser Asn 50 55 60 Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu Ser Leu Ile Leu Pro Phe 65 70 75 80 Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly Glu Ile Gly Ser Tyr Thr 85 90 95 Gly Val Lys Leu Glu Val Glu Lys Thr Glu Ala Asn Lys Val Ile Leu 100 105 110 Lys Asn Gly Glu Ala Val Leu Val Pro Ala Ala Asp Phe Lys Pro Phe 115 120 125 Pro Asp Arg Arg Leu Ala Val Trp Ile Met Glu Ser Gly Ser Met Pro 130 135 140 Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys Glu Gly Gly Gly Asn Asp 145 150 155 160 Pro Pro Val Pro Lys His Ile Ser Pro Tyr Thr Pro Arg Thr Arg Ile 165 170 175 Ala Ile Glu Val Glu Lys Ala Phe Asp Asp Cys Met Arg Gln Asn Trp 180 185 190 Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys Ser Val Ser Leu Leu Ser 195 200 205 Phe Leu Ser Leu Asn His Pro Thr Glu Phe Ile Lys Val Leu Pro Leu 210 215 220 Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr Leu Leu Leu Glu Pro Tyr 225 230 235 240 Lys Thr His Gly Asp Asp Phe Leu Ile Pro Glu Thr Ile Leu Phe Gly 245 250 255 Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr Gln Ser Ala Met Leu Glu 260 265 270 Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg Gln Thr Phe Met Asp Ile 275 280 285 Ala Asp Ser Ala Thr Lys Glu Val Asp Val Pro Ile Cys Tyr Ser Asp 290 295 300 Pro Glu Thr Val His Ser Tyr Ala Asn His Val Arg Thr Glu Ile Leu 305 310 315 320 His His Asn Ala Val Asn Lys Val Thr Thr Pro Asn Leu Val Val Gln 325 330 335 Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr Ile Arg His Tyr Gly Pro 340 345 350 Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu Arg Phe Trp Lys Lys Leu 355 360 365 Trp Gln Asp Glu Gln Arg Phe Val Ile His Gly Leu His Arg Thr Leu 370 375 380 Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu Phe Ala Glu Ile Val Arg 385 390 395 400 Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn Tyr Ile Asn Glu Leu Asn 405 410 415 Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys His Thr Thr Gly Asp Ile 420 425 430 Ala Pro Asn Asp Arg Phe Ala Met Leu Val Ala Phe Ile Asn Ser Thr 435 440 445 Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu Lys Val Gly Gly Asn Glu 450 455 460 Thr Gln Thr Ser Ser Leu Thr Asp Leu Val Pro Thr Arg Leu His Ser 465 470 475 480 Phe Leu Asn His Asn Leu Ser Lys Leu Lys Ile Leu Asn Arg Ala Gln 485 490 495 Gln Thr Val Arg Asn Ile Leu Ser Asn Asp Cys Leu Asn Gln Leu Lys 500 505 510 His Tyr Val Lys His Thr Gly Lys Asn Glu Ile Leu Lys Leu Leu Gln 515 520 525 Glu <210> 7 <211> 368 <212> PRT <213> Artificial Sequence <220> <223> ASFV EP364R <400> 7 Tyr Phe Leu Val Ala Asp His Arg Glu His His Val Ile Pro Phe Leu 1 5 10 15 Lys Thr Asp Phe His His Met His Gln Asn Pro Ile Gln Lys Lys Gln 20 25 30 Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr Gly Asp Tyr Leu Ile Cys 35 40 45 Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile Glu Arg Lys Thr Tyr Lys 50 55 60 Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg Tyr Lys Asn Arg Gln Lys 65 70 75 80 Met Leu Ser Leu Arg Glu Gln Thr Asn Cys Gln Leu Tyr Phe Phe Val 85 90 95 Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys Lys Ile Asn His Val Ala 100 105 110 Tyr Ala Ser Ile Ile Thr Ala Met Thr His Leu Met Val Arg Asp His 115 120 125 Ile Phe Val Ile Gln Thr Lys Asn Glu Ala His Ser Ser Gln Lys Leu 130 135 140 Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu Met Val Cys Val Val Pro 145 150 155 160 Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu Cys Ile Lys Leu Trp Ser 165 170 175 Ser Leu Ser Gly Ile Ser Gly Val Ile Gly Lys Ile Leu Ala Asn Thr 180 185 190 Cys Ser Val Ala His Leu Val Ser Gly Lys Leu Ser Ser Gln Asn Ile 195 200 205 Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro Phe Pro Lys Lys Val Lys 210 215 220 Arg Met Leu Ile Ser Ile Ser Lys Gly Asn Lys Glu Leu Glu Ile Lys 225 230 235 240 Leu Leu Ser Gly Val Pro Asn Ile Gly Lys Lys Leu Ala Ala Glu Ile 245 250 255 Leu Lys Asp His Ala Leu Leu Phe Phe Leu Asn Gln Pro Val Glu Cys 260 265 270 Leu Ala Asn Ile Gln Ile Val Gln Lys Thr Arg Thr Ile Lys Leu Gly 275 280 285 Met Lys Arg Ala Glu Ala Ile His Tyr Phe Leu Asn Trp Cys Gly Ser 290 295 300 Ala His Val Thr Asp Asp Ser Gln Asn Ile Thr Glu Ala Ser Arg Pro 305 310 315 320 Thr Met Gln Val Ala Thr Gln Ser Ala Ala Thr Gln Pro Ala Ala Thr 325 330 335 Gln Pro Leu His Glu Val Ser Asp Asp Ala Ser Ser Asp Ala Ser Ser 340 345 350 Pro Val Gly His Gln Thr Leu Ser Lys Glu Met Leu Leu Asn Thr Ala 355 360 365 <210> 8 <211> 316 <212> PRT <213> Artificial Sequence <220> <223> ASFV F317L <400> 8 Val Glu Thr Gln Met Asp Lys Leu Gly Phe Leu Leu Asn His Ile Gly 1 5 10 15 Lys Gln Val Thr Thr Lys Val Leu Ser Asn Ala His Ile Thr Gln Thr 20 25 30 Met Lys Glu Ile Ile Leu Glu Asn His Ser Val Asp Gly Gly Ala Ala 35 40 45 Lys Asn Val Ser Lys Gly Lys Ser Ser Pro Lys Glu Lys Lys His Trp 50 55 60 Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys Ser Lys Arg Ser Phe 65 70 75 80 Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val Asn Thr Leu Leu Leu 85 90 95 Asn Trp Asp Asn Val Arg Gly Ala Ile Lys Lys Phe Leu Asp Asp Asp 100 105 110 Arg Glu Trp Cys Gly Arg Ile Asn Met Ile Asn Gly Val Pro Glu Ile 115 120 125 Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly Glu Asn Ile Tyr Phe 130 135 140 Gly Ser Glu Ala Met Met Pro Ala Asp Ile Tyr Ser Arg Val Ala Asn 145 150 155 160 Lys Pro Ala Met Phe Val Phe His Thr His Pro Asn Leu Gly Ser Cys 165 170 175 Cys Gly Gly Met Pro Ser Ile Cys Asp Ile Ser Thr Thr Leu Arg Tyr 180 185 190 Leu Leu Met Gly Trp Thr Ala Gly His Leu Ile Ile Ser Ser Asn Gln 195 200 205 Val Gly Met Leu Thr Val Asp Lys Arg Ile Ile Val Asp Leu Trp Ala 210 215 220 Asn Glu Asn Pro Arg Trp Leu Met Ala Gln Lys Ile Leu Asp Ile Phe 225 230 235 240 Met Met Leu Thr Ser Arg Arg Ser Leu Val Asn Pro Trp Thr Leu Arg 245 250 255 Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile Glu Tyr Ile Ile Phe 260 265 270 Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu Arg Leu Leu Met Phe 275 280 285 Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu His Glu Leu Leu Asp Asp 290 295 300 Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr Thr 305 310 315 <210> 9 <211> 103 <212> PRT <213> Artificial Sequence <220> <223> ASFV A104R <400> 9 Ser Thr Lys Lys Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr Ser Leu 1 5 10 15 Val Ala Ala Asp Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg Ile Phe 20 25 30 Thr Ser Gln Gln Lys Ile Ile Gln Asn Ala Leu Lys His Asn Gln Glu 35 40 45 Val Ile Ile Pro Pro Gly Ile Lys Phe Thr Val Val Thr Val Lys Ala 50 55 60 Lys Pro Ala Arg Gln Gly His Asn Pro Ala Thr Gly Glu Pro Ile Gln 65 70 75 80 Ile Lys Ala Lys Pro Glu His Lys Ala Val Lys Ile Arg Ala Leu Lys 85 90 95 Pro Val His Asp Met Leu Asn 100 <210> 10 <211> 204 <212> PRT <213> Artificial Sequence <220> <223> ASFV K205R <400> 10 Val Glu Pro Arg Glu Gln Phe Phe Gln Asp Leu Leu Ser Ala Val Asp 1 5 10 15 Gln Gln Met Asp Thr Val Lys Asn Asp Ile Lys Asp Ile Met Lys Glu 20 25 30 Lys Thr Ser Phe Met Val Ser Phe Glu Asn Phe Ile Glu Arg Tyr Asp 35 40 45 Thr Met Glu Lys Asn Ile Gln Asp Leu Gln Asn Lys Tyr Glu Glu Met 50 55 60 Ala Ala Asn Leu Met Thr Val Met Thr Asp Thr Lys Ile Gln Leu Gly 65 70 75 80 Ala Ile Ile Ala Gln Leu Glu Ile Leu Met Ile Asn Gly Thr Pro Leu 85 90 95 Pro Ala Lys Lys Thr Thr Ile Lys Glu Ala Met Pro Leu Pro Ser Ser 100 105 110 Asn Thr Asn Asn Glu Gln Thr Ser Pro Pro Ala Ser Gly Lys Thr Ser 115 120 125 Glu Thr Pro Lys Lys Asn Pro Thr Asn Ala Met Phe Phe Thr Arg Ser 130 135 140 Glu Trp Ala Ser Ser Asn Thr Phe Arg Glu Lys Phe Leu Thr Pro Glu 145 150 155 160 Ile Gln Ala Ile Leu Asp Glu Gln Phe Ala Asn Lys Thr Gly Ile Glu 165 170 175 Arg Leu His Ala Glu Gly Leu Tyr Met Trp Arg Thr Gln Phe Ser Asp 180 185 190 Glu Gln Lys Lys Met Val Lys Glu Met Met Lys Lys 195 200 <210> 11 <211> 579 <212> DNA <213> Artificial Sequence <220> <223> ASFV p30_CP204L <400> 11 gattttattt taaatatatc catgaaaatg gaggtcatct tcaaaacgga tttaagatca 60 tcttcacaag ttgtgtttca tgcgggtagc ctgtataatt ggttttctgt tgagattatc 120 aatagcggta gaattgttac gaccgctata aaaacattgc ttagtactgt taagtatgat 180 attgtgaaat ctgctcgtat atatgcaggg caagggtata ctgaacatca ggctcaagaa 240 gaatggaata tgattctgca tgtgctgttt gaagaggaga cggaatcctc agcatcttcg 300 gagtcaattc atgaaaaaaa tgataatgaa accaatgaat gcacatcctc ctttgaaacg 360 ttgtttgagc aagagccctc atcggagaca cctaaagact ccaagctgta tatgcttgca 420 caaaagactg tgcaacatat tgaacaatat ggaaaggcac ctgattttaa caaggttatt 480 agagcacata attttattca aaccattcat ggaacccctc taaaggaaga agaaaaagag 540 gtggtaagac tcatggttat taaactttta aaaaaaaaa 579 <210> 12 <211> 552 <212> DNA <213> Artificial Sequence <220> <223> ASFV P54_EP183L <400> 12 gattctgaat tttttcaacc ggtttatccg cggcattatg gtgagtgttt gtcaccagtc 60 actacaccaa gcttcttctc cacacatatg tatactattc tcattgctat cgtggtctta 120 gtcatcatta tcatcgttct aatctatcta ttctcttcaa gaaagaaaaa agctgctgct 180 gctattgagg aggaagatat acagtttata aatccttatc aagatcagca gtgggcagaa 240 gtcactccac aaccaggtac ctctaaacca gctggagcga ctacagcaag tgtaggcaag 300 ccagtcacgg gcagaccggc aacaaacaga ccagcaacaa acaaaccagt tacggacaac 360 ccagttacgg acagactagt catggcaact ggcgggccgg cggccgcacc tgcggccgcg 420 agtgctcctg ctcatccggc tgagccttac acgacagtca ctactcagaa cactgcttca 480 caaacaatgt cggctattga aaatttacga caaagaaaca cctatacgca taaagaccta 540 gaaaactcct tg 552 <210> 13 <211> 471 <212> DNA <213> Artificial Sequence <220> <223> ASFV C-type lectin_EP153R <400> 13 ttttctaaga aaaagtacat cggtcttatc gataagaagt gtgaattgaa aaaaatacta 60 gatgattata gtatattaat aattggaata ttaattggaa ctaacatcat aacccttatt 120 tataatttta taggatggat taattatata aaatgttacc aaaatgatga taagatgttt 180 tattgcccta aagattgggt tggatataat aatgtttgtt attattttag taatgaagaa 240 aaaaattata ctaatgcaag taattattgt aagcaattaa atagtacgct tgctaataat 300 aatactaatt tagtaaatct tactaaaaca ttaaatctta ctaaaacata taatcacgaa 360 tctaattatt gggttaatta ttctttaatt aaaaatgagt cagtactatt acgtaatagt 420 ggatattaca aaaaacaaaa acatgtaagt ttattatata tttgtagtaa a 471 <210> 14 <211> 1092 <212> DNA <213> Artificial Sequence <220> <223> ASFV CD2v_EP402R <400> 14 ataatactta tttttttaat attttctaac atagttttaa gtattatata ttgggttagt 60 tttaataaaa caataatttt agatagtaat attactaatg ataataatga tataaatgga 120 gtatcatgga atacttattt taataattct tttaatacac tagctacatg tggaaaagca 180 ggtaactttt gtgaatgttc taattatagt acatcactat ataatataac aaataattgt 240 agcttaacta tttttcctca taatactgat atatttaata caacatatca actagtatat 300 ctaaatcaaa aaattaatta tacaataaaa ttattaacac ctgtagattc tccaaatatc 360 acatataatt gtactaattc tttaataaca tgtaaaaaaa ataatggaac aaacactaat 420 atatatttaa atataaatga tactatagtt aattatacta atgaaagtat acttaattat 480 tattggaata atagtaacat taacaatttt acagctacat gtataattaa taatacaatt 540 agttcttcta atgaaacaga acttataaat tgtactaatc ccacattgaa atatcaaaac 600 tatttttata ctttttttaa attattttat attataatat ttatcgtatc tgggataata 660 ataagtattc tttttatatc catcataact tttttatctt tacgaaaaag aaaaaaacat 720 gttgaagaaa tagaaagtcc accaccttct gaatctaatg aagaagaaca atgtcagcat 780 gatgacacca cttccataca tgaaccatct cccagagaac cattacttcc taagccttac 840 agtcgttatc agtataatac acctatttac tacatgcgtc cctcaacaca accactcaac 900 ccatttccct tacctaaacc gtgtcctcca cccaaaccat gtccgccacc caaaccatgt 960 cctccaccta aaccatgtcc ttcagctgaa tcctattctc cacccaaacc actacctagt 1020 atcccgctac tacccaatat cccgccatta tctacccaaa atatttcgct tattcacgta 1080 gatagaatta tt 1092 <210> 15 <211> 1311 <212> DNA <213> Artificial Sequence <220> <223> ASFV p49_B438L <400> 15 tatcatgatt atgcttcaaa gctgttagcc gattataggt cagatccacc tctttgggaa 60 agcgatttgc cgcgtcataa ccggtattct gataatatac taaattcccg gtactgcggc 120 aacaaaaatg gagctgcgcc tgtatacaat gaatacacta atagtcctga aaaggctgaa 180 aagggattgc aactcagtga tctacgtaac ttttctttta tgctgaaccc tcagcacaaa 240 aacattggtt acggcgatgc gcaagatctg gagccgtaca gctccattcc aaaaaataaa 300 ctttttaatc attttaaaaa tcatcgtcct gctttttcaa cccatacgga aaacctaatt 360 aggcgcaatg ttgttcgtac ggaaaaaaaa acatttccgc aggtggctag cttaaagggc 420 acacagaaaa attgtctgac ccagccttcc tcattgcctt ccttaaaaaa cccaaaaaat 480 agttcggttc cttctacgag gttcagcgag cacacaaaat ttttttcgta cgaggatctt 540 cctaaactca gaacaaaggg taccattaag catgagcagc accttggcga tcagatgccg 600 ggccagcact ataacggata tattccgcac aaagacgtgt acaatatttt gtgtttagcg 660 cacaatctac ctgcgtcggt agaaaaaggg attgcaggta gaggtattcc tttaggaaat 720 ccacatgtca aaccgaacat agagcaggaa ctcataaaat ccaccagtac ctacacggac 780 gtgccgatgc ttggtcccct tccgcccaaa gattcgcaac acggcagaga ataccaggaa 840 ttttccgcaa atagacatat gctacaagta tctaatatac tacattctgt gtttgctaat 900 cattctataa agccacaaat cctagaagac ataccggtac tcaatgcaca actaacaagc 960 ataaagccgg taagcccatt tttaaacaaa gcgtatcaaa cccattatat ggaaaatatt 1020 gttaccttgg tgccccggtt taagagtatt gccaactact caagccctat accgaattat 1080 tctaaaagaa atagcggtca ggctgaatat tttgatacct caaagcaaac aatttctaga 1140 cacaataatt atatacctaa atatacggga ggaattggag attccaaact agacagcacc 1200 tttcctaaag atttcaacgc ctcctcggta ccgttaacaa gcgcggaaaa ggaccattcc 1260 ctgcggggag acaactcagc atgttgcatc tcatctatat ctccatcatt g 1311 <210> 16 <211> 1587 <212> DNA <213> Artificial Sequence <220> <223> ASFV pp62_CP530R <400> 16 ccctctaata tgaaacagtt ttgcaagatt tctgtatggc tacagcagca cgatccagat 60 ttattagaaa ttatcaacaa cttatgtatg cttggcaatt tatccgcggc aaagtacaaa 120 cacggagtta ccttcattta ccccaaacag gcaaagatcc gcgatgaaat aaaaaaacat 180 gcctactcca atgacccttc acaagccata aagaccttag aatcactcat ccttccattt 240 tacattccca ctccagcgga gttcaccggg gaaatcggct cctacaccgg agtgaaatta 300 gaggttgaaa aaacggaggc gaataaagtt attttaaaaa atggagaagc ggtcctagta 360 ccggcggccg attttaagcc ctttcctgat cgccgactag cggtctggat catggagtca 420 ggctctatgc ccctggaggg tcccccctat aagcggaaaa aggagggtgg ggggaatgac 480 ccgccggttc ctaagcatat ctcgccgtat actccgcgca cgcgtattgc cattgaggtg 540 gaaaaggcct ttgatgactg tatgcgtcaa aactggtgta gtgtcaataa tccctatctt 600 gccaagtcgg tctccttgct gtctttcttg tcgctcaacc atcccaccga gtttattaag 660 gtactgccgc ttatagactt tgaccccttg gtgacctttt atctacttct tgagccctat 720 aaaacgcatg gggatgactt tttaattccg gaaaccattt tattcggccc taccggatgg 780 aatggtacag atctgtatca aagtgccatg ctggagttta aaaagttttt tacccagatt 840 actcgccaaa cctttatgga catagccgat tcggctacta aggaggtaga tgttcccata 900 tgttactcgg atcccgaaac cgtacattcc tatgccaatc acgtgcgtac tgaaattttg 960 catcacaatg ccgtcaataa ggttacaaca cctaacctcg tcgtgcaggc ctataatgag 1020 ctcgagcaaa ccaataccat acgacattac ggccctattt tcccggaaag taccatcaac 1080 gcactgcgtt tttggaaaaa gctgtggcag gatgaacagc gatttgttat ccacggcctg 1140 caccgcacgt tgatggatca acccacctat gaaacctctg agtttgcaga gatcgttaga 1200 aatttacggt tttcgcgtcc cggcaataac tatataaacg agcttaatat tacaagtccc 1260 gctatgtacg gcgacaagca taccaccgga gatattgcgc ccaatgatag atttgccatg 1320 ttggtggcct ttatcaacag tactgacttt ttatacaccg cgattcccga ggaaaaggta 1380 ggggggaatg aaacccaaac cagtagcctt acagacctag ttccaacacg gctacactct 1440 tttttaaatc ataatctaag caaacttaaa atcttaaacc gcgcgcagca aacggttaga 1500 aatattcttt caaatgattg tcttaatcaa ctgaaacatt atgttaaaca cacgggaaaa 1560 aatgaaatac taaagttact tcaagaa 1587 <210> 17 <211> 1104 <212> DNA <213> Artificial Sequence <220> <223> ASFV EP364R <400> 17 tattttctag tagcagatca tcgagaacat catgtgattc cttttcttaa aaccgatttc 60 catcacatgc atcaaaatcc tatacaaaaa aaacaagctc tcctagaaat caaacagctt 120 tttactggag attatctcat ctgcaaaagc ccttctacca ttctggcctg tattgaacga 180 aaaacctaca aagactttgc ggcttctttg aaagatggac gttataaaaa tcgccaaaaa 240 atgctgtcgc tgcgagaaca aaccaactgt caactttatt tttttgtaga aggcccggca 300 tttcctaacc ctcaaaaaaa aattaatcac gttgcctatg caagcattat tactgctatg 360 acgcatctta tggttagaga tcatattttt gtcattcaaa cgaaaaatga ggcccacagt 420 tcccaaaagc ttgtgcagct tttttatgcc ttttctaagg aaatggtgtg cgtcgttccc 480 acctccctca cccccacgga tgaagagcta tgcatcaagc tatggtcttc tctttctggt 540 atttcaggcg tgataggtaa aatcttggca aacacttgtt ccgtagctca tttggtttct 600 ggaaagcttt catcgcagaa tattgatcag ttaaaaactc cctccaaccg accattcccc 660 aaaaaagtaa aacgtatgct tataagcatt agcaaaggaa ataaggagtt agaaataaaa 720 ttgctctcgg gggttcccaa tatcgggaaa aaattagctg ccgaaatttt aaaagatcat 780 gcgcttcttt tttttctaaa tcagcccgta gaatgcttgg caaatataca aatcgttcaa 840 aaaacccgta cgattaagtt gggaatgaag cgagccgaag cgattcatta ttttttaaac 900 tggtgtggct ctgcccatgt aaccgatgat agccaaaata tcacagaggc gtcgcggcct 960 acaatgcagg tcgcgacgca gtccgccgca actcagcccg ctgcaacgca gccattgcac 1020 gaagtatcag atgatgcatc atcagatgct tcatcacccg tagggcatca aacattatct 1080 aaagaaatgt tattgaacac agcc 1104 <210> 18 <211> 948 <212> DNA <213> Artificial Sequence <220> <223> ASFV F317L <400> 18 gttgagacac aaatggacaa acttggtttt ctgctaaatc acataggtaa acaggttacc 60 actaaggtgc ttagcaatgc ccatataact caaacgatga aggagattat tttggaaaat 120 catagtgtag atggtggagc cgcaaaaaat gtttcaaaag ggaagtcttc cccaaaagaa 180 aaaaaacatt ggactgagtt cgaatcctgg gaacagctca gcaagtctaa aagaagtttt 240 aaggaatact gggcggagcg taatgagatt gtgaatactc tgttgcttaa ctgggacaat 300 gttcggggtg ccatcaaaaa atttttggac gatgaccgtg aatggtgcgg ccgcattaat 360 atgataaacg gtgtacccga gatagtggaa atcattccaa gcccctatag ggcaggagag 420 aacatttatt ttggcagcga ggctatgatg cctgctgata tttatagcag ggtggccaac 480 aagcctgcta tgtttgtgtt tcacacgcat cctaatttgg gttcatgttg tggaggaatg 540 ccttccatat gtgacatttc tacaacgctg cgttatctac tcatggggtg gactgctggg 600 catctaatca tttcttcgaa tcaagtagga atgctcacgg ttgataagag aattattgtt 660 gatttgtggg ccaatgagaa tccgcgctgg cttatggcgc aaaaaatatt agatattttt 720 atgatgctca cttcgcgtag aagcctggta aatccctgga ccctgagaga cctaaaaaaa 780 atattacaag actatggtat tgagtatatc atttttcctt cgaatgactt ttttatttat 840 gaagacgaac gtcttttaat gttttcaaaa aaatggacca acttttttac gttacatgag 900 ttattggatg acctcgaaac tattgagaca aaggcatcgt ccacaaca 948 <210> 19 <211> 309 <212> DNA <213> Artificial Sequence <220> <223> ASFV A104R <400> 19 tcgacaaaaa aaaagcccac aattaccaag caagagcttt actccttagt agcggcagat 60 acccagttaa ataaagcatt gattgaaaga atctttacaa gtcagcaaaa aataatacaa 120 aatgctttaa agcacaatca agaagttatt ataccacccg gaatcaagtt caccgtcgtt 180 acggtgaaag ctaaacctgc tcgccagggc cataatcccg ccacaggaga gcctattcaa 240 attaaagcta aacctgaaca taaagccgta aagatacgag cattgaaacc tgtccatgat 300 atgttaaac 309 <210> 20 <211> 612 <212> DNA <213> Artificial Sequence <220> <223> ASFV K205R <400> 20 gttgagccac gcgaacagtt ttttcaagat ctgctttcag cagtggatca acaaatggac 60 actgtaaaaa atgacataaa agacattatg aaagaaaaaa cgtcttttat ggtatcattc 120 gaaaacttta tagaacgtta cgataccatg gaaaaaaata ttcaagacct tcagaataag 180 tacgaagaaa tggcggccaa ccttatgacc gtcatgacgg atacaaaaat tcagcttgga 240 gccattatcg cccaacttga gattctaatg ataaatggca ctccacttcc ggcaaaaaag 300 acaacaatta aggaggctat gcccttacct tcatcaaaca cgaataatga acaaacgagt 360 cctcccgcct caggcaaaac aagtgaaaca cctaaaaaaa atcccacgaa tgcgatgttc 420 ttcacgcgta gcgaatgggc atcctcgaat acttttcgag aaaagttttt aacaccagaa 480 attcaagcca tattggatga gcagtttgca aacaagaccg ggatcgaaag attgcatgcc 540 gagggtcttt acatgtggag aacccaattc tctgacgaac agaagaaaat ggtcaaagag 600 atgatgaaga ag 612 <210> 21 <211> 2855 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 1 <400> 21 Asp Phe Ile Leu Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr 1 5 10 15 Asp Leu Arg Ser Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr 20 25 30 Asn Trp Phe Ser Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr 35 40 45 Ala Ile Lys Thr Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser 50 55 60 Ala Arg Ile Tyr Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu 65 70 75 80 Glu Trp Asn Met Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser 85 90 95 Ser Ala Ser Ser Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn 100 105 110 Glu Cys Thr Ser Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser 115 120 125 Glu Thr Pro Lys Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val 130 135 140 Gln His Ile Glu Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile 145 150 155 160 Arg Ala His Asn Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu 165 170 175 Glu Glu Lys Glu Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys 180 185 190 Lys Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu 195 200 205 Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr 210 215 220 Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile Ile Val Leu 225 230 235 240 Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu 245 250 255 Glu Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala 260 265 270 Glu Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr 275 280 285 Ala Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro 290 295 300 Ala Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val 305 310 315 320 Met Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro 325 330 335 Ala His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala 340 345 350 Ser Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr 355 360 365 Thr His Lys Asp Leu Glu Asn Ser Leu Phe Ser Lys Lys Lys Tyr Ile 370 375 380 Gly Leu Ile Asp Lys Lys Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr 385 390 395 400 Ser Ile Leu Ile Ile Gly Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu 405 410 415 Ile Tyr Asn Phe Ile Gly Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn 420 425 430 Asp Asp Lys Met Phe Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn 435 440 445 Val Cys Tyr Tyr Phe Ser Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser 450 455 460 Asn Tyr Cys Lys Gln Leu Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn 465 470 475 480 Leu Val Asn Leu Thr Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn His 485 490 495 Glu Ser Asn Tyr Trp Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val 500 505 510 Leu Leu Arg Asn Ser Gly Tyr Tyr Lys Lys Gln Lys His Val Ser Leu 515 520 525 Leu Tyr Ile Cys Ser Lys Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn 530 535 540 Ile Val Leu Ser Ile Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile 545 550 555 560 Leu Asp Ser Asn Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly Val Ser 565 570 575 Trp Asn Thr Tyr Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly 580 585 590 Lys Ala Gly Asn Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu Tyr 595 600 605 Asn Ile Thr Asn Asn Cys Ser Leu Thr Ile Phe Pro His Asn Thr Asp 610 615 620 Ile Phe Asn Thr Thr Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile Asn 625 630 635 640 Tyr Thr Ile Lys Leu Leu Thr Pro Val Asp Ser Pro Asn Ile Thr Tyr 645 650 655 Asn Cys Thr Asn Ser Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn 660 665 670 Thr Asn Ile Tyr Leu Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr Asn 675 680 685 Glu Ser Ile Leu Asn Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn Phe 690 695 700 Thr Ala Thr Cys Ile Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu Thr 705 710 715 720 Glu Leu Ile Asn Cys Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr Phe 725 730 735 Tyr Thr Phe Phe Lys Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser Gly 740 745 750 Ile Ile Ile Ser Ile Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser Leu 755 760 765 Arg Lys Arg Lys Lys His Val Glu Glu Ile Glu Ser Pro Pro Pro Ser 770 775 780 Glu Ser Asn Glu Glu Glu Gln Cys Gln His Asp Asp Thr Thr Ser Ile 785 790 795 800 His Glu Pro Ser Pro Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser Arg 805 810 815 Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln Pro 820 825 830 Leu Asn Pro Phe Pro Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys 835 840 845 Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala Glu 850 855 860 Ser Tyr Ser Pro Pro Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro Asn 865 870 875 880 Ile Pro Pro Leu Ser Thr Gln Asn Ile Ser Leu Ile His Val Asp Arg 885 890 895 Ile Ile Tyr His Asp Tyr Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser 900 905 910 Asp Pro Pro Leu Trp Glu Ser Asp Leu Pro Arg His Asn Arg Tyr Ser 915 920 925 Asp Asn Ile Leu Asn Ser Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala 930 935 940 Pro Val Tyr Asn Glu Tyr Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly 945 950 955 960 Leu Gln Leu Ser Asp Leu Arg Asn Phe Ser Phe Met Leu Asn Pro Gln 965 970 975 His Lys Asn Ile Gly Tyr Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser 980 985 990 Ser Ile Pro Lys Asn Lys Leu Phe Asn His Phe Lys Asn His Arg Pro 995 1000 1005 Ala Phe Ser Thr His Thr Glu Asn Leu Ile Arg Arg Asn Val Val Arg 1010 1015 1020 Thr Glu Lys Lys Thr Phe Pro Gln Val Ala Ser Leu Lys Gly Thr Gln 1025 1030 1035 1040 Lys Asn Cys Leu Thr Gln Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro 1045 1050 1055 Lys Asn Ser Ser Val Pro Ser Thr Arg Phe Ser Glu His Thr Lys Phe 1060 1065 1070 Phe Ser Tyr Glu Asp Leu Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys 1075 1080 1085 His Glu Gln His Leu Gly Asp Gln Met Pro Gly Gln His Tyr Asn Gly 1090 1095 1100 Tyr Ile Pro His Lys Asp Val Tyr Asn Ile Leu Cys Leu Ala His Asn 1105 1110 1115 1120 Leu Pro Ala Ser Val Glu Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu 1125 1130 1135 Gly Asn Pro His Val Lys Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser 1140 1145 1150 Thr Ser Thr Tyr Thr Asp Val Pro Met Leu Gly Pro Leu Pro Pro Lys 1155 1160 1165 Asp Ser Gln His Gly Arg Glu Tyr Gln Glu Phe Ser Ala Asn Arg His 1170 1175 1180 Met Leu Gln Val Ser Asn Ile Leu His Ser Val Phe Ala Asn His Ser 1185 1190 1195 1200 Ile Lys Pro Gln Ile Leu Glu Asp Ile Pro Val Leu Asn Ala Gln Leu 1205 1210 1215 Thr Ser Ile Lys Pro Val Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr 1220 1225 1230 His Tyr Met Glu Asn Ile Val Thr Leu Val Pro Arg Phe Lys Ser Ile 1235 1240 1245 Ala Asn Tyr Ser Ser Pro Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly 1250 1255 1260 Gln Ala Glu Tyr Phe Asp Thr Ser Lys Gln Thr Ile Ser Arg His Asn 1265 1270 1275 1280 Asn Tyr Ile Pro Lys Tyr Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp 1285 1290 1295 Ser Thr Phe Pro Lys Asp Phe Asn Ala Ser Ser Val Pro Leu Thr Ser 1300 1305 1310 Ala Glu Lys Asp His Ser Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile 1315 1320 1325 Ser Ser Ile Ser Pro Ser Leu Pro Ser Asn Met Lys Gln Phe Cys Lys 1330 1335 1340 Ile Ser Val Trp Leu Gln Gln His Asp Pro Asp Leu Leu Glu Ile Ile 1345 1350 1355 1360 Asn Asn Leu Cys Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys His 1365 1370 1375 Gly Val Thr Phe Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu Ile 1380 1385 1390 Lys Lys His Ala Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr Leu 1395 1400 1405 Glu Ser Leu Ile Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe Thr 1410 1415 1420 Gly Glu Ile Gly Ser Tyr Thr Gly Val Lys Leu Glu Val Glu Lys Thr 1425 1430 1435 1440 Glu Ala Asn Lys Val Ile Leu Lys Asn Gly Glu Ala Val Leu Val Pro 1445 1450 1455 Ala Ala Asp Phe Lys Pro Phe Pro Asp Arg Arg Leu Ala Val Trp Ile 1460 1465 1470 Met Glu Ser Gly Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg Lys 1475 1480 1485 Lys Glu Gly Gly Gly Asn Asp Pro Pro Val Pro Lys His Ile Ser Pro 1490 1495 1500 Tyr Thr Pro Arg Thr Arg Ile Ala Ile Glu Val Glu Lys Ala Phe Asp 1505 1510 1515 1520 Asp Cys Met Arg Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu Ala 1525 1530 1535 Lys Ser Val Ser Leu Leu Ser Phe Leu Ser Leu Asn His Pro Thr Glu 1540 1545 1550 Phe Ile Lys Val Leu Pro Leu Ile Asp Phe Asp Pro Leu Val Thr Phe 1555 1560 1565 Tyr Leu Leu Leu Glu Pro Tyr Lys Thr His Gly Asp Asp Phe Leu Ile 1570 1575 1580 Pro Glu Thr Ile Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp Leu 1585 1590 1595 1600 Tyr Gln Ser Ala Met Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile Thr 1605 1610 1615 Arg Gln Thr Phe Met Asp Ile Ala Asp Ser Ala Thr Lys Glu Val Asp 1620 1625 1630 Val Pro Ile Cys Tyr Ser Asp Pro Glu Thr Val His Ser Tyr Ala Asn 1635 1640 1645 His Val Arg Thr Glu Ile Leu His His Asn Ala Val Asn Lys Val Thr 1650 1655 1660 Thr Pro Asn Leu Val Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr Asn 1665 1670 1675 1680 Thr Ile Arg His Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn Ala 1685 1690 1695 Leu Arg Phe Trp Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe Val Ile 1700 1705 1710 His Gly Leu His Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr Ser 1715 1720 1725 Glu Phe Ala Glu Ile Val Arg Asn Leu Arg Phe Ser Arg Pro Gly Asn 1730 1735 1740 Asn Tyr Ile Asn Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly Asp 1745 1750 1755 1760 Lys His Thr Thr Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala Met Leu 1765 1770 1775 Val Ala Phe Ile Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro Glu 1780 1785 1790 Glu Lys Val Gly Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp Leu 1795 1800 1805 Val Pro Thr Arg Leu His Ser Phe Leu Asn His Asn Leu Ser Lys Leu 1810 1815 1820 Lys Ile Leu Asn Arg Ala Gln Gln Thr Val Arg Asn Ile Leu Ser Asn 1825 1830 1835 1840 Asp Cys Leu Asn Gln Leu Lys His Tyr Val Lys His Thr Gly Lys Asn 1845 1850 1855 Glu Ile Leu Lys Leu Leu Gln Glu Tyr Phe Leu Val Ala Asp His Arg 1860 1865 1870 Glu His His Val Ile Pro Phe Leu Lys Thr Asp Phe His His Met His 1875 1880 1885 Gln Asn Pro Ile Gln Lys Lys Gln Ala Leu Leu Glu Ile Lys Gln Leu 1890 1895 1900 Phe Thr Gly Asp Tyr Leu Ile Cys Lys Ser Pro Ser Thr Ile Leu Ala 1905 1910 1915 1920 Cys Ile Glu Arg Lys Thr Tyr Lys Asp Phe Ala Ala Ser Leu Lys Asp 1925 1930 1935 Gly Arg Tyr Lys Asn Arg Gln Lys Met Leu Ser Leu Arg Glu Gln Thr 1940 1945 1950 Asn Cys Gln Leu Tyr Phe Phe Val Glu Gly Pro Ala Phe Pro Asn Pro 1955 1960 1965 Gln Lys Lys Ile Asn His Val Ala Tyr Ala Ser Ile Ile Thr Ala Met 1970 1975 1980 Thr His Leu Met Val Arg Asp His Ile Phe Val Ile Gln Thr Lys Asn 1985 1990 1995 2000 Glu Ala His Ser Ser Gln Lys Leu Val Gln Leu Phe Tyr Ala Phe Ser 2005 2010 2015 Lys Glu Met Val Cys Val Val Pro Thr Ser Leu Thr Pro Thr Asp Glu 2020 2025 2030 Glu Leu Cys Ile Lys Leu Trp Ser Ser Leu Ser Gly Ile Ser Gly Val 2035 2040 2045 Ile Gly Lys Ile Leu Ala Asn Thr Cys Ser Val Ala His Leu Val Ser 2050 2055 2060 Gly Lys Leu Ser Ser Gln Asn Ile Asp Gln Leu Lys Thr Pro Ser Asn 2065 2070 2075 2080 Arg Pro Phe Pro Lys Lys Val Lys Arg Met Leu Ile Ser Ile Ser Lys 2085 2090 2095 Gly Asn Lys Glu Leu Glu Ile Lys Leu Leu Ser Gly Val Pro Asn Ile 2100 2105 2110 Gly Lys Lys Leu Ala Ala Glu Ile Leu Lys Asp His Ala Leu Leu Phe 2115 2120 2125 Phe Leu Asn Gln Pro Val Glu Cys Leu Ala Asn Ile Gln Ile Val Gln 2130 2135 2140 Lys Thr Arg Thr Ile Lys Leu Gly Met Lys Arg Ala Glu Ala Ile His 2145 2150 2155 2160 Tyr Phe Leu Asn Trp Cys Gly Ser Ala His Val Thr Asp Asp Ser Gln 2165 2170 2175 Asn Ile Thr Glu Ala Ser Arg Pro Thr Met Gln Val Ala Thr Gln Ser 2180 2185 2190 Ala Ala Thr Gln Pro Ala Ala Thr Gln Pro Leu His Glu Val Ser Asp 2195 2200 2205 Asp Ala Ser Ser Asp Ala Ser Ser Pro Val Gly His Gln Thr Leu Ser 2210 2215 2220 Lys Glu Met Leu Leu Asn Thr Ala Val Glu Thr Gln Met Asp Lys Leu 2225 2230 2235 2240 Gly Phe Leu Leu Asn His Ile Gly Lys Gln Val Thr Thr Lys Val Leu 2245 2250 2255 Ser Asn Ala His Ile Thr Gln Thr Met Lys Glu Ile Ile Leu Glu Asn 2260 2265 2270 His Ser Val Asp Gly Gly Ala Ala Lys Asn Val Ser Lys Gly Lys Ser 2275 2280 2285 Ser Pro Lys Glu Lys Lys His Trp Thr Glu Phe Glu Ser Trp Glu Gln 2290 2295 2300 Leu Ser Lys Ser Lys Arg Ser Phe Lys Glu Tyr Trp Ala Glu Arg Asn 2305 2310 2315 2320 Glu Ile Val Asn Thr Leu Leu Leu Asn Trp Asp Asn Val Arg Gly Ala 2325 2330 2335 Ile Lys Lys Phe Leu Asp Asp Asp Arg Glu Trp Cys Gly Arg Ile Asn 2340 2345 2350 Met Ile Asn Gly Val Pro Glu Ile Val Glu Ile Ile Pro Ser Pro Tyr 2355 2360 2365 Arg Ala Gly Glu Asn Ile Tyr Phe Gly Ser Glu Ala Met Met Pro Ala 2370 2375 2380 Asp Ile Tyr Ser Arg Val Ala Asn Lys Pro Ala Met Phe Val Phe His 2385 2390 2395 2400 Thr His Pro Asn Leu Gly Ser Cys Cys Gly Gly Met Pro Ser Ile Cys 2405 2410 2415 Asp Ile Ser Thr Thr Leu Arg Tyr Leu Leu Met Gly Trp Thr Ala Gly 2420 2425 2430 His Leu Ile Ile Ser Ser Asn Gln Val Gly Met Leu Thr Val Asp Lys 2435 2440 2445 Arg Ile Ile Val Asp Leu Trp Ala Asn Glu Asn Pro Arg Trp Leu Met 2450 2455 2460 Ala Gln Lys Ile Leu Asp Ile Phe Met Met Leu Thr Ser Arg Arg Ser 2465 2470 2475 2480 Leu Val Asn Pro Trp Thr Leu Arg Asp Leu Lys Lys Ile Leu Gln Asp 2485 2490 2495 Tyr Gly Ile Glu Tyr Ile Ile Phe Pro Ser Asn Asp Phe Phe Ile Tyr 2500 2505 2510 Glu Asp Glu Arg Leu Leu Met Phe Ser Lys Lys Trp Thr Asn Phe Phe 2515 2520 2525 Thr Leu His Glu Leu Leu Asp Asp Leu Glu Thr Ile Glu Thr Lys Ala 2530 2535 2540 Ser Ser Thr Thr Ser Thr Lys Lys Lys Pro Thr Ile Thr Lys Gln Glu 2545 2550 2555 2560 Leu Tyr Ser Leu Val Ala Ala Asp Thr Gln Leu Asn Lys Ala Leu Ile 2565 2570 2575 Glu Arg Ile Phe Thr Ser Gln Gln Lys Ile Ile Gln Asn Ala Leu Lys 2580 2585 2590 His Asn Gln Glu Val Ile Ile Pro Pro Gly Ile Lys Phe Thr Val Val 2595 2600 2605 Thr Val Lys Ala Lys Pro Ala Arg Gln Gly His Asn Pro Ala Thr Gly 2610 2615 2620 Glu Pro Ile Gln Ile Lys Ala Lys Pro Glu His Lys Ala Val Lys Ile 2625 2630 2635 2640 Arg Ala Leu Lys Pro Val His Asp Met Leu Asn Val Glu Pro Arg Glu 2645 2650 2655 Gln Phe Phe Gln Asp Leu Leu Ser Ala Val Asp Gln Gln Met Asp Thr 2660 2665 2670 Val Lys Asn Asp Ile Lys Asp Ile Met Lys Glu Lys Thr Ser Phe Met 2675 2680 2685 Val Ser Phe Glu Asn Phe Ile Glu Arg Tyr Asp Thr Met Glu Lys Asn 2690 2695 2700 Ile Gln Asp Leu Gln Asn Lys Tyr Glu Glu Met Ala Ala Asn Leu Met 2705 2710 2715 2720 Thr Val Met Thr Asp Thr Lys Ile Gln Leu Gly Ala Ile Ile Ala Gln 2725 2730 2735 Leu Glu Ile Leu Met Ile Asn Gly Thr Pro Leu Pro Ala Lys Lys Thr 2740 2745 2750 Thr Ile Lys Glu Ala Met Pro Leu Pro Ser Ser Asn Thr Asn Asn Glu 2755 2760 2765 Gln Thr Ser Pro Pro Ala Ser Gly Lys Thr Ser Glu Thr Pro Lys Lys 2770 2775 2780 Asn Pro Thr Asn Ala Met Phe Phe Thr Arg Ser Glu Trp Ala Ser Ser 2785 2790 2795 2800 Asn Thr Phe Arg Glu Lys Phe Leu Thr Pro Glu Ile Gln Ala Ile Leu 2805 2810 2815 Asp Glu Gln Phe Ala Asn Lys Thr Gly Ile Glu Arg Leu His Ala Glu 2820 2825 2830 Gly Leu Tyr Met Trp Arg Thr Gln Phe Ser Asp Glu Gln Lys Lys Met 2835 2840 2845 Val Lys Glu Met Met Lys Lys 2850 2855 <210> 22 <211> 898 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 2 <400> 22 Asp Phe Ile Leu Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr 1 5 10 15 Asp Leu Arg Ser Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr 20 25 30 Asn Trp Phe Ser Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr 35 40 45 Ala Ile Lys Thr Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser 50 55 60 Ala Arg Ile Tyr Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu 65 70 75 80 Glu Trp Asn Met Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser 85 90 95 Ser Ala Ser Ser Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn 100 105 110 Glu Cys Thr Ser Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser 115 120 125 Glu Thr Pro Lys Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val 130 135 140 Gln His Ile Glu Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile 145 150 155 160 Arg Ala His Asn Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu 165 170 175 Glu Glu Lys Glu Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys 180 185 190 Lys Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu 195 200 205 Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr 210 215 220 Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile Ile Val Leu 225 230 235 240 Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu 245 250 255 Glu Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala 260 265 270 Glu Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr 275 280 285 Ala Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro 290 295 300 Ala Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val 305 310 315 320 Met Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro 325 330 335 Ala His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala 340 345 350 Ser Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr 355 360 365 Thr His Lys Asp Leu Glu Asn Ser Leu Phe Ser Lys Lys Lys Tyr Ile 370 375 380 Gly Leu Ile Asp Lys Lys Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr 385 390 395 400 Ser Ile Leu Ile Ile Gly Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu 405 410 415 Ile Tyr Asn Phe Ile Gly Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn 420 425 430 Asp Asp Lys Met Phe Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn 435 440 445 Val Cys Tyr Tyr Phe Ser Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser 450 455 460 Asn Tyr Cys Lys Gln Leu Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn 465 470 475 480 Leu Val Asn Leu Thr Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn His 485 490 495 Glu Ser Asn Tyr Trp Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val 500 505 510 Leu Leu Arg Asn Ser Gly Tyr Tyr Lys Lys Gln Lys His Val Ser Leu 515 520 525 Leu Tyr Ile Cys Ser Lys Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn 530 535 540 Ile Val Leu Ser Ile Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile 545 550 555 560 Leu Asp Ser Asn Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly Val Ser 565 570 575 Trp Asn Thr Tyr Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly 580 585 590 Lys Ala Gly Asn Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu Tyr 595 600 605 Asn Ile Thr Asn Asn Cys Ser Leu Thr Ile Phe Pro His Asn Thr Asp 610 615 620 Ile Phe Asn Thr Thr Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile Asn 625 630 635 640 Tyr Thr Ile Lys Leu Leu Thr Pro Val Asp Ser Pro Asn Ile Thr Tyr 645 650 655 Asn Cys Thr Asn Ser Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn 660 665 670 Thr Asn Ile Tyr Leu Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr Asn 675 680 685 Glu Ser Ile Leu Asn Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn Phe 690 695 700 Thr Ala Thr Cys Ile Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu Thr 705 710 715 720 Glu Leu Ile Asn Cys Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr Phe 725 730 735 Tyr Thr Phe Phe Lys Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser Gly 740 745 750 Ile Ile Ile Ser Ile Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser Leu 755 760 765 Arg Lys Arg Lys Lys His Val Glu Glu Ile Glu Ser Pro Pro Pro Ser 770 775 780 Glu Ser Asn Glu Glu Glu Gln Cys Gln His Asp Asp Thr Thr Ser Ile 785 790 795 800 His Glu Pro Ser Pro Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser Arg 805 810 815 Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln Pro 820 825 830 Leu Asn Pro Phe Pro Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys 835 840 845 Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala Glu 850 855 860 Ser Tyr Ser Pro Pro Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro Asn 865 870 875 880 Ile Pro Pro Leu Ser Thr Gln Asn Ile Ser Leu Ile His Val Asp Arg 885 890 895 Ile Ile <210> 23 <211> 1957 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 3 <400> 23 Tyr His Asp Tyr Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro 1 5 10 15 Pro Leu Trp Glu Ser Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn 20 25 30 Ile Leu Asn Ser Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val 35 40 45 Tyr Asn Glu Tyr Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln 50 55 60 Leu Ser Asp Leu Arg Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys 65 70 75 80 Asn Ile Gly Tyr Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile 85 90 95 Pro Lys Asn Lys Leu Phe Asn His Phe Lys Asn His Arg Pro Ala Phe 100 105 110 Ser Thr His Thr Glu Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu 115 120 125 Lys Lys Thr Phe Pro Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn 130 135 140 Cys Leu Thr Gln Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn 145 150 155 160 Ser Ser Val Pro Ser Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser 165 170 175 Tyr Glu Asp Leu Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu 180 185 190 Gln His Leu Gly Asp Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile 195 200 205 Pro His Lys Asp Val Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro 210 215 220 Ala Ser Val Glu Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn 225 230 235 240 Pro His Val Lys Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser 245 250 255 Thr Tyr Thr Asp Val Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser 260 265 270 Gln His Gly Arg Glu Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu 275 280 285 Gln Val Ser Asn Ile Leu His Ser Val Phe Ala Asn His Ser Ile Lys 290 295 300 Pro Gln Ile Leu Glu Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser 305 310 315 320 Ile Lys Pro Val Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr 325 330 335 Met Glu Asn Ile Val Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn 340 345 350 Tyr Ser Ser Pro Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala 355 360 365 Glu Tyr Phe Asp Thr Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr 370 375 380 Ile Pro Lys Tyr Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr 385 390 395 400 Phe Pro Lys Asp Phe Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu 405 410 415 Lys Asp His Ser Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser 420 425 430 Ile Ser Pro Ser Leu Pro Ser Asn Met Lys Gln Phe Cys Lys Ile Ser 435 440 445 Val Trp Leu Gln Gln His Asp Pro Asp Leu Leu Glu Ile Ile Asn Asn 450 455 460 Leu Cys Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys His Gly Val 465 470 475 480 Thr Phe Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys Lys 485 490 495 His Ala Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu Ser 500 505 510 Leu Ile Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly Glu 515 520 525 Ile Gly Ser Tyr Thr Gly Val Lys Leu Glu Val Glu Lys Thr Glu Ala 530 535 540 Asn Lys Val Ile Leu Lys Asn Gly Glu Ala Val Leu Val Pro Ala Ala 545 550 555 560 Asp Phe Lys Pro Phe Pro Asp Arg Arg Leu Ala Val Trp Ile Met Glu 565 570 575 Ser Gly Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys Glu 580 585 590 Gly Gly Gly Asn Asp Pro Pro Val Pro Lys His Ile Ser Pro Tyr Thr 595 600 605 Pro Arg Thr Arg Ile Ala Ile Glu Val Glu Lys Ala Phe Asp Asp Cys 610 615 620 Met Arg Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys Ser 625 630 635 640 Val Ser Leu Leu Ser Phe Leu Ser Leu Asn His Pro Thr Glu Phe Ile 645 650 655 Lys Val Leu Pro Leu Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr Leu 660 665 670 Leu Leu Glu Pro Tyr Lys Thr His Gly Asp Asp Phe Leu Ile Pro Glu 675 680 685 Thr Ile Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr Gln 690 695 700 Ser Ala Met Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg Gln 705 710 715 720 Thr Phe Met Asp Ile Ala Asp Ser Ala Thr Lys Glu Val Asp Val Pro 725 730 735 Ile Cys Tyr Ser Asp Pro Glu Thr Val His Ser Tyr Ala Asn His Val 740 745 750 Arg Thr Glu Ile Leu His His Asn Ala Val Asn Lys Val Thr Thr Pro 755 760 765 Asn Leu Val Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr Ile 770 775 780 Arg His Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu Arg 785 790 795 800 Phe Trp Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe Val Ile His Gly 805 810 815 Leu His Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu Phe 820 825 830 Ala Glu Ile Val Arg Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn Tyr 835 840 845 Ile Asn Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys His 850 855 860 Thr Thr Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala Met Leu Val Ala 865 870 875 880 Phe Ile Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu Lys 885 890 895 Val Gly Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp Leu Val Pro 900 905 910 Thr Arg Leu His Ser Phe Leu Asn His Asn Leu Ser Lys Leu Lys Ile 915 920 925 Leu Asn Arg Ala Gln Gln Thr Val Arg Asn Ile Leu Ser Asn Asp Cys 930 935 940 Leu Asn Gln Leu Lys His Tyr Val Lys His Thr Gly Lys Asn Glu Ile 945 950 955 960 Leu Lys Leu Leu Gln Glu Tyr Phe Leu Val Ala Asp His Arg Glu His 965 970 975 His Val Ile Pro Phe Leu Lys Thr Asp Phe His His Met His Gln Asn 980 985 990 Pro Ile Gln Lys Lys Gln Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr 995 1000 1005 Gly Asp Tyr Leu Ile Cys Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile 1010 1015 1020 Glu Arg Lys Thr Tyr Lys Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg 1025 1030 1035 1040 Tyr Lys Asn Arg Gln Lys Met Leu Ser Leu Arg Glu Gln Thr Asn Cys 1045 1050 1055 Gln Leu Tyr Phe Phe Val Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys 1060 1065 1070 Lys Ile Asn His Val Ala Tyr Ala Ser Ile Ile Thr Ala Met Thr His 1075 1080 1085 Leu Met Val Arg Asp His Ile Phe Val Ile Gln Thr Lys Asn Glu Ala 1090 1095 1100 His Ser Ser Gln Lys Leu Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu 1105 1110 1115 1120 Met Val Cys Val Val Pro Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu 1125 1130 1135 Cys Ile Lys Leu Trp Ser Ser Leu Ser Gly Ile Ser Gly Val Ile Gly 1140 1145 1150 Lys Ile Leu Ala Asn Thr Cys Ser Val Ala His Leu Val Ser Gly Lys 1155 1160 1165 Leu Ser Ser Gln Asn Ile Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro 1170 1175 1180 Phe Pro Lys Lys Val Lys Arg Met Leu Ile Ser Ile Ser Lys Gly Asn 1185 1190 1195 1200 Lys Glu Leu Glu Ile Lys Leu Leu Ser Gly Val Pro Asn Ile Gly Lys 1205 1210 1215 Lys Leu Ala Ala Glu Ile Leu Lys Asp His Ala Leu Leu Phe Phe Leu 1220 1225 1230 Asn Gln Pro Val Glu Cys Leu Ala Asn Ile Gln Ile Val Gln Lys Thr 1235 1240 1245 Arg Thr Ile Lys Leu Gly Met Lys Arg Ala Glu Ala Ile His Tyr Phe 1250 1255 1260 Leu Asn Trp Cys Gly Ser Ala His Val Thr Asp Asp Ser Gln Asn Ile 1265 1270 1275 1280 Thr Glu Ala Ser Arg Pro Thr Met Gln Val Ala Thr Gln Ser Ala Ala 1285 1290 1295 Thr Gln Pro Ala Ala Thr Gln Pro Leu His Glu Val Ser Asp Asp Ala 1300 1305 1310 Ser Ser Asp Ala Ser Ser Pro Val Gly His Gln Thr Leu Ser Lys Glu 1315 1320 1325 Met Leu Leu Asn Thr Ala Val Glu Thr Gln Met Asp Lys Leu Gly Phe 1330 1335 1340 Leu Leu Asn His Ile Gly Lys Gln Val Thr Thr Lys Val Leu Ser Asn 1345 1350 1355 1360 Ala His Ile Thr Gln Thr Met Lys Glu Ile Ile Leu Glu Asn His Ser 1365 1370 1375 Val Asp Gly Gly Ala Ala Lys Asn Val Ser Lys Gly Lys Ser Ser Pro 1380 1385 1390 Lys Glu Lys Lys His Trp Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser 1395 1400 1405 Lys Ser Lys Arg Ser Phe Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile 1410 1415 1420 Val Asn Thr Leu Leu Leu Asn Trp Asp Asn Val Arg Gly Ala Ile Lys 1425 1430 1435 1440 Lys Phe Leu Asp Asp Asp Arg Glu Trp Cys Gly Arg Ile Asn Met Ile 1445 1450 1455 Asn Gly Val Pro Glu Ile Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala 1460 1465 1470 Gly Glu Asn Ile Tyr Phe Gly Ser Glu Ala Met Met Pro Ala Asp Ile 1475 1480 1485 Tyr Ser Arg Val Ala Asn Lys Pro Ala Met Phe Val Phe His Thr His 1490 1495 1500 Pro Asn Leu Gly Ser Cys Cys Gly Gly Met Pro Ser Ile Cys Asp Ile 1505 1510 1515 1520 Ser Thr Thr Leu Arg Tyr Leu Leu Met Gly Trp Thr Ala Gly His Leu 1525 1530 1535 Ile Ile Ser Ser Asn Gln Val Gly Met Leu Thr Val Asp Lys Arg Ile 1540 1545 1550 Ile Val Asp Leu Trp Ala Asn Glu Asn Pro Arg Trp Leu Met Ala Gln 1555 1560 1565 Lys Ile Leu Asp Ile Phe Met Met Leu Thr Ser Arg Arg Ser Leu Val 1570 1575 1580 Asn Pro Trp Thr Leu Arg Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly 1585 1590 1595 1600 Ile Glu Tyr Ile Ile Phe Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp 1605 1610 1615 Glu Arg Leu Leu Met Phe Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu 1620 1625 1630 His Glu Leu Leu Asp Asp Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser 1635 1640 1645 Thr Thr Ser Thr Lys Lys Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr 1650 1655 1660 Ser Leu Val Ala Ala Asp Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg 1665 1670 1675 1680 Ile Phe Thr Ser Gln Gln Lys Ile Ile Gln Asn Ala Leu Lys His Asn 1685 1690 1695 Gln Glu Val Ile Ile Pro Pro Gly Ile Lys Phe Thr Val Val Thr Val 1700 1705 1710 Lys Ala Lys Pro Ala Arg Gln Gly His Asn Pro Ala Thr Gly Glu Pro 1715 1720 1725 Ile Gln Ile Lys Ala Lys Pro Glu His Lys Ala Val Lys Ile Arg Ala 1730 1735 1740 Leu Lys Pro Val His Asp Met Leu Asn Val Glu Pro Arg Glu Gln Phe 1745 1750 1755 1760 Phe Gln Asp Leu Leu Ser Ala Val Asp Gln Gln Met Asp Thr Val Lys 1765 1770 1775 Asn Asp Ile Lys Asp Ile Met Lys Glu Lys Thr Ser Phe Met Val Ser 1780 1785 1790 Phe Glu Asn Phe Ile Glu Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln 1795 1800 1805 Asp Leu Gln Asn Lys Tyr Glu Glu Met Ala Ala Asn Leu Met Thr Val 1810 1815 1820 Met Thr Asp Thr Lys Ile Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu 1825 1830 1835 1840 Ile Leu Met Ile Asn Gly Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile 1845 1850 1855 Lys Glu Ala Met Pro Leu Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr 1860 1865 1870 Ser Pro Pro Ala Ser Gly Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro 1875 1880 1885 Thr Asn Ala Met Phe Phe Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr 1890 1895 1900 Phe Arg Glu Lys Phe Leu Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu 1905 1910 1915 1920 Gln Phe Ala Asn Lys Thr Gly Ile Glu Arg Leu His Ala Glu Gly Leu 1925 1930 1935 Tyr Met Trp Arg Thr Gln Phe Ser Asp Glu Gln Lys Lys Met Val Lys 1940 1945 1950 Glu Met Met Lys Lys 1955 <210> 24 <211> 1427 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 4 <400> 24 Asp Phe Ile Leu Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr 1 5 10 15 Asp Leu Arg Ser Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr 20 25 30 Asn Trp Phe Ser Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr 35 40 45 Ala Ile Lys Thr Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser 50 55 60 Ala Arg Ile Tyr Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu 65 70 75 80 Glu Trp Asn Met Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser 85 90 95 Ser Ala Ser Ser Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn 100 105 110 Glu Cys Thr Ser Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser 115 120 125 Glu Thr Pro Lys Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val 130 135 140 Gln His Ile Glu Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile 145 150 155 160 Arg Ala His Asn Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu 165 170 175 Glu Glu Lys Glu Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys 180 185 190 Lys Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu 195 200 205 Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr 210 215 220 Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile Ile Val Leu 225 230 235 240 Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu 245 250 255 Glu Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala 260 265 270 Glu Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr 275 280 285 Ala Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro 290 295 300 Ala Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val 305 310 315 320 Met Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro 325 330 335 Ala His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala 340 345 350 Ser Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr 355 360 365 Thr His Lys Asp Leu Glu Asn Ser Leu Pro Ser Asn Met Lys Gln Phe 370 375 380 Cys Lys Ile Ser Val Trp Leu Gln Gln His Asp Pro Asp Leu Leu Glu 385 390 395 400 Ile Ile Asn Asn Leu Cys Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr 405 410 415 Lys His Gly Val Thr Phe Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp 420 425 430 Glu Ile Lys Lys His Ala Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys 435 440 445 Thr Leu Glu Ser Leu Ile Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu 450 455 460 Phe Thr Gly Glu Ile Gly Ser Tyr Thr Gly Val Lys Leu Glu Val Glu 465 470 475 480 Lys Thr Glu Ala Asn Lys Val Ile Leu Lys Asn Gly Glu Ala Val Leu 485 490 495 Val Pro Ala Ala Asp Phe Lys Pro Phe Pro Asp Arg Arg Leu Ala Val 500 505 510 Trp Ile Met Glu Ser Gly Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys 515 520 525 Arg Lys Lys Glu Gly Gly Gly Asn Asp Pro Pro Val Pro Lys His Ile 530 535 540 Ser Pro Tyr Thr Pro Arg Thr Arg Ile Ala Ile Glu Val Glu Lys Ala 545 550 555 560 Phe Asp Asp Cys Met Arg Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr 565 570 575 Leu Ala Lys Ser Val Ser Leu Leu Ser Phe Leu Ser Leu Asn His Pro 580 585 590 Thr Glu Phe Ile Lys Val Leu Pro Leu Ile Asp Phe Asp Pro Leu Val 595 600 605 Thr Phe Tyr Leu Leu Leu Glu Pro Tyr Lys Thr His Gly Asp Asp Phe 610 615 620 Leu Ile Pro Glu Thr Ile Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr 625 630 635 640 Asp Leu Tyr Gln Ser Ala Met Leu Glu Phe Lys Lys Phe Phe Thr Gln 645 650 655 Ile Thr Arg Gln Thr Phe Met Asp Ile Ala Asp Ser Ala Thr Lys Glu 660 665 670 Val Asp Val Pro Ile Cys Tyr Ser Asp Pro Glu Thr Val His Ser Tyr 675 680 685 Ala Asn His Val Arg Thr Glu Ile Leu His His Asn Ala Val Asn Lys 690 695 700 Val Thr Thr Pro Asn Leu Val Val Gln Ala Tyr Asn Glu Leu Glu Gln 705 710 715 720 Thr Asn Thr Ile Arg His Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile 725 730 735 Asn Ala Leu Arg Phe Trp Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe 740 745 750 Val Ile His Gly Leu His Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu 755 760 765 Thr Ser Glu Phe Ala Glu Ile Val Arg Asn Leu Arg Phe Ser Arg Pro 770 775 780 Gly Asn Asn Tyr Ile Asn Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr 785 790 795 800 Gly Asp Lys His Thr Thr Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala 805 810 815 Met Leu Val Ala Phe Ile Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile 820 825 830 Pro Glu Glu Lys Val Gly Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr 835 840 845 Asp Leu Val Pro Thr Arg Leu His Ser Phe Leu Asn His Asn Leu Ser 850 855 860 Lys Leu Lys Ile Leu Asn Arg Ala Gln Gln Thr Val Arg Asn Ile Leu 865 870 875 880 Ser Asn Asp Cys Leu Asn Gln Leu Lys His Tyr Val Lys His Thr Gly 885 890 895 Lys Asn Glu Ile Leu Lys Leu Leu Gln Glu Phe Ser Lys Lys Lys Tyr 900 905 910 Ile Gly Leu Ile Asp Lys Lys Cys Glu Leu Lys Lys Ile Leu Asp Asp 915 920 925 Tyr Ser Ile Leu Ile Ile Gly Ile Leu Ile Gly Thr Asn Ile Ile Thr 930 935 940 Leu Ile Tyr Asn Phe Ile Gly Trp Ile Asn Tyr Ile Lys Cys Tyr Gln 945 950 955 960 Asn Asp Asp Lys Met Phe Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn 965 970 975 Asn Val Cys Tyr Tyr Phe Ser Asn Glu Glu Lys Asn Tyr Thr Asn Ala 980 985 990 Ser Asn Tyr Cys Lys Gln Leu Asn Ser Thr Leu Ala Asn Asn Asn Thr 995 1000 1005 Asn Leu Val Asn Leu Thr Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn 1010 1015 1020 His Glu Ser Asn Tyr Trp Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser 1025 1030 1035 1040 Val Leu Leu Arg Asn Ser Gly Tyr Tyr Lys Lys Gln Lys His Val Ser 1045 1050 1055 Leu Leu Tyr Ile Cys Ser Lys Ile Ile Leu Ile Phe Leu Ile Phe Ser 1060 1065 1070 Asn Ile Val Leu Ser Ile Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile 1075 1080 1085 Ile Leu Asp Ser Asn Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly Val 1090 1095 1100 Ser Trp Asn Thr Tyr Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys 1105 1110 1115 1120 Gly Lys Ala Gly Asn Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu 1125 1130 1135 Tyr Asn Ile Thr Asn Asn Cys Ser Leu Thr Ile Phe Pro His Asn Thr 1140 1145 1150 Asp Ile Phe Asn Thr Thr Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile 1155 1160 1165 Asn Tyr Thr Ile Lys Leu Leu Thr Pro Val Asp Ser Pro Asn Ile Thr 1170 1175 1180 Tyr Asn Cys Thr Asn Ser Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr 1185 1190 1195 1200 Asn Thr Asn Ile Tyr Leu Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr 1205 1210 1215 Asn Glu Ser Ile Leu Asn Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn 1220 1225 1230 Phe Thr Ala Thr Cys Ile Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu 1235 1240 1245 Thr Glu Leu Ile Asn Cys Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr 1250 1255 1260 Phe Tyr Thr Phe Phe Lys Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser 1265 1270 1275 1280 Gly Ile Ile Ile Ser Ile Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser 1285 1290 1295 Leu Arg Lys Arg Lys Lys His Val Glu Glu Ile Glu Ser Pro Pro Pro 1300 1305 1310 Ser Glu Ser Asn Glu Glu Glu Gln Cys Gln His Asp Asp Thr Thr Ser 1315 1320 1325 Ile His Glu Pro Ser Pro Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser 1330 1335 1340 Arg Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln 1345 1350 1355 1360 Pro Leu Asn Pro Phe Pro Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro 1365 1370 1375 Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala 1380 1385 1390 Glu Ser Tyr Ser Pro Pro Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro 1395 1400 1405 Asn Ile Pro Pro Leu Ser Thr Gln Asn Ile Ser Leu Ile His Val Asp 1410 1415 1420 Arg Ile Ile 1425 <210> 25 <211> 1428 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 5 <400> 25 Tyr His Asp Tyr Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro 1 5 10 15 Pro Leu Trp Glu Ser Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn 20 25 30 Ile Leu Asn Ser Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val 35 40 45 Tyr Asn Glu Tyr Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln 50 55 60 Leu Ser Asp Leu Arg Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys 65 70 75 80 Asn Ile Gly Tyr Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile 85 90 95 Pro Lys Asn Lys Leu Phe Asn His Phe Lys Asn His Arg Pro Ala Phe 100 105 110 Ser Thr His Thr Glu Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu 115 120 125 Lys Lys Thr Phe Pro Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn 130 135 140 Cys Leu Thr Gln Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn 145 150 155 160 Ser Ser Val Pro Ser Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser 165 170 175 Tyr Glu Asp Leu Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu 180 185 190 Gln His Leu Gly Asp Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile 195 200 205 Pro His Lys Asp Val Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro 210 215 220 Ala Ser Val Glu Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn 225 230 235 240 Pro His Val Lys Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser 245 250 255 Thr Tyr Thr Asp Val Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser 260 265 270 Gln His Gly Arg Glu Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu 275 280 285 Gln Val Ser Asn Ile Leu His Ser Val Phe Ala Asn His Ser Ile Lys 290 295 300 Pro Gln Ile Leu Glu Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser 305 310 315 320 Ile Lys Pro Val Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr 325 330 335 Met Glu Asn Ile Val Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn 340 345 350 Tyr Ser Ser Pro Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala 355 360 365 Glu Tyr Phe Asp Thr Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr 370 375 380 Ile Pro Lys Tyr Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr 385 390 395 400 Phe Pro Lys Asp Phe Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu 405 410 415 Lys Asp His Ser Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser 420 425 430 Ile Ser Pro Ser Leu Tyr Phe Leu Val Ala Asp His Arg Glu His His 435 440 445 Val Ile Pro Phe Leu Lys Thr Asp Phe His His Met His Gln Asn Pro 450 455 460 Ile Gln Lys Lys Gln Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr Gly 465 470 475 480 Asp Tyr Leu Ile Cys Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile Glu 485 490 495 Arg Lys Thr Tyr Lys Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg Tyr 500 505 510 Lys Asn Arg Gln Lys Met Leu Ser Leu Arg Glu Gln Thr Asn Cys Gln 515 520 525 Leu Tyr Phe Phe Val Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys Lys 530 535 540 Ile Asn His Val Ala Tyr Ala Ser Ile Ile Thr Ala Met Thr His Leu 545 550 555 560 Met Val Arg Asp His Ile Phe Val Ile Gln Thr Lys Asn Glu Ala His 565 570 575 Ser Ser Gln Lys Leu Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu Met 580 585 590 Val Cys Val Val Pro Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu Cys 595 600 605 Ile Lys Leu Trp Ser Ser Leu Ser Gly Ile Ser Gly Val Ile Gly Lys 610 615 620 Ile Leu Ala Asn Thr Cys Ser Val Ala His Leu Val Ser Gly Lys Leu 625 630 635 640 Ser Ser Gln Asn Ile Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro Phe 645 650 655 Pro Lys Lys Val Lys Arg Met Leu Ile Ser Ile Ser Lys Gly Asn Lys 660 665 670 Glu Leu Glu Ile Lys Leu Leu Ser Gly Val Pro Asn Ile Gly Lys Lys 675 680 685 Leu Ala Ala Glu Ile Leu Lys Asp His Ala Leu Leu Phe Phe Leu Asn 690 695 700 Gln Pro Val Glu Cys Leu Ala Asn Ile Gln Ile Val Gln Lys Thr Arg 705 710 715 720 Thr Ile Lys Leu Gly Met Lys Arg Ala Glu Ala Ile His Tyr Phe Leu 725 730 735 Asn Trp Cys Gly Ser Ala His Val Thr Asp Asp Ser Gln Asn Ile Thr 740 745 750 Glu Ala Ser Arg Pro Thr Met Gln Val Ala Thr Gln Ser Ala Ala Thr 755 760 765 Gln Pro Ala Ala Thr Gln Pro Leu His Glu Val Ser Asp Asp Ala Ser 770 775 780 Ser Asp Ala Ser Ser Pro Val Gly His Gln Thr Leu Ser Lys Glu Met 785 790 795 800 Leu Leu Asn Thr Ala Val Glu Thr Gln Met Asp Lys Leu Gly Phe Leu 805 810 815 Leu Asn His Ile Gly Lys Gln Val Thr Thr Lys Val Leu Ser Asn Ala 820 825 830 His Ile Thr Gln Thr Met Lys Glu Ile Ile Leu Glu Asn His Ser Val 835 840 845 Asp Gly Gly Ala Ala Lys Asn Val Ser Lys Gly Lys Ser Ser Pro Lys 850 855 860 Glu Lys Lys His Trp Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys 865 870 875 880 Ser Lys Arg Ser Phe Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val 885 890 895 Asn Thr Leu Leu Leu Asn Trp Asp Asn Val Arg Gly Ala Ile Lys Lys 900 905 910 Phe Leu Asp Asp Asp Arg Glu Trp Cys Gly Arg Ile Asn Met Ile Asn 915 920 925 Gly Val Pro Glu Ile Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly 930 935 940 Glu Asn Ile Tyr Phe Gly Ser Glu Ala Met Met Pro Ala Asp Ile Tyr 945 950 955 960 Ser Arg Val Ala Asn Lys Pro Ala Met Phe Val Phe His Thr His Pro 965 970 975 Asn Leu Gly Ser Cys Cys Gly Gly Met Pro Ser Ile Cys Asp Ile Ser 980 985 990 Thr Thr Leu Arg Tyr Leu Leu Met Gly Trp Thr Ala Gly His Leu Ile 995 1000 1005 Ile Ser Ser Asn Gln Val Gly Met Leu Thr Val Asp Lys Arg Ile Ile 1010 1015 1020 Val Asp Leu Trp Ala Asn Glu Asn Pro Arg Trp Leu Met Ala Gln Lys 1025 1030 1035 1040 Ile Leu Asp Ile Phe Met Met Leu Thr Ser Arg Arg Ser Leu Val Asn 1045 1050 1055 Pro Trp Thr Leu Arg Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile 1060 1065 1070 Glu Tyr Ile Ile Phe Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu 1075 1080 1085 Arg Leu Leu Met Phe Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu His 1090 1095 1100 Glu Leu Leu Asp Asp Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr 1105 1110 1115 1120 Thr Ser Thr Lys Lys Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr Ser 1125 1130 1135 Leu Val Ala Ala Asp Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg Ile 1140 1145 1150 Phe Thr Ser Gln Gln Lys Ile Ile Gln Asn Ala Leu Lys His Asn Gln 1155 1160 1165 Glu Val Ile Ile Pro Pro Gly Ile Lys Phe Thr Val Val Thr Val Lys 1170 1175 1180 Ala Lys Pro Ala Arg Gln Gly His Asn Pro Ala Thr Gly Glu Pro Ile 1185 1190 1195 1200 Gln Ile Lys Ala Lys Pro Glu His Lys Ala Val Lys Ile Arg Ala Leu 1205 1210 1215 Lys Pro Val His Asp Met Leu Asn Val Glu Pro Arg Glu Gln Phe Phe 1220 1225 1230 Gln Asp Leu Leu Ser Ala Val Asp Gln Gln Met Asp Thr Val Lys Asn 1235 1240 1245 Asp Ile Lys Asp Ile Met Lys Glu Lys Thr Ser Phe Met Val Ser Phe 1250 1255 1260 Glu Asn Phe Ile Glu Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln Asp 1265 1270 1275 1280 Leu Gln Asn Lys Tyr Glu Glu Met Ala Ala Asn Leu Met Thr Val Met 1285 1290 1295 Thr Asp Thr Lys Ile Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu Ile 1300 1305 1310 Leu Met Ile Asn Gly Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile Lys 1315 1320 1325 Glu Ala Met Pro Leu Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr Ser 1330 1335 1340 Pro Pro Ala Ser Gly Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro Thr 1345 1350 1355 1360 Asn Ala Met Phe Phe Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr Phe 1365 1370 1375 Arg Glu Lys Phe Leu Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu Gln 1380 1385 1390 Phe Ala Asn Lys Thr Gly Ile Glu Arg Leu His Ala Glu Gly Leu Tyr 1395 1400 1405 Met Trp Arg Thr Gln Phe Ser Asp Glu Gln Lys Lys Met Val Lys Glu 1410 1415 1420 Met Met Lys Lys 1425 <210> 26 <211> 2930 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 1' <400> 26 Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 1 5 10 15 Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu Lys 20 25 30 Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 35 40 45 Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 50 55 60 Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Asp Phe Ile Leu Asn 65 70 75 80 Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser Ser 85 90 95 Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser Val 100 105 110 Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr Leu 115 120 125 Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr Ala 130 135 140 Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met Ile 145 150 155 160 Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser Glu 165 170 175 Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser Ser 180 185 190 Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Thr Pro Lys Asp 195 200 205 Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu Gln 210 215 220 Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn Phe 225 230 235 240 Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu Val 245 250 255 Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys Lys Asp Ser Glu Phe 260 265 270 Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu Cys Leu Ser Pro Val 275 280 285 Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr Thr Ile Leu Ile Ala 290 295 300 Ile Val Val Leu Val Ile Ile Ile Ile Val Leu Ile Tyr Leu Phe Ser 305 310 315 320 Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu Glu Glu Asp Ile Gln 325 330 335 Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala Glu Val Thr Pro Gln 340 345 350 Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr Ala Ser Val Gly Lys 355 360 365 Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro Ala Thr Asn Lys Pro 370 375 380 Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val Met Ala Thr Gly Gly 385 390 395 400 Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro Ala His Pro Ala Glu 405 410 415 Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala Ser Gln Thr Met Ser 420 425 430 Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr Thr His Lys Asp Leu 435 440 445 Glu Asn Ser Leu Phe Ser Lys Lys Lys Tyr Ile Gly Leu Ile Asp Lys 450 455 460 Lys Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr Ser Ile Leu Ile Ile 465 470 475 480 Gly Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu Ile Tyr Asn Phe Ile 485 490 495 Gly Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn Asp Asp Lys Met Phe 500 505 510 Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr Phe 515 520 525 Ser Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser Asn Tyr Cys Lys Gln 530 535 540 Leu Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn Leu Val Asn Leu Thr 545 550 555 560 Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr Trp 565 570 575 Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asn Ser 580 585 590 Gly Tyr Tyr Lys Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys Ser 595 600 605 Lys Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn Ile Val Leu Ser Ile 610 615 620 Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile Leu Asp Ser Asn Ile 625 630 635 640 Thr Asn Asp Asn Asn Asp Ile Asn Gly Val Ser Trp Asn Thr Tyr Phe 645 650 655 Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly Lys Ala Gly Asn Phe 660 665 670 Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu Tyr Asn Ile Thr Asn Asn 675 680 685 Cys Ser Leu Thr Ile Phe Pro His Asn Thr Asp Ile Phe Asn Thr Thr 690 695 700 Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile Asn Tyr Thr Ile Lys Leu 705 710 715 720 Leu Thr Pro Val Asp Ser Pro Asn Ile Thr Tyr Asn Cys Thr Asn Ser 725 730 735 Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn Thr Asn Ile Tyr Leu 740 745 750 Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr Asn Glu Ser Ile Leu Asn 755 760 765 Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn Phe Thr Ala Thr Cys Ile 770 775 780 Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu Thr Glu Leu Ile Asn Cys 785 790 795 800 Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr Phe Tyr Thr Phe Phe Lys 805 810 815 Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser Gly Ile Ile Ile Ser Ile 820 825 830 Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser Leu Arg Lys Arg Lys Lys 835 840 845 His Val Glu Glu Ile Glu Ser Pro Pro Pro Ser Glu Ser Asn Glu Glu 850 855 860 Glu Gln Cys Gln His Asp Asp Thr Thr Ser Ile His Glu Pro Ser Pro 865 870 875 880 Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn Thr 885 890 895 Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe Pro 900 905 910 Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro 915 920 925 Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro Pro 930 935 940 Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu Ser 945 950 955 960 Thr Gln Asn Ile Ser Leu Ile His Val Asp Arg Ile Ile Tyr His Asp 965 970 975 Tyr Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro Pro Leu Trp 980 985 990 Glu Ser Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn Ile Leu Asn 995 1000 1005 Ser Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val Tyr Asn Glu 1010 1015 1020 Tyr Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln Leu Ser Asp 1025 1030 1035 1040 Leu Arg Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys Asn Ile Gly 1045 1050 1055 Tyr Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile Pro Lys Asn 1060 1065 1070 Lys Leu Phe Asn His Phe Lys Asn His Arg Pro Ala Phe Ser Thr His 1075 1080 1085 Thr Glu Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu Lys Lys Thr 1090 1095 1100 Phe Pro Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn Cys Leu Thr 1105 1110 1115 1120 Gln Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn Ser Ser Val 1125 1130 1135 Pro Ser Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser Tyr Glu Asp 1140 1145 1150 Leu Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu Gln His Leu 1155 1160 1165 Gly Asp Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile Pro His Lys 1170 1175 1180 Asp Val Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro Ala Ser Val 1185 1190 1195 1200 Glu Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn Pro His Val 1205 1210 1215 Lys Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser Thr Tyr Thr 1220 1225 1230 Asp Val Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser Gln His Gly 1235 1240 1245 Arg Glu Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu Gln Val Ser 1250 1255 1260 Asn Ile Leu His Ser Val Phe Ala Asn His Ser Ile Lys Pro Gln Ile 1265 1270 1275 1280 Leu Glu Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser Ile Lys Pro 1285 1290 1295 Val Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr Met Glu Asn 1300 1305 1310 Ile Val Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn Tyr Ser Ser 1315 1320 1325 Pro Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala Glu Tyr Phe 1330 1335 1340 Asp Thr Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr Ile Pro Lys 1345 1350 1355 1360 Tyr Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr Phe Pro Lys 1365 1370 1375 Asp Phe Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu Lys Asp His 1380 1385 1390 Ser Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser Ile Ser Pro 1395 1400 1405 Ser Leu Pro Ser Asn Met Lys Gln Phe Cys Lys Ile Ser Val Trp Leu 1410 1415 1420 Gln Gln His Asp Pro Asp Leu Leu Glu Ile Ile Asn Asn Leu Cys Met 1425 1430 1435 1440 Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys His Gly Val Thr Phe Ile 1445 1450 1455 Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys Lys His Ala Tyr 1460 1465 1470 Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu Ser Leu Ile Leu 1475 1480 1485 Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly Glu Ile Gly Ser 1490 1495 1500 Tyr Thr Gly Val Lys Leu Glu Val Glu Lys Thr Glu Ala Asn Lys Val 1505 1510 1515 1520 Ile Leu Lys Asn Gly Glu Ala Val Leu Val Pro Ala Ala Asp Phe Lys 1525 1530 1535 Pro Phe Pro Asp Arg Arg Leu Ala Val Trp Ile Met Glu Ser Gly Ser 1540 1545 1550 Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys Glu Gly Gly Gly 1555 1560 1565 Asn Asp Pro Pro Val Pro Lys His Ile Ser Pro Tyr Thr Pro Arg Thr 1570 1575 1580 Arg Ile Ala Ile Glu Val Glu Lys Ala Phe Asp Asp Cys Met Arg Gln 1585 1590 1595 1600 Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys Ser Val Ser Leu 1605 1610 1615 Leu Ser Phe Leu Ser Leu Asn His Pro Thr Glu Phe Ile Lys Val Leu 1620 1625 1630 Pro Leu Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr Leu Leu Leu Glu 1635 1640 1645 Pro Tyr Lys Thr His Gly Asp Asp Phe Leu Ile Pro Glu Thr Ile Leu 1650 1655 1660 Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr Gln Ser Ala Met 1665 1670 1675 1680 Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg Gln Thr Phe Met 1685 1690 1695 Asp Ile Ala Asp Ser Ala Thr Lys Glu Val Asp Val Pro Ile Cys Tyr 1700 1705 1710 Ser Asp Pro Glu Thr Val His Ser Tyr Ala Asn His Val Arg Thr Glu 1715 1720 1725 Ile Leu His His Asn Ala Val Asn Lys Val Thr Thr Pro Asn Leu Val 1730 1735 1740 Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr Ile Arg His Tyr 1745 1750 1755 1760 Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu Arg Phe Trp Lys 1765 1770 1775 Lys Leu Trp Gln Asp Glu Gln Arg Phe Val Ile His Gly Leu His Arg 1780 1785 1790 Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu Phe Ala Glu Ile 1795 1800 1805 Val Arg Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn Tyr Ile Asn Glu 1810 1815 1820 Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys His Thr Thr Gly 1825 1830 1835 1840 Asp Ile Ala Pro Asn Asp Arg Phe Ala Met Leu Val Ala Phe Ile Asn 1845 1850 1855 Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu Lys Val Gly Gly 1860 1865 1870 Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp Leu Val Pro Thr Arg Leu 1875 1880 1885 His Ser Phe Leu Asn His Asn Leu Ser Lys Leu Lys Ile Leu Asn Arg 1890 1895 1900 Ala Gln Gln Thr Val Arg Asn Ile Leu Ser Asn Asp Cys Leu Asn Gln 1905 1910 1915 1920 Leu Lys His Tyr Val Lys His Thr Gly Lys Asn Glu Ile Leu Lys Leu 1925 1930 1935 Leu Gln Glu Tyr Phe Leu Val Ala Asp His Arg Glu His His Val Ile 1940 1945 1950 Pro Phe Leu Lys Thr Asp Phe His His Met His Gln Asn Pro Ile Gln 1955 1960 1965 Lys Lys Gln Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr Gly Asp Tyr 1970 1975 1980 Leu Ile Cys Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile Glu Arg Lys 1985 1990 1995 2000 Thr Tyr Lys Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg Tyr Lys Asn 2005 2010 2015 Arg Gln Lys Met Leu Ser Leu Arg Glu Gln Thr Asn Cys Gln Leu Tyr 2020 2025 2030 Phe Phe Val Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys Lys Ile Asn 2035 2040 2045 His Val Ala Tyr Ala Ser Ile Ile Thr Ala Met Thr His Leu Met Val 2050 2055 2060 Arg Asp His Ile Phe Val Ile Gln Thr Lys Asn Glu Ala His Ser Ser 2065 2070 2075 2080 Gln Lys Leu Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu Met Val Cys 2085 2090 2095 Val Val Pro Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu Cys Ile Lys 2100 2105 2110 Leu Trp Ser Ser Leu Ser Gly Ile Ser Gly Val Ile Gly Lys Ile Leu 2115 2120 2125 Ala Asn Thr Cys Ser Val Ala His Leu Val Ser Gly Lys Leu Ser Ser 2130 2135 2140 Gln Asn Ile Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro Phe Pro Lys 2145 2150 2155 2160 Lys Val Lys Arg Met Leu Ile Ser Ile Ser Lys Gly Asn Lys Glu Leu 2165 2170 2175 Glu Ile Lys Leu Leu Ser Gly Val Pro Asn Ile Gly Lys Lys Leu Ala 2180 2185 2190 Ala Glu Ile Leu Lys Asp His Ala Leu Leu Phe Phe Leu Asn Gln Pro 2195 2200 2205 Val Glu Cys Leu Ala Asn Ile Gln Ile Val Gln Lys Thr Arg Thr Ile 2210 2215 2220 Lys Leu Gly Met Lys Arg Ala Glu Ala Ile His Tyr Phe Leu Asn Trp 2225 2230 2235 2240 Cys Gly Ser Ala His Val Thr Asp Asp Ser Gln Asn Ile Thr Glu Ala 2245 2250 2255 Ser Arg Pro Thr Met Gln Val Ala Thr Gln Ser Ala Ala Thr Gln Pro 2260 2265 2270 Ala Ala Thr Gln Pro Leu His Glu Val Ser Asp Asp Ala Ser Ser Asp 2275 2280 2285 Ala Ser Ser Pro Val Gly His Gln Thr Leu Ser Lys Glu Met Leu Leu 2290 2295 2300 Asn Thr Ala Val Glu Thr Gln Met Asp Lys Leu Gly Phe Leu Leu Asn 2305 2310 2315 2320 His Ile Gly Lys Gln Val Thr Thr Lys Val Leu Ser Asn Ala His Ile 2325 2330 2335 Thr Gln Thr Met Lys Glu Ile Ile Leu Glu Asn His Ser Val Asp Gly 2340 2345 2350 Gly Ala Ala Lys Asn Val Ser Lys Gly Lys Ser Ser Pro Lys Glu Lys 2355 2360 2365 Lys His Trp Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys Ser Lys 2370 2375 2380 Arg Ser Phe Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val Asn Thr 2385 2390 2395 2400 Leu Leu Leu Asn Trp Asp Asn Val Arg Gly Ala Ile Lys Lys Phe Leu 2405 2410 2415 Asp Asp Asp Arg Glu Trp Cys Gly Arg Ile Asn Met Ile Asn Gly Val 2420 2425 2430 Pro Glu Ile Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly Glu Asn 2435 2440 2445 Ile Tyr Phe Gly Ser Glu Ala Met Met Pro Ala Asp Ile Tyr Ser Arg 2450 2455 2460 Val Ala Asn Lys Pro Ala Met Phe Val Phe His Thr His Pro Asn Leu 2465 2470 2475 2480 Gly Ser Cys Cys Gly Gly Met Pro Ser Ile Cys Asp Ile Ser Thr Thr 2485 2490 2495 Leu Arg Tyr Leu Leu Met Gly Trp Thr Ala Gly His Leu Ile Ile Ser 2500 2505 2510 Ser Asn Gln Val Gly Met Leu Thr Val Asp Lys Arg Ile Ile Val Asp 2515 2520 2525 Leu Trp Ala Asn Glu Asn Pro Arg Trp Leu Met Ala Gln Lys Ile Leu 2530 2535 2540 Asp Ile Phe Met Met Leu Thr Ser Arg Arg Ser Leu Val Asn Pro Trp 2545 2550 2555 2560 Thr Leu Arg Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile Glu Tyr 2565 2570 2575 Ile Ile Phe Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu Arg Leu 2580 2585 2590 Leu Met Phe Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu His Glu Leu 2595 2600 2605 Leu Asp Asp Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr Thr Ser 2610 2615 2620 Thr Lys Lys Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr Ser Leu Val 2625 2630 2635 2640 Ala Ala Asp Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg Ile Phe Thr 2645 2650 2655 Ser Gln Gln Lys Ile Ile Gln Asn Ala Leu Lys His Asn Gln Glu Val 2660 2665 2670 Ile Ile Pro Pro Gly Ile Lys Phe Thr Val Val Thr Val Lys Ala Lys 2675 2680 2685 Pro Ala Arg Gln Gly His Asn Pro Ala Thr Gly Glu Pro Ile Gln Ile 2690 2695 2700 Lys Ala Lys Pro Glu His Lys Ala Val Lys Ile Arg Ala Leu Lys Pro 2705 2710 2715 2720 Val His Asp Met Leu Asn Val Glu Pro Arg Glu Gln Phe Phe Gln Asp 2725 2730 2735 Leu Leu Ser Ala Val Asp Gln Gln Met Asp Thr Val Lys Asn Asp Ile 2740 2745 2750 Lys Asp Ile Met Lys Glu Lys Thr Ser Phe Met Val Ser Phe Glu Asn 2755 2760 2765 Phe Ile Glu Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln Asp Leu Gln 2770 2775 2780 Asn Lys Tyr Glu Glu Met Ala Ala Asn Leu Met Thr Val Met Thr Asp 2785 2790 2795 2800 Thr Lys Ile Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu Ile Leu Met 2805 2810 2815 Ile Asn Gly Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile Lys Glu Ala 2820 2825 2830 Met Pro Leu Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr Ser Pro Pro 2835 2840 2845 Ala Ser Gly Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro Thr Asn Ala 2850 2855 2860 Met Phe Phe Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr Phe Arg Glu 2865 2870 2875 2880 Lys Phe Leu Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu Gln Phe Ala 2885 2890 2895 Asn Lys Thr Gly Ile Glu Arg Leu His Ala Glu Gly Leu Tyr Met Trp 2900 2905 2910 Arg Thr Gln Phe Ser Asp Glu Gln Lys Lys Met Val Lys Glu Met Met 2915 2920 2925 Lys Lys 2930 <210> 27 <211> 973 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 2' <400> 27 Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 1 5 10 15 Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu Lys 20 25 30 Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 35 40 45 Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 50 55 60 Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Asp Phe Ile Leu Asn 65 70 75 80 Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser Ser 85 90 95 Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser Val 100 105 110 Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr Leu 115 120 125 Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr Ala 130 135 140 Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met Ile 145 150 155 160 Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser Glu 165 170 175 Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser Ser 180 185 190 Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Thr Pro Lys Asp 195 200 205 Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu Gln 210 215 220 Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn Phe 225 230 235 240 Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu Val 245 250 255 Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys Lys Asp Ser Glu Phe 260 265 270 Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu Cys Leu Ser Pro Val 275 280 285 Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr Thr Ile Leu Ile Ala 290 295 300 Ile Val Val Leu Val Ile Ile Ile Ile Val Leu Ile Tyr Leu Phe Ser 305 310 315 320 Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu Glu Glu Asp Ile Gln 325 330 335 Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala Glu Val Thr Pro Gln 340 345 350 Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr Ala Ser Val Gly Lys 355 360 365 Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro Ala Thr Asn Lys Pro 370 375 380 Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val Met Ala Thr Gly Gly 385 390 395 400 Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro Ala His Pro Ala Glu 405 410 415 Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala Ser Gln Thr Met Ser 420 425 430 Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr Thr His Lys Asp Leu 435 440 445 Glu Asn Ser Leu Phe Ser Lys Lys Lys Tyr Ile Gly Leu Ile Asp Lys 450 455 460 Lys Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr Ser Ile Leu Ile Ile 465 470 475 480 Gly Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu Ile Tyr Asn Phe Ile 485 490 495 Gly Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn Asp Asp Lys Met Phe 500 505 510 Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr Phe 515 520 525 Ser Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser Asn Tyr Cys Lys Gln 530 535 540 Leu Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn Leu Val Asn Leu Thr 545 550 555 560 Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr Trp 565 570 575 Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asn Ser 580 585 590 Gly Tyr Tyr Lys Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys Ser 595 600 605 Lys Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn Ile Val Leu Ser Ile 610 615 620 Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile Leu Asp Ser Asn Ile 625 630 635 640 Thr Asn Asp Asn Asn Asp Ile Asn Gly Val Ser Trp Asn Thr Tyr Phe 645 650 655 Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly Lys Ala Gly Asn Phe 660 665 670 Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu Tyr Asn Ile Thr Asn Asn 675 680 685 Cys Ser Leu Thr Ile Phe Pro His Asn Thr Asp Ile Phe Asn Thr Thr 690 695 700 Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile Asn Tyr Thr Ile Lys Leu 705 710 715 720 Leu Thr Pro Val Asp Ser Pro Asn Ile Thr Tyr Asn Cys Thr Asn Ser 725 730 735 Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn Thr Asn Ile Tyr Leu 740 745 750 Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr Asn Glu Ser Ile Leu Asn 755 760 765 Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn Phe Thr Ala Thr Cys Ile 770 775 780 Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu Thr Glu Leu Ile Asn Cys 785 790 795 800 Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr Phe Tyr Thr Phe Phe Lys 805 810 815 Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser Gly Ile Ile Ile Ser Ile 820 825 830 Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser Leu Arg Lys Arg Lys Lys 835 840 845 His Val Glu Glu Ile Glu Ser Pro Pro Pro Ser Glu Ser Asn Glu Glu 850 855 860 Glu Gln Cys Gln His Asp Asp Thr Thr Ser Ile His Glu Pro Ser Pro 865 870 875 880 Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn Thr 885 890 895 Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe Pro 900 905 910 Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro 915 920 925 Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro Pro 930 935 940 Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu Ser 945 950 955 960 Thr Gln Asn Ile Ser Leu Ile His Val Asp Arg Ile Ile 965 970 <210> 28 <211> 2032 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 3' <400> 28 Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 1 5 10 15 Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu Lys 20 25 30 Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 35 40 45 Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 50 55 60 Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Tyr His Asp Tyr Ala 65 70 75 80 Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro Pro Leu Trp Glu Ser 85 90 95 Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn Ile Leu Asn Ser Arg 100 105 110 Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val Tyr Asn Glu Tyr Thr 115 120 125 Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln Leu Ser Asp Leu Arg 130 135 140 Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys Asn Ile Gly Tyr Gly 145 150 155 160 Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile Pro Lys Asn Lys Leu 165 170 175 Phe Asn His Phe Lys Asn His Arg Pro Ala Phe Ser Thr His Thr Glu 180 185 190 Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu Lys Lys Thr Phe Pro 195 200 205 Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn Cys Leu Thr Gln Pro 210 215 220 Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn Ser Ser Val Pro Ser 225 230 235 240 Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser Tyr Glu Asp Leu Pro 245 250 255 Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu Gln His Leu Gly Asp 260 265 270 Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile Pro His Lys Asp Val 275 280 285 Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro Ala Ser Val Glu Lys 290 295 300 Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn Pro His Val Lys Pro 305 310 315 320 Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser Thr Tyr Thr Asp Val 325 330 335 Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser Gln His Gly Arg Glu 340 345 350 Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu Gln Val Ser Asn Ile 355 360 365 Leu His Ser Val Phe Ala Asn His Ser Ile Lys Pro Gln Ile Leu Glu 370 375 380 Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser Ile Lys Pro Val Ser 385 390 395 400 Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr Met Glu Asn Ile Val 405 410 415 Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn Tyr Ser Ser Pro Ile 420 425 430 Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala Glu Tyr Phe Asp Thr 435 440 445 Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr Ile Pro Lys Tyr Thr 450 455 460 Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr Phe Pro Lys Asp Phe 465 470 475 480 Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu Lys Asp His Ser Leu 485 490 495 Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser Ile Ser Pro Ser Leu 500 505 510 Pro Ser Asn Met Lys Gln Phe Cys Lys Ile Ser Val Trp Leu Gln Gln 515 520 525 His Asp Pro Asp Leu Leu Glu Ile Ile Asn Asn Leu Cys Met Leu Gly 530 535 540 Asn Leu Ser Ala Ala Lys Tyr Lys His Gly Val Thr Phe Ile Tyr Pro 545 550 555 560 Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys Lys His Ala Tyr Ser Asn 565 570 575 Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu Ser Leu Ile Leu Pro Phe 580 585 590 Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly Glu Ile Gly Ser Tyr Thr 595 600 605 Gly Val Lys Leu Glu Val Glu Lys Thr Glu Ala Asn Lys Val Ile Leu 610 615 620 Lys Asn Gly Glu Ala Val Leu Val Pro Ala Ala Asp Phe Lys Pro Phe 625 630 635 640 Pro Asp Arg Arg Leu Ala Val Trp Ile Met Glu Ser Gly Ser Met Pro 645 650 655 Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys Glu Gly Gly Gly Asn Asp 660 665 670 Pro Pro Val Pro Lys His Ile Ser Pro Tyr Thr Pro Arg Thr Arg Ile 675 680 685 Ala Ile Glu Val Glu Lys Ala Phe Asp Asp Cys Met Arg Gln Asn Trp 690 695 700 Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys Ser Val Ser Leu Leu Ser 705 710 715 720 Phe Leu Ser Leu Asn His Pro Thr Glu Phe Ile Lys Val Leu Pro Leu 725 730 735 Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr Leu Leu Leu Glu Pro Tyr 740 745 750 Lys Thr His Gly Asp Asp Phe Leu Ile Pro Glu Thr Ile Leu Phe Gly 755 760 765 Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr Gln Ser Ala Met Leu Glu 770 775 780 Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg Gln Thr Phe Met Asp Ile 785 790 795 800 Ala Asp Ser Ala Thr Lys Glu Val Asp Val Pro Ile Cys Tyr Ser Asp 805 810 815 Pro Glu Thr Val His Ser Tyr Ala Asn His Val Arg Thr Glu Ile Leu 820 825 830 His His Asn Ala Val Asn Lys Val Thr Thr Pro Asn Leu Val Val Gln 835 840 845 Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr Ile Arg His Tyr Gly Pro 850 855 860 Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu Arg Phe Trp Lys Lys Leu 865 870 875 880 Trp Gln Asp Glu Gln Arg Phe Val Ile His Gly Leu His Arg Thr Leu 885 890 895 Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu Phe Ala Glu Ile Val Arg 900 905 910 Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn Tyr Ile Asn Glu Leu Asn 915 920 925 Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys His Thr Thr Gly Asp Ile 930 935 940 Ala Pro Asn Asp Arg Phe Ala Met Leu Val Ala Phe Ile Asn Ser Thr 945 950 955 960 Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu Lys Val Gly Gly Asn Glu 965 970 975 Thr Gln Thr Ser Ser Leu Thr Asp Leu Val Pro Thr Arg Leu His Ser 980 985 990 Phe Leu Asn His Asn Leu Ser Lys Leu Lys Ile Leu Asn Arg Ala Gln 995 1000 1005 Gln Thr Val Arg Asn Ile Leu Ser Asn Asp Cys Leu Asn Gln Leu Lys 1010 1015 1020 His Tyr Val Lys His Thr Gly Lys Asn Glu Ile Leu Lys Leu Leu Gln 1025 1030 1035 1040 Glu Tyr Phe Leu Val Ala Asp His Arg Glu His His Val Ile Pro Phe 1045 1050 1055 Leu Lys Thr Asp Phe His His Met His Gln Asn Pro Ile Gln Lys Lys 1060 1065 1070 Gln Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr Gly Asp Tyr Leu Ile 1075 1080 1085 Cys Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile Glu Arg Lys Thr Tyr 1090 1095 1100 Lys Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg Tyr Lys Asn Arg Gln 1105 1110 1115 1120 Lys Met Leu Ser Leu Arg Glu Gln Thr Asn Cys Gln Leu Tyr Phe Phe 1125 1130 1135 Val Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys Lys Ile Asn His Val 1140 1145 1150 Ala Tyr Ala Ser Ile Ile Thr Ala Met Thr His Leu Met Val Arg Asp 1155 1160 1165 His Ile Phe Val Ile Gln Thr Lys Asn Glu Ala His Ser Ser Gln Lys 1170 1175 1180 Leu Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu Met Val Cys Val Val 1185 1190 1195 1200 Pro Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu Cys Ile Lys Leu Trp 1205 1210 1215 Ser Ser Leu Ser Gly Ile Ser Gly Val Ile Gly Lys Ile Leu Ala Asn 1220 1225 1230 Thr Cys Ser Val Ala His Leu Val Ser Gly Lys Leu Ser Ser Gln Asn 1235 1240 1245 Ile Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro Phe Pro Lys Lys Val 1250 1255 1260 Lys Arg Met Leu Ile Ser Ile Ser Lys Gly Asn Lys Glu Leu Glu Ile 1265 1270 1275 1280 Lys Leu Leu Ser Gly Val Pro Asn Ile Gly Lys Lys Leu Ala Ala Glu 1285 1290 1295 Ile Leu Lys Asp His Ala Leu Leu Phe Phe Leu Asn Gln Pro Val Glu 1300 1305 1310 Cys Leu Ala Asn Ile Gln Ile Val Gln Lys Thr Arg Thr Ile Lys Leu 1315 1320 1325 Gly Met Lys Arg Ala Glu Ala Ile His Tyr Phe Leu Asn Trp Cys Gly 1330 1335 1340 Ser Ala His Val Thr Asp Asp Ser Gln Asn Ile Thr Glu Ala Ser Arg 1345 1350 1355 1360 Pro Thr Met Gln Val Ala Thr Gln Ser Ala Ala Thr Gln Pro Ala Ala 1365 1370 1375 Thr Gln Pro Leu His Glu Val Ser Asp Asp Ala Ser Ser Asp Ala Ser 1380 1385 1390 Ser Pro Val Gly His Gln Thr Leu Ser Lys Glu Met Leu Leu Asn Thr 1395 1400 1405 Ala Val Glu Thr Gln Met Asp Lys Leu Gly Phe Leu Leu Asn His Ile 1410 1415 1420 Gly Lys Gln Val Thr Thr Lys Val Leu Ser Asn Ala His Ile Thr Gln 1425 1430 1435 1440 Thr Met Lys Glu Ile Ile Leu Glu Asn His Ser Val Asp Gly Gly Ala 1445 1450 1455 Ala Lys Asn Val Ser Lys Gly Lys Ser Ser Pro Lys Glu Lys Lys His 1460 1465 1470 Trp Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys Ser Lys Arg Ser 1475 1480 1485 Phe Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val Asn Thr Leu Leu 1490 1495 1500 Leu Asn Trp Asp Asn Val Arg Gly Ala Ile Lys Lys Phe Leu Asp Asp 1505 1510 1515 1520 Asp Arg Glu Trp Cys Gly Arg Ile Asn Met Ile Asn Gly Val Pro Glu 1525 1530 1535 Ile Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly Glu Asn Ile Tyr 1540 1545 1550 Phe Gly Ser Glu Ala Met Met Pro Ala Asp Ile Tyr Ser Arg Val Ala 1555 1560 1565 Asn Lys Pro Ala Met Phe Val Phe His Thr His Pro Asn Leu Gly Ser 1570 1575 1580 Cys Cys Gly Gly Met Pro Ser Ile Cys Asp Ile Ser Thr Thr Leu Arg 1585 1590 1595 1600 Tyr Leu Leu Met Gly Trp Thr Ala Gly His Leu Ile Ile Ser Ser Asn 1605 1610 1615 Gln Val Gly Met Leu Thr Val Asp Lys Arg Ile Ile Val Asp Leu Trp 1620 1625 1630 Ala Asn Glu Asn Pro Arg Trp Leu Met Ala Gln Lys Ile Leu Asp Ile 1635 1640 1645 Phe Met Met Leu Thr Ser Arg Arg Ser Leu Val Asn Pro Trp Thr Leu 1650 1655 1660 Arg Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile Glu Tyr Ile Ile 1665 1670 1675 1680 Phe Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu Arg Leu Leu Met 1685 1690 1695 Phe Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu His Glu Leu Leu Asp 1700 1705 1710 Asp Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr Thr Ser Thr Lys 1715 1720 1725 Lys Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr Ser Leu Val Ala Ala 1730 1735 1740 Asp Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg Ile Phe Thr Ser Gln 1745 1750 1755 1760 Gln Lys Ile Ile Gln Asn Ala Leu Lys His Asn Gln Glu Val Ile Ile 1765 1770 1775 Pro Pro Gly Ile Lys Phe Thr Val Val Thr Val Lys Ala Lys Pro Ala 1780 1785 1790 Arg Gln Gly His Asn Pro Ala Thr Gly Glu Pro Ile Gln Ile Lys Ala 1795 1800 1805 Lys Pro Glu His Lys Ala Val Lys Ile Arg Ala Leu Lys Pro Val His 1810 1815 1820 Asp Met Leu Asn Val Glu Pro Arg Glu Gln Phe Phe Gln Asp Leu Leu 1825 1830 1835 1840 Ser Ala Val Asp Gln Gln Met Asp Thr Val Lys Asn Asp Ile Lys Asp 1845 1850 1855 Ile Met Lys Glu Lys Thr Ser Phe Met Val Ser Phe Glu Asn Phe Ile 1860 1865 1870 Glu Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln Asp Leu Gln Asn Lys 1875 1880 1885 Tyr Glu Glu Met Ala Ala Asn Leu Met Thr Val Met Thr Asp Thr Lys 1890 1895 1900 Ile Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu Ile Leu Met Ile Asn 1905 1910 1915 1920 Gly Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile Lys Glu Ala Met Pro 1925 1930 1935 Leu Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr Ser Pro Pro Ala Ser 1940 1945 1950 Gly Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro Thr Asn Ala Met Phe 1955 1960 1965 Phe Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr Phe Arg Glu Lys Phe 1970 1975 1980 Leu Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu Gln Phe Ala Asn Lys 1985 1990 1995 2000 Thr Gly Ile Glu Arg Leu His Ala Glu Gly Leu Tyr Met Trp Arg Thr 2005 2010 2015 Gln Phe Ser Asp Glu Gln Lys Lys Met Val Lys Glu Met Met Lys Lys 2020 2025 2030 <210> 29 <211> 1502 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 4' <400> 29 Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 1 5 10 15 Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu Lys 20 25 30 Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 35 40 45 Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 50 55 60 Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Asp Phe Ile Leu Asn 65 70 75 80 Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser Ser 85 90 95 Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser Val 100 105 110 Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr Leu 115 120 125 Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr Ala 130 135 140 Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met Ile 145 150 155 160 Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser Glu 165 170 175 Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser Ser 180 185 190 Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Thr Pro Lys Asp 195 200 205 Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu Gln 210 215 220 Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn Phe 225 230 235 240 Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu Val 245 250 255 Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys Lys Asp Ser Glu Phe 260 265 270 Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu Cys Leu Ser Pro Val 275 280 285 Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr Thr Ile Leu Ile Ala 290 295 300 Ile Val Val Leu Val Ile Ile Ile Ile Val Leu Ile Tyr Leu Phe Ser 305 310 315 320 Ser Arg Lys Lys Lys Ala Ala Ala Ala Ile Glu Glu Glu Asp Ile Gln 325 330 335 Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Ala Glu Val Thr Pro Gln 340 345 350 Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr Ala Ser Val Gly Lys 355 360 365 Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro Ala Thr Asn Lys Pro 370 375 380 Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val Met Ala Thr Gly Gly 385 390 395 400 Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro Ala His Pro Ala Glu 405 410 415 Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala Ser Gln Thr Met Ser 420 425 430 Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr Thr His Lys Asp Leu 435 440 445 Glu Asn Ser Leu Pro Ser Asn Met Lys Gln Phe Cys Lys Ile Ser Val 450 455 460 Trp Leu Gln Gln His Asp Pro Asp Leu Leu Glu Ile Ile Asn Asn Leu 465 470 475 480 Cys Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys His Gly Val Thr 485 490 495 Phe Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys Lys His 500 505 510 Ala Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu Ser Leu 515 520 525 Ile Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly Glu Ile 530 535 540 Gly Ser Tyr Thr Gly Val Lys Leu Glu Val Glu Lys Thr Glu Ala Asn 545 550 555 560 Lys Val Ile Leu Lys Asn Gly Glu Ala Val Leu Val Pro Ala Ala Asp 565 570 575 Phe Lys Pro Phe Pro Asp Arg Arg Leu Ala Val Trp Ile Met Glu Ser 580 585 590 Gly Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys Glu Gly 595 600 605 Gly Gly Asn Asp Pro Pro Val Pro Lys His Ile Ser Pro Tyr Thr Pro 610 615 620 Arg Thr Arg Ile Ala Ile Glu Val Glu Lys Ala Phe Asp Asp Cys Met 625 630 635 640 Arg Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys Ser Val 645 650 655 Ser Leu Leu Ser Phe Leu Ser Leu Asn His Pro Thr Glu Phe Ile Lys 660 665 670 Val Leu Pro Leu Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr Leu Leu 675 680 685 Leu Glu Pro Tyr Lys Thr His Gly Asp Asp Phe Leu Ile Pro Glu Thr 690 695 700 Ile Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr Gln Ser 705 710 715 720 Ala Met Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg Gln Thr 725 730 735 Phe Met Asp Ile Ala Asp Ser Ala Thr Lys Glu Val Asp Val Pro Ile 740 745 750 Cys Tyr Ser Asp Pro Glu Thr Val His Ser Tyr Ala Asn His Val Arg 755 760 765 Thr Glu Ile Leu His His Asn Ala Val Asn Lys Val Thr Thr Pro Asn 770 775 780 Leu Val Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr Ile Arg 785 790 795 800 His Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu Arg Phe 805 810 815 Trp Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe Val Ile His Gly Leu 820 825 830 His Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu Phe Ala 835 840 845 Glu Ile Val Arg Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn Tyr Ile 850 855 860 Asn Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys His Thr 865 870 875 880 Thr Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala Met Leu Val Ala Phe 885 890 895 Ile Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu Lys Val 900 905 910 Gly Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp Leu Val Pro Thr 915 920 925 Arg Leu His Ser Phe Leu Asn His Asn Leu Ser Lys Leu Lys Ile Leu 930 935 940 Asn Arg Ala Gln Gln Thr Val Arg Asn Ile Leu Ser Asn Asp Cys Leu 945 950 955 960 Asn Gln Leu Lys His Tyr Val Lys His Thr Gly Lys Asn Glu Ile Leu 965 970 975 Lys Leu Leu Gln Glu Phe Ser Lys Lys Lys Tyr Ile Gly Leu Ile Asp 980 985 990 Lys Lys Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr Ser Ile Leu Ile 995 1000 1005 Ile Gly Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu Ile Tyr Asn Phe 1010 1015 1020 Ile Gly Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn Asp Asp Lys Met 1025 1030 1035 1040 Phe Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr 1045 1050 1055 Phe Ser Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser Asn Tyr Cys Lys 1060 1065 1070 Gln Leu Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn Leu Val Asn Leu 1075 1080 1085 Thr Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr 1090 1095 1100 Trp Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asn 1105 1110 1115 1120 Ser Gly Tyr Tyr Lys Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys 1125 1130 1135 Ser Lys Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn Ile Val Leu Ser 1140 1145 1150 Ile Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile Leu Asp Ser Asn 1155 1160 1165 Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly Val Ser Trp Asn Thr Tyr 1170 1175 1180 Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly Lys Ala Gly Asn 1185 1190 1195 1200 Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu Tyr Asn Ile Thr Asn 1205 1210 1215 Asn Cys Ser Leu Thr Ile Phe Pro His Asn Thr Asp Ile Phe Asn Thr 1220 1225 1230 Thr Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile Asn Tyr Thr Ile Lys 1235 1240 1245 Leu Leu Thr Pro Val Asp Ser Pro Asn Ile Thr Tyr Asn Cys Thr Asn 1250 1255 1260 Ser Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn Thr Asn Ile Tyr 1265 1270 1275 1280 Leu Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr Asn Glu Ser Ile Leu 1285 1290 1295 Asn Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn Phe Thr Ala Thr Cys 1300 1305 1310 Ile Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu Thr Glu Leu Ile Asn 1315 1320 1325 Cys Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr Phe Tyr Thr Phe Phe 1330 1335 1340 Lys Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser Gly Ile Ile Ile Ser 1345 1350 1355 1360 Ile Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser Leu Arg Lys Arg Lys 1365 1370 1375 Lys His Val Glu Glu Ile Glu Ser Pro Pro Pro Ser Glu Ser Asn Glu 1380 1385 1390 Glu Glu Gln Cys Gln His Asp Asp Thr Thr Ser Ile His Glu Pro Ser 1395 1400 1405 Pro Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn 1410 1415 1420 Thr Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe 1425 1430 1435 1440 Pro Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys 1445 1450 1455 Pro Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro 1460 1465 1470 Pro Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu 1475 1480 1485 Ser Thr Gln Asn Ile Ser Leu Ile His Val Asp Arg Ile Ile 1490 1495 1500 <210> 30 <211> 1503 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 5' <400> 30 Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 1 5 10 15 Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu Lys 20 25 30 Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 35 40 45 Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 50 55 60 Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Tyr His Asp Tyr Ala 65 70 75 80 Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro Pro Leu Trp Glu Ser 85 90 95 Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn Ile Leu Asn Ser Arg 100 105 110 Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val Tyr Asn Glu Tyr Thr 115 120 125 Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln Leu Ser Asp Leu Arg 130 135 140 Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys Asn Ile Gly Tyr Gly 145 150 155 160 Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile Pro Lys Asn Lys Leu 165 170 175 Phe Asn His Phe Lys Asn His Arg Pro Ala Phe Ser Thr His Thr Glu 180 185 190 Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu Lys Lys Thr Phe Pro 195 200 205 Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn Cys Leu Thr Gln Pro 210 215 220 Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn Ser Ser Val Pro Ser 225 230 235 240 Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser Tyr Glu Asp Leu Pro 245 250 255 Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu Gln His Leu Gly Asp 260 265 270 Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile Pro His Lys Asp Val 275 280 285 Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro Ala Ser Val Glu Lys 290 295 300 Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn Pro His Val Lys Pro 305 310 315 320 Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser Thr Tyr Thr Asp Val 325 330 335 Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser Gln His Gly Arg Glu 340 345 350 Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu Gln Val Ser Asn Ile 355 360 365 Leu His Ser Val Phe Ala Asn His Ser Ile Lys Pro Gln Ile Leu Glu 370 375 380 Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser Ile Lys Pro Val Ser 385 390 395 400 Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr Met Glu Asn Ile Val 405 410 415 Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn Tyr Ser Ser Pro Ile 420 425 430 Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala Glu Tyr Phe Asp Thr 435 440 445 Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr Ile Pro Lys Tyr Thr 450 455 460 Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr Phe Pro Lys Asp Phe 465 470 475 480 Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu Lys Asp His Ser Leu 485 490 495 Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser Ile Ser Pro Ser Leu 500 505 510 Tyr Phe Leu Val Ala Asp His Arg Glu His His Val Ile Pro Phe Leu 515 520 525 Lys Thr Asp Phe His His Met His Gln Asn Pro Ile Gln Lys Lys Gln 530 535 540 Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr Gly Asp Tyr Leu Ile Cys 545 550 555 560 Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile Glu Arg Lys Thr Tyr Lys 565 570 575 Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg Tyr Lys Asn Arg Gln Lys 580 585 590 Met Leu Ser Leu Arg Glu Gln Thr Asn Cys Gln Leu Tyr Phe Phe Val 595 600 605 Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys Lys Ile Asn His Val Ala 610 615 620 Tyr Ala Ser Ile Ile Thr Ala Met Thr His Leu Met Val Arg Asp His 625 630 635 640 Ile Phe Val Ile Gln Thr Lys Asn Glu Ala His Ser Ser Gln Lys Leu 645 650 655 Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu Met Val Cys Val Val Pro 660 665 670 Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu Cys Ile Lys Leu Trp Ser 675 680 685 Ser Leu Ser Gly Ile Ser Gly Val Ile Gly Lys Ile Leu Ala Asn Thr 690 695 700 Cys Ser Val Ala His Leu Val Ser Gly Lys Leu Ser Ser Gln Asn Ile 705 710 715 720 Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro Phe Pro Lys Lys Val Lys 725 730 735 Arg Met Leu Ile Ser Ile Ser Lys Gly Asn Lys Glu Leu Glu Ile Lys 740 745 750 Leu Leu Ser Gly Val Pro Asn Ile Gly Lys Lys Leu Ala Ala Glu Ile 755 760 765 Leu Lys Asp His Ala Leu Leu Phe Phe Leu Asn Gln Pro Val Glu Cys 770 775 780 Leu Ala Asn Ile Gln Ile Val Gln Lys Thr Arg Thr Ile Lys Leu Gly 785 790 795 800 Met Lys Arg Ala Glu Ala Ile His Tyr Phe Leu Asn Trp Cys Gly Ser 805 810 815 Ala His Val Thr Asp Asp Ser Gln Asn Ile Thr Glu Ala Ser Arg Pro 820 825 830 Thr Met Gln Val Ala Thr Gln Ser Ala Ala Thr Gln Pro Ala Ala Thr 835 840 845 Gln Pro Leu His Glu Val Ser Asp Asp Ala Ser Ser Asp Ala Ser Ser 850 855 860 Pro Val Gly His Gln Thr Leu Ser Lys Glu Met Leu Leu Asn Thr Ala 865 870 875 880 Val Glu Thr Gln Met Asp Lys Leu Gly Phe Leu Leu Asn His Ile Gly 885 890 895 Lys Gln Val Thr Thr Lys Val Leu Ser Asn Ala His Ile Thr Gln Thr 900 905 910 Met Lys Glu Ile Ile Leu Glu Asn His Ser Val Asp Gly Gly Ala Ala 915 920 925 Lys Asn Val Ser Lys Gly Lys Ser Ser Pro Lys Glu Lys Lys His Trp 930 935 940 Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys Ser Lys Arg Ser Phe 945 950 955 960 Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val Asn Thr Leu Leu Leu 965 970 975 Asn Trp Asp Asn Val Arg Gly Ala Ile Lys Lys Phe Leu Asp Asp Asp 980 985 990 Arg Glu Trp Cys Gly Arg Ile Asn Met Ile Asn Gly Val Pro Glu Ile 995 1000 1005 Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly Glu Asn Ile Tyr Phe 1010 1015 1020 Gly Ser Glu Ala Met Met Pro Ala Asp Ile Tyr Ser Arg Val Ala Asn 1025 1030 1035 1040 Lys Pro Ala Met Phe Val Phe His Thr His Pro Asn Leu Gly Ser Cys 1045 1050 1055 Cys Gly Gly Met Pro Ser Ile Cys Asp Ile Ser Thr Thr Leu Arg Tyr 1060 1065 1070 Leu Leu Met Gly Trp Thr Ala Gly His Leu Ile Ile Ser Ser Asn Gln 1075 1080 1085 Val Gly Met Leu Thr Val Asp Lys Arg Ile Ile Val Asp Leu Trp Ala 1090 1095 1100 Asn Glu Asn Pro Arg Trp Leu Met Ala Gln Lys Ile Leu Asp Ile Phe 1105 1110 1115 1120 Met Met Leu Thr Ser Arg Arg Ser Leu Val Asn Pro Trp Thr Leu Arg 1125 1130 1135 Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile Glu Tyr Ile Ile Phe 1140 1145 1150 Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu Arg Leu Leu Met Phe 1155 1160 1165 Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu His Glu Leu Leu Asp Asp 1170 1175 1180 Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr Thr Ser Thr Lys Lys 1185 1190 1195 1200 Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr Ser Leu Val Ala Ala Asp 1205 1210 1215 Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg Ile Phe Thr Ser Gln Gln 1220 1225 1230 Lys Ile Ile Gln Asn Ala Leu Lys His Asn Gln Glu Val Ile Ile Pro 1235 1240 1245 Pro Gly Ile Lys Phe Thr Val Val Thr Val Lys Ala Lys Pro Ala Arg 1250 1255 1260 Gln Gly His Asn Pro Ala Thr Gly Glu Pro Ile Gln Ile Lys Ala Lys 1265 1270 1275 1280 Pro Glu His Lys Ala Val Lys Ile Arg Ala Leu Lys Pro Val His Asp 1285 1290 1295 Met Leu Asn Val Glu Pro Arg Glu Gln Phe Phe Gln Asp Leu Leu Ser 1300 1305 1310 Ala Val Asp Gln Gln Met Asp Thr Val Lys Asn Asp Ile Lys Asp Ile 1315 1320 1325 Met Lys Glu Lys Thr Ser Phe Met Val Ser Phe Glu Asn Phe Ile Glu 1330 1335 1340 Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln Asp Leu Gln Asn Lys Tyr 1345 1350 1355 1360 Glu Glu Met Ala Ala Asn Leu Met Thr Val Met Thr Asp Thr Lys Ile 1365 1370 1375 Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu Ile Leu Met Ile Asn Gly 1380 1385 1390 Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile Lys Glu Ala Met Pro Leu 1395 1400 1405 Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr Ser Pro Pro Ala Ser Gly 1410 1415 1420 Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro Thr Asn Ala Met Phe Phe 1425 1430 1435 1440 Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr Phe Arg Glu Lys Phe Leu 1445 1450 1455 Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu Gln Phe Ala Asn Lys Thr 1460 1465 1470 Gly Ile Glu Arg Leu His Ala Glu Gly Leu Tyr Met Trp Arg Thr Gln 1475 1480 1485 Phe Ser Asp Glu Gln Lys Lys Met Val Lys Glu Met Met Lys Lys 1490 1495 1500 <210> 31 <211> 8565 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 1 <400> 31 gattttattt taaatatatc catgaaaatg gaggtcatct tcaaaacgga tttaagatca 60 tcttcacaag ttgtgtttca tgcgggtagc ctgtataatt ggttttctgt tgagattatc 120 aatagcggta gaattgttac gaccgctata aaaacattgc ttagtactgt taagtatgat 180 attgtgaaat ctgctcgtat atatgcaggg caagggtata ctgaacatca ggctcaagaa 240 gaatggaata tgattctgca tgtgctgttt gaagaggaga cggaatcctc agcatcttcg 300 gagtcaattc atgaaaaaaa tgataatgaa accaatgaat gcacatcctc ctttgaaacg 360 ttgtttgagc aagagccctc atcggagaca cctaaagact ccaagctgta tatgcttgca 420 caaaagactg tgcaacatat tgaacaatat ggaaaggcac ctgattttaa caaggttatt 480 agagcacata attttattca aaccattcat ggaacccctc taaaggaaga agaaaaagag 540 gtggtaagac tcatggttat taaactttta aaaaaaaaag attctgaatt ttttcaaccg 600 gtttatccgc ggcattatgg tgagtgtttg tcaccagtca ctacaccaag cttcttctcc 660 acacatatgt atactattct cattgctatc gtggtcttag tcatcattat catcgttcta 720 atctatctat tctcttcaag aaagaaaaaa gctgctgctg ctattgagga ggaagatata 780 cagtttataa atccttatca agatcagcag tgggcagaag tcactccaca accaggtacc 840 tctaaaccag ctggagcgac tacagcaagt gtaggcaagc cagtcacggg cagaccggca 900 acaaacagac cagcaacaaa caaaccagtt acggacaacc cagttacgga cagactagtc 960 atggcaactg gcgggccggc ggccgcacct gcggccgcga gtgctcctgc tcatccggct 1020 gagccttaca cgacagtcac tactcagaac actgcttcac aaacaatgtc ggctattgaa 1080 aatttacgac aaagaaacac ctatacgcat aaagacctag aaaactcctt gttttctaag 1140 aaaaagtaca tcggtcttat cgataagaag tgtgaattga aaaaaatact agatgattat 1200 agtatattaa taattggaat attaattgga actaacatca taacccttat ttataatttt 1260 ataggatgga ttaattatat aaaatgttac caaaatgatg ataagatgtt ttattgccct 1320 aaagattggg ttggatataa taatgtttgt tattatttta gtaatgaaga aaaaaattat 1380 actaatgcaa gtaattattg taagcaatta aatagtacgc ttgctaataa taatactaat 1440 ttagtaaatc ttactaaaac attaaatctt actaaaacat ataatcacga atctaattat 1500 tgggttaatt attctttaat taaaaatgag tcagtactat tacgtaatag tggatattac 1560 aaaaaacaaa aacatgtaag tttattatat atttgtagta aaataatact tattttttta 1620 atattttcta acatagtttt aagtattata tattgggtta gttttaataa aacaataatt 1680 ttagatagta atattactaa tgataataat gatataaatg gagtatcatg gaatacttat 1740 tttaataatt cttttaatac actagctaca tgtggaaaag caggtaactt ttgtgaatgt 1800 tctaattata gtacatcact atataatata acaaataatt gtagcttaac tatttttcct 1860 cataatactg atatatttaa tacaacatat caactagtat atctaaatca aaaaattaat 1920 tatacaataa aattattaac acctgtagat tctccaaata tcacatataa ttgtactaat 1980 tctttaataa catgtaaaaa aaataatgga acaaacacta atatatattt aaatataaat 2040 gatactatag ttaattatac taatgaaagt atacttaatt attattggaa taatagtaac 2100 attaacaatt ttacagctac atgtataatt aataatacaa ttagttcttc taatgaaaca 2160 gaacttataa attgtactaa tcccacattg aaatatcaaa actattttta tacttttttt 2220 aaattatttt atattataat atttatcgta tctgggataa taataagtat tctttttata 2280 tccatcataa cttttttatc tttacgaaaa agaaaaaaac atgttgaaga aatagaaagt 2340 ccaccacctt ctgaatctaa tgaagaagaa caatgtcagc atgatgacac cacttccata 2400 catgaaccat ctcccagaga accattactt cctaagcctt acagtcgtta tcagtataat 2460 acacctattt actacatgcg tccctcaaca caaccactca acccatttcc cttacctaaa 2520 ccgtgtcctc cacccaaacc atgtccgcca cccaaaccat gtcctccacc taaaccatgt 2580 ccttcagctg aatcctattc tccacccaaa ccactaccta gtatcccgct actacccaat 2640 atcccgccat tatctaccca aaatatttcg cttattcacg tagatagaat tatttatcat 2700 gattatgctt caaagctgtt agccgattat aggtcagatc cacctctttg ggaaagcgat 2760 ttgccgcgtc ataaccggta ttctgataat atactaaatt cccggtactg cggcaacaaa 2820 aatggagctg cgcctgtata caatgaatac actaatagtc ctgaaaaggc tgaaaaggga 2880 ttgcaactca gtgatctacg taacttttct tttatgctga accctcagca caaaaacatt 2940 ggttacggcg atgcgcaaga tctggagccg tacagctcca ttccaaaaaa taaacttttt 3000 aatcatttta aaaatcatcg tcctgctttt tcaacccata cggaaaacct aattaggcgc 3060 aatgttgttc gtacggaaaa aaaaacattt ccgcaggtgg ctagcttaaa gggcacacag 3120 aaaaattgtc tgacccagcc ttcctcattg ccttccttaa aaaacccaaa aaatagttcg 3180 gttccttcta cgaggttcag cgagcacaca aaattttttt cgtacgagga tcttcctaaa 3240 ctcagaacaa agggtaccat taagcatgag cagcaccttg gcgatcagat gccgggccag 3300 cactataacg gatatattcc gcacaaagac gtgtacaata ttttgtgttt agcgcacaat 3360 ctacctgcgt cggtagaaaa agggattgca ggtagaggta ttcctttagg aaatccacat 3420 gtcaaaccga acatagagca ggaactcata aaatccacca gtacctacac ggacgtgccg 3480 atgcttggtc cccttccgcc caaagattcg caacacggca gagaatacca ggaattttcc 3540 gcaaatagac atatgctaca agtatctaat atactacatt ctgtgtttgc taatcattct 3600 ataaagccac aaatcctaga agacataccg gtactcaatg cacaactaac aagcataaag 3660 ccggtaagcc catttttaaa caaagcgtat caaacccatt atatggaaaa tattgttacc 3720 ttggtgcccc ggtttaagag tattgccaac tactcaagcc ctataccgaa ttattctaaa 3780 agaaatagcg gtcaggctga atattttgat acctcaaagc aaacaatttc tagacacaat 3840 aattatatac ctaaatatac gggaggaatt ggagattcca aactagacag cacctttcct 3900 aaagatttca acgcctcctc ggtaccgtta acaagcgcgg aaaaggacca ttccctgcgg 3960 ggagacaact cagcatgttg catctcatct atatctccat cattgccctc taatatgaaa 4020 cagttttgca agatttctgt atggctacag cagcacgatc cagatttatt agaaattatc 4080 aacaacttat gtatgcttgg caatttatcc gcggcaaagt acaaacacgg agttaccttc 4140 atttacccca aacaggcaaa gatccgcgat gaaataaaaa aacatgccta ctccaatgac 4200 ccttcacaag ccataaagac cttagaatca ctcatccttc cattttacat tcccactcca 4260 gcggagttca ccggggaaat cggctcctac accggagtga aattagaggt tgaaaaaacg 4320 gaggcgaata aagttatttt aaaaaatgga gaagcggtcc tagtaccggc ggccgatttt 4380 aagccctttc ctgatcgccg actagcggtc tggatcatgg agtcaggctc tatgcccctg 4440 gagggtcccc cctataagcg gaaaaaggag ggtgggggga atgacccgcc ggttcctaag 4500 catatctcgc cgtatactcc gcgcacgcgt attgccattg aggtggaaaa ggcctttgat 4560 gactgtatgc gtcaaaactg gtgtagtgtc aataatccct atcttgccaa gtcggtctcc 4620 ttgctgtctt tcttgtcgct caaccatccc accgagttta ttaaggtact gccgcttata 4680 gactttgacc ccttggtgac cttttatcta cttcttgagc cctataaaac gcatggggat 4740 gactttttaa ttccggaaac cattttattc ggccctaccg gatggaatgg tacagatctg 4800 tatcaaagtg ccatgctgga gtttaaaaag ttttttaccc agattactcg ccaaaccttt 4860 atggacatag ccgattcggc tactaaggag gtagatgttc ccatatgtta ctcggatccc 4920 gaaaccgtac attcctatgc caatcacgtg cgtactgaaa ttttgcatca caatgccgtc 4980 aataaggtta caacacctaa cctcgtcgtg caggcctata atgagctcga gcaaaccaat 5040 accatacgac attacggccc tattttcccg gaaagtacca tcaacgcact gcgtttttgg 5100 aaaaagctgt ggcaggatga acagcgattt gttatccacg gcctgcaccg cacgttgatg 5160 gatcaaccca cctatgaaac ctctgagttt gcagagatcg ttagaaattt acggttttcg 5220 cgtcccggca ataactatat aaacgagctt aatattacaa gtcccgctat gtacggcgac 5280 aagcatacca ccggagatat tgcgcccaat gatagatttg ccatgttggt ggcctttatc 5340 aacagtactg actttttata caccgcgatt cccgaggaaa aggtaggggg gaatgaaacc 5400 caaaccagta gccttacaga cctagttcca acacggctac actctttttt aaatcataat 5460 ctaagcaaac ttaaaatctt aaaccgcgcg cagcaaacgg ttagaaatat tctttcaaat 5520 gattgtctta atcaactgaa acattatgtt aaacacacgg gaaaaaatga aatactaaag 5580 ttacttcaag aatattttct agtagcagat catcgagaac atcatgtgat tccttttctt 5640 aaaaccgatt tccatcacat gcatcaaaat cctatacaaa aaaaacaagc tctcctagaa 5700 atcaaacagc tttttactgg agattatctc atctgcaaaa gcccttctac cattctggcc 5760 tgtattgaac gaaaaaccta caaagacttt gcggcttctt tgaaagatgg acgttataaa 5820 aatcgccaaa aaatgctgtc gctgcgagaa caaaccaact gtcaacttta tttttttgta 5880 gaaggcccgg catttcctaa ccctcaaaaa aaaattaatc acgttgccta tgcaagcatt 5940 attactgcta tgacgcatct tatggttaga gatcatattt ttgtcattca aacgaaaaat 6000 gaggcccaca gttcccaaaa gcttgtgcag cttttttatg ccttttctaa ggaaatggtg 6060 tgcgtcgttc ccacctccct cacccccacg gatgaagagc tatgcatcaa gctatggtct 6120 tctctttctg gtatttcagg cgtgataggt aaaatcttgg caaacacttg ttccgtagct 6180 catttggttt ctggaaagct ttcatcgcag aatattgatc agttaaaaac tccctccaac 6240 cgaccattcc ccaaaaaagt aaaacgtatg cttataagca ttagcaaagg aaataaggag 6300 ttagaaataa aattgctctc gggggttccc aatatcggga aaaaattagc tgccgaaatt 6360 ttaaaagatc atgcgcttct tttttttcta aatcagcccg tagaatgctt ggcaaatata 6420 caaatcgttc aaaaaacccg tacgattaag ttgggaatga agcgagccga agcgattcat 6480 tattttttaa actggtgtgg ctctgcccat gtaaccgatg atagccaaaa tatcacagag 6540 gcgtcgcggc ctacaatgca ggtcgcgacg cagtccgccg caactcagcc cgctgcaacg 6600 cagccattgc acgaagtatc agatgatgca tcatcagatg cttcatcacc cgtagggcat 6660 caaacattat ctaaagaaat gttattgaac acagccgttg agacacaaat ggacaaactt 6720 ggttttctgc taaatcacat aggtaaacag gttaccacta aggtgcttag caatgcccat 6780 ataactcaaa cgatgaagga gattattttg gaaaatcata gtgtagatgg tggagccgca 6840 aaaaatgttt caaaagggaa gtcttcccca aaagaaaaaa aacattggac tgagttcgaa 6900 tcctgggaac agctcagcaa gtctaaaaga agttttaagg aatactgggc ggagcgtaat 6960 gagattgtga atactctgtt gcttaactgg gacaatgttc ggggtgccat caaaaaattt 7020 ttggacgatg accgtgaatg gtgcggccgc attaatatga taaacggtgt acccgagata 7080 gtggaaatca ttccaagccc ctatagggca ggagagaaca tttattttgg cagcgaggct 7140 atgatgcctg ctgatattta tagcagggtg gccaacaagc ctgctatgtt tgtgtttcac 7200 acgcatccta atttgggttc atgttgtgga ggaatgcctt ccatatgtga catttctaca 7260 acgctgcgtt atctactcat ggggtggact gctgggcatc taatcatttc ttcgaatcaa 7320 gtaggaatgc tcacggttga taagagaatt attgttgatt tgtgggccaa tgagaatccg 7380 cgctggctta tggcgcaaaa aatattagat atttttatga tgctcacttc gcgtagaagc 7440 ctggtaaatc cctggaccct gagagaccta aaaaaaatat tacaagacta tggtattgag 7500 tatatcattt ttccttcgaa tgactttttt atttatgaag acgaacgtct tttaatgttt 7560 tcaaaaaaat ggaccaactt ttttacgtta catgagttat tggatgacct cgaaactatt 7620 gagacaaagg catcgtccac aacatcgaca aaaaaaaagc ccacaattac caagcaagag 7680 ctttactcct tagtagcggc agatacccag ttaaataaag cattgattga aagaatcttt 7740 acaagtcagc aaaaaataat acaaaatgct ttaaagcaca atcaagaagt tattatacca 7800 cccggaatca agttcaccgt cgttacggtg aaagctaaac ctgctcgcca gggccataat 7860 cccgccacag gagagcctat tcaaattaaa gctaaacctg aacataaagc cgtaaagata 7920 cgagcattga aacctgtcca tgatatgtta aacgttgagc cacgcgaaca gttttttcaa 7980 gatctgcttt cagcagtgga tcaacaaatg gacactgtaa aaaatgacat aaaagacatt 8040 atgaaagaaa aaacgtcttt tatggtatca ttcgaaaact ttatagaacg ttacgatacc 8100 atggaaaaaa atattcaaga ccttcagaat aagtacgaag aaatggcggc caaccttatg 8160 accgtcatga cggatacaaa aattcagctt ggagccatta tcgcccaact tgagattcta 8220 atgataaatg gcactccact tccggcaaaa aagacaacaa ttaaggaggc tatgccctta 8280 ccttcatcaa acacgaataa tgaacaaacg agtcctcccg cctcaggcaa aacaagtgaa 8340 acacctaaaa aaaatcccac gaatgcgatg ttcttcacgc gtagcgaatg ggcatcctcg 8400 aatacttttc gagaaaagtt tttaacacca gaaattcaag ccatattgga tgagcagttt 8460 gcaaacaaga ccgggatcga aagattgcat gccgagggtc tttacatgtg gagaacccaa 8520 ttctctgacg aacagaagaa aatggtcaaa gagatgatga agaag 8565 <210> 32 <211> 2694 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 2 <400> 32 gattttattt taaatatatc catgaaaatg gaggtcatct tcaaaacgga tttaagatca 60 tcttcacaag ttgtgtttca tgcgggtagc ctgtataatt ggttttctgt tgagattatc 120 aatagcggta gaattgttac gaccgctata aaaacattgc ttagtactgt taagtatgat 180 attgtgaaat ctgctcgtat atatgcaggg caagggtata ctgaacatca ggctcaagaa 240 gaatggaata tgattctgca tgtgctgttt gaagaggaga cggaatcctc agcatcttcg 300 gagtcaattc atgaaaaaaa tgataatgaa accaatgaat gcacatcctc ctttgaaacg 360 ttgtttgagc aagagccctc atcggagaca cctaaagact ccaagctgta tatgcttgca 420 caaaagactg tgcaacatat tgaacaatat ggaaaggcac ctgattttaa caaggttatt 480 agagcacata attttattca aaccattcat ggaacccctc taaaggaaga agaaaaagag 540 gtggtaagac tcatggttat taaactttta aaaaaaaaag attctgaatt ttttcaaccg 600 gtttatccgc ggcattatgg tgagtgtttg tcaccagtca ctacaccaag cttcttctcc 660 acacatatgt atactattct cattgctatc gtggtcttag tcatcattat catcgttcta 720 atctatctat tctcttcaag aaagaaaaaa gctgctgctg ctattgagga ggaagatata 780 cagtttataa atccttatca agatcagcag tgggcagaag tcactccaca accaggtacc 840 tctaaaccag ctggagcgac tacagcaagt gtaggcaagc cagtcacggg cagaccggca 900 acaaacagac cagcaacaaa caaaccagtt acggacaacc cagttacgga cagactagtc 960 atggcaactg gcgggccggc ggccgcacct gcggccgcga gtgctcctgc tcatccggct 1020 gagccttaca cgacagtcac tactcagaac actgcttcac aaacaatgtc ggctattgaa 1080 aatttacgac aaagaaacac ctatacgcat aaagacctag aaaactcctt gttttctaag 1140 aaaaagtaca tcggtcttat cgataagaag tgtgaattga aaaaaatact agatgattat 1200 agtatattaa taattggaat attaattgga actaacatca taacccttat ttataatttt 1260 ataggatgga ttaattatat aaaatgttac caaaatgatg ataagatgtt ttattgccct 1320 aaagattggg ttggatataa taatgtttgt tattatttta gtaatgaaga aaaaaattat 1380 actaatgcaa gtaattattg taagcaatta aatagtacgc ttgctaataa taatactaat 1440 ttagtaaatc ttactaaaac attaaatctt actaaaacat ataatcacga atctaattat 1500 tgggttaatt attctttaat taaaaatgag tcagtactat tacgtaatag tggatattac 1560 aaaaaacaaa aacatgtaag tttattatat atttgtagta aaataatact tattttttta 1620 atattttcta acatagtttt aagtattata tattgggtta gttttaataa aacaataatt 1680 ttagatagta atattactaa tgataataat gatataaatg gagtatcatg gaatacttat 1740 tttaataatt cttttaatac actagctaca tgtggaaaag caggtaactt ttgtgaatgt 1800 tctaattata gtacatcact atataatata acaaataatt gtagcttaac tatttttcct 1860 cataatactg atatatttaa tacaacatat caactagtat atctaaatca aaaaattaat 1920 tatacaataa aattattaac acctgtagat tctccaaata tcacatataa ttgtactaat 1980 tctttaataa catgtaaaaa aaataatgga acaaacacta atatatattt aaatataaat 2040 gatactatag ttaattatac taatgaaagt atacttaatt attattggaa taatagtaac 2100 attaacaatt ttacagctac atgtataatt aataatacaa ttagttcttc taatgaaaca 2160 gaacttataa attgtactaa tcccacattg aaatatcaaa actattttta tacttttttt 2220 aaattatttt atattataat atttatcgta tctgggataa taataagtat tctttttata 2280 tccatcataa cttttttatc tttacgaaaa agaaaaaaac atgttgaaga aatagaaagt 2340 ccaccacctt ctgaatctaa tgaagaagaa caatgtcagc atgatgacac cacttccata 2400 catgaaccat ctcccagaga accattactt cctaagcctt acagtcgtta tcagtataat 2460 acacctattt actacatgcg tccctcaaca caaccactca acccatttcc cttacctaaa 2520 ccgtgtcctc cacccaaacc atgtccgcca cccaaaccat gtcctccacc taaaccatgt 2580 ccttcagctg aatcctattc tccacccaaa ccactaccta gtatcccgct actacccaat 2640 atcccgccat tatctaccca aaatatttcg cttattcacg tagatagaat tatt 2694 <210> 33 <211> 5871 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 3 <400> 33 tatcatgatt atgcttcaaa gctgttagcc gattataggt cagatccacc tctttgggaa 60 agcgatttgc cgcgtcataa ccggtattct gataatatac taaattcccg gtactgcggc 120 aacaaaaatg gagctgcgcc tgtatacaat gaatacacta atagtcctga aaaggctgaa 180 aagggattgc aactcagtga tctacgtaac ttttctttta tgctgaaccc tcagcacaaa 240 aacattggtt acggcgatgc gcaagatctg gagccgtaca gctccattcc aaaaaataaa 300 ctttttaatc attttaaaaa tcatcgtcct gctttttcaa cccatacgga aaacctaatt 360 aggcgcaatg ttgttcgtac ggaaaaaaaa acatttccgc aggtggctag cttaaagggc 420 acacagaaaa attgtctgac ccagccttcc tcattgcctt ccttaaaaaa cccaaaaaat 480 agttcggttc cttctacgag gttcagcgag cacacaaaat ttttttcgta cgaggatctt 540 cctaaactca gaacaaaggg taccattaag catgagcagc accttggcga tcagatgccg 600 ggccagcact ataacggata tattccgcac aaagacgtgt acaatatttt gtgtttagcg 660 cacaatctac ctgcgtcggt agaaaaaggg attgcaggta gaggtattcc tttaggaaat 720 ccacatgtca aaccgaacat agagcaggaa ctcataaaat ccaccagtac ctacacggac 780 gtgccgatgc ttggtcccct tccgcccaaa gattcgcaac acggcagaga ataccaggaa 840 ttttccgcaa atagacatat gctacaagta tctaatatac tacattctgt gtttgctaat 900 cattctataa agccacaaat cctagaagac ataccggtac tcaatgcaca actaacaagc 960 ataaagccgg taagcccatt tttaaacaaa gcgtatcaaa cccattatat ggaaaatatt 1020 gttaccttgg tgccccggtt taagagtatt gccaactact caagccctat accgaattat 1080 tctaaaagaa atagcggtca ggctgaatat tttgatacct caaagcaaac aatttctaga 1140 cacaataatt atatacctaa atatacggga ggaattggag attccaaact agacagcacc 1200 tttcctaaag atttcaacgc ctcctcggta ccgttaacaa gcgcggaaaa ggaccattcc 1260 ctgcggggag acaactcagc atgttgcatc tcatctatat ctccatcatt gccctctaat 1320 atgaaacagt tttgcaagat ttctgtatgg ctacagcagc acgatccaga tttattagaa 1380 attatcaaca acttatgtat gcttggcaat ttatccgcgg caaagtacaa acacggagtt 1440 accttcattt accccaaaca ggcaaagatc cgcgatgaaa taaaaaaaca tgcctactcc 1500 aatgaccctt cacaagccat aaagacctta gaatcactca tccttccatt ttacattccc 1560 actccagcgg agttcaccgg ggaaatcggc tcctacaccg gagtgaaatt agaggttgaa 1620 aaaacggagg cgaataaagt tattttaaaa aatggagaag cggtcctagt accggcggcc 1680 gattttaagc cctttcctga tcgccgacta gcggtctgga tcatggagtc aggctctatg 1740 cccctggagg gtccccccta taagcggaaa aaggagggtg gggggaatga cccgccggtt 1800 cctaagcata tctcgccgta tactccgcgc acgcgtattg ccattgaggt ggaaaaggcc 1860 tttgatgact gtatgcgtca aaactggtgt agtgtcaata atccctatct tgccaagtcg 1920 gtctccttgc tgtctttctt gtcgctcaac catcccaccg agtttattaa ggtactgccg 1980 cttatagact ttgacccctt ggtgaccttt tatctacttc ttgagcccta taaaacgcat 2040 ggggatgact ttttaattcc ggaaaccatt ttattcggcc ctaccggatg gaatggtaca 2100 gatctgtatc aaagtgccat gctggagttt aaaaagtttt ttacccagat tactcgccaa 2160 acctttatgg acatagccga ttcggctact aaggaggtag atgttcccat atgttactcg 2220 gatcccgaaa ccgtacattc ctatgccaat cacgtgcgta ctgaaatttt gcatcacaat 2280 gccgtcaata aggttacaac acctaacctc gtcgtgcagg cctataatga gctcgagcaa 2340 accaatacca tacgacatta cggccctatt ttcccggaaa gtaccatcaa cgcactgcgt 2400 ttttggaaaa agctgtggca ggatgaacag cgatttgtta tccacggcct gcaccgcacg 2460 ttgatggatc aacccaccta tgaaacctct gagtttgcag agatcgttag aaatttacgg 2520 ttttcgcgtc ccggcaataa ctatataaac gagcttaata ttacaagtcc cgctatgtac 2580 ggcgacaagc ataccaccgg agatattgcg cccaatgata gatttgccat gttggtggcc 2640 tttatcaaca gtactgactt tttatacacc gcgattcccg aggaaaaggt aggggggaat 2700 gaaacccaaa ccagtagcct tacagaccta gttccaacac ggctacactc ttttttaaat 2760 cataatctaa gcaaacttaa aatcttaaac cgcgcgcagc aaacggttag aaatattctt 2820 tcaaatgatt gtcttaatca actgaaacat tatgttaaac acacgggaaa aaatgaaata 2880 ctaaagttac ttcaagaata ttttctagta gcagatcatc gagaacatca tgtgattcct 2940 tttcttaaaa ccgatttcca tcacatgcat caaaatccta tacaaaaaaa acaagctctc 3000 ctagaaatca aacagctttt tactggagat tatctcatct gcaaaagccc ttctaccatt 3060 ctggcctgta ttgaacgaaa aacctacaaa gactttgcgg cttctttgaa agatggacgt 3120 tataaaaatc gccaaaaaat gctgtcgctg cgagaacaaa ccaactgtca actttatttt 3180 tttgtagaag gcccggcatt tcctaaccct caaaaaaaaa ttaatcacgt tgcctatgca 3240 agcattatta ctgctatgac gcatcttatg gttagagatc atatttttgt cattcaaacg 3300 aaaaatgagg cccacagttc ccaaaagctt gtgcagcttt tttatgcctt ttctaaggaa 3360 atggtgtgcg tcgttcccac ctccctcacc cccacggatg aagagctatg catcaagcta 3420 tggtcttctc tttctggtat ttcaggcgtg ataggtaaaa tcttggcaaa cacttgttcc 3480 gtagctcatt tggtttctgg aaagctttca tcgcagaata ttgatcagtt aaaaactccc 3540 tccaaccgac cattccccaa aaaagtaaaa cgtatgctta taagcattag caaaggaaat 3600 aaggagttag aaataaaatt gctctcgggg gttcccaata tcgggaaaaa attagctgcc 3660 gaaattttaa aagatcatgc gcttcttttt tttctaaatc agcccgtaga atgcttggca 3720 aatatacaaa tcgttcaaaa aacccgtacg attaagttgg gaatgaagcg agccgaagcg 3780 attcattatt ttttaaactg gtgtggctct gcccatgtaa ccgatgatag ccaaaatatc 3840 acagaggcgt cgcggcctac aatgcaggtc gcgacgcagt ccgccgcaac tcagcccgct 3900 gcaacgcagc cattgcacga agtatcagat gatgcatcat cagatgcttc atcacccgta 3960 gggcatcaaa cattatctaa agaaatgtta ttgaacacag ccgttgagac acaaatggac 4020 aaacttggtt ttctgctaaa tcacataggt aaacaggtta ccactaaggt gcttagcaat 4080 gcccatataa ctcaaacgat gaaggagatt attttggaaa atcatagtgt agatggtgga 4140 gccgcaaaaa atgtttcaaa agggaagtct tccccaaaag aaaaaaaaca ttggactgag 4200 ttcgaatcct gggaacagct cagcaagtct aaaagaagtt ttaaggaata ctgggcggag 4260 cgtaatgaga ttgtgaatac tctgttgctt aactgggaca atgttcgggg tgccatcaaa 4320 aaatttttgg acgatgaccg tgaatggtgc ggccgcatta atatgataaa cggtgtaccc 4380 gagatagtgg aaatcattcc aagcccctat agggcaggag agaacattta ttttggcagc 4440 gaggctatga tgcctgctga tatttatagc agggtggcca acaagcctgc tatgtttgtg 4500 tttcacacgc atcctaattt gggttcatgt tgtggaggaa tgccttccat atgtgacatt 4560 tctacaacgc tgcgttatct actcatgggg tggactgctg ggcatctaat catttcttcg 4620 aatcaagtag gaatgctcac ggttgataag agaattattg ttgatttgtg ggccaatgag 4680 aatccgcgct ggcttatggc gcaaaaaata ttagatattt ttatgatgct cacttcgcgt 4740 agaagcctgg taaatccctg gaccctgaga gacctaaaaa aaatattaca agactatggt 4800 attgagtata tcatttttcc ttcgaatgac ttttttattt atgaagacga acgtctttta 4860 atgttttcaa aaaaatggac caactttttt acgttacatg agttattgga tgacctcgaa 4920 actattgaga caaaggcatc gtccacaaca tcgacaaaaa aaaagcccac aattaccaag 4980 caagagcttt actccttagt agcggcagat acccagttaa ataaagcatt gattgaaaga 5040 atctttacaa gtcagcaaaa aataatacaa aatgctttaa agcacaatca agaagttatt 5100 ataccacccg gaatcaagtt caccgtcgtt acggtgaaag ctaaacctgc tcgccagggc 5160 cataatcccg ccacaggaga gcctattcaa attaaagcta aacctgaaca taaagccgta 5220 aagatacgag cattgaaacc tgtccatgat atgttaaacg ttgagccacg cgaacagttt 5280 tttcaagatc tgctttcagc agtggatcaa caaatggaca ctgtaaaaaa tgacataaaa 5340 gacattatga aagaaaaaac gtcttttatg gtatcattcg aaaactttat agaacgttac 5400 gataccatgg aaaaaaatat tcaagacctt cagaataagt acgaagaaat ggcggccaac 5460 cttatgaccg tcatgacgga tacaaaaatt cagcttggag ccattatcgc ccaacttgag 5520 attctaatga taaatggcac tccacttccg gcaaaaaaga caacaattaa ggaggctatg 5580 cccttacctt catcaaacac gaataatgaa caaacgagtc ctcccgcctc aggcaaaaca 5640 agtgaaacac ctaaaaaaaa tcccacgaat gcgatgttct tcacgcgtag cgaatgggca 5700 tcctcgaata cttttcgaga aaagttttta acaccagaaa ttcaagccat attggatgag 5760 cagtttgcaa acaagaccgg gatcgaaaga ttgcatgccg agggtcttta catgtggaga 5820 acccaattct ctgacgaaca gaagaaaatg gtcaaagaga tgatgaagaa g 5871 <210> 34 <211> 4281 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 4 <400> 34 gattttattt taaatatatc catgaaaatg gaggtcatct tcaaaacgga tttaagatca 60 tcttcacaag ttgtgtttca tgcgggtagc ctgtataatt ggttttctgt tgagattatc 120 aatagcggta gaattgttac gaccgctata aaaacattgc ttagtactgt taagtatgat 180 attgtgaaat ctgctcgtat atatgcaggg caagggtata ctgaacatca ggctcaagaa 240 gaatggaata tgattctgca tgtgctgttt gaagaggaga cggaatcctc agcatcttcg 300 gagtcaattc atgaaaaaaa tgataatgaa accaatgaat gcacatcctc ctttgaaacg 360 ttgtttgagc aagagccctc atcggagaca cctaaagact ccaagctgta tatgcttgca 420 caaaagactg tgcaacatat tgaacaatat ggaaaggcac ctgattttaa caaggttatt 480 agagcacata attttattca aaccattcat ggaacccctc taaaggaaga agaaaaagag 540 gtggtaagac tcatggttat taaactttta aaaaaaaaag attctgaatt ttttcaaccg 600 gtttatccgc ggcattatgg tgagtgtttg tcaccagtca ctacaccaag cttcttctcc 660 acacatatgt atactattct cattgctatc gtggtcttag tcatcattat catcgttcta 720 atctatctat tctcttcaag aaagaaaaaa gctgctgctg ctattgagga ggaagatata 780 cagtttataa atccttatca agatcagcag tgggcagaag tcactccaca accaggtacc 840 tctaaaccag ctggagcgac tacagcaagt gtaggcaagc cagtcacggg cagaccggca 900 acaaacagac cagcaacaaa caaaccagtt acggacaacc cagttacgga cagactagtc 960 atggcaactg gcgggccggc ggccgcacct gcggccgcga gtgctcctgc tcatccggct 1020 gagccttaca cgacagtcac tactcagaac actgcttcac aaacaatgtc ggctattgaa 1080 aatttacgac aaagaaacac ctatacgcat aaagacctag aaaactcctt gccctctaat 1140 atgaaacagt tttgcaagat ttctgtatgg ctacagcagc acgatccaga tttattagaa 1200 attatcaaca acttatgtat gcttggcaat ttatccgcgg caaagtacaa acacggagtt 1260 accttcattt accccaaaca ggcaaagatc cgcgatgaaa taaaaaaaca tgcctactcc 1320 aatgaccctt cacaagccat aaagacctta gaatcactca tccttccatt ttacattccc 1380 actccagcgg agttcaccgg ggaaatcggc tcctacaccg gagtgaaatt agaggttgaa 1440 aaaacggagg cgaataaagt tattttaaaa aatggagaag cggtcctagt accggcggcc 1500 gattttaagc cctttcctga tcgccgacta gcggtctgga tcatggagtc aggctctatg 1560 cccctggagg gtccccccta taagcggaaa aaggagggtg gggggaatga cccgccggtt 1620 cctaagcata tctcgccgta tactccgcgc acgcgtattg ccattgaggt ggaaaaggcc 1680 tttgatgact gtatgcgtca aaactggtgt agtgtcaata atccctatct tgccaagtcg 1740 gtctccttgc tgtctttctt gtcgctcaac catcccaccg agtttattaa ggtactgccg 1800 cttatagact ttgacccctt ggtgaccttt tatctacttc ttgagcccta taaaacgcat 1860 ggggatgact ttttaattcc ggaaaccatt ttattcggcc ctaccggatg gaatggtaca 1920 gatctgtatc aaagtgccat gctggagttt aaaaagtttt ttacccagat tactcgccaa 1980 acctttatgg acatagccga ttcggctact aaggaggtag atgttcccat atgttactcg 2040 gatcccgaaa ccgtacattc ctatgccaat cacgtgcgta ctgaaatttt gcatcacaat 2100 gccgtcaata aggttacaac acctaacctc gtcgtgcagg cctataatga gctcgagcaa 2160 accaatacca tacgacatta cggccctatt ttcccggaaa gtaccatcaa cgcactgcgt 2220 ttttggaaaa agctgtggca ggatgaacag cgatttgtta tccacggcct gcaccgcacg 2280 ttgatggatc aacccaccta tgaaacctct gagtttgcag agatcgttag aaatttacgg 2340 ttttcgcgtc ccggcaataa ctatataaac gagcttaata ttacaagtcc cgctatgtac 2400 ggcgacaagc ataccaccgg agatattgcg cccaatgata gatttgccat gttggtggcc 2460 tttatcaaca gtactgactt tttatacacc gcgattcccg aggaaaaggt aggggggaat 2520 gaaacccaaa ccagtagcct tacagaccta gttccaacac ggctacactc ttttttaaat 2580 cataatctaa gcaaacttaa aatcttaaac cgcgcgcagc aaacggttag aaatattctt 2640 tcaaatgatt gtcttaatca actgaaacat tatgttaaac acacgggaaa aaatgaaata 2700 ctaaagttac ttcaagaatt ttctaagaaa aagtacatcg gtcttatcga taagaagtgt 2760 gaattgaaaa aaatactaga tgattatagt atattaataa ttggaatatt aattggaact 2820 aacatcataa cccttattta taattttata ggatggatta attatataaa atgttaccaa 2880 aatgatgata agatgtttta ttgccctaaa gattgggttg gatataataa tgtttgttat 2940 tattttagta atgaagaaaa aaattatact aatgcaagta attattgtaa gcaattaaat 3000 agtacgcttg ctaataataa tactaattta gtaaatctta ctaaaacatt aaatcttact 3060 aaaacatata atcacgaatc taattattgg gttaattatt ctttaattaa aaatgagtca 3120 gtactattac gtaatagtgg atattacaaa aaacaaaaac atgtaagttt attatatatt 3180 tgtagtaaaa taatacttat ttttttaata ttttctaaca tagttttaag tattatatat 3240 tgggttagtt ttaataaaac aataatttta gatagtaata ttactaatga taataatgat 3300 ataaatggag tatcatggaa tacttatttt aataattctt ttaatacact agctacatgt 3360 ggaaaagcag gtaacttttg tgaatgttct aattatagta catcactata taatataaca 3420 aataattgta gcttaactat ttttcctcat aatactgata tatttaatac aacatatcaa 3480 ctagtatatc taaatcaaaa aattaattat acaataaaat tattaacacc tgtagattct 3540 ccaaatatca catataattg tactaattct ttaataacat gtaaaaaaaa taatggaaca 3600 aacactaata tatatttaaa tataaatgat actatagtta attatactaa tgaaagtata 3660 cttaattatt attggaataa tagtaacatt aacaatttta cagctacatg tataattaat 3720 aatacaatta gttcttctaa tgaaacagaa cttataaatt gtactaatcc cacattgaaa 3780 tatcaaaact atttttatac tttttttaaa ttattttata ttataatatt tatcgtatct 3840 gggataataa taagtattct ttttatatcc atcataactt ttttatcttt acgaaaaaga 3900 aaaaaacatg ttgaagaaat agaaagtcca ccaccttctg aatctaatga agaagaacaa 3960 tgtcagcatg atgacaccac ttccatacat gaaccatctc ccagagaacc attacttcct 4020 aagccttaca gtcgttatca gtataataca cctatttact acatgcgtcc ctcaacacaa 4080 ccactcaacc catttccctt acctaaaccg tgtcctccac ccaaaccatg tccgccaccc 4140 aaaccatgtc ctccacctaa accatgtcct tcagctgaat cctattctcc acccaaacca 4200 ctacctagta tcccgctact acccaatatc ccgccattat ctacccaaaa tatttcgctt 4260 attcacgtag atagaattat t 4281 <210> 35 <211> 4284 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 5 <400> 35 tatcatgatt atgcttcaaa gctgttagcc gattataggt cagatccacc tctttgggaa 60 agcgatttgc cgcgtcataa ccggtattct gataatatac taaattcccg gtactgcggc 120 aacaaaaatg gagctgcgcc tgtatacaat gaatacacta atagtcctga aaaggctgaa 180 aagggattgc aactcagtga tctacgtaac ttttctttta tgctgaaccc tcagcacaaa 240 aacattggtt acggcgatgc gcaagatctg gagccgtaca gctccattcc aaaaaataaa 300 ctttttaatc attttaaaaa tcatcgtcct gctttttcaa cccatacgga aaacctaatt 360 aggcgcaatg ttgttcgtac ggaaaaaaaa acatttccgc aggtggctag cttaaagggc 420 acacagaaaa attgtctgac ccagccttcc tcattgcctt ccttaaaaaa cccaaaaaat 480 agttcggttc cttctacgag gttcagcgag cacacaaaat ttttttcgta cgaggatctt 540 cctaaactca gaacaaaggg taccattaag catgagcagc accttggcga tcagatgccg 600 ggccagcact ataacggata tattccgcac aaagacgtgt acaatatttt gtgtttagcg 660 cacaatctac ctgcgtcggt agaaaaaggg attgcaggta gaggtattcc tttaggaaat 720 ccacatgtca aaccgaacat agagcaggaa ctcataaaat ccaccagtac ctacacggac 780 gtgccgatgc ttggtcccct tccgcccaaa gattcgcaac acggcagaga ataccaggaa 840 ttttccgcaa atagacatat gctacaagta tctaatatac tacattctgt gtttgctaat 900 cattctataa agccacaaat cctagaagac ataccggtac tcaatgcaca actaacaagc 960 ataaagccgg taagcccatt tttaaacaaa gcgtatcaaa cccattatat ggaaaatatt 1020 gttaccttgg tgccccggtt taagagtatt gccaactact caagccctat accgaattat 1080 tctaaaagaa atagcggtca ggctgaatat tttgatacct caaagcaaac aatttctaga 1140 cacaataatt atatacctaa atatacggga ggaattggag attccaaact agacagcacc 1200 tttcctaaag atttcaacgc ctcctcggta ccgttaacaa gcgcggaaaa ggaccattcc 1260 ctgcggggag acaactcagc atgttgcatc tcatctatat ctccatcatt gtattttcta 1320 gtagcagatc atcgagaaca tcatgtgatt ccttttctta aaaccgattt ccatcacatg 1380 catcaaaatc ctatacaaaa aaaacaagct ctcctagaaa tcaaacagct ttttactgga 1440 gattatctca tctgcaaaag cccttctacc attctggcct gtattgaacg aaaaacctac 1500 aaagactttg cggcttcttt gaaagatgga cgttataaaa atcgccaaaa aatgctgtcg 1560 ctgcgagaac aaaccaactg tcaactttat ttttttgtag aaggcccggc atttcctaac 1620 cctcaaaaaa aaattaatca cgttgcctat gcaagcatta ttactgctat gacgcatctt 1680 atggttagag atcatatttt tgtcattcaa acgaaaaatg aggcccacag ttcccaaaag 1740 cttgtgcagc ttttttatgc cttttctaag gaaatggtgt gcgtcgttcc cacctccctc 1800 acccccacgg atgaagagct atgcatcaag ctatggtctt ctctttctgg tatttcaggc 1860 gtgataggta aaatcttggc aaacacttgt tccgtagctc atttggtttc tggaaagctt 1920 tcatcgcaga atattgatca gttaaaaact ccctccaacc gaccattccc caaaaaagta 1980 aaacgtatgc ttataagcat tagcaaagga aataaggagt tagaaataaa attgctctcg 2040 ggggttccca atatcgggaa aaaattagct gccgaaattt taaaagatca tgcgcttctt 2100 ttttttctaa atcagcccgt agaatgcttg gcaaatatac aaatcgttca aaaaacccgt 2160 acgattaagt tgggaatgaa gcgagccgaa gcgattcatt attttttaaa ctggtgtggc 2220 tctgcccatg taaccgatga tagccaaaat atcacagagg cgtcgcggcc tacaatgcag 2280 gtcgcgacgc agtccgccgc aactcagccc gctgcaacgc agccattgca cgaagtatca 2340 gatgatgcat catcagatgc ttcatcaccc gtagggcatc aaacattatc taaagaaatg 2400 ttattgaaca cagccgttga gacacaaatg gacaaacttg gttttctgct aaatcacata 2460 ggtaaacagg ttaccactaa ggtgcttagc aatgcccata taactcaaac gatgaaggag 2520 attattttgg aaaatcatag tgtagatggt ggagccgcaa aaaatgtttc aaaagggaag 2580 tcttccccaa aagaaaaaaa acattggact gagttcgaat cctgggaaca gctcagcaag 2640 tctaaaagaa gttttaagga atactgggcg gagcgtaatg agattgtgaa tactctgttg 2700 cttaactggg acaatgttcg gggtgccatc aaaaaatttt tggacgatga ccgtgaatgg 2760 tgcggccgca ttaatatgat aaacggtgta cccgagatag tggaaatcat tccaagcccc 2820 tatagggcag gagagaacat ttattttggc agcgaggcta tgatgcctgc tgatatttat 2880 agcagggtgg ccaacaagcc tgctatgttt gtgtttcaca cgcatcctaa tttgggttca 2940 tgttgtggag gaatgccttc catatgtgac atttctacaa cgctgcgtta tctactcatg 3000 gggtggactg ctgggcatct aatcatttct tcgaatcaag taggaatgct cacggttgat 3060 aagagaatta ttgttgattt gtgggccaat gagaatccgc gctggcttat ggcgcaaaaa 3120 atattagata tttttatgat gctcacttcg cgtagaagcc tggtaaatcc ctggaccctg 3180 agagacctaa aaaaaatatt acaagactat ggtattgagt atatcatttt tccttcgaat 3240 gactttttta tttatgaaga cgaacgtctt ttaatgtttt caaaaaaatg gaccaacttt 3300 tttacgttac atgagttatt ggatgacctc gaaactattg agacaaaggc atcgtccaca 3360 acatcgacaa aaaaaaagcc cacaattacc aagcaagagc tttactcctt agtagcggca 3420 gatacccagt taaataaagc attgattgaa agaatcttta caagtcagca aaaaataata 3480 caaaatgctt taaagcacaa tcaagaagtt attataccac ccggaatcaa gttcaccgtc 3540 gttacggtga aagctaaacc tgctcgccag ggccataatc ccgccacagg agagcctatt 3600 caaattaaag ctaaacctga acataaagcc gtaaagatac gagcattgaa acctgtccat 3660 gatatgttaa acgttgagcc acgcgaacag ttttttcaag atctgctttc agcagtggat 3720 caacaaatgg acactgtaaa aaatgacata aaagacatta tgaaagaaaa aacgtctttt 3780 atggtatcat tcgaaaactt tatagaacgt tacgatacca tggaaaaaaa tattcaagac 3840 cttcagaata agtacgaaga aatggcggcc aaccttatga ccgtcatgac ggatacaaaa 3900 attcagcttg gagccattat cgcccaactt gagattctaa tgataaatgg cactccactt 3960 ccggcaaaaa agacaacaat taaggaggct atgcccttac cttcatcaaa cacgaataat 4020 gaacaaacga gtcctcccgc ctcaggcaaa acaagtgaaa cacctaaaaa aaatcccacg 4080 aatgcgatgt tcttcacgcg tagcgaatgg gcatcctcga atacttttcg agaaaagttt 4140 ttaacaccag aaattcaagc catattggat gagcagtttg caaacaagac cgggatcgaa 4200 agattgcatg ccgagggtct ttacatgtgg agaacccaat tctctgacga acagaagaaa 4260 atggtcaaag agatgatgaa gaag 4284 <210> 36 <211> 8790 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 1' <400> 36 cagatctttg taaagacctt gactggtaag accatcaccc tggaagtgga gcccagtgac 60 accattgaga atgtcaaggg gaagattcaa gaaaaagagg gcatcccccc agaccagcag 120 aggctgatct ttgccgggaa gcagctggaa gatgggcgca ccctgtctga ctacaacatc 180 cagaaggagt ccaccctgca cctggtcctc cgcctcagag gtgccgattt tattttaaat 240 atatccatga aaatggaggt catcttcaaa acggatttaa gatcatcttc acaagttgtg 300 tttcatgcgg gtagcctgta taattggttt tctgttgaga ttatcaatag cggtagaatt 360 gttacgaccg ctataaaaac attgcttagt actgttaagt atgatattgt gaaatctgct 420 cgtatatatg cagggcaagg gtatactgaa catcaggctc aagaagaatg gaatatgatt 480 ctgcatgtgc tgtttgaaga ggagacggaa tcctcagcat cttcggagtc aattcatgaa 540 aaaaatgata atgaaaccaa tgaatgcaca tcctcctttg aaacgttgtt tgagcaagag 600 ccctcatcgg agacacctaa agactccaag ctgtatatgc ttgcacaaaa gactgtgcaa 660 catattgaac aatatggaaa ggcacctgat tttaacaagg ttattagagc acataatttt 720 attcaaacca ttcatggaac ccctctaaag gaagaagaaa aagaggtggt aagactcatg 780 gttattaaac ttttaaaaaa aaaagattct gaattttttc aaccggttta tccgcggcat 840 tatggtgagt gtttgtcacc agtcactaca ccaagcttct tctccacaca tatgtatact 900 attctcattg ctatcgtggt cttagtcatc attatcatcg ttctaatcta tctattctct 960 tcaagaaaga aaaaagctgc tgctgctatt gaggaggaag atatacagtt tataaatcct 1020 tatcaagatc agcagtgggc agaagtcact ccacaaccag gtacctctaa accagctgga 1080 gcgactacag caagtgtagg caagccagtc acgggcagac cggcaacaaa cagaccagca 1140 acaaacaaac cagttacgga caacccagtt acggacagac tagtcatggc aactggcggg 1200 ccggcggccg cacctgcggc cgcgagtgct cctgctcatc cggctgagcc ttacacgaca 1260 gtcactactc agaacactgc ttcacaaaca atgtcggcta ttgaaaattt acgacaaaga 1320 aacacctata cgcataaaga cctagaaaac tccttgtttt ctaagaaaaa gtacatcggt 1380 cttatcgata agaagtgtga attgaaaaaa atactagatg attatagtat attaataatt 1440 ggaatattaa ttggaactaa catcataacc cttatttata attttatagg atggattaat 1500 tatataaaat gttaccaaaa tgatgataag atgttttatt gccctaaaga ttgggttgga 1560 tataataatg tttgttatta ttttagtaat gaagaaaaaa attatactaa tgcaagtaat 1620 tattgtaagc aattaaatag tacgcttgct aataataata ctaatttagt aaatcttact 1680 aaaacattaa atcttactaa aacatataat cacgaatcta attattgggt taattattct 1740 ttaattaaaa atgagtcagt actattacgt aatagtggat attacaaaaa acaaaaacat 1800 gtaagtttat tatatatttg tagtaaaata atacttattt ttttaatatt ttctaacata 1860 gttttaagta ttatatattg ggttagtttt aataaaacaa taattttaga tagtaatatt 1920 actaatgata ataatgatat aaatggagta tcatggaata cttattttaa taattctttt 1980 aatacactag ctacatgtgg aaaagcaggt aacttttgtg aatgttctaa ttatagtaca 2040 tcactatata atataacaaa taattgtagc ttaactattt ttcctcataa tactgatata 2100 tttaatacaa catatcaact agtatatcta aatcaaaaaa ttaattatac aataaaatta 2160 ttaacacctg tagattctcc aaatatcaca tataattgta ctaattcttt aataacatgt 2220 aaaaaaaata atggaacaaa cactaatata tatttaaata taaatgatac tatagttaat 2280 tatactaatg aaagtatact taattattat tggaataata gtaacattaa caattttaca 2340 gctacatgta taattaataa tacaattagt tcttctaatg aaacagaact tataaattgt 2400 actaatccca cattgaaata tcaaaactat ttttatactt tttttaaatt attttatatt 2460 ataatattta tcgtatctgg gataataata agtattcttt ttatatccat cataactttt 2520 ttatctttac gaaaaagaaa aaaacatgtt gaagaaatag aaagtccacc accttctgaa 2580 tctaatgaag aagaacaatg tcagcatgat gacaccactt ccatacatga accatctccc 2640 agagaaccat tacttcctaa gccttacagt cgttatcagt ataatacacc tatttactac 2700 atgcgtccct caacacaacc actcaaccca tttcccttac ctaaaccgtg tcctccaccc 2760 aaaccatgtc cgccacccaa accatgtcct ccacctaaac catgtccttc agctgaatcc 2820 tattctccac ccaaaccact acctagtatc ccgctactac ccaatatccc gccattatct 2880 acccaaaata tttcgcttat tcacgtagat agaattattt atcatgatta tgcttcaaag 2940 ctgttagccg attataggtc agatccacct ctttgggaaa gcgatttgcc gcgtcataac 3000 cggtattctg ataatatact aaattcccgg tactgcggca acaaaaatgg agctgcgcct 3060 gtatacaatg aatacactaa tagtcctgaa aaggctgaaa agggattgca actcagtgat 3120 ctacgtaact tttcttttat gctgaaccct cagcacaaaa acattggtta cggcgatgcg 3180 caagatctgg agccgtacag ctccattcca aaaaataaac tttttaatca ttttaaaaat 3240 catcgtcctg ctttttcaac ccatacggaa aacctaatta ggcgcaatgt tgttcgtacg 3300 gaaaaaaaaa catttccgca ggtggctagc ttaaagggca cacagaaaaa ttgtctgacc 3360 cagccttcct cattgccttc cttaaaaaac ccaaaaaata gttcggttcc ttctacgagg 3420 ttcagcgagc acacaaaatt tttttcgtac gaggatcttc ctaaactcag aacaaagggt 3480 accattaagc atgagcagca ccttggcgat cagatgccgg gccagcacta taacggatat 3540 attccgcaca aagacgtgta caatattttg tgtttagcgc acaatctacc tgcgtcggta 3600 gaaaaaggga ttgcaggtag aggtattcct ttaggaaatc cacatgtcaa accgaacata 3660 gagcaggaac tcataaaatc caccagtacc tacacggacg tgccgatgct tggtcccctt 3720 ccgcccaaag attcgcaaca cggcagagaa taccaggaat tttccgcaaa tagacatatg 3780 ctacaagtat ctaatatact acattctgtg tttgctaatc attctataaa gccacaaatc 3840 ctagaagaca taccggtact caatgcacaa ctaacaagca taaagccggt aagcccattt 3900 ttaaacaaag cgtatcaaac ccattatatg gaaaatattg ttaccttggt gccccggttt 3960 aagagtattg ccaactactc aagccctata ccgaattatt ctaaaagaaa tagcggtcag 4020 gctgaatatt ttgatacctc aaagcaaaca atttctagac acaataatta tatacctaaa 4080 tatacgggag gaattggaga ttccaaacta gacagcacct ttcctaaaga tttcaacgcc 4140 tcctcggtac cgttaacaag cgcggaaaag gaccattccc tgcggggaga caactcagca 4200 tgttgcatct catctatatc tccatcattg ccctctaata tgaaacagtt ttgcaagatt 4260 tctgtatggc tacagcagca cgatccagat ttattagaaa ttatcaacaa cttatgtatg 4320 cttggcaatt tatccgcggc aaagtacaaa cacggagtta ccttcattta ccccaaacag 4380 gcaaagatcc gcgatgaaat aaaaaaacat gcctactcca atgacccttc acaagccata 4440 aagaccttag aatcactcat ccttccattt tacattccca ctccagcgga gttcaccggg 4500 gaaatcggct cctacaccgg agtgaaatta gaggttgaaa aaacggaggc gaataaagtt 4560 attttaaaaa atggagaagc ggtcctagta ccggcggccg attttaagcc ctttcctgat 4620 cgccgactag cggtctggat catggagtca ggctctatgc ccctggaggg tcccccctat 4680 aagcggaaaa aggagggtgg ggggaatgac ccgccggttc ctaagcatat ctcgccgtat 4740 actccgcgca cgcgtattgc cattgaggtg gaaaaggcct ttgatgactg tatgcgtcaa 4800 aactggtgta gtgtcaataa tccctatctt gccaagtcgg tctccttgct gtctttcttg 4860 tcgctcaacc atcccaccga gtttattaag gtactgccgc ttatagactt tgaccccttg 4920 gtgacctttt atctacttct tgagccctat aaaacgcatg gggatgactt tttaattccg 4980 gaaaccattt tattcggccc taccggatgg aatggtacag atctgtatca aagtgccatg 5040 ctggagttta aaaagttttt tacccagatt actcgccaaa cctttatgga catagccgat 5100 tcggctacta aggaggtaga tgttcccata tgttactcgg atcccgaaac cgtacattcc 5160 tatgccaatc acgtgcgtac tgaaattttg catcacaatg ccgtcaataa ggttacaaca 5220 cctaacctcg tcgtgcaggc ctataatgag ctcgagcaaa ccaataccat acgacattac 5280 ggccctattt tcccggaaag taccatcaac gcactgcgtt tttggaaaaa gctgtggcag 5340 gatgaacagc gatttgttat ccacggcctg caccgcacgt tgatggatca acccacctat 5400 gaaacctctg agtttgcaga gatcgttaga aatttacggt tttcgcgtcc cggcaataac 5460 tatataaacg agcttaatat tacaagtccc gctatgtacg gcgacaagca taccaccgga 5520 gatattgcgc ccaatgatag atttgccatg ttggtggcct ttatcaacag tactgacttt 5580 ttatacaccg cgattcccga ggaaaaggta ggggggaatg aaacccaaac cagtagcctt 5640 acagacctag ttccaacacg gctacactct tttttaaatc ataatctaag caaacttaaa 5700 atcttaaacc gcgcgcagca aacggttaga aatattcttt caaatgattg tcttaatcaa 5760 ctgaaacatt atgttaaaca cacgggaaaa aatgaaatac taaagttact tcaagaatat 5820 tttctagtag cagatcatcg agaacatcat gtgattcctt ttcttaaaac cgatttccat 5880 cacatgcatc aaaatcctat acaaaaaaaa caagctctcc tagaaatcaa acagcttttt 5940 actggagatt atctcatctg caaaagccct tctaccattc tggcctgtat tgaacgaaaa 6000 acctacaaag actttgcggc ttctttgaaa gatggacgtt ataaaaatcg ccaaaaaatg 6060 ctgtcgctgc gagaacaaac caactgtcaa ctttattttt ttgtagaagg cccggcattt 6120 cctaaccctc aaaaaaaaat taatcacgtt gcctatgcaa gcattattac tgctatgacg 6180 catcttatgg ttagagatca tatttttgtc attcaaacga aaaatgaggc ccacagttcc 6240 caaaagcttg tgcagctttt ttatgccttt tctaaggaaa tggtgtgcgt cgttcccacc 6300 tccctcaccc ccacggatga agagctatgc atcaagctat ggtcttctct ttctggtatt 6360 tcaggcgtga taggtaaaat cttggcaaac acttgttccg tagctcattt ggtttctgga 6420 aagctttcat cgcagaatat tgatcagtta aaaactccct ccaaccgacc attccccaaa 6480 aaagtaaaac gtatgcttat aagcattagc aaaggaaata aggagttaga aataaaattg 6540 ctctcggggg ttcccaatat cgggaaaaaa ttagctgccg aaattttaaa agatcatgcg 6600 cttctttttt ttctaaatca gcccgtagaa tgcttggcaa atatacaaat cgttcaaaaa 6660 acccgtacga ttaagttggg aatgaagcga gccgaagcga ttcattattt tttaaactgg 6720 tgtggctctg cccatgtaac cgatgatagc caaaatatca cagaggcgtc gcggcctaca 6780 atgcaggtcg cgacgcagtc cgccgcaact cagcccgctg caacgcagcc attgcacgaa 6840 gtatcagatg atgcatcatc agatgcttca tcacccgtag ggcatcaaac attatctaaa 6900 gaaatgttat tgaacacagc cgttgagaca caaatggaca aacttggttt tctgctaaat 6960 cacataggta aacaggttac cactaaggtg cttagcaatg cccatataac tcaaacgatg 7020 aaggagatta ttttggaaaa tcatagtgta gatggtggag ccgcaaaaaa tgtttcaaaa 7080 gggaagtctt ccccaaaaga aaaaaaacat tggactgagt tcgaatcctg ggaacagctc 7140 agcaagtcta aaagaagttt taaggaatac tgggcggagc gtaatgagat tgtgaatact 7200 ctgttgctta actgggacaa tgttcggggt gccatcaaaa aatttttgga cgatgaccgt 7260 gaatggtgcg gccgcattaa tatgataaac ggtgtacccg agatagtgga aatcattcca 7320 agcccctata gggcaggaga gaacatttat tttggcagcg aggctatgat gcctgctgat 7380 atttatagca gggtggccaa caagcctgct atgtttgtgt ttcacacgca tcctaatttg 7440 ggttcatgtt gtggaggaat gccttccata tgtgacattt ctacaacgct gcgttatcta 7500 ctcatggggt ggactgctgg gcatctaatc atttcttcga atcaagtagg aatgctcacg 7560 gttgataaga gaattattgt tgatttgtgg gccaatgaga atccgcgctg gcttatggcg 7620 caaaaaatat tagatatttt tatgatgctc acttcgcgta gaagcctggt aaatccctgg 7680 accctgagag acctaaaaaa aatattacaa gactatggta ttgagtatat catttttcct 7740 tcgaatgact tttttattta tgaagacgaa cgtcttttaa tgttttcaaa aaaatggacc 7800 aactttttta cgttacatga gttattggat gacctcgaaa ctattgagac aaaggcatcg 7860 tccacaacat cgacaaaaaa aaagcccaca attaccaagc aagagcttta ctccttagta 7920 gcggcagata cccagttaaa taaagcattg attgaaagaa tctttacaag tcagcaaaaa 7980 ataatacaaa atgctttaaa gcacaatcaa gaagttatta taccacccgg aatcaagttc 8040 accgtcgtta cggtgaaagc taaacctgct cgccagggcc ataatcccgc cacaggagag 8100 cctattcaaa ttaaagctaa acctgaacat aaagccgtaa agatacgagc attgaaacct 8160 gtccatgata tgttaaacgt tgagccacgc gaacagtttt ttcaagatct gctttcagca 8220 gtggatcaac aaatggacac tgtaaaaaat gacataaaag acattatgaa agaaaaaacg 8280 tcttttatgg tatcattcga aaactttata gaacgttacg ataccatgga aaaaaatatt 8340 caagaccttc agaataagta cgaagaaatg gcggccaacc ttatgaccgt catgacggat 8400 acaaaaattc agcttggagc cattatcgcc caacttgaga ttctaatgat aaatggcact 8460 ccacttccgg caaaaaagac aacaattaag gaggctatgc ccttaccttc atcaaacacg 8520 aataatgaac aaacgagtcc tcccgcctca ggcaaaacaa gtgaaacacc taaaaaaaat 8580 cccacgaatg cgatgttctt cacgcgtagc gaatgggcat cctcgaatac ttttcgagaa 8640 aagtttttaa caccagaaat tcaagccata ttggatgagc agtttgcaaa caagaccggg 8700 atcgaaagat tgcatgccga gggtctttac atgtggagaa cccaattctc tgacgaacag 8760 aagaaaatgg tcaaagagat gatgaagaag 8790 <210> 37 <211> 2919 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 2' <400> 37 cagatctttg taaagacctt gactggtaag accatcaccc tggaagtgga gcccagtgac 60 accattgaga atgtcaaggg gaagattcaa gaaaaagagg gcatcccccc agaccagcag 120 aggctgatct ttgccgggaa gcagctggaa gatgggcgca ccctgtctga ctacaacatc 180 cagaaggagt ccaccctgca cctggtcctc cgcctcagag gtgccgattt tattttaaat 240 atatccatga aaatggaggt catcttcaaa acggatttaa gatcatcttc acaagttgtg 300 tttcatgcgg gtagcctgta taattggttt tctgttgaga ttatcaatag cggtagaatt 360 gttacgaccg ctataaaaac attgcttagt actgttaagt atgatattgt gaaatctgct 420 cgtatatatg cagggcaagg gtatactgaa catcaggctc aagaagaatg gaatatgatt 480 ctgcatgtgc tgtttgaaga ggagacggaa tcctcagcat cttcggagtc aattcatgaa 540 aaaaatgata atgaaaccaa tgaatgcaca tcctcctttg aaacgttgtt tgagcaagag 600 ccctcatcgg agacacctaa agactccaag ctgtatatgc ttgcacaaaa gactgtgcaa 660 catattgaac aatatggaaa ggcacctgat tttaacaagg ttattagagc acataatttt 720 attcaaacca ttcatggaac ccctctaaag gaagaagaaa aagaggtggt aagactcatg 780 gttattaaac ttttaaaaaa aaaagattct gaattttttc aaccggttta tccgcggcat 840 tatggtgagt gtttgtcacc agtcactaca ccaagcttct tctccacaca tatgtatact 900 attctcattg ctatcgtggt cttagtcatc attatcatcg ttctaatcta tctattctct 960 tcaagaaaga aaaaagctgc tgctgctatt gaggaggaag atatacagtt tataaatcct 1020 tatcaagatc agcagtgggc agaagtcact ccacaaccag gtacctctaa accagctgga 1080 gcgactacag caagtgtagg caagccagtc acgggcagac cggcaacaaa cagaccagca 1140 acaaacaaac cagttacgga caacccagtt acggacagac tagtcatggc aactggcggg 1200 ccggcggccg cacctgcggc cgcgagtgct cctgctcatc cggctgagcc ttacacgaca 1260 gtcactactc agaacactgc ttcacaaaca atgtcggcta ttgaaaattt acgacaaaga 1320 aacacctata cgcataaaga cctagaaaac tccttgtttt ctaagaaaaa gtacatcggt 1380 cttatcgata agaagtgtga attgaaaaaa atactagatg attatagtat attaataatt 1440 ggaatattaa ttggaactaa catcataacc cttatttata attttatagg atggattaat 1500 tatataaaat gttaccaaaa tgatgataag atgttttatt gccctaaaga ttgggttgga 1560 tataataatg tttgttatta ttttagtaat gaagaaaaaa attatactaa tgcaagtaat 1620 tattgtaagc aattaaatag tacgcttgct aataataata ctaatttagt aaatcttact 1680 aaaacattaa atcttactaa aacatataat cacgaatcta attattgggt taattattct 1740 ttaattaaaa atgagtcagt actattacgt aatagtggat attacaaaaa acaaaaacat 1800 gtaagtttat tatatatttg tagtaaaata atacttattt ttttaatatt ttctaacata 1860 gttttaagta ttatatattg ggttagtttt aataaaacaa taattttaga tagtaatatt 1920 actaatgata ataatgatat aaatggagta tcatggaata cttattttaa taattctttt 1980 aatacactag ctacatgtgg aaaagcaggt aacttttgtg aatgttctaa ttatagtaca 2040 tcactatata atataacaaa taattgtagc ttaactattt ttcctcataa tactgatata 2100 tttaatacaa catatcaact agtatatcta aatcaaaaaa ttaattatac aataaaatta 2160 ttaacacctg tagattctcc aaatatcaca tataattgta ctaattcttt aataacatgt 2220 aaaaaaaata atggaacaaa cactaatata tatttaaata taaatgatac tatagttaat 2280 tatactaatg aaagtatact taattattat tggaataata gtaacattaa caattttaca 2340 gctacatgta taattaataa tacaattagt tcttctaatg aaacagaact tataaattgt 2400 actaatccca cattgaaata tcaaaactat ttttatactt tttttaaatt attttatatt 2460 ataatattta tcgtatctgg gataataata agtattcttt ttatatccat cataactttt 2520 ttatctttac gaaaaagaaa aaaacatgtt gaagaaatag aaagtccacc accttctgaa 2580 tctaatgaag aagaacaatg tcagcatgat gacaccactt ccatacatga accatctccc 2640 agagaaccat tacttcctaa gccttacagt cgttatcagt ataatacacc tatttactac 2700 atgcgtccct caacacaacc actcaaccca tttcccttac ctaaaccgtg tcctccaccc 2760 aaaccatgtc cgccacccaa accatgtcct ccacctaaac catgtccttc agctgaatcc 2820 tattctccac ccaaaccact acctagtatc ccgctactac ccaatatccc gccattatct 2880 acccaaaata tttcgcttat tcacgtagat agaattatt 2919 <210> 38 <211> 6096 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 3' <400> 38 cagatctttg taaagacctt gactggtaag accatcaccc tggaagtgga gcccagtgac 60 accattgaga atgtcaaggg gaagattcaa gaaaaagagg gcatcccccc agaccagcag 120 aggctgatct ttgccgggaa gcagctggaa gatgggcgca ccctgtctga ctacaacatc 180 cagaaggagt ccaccctgca cctggtcctc cgcctcagag gtgcctatca tgattatgct 240 tcaaagctgt tagccgatta taggtcagat ccacctcttt gggaaagcga tttgccgcgt 300 cataaccggt attctgataa tatactaaat tcccggtact gcggcaacaa aaatggagct 360 gcgcctgtat acaatgaata cactaatagt cctgaaaagg ctgaaaaggg attgcaactc 420 agtgatctac gtaacttttc ttttatgctg aaccctcagc acaaaaacat tggttacggc 480 gatgcgcaag atctggagcc gtacagctcc attccaaaaa ataaactttt taatcatttt 540 aaaaatcatc gtcctgcttt ttcaacccat acggaaaacc taattaggcg caatgttgtt 600 cgtacggaaa aaaaaacatt tccgcaggtg gctagcttaa agggcacaca gaaaaattgt 660 ctgacccagc cttcctcatt gccttcctta aaaaacccaa aaaatagttc ggttccttct 720 acgaggttca gcgagcacac aaaatttttt tcgtacgagg atcttcctaa actcagaaca 780 aagggtacca ttaagcatga gcagcacctt ggcgatcaga tgccgggcca gcactataac 840 ggatatattc cgcacaaaga cgtgtacaat attttgtgtt tagcgcacaa tctacctgcg 900 tcggtagaaa aagggattgc aggtagaggt attcctttag gaaatccaca tgtcaaaccg 960 aacatagagc aggaactcat aaaatccacc agtacctaca cggacgtgcc gatgcttggt 1020 ccccttccgc ccaaagattc gcaacacggc agagaatacc aggaattttc cgcaaataga 1080 catatgctac aagtatctaa tatactacat tctgtgtttg ctaatcattc tataaagcca 1140 caaatcctag aagacatacc ggtactcaat gcacaactaa caagcataaa gccggtaagc 1200 ccatttttaa acaaagcgta tcaaacccat tatatggaaa atattgttac cttggtgccc 1260 cggtttaaga gtattgccaa ctactcaagc cctataccga attattctaa aagaaatagc 1320 ggtcaggctg aatattttga tacctcaaag caaacaattt ctagacacaa taattatata 1380 cctaaatata cgggaggaat tggagattcc aaactagaca gcacctttcc taaagatttc 1440 aacgcctcct cggtaccgtt aacaagcgcg gaaaaggacc attccctgcg gggagacaac 1500 tcagcatgtt gcatctcatc tatatctcca tcattgccct ctaatatgaa acagttttgc 1560 aagatttctg tatggctaca gcagcacgat ccagatttat tagaaattat caacaactta 1620 tgtatgcttg gcaatttatc cgcggcaaag tacaaacacg gagttacctt catttacccc 1680 aaacaggcaa agatccgcga tgaaataaaa aaacatgcct actccaatga cccttcacaa 1740 gccataaaga ccttagaatc actcatcctt ccattttaca ttcccactcc agcggagttc 1800 accggggaaa tcggctccta caccggagtg aaattagagg ttgaaaaaac ggaggcgaat 1860 aaagttattt taaaaaatgg agaagcggtc ctagtaccgg cggccgattt taagcccttt 1920 cctgatcgcc gactagcggt ctggatcatg gagtcaggct ctatgcccct ggagggtccc 1980 ccctataagc ggaaaaagga gggtgggggg aatgacccgc cggttcctaa gcatatctcg 2040 ccgtatactc cgcgcacgcg tattgccatt gaggtggaaa aggcctttga tgactgtatg 2100 cgtcaaaact ggtgtagtgt caataatccc tatcttgcca agtcggtctc cttgctgtct 2160 ttcttgtcgc tcaaccatcc caccgagttt attaaggtac tgccgcttat agactttgac 2220 cccttggtga ccttttatct acttcttgag ccctataaaa cgcatgggga tgacttttta 2280 attccggaaa ccattttatt cggccctacc ggatggaatg gtacagatct gtatcaaagt 2340 gccatgctgg agtttaaaaa gttttttacc cagattactc gccaaacctt tatggacata 2400 gccgattcgg ctactaagga ggtagatgtt cccatatgtt actcggatcc cgaaaccgta 2460 cattcctatg ccaatcacgt gcgtactgaa attttgcatc acaatgccgt caataaggtt 2520 acaacaccta acctcgtcgt gcaggcctat aatgagctcg agcaaaccaa taccatacga 2580 cattacggcc ctattttccc ggaaagtacc atcaacgcac tgcgtttttg gaaaaagctg 2640 tggcaggatg aacagcgatt tgttatccac ggcctgcacc gcacgttgat ggatcaaccc 2700 acctatgaaa cctctgagtt tgcagagatc gttagaaatt tacggttttc gcgtcccggc 2760 aataactata taaacgagct taatattaca agtcccgcta tgtacggcga caagcatacc 2820 accggagata ttgcgcccaa tgatagattt gccatgttgg tggcctttat caacagtact 2880 gactttttat acaccgcgat tcccgaggaa aaggtagggg ggaatgaaac ccaaaccagt 2940 agccttacag acctagttcc aacacggcta cactcttttt taaatcataa tctaagcaaa 3000 cttaaaatct taaaccgcgc gcagcaaacg gttagaaata ttctttcaaa tgattgtctt 3060 aatcaactga aacattatgt taaacacacg ggaaaaaatg aaatactaaa gttacttcaa 3120 gaatattttc tagtagcaga tcatcgagaa catcatgtga ttccttttct taaaaccgat 3180 ttccatcaca tgcatcaaaa tcctatacaa aaaaaacaag ctctcctaga aatcaaacag 3240 ctttttactg gagattatct catctgcaaa agcccttcta ccattctggc ctgtattgaa 3300 cgaaaaacct acaaagactt tgcggcttct ttgaaagatg gacgttataa aaatcgccaa 3360 aaaatgctgt cgctgcgaga acaaaccaac tgtcaacttt atttttttgt agaaggcccg 3420 gcatttccta accctcaaaa aaaaattaat cacgttgcct atgcaagcat tattactgct 3480 atgacgcatc ttatggttag agatcatatt tttgtcattc aaacgaaaaa tgaggcccac 3540 agttcccaaa agcttgtgca gcttttttat gccttttcta aggaaatggt gtgcgtcgtt 3600 cccacctccc tcacccccac ggatgaagag ctatgcatca agctatggtc ttctctttct 3660 ggtatttcag gcgtgatagg taaaatcttg gcaaacactt gttccgtagc tcatttggtt 3720 tctggaaagc tttcatcgca gaatattgat cagttaaaaa ctccctccaa ccgaccattc 3780 cccaaaaaag taaaacgtat gcttataagc attagcaaag gaaataagga gttagaaata 3840 aaattgctct cgggggttcc caatatcggg aaaaaattag ctgccgaaat tttaaaagat 3900 catgcgcttc ttttttttct aaatcagccc gtagaatgct tggcaaatat acaaatcgtt 3960 caaaaaaccc gtacgattaa gttgggaatg aagcgagccg aagcgattca ttatttttta 4020 aactggtgtg gctctgccca tgtaaccgat gatagccaaa atatcacaga ggcgtcgcgg 4080 cctacaatgc aggtcgcgac gcagtccgcc gcaactcagc ccgctgcaac gcagccattg 4140 cacgaagtat cagatgatgc atcatcagat gcttcatcac ccgtagggca tcaaacatta 4200 tctaaagaaa tgttattgaa cacagccgtt gagacacaaa tggacaaact tggttttctg 4260 ctaaatcaca taggtaaaca ggttaccact aaggtgctta gcaatgccca tataactcaa 4320 acgatgaagg agattatttt ggaaaatcat agtgtagatg gtggagccgc aaaaaatgtt 4380 tcaaaaggga agtcttcccc aaaagaaaaa aaacattgga ctgagttcga atcctgggaa 4440 cagctcagca agtctaaaag aagttttaag gaatactggg cggagcgtaa tgagattgtg 4500 aatactctgt tgcttaactg ggacaatgtt cggggtgcca tcaaaaaatt tttggacgat 4560 gaccgtgaat ggtgcggccg cattaatatg ataaacggtg tacccgagat agtggaaatc 4620 attccaagcc cctatagggc aggagagaac atttattttg gcagcgaggc tatgatgcct 4680 gctgatattt atagcagggt ggccaacaag cctgctatgt ttgtgtttca cacgcatcct 4740 aatttgggtt catgttgtgg aggaatgcct tccatatgtg acatttctac aacgctgcgt 4800 tatctactca tggggtggac tgctgggcat ctaatcattt cttcgaatca agtaggaatg 4860 ctcacggttg ataagagaat tattgttgat ttgtgggcca atgagaatcc gcgctggctt 4920 atggcgcaaa aaatattaga tatttttatg atgctcactt cgcgtagaag cctggtaaat 4980 ccctggaccc tgagagacct aaaaaaaata ttacaagact atggtattga gtatatcatt 5040 tttccttcga atgacttttt tatttatgaa gacgaacgtc ttttaatgtt ttcaaaaaaa 5100 tggaccaact tttttacgtt acatgagtta ttggatgacc tcgaaactat tgagacaaag 5160 gcatcgtcca caacatcgac aaaaaaaaag cccacaatta ccaagcaaga gctttactcc 5220 ttagtagcgg cagataccca gttaaataaa gcattgattg aaagaatctt tacaagtcag 5280 caaaaaataa tacaaaatgc tttaaagcac aatcaagaag ttattatacc acccggaatc 5340 aagttcaccg tcgttacggt gaaagctaaa cctgctcgcc agggccataa tcccgccaca 5400 ggagagccta ttcaaattaa agctaaacct gaacataaag ccgtaaagat acgagcattg 5460 aaacctgtcc atgatatgtt aaacgttgag ccacgcgaac agttttttca agatctgctt 5520 tcagcagtgg atcaacaaat ggacactgta aaaaatgaca taaaagacat tatgaaagaa 5580 aaaacgtctt ttatggtatc attcgaaaac tttatagaac gttacgatac catggaaaaa 5640 aatattcaag accttcagaa taagtacgaa gaaatggcgg ccaaccttat gaccgtcatg 5700 acggatacaa aaattcagct tggagccatt atcgcccaac ttgagattct aatgataaat 5760 ggcactccac ttccggcaaa aaagacaaca attaaggagg ctatgccctt accttcatca 5820 aacacgaata atgaacaaac gagtcctccc gcctcaggca aaacaagtga aacacctaaa 5880 aaaaatccca cgaatgcgat gttcttcacg cgtagcgaat gggcatcctc gaatactttt 5940 cgagaaaagt ttttaacacc agaaattcaa gccatattgg atgagcagtt tgcaaacaag 6000 accgggatcg aaagattgca tgccgagggt ctttacatgt ggagaaccca attctctgac 6060 gaacagaaga aaatggtcaa agagatgatg aagaag 6096 <210> 39 <211> 4506 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 4' <400> 39 cagatctttg taaagacctt gactggtaag accatcaccc tggaagtgga gcccagtgac 60 accattgaga atgtcaaggg gaagattcaa gaaaaagagg gcatcccccc agaccagcag 120 aggctgatct ttgccgggaa gcagctggaa gatgggcgca ccctgtctga ctacaacatc 180 cagaaggagt ccaccctgca cctggtcctc cgcctcagag gtgccgattt tattttaaat 240 atatccatga aaatggaggt catcttcaaa acggatttaa gatcatcttc acaagttgtg 300 tttcatgcgg gtagcctgta taattggttt tctgttgaga ttatcaatag cggtagaatt 360 gttacgaccg ctataaaaac attgcttagt actgttaagt atgatattgt gaaatctgct 420 cgtatatatg cagggcaagg gtatactgaa catcaggctc aagaagaatg gaatatgatt 480 ctgcatgtgc tgtttgaaga ggagacggaa tcctcagcat cttcggagtc aattcatgaa 540 aaaaatgata atgaaaccaa tgaatgcaca tcctcctttg aaacgttgtt tgagcaagag 600 ccctcatcgg agacacctaa agactccaag ctgtatatgc ttgcacaaaa gactgtgcaa 660 catattgaac aatatggaaa ggcacctgat tttaacaagg ttattagagc acataatttt 720 attcaaacca ttcatggaac ccctctaaag gaagaagaaa aagaggtggt aagactcatg 780 gttattaaac ttttaaaaaa aaaagattct gaattttttc aaccggttta tccgcggcat 840 tatggtgagt gtttgtcacc agtcactaca ccaagcttct tctccacaca tatgtatact 900 attctcattg ctatcgtggt cttagtcatc attatcatcg ttctaatcta tctattctct 960 tcaagaaaga aaaaagctgc tgctgctatt gaggaggaag atatacagtt tataaatcct 1020 tatcaagatc agcagtgggc agaagtcact ccacaaccag gtacctctaa accagctgga 1080 gcgactacag caagtgtagg caagccagtc acgggcagac cggcaacaaa cagaccagca 1140 acaaacaaac cagttacgga caacccagtt acggacagac tagtcatggc aactggcggg 1200 ccggcggccg cacctgcggc cgcgagtgct cctgctcatc cggctgagcc ttacacgaca 1260 gtcactactc agaacactgc ttcacaaaca atgtcggcta ttgaaaattt acgacaaaga 1320 aacacctata cgcataaaga cctagaaaac tccttgccct ctaatatgaa acagttttgc 1380 aagatttctg tatggctaca gcagcacgat ccagatttat tagaaattat caacaactta 1440 tgtatgcttg gcaatttatc cgcggcaaag tacaaacacg gagttacctt catttacccc 1500 aaacaggcaa agatccgcga tgaaataaaa aaacatgcct actccaatga cccttcacaa 1560 gccataaaga ccttagaatc actcatcctt ccattttaca ttcccactcc agcggagttc 1620 accggggaaa tcggctccta caccggagtg aaattagagg ttgaaaaaac ggaggcgaat 1680 aaagttattt taaaaaatgg agaagcggtc ctagtaccgg cggccgattt taagcccttt 1740 cctgatcgcc gactagcggt ctggatcatg gagtcaggct ctatgcccct ggagggtccc 1800 ccctataagc ggaaaaagga gggtgggggg aatgacccgc cggttcctaa gcatatctcg 1860 ccgtatactc cgcgcacgcg tattgccatt gaggtggaaa aggcctttga tgactgtatg 1920 cgtcaaaact ggtgtagtgt caataatccc tatcttgcca agtcggtctc cttgctgtct 1980 ttcttgtcgc tcaaccatcc caccgagttt attaaggtac tgccgcttat agactttgac 2040 cccttggtga ccttttatct acttcttgag ccctataaaa cgcatgggga tgacttttta 2100 attccggaaa ccattttatt cggccctacc ggatggaatg gtacagatct gtatcaaagt 2160 gccatgctgg agtttaaaaa gttttttacc cagattactc gccaaacctt tatggacata 2220 gccgattcgg ctactaagga ggtagatgtt cccatatgtt actcggatcc cgaaaccgta 2280 cattcctatg ccaatcacgt gcgtactgaa attttgcatc acaatgccgt caataaggtt 2340 acaacaccta acctcgtcgt gcaggcctat aatgagctcg agcaaaccaa taccatacga 2400 cattacggcc ctattttccc ggaaagtacc atcaacgcac tgcgtttttg gaaaaagctg 2460 tggcaggatg aacagcgatt tgttatccac ggcctgcacc gcacgttgat ggatcaaccc 2520 acctatgaaa cctctgagtt tgcagagatc gttagaaatt tacggttttc gcgtcccggc 2580 aataactata taaacgagct taatattaca agtcccgcta tgtacggcga caagcatacc 2640 accggagata ttgcgcccaa tgatagattt gccatgttgg tggcctttat caacagtact 2700 gactttttat acaccgcgat tcccgaggaa aaggtagggg ggaatgaaac ccaaaccagt 2760 agccttacag acctagttcc aacacggcta cactcttttt taaatcataa tctaagcaaa 2820 cttaaaatct taaaccgcgc gcagcaaacg gttagaaata ttctttcaaa tgattgtctt 2880 aatcaactga aacattatgt taaacacacg ggaaaaaatg aaatactaaa gttacttcaa 2940 gaattttcta agaaaaagta catcggtctt atcgataaga agtgtgaatt gaaaaaaata 3000 ctagatgatt atagtatatt aataattgga atattaattg gaactaacat cataaccctt 3060 atttataatt ttataggatg gattaattat ataaaatgtt accaaaatga tgataagatg 3120 ttttattgcc ctaaagattg ggttggatat aataatgttt gttattattt tagtaatgaa 3180 gaaaaaaatt atactaatgc aagtaattat tgtaagcaat taaatagtac gcttgctaat 3240 aataatacta atttagtaaa tcttactaaa acattaaatc ttactaaaac atataatcac 3300 gaatctaatt attgggttaa ttattcttta attaaaaatg agtcagtact attacgtaat 3360 agtggatatt acaaaaaaca aaaacatgta agtttattat atatttgtag taaaataata 3420 cttatttttt taatattttc taacatagtt ttaagtatta tatattgggt tagttttaat 3480 aaaacaataa ttttagatag taatattact aatgataata atgatataaa tggagtatca 3540 tggaatactt attttaataa ttcttttaat acactagcta catgtggaaa agcaggtaac 3600 ttttgtgaat gttctaatta tagtacatca ctatataata taacaaataa ttgtagctta 3660 actatttttc ctcataatac tgatatattt aatacaacat atcaactagt atatctaaat 3720 caaaaaatta attatacaat aaaattatta acacctgtag attctccaaa tatcacatat 3780 aattgtacta attctttaat aacatgtaaa aaaaataatg gaacaaacac taatatatat 3840 ttaaatataa atgatactat agttaattat actaatgaaa gtatacttaa ttattattgg 3900 aataatagta acattaacaa ttttacagct acatgtataa ttaataatac aattagttct 3960 tctaatgaaa cagaacttat aaattgtact aatcccacat tgaaatatca aaactatttt 4020 tatacttttt ttaaattatt ttatattata atatttatcg tatctgggat aataataagt 4080 attcttttta tatccatcat aactttttta tctttacgaa aaagaaaaaa acatgttgaa 4140 gaaatagaaa gtccaccacc ttctgaatct aatgaagaag aacaatgtca gcatgatgac 4200 accacttcca tacatgaacc atctcccaga gaaccattac ttcctaagcc ttacagtcgt 4260 tatcagtata atacacctat ttactacatg cgtccctcaa cacaaccact caacccattt 4320 cccttaccta aaccgtgtcc tccacccaaa ccatgtccgc cacccaaacc atgtcctcca 4380 cctaaaccat gtccttcagc tgaatcctat tctccaccca aaccactacc tagtatcccg 4440 ctactaccca atatcccgcc attatctacc caaaatattt cgcttattca cgtagataga 4500 attatt 4506 <210> 40 <211> 4509 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 5' <400> 40 cagatctttg taaagacctt gactggtaag accatcaccc tggaagtgga gcccagtgac 60 accattgaga atgtcaaggg gaagattcaa gaaaaagagg gcatcccccc agaccagcag 120 aggctgatct ttgccgggaa gcagctggaa gatgggcgca ccctgtctga ctacaacatc 180 cagaaggagt ccaccctgca cctggtcctc cgcctcagag gtgcctatca tgattatgct 240 tcaaagctgt tagccgatta taggtcagat ccacctcttt gggaaagcga tttgccgcgt 300 cataaccggt attctgataa tatactaaat tcccggtact gcggcaacaa aaatggagct 360 gcgcctgtat acaatgaata cactaatagt cctgaaaagg ctgaaaaggg attgcaactc 420 agtgatctac gtaacttttc ttttatgctg aaccctcagc acaaaaacat tggttacggc 480 gatgcgcaag atctggagcc gtacagctcc attccaaaaa ataaactttt taatcatttt 540 aaaaatcatc gtcctgcttt ttcaacccat acggaaaacc taattaggcg caatgttgtt 600 cgtacggaaa aaaaaacatt tccgcaggtg gctagcttaa agggcacaca gaaaaattgt 660 ctgacccagc cttcctcatt gccttcctta aaaaacccaa aaaatagttc ggttccttct 720 acgaggttca gcgagcacac aaaatttttt tcgtacgagg atcttcctaa actcagaaca 780 aagggtacca ttaagcatga gcagcacctt ggcgatcaga tgccgggcca gcactataac 840 ggatatattc cgcacaaaga cgtgtacaat attttgtgtt tagcgcacaa tctacctgcg 900 tcggtagaaa aagggattgc aggtagaggt attcctttag gaaatccaca tgtcaaaccg 960 aacatagagc aggaactcat aaaatccacc agtacctaca cggacgtgcc gatgcttggt 1020 ccccttccgc ccaaagattc gcaacacggc agagaatacc aggaattttc cgcaaataga 1080 catatgctac aagtatctaa tatactacat tctgtgtttg ctaatcattc tataaagcca 1140 caaatcctag aagacatacc ggtactcaat gcacaactaa caagcataaa gccggtaagc 1200 ccatttttaa acaaagcgta tcaaacccat tatatggaaa atattgttac cttggtgccc 1260 cggtttaaga gtattgccaa ctactcaagc cctataccga attattctaa aagaaatagc 1320 ggtcaggctg aatattttga tacctcaaag caaacaattt ctagacacaa taattatata 1380 cctaaatata cgggaggaat tggagattcc aaactagaca gcacctttcc taaagatttc 1440 aacgcctcct cggtaccgtt aacaagcgcg gaaaaggacc attccctgcg gggagacaac 1500 tcagcatgtt gcatctcatc tatatctcca tcattgtatt ttctagtagc agatcatcga 1560 gaacatcatg tgattccttt tcttaaaacc gatttccatc acatgcatca aaatcctata 1620 caaaaaaaac aagctctcct agaaatcaaa cagcttttta ctggagatta tctcatctgc 1680 aaaagccctt ctaccattct ggcctgtatt gaacgaaaaa cctacaaaga ctttgcggct 1740 tctttgaaag atggacgtta taaaaatcgc caaaaaatgc tgtcgctgcg agaacaaacc 1800 aactgtcaac tttatttttt tgtagaaggc ccggcatttc ctaaccctca aaaaaaaatt 1860 aatcacgttg cctatgcaag cattattact gctatgacgc atcttatggt tagagatcat 1920 atttttgtca ttcaaacgaa aaatgaggcc cacagttccc aaaagcttgt gcagcttttt 1980 tatgcctttt ctaaggaaat ggtgtgcgtc gttcccacct ccctcacccc cacggatgaa 2040 gagctatgca tcaagctatg gtcttctctt tctggtattt caggcgtgat aggtaaaatc 2100 ttggcaaaca cttgttccgt agctcatttg gtttctggaa agctttcatc gcagaatatt 2160 gatcagttaa aaactccctc caaccgacca ttccccaaaa aagtaaaacg tatgcttata 2220 agcattagca aaggaaataa ggagttagaa ataaaattgc tctcgggggt tcccaatatc 2280 gggaaaaaat tagctgccga aattttaaaa gatcatgcgc ttcttttttt tctaaatcag 2340 cccgtagaat gcttggcaaa tatacaaatc gttcaaaaaa cccgtacgat taagttggga 2400 atgaagcgag ccgaagcgat tcattatttt ttaaactggt gtggctctgc ccatgtaacc 2460 gatgatagcc aaaatatcac agaggcgtcg cggcctacaa tgcaggtcgc gacgcagtcc 2520 gccgcaactc agcccgctgc aacgcagcca ttgcacgaag tatcagatga tgcatcatca 2580 gatgcttcat cacccgtagg gcatcaaaca ttatctaaag aaatgttatt gaacacagcc 2640 gttgagacac aaatggacaa acttggtttt ctgctaaatc acataggtaa acaggttacc 2700 actaaggtgc ttagcaatgc ccatataact caaacgatga aggagattat tttggaaaat 2760 catagtgtag atggtggagc cgcaaaaaat gtttcaaaag ggaagtcttc cccaaaagaa 2820 aaaaaacatt ggactgagtt cgaatcctgg gaacagctca gcaagtctaa aagaagtttt 2880 aaggaatact gggcggagcg taatgagatt gtgaatactc tgttgcttaa ctgggacaat 2940 gttcggggtg ccatcaaaaa atttttggac gatgaccgtg aatggtgcgg ccgcattaat 3000 atgataaacg gtgtacccga gatagtggaa atcattccaa gcccctatag ggcaggagag 3060 aacatttatt ttggcagcga ggctatgatg cctgctgata tttatagcag ggtggccaac 3120 aagcctgcta tgtttgtgtt tcacacgcat cctaatttgg gttcatgttg tggaggaatg 3180 ccttccatat gtgacatttc tacaacgctg cgttatctac tcatggggtg gactgctggg 3240 catctaatca tttcttcgaa tcaagtagga atgctcacgg ttgataagag aattattgtt 3300 gatttgtggg ccaatgagaa tccgcgctgg cttatggcgc aaaaaatatt agatattttt 3360 atgatgctca cttcgcgtag aagcctggta aatccctgga ccctgagaga cctaaaaaaa 3420 atattacaag actatggtat tgagtatatc atttttcctt cgaatgactt ttttatttat 3480 gaagacgaac gtcttttaat gttttcaaaa aaatggacca acttttttac gttacatgag 3540 ttattggatg acctcgaaac tattgagaca aaggcatcgt ccacaacatc gacaaaaaaa 3600 aagcccacaa ttaccaagca agagctttac tccttagtag cggcagatac ccagttaaat 3660 aaagcattga ttgaaagaat ctttacaagt cagcaaaaaa taatacaaaa tgctttaaag 3720 cacaatcaag aagttattat accacccgga atcaagttca ccgtcgttac ggtgaaagct 3780 aaacctgctc gccagggcca taatcccgcc acaggagagc ctattcaaat taaagctaaa 3840 cctgaacata aagccgtaaa gatacgagca ttgaaacctg tccatgatat gttaaacgtt 3900 gagccacgcg aacagttttt tcaagatctg ctttcagcag tggatcaaca aatggacact 3960 gtaaaaaatg acataaaaga cattatgaaa gaaaaaacgt cttttatggt atcattcgaa 4020 aactttatag aacgttacga taccatggaa aaaaatattc aagaccttca gaataagtac 4080 gaagaaatgg cggccaacct tatgaccgtc atgacggata caaaaattca gcttggagcc 4140 attatcgccc aacttgagat tctaatgata aatggcactc cacttccggc aaaaaagaca 4200 acaattaagg aggctatgcc cttaccttca tcaaacacga ataatgaaca aacgagtcct 4260 cccgcctcag gcaaaacaag tgaaacacct aaaaaaaatc ccacgaatgc gatgttcttc 4320 acgcgtagcg aatgggcatc ctcgaatact tttcgagaaa agtttttaac accagaaatt 4380 caagccatat tggatgagca gtttgcaaac aagaccggga tcgaaagatt gcatgccgag 4440 ggtctttaca tgtggagaac ccaattctct gacgaacaga agaaaatggt caaagagatg 4500 atgaagaag 4509 <210> 41 <211> 75 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF ubiquitin <400> 41 Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 1 5 10 15 Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu Lys 20 25 30 Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 35 40 45 Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 50 55 60 Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala 65 70 75 <210> 42 <211> 225 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF ubiquitin <400> 42 cagatctttg taaagacctt gactggtaag accatcaccc tggaagtgga gcccagtgac 60 accattgaga atgtcaaggg gaagattcaa gaaaaagagg gcatcccccc agaccagcag 120 aggctgatct ttgccgggaa gcagctggaa gatgggcgca ccctgtctga ctacaacatc 180 cagaaggagt ccaccctgca cctggtcctc cgcctcagag gtgcc 225 <210> 43 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Furin cleavage site <400> 43 Arg Gly Arg Lys Arg Arg Ser 1 5 <210> 44 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Furin cleavage site <400> 44 agaggaagaa agagaagatc a 21 <210> 45 <211> 2994 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 1'' <400> 45 Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 1 5 10 15 Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu 20 25 30 Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 35 40 45 Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 50 55 60 Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Asp Phe Ile Leu 65 70 75 80 Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser 85 90 95 Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser 100 105 110 Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr 115 120 125 Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr 130 135 140 Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met 145 150 155 160 Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser 165 170 175 Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser 180 185 190 Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Thr Pro Lys 195 200 205 Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu 210 215 220 Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn 225 230 235 240 Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu 245 250 255 Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys Lys Arg Gly Arg 260 265 270 Lys Arg Arg Ser Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His 275 280 285 Tyr Gly Glu Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr 290 295 300 His Met Tyr Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile 305 310 315 320 Ile Val Leu Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala 325 330 335 Ala Ile Glu Glu Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln 340 345 350 Gln Trp Ala Glu Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly 355 360 365 Ala Thr Thr Ala Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr 370 375 380 Asn Arg Pro Ala Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp 385 390 395 400 Arg Leu Val Met Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala 405 410 415 Ser Ala Pro Ala His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln 420 425 430 Asn Thr Ala Ser Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg 435 440 445 Asn Thr Tyr Thr His Lys Asp Leu Glu Asn Ser Leu Arg Gly Arg Lys 450 455 460 Arg Arg Ser Phe Ser Lys Lys Lys Tyr Ile Gly Leu Ile Asp Lys Lys 465 470 475 480 Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr Ser Ile Leu Ile Ile Gly 485 490 495 Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu Ile Tyr Asn Phe Ile Gly 500 505 510 Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn Asp Asp Lys Met Phe Tyr 515 520 525 Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr Phe Ser 530 535 540 Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser Asn Tyr Cys Lys Gln Leu 545 550 555 560 Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn Leu Val Asn Leu Thr Lys 565 570 575 Thr Leu Asn Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr Trp Val 580 585 590 Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asn Ser Gly 595 600 605 Tyr Tyr Lys Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys Ser Lys 610 615 620 Arg Gly Arg Lys Arg Arg Ser Ile Ile Leu Ile Phe Leu Ile Phe Ser 625 630 635 640 Asn Ile Val Leu Ser Ile Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile 645 650 655 Ile Leu Asp Ser Asn Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly Val 660 665 670 Ser Trp Asn Thr Tyr Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys 675 680 685 Gly Lys Ala Gly Asn Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu 690 695 700 Tyr Asn Ile Thr Asn Asn Cys Ser Leu Thr Ile Phe Pro His Asn Thr 705 710 715 720 Asp Ile Phe Asn Thr Thr Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile 725 730 735 Asn Tyr Thr Ile Lys Leu Leu Thr Pro Val Asp Ser Pro Asn Ile Thr 740 745 750 Tyr Asn Cys Thr Asn Ser Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr 755 760 765 Asn Thr Asn Ile Tyr Leu Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr 770 775 780 Asn Glu Ser Ile Leu Asn Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn 785 790 795 800 Phe Thr Ala Thr Cys Ile Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu 805 810 815 Thr Glu Leu Ile Asn Cys Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr 820 825 830 Phe Tyr Thr Phe Phe Lys Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser 835 840 845 Gly Ile Ile Ile Ser Ile Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser 850 855 860 Leu Arg Lys Arg Lys Lys His Val Glu Glu Ile Glu Ser Pro Pro Pro 865 870 875 880 Ser Glu Ser Asn Glu Glu Glu Gln Cys Gln His Asp Asp Thr Thr Ser 885 890 895 Ile His Glu Pro Ser Pro Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser 900 905 910 Arg Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln 915 920 925 Pro Leu Asn Pro Phe Pro Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro 930 935 940 Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala 945 950 955 960 Glu Ser Tyr Ser Pro Pro Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro 965 970 975 Asn Ile Pro Pro Leu Ser Thr Gln Asn Ile Ser Leu Ile His Val Asp 980 985 990 Arg Ile Ile Arg Gly Arg Lys Arg Arg Ser Tyr His Asp Tyr Ala Ser 995 1000 1005 Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro Pro Leu Trp Glu Ser Asp 1010 1015 1020 Leu Pro Arg His Asn Arg Tyr Ser Asp Asn Ile Leu Asn Ser Arg Tyr 1025 1030 1035 1040 Cys Gly Asn Lys Asn Gly Ala Ala Pro Val Tyr Asn Glu Tyr Thr Asn 1045 1050 1055 Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln Leu Ser Asp Leu Arg Asn 1060 1065 1070 Phe Ser Phe Met Leu Asn Pro Gln His Lys Asn Ile Gly Tyr Gly Asp 1075 1080 1085 Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile Pro Lys Asn Lys Leu Phe 1090 1095 1100 Asn His Phe Lys Asn His Arg Pro Ala Phe Ser Thr His Thr Glu Asn 1105 1110 1115 1120 Leu Ile Arg Arg Asn Val Val Arg Thr Glu Lys Lys Thr Phe Pro Gln 1125 1130 1135 Val Ala Ser Leu Lys Gly Thr Gln Lys Asn Cys Leu Thr Gln Pro Ser 1140 1145 1150 Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn Ser Ser Val Pro Ser Thr 1155 1160 1165 Arg Phe Ser Glu His Thr Lys Phe Phe Ser Tyr Glu Asp Leu Pro Lys 1170 1175 1180 Leu Arg Thr Lys Gly Thr Ile Lys His Glu Gln His Leu Gly Asp Gln 1185 1190 1195 1200 Met Pro Gly Gln His Tyr Asn Gly Tyr Ile Pro His Lys Asp Val Tyr 1205 1210 1215 Asn Ile Leu Cys Leu Ala His Asn Leu Pro Ala Ser Val Glu Lys Gly 1220 1225 1230 Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn Pro His Val Lys Pro Asn 1235 1240 1245 Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser Thr Tyr Thr Asp Val Pro 1250 1255 1260 Met Leu Gly Pro Leu Pro Pro Lys Asp Ser Gln His Gly Arg Glu Tyr 1265 1270 1275 1280 Gln Glu Phe Ser Ala Asn Arg His Met Leu Gln Val Ser Asn Ile Leu 1285 1290 1295 His Ser Val Phe Ala Asn His Ser Ile Lys Pro Gln Ile Leu Glu Asp 1300 1305 1310 Ile Pro Val Leu Asn Ala Gln Leu Thr Ser Ile Lys Pro Val Ser Pro 1315 1320 1325 Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr Met Glu Asn Ile Val Thr 1330 1335 1340 Leu Val Pro Arg Phe Lys Ser Ile Ala Asn Tyr Ser Ser Pro Ile Pro 1345 1350 1355 1360 Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala Glu Tyr Phe Asp Thr Ser 1365 1370 1375 Lys Gln Thr Ile Ser Arg His Asn Asn Tyr Ile Pro Lys Tyr Thr Gly 1380 1385 1390 Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr Phe Pro Lys Asp Phe Asn 1395 1400 1405 Ala Ser Ser Val Pro Leu Thr Ser Ala Glu Lys Asp His Ser Leu Arg 1410 1415 1420 Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser Ile Ser Pro Ser Leu Arg 1425 1430 1435 1440 Gly Arg Lys Arg Arg Ser Pro Ser Asn Met Lys Gln Phe Cys Lys Ile 1445 1450 1455 Ser Val Trp Leu Gln Gln His Asp Pro Asp Leu Leu Glu Ile Ile Asn 1460 1465 1470 Asn Leu Cys Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys His Gly 1475 1480 1485 Val Thr Phe Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys 1490 1495 1500 Lys His Ala Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu 1505 1510 1515 1520 Ser Leu Ile Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly 1525 1530 1535 Glu Ile Gly Ser Tyr Thr Gly Val Lys Leu Glu Val Glu Lys Thr Glu 1540 1545 1550 Ala Asn Lys Val Ile Leu Lys Asn Gly Glu Ala Val Leu Val Pro Ala 1555 1560 1565 Ala Asp Phe Lys Pro Phe Pro Asp Arg Arg Leu Ala Val Trp Ile Met 1570 1575 1580 Glu Ser Gly Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys 1585 1590 1595 1600 Glu Gly Gly Gly Asn Asp Pro Pro Val Pro Lys His Ile Ser Pro Tyr 1605 1610 1615 Thr Pro Arg Thr Arg Ile Ala Ile Glu Val Glu Lys Ala Phe Asp Asp 1620 1625 1630 Cys Met Arg Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys 1635 1640 1645 Ser Val Ser Leu Leu Ser Phe Leu Ser Leu Asn His Pro Thr Glu Phe 1650 1655 1660 Ile Lys Val Leu Pro Leu Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr 1665 1670 1675 1680 Leu Leu Leu Glu Pro Tyr Lys Thr His Gly Asp Asp Phe Leu Ile Pro 1685 1690 1695 Glu Thr Ile Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr 1700 1705 1710 Gln Ser Ala Met Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg 1715 1720 1725 Gln Thr Phe Met Asp Ile Ala Asp Ser Ala Thr Lys Glu Val Asp Val 1730 1735 1740 Pro Ile Cys Tyr Ser Asp Pro Glu Thr Val His Ser Tyr Ala Asn His 1745 1750 1755 1760 Val Arg Thr Glu Ile Leu His His Asn Ala Val Asn Lys Val Thr Thr 1765 1770 1775 Pro Asn Leu Val Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr 1780 1785 1790 Ile Arg His Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu 1795 1800 1805 Arg Phe Trp Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe Val Ile His 1810 1815 1820 Gly Leu His Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu 1825 1830 1835 1840 Phe Ala Glu Ile Val Arg Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn 1845 1850 1855 Tyr Ile Asn Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys 1860 1865 1870 His Thr Thr Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala Met Leu Val 1875 1880 1885 Ala Phe Ile Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu 1890 1895 1900 Lys Val Gly Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp Leu Val 1905 1910 1915 1920 Pro Thr Arg Leu His Ser Phe Leu Asn His Asn Leu Ser Lys Leu Lys 1925 1930 1935 Ile Leu Asn Arg Ala Gln Gln Thr Val Arg Asn Ile Leu Ser Asn Asp 1940 1945 1950 Cys Leu Asn Gln Leu Lys His Tyr Val Lys His Thr Gly Lys Asn Glu 1955 1960 1965 Ile Leu Lys Leu Leu Gln Glu Arg Gly Arg Lys Arg Arg Ser Tyr Phe 1970 1975 1980 Leu Val Ala Asp His Arg Glu His His Val Ile Pro Phe Leu Lys Thr 1985 1990 1995 2000 Asp Phe His His Met His Gln Asn Pro Ile Gln Lys Lys Gln Ala Leu 2005 2010 2015 Leu Glu Ile Lys Gln Leu Phe Thr Gly Asp Tyr Leu Ile Cys Lys Ser 2020 2025 2030 Pro Ser Thr Ile Leu Ala Cys Ile Glu Arg Lys Thr Tyr Lys Asp Phe 2035 2040 2045 Ala Ala Ser Leu Lys Asp Gly Arg Tyr Lys Asn Arg Gln Lys Met Leu 2050 2055 2060 Ser Leu Arg Glu Gln Thr Asn Cys Gln Leu Tyr Phe Phe Val Glu Gly 2065 2070 2075 2080 Pro Ala Phe Pro Asn Pro Gln Lys Lys Ile Asn His Val Ala Tyr Ala 2085 2090 2095 Ser Ile Ile Thr Ala Met Thr His Leu Met Val Arg Asp His Ile Phe 2100 2105 2110 Val Ile Gln Thr Lys Asn Glu Ala His Ser Ser Gln Lys Leu Val Gln 2115 2120 2125 Leu Phe Tyr Ala Phe Ser Lys Glu Met Val Cys Val Val Pro Thr Ser 2130 2135 2140 Leu Thr Pro Thr Asp Glu Glu Leu Cys Ile Lys Leu Trp Ser Ser Leu 2145 2150 2155 2160 Ser Gly Ile Ser Gly Val Ile Gly Lys Ile Leu Ala Asn Thr Cys Ser 2165 2170 2175 Val Ala His Leu Val Ser Gly Lys Leu Ser Ser Gln Asn Ile Asp Gln 2180 2185 2190 Leu Lys Thr Pro Ser Asn Arg Pro Phe Pro Lys Lys Val Lys Arg Met 2195 2200 2205 Leu Ile Ser Ile Ser Lys Gly Asn Lys Glu Leu Glu Ile Lys Leu Leu 2210 2215 2220 Ser Gly Val Pro Asn Ile Gly Lys Lys Leu Ala Ala Glu Ile Leu Lys 2225 2230 2235 2240 Asp His Ala Leu Leu Phe Phe Leu Asn Gln Pro Val Glu Cys Leu Ala 2245 2250 2255 Asn Ile Gln Ile Val Gln Lys Thr Arg Thr Ile Lys Leu Gly Met Lys 2260 2265 2270 Arg Ala Glu Ala Ile His Tyr Phe Leu Asn Trp Cys Gly Ser Ala His 2275 2280 2285 Val Thr Asp Asp Ser Gln Asn Ile Thr Glu Ala Ser Arg Pro Thr Met 2290 2295 2300 Gln Val Ala Thr Gln Ser Ala Ala Thr Gln Pro Ala Ala Thr Gln Pro 2305 2310 2315 2320 Leu His Glu Val Ser Asp Asp Ala Ser Ser Asp Ala Ser Ser Pro Val 2325 2330 2335 Gly His Gln Thr Leu Ser Lys Glu Met Leu Leu Asn Thr Ala Arg Gly 2340 2345 2350 Arg Lys Arg Arg Ser Val Glu Thr Gln Met Asp Lys Leu Gly Phe Leu 2355 2360 2365 Leu Asn His Ile Gly Lys Gln Val Thr Thr Lys Val Leu Ser Asn Ala 2370 2375 2380 His Ile Thr Gln Thr Met Lys Glu Ile Ile Leu Glu Asn His Ser Val 2385 2390 2395 2400 Asp Gly Gly Ala Ala Lys Asn Val Ser Lys Gly Lys Ser Ser Pro Lys 2405 2410 2415 Glu Lys Lys His Trp Thr Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys 2420 2425 2430 Ser Lys Arg Ser Phe Lys Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val 2435 2440 2445 Asn Thr Leu Leu Leu Asn Trp Asp Asn Val Arg Gly Ala Ile Lys Lys 2450 2455 2460 Phe Leu Asp Asp Asp Arg Glu Trp Cys Gly Arg Ile Asn Met Ile Asn 2465 2470 2475 2480 Gly Val Pro Glu Ile Val Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly 2485 2490 2495 Glu Asn Ile Tyr Phe Gly Ser Glu Ala Met Met Pro Ala Asp Ile Tyr 2500 2505 2510 Ser Arg Val Ala Asn Lys Pro Ala Met Phe Val Phe His Thr His Pro 2515 2520 2525 Asn Leu Gly Ser Cys Cys Gly Gly Met Pro Ser Ile Cys Asp Ile Ser 2530 2535 2540 Thr Thr Leu Arg Tyr Leu Leu Met Gly Trp Thr Ala Gly His Leu Ile 2545 2550 2555 2560 Ile Ser Ser Asn Gln Val Gly Met Leu Thr Val Asp Lys Arg Ile Ile 2565 2570 2575 Val Asp Leu Trp Ala Asn Glu Asn Pro Arg Trp Leu Met Ala Gln Lys 2580 2585 2590 Ile Leu Asp Ile Phe Met Met Leu Thr Ser Arg Arg Ser Leu Val Asn 2595 2600 2605 Pro Trp Thr Leu Arg Asp Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile 2610 2615 2620 Glu Tyr Ile Ile Phe Pro Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu 2625 2630 2635 2640 Arg Leu Leu Met Phe Ser Lys Lys Trp Thr Asn Phe Phe Thr Leu His 2645 2650 2655 Glu Leu Leu Asp Asp Leu Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr 2660 2665 2670 Thr Arg Gly Arg Lys Arg Arg Ser Ser Thr Lys Lys Lys Pro Thr Ile 2675 2680 2685 Thr Lys Gln Glu Leu Tyr Ser Leu Val Ala Ala Asp Thr Gln Leu Asn 2690 2695 2700 Lys Ala Leu Ile Glu Arg Ile Phe Thr Ser Gln Gln Lys Ile Ile Gln 2705 2710 2715 2720 Asn Ala Leu Lys His Asn Gln Glu Val Ile Ile Pro Pro Gly Ile Lys 2725 2730 2735 Phe Thr Val Val Thr Val Lys Ala Lys Pro Ala Arg Gln Gly His Asn 2740 2745 2750 Pro Ala Thr Gly Glu Pro Ile Gln Ile Lys Ala Lys Pro Glu His Lys 2755 2760 2765 Ala Val Lys Ile Arg Ala Leu Lys Pro Val His Asp Met Leu Asn Arg 2770 2775 2780 Gly Arg Lys Arg Arg Ser Val Glu Pro Arg Glu Gln Phe Phe Gln Asp 2785 2790 2795 2800 Leu Leu Ser Ala Val Asp Gln Gln Met Asp Thr Val Lys Asn Asp Ile 2805 2810 2815 Lys Asp Ile Met Lys Glu Lys Thr Ser Phe Met Val Ser Phe Glu Asn 2820 2825 2830 Phe Ile Glu Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln Asp Leu Gln 2835 2840 2845 Asn Lys Tyr Glu Glu Met Ala Ala Asn Leu Met Thr Val Met Thr Asp 2850 2855 2860 Thr Lys Ile Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu Ile Leu Met 2865 2870 2875 2880 Ile Asn Gly Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile Lys Glu Ala 2885 2890 2895 Met Pro Leu Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr Ser Pro Pro 2900 2905 2910 Ala Ser Gly Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro Thr Asn Ala 2915 2920 2925 Met Phe Phe Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr Phe Arg Glu 2930 2935 2940 Lys Phe Leu Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu Gln Phe Ala 2945 2950 2955 2960 Asn Lys Thr Gly Ile Glu Arg Leu His Ala Glu Gly Leu Tyr Met Trp 2965 2970 2975 Arg Thr Gln Phe Ser Asp Glu Gln Lys Lys Met Val Lys Glu Met Met 2980 2985 2990 Lys Lys <210> 46 <211> 995 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 2'' <400> 46 Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 1 5 10 15 Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu 20 25 30 Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 35 40 45 Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 50 55 60 Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Asp Phe Ile Leu 65 70 75 80 Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser 85 90 95 Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser 100 105 110 Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr 115 120 125 Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr 130 135 140 Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met 145 150 155 160 Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser 165 170 175 Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser 180 185 190 Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Thr Pro Lys 195 200 205 Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu 210 215 220 Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn 225 230 235 240 Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu 245 250 255 Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys Lys Arg Gly Arg 260 265 270 Lys Arg Arg Ser Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His 275 280 285 Tyr Gly Glu Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr 290 295 300 His Met Tyr Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile 305 310 315 320 Ile Val Leu Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala 325 330 335 Ala Ile Glu Glu Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln 340 345 350 Gln Trp Ala Glu Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly 355 360 365 Ala Thr Thr Ala Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr 370 375 380 Asn Arg Pro Ala Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp 385 390 395 400 Arg Leu Val Met Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala 405 410 415 Ser Ala Pro Ala His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln 420 425 430 Asn Thr Ala Ser Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg 435 440 445 Asn Thr Tyr Thr His Lys Asp Leu Glu Asn Ser Leu Arg Gly Arg Lys 450 455 460 Arg Arg Ser Phe Ser Lys Lys Lys Tyr Ile Gly Leu Ile Asp Lys Lys 465 470 475 480 Cys Glu Leu Lys Lys Ile Leu Asp Asp Tyr Ser Ile Leu Ile Ile Gly 485 490 495 Ile Leu Ile Gly Thr Asn Ile Ile Thr Leu Ile Tyr Asn Phe Ile Gly 500 505 510 Trp Ile Asn Tyr Ile Lys Cys Tyr Gln Asn Asp Asp Lys Met Phe Tyr 515 520 525 Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr Phe Ser 530 535 540 Asn Glu Glu Lys Asn Tyr Thr Asn Ala Ser Asn Tyr Cys Lys Gln Leu 545 550 555 560 Asn Ser Thr Leu Ala Asn Asn Asn Thr Asn Leu Val Asn Leu Thr Lys 565 570 575 Thr Leu Asn Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr Trp Val 580 585 590 Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asn Ser Gly 595 600 605 Tyr Tyr Lys Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys Ser Lys 610 615 620 Arg Gly Arg Lys Arg Arg Ser Ile Ile Leu Ile Phe Leu Ile Phe Ser 625 630 635 640 Asn Ile Val Leu Ser Ile Ile Tyr Trp Val Ser Phe Asn Lys Thr Ile 645 650 655 Ile Leu Asp Ser Asn Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly Val 660 665 670 Ser Trp Asn Thr Tyr Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys 675 680 685 Gly Lys Ala Gly Asn Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Leu 690 695 700 Tyr Asn Ile Thr Asn Asn Cys Ser Leu Thr Ile Phe Pro His Asn Thr 705 710 715 720 Asp Ile Phe Asn Thr Thr Tyr Gln Leu Val Tyr Leu Asn Gln Lys Ile 725 730 735 Asn Tyr Thr Ile Lys Leu Leu Thr Pro Val Asp Ser Pro Asn Ile Thr 740 745 750 Tyr Asn Cys Thr Asn Ser Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr 755 760 765 Asn Thr Asn Ile Tyr Leu Asn Ile Asn Asp Thr Ile Val Asn Tyr Thr 770 775 780 Asn Glu Ser Ile Leu Asn Tyr Tyr Trp Asn Asn Ser Asn Ile Asn Asn 785 790 795 800 Phe Thr Ala Thr Cys Ile Ile Asn Asn Thr Ile Ser Ser Ser Asn Glu 805 810 815 Thr Glu Leu Ile Asn Cys Thr Asn Pro Thr Leu Lys Tyr Gln Asn Tyr 820 825 830 Phe Tyr Thr Phe Phe Lys Leu Phe Tyr Ile Ile Ile Phe Ile Val Ser 835 840 845 Gly Ile Ile Ile Ser Ile Leu Phe Ile Ser Ile Ile Thr Phe Leu Ser 850 855 860 Leu Arg Lys Arg Lys Lys His Val Glu Glu Ile Glu Ser Pro Pro Pro 865 870 875 880 Ser Glu Ser Asn Glu Glu Glu Gln Cys Gln His Asp Asp Thr Thr Ser 885 890 895 Ile His Glu Pro Ser Pro Arg Glu Pro Leu Leu Pro Lys Pro Tyr Ser 900 905 910 Arg Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr Met Arg Pro Ser Thr Gln 915 920 925 Pro Leu Asn Pro Phe Pro Leu Pro Lys Pro Cys Pro Pro Pro Lys Pro 930 935 940 Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Ser Ala 945 950 955 960 Glu Ser Tyr Ser Pro Pro Lys Pro Leu Pro Ser Ile Pro Leu Leu Pro 965 970 975 Asn Ile Pro Pro Leu Ser Thr Gln Asn Ile Ser Leu Ile His Val Asp 980 985 990 Arg Ile Ile 995 <210> 47 <211> 2068 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 3'' <400> 47 Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 1 5 10 15 Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu 20 25 30 Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 35 40 45 Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 50 55 60 Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Tyr His Asp Tyr 65 70 75 80 Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro Pro Leu Trp Glu 85 90 95 Ser Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn Ile Leu Asn Ser 100 105 110 Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val Tyr Asn Glu Tyr 115 120 125 Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln Leu Ser Asp Leu 130 135 140 Arg Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys Asn Ile Gly Tyr 145 150 155 160 Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile Pro Lys Asn Lys 165 170 175 Leu Phe Asn His Phe Lys Asn His Arg Pro Ala Phe Ser Thr His Thr 180 185 190 Glu Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu Lys Lys Thr Phe 195 200 205 Pro Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn Cys Leu Thr Gln 210 215 220 Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn Ser Ser Val Pro 225 230 235 240 Ser Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser Tyr Glu Asp Leu 245 250 255 Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu Gln His Leu Gly 260 265 270 Asp Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile Pro His Lys Asp 275 280 285 Val Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro Ala Ser Val Glu 290 295 300 Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn Pro His Val Lys 305 310 315 320 Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser Thr Tyr Thr Asp 325 330 335 Val Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser Gln His Gly Arg 340 345 350 Glu Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu Gln Val Ser Asn 355 360 365 Ile Leu His Ser Val Phe Ala Asn His Ser Ile Lys Pro Gln Ile Leu 370 375 380 Glu Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser Ile Lys Pro Val 385 390 395 400 Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr Met Glu Asn Ile 405 410 415 Val Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn Tyr Ser Ser Pro 420 425 430 Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala Glu Tyr Phe Asp 435 440 445 Thr Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr Ile Pro Lys Tyr 450 455 460 Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr Phe Pro Lys Asp 465 470 475 480 Phe Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu Lys Asp His Ser 485 490 495 Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser Ile Ser Pro Ser 500 505 510 Leu Arg Gly Arg Lys Arg Arg Ser Pro Ser Asn Met Lys Gln Phe Cys 515 520 525 Lys Ile Ser Val Trp Leu Gln Gln His Asp Pro Asp Leu Leu Glu Ile 530 535 540 Ile Asn Asn Leu Cys Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys 545 550 555 560 His Gly Val Thr Phe Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu 565 570 575 Ile Lys Lys His Ala Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr 580 585 590 Leu Glu Ser Leu Ile Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe 595 600 605 Thr Gly Glu Ile Gly Ser Tyr Thr Gly Val Lys Leu Glu Val Glu Lys 610 615 620 Thr Glu Ala Asn Lys Val Ile Leu Lys Asn Gly Glu Ala Val Leu Val 625 630 635 640 Pro Ala Ala Asp Phe Lys Pro Phe Pro Asp Arg Arg Leu Ala Val Trp 645 650 655 Ile Met Glu Ser Gly Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg 660 665 670 Lys Lys Glu Gly Gly Gly Asn Asp Pro Pro Val Pro Lys His Ile Ser 675 680 685 Pro Tyr Thr Pro Arg Thr Arg Ile Ala Ile Glu Val Glu Lys Ala Phe 690 695 700 Asp Asp Cys Met Arg Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu 705 710 715 720 Ala Lys Ser Val Ser Leu Leu Ser Phe Leu Ser Leu Asn His Pro Thr 725 730 735 Glu Phe Ile Lys Val Leu Pro Leu Ile Asp Phe Asp Pro Leu Val Thr 740 745 750 Phe Tyr Leu Leu Leu Glu Pro Tyr Lys Thr His Gly Asp Asp Phe Leu 755 760 765 Ile Pro Glu Thr Ile Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp 770 775 780 Leu Tyr Gln Ser Ala Met Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile 785 790 795 800 Thr Arg Gln Thr Phe Met Asp Ile Ala Asp Ser Ala Thr Lys Glu Val 805 810 815 Asp Val Pro Ile Cys Tyr Ser Asp Pro Glu Thr Val His Ser Tyr Ala 820 825 830 Asn His Val Arg Thr Glu Ile Leu His His Asn Ala Val Asn Lys Val 835 840 845 Thr Thr Pro Asn Leu Val Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr 850 855 860 Asn Thr Ile Arg His Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn 865 870 875 880 Ala Leu Arg Phe Trp Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe Val 885 890 895 Ile His Gly Leu His Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr 900 905 910 Ser Glu Phe Ala Glu Ile Val Arg Asn Leu Arg Phe Ser Arg Pro Gly 915 920 925 Asn Asn Tyr Ile Asn Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly 930 935 940 Asp Lys His Thr Thr Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala Met 945 950 955 960 Leu Val Ala Phe Ile Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro 965 970 975 Glu Glu Lys Val Gly Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp 980 985 990 Leu Val Pro Thr Arg Leu His Ser Phe Leu Asn His Asn Leu Ser Lys 995 1000 1005 Leu Lys Ile Leu Asn Arg Ala Gln Gln Thr Val Arg Asn Ile Leu Ser 1010 1015 1020 Asn Asp Cys Leu Asn Gln Leu Lys His Tyr Val Lys His Thr Gly Lys 1025 1030 1035 1040 Asn Glu Ile Leu Lys Leu Leu Gln Glu Arg Gly Arg Lys Arg Arg Ser 1045 1050 1055 Tyr Phe Leu Val Ala Asp His Arg Glu His His Val Ile Pro Phe Leu 1060 1065 1070 Lys Thr Asp Phe His His Met His Gln Asn Pro Ile Gln Lys Lys Gln 1075 1080 1085 Ala Leu Leu Glu Ile Lys Gln Leu Phe Thr Gly Asp Tyr Leu Ile Cys 1090 1095 1100 Lys Ser Pro Ser Thr Ile Leu Ala Cys Ile Glu Arg Lys Thr Tyr Lys 1105 1110 1115 1120 Asp Phe Ala Ala Ser Leu Lys Asp Gly Arg Tyr Lys Asn Arg Gln Lys 1125 1130 1135 Met Leu Ser Leu Arg Glu Gln Thr Asn Cys Gln Leu Tyr Phe Phe Val 1140 1145 1150 Glu Gly Pro Ala Phe Pro Asn Pro Gln Lys Lys Ile Asn His Val Ala 1155 1160 1165 Tyr Ala Ser Ile Ile Thr Ala Met Thr His Leu Met Val Arg Asp His 1170 1175 1180 Ile Phe Val Ile Gln Thr Lys Asn Glu Ala His Ser Ser Gln Lys Leu 1185 1190 1195 1200 Val Gln Leu Phe Tyr Ala Phe Ser Lys Glu Met Val Cys Val Val Pro 1205 1210 1215 Thr Ser Leu Thr Pro Thr Asp Glu Glu Leu Cys Ile Lys Leu Trp Ser 1220 1225 1230 Ser Leu Ser Gly Ile Ser Gly Val Ile Gly Lys Ile Leu Ala Asn Thr 1235 1240 1245 Cys Ser Val Ala His Leu Val Ser Gly Lys Leu Ser Ser Gln Asn Ile 1250 1255 1260 Asp Gln Leu Lys Thr Pro Ser Asn Arg Pro Phe Pro Lys Lys Val Lys 1265 1270 1275 1280 Arg Met Leu Ile Ser Ile Ser Lys Gly Asn Lys Glu Leu Glu Ile Lys 1285 1290 1295 Leu Leu Ser Gly Val Pro Asn Ile Gly Lys Lys Leu Ala Ala Glu Ile 1300 1305 1310 Leu Lys Asp His Ala Leu Leu Phe Phe Leu Asn Gln Pro Val Glu Cys 1315 1320 1325 Leu Ala Asn Ile Gln Ile Val Gln Lys Thr Arg Thr Ile Lys Leu Gly 1330 1335 1340 Met Lys Arg Ala Glu Ala Ile His Tyr Phe Leu Asn Trp Cys Gly Ser 1345 1350 1355 1360 Ala His Val Thr Asp Asp Ser Gln Asn Ile Thr Glu Ala Ser Arg Pro 1365 1370 1375 Thr Met Gln Val Ala Thr Gln Ser Ala Ala Thr Gln Pro Ala Ala Thr 1380 1385 1390 Gln Pro Leu His Glu Val Ser Asp Asp Ala Ser Ser Asp Ala Ser Ser 1395 1400 1405 Pro Val Gly His Gln Thr Leu Ser Lys Glu Met Leu Leu Asn Thr Ala 1410 1415 1420 Arg Gly Arg Lys Arg Arg Ser Val Glu Thr Gln Met Asp Lys Leu Gly 1425 1430 1435 1440 Phe Leu Leu Asn His Ile Gly Lys Gln Val Thr Thr Lys Val Leu Ser 1445 1450 1455 Asn Ala His Ile Thr Gln Thr Met Lys Glu Ile Ile Leu Glu Asn His 1460 1465 1470 Ser Val Asp Gly Gly Ala Ala Lys Asn Val Ser Lys Gly Lys Ser Ser 1475 1480 1485 Pro Lys Glu Lys Lys His Trp Thr Glu Phe Glu Ser Trp Glu Gln Leu 1490 1495 1500 Ser Lys Ser Lys Arg Ser Phe Lys Glu Tyr Trp Ala Glu Arg Asn Glu 1505 1510 1515 1520 Ile Val Asn Thr Leu Leu Leu Asn Trp Asp Asn Val Arg Gly Ala Ile 1525 1530 1535 Lys Lys Phe Leu Asp Asp Asp Arg Glu Trp Cys Gly Arg Ile Asn Met 1540 1545 1550 Ile Asn Gly Val Pro Glu Ile Val Glu Ile Ile Pro Ser Pro Tyr Arg 1555 1560 1565 Ala Gly Glu Asn Ile Tyr Phe Gly Ser Glu Ala Met Met Pro Ala Asp 1570 1575 1580 Ile Tyr Ser Arg Val Ala Asn Lys Pro Ala Met Phe Val Phe His Thr 1585 1590 1595 1600 His Pro Asn Leu Gly Ser Cys Cys Gly Gly Met Pro Ser Ile Cys Asp 1605 1610 1615 Ile Ser Thr Thr Leu Arg Tyr Leu Leu Met Gly Trp Thr Ala Gly His 1620 1625 1630 Leu Ile Ile Ser Ser Asn Gln Val Gly Met Leu Thr Val Asp Lys Arg 1635 1640 1645 Ile Ile Val Asp Leu Trp Ala Asn Glu Asn Pro Arg Trp Leu Met Ala 1650 1655 1660 Gln Lys Ile Leu Asp Ile Phe Met Met Leu Thr Ser Arg Arg Ser Leu 1665 1670 1675 1680 Val Asn Pro Trp Thr Leu Arg Asp Leu Lys Lys Ile Leu Gln Asp Tyr 1685 1690 1695 Gly Ile Glu Tyr Ile Ile Phe Pro Ser Asn Asp Phe Phe Ile Tyr Glu 1700 1705 1710 Asp Glu Arg Leu Leu Met Phe Ser Lys Lys Trp Thr Asn Phe Phe Thr 1715 1720 1725 Leu His Glu Leu Leu Asp Asp Leu Glu Thr Ile Glu Thr Lys Ala Ser 1730 1735 1740 Ser Thr Thr Arg Gly Arg Lys Arg Arg Ser Ser Thr Lys Lys Lys Pro 1745 1750 1755 1760 Thr Ile Thr Lys Gln Glu Leu Tyr Ser Leu Val Ala Ala Asp Thr Gln 1765 1770 1775 Leu Asn Lys Ala Leu Ile Glu Arg Ile Phe Thr Ser Gln Gln Lys Ile 1780 1785 1790 Ile Gln Asn Ala Leu Lys His Asn Gln Glu Val Ile Ile Pro Pro Gly 1795 1800 1805 Ile Lys Phe Thr Val Val Thr Val Lys Ala Lys Pro Ala Arg Gln Gly 1810 1815 1820 His Asn Pro Ala Thr Gly Glu Pro Ile Gln Ile Lys Ala Lys Pro Glu 1825 1830 1835 1840 His Lys Ala Val Lys Ile Arg Ala Leu Lys Pro Val His Asp Met Leu 1845 1850 1855 Asn Arg Gly Arg Lys Arg Arg Ser Val Glu Pro Arg Glu Gln Phe Phe 1860 1865 1870 Gln Asp Leu Leu Ser Ala Val Asp Gln Gln Met Asp Thr Val Lys Asn 1875 1880 1885 Asp Ile Lys Asp Ile Met Lys Glu Lys Thr Ser Phe Met Val Ser Phe 1890 1895 1900 Glu Asn Phe Ile Glu Arg Tyr Asp Thr Met Glu Lys Asn Ile Gln Asp 1905 1910 1915 1920 Leu Gln Asn Lys Tyr Glu Glu Met Ala Ala Asn Leu Met Thr Val Met 1925 1930 1935 Thr Asp Thr Lys Ile Gln Leu Gly Ala Ile Ile Ala Gln Leu Glu Ile 1940 1945 1950 Leu Met Ile Asn Gly Thr Pro Leu Pro Ala Lys Lys Thr Thr Ile Lys 1955 1960 1965 Glu Ala Met Pro Leu Pro Ser Ser Asn Thr Asn Asn Glu Gln Thr Ser 1970 1975 1980 Pro Pro Ala Ser Gly Lys Thr Ser Glu Thr Pro Lys Lys Asn Pro Thr 1985 1990 1995 2000 Asn Ala Met Phe Phe Thr Arg Ser Glu Trp Ala Ser Ser Asn Thr Phe 2005 2010 2015 Arg Glu Lys Phe Leu Thr Pro Glu Ile Gln Ala Ile Leu Asp Glu Gln 2020 2025 2030 Phe Ala Asn Lys Thr Gly Ile Glu Arg Leu His Ala Glu Gly Leu Tyr 2035 2040 2045 Met Trp Arg Thr Gln Phe Ser Asp Glu Gln Lys Lys Met Val Lys Glu 2050 2055 2060 Met Met Lys Lys 2065 <210> 48 <211> 1531 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 4'' <400> 48 Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 1 5 10 15 Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu 20 25 30 Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 35 40 45 Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 50 55 60 Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Asp Phe Ile Leu 65 70 75 80 Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser 85 90 95 Ser Ser Gln Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser 100 105 110 Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr 115 120 125 Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr 130 135 140 Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met 145 150 155 160 Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser 165 170 175 Glu Ser Ile His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser 180 185 190 Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Thr Pro Lys 195 200 205 Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu 210 215 220 Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn 225 230 235 240 Phe Ile Gln Thr Ile His Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu 245 250 255 Val Val Arg Leu Met Val Ile Lys Leu Leu Lys Lys Lys Arg Gly Arg 260 265 270 Lys Arg Arg Ser Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His 275 280 285 Tyr Gly Glu Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr 290 295 300 His Met Tyr Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile 305 310 315 320 Ile Val Leu Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala 325 330 335 Ala Ile Glu Glu Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln 340 345 350 Gln Trp Ala Glu Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly 355 360 365 Ala Thr Thr Ala Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr 370 375 380 Asn Arg Pro Ala Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp 385 390 395 400 Arg Leu Val Met Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala 405 410 415 Ser Ala Pro Ala His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln 420 425 430 Asn Thr Ala Ser Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg 435 440 445 Asn Thr Tyr Thr His Lys Asp Leu Glu Asn Ser Leu Arg Gly Arg Lys 450 455 460 Arg Arg Ser Pro Ser Asn Met Lys Gln Phe Cys Lys Ile Ser Val Trp 465 470 475 480 Leu Gln Gln His Asp Pro Asp Leu Leu Glu Ile Ile Asn Asn Leu Cys 485 490 495 Met Leu Gly Asn Leu Ser Ala Ala Lys Tyr Lys His Gly Val Thr Phe 500 505 510 Ile Tyr Pro Lys Gln Ala Lys Ile Arg Asp Glu Ile Lys Lys His Ala 515 520 525 Tyr Ser Asn Asp Pro Ser Gln Ala Ile Lys Thr Leu Glu Ser Leu Ile 530 535 540 Leu Pro Phe Tyr Ile Pro Thr Pro Ala Glu Phe Thr Gly Glu Ile Gly 545 550 555 560 Ser Tyr Thr Gly Val Lys Leu Glu Val Glu Lys Thr Glu Ala Asn Lys 565 570 575 Val Ile Leu Lys Asn Gly Glu Ala Val Leu Val Pro Ala Ala Asp Phe 580 585 590 Lys Pro Phe Pro Asp Arg Arg Leu Ala Val Trp Ile Met Glu Ser Gly 595 600 605 Ser Met Pro Leu Glu Gly Pro Pro Tyr Lys Arg Lys Lys Glu Gly Gly 610 615 620 Gly Asn Asp Pro Pro Val Pro Lys His Ile Ser Pro Tyr Thr Pro Arg 625 630 635 640 Thr Arg Ile Ala Ile Glu Val Glu Lys Ala Phe Asp Asp Cys Met Arg 645 650 655 Gln Asn Trp Cys Ser Val Asn Asn Pro Tyr Leu Ala Lys Ser Val Ser 660 665 670 Leu Leu Ser Phe Leu Ser Leu Asn His Pro Thr Glu Phe Ile Lys Val 675 680 685 Leu Pro Leu Ile Asp Phe Asp Pro Leu Val Thr Phe Tyr Leu Leu Leu 690 695 700 Glu Pro Tyr Lys Thr His Gly Asp Asp Phe Leu Ile Pro Glu Thr Ile 705 710 715 720 Leu Phe Gly Pro Thr Gly Trp Asn Gly Thr Asp Leu Tyr Gln Ser Ala 725 730 735 Met Leu Glu Phe Lys Lys Phe Phe Thr Gln Ile Thr Arg Gln Thr Phe 740 745 750 Met Asp Ile Ala Asp Ser Ala Thr Lys Glu Val Asp Val Pro Ile Cys 755 760 765 Tyr Ser Asp Pro Glu Thr Val His Ser Tyr Ala Asn His Val Arg Thr 770 775 780 Glu Ile Leu His His Asn Ala Val Asn Lys Val Thr Thr Pro Asn Leu 785 790 795 800 Val Val Gln Ala Tyr Asn Glu Leu Glu Gln Thr Asn Thr Ile Arg His 805 810 815 Tyr Gly Pro Ile Phe Pro Glu Ser Thr Ile Asn Ala Leu Arg Phe Trp 820 825 830 Lys Lys Leu Trp Gln Asp Glu Gln Arg Phe Val Ile His Gly Leu His 835 840 845 Arg Thr Leu Met Asp Gln Pro Thr Tyr Glu Thr Ser Glu Phe Ala Glu 850 855 860 Ile Val Arg Asn Leu Arg Phe Ser Arg Pro Gly Asn Asn Tyr Ile Asn 865 870 875 880 Glu Leu Asn Ile Thr Ser Pro Ala Met Tyr Gly Asp Lys His Thr Thr 885 890 895 Gly Asp Ile Ala Pro Asn Asp Arg Phe Ala Met Leu Val Ala Phe Ile 900 905 910 Asn Ser Thr Asp Phe Leu Tyr Thr Ala Ile Pro Glu Glu Lys Val Gly 915 920 925 Gly Asn Glu Thr Gln Thr Ser Ser Leu Thr Asp Leu Val Pro Thr Arg 930 935 940 Leu His Ser Phe Leu Asn His Asn Leu Ser Lys Leu Lys Ile Leu Asn 945 950 955 960 Arg Ala Gln Gln Thr Val Arg Asn Ile Leu Ser Asn Asp Cys Leu Asn 965 970 975 Gln Leu Lys His Tyr Val Lys His Thr Gly Lys Asn Glu Ile Leu Lys 980 985 990 Leu Leu Gln Glu Arg Gly Arg Lys Arg Arg Ser Phe Ser Lys Lys Lys 995 1000 1005 Tyr Ile Gly Leu Ile Asp Lys Lys Cys Glu Leu Lys Lys Ile Leu Asp 1010 1015 1020 Asp Tyr Ser Ile Leu Ile Ile Gly Ile Leu Ile Gly Thr Asn Ile Ile 1025 1030 1035 1040 Thr Leu Ile Tyr Asn Phe Ile Gly Trp Ile Asn Tyr Ile Lys Cys Tyr 1045 1050 1055 Gln Asn Asp Asp Lys Met Phe Tyr Cys Pro Lys Asp Trp Val Gly Tyr 1060 1065 1070 Asn Asn Val Cys Tyr Tyr Phe Ser Asn Glu Glu Lys Asn Tyr Thr Asn 1075 1080 1085 Ala Ser Asn Tyr Cys Lys Gln Leu Asn Ser Thr Leu Ala Asn Asn Asn 1090 1095 1100 Thr Asn Leu Val Asn Leu Thr Lys Thr Leu Asn Leu Thr Lys Thr Tyr 1105 1110 1115 1120 Asn His Glu Ser Asn Tyr Trp Val Asn Tyr Ser Leu Ile Lys Asn Glu 1125 1130 1135 Ser Val Leu Leu Arg Asn Ser Gly Tyr Tyr Lys Lys Gln Lys His Val 1140 1145 1150 Ser Leu Leu Tyr Ile Cys Ser Lys Arg Gly Arg Lys Arg Arg Ser Ile 1155 1160 1165 Ile Leu Ile Phe Leu Ile Phe Ser Asn Ile Val Leu Ser Ile Ile Tyr 1170 1175 1180 Trp Val Ser Phe Asn Lys Thr Ile Ile Leu Asp Ser Asn Ile Thr Asn 1185 1190 1195 1200 Asp Asn Asn Asp Ile Asn Gly Val Ser Trp Asn Thr Tyr Phe Asn Asn 1205 1210 1215 Ser Phe Asn Thr Leu Ala Thr Cys Gly Lys Ala Gly Asn Phe Cys Glu 1220 1225 1230 Cys Ser Asn Tyr Ser Thr Ser Leu Tyr Asn Ile Thr Asn Asn Cys Ser 1235 1240 1245 Leu Thr Ile Phe Pro His Asn Thr Asp Ile Phe Asn Thr Thr Tyr Gln 1250 1255 1260 Leu Val Tyr Leu Asn Gln Lys Ile Asn Tyr Thr Ile Lys Leu Leu Thr 1265 1270 1275 1280 Pro Val Asp Ser Pro Asn Ile Thr Tyr Asn Cys Thr Asn Ser Leu Ile 1285 1290 1295 Thr Cys Lys Lys Asn Asn Gly Thr Asn Thr Asn Ile Tyr Leu Asn Ile 1300 1305 1310 Asn Asp Thr Ile Val Asn Tyr Thr Asn Glu Ser Ile Leu Asn Tyr Tyr 1315 1320 1325 Trp Asn Asn Ser Asn Ile Asn Asn Phe Thr Ala Thr Cys Ile Ile Asn 1330 1335 1340 Asn Thr Ile Ser Ser Ser Asn Glu Thr Glu Leu Ile Asn Cys Thr Asn 1345 1350 1355 1360 Pro Thr Leu Lys Tyr Gln Asn Tyr Phe Tyr Thr Phe Phe Lys Leu Phe 1365 1370 1375 Tyr Ile Ile Ile Phe Ile Val Ser Gly Ile Ile Ile Ser Ile Leu Phe 1380 1385 1390 Ile Ser Ile Ile Thr Phe Leu Ser Leu Arg Lys Arg Lys Lys His Val 1395 1400 1405 Glu Glu Ile Glu Ser Pro Pro Pro Ser Glu Ser Asn Glu Glu Glu Gln 1410 1415 1420 Cys Gln His Asp Asp Thr Thr Ser Ile His Glu Pro Ser Pro Arg Glu 1425 1430 1435 1440 Pro Leu Leu Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn Thr Pro Ile 1445 1450 1455 Tyr Tyr Met Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe Pro Leu Pro 1460 1465 1470 Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro 1475 1480 1485 Pro Pro Lys Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro Pro Lys Pro 1490 1495 1500 Leu Pro Ser Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu Ser Thr Gln 1505 1510 1515 1520 Asn Ile Ser Leu Ile His Val Asp Arg Ile Ile 1525 1530 <210> 49 <211> 1532 <212> PRT <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 5'' <400> 49 Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 1 5 10 15 Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Gly Lys Ile Gln Glu 20 25 30 Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 35 40 45 Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 50 55 60 Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Ala Tyr His Asp Tyr 65 70 75 80 Ala Ser Lys Leu Leu Ala Asp Tyr Arg Ser Asp Pro Pro Leu Trp Glu 85 90 95 Ser Asp Leu Pro Arg His Asn Arg Tyr Ser Asp Asn Ile Leu Asn Ser 100 105 110 Arg Tyr Cys Gly Asn Lys Asn Gly Ala Ala Pro Val Tyr Asn Glu Tyr 115 120 125 Thr Asn Ser Pro Glu Lys Ala Glu Lys Gly Leu Gln Leu Ser Asp Leu 130 135 140 Arg Asn Phe Ser Phe Met Leu Asn Pro Gln His Lys Asn Ile Gly Tyr 145 150 155 160 Gly Asp Ala Gln Asp Leu Glu Pro Tyr Ser Ser Ile Pro Lys Asn Lys 165 170 175 Leu Phe Asn His Phe Lys Asn His Arg Pro Ala Phe Ser Thr His Thr 180 185 190 Glu Asn Leu Ile Arg Arg Asn Val Val Arg Thr Glu Lys Lys Thr Phe 195 200 205 Pro Gln Val Ala Ser Leu Lys Gly Thr Gln Lys Asn Cys Leu Thr Gln 210 215 220 Pro Ser Ser Leu Pro Ser Leu Lys Asn Pro Lys Asn Ser Ser Val Pro 225 230 235 240 Ser Thr Arg Phe Ser Glu His Thr Lys Phe Phe Ser Tyr Glu Asp Leu 245 250 255 Pro Lys Leu Arg Thr Lys Gly Thr Ile Lys His Glu Gln His Leu Gly 260 265 270 Asp Gln Met Pro Gly Gln His Tyr Asn Gly Tyr Ile Pro His Lys Asp 275 280 285 Val Tyr Asn Ile Leu Cys Leu Ala His Asn Leu Pro Ala Ser Val Glu 290 295 300 Lys Gly Ile Ala Gly Arg Gly Ile Pro Leu Gly Asn Pro His Val Lys 305 310 315 320 Pro Asn Ile Glu Gln Glu Leu Ile Lys Ser Thr Ser Thr Tyr Thr Asp 325 330 335 Val Pro Met Leu Gly Pro Leu Pro Pro Lys Asp Ser Gln His Gly Arg 340 345 350 Glu Tyr Gln Glu Phe Ser Ala Asn Arg His Met Leu Gln Val Ser Asn 355 360 365 Ile Leu His Ser Val Phe Ala Asn His Ser Ile Lys Pro Gln Ile Leu 370 375 380 Glu Asp Ile Pro Val Leu Asn Ala Gln Leu Thr Ser Ile Lys Pro Val 385 390 395 400 Ser Pro Phe Leu Asn Lys Ala Tyr Gln Thr His Tyr Met Glu Asn Ile 405 410 415 Val Thr Leu Val Pro Arg Phe Lys Ser Ile Ala Asn Tyr Ser Ser Pro 420 425 430 Ile Pro Asn Tyr Ser Lys Arg Asn Ser Gly Gln Ala Glu Tyr Phe Asp 435 440 445 Thr Ser Lys Gln Thr Ile Ser Arg His Asn Asn Tyr Ile Pro Lys Tyr 450 455 460 Thr Gly Gly Ile Gly Asp Ser Lys Leu Asp Ser Thr Phe Pro Lys Asp 465 470 475 480 Phe Asn Ala Ser Ser Val Pro Leu Thr Ser Ala Glu Lys Asp His Ser 485 490 495 Leu Arg Gly Asp Asn Ser Ala Cys Cys Ile Ser Ser Ile Ser Pro Ser 500 505 510 Leu Arg Gly Arg Lys Arg Arg Ser Tyr Phe Leu Val Ala Asp His Arg 515 520 525 Glu His His Val Ile Pro Phe Leu Lys Thr Asp Phe His His Met His 530 535 540 Gln Asn Pro Ile Gln Lys Lys Gln Ala Leu Leu Glu Ile Lys Gln Leu 545 550 555 560 Phe Thr Gly Asp Tyr Leu Ile Cys Lys Ser Pro Ser Thr Ile Leu Ala 565 570 575 Cys Ile Glu Arg Lys Thr Tyr Lys Asp Phe Ala Ala Ser Leu Lys Asp 580 585 590 Gly Arg Tyr Lys Asn Arg Gln Lys Met Leu Ser Leu Arg Glu Gln Thr 595 600 605 Asn Cys Gln Leu Tyr Phe Phe Val Glu Gly Pro Ala Phe Pro Asn Pro 610 615 620 Gln Lys Lys Ile Asn His Val Ala Tyr Ala Ser Ile Ile Thr Ala Met 625 630 635 640 Thr His Leu Met Val Arg Asp His Ile Phe Val Ile Gln Thr Lys Asn 645 650 655 Glu Ala His Ser Ser Gln Lys Leu Val Gln Leu Phe Tyr Ala Phe Ser 660 665 670 Lys Glu Met Val Cys Val Val Pro Thr Ser Leu Thr Pro Thr Asp Glu 675 680 685 Glu Leu Cys Ile Lys Leu Trp Ser Ser Leu Ser Gly Ile Ser Gly Val 690 695 700 Ile Gly Lys Ile Leu Ala Asn Thr Cys Ser Val Ala His Leu Val Ser 705 710 715 720 Gly Lys Leu Ser Ser Gln Asn Ile Asp Gln Leu Lys Thr Pro Ser Asn 725 730 735 Arg Pro Phe Pro Lys Lys Val Lys Arg Met Leu Ile Ser Ile Ser Lys 740 745 750 Gly Asn Lys Glu Leu Glu Ile Lys Leu Leu Ser Gly Val Pro Asn Ile 755 760 765 Gly Lys Lys Leu Ala Ala Glu Ile Leu Lys Asp His Ala Leu Leu Phe 770 775 780 Phe Leu Asn Gln Pro Val Glu Cys Leu Ala Asn Ile Gln Ile Val Gln 785 790 795 800 Lys Thr Arg Thr Ile Lys Leu Gly Met Lys Arg Ala Glu Ala Ile His 805 810 815 Tyr Phe Leu Asn Trp Cys Gly Ser Ala His Val Thr Asp Asp Ser Gln 820 825 830 Asn Ile Thr Glu Ala Ser Arg Pro Thr Met Gln Val Ala Thr Gln Ser 835 840 845 Ala Ala Thr Gln Pro Ala Ala Thr Gln Pro Leu His Glu Val Ser Asp 850 855 860 Asp Ala Ser Ser Asp Ala Ser Ser Pro Val Gly His Gln Thr Leu Ser 865 870 875 880 Lys Glu Met Leu Leu Asn Thr Ala Arg Gly Arg Lys Arg Arg Ser Val 885 890 895 Glu Thr Gln Met Asp Lys Leu Gly Phe Leu Leu Asn His Ile Gly Lys 900 905 910 Gln Val Thr Thr Lys Val Leu Ser Asn Ala His Ile Thr Gln Thr Met 915 920 925 Lys Glu Ile Ile Leu Glu Asn His Ser Val Asp Gly Gly Ala Ala Lys 930 935 940 Asn Val Ser Lys Gly Lys Ser Ser Pro Lys Glu Lys Lys His Trp Thr 945 950 955 960 Glu Phe Glu Ser Trp Glu Gln Leu Ser Lys Ser Lys Arg Ser Phe Lys 965 970 975 Glu Tyr Trp Ala Glu Arg Asn Glu Ile Val Asn Thr Leu Leu Leu Asn 980 985 990 Trp Asp Asn Val Arg Gly Ala Ile Lys Lys Phe Leu Asp Asp Asp Arg 995 1000 1005 Glu Trp Cys Gly Arg Ile Asn Met Ile Asn Gly Val Pro Glu Ile Val 1010 1015 1020 Glu Ile Ile Pro Ser Pro Tyr Arg Ala Gly Glu Asn Ile Tyr Phe Gly 1025 1030 1035 1040 Ser Glu Ala Met Met Pro Ala Asp Ile Tyr Ser Arg Val Ala Asn Lys 1045 1050 1055 Pro Ala Met Phe Val Phe His Thr His Pro Asn Leu Gly Ser Cys Cys 1060 1065 1070 Gly Gly Met Pro Ser Ile Cys Asp Ile Ser Thr Thr Leu Arg Tyr Leu 1075 1080 1085 Leu Met Gly Trp Thr Ala Gly His Leu Ile Ile Ser Ser Asn Gln Val 1090 1095 1100 Gly Met Leu Thr Val Asp Lys Arg Ile Ile Val Asp Leu Trp Ala Asn 1105 1110 1115 1120 Glu Asn Pro Arg Trp Leu Met Ala Gln Lys Ile Leu Asp Ile Phe Met 1125 1130 1135 Met Leu Thr Ser Arg Arg Ser Leu Val Asn Pro Trp Thr Leu Arg Asp 1140 1145 1150 Leu Lys Lys Ile Leu Gln Asp Tyr Gly Ile Glu Tyr Ile Ile Phe Pro 1155 1160 1165 Ser Asn Asp Phe Phe Ile Tyr Glu Asp Glu Arg Leu Leu Met Phe Ser 1170 1175 1180 Lys Lys Trp Thr Asn Phe Phe Thr Leu His Glu Leu Leu Asp Asp Leu 1185 1190 1195 1200 Glu Thr Ile Glu Thr Lys Ala Ser Ser Thr Thr Arg Gly Arg Lys Arg 1205 1210 1215 Arg Ser Ser Thr Lys Lys Lys Pro Thr Ile Thr Lys Gln Glu Leu Tyr 1220 1225 1230 Ser Leu Val Ala Ala Asp Thr Gln Leu Asn Lys Ala Leu Ile Glu Arg 1235 1240 1245 Ile Phe Thr Ser Gln Gln Lys Ile Ile Gln Asn Ala Leu Lys His Asn 1250 1255 1260 Gln Glu Val Ile Ile Pro Pro Gly Ile Lys Phe Thr Val Val Thr Val 1265 1270 1275 1280 Lys Ala Lys Pro Ala Arg Gln Gly His Asn Pro Ala Thr Gly Glu Pro 1285 1290 1295 Ile Gln Ile Lys Ala Lys Pro Glu His Lys Ala Val Lys Ile Arg Ala 1300 1305 1310 Leu Lys Pro Val His Asp Met Leu Asn Arg Gly Arg Lys Arg Arg Ser 1315 1320 1325 Val Glu Pro Arg Glu Gln Phe Phe Gln Asp Leu Leu Ser Ala Val Asp 1330 1335 1340 Gln Gln Met Asp Thr Val Lys Asn Asp Ile Lys Asp Ile Met Lys Glu 1345 1350 1355 1360 Lys Thr Ser Phe Met Val Ser Phe Glu Asn Phe Ile Glu Arg Tyr Asp 1365 1370 1375 Thr Met Glu Lys Asn Ile Gln Asp Leu Gln Asn Lys Tyr Glu Glu Met 1380 1385 1390 Ala Ala Asn Leu Met Thr Val Met Thr Asp Thr Lys Ile Gln Leu Gly 1395 1400 1405 Ala Ile Ile Ala Gln Leu Glu Ile Leu Met Ile Asn Gly Thr Pro Leu 1410 1415 1420 Pro Ala Lys Lys Thr Thr Ile Lys Glu Ala Met Pro Leu Pro Ser Ser 1425 1430 1435 1440 Asn Thr Asn Asn Glu Gln Thr Ser Pro Pro Ala Ser Gly Lys Thr Ser 1445 1450 1455 Glu Thr Pro Lys Lys Asn Pro Thr Asn Ala Met Phe Phe Thr Arg Ser 1460 1465 1470 Glu Trp Ala Ser Ser Asn Thr Phe Arg Glu Lys Phe Leu Thr Pro Glu 1475 1480 1485 Ile Gln Ala Ile Leu Asp Glu Gln Phe Ala Asn Lys Thr Gly Ile Glu 1490 1495 1500 Arg Leu His Ala Glu Gly Leu Tyr Met Trp Arg Thr Gln Phe Ser Asp 1505 1510 1515 1520 Glu Gln Lys Lys Met Val Lys Glu Met Met Lys Lys 1525 1530 <210> 50 <211> 8985 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 1'' <400> 50 atgcagatct ttgtaaagac cttgactggt aagaccatca ccctggaagt ggagcccagt 60 gacaccattg agaatgtcaa ggggaagatt caagaaaaag agggcatccc cccagaccag 120 cagaggctga tctttgccgg gaagcagctg gaagatgggc gcaccctgtc tgactacaac 180 atccagaagg agtccaccct gcacctggtc ctccgcctca gaggtgccga ttttatttta 240 aatatatcca tgaaaatgga ggtcatcttc aaaacggatt taagatcatc ttcacaagtt 300 gtgtttcatg cgggtagcct gtataattgg ttttctgttg agattatcaa tagcggtaga 360 attgttacga ccgctataaa aacattgctt agtactgtta agtatgatat tgtgaaatct 420 gctcgtatat atgcagggca agggtatact gaacatcagg ctcaagaaga atggaatatg 480 attctgcatg tgctgtttga agaggagacg gaatcctcag catcttcgga gtcaattcat 540 gaaaaaaatg ataatgaaac caatgaatgc acatcctcct ttgaaacgtt gtttgagcaa 600 gagccctcat cggagacacc taaagactcc aagctgtata tgcttgcaca aaagactgtg 660 caacatattg aacaatatgg aaaggcacct gattttaaca aggttattag agcacataat 720 tttattcaaa ccattcatgg aacccctcta aaggaagaag aaaaagaggt ggtaagactc 780 atggttatta aacttttaaa aaaaaaaaga ggaagaaaga gaagatcaga ttctgaattt 840 tttcaaccgg tttatccgcg gcattatggt gagtgtttgt caccagtcac tacaccaagc 900 ttcttctcca cacatatgta tactattctc attgctatcg tggtcttagt catcattatc 960 atcgttctaa tctatctatt ctcttcaaga aagaaaaaag ctgctgctgc tattgaggag 1020 gaagatatac agtttataaa tccttatcaa gatcagcagt gggcagaagt cactccacaa 1080 ccaggtacct ctaaaccagc tggagcgact acagcaagtg taggcaagcc agtcacgggc 1140 agaccggcaa caaacagacc agcaacaaac aaaccagtta cggacaaccc agttacggac 1200 agactagtca tggcaactgg cgggccggcg gccgcacctg cggccgcgag tgctcctgct 1260 catccggctg agccttacac gacagtcact actcagaaca ctgcttcaca aacaatgtcg 1320 gctattgaaa atttacgaca aagaaacacc tatacgcata aagacctaga aaactccttg 1380 agaggaagaa agagaagatc attttctaag aaaaagtaca tcggtcttat cgataagaag 1440 tgtgaattga aaaaaatact agatgattat agtatattaa taattggaat attaattgga 1500 actaacatca taacccttat ttataatttt ataggatgga ttaattatat aaaatgttac 1560 caaaatgatg ataagatgtt ttattgccct aaagattggg ttggatataa taatgtttgt 1620 tattatttta gtaatgaaga aaaaaattat actaatgcaa gtaattattg taagcaatta 1680 aatagtacgc ttgctaataa taatactaat ttagtaaatc ttactaaaac attaaatctt 1740 actaaaacat ataatcacga atctaattat tgggttaatt attctttaat taaaaatgag 1800 tcagtactat tacgtaatag tggatattac aaaaaacaaa aacatgtaag tttattatat 1860 atttgtagta aaagaggaag aaagagaaga tcaataatac ttattttttt aatattttct 1920 aacatagttt taagtattat atattgggtt agttttaata aaacaataat tttagatagt 1980 aatattacta atgataataa tgatataaat ggagtatcat ggaatactta ttttaataat 2040 tcttttaata cactagctac atgtggaaaa gcaggtaact tttgtgaatg ttctaattat 2100 agtacatcac tatataatat aacaaataat tgtagcttaa ctatttttcc tcataatact 2160 gatatattta atacaacata tcaactagta tatctaaatc aaaaaattaa ttatacaata 2220 aaattattaa cacctgtaga ttctccaaat atcacatata attgtactaa ttctttaata 2280 acatgtaaaa aaaataatgg aacaaacact aatatatatt taaatataaa tgatactata 2340 gttaattata ctaatgaaag tatacttaat tattattgga ataatagtaa cattaacaat 2400 tttacagcta catgtataat taataataca attagttctt ctaatgaaac agaacttata 2460 aattgtacta atcccacatt gaaatatcaa aactattttt atactttttt taaattattt 2520 tatattataa tatttatcgt atctgggata ataataagta ttctttttat atccatcata 2580 acttttttat ctttacgaaa aagaaaaaaa catgttgaag aaatagaaag tccaccacct 2640 tctgaatcta atgaagaaga acaatgtcag catgatgaca ccacttccat acatgaacca 2700 tctcccagag aaccattact tcctaagcct tacagtcgtt atcagtataa tacacctatt 2760 tactacatgc gtccctcaac acaaccactc aacccatttc ccttacctaa accgtgtcct 2820 ccacccaaac catgtccgcc acccaaacca tgtcctccac ctaaaccatg tccttcagct 2880 gaatcctatt ctccacccaa accactacct agtatcccgc tactacccaa tatcccgcca 2940 ttatctaccc aaaatatttc gcttattcac gtagatagaa ttattagagg aagaaagaga 3000 agatcatatc atgattatgc ttcaaagctg ttagccgatt ataggtcaga tccacctctt 3060 tgggaaagcg atttgccgcg tcataaccgg tattctgata atatactaaa ttcccggtac 3120 tgcggcaaca aaaatggagc tgcgcctgta tacaatgaat acactaatag tcctgaaaag 3180 gctgaaaagg gattgcaact cagtgatcta cgtaactttt cttttatgct gaaccctcag 3240 cacaaaaaca ttggttacgg cgatgcgcaa gatctggagc cgtacagctc cattccaaaa 3300 aataaacttt ttaatcattt taaaaatcat cgtcctgctt tttcaaccca tacggaaaac 3360 ctaattaggc gcaatgttgt tcgtacggaa aaaaaaacat ttccgcaggt ggctagctta 3420 aagggcacac agaaaaattg tctgacccag ccttcctcat tgccttcctt aaaaaaccca 3480 aaaaatagtt cggttccttc tacgaggttc agcgagcaca caaaattttt ttcgtacgag 3540 gatcttccta aactcagaac aaagggtacc attaagcatg agcagcacct tggcgatcag 3600 atgccgggcc agcactataa cggatatatt ccgcacaaag acgtgtacaa tattttgtgt 3660 ttagcgcaca atctacctgc gtcggtagaa aaagggattg caggtagagg tattccttta 3720 ggaaatccac atgtcaaacc gaacatagag caggaactca taaaatccac cagtacctac 3780 acggacgtgc cgatgcttgg tccccttccg cccaaagatt cgcaacacgg cagagaatac 3840 caggaatttt ccgcaaatag acatatgcta caagtatcta atatactaca ttctgtgttt 3900 gctaatcatt ctataaagcc acaaatccta gaagacatac cggtactcaa tgcacaacta 3960 acaagcataa agccggtaag cccattttta aacaaagcgt atcaaaccca ttatatggaa 4020 aatattgtta ccttggtgcc ccggtttaag agtattgcca actactcaag ccctataccg 4080 aattattcta aaagaaatag cggtcaggct gaatattttg atacctcaaa gcaaacaatt 4140 tctagacaca ataattatat acctaaatat acgggaggaa ttggagattc caaactagac 4200 agcacctttc ctaaagattt caacgcctcc tcggtaccgt taacaagcgc ggaaaaggac 4260 cattccctgc ggggagacaa ctcagcatgt tgcatctcat ctatatctcc atcattgaga 4320 ggaagaaaga gaagatcacc ctctaatatg aaacagtttt gcaagatttc tgtatggcta 4380 cagcagcacg atccagattt attagaaatt atcaacaact tatgtatgct tggcaattta 4440 tccgcggcaa agtacaaaca cggagttacc ttcatttacc ccaaacaggc aaagatccgc 4500 gatgaaataa aaaaacatgc ctactccaat gacccttcac aagccataaa gaccttagaa 4560 tcactcatcc ttccatttta cattcccact ccagcggagt tcaccgggga aatcggctcc 4620 tacaccggag tgaaattaga ggttgaaaaa acggaggcga ataaagttat tttaaaaaat 4680 ggagaagcgg tcctagtacc ggcggccgat tttaagccct ttcctgatcg ccgactagcg 4740 gtctggatca tggagtcagg ctctatgccc ctggagggtc ccccctataa gcggaaaaag 4800 gagggtgggg ggaatgaccc gccggttcct aagcatatct cgccgtatac tccgcgcacg 4860 cgtattgcca ttgaggtgga aaaggccttt gatgactgta tgcgtcaaaa ctggtgtagt 4920 gtcaataatc cctatcttgc caagtcggtc tccttgctgt ctttcttgtc gctcaaccat 4980 cccaccgagt ttattaaggt actgccgctt atagactttg accccttggt gaccttttat 5040 ctacttcttg agccctataa aacgcatggg gatgactttt taattccgga aaccatttta 5100 ttcggcccta ccggatggaa tggtacagat ctgtatcaaa gtgccatgct ggagtttaaa 5160 aagtttttta cccagattac tcgccaaacc tttatggaca tagccgattc ggctactaag 5220 gaggtagatg ttcccatatg ttactcggat cccgaaaccg tacattccta tgccaatcac 5280 gtgcgtactg aaattttgca tcacaatgcc gtcaataagg ttacaacacc taacctcgtc 5340 gtgcaggcct ataatgagct cgagcaaacc aataccatac gacattacgg ccctattttc 5400 ccggaaagta ccatcaacgc actgcgtttt tggaaaaagc tgtggcagga tgaacagcga 5460 tttgttatcc acggcctgca ccgcacgttg atggatcaac ccacctatga aacctctgag 5520 tttgcagaga tcgttagaaa tttacggttt tcgcgtcccg gcaataacta tataaacgag 5580 cttaatatta caagtcccgc tatgtacggc gacaagcata ccaccggaga tattgcgccc 5640 aatgatagat ttgccatgtt ggtggccttt atcaacagta ctgacttttt atacaccgcg 5700 attcccgagg aaaaggtagg ggggaatgaa acccaaacca gtagccttac agacctagtt 5760 ccaacacggc tacactcttt tttaaatcat aatctaagca aacttaaaat cttaaaccgc 5820 gcgcagcaaa cggttagaaa tattctttca aatgattgtc ttaatcaact gaaacattat 5880 gttaaacaca cgggaaaaaa tgaaatacta aagttacttc aagaaagagg aagaaagaga 5940 agatcatatt ttctagtagc agatcatcga gaacatcatg tgattccttt tcttaaaacc 6000 gatttccatc acatgcatca aaatcctata caaaaaaaac aagctctcct agaaatcaaa 6060 cagcttttta ctggagatta tctcatctgc aaaagccctt ctaccattct ggcctgtatt 6120 gaacgaaaaa cctacaaaga ctttgcggct tctttgaaag atggacgtta taaaaatcgc 6180 caaaaaatgc tgtcgctgcg agaacaaacc aactgtcaac tttatttttt tgtagaaggc 6240 ccggcatttc ctaaccctca aaaaaaaatt aatcacgttg cctatgcaag cattattact 6300 gctatgacgc atcttatggt tagagatcat atttttgtca ttcaaacgaa aaatgaggcc 6360 cacagttccc aaaagcttgt gcagcttttt tatgcctttt ctaaggaaat ggtgtgcgtc 6420 gttcccacct ccctcacccc cacggatgaa gagctatgca tcaagctatg gtcttctctt 6480 tctggtattt caggcgtgat aggtaaaatc ttggcaaaca cttgttccgt agctcatttg 6540 gtttctggaa agctttcatc gcagaatatt gatcagttaa aaactccctc caaccgacca 6600 ttccccaaaa aagtaaaacg tatgcttata agcattagca aaggaaataa ggagttagaa 6660 ataaaattgc tctcgggggt tcccaatatc gggaaaaaat tagctgccga aattttaaaa 6720 gatcatgcgc ttcttttttt tctaaatcag cccgtagaat gcttggcaaa tatacaaatc 6780 gttcaaaaaa cccgtacgat taagttggga atgaagcgag ccgaagcgat tcattatttt 6840 ttaaactggt gtggctctgc ccatgtaacc gatgatagcc aaaatatcac agaggcgtcg 6900 cggcctacaa tgcaggtcgc gacgcagtcc gccgcaactc agcccgctgc aacgcagcca 6960 ttgcacgaag tatcagatga tgcatcatca gatgcttcat cacccgtagg gcatcaaaca 7020 ttatctaaag aaatgttatt gaacacagcc agaggaagaa agagaagatc agttgagaca 7080 caaatggaca aacttggttt tctgctaaat cacataggta aacaggttac cactaaggtg 7140 cttagcaatg cccatataac tcaaacgatg aaggagatta ttttggaaaa tcatagtgta 7200 gatggtggag ccgcaaaaaa tgtttcaaaa gggaagtctt ccccaaaaga aaaaaaacat 7260 tggactgagt tcgaatcctg ggaacagctc agcaagtcta aaagaagttt taaggaatac 7320 tgggcggagc gtaatgagat tgtgaatact ctgttgctta actgggacaa tgttcggggt 7380 gccatcaaaa aatttttgga cgatgaccgt gaatggtgcg gccgcattaa tatgataaac 7440 ggtgtacccg agatagtgga aatcattcca agcccctata gggcaggaga gaacatttat 7500 tttggcagcg aggctatgat gcctgctgat atttatagca gggtggccaa caagcctgct 7560 atgtttgtgt ttcacacgca tcctaatttg ggttcatgtt gtggaggaat gccttccata 7620 tgtgacattt ctacaacgct gcgttatcta ctcatggggt ggactgctgg gcatctaatc 7680 atttcttcga atcaagtagg aatgctcacg gttgataaga gaattattgt tgatttgtgg 7740 gccaatgaga atccgcgctg gcttatggcg caaaaaatat tagatatttt tatgatgctc 7800 acttcgcgta gaagcctggt aaatccctgg accctgagag acctaaaaaa aatattacaa 7860 gactatggta ttgagtatat catttttcct tcgaatgact tttttattta tgaagacgaa 7920 cgtcttttaa tgttttcaaa aaaatggacc aactttttta cgttacatga gttattggat 7980 gacctcgaaa ctattgagac aaaggcatcg tccacaacaa gaggaagaaa gagaagatca 8040 tcgacaaaaa aaaagcccac aattaccaag caagagcttt actccttagt agcggcagat 8100 acccagttaa ataaagcatt gattgaaaga atctttacaa gtcagcaaaa aataatacaa 8160 aatgctttaa agcacaatca agaagttatt ataccacccg gaatcaagtt caccgtcgtt 8220 acggtgaaag ctaaacctgc tcgccagggc cataatcccg ccacaggaga gcctattcaa 8280 attaaagcta aacctgaaca taaagccgta aagatacgag cattgaaacc tgtccatgat 8340 atgttaaaca gaggaagaaa gagaagatca gttgagccac gcgaacagtt ttttcaagat 8400 ctgctttcag cagtggatca acaaatggac actgtaaaaa atgacataaa agacattatg 8460 aaagaaaaaa cgtcttttat ggtatcattc gaaaacttta tagaacgtta cgataccatg 8520 gaaaaaaata ttcaagacct tcagaataag tacgaagaaa tggcggccaa ccttatgacc 8580 gtcatgacgg atacaaaaat tcagcttgga gccattatcg cccaacttga gattctaatg 8640 ataaatggca ctccacttcc ggcaaaaaag acaacaatta aggaggctat gcccttacct 8700 tcatcaaaca cgaataatga acaaacgagt cctcccgcct caggcaaaac aagtgaaaca 8760 cctaaaaaaa atcccacgaa tgcgatgttc ttcacgcgta gcgaatgggc atcctcgaat 8820 acttttcgag aaaagttttt aacaccagaa attcaagcca tattggatga gcagtttgca 8880 aacaagaccg ggatcgaaag attgcatgcc gagggtcttt acatgtggag aacccaattc 8940 tctgacgaac agaagaaaat ggtcaaagag atgatgaaga agtaa 8985 <210> 51 <211> 2988 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 2'' <400> 51 atgcagatct ttgtaaagac cttgactggt aagaccatca ccctggaagt ggagcccagt 60 gacaccattg agaatgtcaa ggggaagatt caagaaaaag agggcatccc cccagaccag 120 cagaggctga tctttgccgg gaagcagctg gaagatgggc gcaccctgtc tgactacaac 180 atccagaagg agtccaccct gcacctggtc ctccgcctca gaggtgccga ttttatttta 240 aatatatcca tgaaaatgga ggtcatcttc aaaacggatt taagatcatc ttcacaagtt 300 gtgtttcatg cgggtagcct gtataattgg ttttctgttg agattatcaa tagcggtaga 360 attgttacga ccgctataaa aacattgctt agtactgtta agtatgatat tgtgaaatct 420 gctcgtatat atgcagggca agggtatact gaacatcagg ctcaagaaga atggaatatg 480 attctgcatg tgctgtttga agaggagacg gaatcctcag catcttcgga gtcaattcat 540 gaaaaaaatg ataatgaaac caatgaatgc acatcctcct ttgaaacgtt gtttgagcaa 600 gagccctcat cggagacacc taaagactcc aagctgtata tgcttgcaca aaagactgtg 660 caacatattg aacaatatgg aaaggcacct gattttaaca aggttattag agcacataat 720 tttattcaaa ccattcatgg aacccctcta aaggaagaag aaaaagaggt ggtaagactc 780 atggttatta aacttttaaa aaaaaaaaga ggaagaaaga gaagatcaga ttctgaattt 840 tttcaaccgg tttatccgcg gcattatggt gagtgtttgt caccagtcac tacaccaagc 900 ttcttctcca cacatatgta tactattctc attgctatcg tggtcttagt catcattatc 960 atcgttctaa tctatctatt ctcttcaaga aagaaaaaag ctgctgctgc tattgaggag 1020 gaagatatac agtttataaa tccttatcaa gatcagcagt gggcagaagt cactccacaa 1080 ccaggtacct ctaaaccagc tggagcgact acagcaagtg taggcaagcc agtcacgggc 1140 agaccggcaa caaacagacc agcaacaaac aaaccagtta cggacaaccc agttacggac 1200 agactagtca tggcaactgg cgggccggcg gccgcacctg cggccgcgag tgctcctgct 1260 catccggctg agccttacac gacagtcact actcagaaca ctgcttcaca aacaatgtcg 1320 gctattgaaa atttacgaca aagaaacacc tatacgcata aagacctaga aaactccttg 1380 agaggaagaa agagaagatc attttctaag aaaaagtaca tcggtcttat cgataagaag 1440 tgtgaattga aaaaaatact agatgattat agtatattaa taattggaat attaattgga 1500 actaacatca taacccttat ttataatttt ataggatgga ttaattatat aaaatgttac 1560 caaaatgatg ataagatgtt ttattgccct aaagattggg ttggatataa taatgtttgt 1620 tattatttta gtaatgaaga aaaaaattat actaatgcaa gtaattattg taagcaatta 1680 aatagtacgc ttgctaataa taatactaat ttagtaaatc ttactaaaac attaaatctt 1740 actaaaacat ataatcacga atctaattat tgggttaatt attctttaat taaaaatgag 1800 tcagtactat tacgtaatag tggatattac aaaaaacaaa aacatgtaag tttattatat 1860 atttgtagta aaagaggaag aaagagaaga tcaataatac ttattttttt aatattttct 1920 aacatagttt taagtattat atattgggtt agttttaata aaacaataat tttagatagt 1980 aatattacta atgataataa tgatataaat ggagtatcat ggaatactta ttttaataat 2040 tcttttaata cactagctac atgtggaaaa gcaggtaact tttgtgaatg ttctaattat 2100 agtacatcac tatataatat aacaaataat tgtagcttaa ctatttttcc tcataatact 2160 gatatattta atacaacata tcaactagta tatctaaatc aaaaaattaa ttatacaata 2220 aaattattaa cacctgtaga ttctccaaat atcacatata attgtactaa ttctttaata 2280 acatgtaaaa aaaataatgg aacaaacact aatatatatt taaatataaa tgatactata 2340 gttaattata ctaatgaaag tatacttaat tattattgga ataatagtaa cattaacaat 2400 tttacagcta catgtataat taataataca attagttctt ctaatgaaac agaacttata 2460 aattgtacta atcccacatt gaaatatcaa aactattttt atactttttt taaattattt 2520 tatattataa tatttatcgt atctgggata ataataagta ttctttttat atccatcata 2580 acttttttat ctttacgaaa aagaaaaaaa catgttgaag aaatagaaag tccaccacct 2640 tctgaatcta atgaagaaga acaatgtcag catgatgaca ccacttccat acatgaacca 2700 tctcccagag aaccattact tcctaagcct tacagtcgtt atcagtataa tacacctatt 2760 tactacatgc gtccctcaac acaaccactc aacccatttc ccttacctaa accgtgtcct 2820 ccacccaaac catgtccgcc acccaaacca tgtcctccac ctaaaccatg tccttcagct 2880 gaatcctatt ctccacccaa accactacct agtatcccgc tactacccaa tatcccgcca 2940 ttatctaccc aaaatatttc gcttattcac gtagatagaa ttatttaa 2988 <210> 52 <211> 6207 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 3'' <400> 52 atgcagatct ttgtaaagac cttgactggt aagaccatca ccctggaagt ggagcccagt 60 gacaccattg agaatgtcaa ggggaagatt caagaaaaag agggcatccc cccagaccag 120 cagaggctga tctttgccgg gaagcagctg gaagatgggc gcaccctgtc tgactacaac 180 atccagaagg agtccaccct gcacctggtc ctccgcctca gaggtgccta tcatgattat 240 gcttcaaagc tgttagccga ttataggtca gatccacctc tttgggaaag cgatttgccg 300 cgtcataacc ggtattctga taatatacta aattcccggt actgcggcaa caaaaatgga 360 gctgcgcctg tatacaatga atacactaat agtcctgaaa aggctgaaaa gggattgcaa 420 ctcagtgatc tacgtaactt ttcttttatg ctgaaccctc agcacaaaaa cattggttac 480 ggcgatgcgc aagatctgga gccgtacagc tccattccaa aaaataaact ttttaatcat 540 tttaaaaatc atcgtcctgc tttttcaacc catacggaaa acctaattag gcgcaatgtt 600 gttcgtacgg aaaaaaaaac atttccgcag gtggctagct taaagggcac acagaaaaat 660 tgtctgaccc agccttcctc attgccttcc ttaaaaaacc caaaaaatag ttcggttcct 720 tctacgaggt tcagcgagca cacaaaattt ttttcgtacg aggatcttcc taaactcaga 780 acaaagggta ccattaagca tgagcagcac cttggcgatc agatgccggg ccagcactat 840 aacggatata ttccgcacaa agacgtgtac aatattttgt gtttagcgca caatctacct 900 gcgtcggtag aaaaagggat tgcaggtaga ggtattcctt taggaaatcc acatgtcaaa 960 ccgaacatag agcaggaact cataaaatcc accagtacct acacggacgt gccgatgctt 1020 ggtccccttc cgcccaaaga ttcgcaacac ggcagagaat accaggaatt ttccgcaaat 1080 agacatatgc tacaagtatc taatatacta cattctgtgt ttgctaatca ttctataaag 1140 ccacaaatcc tagaagacat accggtactc aatgcacaac taacaagcat aaagccggta 1200 agcccatttt taaacaaagc gtatcaaacc cattatatgg aaaatattgt taccttggtg 1260 ccccggttta agagtattgc caactactca agccctatac cgaattattc taaaagaaat 1320 agcggtcagg ctgaatattt tgatacctca aagcaaacaa tttctagaca caataattat 1380 atacctaaat atacgggagg aattggagat tccaaactag acagcacctt tcctaaagat 1440 ttcaacgcct cctcggtacc gttaacaagc gcggaaaagg accattccct gcggggagac 1500 aactcagcat gttgcatctc atctatatct ccatcattga gaggaagaaa gagaagatca 1560 ccctctaata tgaaacagtt ttgcaagatt tctgtatggc tacagcagca cgatccagat 1620 ttattagaaa ttatcaacaa cttatgtatg cttggcaatt tatccgcggc aaagtacaaa 1680 cacggagtta ccttcattta ccccaaacag gcaaagatcc gcgatgaaat aaaaaaacat 1740 gcctactcca atgacccttc acaagccata aagaccttag aatcactcat ccttccattt 1800 tacattccca ctccagcgga gttcaccggg gaaatcggct cctacaccgg agtgaaatta 1860 gaggttgaaa aaacggaggc gaataaagtt attttaaaaa atggagaagc ggtcctagta 1920 ccggcggccg attttaagcc ctttcctgat cgccgactag cggtctggat catggagtca 1980 ggctctatgc ccctggaggg tcccccctat aagcggaaaa aggagggtgg ggggaatgac 2040 ccgccggttc ctaagcatat ctcgccgtat actccgcgca cgcgtattgc cattgaggtg 2100 gaaaaggcct ttgatgactg tatgcgtcaa aactggtgta gtgtcaataa tccctatctt 2160 gccaagtcgg tctccttgct gtctttcttg tcgctcaacc atcccaccga gtttattaag 2220 gtactgccgc ttatagactt tgaccccttg gtgacctttt atctacttct tgagccctat 2280 aaaacgcatg gggatgactt tttaattccg gaaaccattt tattcggccc taccggatgg 2340 aatggtacag atctgtatca aagtgccatg ctggagttta aaaagttttt tacccagatt 2400 actcgccaaa cctttatgga catagccgat tcggctacta aggaggtaga tgttcccata 2460 tgttactcgg atcccgaaac cgtacattcc tatgccaatc acgtgcgtac tgaaattttg 2520 catcacaatg ccgtcaataa ggttacaaca cctaacctcg tcgtgcaggc ctataatgag 2580 ctcgagcaaa ccaataccat acgacattac ggccctattt tcccggaaag taccatcaac 2640 gcactgcgtt tttggaaaaa gctgtggcag gatgaacagc gatttgttat ccacggcctg 2700 caccgcacgt tgatggatca acccacctat gaaacctctg agtttgcaga gatcgttaga 2760 aatttacggt tttcgcgtcc cggcaataac tatataaacg agcttaatat tacaagtccc 2820 gctatgtacg gcgacaagca taccaccgga gatattgcgc ccaatgatag atttgccatg 2880 ttggtggcct ttatcaacag tactgacttt ttatacaccg cgattcccga ggaaaaggta 2940 ggggggaatg aaacccaaac cagtagcctt acagacctag ttccaacacg gctacactct 3000 tttttaaatc ataatctaag caaacttaaa atcttaaacc gcgcgcagca aacggttaga 3060 aatattcttt caaatgattg tcttaatcaa ctgaaacatt atgttaaaca cacgggaaaa 3120 aatgaaatac taaagttact tcaagaaaga ggaagaaaga gaagatcata ttttctagta 3180 gcagatcatc gagaacatca tgtgattcct tttcttaaaa ccgatttcca tcacatgcat 3240 caaaatccta tacaaaaaaa acaagctctc ctagaaatca aacagctttt tactggagat 3300 tatctcatct gcaaaagccc ttctaccatt ctggcctgta ttgaacgaaa aacctacaaa 3360 gactttgcgg cttctttgaa agatggacgt tataaaaatc gccaaaaaat gctgtcgctg 3420 cgagaacaaa ccaactgtca actttatttt tttgtagaag gcccggcatt tcctaaccct 3480 caaaaaaaaa ttaatcacgt tgcctatgca agcattatta ctgctatgac gcatcttatg 3540 gttagagatc atatttttgt cattcaaacg aaaaatgagg cccacagttc ccaaaagctt 3600 gtgcagcttt tttatgcctt ttctaaggaa atggtgtgcg tcgttcccac ctccctcacc 3660 cccacggatg aagagctatg catcaagcta tggtcttctc tttctggtat ttcaggcgtg 3720 ataggtaaaa tcttggcaaa cacttgttcc gtagctcatt tggtttctgg aaagctttca 3780 tcgcagaata ttgatcagtt aaaaactccc tccaaccgac cattccccaa aaaagtaaaa 3840 cgtatgctta taagcattag caaaggaaat aaggagttag aaataaaatt gctctcgggg 3900 gttcccaata tcgggaaaaa attagctgcc gaaattttaa aagatcatgc gcttcttttt 3960 tttctaaatc agcccgtaga atgcttggca aatatacaaa tcgttcaaaa aacccgtacg 4020 attaagttgg gaatgaagcg agccgaagcg attcattatt ttttaaactg gtgtggctct 4080 gcccatgtaa ccgatgatag ccaaaatatc acagaggcgt cgcggcctac aatgcaggtc 4140 gcgacgcagt ccgccgcaac tcagcccgct gcaacgcagc cattgcacga agtatcagat 4200 gatgcatcat cagatgcttc atcacccgta gggcatcaaa cattatctaa agaaatgtta 4260 ttgaacacag ccagaggaag aaagagaaga tcagttgaga cacaaatgga caaacttggt 4320 tttctgctaa atcacatagg taaacaggtt accactaagg tgcttagcaa tgcccatata 4380 actcaaacga tgaaggagat tattttggaa aatcatagtg tagatggtgg agccgcaaaa 4440 aatgtttcaa aagggaagtc ttccccaaaa gaaaaaaaac attggactga gttcgaatcc 4500 tgggaacagc tcagcaagtc taaaagaagt tttaaggaat actgggcgga gcgtaatgag 4560 attgtgaata ctctgttgct taactgggac aatgttcggg gtgccatcaa aaaatttttg 4620 gacgatgacc gtgaatggtg cggccgcatt aatatgataa acggtgtacc cgagatagtg 4680 gaaatcattc caagccccta tagggcagga gagaacattt attttggcag cgaggctatg 4740 atgcctgctg atatttatag cagggtggcc aacaagcctg ctatgtttgt gtttcacacg 4800 catcctaatt tgggttcatg ttgtggagga atgccttcca tatgtgacat ttctacaacg 4860 ctgcgttatc tactcatggg gtggactgct gggcatctaa tcatttcttc gaatcaagta 4920 ggaatgctca cggttgataa gagaattatt gttgatttgt gggccaatga gaatccgcgc 4980 tggcttatgg cgcaaaaaat attagatatt tttatgatgc tcacttcgcg tagaagcctg 5040 gtaaatccct ggaccctgag agacctaaaa aaaatattac aagactatgg tattgagtat 5100 atcatttttc cttcgaatga cttttttatt tatgaagacg aacgtctttt aatgttttca 5160 aaaaaatgga ccaacttttt tacgttacat gagttattgg atgacctcga aactattgag 5220 acaaaggcat cgtccacaac aagaggaaga aagagaagat catcgacaaa aaaaaagccc 5280 acaattacca agcaagagct ttactcctta gtagcggcag atacccagtt aaataaagca 5340 ttgattgaaa gaatctttac aagtcagcaa aaaataatac aaaatgcttt aaagcacaat 5400 caagaagtta ttataccacc cggaatcaag ttcaccgtcg ttacggtgaa agctaaacct 5460 gctcgccagg gccataatcc cgccacagga gagcctattc aaattaaagc taaacctgaa 5520 cataaagccg taaagatacg agcattgaaa cctgtccatg atatgttaaa cagaggaaga 5580 aagagaagat cagttgagcc acgcgaacag ttttttcaag atctgctttc agcagtggat 5640 caacaaatgg acactgtaaa aaatgacata aaagacatta tgaaagaaaa aacgtctttt 5700 atggtatcat tcgaaaactt tatagaacgt tacgatacca tggaaaaaaa tattcaagac 5760 cttcagaata agtacgaaga aatggcggcc aaccttatga ccgtcatgac ggatacaaaa 5820 attcagcttg gagccattat cgcccaactt gagattctaa tgataaatgg cactccactt 5880 ccggcaaaaa agacaacaat taaggaggct atgcccttac cttcatcaaa cacgaataat 5940 gaacaaacga gtcctcccgc ctcaggcaaa acaagtgaaa cacctaaaaa aaatcccacg 6000 aatgcgatgt tcttcacgcg tagcgaatgg gcatcctcga atacttttcg agaaaagttt 6060 ttaacaccag aaattcaagc catattggat gagcagtttg caaacaagac cgggatcgaa 6120 agattgcatg ccgagggtct ttacatgtgg agaacccaat tctctgacga acagaagaaa 6180 atggtcaaag agatgatgaa gaagtaa 6207 <210> 53 <211> 4596 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 4'' <400> 53 atgcagatct ttgtaaagac cttgactggt aagaccatca ccctggaagt ggagcccagt 60 gacaccattg agaatgtcaa ggggaagatt caagaaaaag agggcatccc cccagaccag 120 cagaggctga tctttgccgg gaagcagctg gaagatgggc gcaccctgtc tgactacaac 180 atccagaagg agtccaccct gcacctggtc ctccgcctca gaggtgccga ttttatttta 240 aatatatcca tgaaaatgga ggtcatcttc aaaacggatt taagatcatc ttcacaagtt 300 gtgtttcatg cgggtagcct gtataattgg ttttctgttg agattatcaa tagcggtaga 360 attgttacga ccgctataaa aacattgctt agtactgtta agtatgatat tgtgaaatct 420 gctcgtatat atgcagggca agggtatact gaacatcagg ctcaagaaga atggaatatg 480 attctgcatg tgctgtttga agaggagacg gaatcctcag catcttcgga gtcaattcat 540 gaaaaaaatg ataatgaaac caatgaatgc acatcctcct ttgaaacgtt gtttgagcaa 600 gagccctcat cggagacacc taaagactcc aagctgtata tgcttgcaca aaagactgtg 660 caacatattg aacaatatgg aaaggcacct gattttaaca aggttattag agcacataat 720 tttattcaaa ccattcatgg aacccctcta aaggaagaag aaaaagaggt ggtaagactc 780 atggttatta aacttttaaa aaaaaaaaga ggaagaaaga gaagatcaga ttctgaattt 840 tttcaaccgg tttatccgcg gcattatggt gagtgtttgt caccagtcac tacaccaagc 900 ttcttctcca cacatatgta tactattctc attgctatcg tggtcttagt catcattatc 960 atcgttctaa tctatctatt ctcttcaaga aagaaaaaag ctgctgctgc tattgaggag 1020 gaagatatac agtttataaa tccttatcaa gatcagcagt gggcagaagt cactccacaa 1080 ccaggtacct ctaaaccagc tggagcgact acagcaagtg taggcaagcc agtcacgggc 1140 agaccggcaa caaacagacc agcaacaaac aaaccagtta cggacaaccc agttacggac 1200 agactagtca tggcaactgg cgggccggcg gccgcacctg cggccgcgag tgctcctgct 1260 catccggctg agccttacac gacagtcact actcagaaca ctgcttcaca aacaatgtcg 1320 gctattgaaa atttacgaca aagaaacacc tatacgcata aagacctaga aaactccttg 1380 agaggaagaa agagaagatc accctctaat atgaaacagt tttgcaagat ttctgtatgg 1440 ctacagcagc acgatccaga tttattagaa attatcaaca acttatgtat gcttggcaat 1500 ttatccgcgg caaagtacaa acacggagtt accttcattt accccaaaca ggcaaagatc 1560 cgcgatgaaa taaaaaaaca tgcctactcc aatgaccctt cacaagccat aaagacctta 1620 gaatcactca tccttccatt ttacattccc actccagcgg agttcaccgg ggaaatcggc 1680 tcctacaccg gagtgaaatt agaggttgaa aaaacggagg cgaataaagt tattttaaaa 1740 aatggagaag cggtcctagt accggcggcc gattttaagc cctttcctga tcgccgacta 1800 gcggtctgga tcatggagtc aggctctatg cccctggagg gtccccccta taagcggaaa 1860 aaggagggtg gggggaatga cccgccggtt cctaagcata tctcgccgta tactccgcgc 1920 acgcgtattg ccattgaggt ggaaaaggcc tttgatgact gtatgcgtca aaactggtgt 1980 agtgtcaata atccctatct tgccaagtcg gtctccttgc tgtctttctt gtcgctcaac 2040 catcccaccg agtttattaa ggtactgccg cttatagact ttgacccctt ggtgaccttt 2100 tatctacttc ttgagcccta taaaacgcat ggggatgact ttttaattcc ggaaaccatt 2160 ttattcggcc ctaccggatg gaatggtaca gatctgtatc aaagtgccat gctggagttt 2220 aaaaagtttt ttacccagat tactcgccaa acctttatgg acatagccga ttcggctact 2280 aaggaggtag atgttcccat atgttactcg gatcccgaaa ccgtacattc ctatgccaat 2340 cacgtgcgta ctgaaatttt gcatcacaat gccgtcaata aggttacaac acctaacctc 2400 gtcgtgcagg cctataatga gctcgagcaa accaatacca tacgacatta cggccctatt 2460 ttcccggaaa gtaccatcaa cgcactgcgt ttttggaaaa agctgtggca ggatgaacag 2520 cgatttgtta tccacggcct gcaccgcacg ttgatggatc aacccaccta tgaaacctct 2580 gagtttgcag agatcgttag aaatttacgg ttttcgcgtc ccggcaataa ctatataaac 2640 gagcttaata ttacaagtcc cgctatgtac ggcgacaagc ataccaccgg agatattgcg 2700 cccaatgata gatttgccat gttggtggcc tttatcaaca gtactgactt tttatacacc 2760 gcgattcccg aggaaaaggt aggggggaat gaaacccaaa ccagtagcct tacagaccta 2820 gttccaacac ggctacactc ttttttaaat cataatctaa gcaaacttaa aatcttaaac 2880 cgcgcgcagc aaacggttag aaatattctt tcaaatgatt gtcttaatca actgaaacat 2940 tatgttaaac acacgggaaa aaatgaaata ctaaagttac ttcaagaaag aggaagaaag 3000 agaagatcat tttctaagaa aaagtacatc ggtcttatcg ataagaagtg tgaattgaaa 3060 aaaatactag atgattatag tatattaata attggaatat taattggaac taacatcata 3120 acccttattt ataattttat aggatggatt aattatataa aatgttacca aaatgatgat 3180 aagatgtttt attgccctaa agattgggtt ggatataata atgtttgtta ttattttagt 3240 aatgaagaaa aaaattatac taatgcaagt aattattgta agcaattaaa tagtacgctt 3300 gctaataata atactaattt agtaaatctt actaaaacat taaatcttac taaaacatat 3360 aatcacgaat ctaattattg ggttaattat tctttaatta aaaatgagtc agtactatta 3420 cgtaatagtg gatattacaa aaaacaaaaa catgtaagtt tattatatat ttgtagtaaa 3480 agaggaagaa agagaagatc aataatactt atttttttaa tattttctaa catagtttta 3540 agtattatat attgggttag ttttaataaa acaataattt tagatagtaa tattactaat 3600 gataataatg atataaatgg agtatcatgg aatacttatt ttaataattc ttttaataca 3660 ctagctacat gtggaaaagc aggtaacttt tgtgaatgtt ctaattatag tacatcacta 3720 tataatataa caaataattg tagcttaact atttttcctc ataatactga tatatttaat 3780 acaacatatc aactagtata tctaaatcaa aaaattaatt atacaataaa attattaaca 3840 cctgtagatt ctccaaatat cacatataat tgtactaatt ctttaataac atgtaaaaaa 3900 aataatggaa caaacactaa tatatattta aatataaatg atactatagt taattatact 3960 aatgaaagta tacttaatta ttattggaat aatagtaaca ttaacaattt tacagctaca 4020 tgtataatta ataatacaat tagttcttct aatgaaacag aacttataaa ttgtactaat 4080 cccacattga aatatcaaaa ctatttttat acttttttta aattatttta tattataata 4140 tttatcgtat ctgggataat aataagtatt ctttttatat ccatcataac ttttttatct 4200 ttacgaaaaa gaaaaaaaca tgttgaagaa atagaaagtc caccaccttc tgaatctaat 4260 gaagaagaac aatgtcagca tgatgacacc acttccatac atgaaccatc tcccagagaa 4320 ccattacttc ctaagcctta cagtcgttat cagtataata cacctattta ctacatgcgt 4380 ccctcaacac aaccactcaa cccatttccc ttacctaaac cgtgtcctcc acccaaacca 4440 tgtccgccac ccaaaccatg tcctccacct aaaccatgtc cttcagctga atcctattct 4500 ccacccaaac cactacctag tatcccgcta ctacccaata tcccgccatt atctacccaa 4560 aatatttcgc ttattcacgt agatagaatt atttaa 4596 <210> 54 <211> 4599 <212> DNA <213> Artificial Sequence <220> <223> PLS-ASF fusion protein no. 5'' <400> 54 atgcagatct ttgtaaagac cttgactggt aagaccatca ccctggaagt ggagcccagt 60 gacaccattg agaatgtcaa ggggaagatt caagaaaaag agggcatccc cccagaccag 120 cagaggctga tctttgccgg gaagcagctg gaagatgggc gcaccctgtc tgactacaac 180 atccagaagg agtccaccct gcacctggtc ctccgcctca gaggtgccta tcatgattat 240 gcttcaaagc tgttagccga ttataggtca gatccacctc tttgggaaag cgatttgccg 300 cgtcataacc ggtattctga taatatacta aattcccggt actgcggcaa caaaaatgga 360 gctgcgcctg tatacaatga atacactaat agtcctgaaa aggctgaaaa gggattgcaa 420 ctcagtgatc tacgtaactt ttcttttatg ctgaaccctc agcacaaaaa cattggttac 480 ggcgatgcgc aagatctgga gccgtacagc tccattccaa aaaataaact ttttaatcat 540 tttaaaaatc atcgtcctgc tttttcaacc catacggaaa acctaattag gcgcaatgtt 600 gttcgtacgg aaaaaaaaac atttccgcag gtggctagct taaagggcac acagaaaaat 660 tgtctgaccc agccttcctc attgccttcc ttaaaaaacc caaaaaatag ttcggttcct 720 tctacgaggt tcagcgagca cacaaaattt ttttcgtacg aggatcttcc taaactcaga 780 acaaagggta ccattaagca tgagcagcac cttggcgatc agatgccggg ccagcactat 840 aacggatata ttccgcacaa agacgtgtac aatattttgt gtttagcgca caatctacct 900 gcgtcggtag aaaaagggat tgcaggtaga ggtattcctt taggaaatcc acatgtcaaa 960 ccgaacatag agcaggaact cataaaatcc accagtacct acacggacgt gccgatgctt 1020 ggtccccttc cgcccaaaga ttcgcaacac ggcagagaat accaggaatt ttccgcaaat 1080 agacatatgc tacaagtatc taatatacta cattctgtgt ttgctaatca ttctataaag 1140 ccacaaatcc tagaagacat accggtactc aatgcacaac taacaagcat aaagccggta 1200 agcccatttt taaacaaagc gtatcaaacc cattatatgg aaaatattgt taccttggtg 1260 ccccggttta agagtattgc caactactca agccctatac cgaattattc taaaagaaat 1320 agcggtcagg ctgaatattt tgatacctca aagcaaacaa tttctagaca caataattat 1380 atacctaaat atacgggagg aattggagat tccaaactag acagcacctt tcctaaagat 1440 ttcaacgcct cctcggtacc gttaacaagc gcggaaaagg accattccct gcggggagac 1500 aactcagcat gttgcatctc atctatatct ccatcattga gaggaagaaa gagaagatca 1560 tattttctag tagcagatca tcgagaacat catgtgattc cttttcttaa aaccgatttc 1620 catcacatgc atcaaaatcc tatacaaaaa aaacaagctc tcctagaaat caaacagctt 1680 tttactggag attatctcat ctgcaaaagc ccttctacca ttctggcctg tattgaacga 1740 aaaacctaca aagactttgc ggcttctttg aaagatggac gttataaaaa tcgccaaaaa 1800 atgctgtcgc tgcgagaaca aaccaactgt caactttatt tttttgtaga aggcccggca 1860 tttcctaacc ctcaaaaaaa aattaatcac gttgcctatg caagcattat tactgctatg 1920 acgcatctta tggttagaga tcatattttt gtcattcaaa cgaaaaatga ggcccacagt 1980 tcccaaaagc ttgtgcagct tttttatgcc ttttctaagg aaatggtgtg cgtcgttccc 2040 acctccctca cccccacgga tgaagagcta tgcatcaagc tatggtcttc tctttctggt 2100 atttcaggcg tgataggtaa aatcttggca aacacttgtt ccgtagctca tttggtttct 2160 ggaaagcttt catcgcagaa tattgatcag ttaaaaactc cctccaaccg accattcccc 2220 aaaaaagtaa aacgtatgct tataagcatt agcaaaggaa ataaggagtt agaaataaaa 2280 ttgctctcgg gggttcccaa tatcgggaaa aaattagctg ccgaaatttt aaaagatcat 2340 gcgcttcttt tttttctaaa tcagcccgta gaatgcttgg caaatataca aatcgttcaa 2400 aaaacccgta cgattaagtt gggaatgaag cgagccgaag cgattcatta ttttttaaac 2460 tggtgtggct ctgcccatgt aaccgatgat agccaaaata tcacagaggc gtcgcggcct 2520 acaatgcagg tcgcgacgca gtccgccgca actcagcccg ctgcaacgca gccattgcac 2580 gaagtatcag atgatgcatc atcagatgct tcatcacccg tagggcatca aacattatct 2640 aaagaaatgt tattgaacac agccagagga agaaagagaa gatcagttga gacacaaatg 2700 gacaaacttg gttttctgct aaatcacata ggtaaacagg ttaccactaa ggtgcttagc 2760 aatgcccata taactcaaac gatgaaggag attattttgg aaaatcatag tgtagatggt 2820 ggagccgcaa aaaatgtttc aaaagggaag tcttccccaa aagaaaaaaa acattggact 2880 gagttcgaat cctgggaaca gctcagcaag tctaaaagaa gttttaagga atactgggcg 2940 gagcgtaatg agattgtgaa tactctgttg cttaactggg acaatgttcg gggtgccatc 3000 aaaaaatttt tggacgatga ccgtgaatgg tgcggccgca ttaatatgat aaacggtgta 3060 cccgagatag tggaaatcat tccaagcccc tatagggcag gagagaacat ttattttggc 3120 agcgaggcta tgatgcctgc tgatatttat agcagggtgg ccaacaagcc tgctatgttt 3180 gtgtttcaca cgcatcctaa tttgggttca tgttgtggag gaatgccttc catatgtgac 3240 atttctacaa cgctgcgtta tctactcatg gggtggactg ctgggcatct aatcatttct 3300 tcgaatcaag taggaatgct cacggttgat aagagaatta ttgttgattt gtgggccaat 3360 gagaatccgc gctggcttat ggcgcaaaaa atattagata tttttatgat gctcacttcg 3420 cgtagaagcc tggtaaatcc ctggaccctg agagacctaa aaaaaatatt acaagactat 3480 ggtattgagt atatcatttt tccttcgaat gactttttta tttatgaaga cgaacgtctt 3540 ttaatgtttt caaaaaaatg gaccaacttt tttacgttac atgagttatt ggatgacctc 3600 gaaactattg agacaaaggc atcgtccaca acaagaggaa gaaagagaag atcatcgaca 3660 aaaaaaaagc ccacaattac caagcaagag ctttactcct tagtagcggc agatacccag 3720 ttaaataaag cattgattga aagaatcttt acaagtcagc aaaaaataat acaaaatgct 3780 ttaaagcaca atcaagaagt tattatacca cccggaatca agttcaccgt cgttacggtg 3840 aaagctaaac ctgctcgcca gggccataat cccgccacag gagagcctat tcaaattaaa 3900 gctaaacctg aacataaagc cgtaaagata cgagcattga aacctgtcca tgatatgtta 3960 aacagaggaa gaaagagaag atcagttgag ccacgcgaac agttttttca agatctgctt 4020 tcagcagtgg atcaacaaat ggacactgta aaaaatgaca taaaagacat tatgaaagaa 4080 aaaacgtctt ttatggtatc attcgaaaac tttatagaac gttacgatac catggaaaaa 4140 aatattcaag accttcagaa taagtacgaa gaaatggcgg ccaaccttat gaccgtcatg 4200 acggatacaa aaattcagct tggagccatt atcgcccaac ttgagattct aatgataaat 4260 ggcactccac ttccggcaaa aaagacaaca attaaggagg ctatgccctt accttcatca 4320 aacacgaata atgaacaaac gagtcctccc gcctcaggca aaacaagtga aacacctaaa 4380 aaaaatccca cgaatgcgat gttcttcacg cgtagcgaat gggcatcctc gaatactttt 4440 cgagaaaagt ttttaacacc agaaattcaa gccatattgg atgagcagtt tgcaaacaag 4500 accgggatcg aaagattgca tgccgagggt ctttacatgt ggagaaccca attctctgac 4560 gaacagaaga aaatggtcaa agagatgatg aagaagtaa 4599

Claims (18)

  1. 서열번호 11 내지 20의 염기서열; 또는 서열번호 1 내지 10의 아미노산 서열을 코딩하는 염기서열;을 포함하는 폴리뉴클레오티드를 포함하는, 아프리카돼지열병용 DNA 백신 조성물.
  2. 제1항에 있어서,
    상기 폴리뉴클레오티드는 Kozak 서열, IgE leader 서열, 유비퀴틴(ubiquitin) 서열 및 절단 부위(cleavage site) 서열 중 하나 이상을 더 포함하는 것인, 조성물.
  3. 제1항에 있어서,
    상기 폴리뉴클레오티드는 서열번호 42의 염기서열을 더 포함하는 것인, 조성물.
  4. 제1항에 있어서,
    상기 폴리뉴클레오티드는 서열번호 44의 염기서열을 더 포함하는 것인, 조성물.
  5. 제1항에 있어서,
    상기 조성물은 상기 폴리뉴클레오티드를 포함하는 플라스미드를 포함하는 것인, 조성물.
  6. 제1항의 백신 조성물을 인간을 제외한 동물에게 투여하는 단계를 포함하는, 상기 동물에서 아프리카돼지열병 바이러스에 대한 면역 반응을 생성하는 방법.
  7. 제6항에 있어서,
    상기 투여는 전기천공(electroporation)에 의한 투여인 것을 특징으로 하는 방법.
  8. 제1항의 백신 조성물을 인간을 제외한 동물에게 투여하는 단계를 포함하는, 아프리카돼지열병을 치료 또는 예방하는 방법.
  9. 제8항에 있어서,
    상기 투여는 전기천공(electroporation)에 의한 투여인 것을 특징으로 하는 방법.
  10. 서열번호 11 내지 20의 염기서열; 또는 서열번호 1 내지 10의 아미노산 서열을 코딩하는 염기서열;을 포함하는 폴리뉴클레오티드를 포함하는, 아프리카돼지열병 치료 또는 예방용 약학 조성물.
  11. 삭제
  12. 삭제
  13. 삭제
  14. 삭제
  15. 삭제
  16. 삭제
  17. 삭제
  18. 삭제
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MX2022012925A (es) 2023-02-16
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