CN113151310B - 非洲猪瘟基因缺失弱毒株的构建及其作为疫苗的应用 - Google Patents

非洲猪瘟基因缺失弱毒株的构建及其作为疫苗的应用 Download PDF

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CN113151310B
CN113151310B CN202110176679.XA CN202110176679A CN113151310B CN 113151310 B CN113151310 B CN 113151310B CN 202110176679 A CN202110176679 A CN 202110176679A CN 113151310 B CN113151310 B CN 113151310B
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郑海学
李攀
冯涛
齐晓兰
刘华南
张克山
李丹
吴盼雪
马昭
党文
刘迎琦
石正旺
杨波
�田宏
郭建宏
刘湘涛
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Lanzhou Veterinary Research Institute of CAAS
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Abstract

本发明属于生物工程技术领域，具体涉及一种可用作疫苗的基因缺失减毒非洲猪瘟病毒株及其构建方法，该病毒株是亲本非洲猪瘟病毒ASFV CN/GS/2018分离株的基因缺失弱毒株。本发明发现了DP71L基因是对猪致病性致弱的新靶标基因，并发现联合丧失MGF360‑18R基因、DP71L基因和DP96R基因编码蛋白的功能，降低了亲本毒株的毒性，获得了一株安全性高的减毒非洲猪瘟候选疫苗株；所述减毒非洲猪瘟候选疫苗株高剂量(104HAD50)免疫猪后，对猪完全致弱，免疫猪健康，不发病，与免疫猪同居猪核酸也呈阴性，不水平传播，具有较好安全性；且能够对ASFV CN/GS/2018强毒株的攻毒提供100％免疫保护，可作为安全和有效的防控非洲猪瘟疫情的候选疫苗，具有极大的社会价值。

Description

非洲猪瘟基因缺失弱毒株的构建及其作为疫苗的应用
本申请要求申请日为2020年7月10日、申请号为CN202010661680.7、发明名称为“非洲猪瘟基因缺失弱毒株的构建及其作为疫苗的应用”的在先申请的优先权，该在先申请的全部内容均已在本申请中体现。
技术领域
本发明属于生物工程技术领域，具体涉及一种MGF360-18R基因、DP71L基因和DP96R 基因编码蛋白功能丧失的非洲猪瘟基因缺失弱毒株的构建及其作为疫苗的应用。
背景技术
非洲猪瘟(African Swine Fever，ASF)是由非洲猪瘟病毒(ASFV)感染引起的一种以猪发热和猪全身脏器出血为特征的急性烈性传染病，对于家猪的病死率高达100％。该病首先于1921在肯尼亚爆发，随后在整个非洲的家猪和野猪中广泛流行。20世纪50年代传入欧洲，整个欧洲用了40年消除该病。然而，该病自2007年再次从东非传入格鲁吉亚，随后便广泛在东欧散播，并于2017年传入俄罗斯远东地区伊尔库斯克。当前没有商品化的有效疫苗，非洲猪瘟疫情一旦发生，只能通过扑杀手段进行控制，但是这种方式不仅导致经济损失，而且需要花费漫长的时间，无法满足我国规模化养猪的需求。因此，疫苗作为预防和控制病毒传染病最有效、最经济的手段，对于我国规模养猪模式下的非洲猪瘟的防治至关重要。但非洲猪瘟病毒(ASFV)结构复杂、基因组庞大，大部分功能未知，感染与致病机制不清，疫苗创制的理论认知有限，其流行已逾百年，但仍未有商品化的疫苗问世。
目前对于非洲猪瘟疫苗的制备主要通过三种方式：一是直接对原始非洲猪瘟病毒进行灭活得到灭活疫苗；二是筛选出有效诱导免疫应答的病毒蛋白，进而制备亚单位疫苗和活载体疫苗；三是采用基因缺失手段，敲除毒力基因后获得重组病毒疫苗。其中第一种方法是制备病毒疫苗最常用、最直接的方法，但是，由于非洲猪瘟病毒编码免疫抑制、免疫耐受和抗体依赖增强作用(ADE)等蛋白，灭活疫苗免疫猪体内无法提供有效的免疫保护作用；第二种方法的困难在于非洲猪瘟病毒结构复杂，目前诱导免疫应答的蛋白还未清楚，获得的抗原还不足以诱导坚强的免疫保护作用。而目前最有望突破的疫苗就是基因敲除疫苗，通过对免疫抑制、耐受、ADE等毒力相关基因的敲除，获得既具有免疫原性，又能降低致病性，免疫猪获得免疫保护作用的疫苗候选毒株。例如，中国专利CN110551695A提供了一种非洲猪瘟病毒四基因缺失弱毒株，该弱毒株是非洲猪瘟病毒SY18分离株的四基因缺失弱毒株，其缺失以下基因的功能蛋白：CD2v基因编码产物和三个多基因家族基因(MGF360-12L、MGF360-13L、MGF360-14L)编码产物，接种仔猪免疫28天后，以ASFV原始毒进行攻毒试验，结果免疫猪群均获得完全保护；又如中国专利CN110093324B公开了一种基因Ⅱ型的非洲猪瘟病毒MGF360-505R缺失和CD2V与MGF360-505R联合缺失的基因缺失病毒，这两种毒株均能提供对非洲猪瘟中国流行强毒株的100％免疫保护，这两株基因缺失病毒免疫后 21天能够提供充分的强毒攻击保护。
综上，对于猪瘟病毒毒力基因的敲除，往往需要考虑到对猪的免疫应答和保护性，又要兼顾致病性和安全性能。但是现有技术中猪瘟病毒毒力基因敲除疫苗还是存在如下问题：①不同毒株缺失同一基因产生的效果不同，缺失不充分还会有毒力和致病性残留，以及多基因缺失造成的病毒滴度低，也会使致弱毒株降低免疫原性或保护作用等风险；②敲除的毒力基因是否减少带毒和排毒是生物安全的一个重要指标，最好能够获得免疫清除的疫苗候选毒株，减少散毒和排毒的风险；③非洲猪瘟的全基因组测序工作虽然已经完成，但是组成非洲猪瘟病毒的调控基因和结构基因高达160多个，虽然对所有基因的功能进行研究是一项巨大的工程，但全面研究每一个调控基因和结构基因的功能对其致病机理和疫苗研制至关重要。
因此，在非洲猪瘟减毒疫苗的制备过程中，鉴定基因功能及其对致病性和免疫应答的影响，为敲除靶标基因的选择和基因敲除的构建提供依据，新靶标基因的筛选至关重要，决定着疫苗候选株的安全性和免疫有效性。本发明发现DP71L具有较强的免疫抑制作用，是新发现的新靶标基因；在非洲猪瘟原始毒株中联合缺失DP71L、DP96R和MGF360-18R基因，获得了一株肌肉注射高剂量(每头猪免疫104HAD50)猪仍不发病的基因缺失毒株，与免疫猪同居的猪的核酸和抗体均是阴性，显示该毒株肌肉注射不能够致猪发病死亡、无水平传播现象，表明它是安全性良好的减毒非洲猪瘟病毒株。本发明的非洲猪瘟病毒弱毒株能对ASFV亲本毒株的攻击提供完全的免疫保护作用，且安全性高，适于作为预防非洲猪瘟的疫苗候选株。
发明内容
针对上述技术问题，本发明提供一种构建非洲猪瘟减毒株的策略，所述策略是通过以下技术方案实现的：
第一方面，本发明提供了一种通过在基因Ⅱ型非洲猪瘟病毒中联合丧失MGF360-18R基因、DP71L基因和DP96R基因编码蛋白的功能制备减毒非洲猪瘟病毒株的应用。
优选地，所述基因Ⅱ型非洲猪瘟病毒包括ASFV CN/GS/2018分离株、 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFVGermany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019中的任一种。
优选地，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示。
优选地，所述MGF360-18R基因编码蛋白的氨基酸序列如SEQ ID NO.2所示，所述DP71L 基因编码蛋白的氨基酸序列如SEQ ID NO.4所示，所述DP96R基因编码蛋白的氨基酸序列如 SEQ ID NO.6所示。
优选地，所述MGF360-18R基因的核苷酸序列如SEQ ID NO.1所示，所述DP71L基因的核苷酸序列如SEQ ID NO.3所示，所述DP96R基因的核苷酸序列如SEQ ID NO.5所示。
第二方面，本发明提供了一种通过在基因Ⅱ型非洲猪瘟病毒中联合丧失MGF360-18R基因、DP71L基因和DP96R基因编码蛋白的功能制备非洲猪瘟疫苗的应用。
优选地，所述基因Ⅱ型非洲猪瘟病毒包括ASFV CN/GS/2018分离株、 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFVGermany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019中的任一种。
优选地，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示。
优选地，所述MGF360-18R基因编码蛋白的氨基酸序列如SEQ ID NO.2所示，所述DP71L 基因编码蛋白的氨基酸序列如SEQ ID NO.4所示，所述DP96R基因编码蛋白的氨基酸序列如 SEQ ID NO.6所示。
优选地，所述MGF360-18R基因的核苷酸序列如SEQ ID NO.1所示，所述DP71L基因的核苷酸序列如SEQ ID NO.3所示，所述DP96R基因的核苷酸序列如SEQ ID NO.5所示。
第三方面，本发明提供了一种通过在基因Ⅱ型非洲猪瘟病毒中联合缺失基因片段制备基因缺失减毒非洲猪瘟病毒株的应用，所述基因片段包括MGF360-18R基因的全部或部分核苷酸序列、DP71L基因的全部或部分核苷酸序列和DP96R基因的全部或部分核苷酸序列。
优选地，所述基因Ⅱ型非洲猪瘟病毒包括ASFV CN/GS/2018分离株、 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFVGermany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019中的任一种。
优选地，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示。
优选地，所述MGF360-18R基因编码蛋白的氨基酸序列如SEQ ID NO.2所示，所述DP71L 基因编码蛋白的氨基酸序列如SEQ ID NO.4所示，所述DP96R基因编码蛋白的氨基酸序列如 SEQ ID NO.6所示。
优选地，所述MGF360-18R基因的核苷酸序列如SEQ ID NO.1所示，所述DP71L基因的核苷酸序列如SEQ ID NO.3所示，所述DP96R基因的核苷酸序列如SEQ ID NO.5所示。
优选地，所述缺失的基因片段为ASFV CN/GS/2018分离株全长序列的第184062-184456 位。
第四方面，本发明提供了一种通过在基因Ⅱ型非洲猪瘟病毒中联合缺失基因片段制备非洲猪瘟疫苗的应用，所述基因片段包括MGF360-18R基因的全部或部分核苷酸序列、DP71L 基因的全部或部分核苷酸序列和DP96R基因的全部或部分核苷酸序列。
优选地，所述基因Ⅱ型非洲猪瘟病毒包括ASFV CN/GS/2018分离株、 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFVGermany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019中的任一种。
优选地，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示。
优选地，所述MGF360-18R基因编码蛋白的氨基酸序列如SEQ ID NO.2所示，所述DP71L 基因编码蛋白的氨基酸序列如SEQ ID NO.4所示，所述DP96R基因编码蛋白的氨基酸序列如 SEQ ID NO.6所示。
优选地，所述MGF360-18R基因的核苷酸序列如SEQ ID NO.1所示，所述DP71L基因的核苷酸序列如SEQ ID NO.3所示，所述DP96R基因的核苷酸序列如SEQ ID NO.5所示。
优选地，所述缺失的基因片段为ASFV CN/GS/2018分离株全长序列的第184062-184456 位。
第五方面，本发明提供了一种基因缺失减毒非洲猪瘟病毒株，所述基因缺失减毒非洲猪瘟病毒株为基因片段缺失的基因Ⅱ型非洲猪病毒，所述基因片段包括MGF 360-18R基因的全部或部分核苷酸序列、DP71L基因的全部或部分核苷酸序列和DP96R基因的全部或部分核苷酸序列。
优选地，所述基因Ⅱ型非洲猪瘟病毒包括ASFV CN/GS/2018分离株、 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFVGermany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019中的任一种。
优选地，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示。
优选地，所述MGF360-18R基因编码蛋白的氨基酸序列如SEQ ID NO.2所示，所述DP71L 基因编码蛋白的氨基酸序列如SEQ ID NO.4所示，所述DP96R基因编码蛋白的氨基酸序列如 SEQ ID NO.6所示。
优选地，所述MGF360-18R基因的核苷酸序列如SEQ ID NO.1所示，所述DP71L基因的核苷酸序列如SEQ ID NO.3所示，所述DP96R基因的核苷酸序列如SEQ ID NO.5所示。
优选地，所述缺失的基因片段为ASFV CN/GS/2018分离株全长序列的第184062-184456 位。
第六方面，本发明提供了一种非洲猪瘟减毒疫苗，所述非洲猪瘟减毒疫苗包括上述第五方面所述的基因缺失减毒非洲猪瘟病毒株。
第七方面，本发明提供了一种制备上述第五方面所述基因缺失减毒非洲猪瘟病毒株的方法，所述的方法是通过基因工程手段将原始基因Ⅱ型非洲猪瘟病毒中MGF360-18R基因的全部或部分核苷酸序列、DP71L基因的全部或部分核苷酸序列和DP96R基因的全部或部分核苷酸序列缺失。
优选地，所述方法为同源重组技术。
第八方面，本发明提供了一种制备上述第五方面所述基因缺失减毒非洲猪瘟病毒株的方法，所述方法包括如下步骤：
(1)pX330载体优化；通过ClonExpress II一步法克隆法去除pX330载体中Cas9酶两端的核定位信号NLS，命名为pX330ΔN；
(2)设计靶向寡核苷酸181/UK-gRNA-L和181/UK-gRNA-R，将寡核苷酸配对插入pX330-ΔN载体中，制备阳性克隆质粒pX330ΔN-181/UKL和pX330ΔN-181/UKR；其中，所述181/UK-gRNA-L的核苷酸序列如SEQ ID NO.8所示，181/UK-gRNA-R的核苷酸序列如SEQ IDNO.9所示；
(3)设计MGF360-18R基因、DP71L基因和DP96R基因组合片段的上下游序列作为同源重组臂，并同时克隆入pUC19载体中，获得重组转移载体p181/UKLR；
(4)在重组转移载体p181/UKLR左、右臂基因序列中间插入eGFP筛选表达盒基因片段p72-eGFP-SV40poly，获得同源重组转移载体p181/UKLR-eGFP；
(5)将同源重组转移载体p181/UKLR-eGFP、pX330ΔN-181/UKL、pX330ΔN-181/UKR和
Figure BDA0002940903910000061
DNA转染试剂充分混匀，共转染至取自2-4月龄健康SPF巴马小型猪的BMDM细胞，直接感染ASFV原始毒株；
(6)病毒株筛选：利用181/UK-check-F/R引物对重组病毒株筛选，获得基因片段缺失的减毒非洲猪瘟病毒株Δ181/UK，所述181/UK-check-F/R引物对为的核苷酸序列如SEQID NO.16-17所示。
本发明的有益效果是：本发明通过联合丧失包括MGF360-18R基因、DP71L基因和DP96R 基因编码蛋白的功能，获得了一种减毒非洲猪瘟病毒株；所述的减毒非洲猪瘟病毒株毒力显著致弱，高剂量不产生发病和致死现象，用此减毒非洲猪瘟毒株免疫后，猪对亲本毒株ASFV CN/GS/2018分离株具有100％保护作用。且与免疫猪同居的猪不存在感染现象，具有良好的安全性能。上述策略为未来成功制备非洲猪瘟疫苗提供了理论依据和实践参考，研究人员可以通过同时丧失MGF360-18R基因、DP71L基因和DP96R基因及已公开的一种或几种基因 (例如CD2v基因、MGF360-12L基因、MGF360-13L基因、MGF360-14L基因、MGF360-505R 基因等)编码的蛋白功能，最终制备出安全有效的非洲猪瘟疫苗。
附图说明
图1基因敲除策略示意图；
图2重组毒纯化过程中初始代次(F0)和高代次(F6)的细胞在显微镜下观察的结果，比例尺＝100μm；
图3基因缺失病毒株Δ181/UK纯净度的PCR检测结果，p72为内参基因，NC是以超纯水为模板的结果；
图4基因缺失病毒株Δ181/UK免疫实验的致死结果图，其中，左边为免疫部分，右边为攻毒部分，每条曲线代表一头动物，横坐标分别为免疫后(dpi)天数或攻毒后(dpc)天数，纵坐标为存活率；免疫期间以野生毒株ASFV CN/GS/2018为对照评价Δ181/UK的致病性，攻毒期间以未经过免疫的猪为对照评价Δ181/UK的免疫保护效果；
图5基因缺失病毒株Δ181/UK免疫和攻毒后动物的体温变化图，其中，左边为免疫部分，右边为攻毒部分，每条曲线代表一头动物，横坐标分别为免疫后(dpi)或攻毒后(dpc)天数，纵坐标为体温；亲本ASFV CN/GS/2018分离株作为免疫阶段的对照，未经免疫的猪作为攻毒阶段的对照；
图6基因缺失病毒株Δ181/UK免疫和攻毒后动物的血液带毒结果图，其中，左边为免疫部分，右边为攻毒部分，每条曲线代表一头动物，横坐标分别为免疫后(dpi)或攻毒后(dpc)天数，纵坐标为病毒拷贝数。
具体实施方式
为使本发明实现的技术手段、创作特征、达成目的与功效易于明白了解，下面结合具体实施方式，进一步阐述本发明。但本发明的保护范围并不局限于以下实施例所述。
以下实施例中所述的实验获得生物安全许可和非洲猪瘟实验室活动许可：
中国农业科学院兰州兽医研究所根据生物安全3级实验室(BSL-3)和非洲猪瘟相关生物安全的相关要求，经兰州兽医研究所生物安全委员会、实验动物伦理委员会、中国农业科学院生物安全委员会、兰州兽医研究所实验动物伦理委员会、兰州兽医研究所生物安全委员会逐级上报，获得农业部关于开展高致病性ASFV病原及动物研究许可，并已在农业农村部备案，符合国家生物安全等级的要求。
以下实施例中所述的实验细胞、病毒以及质粒来源：
原代猪肺泡巨噬细胞(PAM)及原代骨髓巨噬细胞(BMDM)取自2-4月龄健康SPF巴马小型猪，无菌采集细胞后，用红细胞裂解液(购自Biosharp公司)，去除红细胞，低速离心后，弃上清，将细胞沉淀重悬于含有10％FBS(购自PAN公司)的RPMI 1640完全培养基 (购自Gibco公司)中，置于37℃、5％CO2培养箱中培养。BMDM细胞培养需在RPMI 16 40完全培养基中额外添加10ng/mL终浓度的重组猪GM-CSF(购自R&D Systems公司)，置于37℃、5％CO2培养箱中诱导，每2-3天洗涤一次，将未贴壁细胞离心后重新加入新的细胞皿中，换液继续诱导，3-7天后冻存或使用。利用PAM细胞扩增ASFV，并进行病毒含量的滴定，BMDM细胞用于质粒转染和病毒重组实验。
ASFV CN/GS/2018分离株来自国家非洲猪瘟区域实验室(兰州)，属于基因Ⅱ型，公众可通过农业部兽医局批示的委托函获得；病毒效价为5×107TCID50/mL，为PAM细胞扩繁后的第4代种毒，分装保存于-80℃备用。
pX330载体、peGFP-N1载体、pUC19载体均购买于兰州瑞博莱生物科技有限公司；无内毒素的质粒提取试剂盒，购于OMEGA公司。
实验中的操作如果没有特别说明均是本领域知晓的操作方法。
定义
术语“蛋白功能丧失”是指通过对编码蛋白的基因进行敲除、突变或在编码蛋白的基因片段中插入部分基因，使得基因编码蛋白发生移码突变，导致基因编码蛋白的功能丧失。
术语“基因突变”是指基因在结构上发生碱基对组成或排列顺序的改变，就是在一个位点上，突然出现了一个新基因，代替了原有基因，这个基因叫做突变基因，基因突变导致后代的表型中也就突然地出现从未有的新性状。
术语“基因缺失”是指缺失染色体上某一区段及其带有的基因一起丢失，从而引起变异的现象。基因缺失的方法一般是指基因敲除，是用含有一定已知序列的DNA片段与受体细胞基因组中序列相同或相近的基因发生同源重组，整合至受体细胞基因组中并得到表达的一种外源DNA导入技术。基因敲除的方法一般包括：同源重组技术、随机插入突变技术以及RN A干扰技术；其中，同源重组技术又叫基因打靶，是指外源DNA与受体细胞染色体DNA上的同源序列之间发生重组，并整合到预定的位置上，从而改变某些遗传特性的方法，重组的目的是为了敲除某个基因；随机插入突变技术是指利用某些能随机插入基因序列的病毒、细菌或其他基因载体，在目标细胞基因组中进行随机插入突变的细胞库，然后通过相应的标记进行筛选获得相应的基因敲除细胞；RNA干扰技术是指由与生物体内源靶基因mRNA同源的双链RNA特异性引发的靶mRNA降解，导致目的基因表达沉默的一种反向遗传学技术。
在本发明中，以基因Ⅱ型非洲猪瘟病毒株ASFV CN/GS/2018分离株为例，通过同源重组技术，对ASFV CN/GS/2018分离株中的MGF360-18R基因、DP71L基因和DP96R基因的全部或部分核苷酸序列进行敲除，导致MGF360-18R基因、DP71L基因和DP96R基因编码蛋白的功能丧失，进而构建了MGF360-18R基因、DP71L基因和DP96R基因编码蛋白功能丧失的减毒非洲猪瘟病毒，并用于非洲猪瘟疫苗的生产。所述的MGF360-18R基因、DP71L 基因和DP96R基因为相邻基因，之间还包括间隔序列。由于MGF360-18R基因位于DP71L 基因的一端，并与DP71L基因部分重叠(MGF360-18R基因CDS的部分序列及启动子序列)，DP96R基因与DP71L基因的另一端相邻，中间含有部分无基因功能的连接序列，因此可以通过分别缺失MGF360-18R基因、DP71L基因和DP96R基因的全部或部分核苷酸序列导致其编码蛋白功能的丧失；为了简化操作步骤，也可以通过一次操作缺失MGF360-18R基因的部分序列(CDS的部分序列及启动子序列)、DP71L基因全部核苷酸序列、DP96R基因的部分序列及DP71L基因和DP96R基因之间的无基因功能序列的连接序列，使MGF360-18R基因、 DP71L基因和DP96R基因编码的蛋白无法正常表达，导致其编码蛋白功能的丧失；根据本领域技术人员的公知常识，除了上述基因编辑手段，还可以采用其它基因编辑手段使MGF360- 18R基因、DP71L基因和DP96R基因编码的蛋白功能同时丧失，例如：移码突变、点突变、缺失或插入核苷酸序列等。
在本发明中通过缺失DP71L基因全部核苷酸序列、并缺失DP96R基因和MGF360-18R基因的部分序列，使MGF360-18R基因、DP71L基因和DP96R基因编码的蛋白无法表达，导致其编码蛋白功能的丧失，获得了减毒非洲猪瘟重组病毒，降低亲本毒株的毒性的基础上，本领域技术人员通过将MGF360-18R基因、DP71L基因和DP96R基因进行全部或部分突变，也能使MGF360-18R基因、DP71L基因和DP96R基因基因编码的蛋白无法表达，导致其编码蛋白功能的丧失，实现减毒非洲猪瘟重组病毒的构建。其中所述的MGF360-18R基因位于 ASFV CN/GS/2018分离株全基因组序列的183372-184085位，所述的DP71L基因位于ASF V CN/GS/2018分离株全基因组序列的184068-184280位，所述的DP96R基因位于ASFV C N/GS/2018分离株全基因组序列的184379-184669位。所述间隔序列不含有任何功能基因序列；本发明中所述缺失的MGF360-18R基因、DP71L基因和DP96R基因序列位于ASFV CN /GS/2018分离株全基因组序列的184062-184456位(如SEQ ID NO.7所示)，其中包括部分间隔序列。
术语“疫苗”是指能够在动物中提供保护性应答的生物制剂，其中所述疫苗已经被递送并且不能造成严重疾病。本发明的疫苗是遗传工程改造的基因缺失减毒病毒疫苗，其中所述缺失的基因为MGF360-18R基因、DP71L基因和DP96R基因的全部或部分核苷酸序列；突变被理解为在亲本CN/GS/2018ASFV毒株中野生型或未修饰的MGF360-18R基因、DP71L基因和DP96R基因的遗传信息中的变化。应当理解的是，由MGF360-18R基因、DP71L基因和DP96R基因突变获得重组突变体也可以作为减毒病毒疫苗。
本发明的减毒非洲猪瘟病毒疫苗，进一步任选地包含一种或多种佐剂，赋形剂，载体和稀释剂。佐剂可以任何合适的佐剂，化学类免疫佐剂如氢氧化铝、弗氏佐剂、矿物油、司盘等；微生物类免疫佐剂如分枝杆菌、脂多糖、胞壁酰二肽、胞肽、脂溶性蜡质D、短小棒状杆菌；植物类免疫佐剂多为从植物或大真菌中提取的多糖类，如茯苓多糖、红花多糖、中草药类等。而生化类免疫佐剂如胸腺肽、转移因子、白细胞介素等。优选的佐剂可以是纳米佐剂生物佐剂，白介素、干扰素等。
本发明公开的疫苗也可被用于制备联合疫苗，如与猪的其他疫苗联合，但重点在减毒活疫苗上，尤其是病毒基因的整合，如双价苗，三价苗等。联合疫苗可以包含不同基因型的多种减毒非猪瘟病毒，如此可以诱导针对多种非猪瘟病毒基因型的交叉保护性免疫应答。
本发明的疫苗的施用可以采用方便的途径，例如肌内注射，鼻内，口服，皮下，透皮和阴道等途径。本发明的减毒疫苗优选采用肌肉注射。疫苗可以在初免-加强方案后施用。例如，在第一次接种后，受试者可以在一段时间(例如约7，14，21或28天)之后接受第二次加强施用。通常，加强施用的剂量相同或低于初免施用的剂量。此外，也可以进行第三次加强免疫，例如免疫后2-3个月、6个月或一年。
在本发明中虽然以基因Ⅱ型非洲猪瘟病毒株ASFV CN/GS/2018分离株为例，构建了 MGF360-18R基因、DP71L基因和DP96R基因编码蛋白功能丧失的非洲猪瘟减毒株，但是本发明并不局限于ASFV CN/GS/2018分离株。本领域技术人员公知：在不同的ASFV基因组中删除相关的基因后，观察到的表型差异与基因组成相关；并且现有流行的基因Ⅱ型非洲猪瘟病毒(包括China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFVBelgium 2018/1、ASFV Germany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFVWuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019)与本申请所述的基因Ⅱ型非洲猪瘟病毒ASFV CN/GS/2018分离株的基因组成完全相同。因此，在基因Ⅱ型非洲猪瘟病毒的基因组成相同的基础上，本领域技术人员可以合理推断出除本发明所述的ASFV CN/GS/2018分离株外，以其他Ⅱ型非洲猪瘟病毒(包括China/2018/AnhuiXCGQ、 Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、ASFV/POL/2015/Podlaskie、 ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、ASFV/Pol16_20538_o9、 ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、ASFV/Pol17_03029_C201、 ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFV Germany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、 ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019)为出发毒株同样能够成功构建获得相关减毒非洲猪瘟病毒株及疫苗。因此，本发明并不局限于ASFV CN/GS/2018分离株，其他与ASFV CN/GS/2018分离株基因组成相同的基因Ⅱ型非洲猪瘟病毒均在本发明的保护范围之内，其中所述与ASFV CN/GS/2018分离株基因组成相同的基因Ⅱ型非洲猪瘟病毒包括但不局限于 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFV Germany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019。
实施例1重组病毒Δ181/UK的构建及纯化鉴定
1.CRISPR/Cas9载体构建
(1)pX330载体优化：由于非洲猪瘟病毒主要在细胞质病毒工厂内复制，因此在构建p CRISPR/Cas9载体时，首先将pX330进行优化；通过ClonExpressⅡ一步法克隆法去除其Ca s9酶两端的核定位信号(NLS)，命名为pX330ΔN。
(2)MGF360-18R基因、DP71L基因和DP96R基因为相邻基因，设计针对DP71L和DP96R基因的靶向gRNAs，其寡核苷酸名称和序列分别为：181/UK-gRNA-L：GCTCCTCCACGCTCGCGATCCGG(SEQ ID NO.8所示)；181/UK-gRNA-R：TTACAGGAAGAATAATGG GGAGG(SEQ IDNO.9所示)。
(3)参照文献(Ran FA,Hsu PD,Wright J,Agarwala V,Scott DA,Zhang F.Genomeengineering using the CRISPR-Cas9 system.Nat Protoc.2013；8(11):2281-2308)推荐的克隆方法，将寡核苷酸181/UK-gRNA-LF和181/UK-gRNA-RF分别插入到pX330-ΔN载体中，提取质粒DNA，经测序正确后，分别将阳性克隆质粒命名为：pX330ΔN-181/UKL和pX330 ΔN-181/UKR。用无内毒素的质粒提取试剂盒提取DNA，测定浓度，-20℃保存备用。
2.筛选表达盒构建
为了便于筛选，共构建一套筛选标记基因的表达盒，即增强型绿色荧光蛋白(Enhance Green fluorescent protein，eGFP)基因筛选表达盒构建：参照文献(O'DonnellV,Holinka L G,Krug PW,Gladue DP,Carlson J,Sanford B,Alfano M,Kramer E,Lu Z,Arzt J,Reese B,Carrillo C,Risatti GR,BorcaMV.African Swine Fever VirusGeorgia 2007with a Delet ion of Virulence-Associated Gene 9GL(B119L),whenAdministered at Low Doses,Leads t o Virus Attenuation in Swine and Induces anEffective Protection against Homologous Chall enge.JVirol.2015；89(16):8556-66)，通过PCR扩增p72启动子(从p72基因上游的-196nt到 +17之前序列)，备用；扩增引物为：正向引5’-TTATAAAACATATGTTCATAAAAAGGGTCG CCGGAGGAAAAGTC-3’(SEQ ID NO.10所示)和反向引物5’-CTCCTCGCCCTTGCTCAC CATATATAATGTTATAAAAATAATT-3’(SEQ ID NO.11所示)；以peGFP-N1载体为模板，扩增eGFP基因，备用，扩增引物为：正向引物5’-ATGGTGAGCAAGGGCGAGGAG-3’(SEQ ID NO.12所示)和反向引物5’-ACCACAACTAGAATGCAGTG-3’(SEQ ID NO.13所示)；参照文献(Borca MV,Holinka LG,Berggren KA,GladueDP.CRISPR-Cas9,a tool to effic iently increase the development of recombinantAfrican swine fever viruses.Sci Rep.2018；8 (1):3154.)，将上述步骤扩增获得的p72启动子和eGFP两个基因通过融合PCR的方法连接，获得eGFP筛选表达盒基因片段，命名为p72-eGFP-SV40polyA，该表达盒序列含SV40polyA 终止序列。
3.同源重组转移载体的构建
利用pUC19载体作为骨架载体，构建MGF360-18R基因、DP71L基因和DP96R基因敲除用同源重组转移载体，所述MGF360-18R基因的核苷酸序如SEQ ID NO.1所示，所述的D P71L基因的核苷酸序如SEQ ID NO.3所示，所述DP96R基因的核苷酸序如SEQ ID NO.5 所示，所述的MGF360-18R基因位于ASFV CN/GS/2018分离株全基因组序列的183372-184 085位，所述DP71L基因位于ASFV CN/GS/2018分离株全基因组序列的184068-184280位，所述DP96R基因位于ASFV CN/GS/2018分离株全基因组序列的184379-184669位，所述缺失的基因序列位于ASFV CN/GS/2018分离株全基因组序列的184062-184456(如SEQ ID N O.7所示)，构建策略见图1。
具体步骤为：由于MGF360-18R基因、DP71L基因和DP96R基因为相邻基因，所以设计MGF360-18R基因、DP71L基因和DP96R基因组合片段(位于ASFV CN/GS/2018分离株全基因组序列的184062-184456)的上下游序列约1.0kb作为同源重组左臂(Left arm，序列如SEQ IDNO.14所示)和右臂(Right arm，序列如SEQ ID NO.15所示)，并分别克隆入 pUC19载体中，获得三基因的重组转移载体；在重组转移载体左、右臂基因序列中间插入e GFP筛选表达盒基因片段p72-eGFP-SV40poly；经测序正确后，将该同源重组转移载体命名为p181/UKLR-eGFP；用无内毒素的质粒提取试剂盒提取DNA，测定浓度，-20℃保存备用。
4.细胞转染和重组病毒筛选
选取状态良好的骨髓巨噬细胞(BMDM)，接种ASFV CN/GS/2018毒株(MOI＝1.0)，约3-4h更换为正常半量培养基，将同源重组转移载体p181/UKLR-eGFP(2μg)、pX330ΔN- 181/UKL(1μg)、pX330ΔN-181/UKR(1μg)和JET PEI-Macrophage DNA转染试剂(12μL) 充分混匀，共转染至BMDM细胞，4h后更换为正常量培养基，培养箱培养，24h后即可观察。结果如图2中A和B所示，在荧光显微镜下观察发现，低代次(F0)时，BMDM中只能观察到少量的eGFP信号，随后消化收集细胞，少量培养基重悬后滴加至新的培养皿中，待细胞沉降后，在荧光显微镜下挑取转染孔中单个荧光细胞至96孔板，-80℃反复冻融2次后，接至96孔板单层BMDM细胞中，24h后观察到荧光细胞，证明重组毒基本构建成功。接毒72h后，选择阳性孔按如上步骤挑取单细胞，将剩余的当代次阳性孔重组毒-80℃反复冻融2次后，一分为二接种至预先铺好的96孔板BMDM中，一孔提取病毒基因组DNA，进行检测，另一孔冻存待扩增。具体检测方法如下：利用p72-eGFP定点整合检测引物对进行PC R鉴定，检测p72-eGFP-SV40pA元件是否整合到了目的基因位点。纯净性检测的181/UK-ch eck-F/R引物对为：AAACTACGCTCGCAGCGCAAAAAG(SEQ ID NO.16所示)和ATCC ACGACAACTGATTTCTCAGA(SEQ IDNO.17所示)，同时选取病毒基因组中的p72为内参基因，确保所检测病毒基因组的质量合格，针对p72检测的引物分别为p72-F：CCGGGGT ATTCGCAGTAGTA(SEQ ID NO.18所示)和p72-R：GCAGCTTCAAACGTTTCCTC(SE Q ID NO.19所示)。
经多次单细胞传代后，在高代次(F6)时荧光显微镜下观察结果如图2中C和D所示，部分孔中荧光细胞数量比例可达100％，为全阳性孔，这说明重组毒构建基本成功。纯净性检测结果如图3所示，其中以ASFV CN/GS/2018分离株为阳性对照，以水为阴性对照，Δ181/UK-#1-2代表Δ181/UK重组病毒，结果表明，这些重组病毒的基因组中均已经检测不到MGF360-18R、DP71L和DP96R基因组合片段，表明MGF360-18R基因、DP71L基因和DP96R 基因组合片段敲除成功；即，本实施例通过缺失了DP71L基因全部核苷酸序列、DP96R基因的绝大部分核苷酸序列，并缺失了与DP71L基因的重叠的MGF360-18R基因的启动子序列，使MGF360-18R基因、DP71L基因和DP96R基因基因编码的蛋白无法表达，导致其编码蛋白功能的丧失，成功构建了MGF360-18R、DP71L和DP96R基因缺失的减毒重组病毒，并且已纯化，将减毒重组病毒命名为Δ181/UK。
实施例2病毒滴度的测定
非洲猪瘟病毒的滴度采用半数血细胞吸附量(50％haemadsorption,HAD50)表示，HAD 50实验具体操作见文献(Borca MV,Ramirez-Medina E,Silva E,Vuono E,Rai A,Pruitt S, Holinka LG,Velazquez-Salinas L,Zhu J,GladueDP.Development of ahighly effective Afri can swine fever virus vaccine by deletion of the I177Lgene results in sterile immunity aga inst the current epidemic Eurasiastrain.JVirol.2020.pii:JVI.02017-19)，同时对其进行适当调整：在96孔板中按照约1x105/孔细胞接种原代PAM细胞，待检重组毒连续10倍梯度稀释，共7个稀释度，每个稀释度下8孔，按照100μL/孔将稀释毒加至96孔板PAM中，随即加入红细胞，共三次重复。病毒感染可根据红细胞在感染细胞周围聚集形成的花环进行判定，连续观察6天，统计阳性孔数，计算半数血细胞吸附量(HAD50)。滴度测定合格，进行致病性评价。
实施例3基因缺失毒株Δ181/UK的毒力评价
为了检测基因缺失毒株Δ181/UK的毒力，本实验用104HAD50剂量经肌肉注射猪对其毒力进行评价。
本实验用13头非洲猪瘟抗原抗体阴性的健康长白仔猪，共分为3组，其中攻亲本毒株A SFV CN/GS/2018分离株和基因缺失毒株Δ181/UK组各5头，另外3头实验猪为Δ181/UK接毒组的同居对照，攻毒后每天测量体温变化情况，采集外周血和唾液，参照文献(KingDP, Reid SM,Hutchings GH,Grierson SS,Wilkinson PJ,Dixon LK,Bastos AD,DrewTW.20 03.Development of a TaqMan PCR assay with internal amplificationcontrol for the detecti on of African swine fever virus.J Virol Methods 107:53-61)，通过荧光定量PCR方法测定血液中的ASFV病毒含量，观察至19天终止。统计实验组和对照组动物的致死率和体温变化。存活率实验结果如图4(免疫部分)所示，和体温变化实验结果如图5(免疫部分)所示，亲本毒株经过肌肉注射后，实验猪出现ASFV典型症状，持续高温发热，第5天开始出现死亡，到第7天全部死亡；而基因缺失毒株Δ181/UK经过肌肉注射后，虽然有2头实验猪(总共5 头)出现瞬时的高温反应，但最终都下降到正常体温，存活率为100％；并且Δ181/UK同居猪体温正常，并未出现死亡，存活率为100％。
血液中的ASFV病毒含量检测结果如图6(免疫部分)所示，注射亲本毒株ASFV CN/GS/2018分离株的实验猪在接毒后血液中ASFV病毒量急速上升(鉴于第5天已经有实验猪死亡，且其余实验猪出现了明显的非洲猪瘟临床症状，所以接毒第5天以后没有继续采血检测带毒情况)，而注射基因缺失毒株Δ181/UK的实验猪血液带毒量明显减少，并且血液中病毒增殖速度缓慢。此外，Δ181/UK的3头同居实验猪的血液中未检测到明显的带毒情况。
上述实验结果表明，使亲本ASFV CN/GS/2018分离株中MGF360-18R基因、DP71L基因和DP96R基因编码蛋白的功能丧失后，获得的基因缺失毒株Δ181/UK的毒力相较于亲本ASFV CN/GS/2018分离株的毒力显著致弱，并且实验动物之间不存在水平传播。
实施例4缺失病毒Δ181/UK的免疫保护效果评价
为了检测基因缺失毒株Δ181/UK的免疫保护效果，本实验用102HAD50剂量的亲本ASF V CN/GS/2018分离株对实施例3中经过基因重组毒株Δ181/UK免疫的5头实验猪和3头未免疫过的实验猪(对照组)进行攻毒。
攻毒后每天测量体温变化情况，采集外周血，观察至13天终止，野生ASFV攻毒后免疫组未出现发热的典型病症，而对照组中实验猪的体温明显升高，体温监测结果如图5(攻毒部分)所示，其中横坐标为攻毒时间，纵坐标为体温，Δ181/UK为重组毒株免疫注射组猪；对照组猪出现ASFV典型症状，持续高温发热，第7天开始出现死亡，到第9天全部死亡；而基因缺失毒株Δ181/UK免疫注射组猪的存活率为100％。这些结果初步说明，在亲本ASF VCN/GS/2018分离株攻毒后，相对于对照组，基因缺失毒株Δ181/UK免疫组中实验猪的体温显著降低，即基因缺失毒株Δ181/UK对亲本ASFV CN/GS/2018分离株具有完全的免疫保护效果。
本实验参照文献(King DP,Reid SM,Hutchings GH,Grierson SS,Wilkinson PJ,Dixon LK, Bastos AD,Drew TW.2003.Development of a TaqMan PCR assay withinternal amplification control for the detection of African swine fevervirus.J Virol Methods 107:53-61)，通过荧光定量 PCR方法测定血液中的ASFV病毒含量，对免疫后攻毒阶段的实验动物血液带毒情况进行了检测。从图6(攻毒部分)可知，在免疫后攻毒阶段，未经免疫的对照组猪血液中病毒含量急速升高，而经过Δ181/UK减毒株免疫的猪血液中病毒含量非常低，并且随着时间的推移，有逐步降低的趋势。这些结果进一步说明，基因缺失毒株Δ181/UK对亲本ASFV CN/GS/2018 分离株有完全的免疫保护效果。
需要说明的是，在本发明中虽然以基因Ⅱ型非洲猪瘟病毒株ASFV CN/GS/2018分离株为例，构建了MGF360-18R基因、DP71L基因和DP96R基因编码蛋白功能丧失的非洲猪瘟减毒株；但是本发明并不局限于ASFV CN/GS/2018分离株，其他与ASFV CN/GS/2018分离株基因组成相同的基因Ⅱ型非洲猪瘟病毒均在本发明的保护范围之内；所述与ASFV CN/GS/2018分离株基因组成相同的基因Ⅱ型非洲猪瘟病毒包括但不局限于 China/2018/AnhuiXCGQ、Pig/CN/HLJ/2018、Georgia 2007、ASFV-SY18、 ASFV/POL/2015/Podlaskie、ASFV/Pol17_05838_C220、ASFV/Pol16_29413-o23、 ASFV/Pol16_20538_o9、ASFV/Pol17_04461_C210、ASFV/Pol16_20186_o7、 ASFV/Pol17_03029_C201、ASFV/Pol16_20540_o10、ASFV CzechRepublic 2017/1、ASFV Moldova 2017/1、ASFV Belgium 2018/1、ASFVGermany 2020/1、ASFV Arm/07/CBM/c2、ASFV Ken.rie1、ASFV Wuhan 2019-2、ASFV_HU_2018、ASFV/pig/China/CAS19-01/2019。
序列表
<110> 中国农业科学院兰州兽医研究所
<120> 非洲猪瘟基因缺失弱毒株的构建及其作为疫苗的应用
<160> 20
<170> SIPOSequenceListing 1.0
<210> 1
<211> 714
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 1
atgttagaaa tagtattggc aacgctgcta ggcgacctgc agcggctccg ggttcttacc 60
cctcagcagc gggcagttgc cttctttcga gccaatacta aggagctaga ggacttctta 120
tgctcagatg ggcagtctga ggaggtactg tctggccccc ttcttaaccg tctactagaa 180
ccctcaggcc ctcttgatat tttaaccgga tatcacctat ttcgtcagaa tcccaaggca 240
ggtcagttgc gcggccttga ggtcaagatg cttgaacggt tatacgatgc taatatttac 300
aatatactgt ctcggctgcg gcctgaaaaa gttcgcaaca aggctattga gctatactgg 360
gttttccgag ctatccatat ttgtcatgct cctttagttt tagatattgt acgatatgag 420
gaaccggact ttgctgaact ggcctttatt tgtgctgctt actttggtga acctcaggta 480
atgtatttgc tctacaaata tatgcctctg acccgcgcag ttcttacgga tgccatccgg 540
ataagtcttg agagcaacaa ccaggtaggg atttgctatg cttacttgat gggaggcagc 600
ctcaagggac tagtctccgc cccactgcgt aaacgtctgc gcgccaaact acgctcgcag 660
cgcaaaaaga aggacgttct ttcaccccac gacttcttac tgctgctcca gtag 714
<210> 2
<211> 237
<212> PRT
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 2
Met Leu Glu Ile Val Leu Ala Thr Leu Leu Gly Asp Leu Gln Arg Leu
1 5 10 15
Arg Val Leu Thr Pro Gln Gln Arg Ala Val Ala Phe Phe Arg Ala Asn
20 25 30
Thr Lys Glu Leu Glu Asp Phe Leu Cys Ser Asp Gly Gln Ser Glu Glu
35 40 45
Val Leu Ser Gly Pro Leu Leu Asn Arg Leu Leu Glu Pro Ser Gly Pro
50 55 60
Leu Asp Ile Leu Thr Gly Tyr His Leu Phe Arg Gln Asn Pro Lys Ala
65 70 75 80
Gly Gln Leu Arg Gly Leu Glu Val Lys Met Leu Glu Arg Leu Tyr Asp
85 90 95
Ala Asn Ile Tyr Asn Ile Leu Ser Arg Leu Arg Pro Glu Lys Val Arg
100 105 110
Asn Lys Ala Ile Glu Leu Tyr Trp Val Phe Arg Ala Ile His Ile Cys
115 120 125
His Ala Pro Leu Val Leu Asp Ile Val Arg Tyr Glu Glu Pro Asp Phe
130 135 140
Ala Glu Leu Ala Phe Ile Cys Ala Ala Tyr Phe Gly Glu Pro Gln Val
145 150 155 160
Met Tyr Leu Leu Tyr Lys Tyr Met Pro Leu Thr Arg Ala Val Leu Thr
165 170 175
Asp Ala Ile Arg Ile Ser Leu Glu Ser Asn Asn Gln Val Gly Ile Cys
180 185 190
Tyr Ala Tyr Leu Met Gly Gly Ser Leu Lys Gly Leu Val Ser Ala Pro
195 200 205
Leu Arg Lys Arg Leu Arg Ala Lys Leu Arg Ser Gln Arg Lys Lys Lys
210 215 220
Asp Val Leu Ser Pro His Asp Phe Leu Leu Leu Leu Gln
225 230 235
<210> 3
<211> 213
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 3
ttactgctgc tccagtagct ttttttgccg caggagcacc gcggatagga gctcctccac 60
gctcgcgatc cggcgctgga agcggaaccg atcgaccgcc acctgctccc agggaccctt 120
gcgctcgatg tcgtcggctt cccacacctc gacggctgtg gcaaaatgga catgcttcgc 180
gtcgttcgtc cgttttttgc gccgcctccc cat 213
<210> 4
<211> 70
<212> PRT
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 4
Met Gly Arg Arg Arg Lys Lys Arg Thr Asn Asp Ala Lys His Val His
1 5 10 15
Phe Ala Thr Ala Val Glu Val Trp Glu Ala Asp Asp Ile Glu Arg Lys
20 25 30
Gly Pro Trp Glu Gln Val Ala Val Asp Arg Phe Arg Phe Gln Arg Arg
35 40 45
Ile Ala Ser Val Glu Glu Leu Leu Ser Ala Val Leu Leu Arg Gln Lys
50 55 60
Lys Leu Leu Glu Gln Gln
65 70
<210> 5
<211> 291
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 5
atgtctacac atgattgttc tctaaaagag aaaccggttg atatgaacga tatatctgag 60
aaatcagttg tcgtggataa tgcacccgag aaaccagctg gagcgaatca tatacctgag 120
aagtcggccc gcgaaatgac atcatcagaa tggattgctg aatattggaa aggtataaaa 180
cgtggaaatg acgtgccatg ttgttgtcca agaaaaatga ccagtgcaga caaaaagttt 240
tcagtatttg gtaagggatc cctaatgcgc tccatccaga agaataatta a 291
<210> 6
<211> 96
<212> PRT
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 6
Met Ser Thr His Asp Cys Ser Leu Lys Glu Lys Pro Val Asp Met Asn
1 5 10 15
Asp Ile Ser Glu Lys Ser Val Val Val Asp Asn Ala Pro Glu Lys Pro
20 25 30
Ala Gly Ala Asn His Ile Pro Glu Lys Ser Ala Arg Glu Met Thr Ser
35 40 45
Ser Glu Trp Ile Ala Glu Tyr Trp Lys Gly Ile Lys Arg Gly Asn Asp
50 55 60
Val Pro Cys Cys Cys Pro Arg Lys Met Thr Ser Ala Asp Lys Lys Phe
65 70 75 80
Ser Val Phe Gly Lys Gly Ser Leu Met Arg Ser Ile Gln Lys Asn Asn
85 90 95
<210> 7
<211> 395
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 7
gacttcttac tgctgctcca gtagcttttt ttgccgcagg agcaccgcgg ataggagctc 60
ctccacgctc gcgatccggc gctggaagcg gaaccgatcg accgccacct gctcccaggg 120
acccttgcgc tcgatgtcgt cggcttccca cacctcgacg gctgtggcaa aatggacatg 180
cttcgcgtcg ttcgtccgtt ttttgcgccg cctccccatt attcttcctg taagattagt 240
gtttaatacc tataataaca taattttaag atttaatata ccaaaactta aactattttt 300
gtatagtaac tattagcatg tctacacatg attgttctct aaaagagaaa ccggttgata 360
tgaacgatat atctgagaaa tcagttgtcg tggat 395
<210> 8
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
gctcctccac gctcgcgatc cgg 23
<210> 9
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
ttacaggaag aataatgggg agg 23
<210> 10
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
ttataaaaca tatgttcata aaaagggtcg ccggaggaaa agtc 44
<210> 11
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
ctcctcgccc ttgctcacca tatataatgt tataaaaata att 43
<210> 12
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
atggtgagca agggcgagga g 21
<210> 13
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
accacaacta gaatgcagtg 20
<210> 14
<211> 1008
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 14
acaagaggaa taggggccat tactaacatt ggctccaaca tcctgttgtc tacaaaaaaa 60
aatatttttt ttagcaaaaa aaaatccatg gaaggatatt aatacacata attatttgac 120
atcacattag tgtacttacc aaatagtaat atacaaccat cctaatattc acctttatga 180
aatgatccca acctatacgg taaaatagta taggttttaa taaagaaaaa agatattctg 240
tggtttttat ttttgtatag tgtgtgaata caaaataaaa tcccaaattt taaccttttc 300
tttttttttc tatacaggat gttagaaata gtattggcaa cgctgctagg cgacctgcag 360
cggctccggg ttcttacccc tcagcagcgg gcagttgcct tctttcgagc caatactaag 420
gagctagagg acttcttatg ctcagatggg cagtctgagg aggtactgtc tggccccctt 480
cttaaccgtc tactagaacc ctcaggccct cttgatattt taaccggata tcacctattt 540
cgtcagaatc ccaaggcagg tcagttgcgc ggccttgagg tcaagatgct tgaacggtta 600
tacgatgcta atatttacaa tatactgtct cggctgcggc ctgaaaaagt tcgcaacaag 660
gctattgagc tatactgggt tttccgagct atccatattt gtcatgctcc tttagtttta 720
gatattgtac gatatgagga accggacttt gctgaactgg cctttatttg tgctgcttac 780
tttggtgaac ctcaggtaat gtatttgctc tacaaatata tgcctctgac ccgcgcagtt 840
cttacggatg ccatccggat aagtcttgag agcaacaacc aggtagggat ttgctatgct 900
tacttgatgg gaggcagcct caagggacta gtctccgccc cactgcgtaa acgtctgcgc 960
gccaaactac gctcgcagcg caaaaagaag gacgttcttt caccccac 1008
<210> 15
<211> 948
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 15
aatgcacccg agaaaccagc tggagcgaat catatacctg agaagtcggc ccgcgaaatg 60
acatcatcag aatggattgc tgaatattgg aaaggtataa aacgtggaaa tgacgtgcca 120
tgttgttgtc caagaaaaat gaccagtgca gacaaaaagt tttcagtatt tggtaaggga 180
tccctaatgc gctccatcca gaagaataat taaaaaaaat atttttttta gcaagttttt 240
aaactattta aataaatgtg gtaaaaaaat tcacataata attaaagtga acgtgttaga 300
attaatattt ttttataatc ggatataata tccattaaat caataaatga tagtgttgct 360
accacactaa acaataacaa acagaaacgc acgatacctt tcctcatgat ttataatagc 420
gtgttatcta aagatttttt tgaaaaaaat attaaatttt agttgattat ttttttcagt 480
tacaacattg ctttagaaaa aatacctaat tactacatag caaataaagc gagcgcattg 540
ttacaaacaa catttttttg cgcctggata ctcctatata tgagaactat aatacggtat 600
attaatccta ttaccaacat tgtcaataat agtatgtagg caatgacata ctttaaatac 660
caaatatcca tggttatttc taaaaatctt gaaaaaacgt taaattttag atcggtcacc 720
tacgacagta atactaattt taataattga tgactgaaat cataatataa tgccgtgcga 780
aaaataatta tttttcggtt aaagatacca ttacataaaa aatatgccat ctactctaca 840
agtgcttgct aaaaaggtat tggccttagg ggagcataaa gaaaatgaac atatatctag 900
agaatattat tatcatatat taaagtgttg cggtttatgg tggcatga 948
<210> 16
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
aaactacgct cgcagcgcaa aaag 24
<210> 17
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
atccacgaca actgatttct caga 24
<210> 18
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
ccggggtatt cgcagtagta 20
<210> 19
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
gcagcttcaa acgtttcctc 20
<210> 20
<211> 189393
<212> DNA
<213> 非洲猪瘟病毒(African swine fever virus)
<400> 20
atataccata ttattgctat tgccatcaat gagaatgcca cgtaggcata ggtcatccta 60
tggccggacc aatccatggc tgcacttaaa aatatcaaaa aaagtttaag ttttgggccg 120
gcgttaaaat ttaaaccttt tctggttgat ctttagccat gtatagctgc gatgtttggt 180
gccttatcta catgctattg gcattcctga tattcgcact aaagtgctat gttacaaccg 240
tcttatgcgt gatttttatc caccttattg gccgaagggc cgccttgtat ttcctgttag 300
gtggtttggc cgtattctac tggtggcaag cagctatcaa taaaatttaa tggctctcac 360
ttaagatcct tgctgtaagc gggcgtttac atactttgat caagaaaaaa aattattttt 420
ggaccccccc ccatgtttta tacaaaaatc atataataaa gtggcgacaa tcaacatatt 480
aatcaaccac agcattttat gatgtgttaa tcaacatata ccatattaat caaccacagc 540
attttatgat gcgtcaatca acatattatt acggagagcg tcaatcaata taatattgag 600
aacagcgact tgataccgtg tatggtggtg gcggcggcat gttgtttgta acagcatttt 660
tcatcattcg aagcttacaa aagatatgta taagatagca tattaatgtt attaacagta 720
atatcaataa ggcgtagcta tagatcttca ctttggtaga ccaataatcc atggttgcgc 780
ttaaaaatac caaaaaaaca ttaagttttg gagggtaaga ttggtttttc accattggta 840
aagattatta ttctaaatgt ttaccccata gatgtgaaac aatgattctt catatattaa 900
catatttttt gacttatact tttcttcatc tagtaaggcg ttaatttttt ccggatctgt 960
cgtttttatt gataaaagag aagagtctgg actgtaattt ttaaataata agatatttat 1020
taatatccaa tttattcgtt tggctcgcta tttccatgct ctcttcgaaa gcatcagctc 1080
ctaaatctat acaaaggaat aagttacctt cacaaaaatt cattaccgag gtaatcattg 1140
cccgattaat gtcagccccc aacataaaac aataatatat agttgtataa ttacaatcat 1200
acatacaggc caactgcatc atttcatcaa tgtctatatt tgtcttctct ttgttataaa 1260
tttcatgaag gtcaaagacg ttgttataag caaccccaca tattaaccgc caatctttaa 1320
aatgactata tcgttgataa aaatattgga tggcttcagt aagcttatat agtatcgcca 1380
tactatacca atacctagtt agcatttcgt tgaatgaaat attatccaat gtaaagttaa 1440
ttgataatgt atctagttca ccaaaaattc ttaatttcag ttgagcatta tttaggaaaa 1500
ggggattatc agataataat tcatggcata gaataatatt actgctagtt ttaacatact 1560
gtacattata aaatatttct aaaattttat tttcactcaa agctttcctc gcacctaact 1620
tttggcatag gtcctggtgc actccatatt gacagtaacc aacccaaagc tgatgtctgc 1680
accccattcg gtaaacagct ctattaaacc atgattgttt tcctgtacag ccttcattaa 1740
tgcaacattt aatgttaaac catgtttaaa acttgctgtt tttattaata tttgttcatc 1800
tatacaagta tgataaatcg taattggggc ttcatgccac cacaaaccac aacgctctaa 1860
aatacaataa tcatctttta acacaggctg tgtagctagt acttttttag taagtgcttg 1920
taaagtagat ggcatcttct atctgcaaaa taattatttc cgaaaaaaaa atcaaattaa 1980
aatactaaat tctatttttt ttttaataaa gcctgtaaat tatataataa atctcgccca 2040
ccgtattatt tccggacaca actttttata cctcattata tttttagatc tatagttttt 2100
taacaaggca ttaatttttt ctggatctgt cgtttttaaa gataaaagag agacgtttga 2160
actataataa tctttaaatg ataatatttc tactaatata tcatgattct tttgttttgc 2220
taattctaag ctctcttcga aagcattagc tcctaaatct atacaaaaga acaagttatt 2280
catataaaag ttttttaccg aggtaaccat tgcccgattg atgtcagccc ccaatacaaa 2340
acaatagtaa atggttaaaa aattgctatc tctcatacag gccgatatat catttcatca 2400
atattcatat caaccttttt tatatgatac atttcatgaa gatcagacac gttattaaaa 2460
gaaagcccac atattagccg ccaatcttta aaatgactat atcgttgata aaaatattgg 2520
atggcttcag taagcttaca tagtatcgct atactatacc aatatctagt tagcatttcg 2580
ttgaatgtta tttcattcaa tataaagttg atcgatatct tctctagaaa acaacaaatt 2640
attactttta attcctctat attctggaaa aggggattat tagataacaa tttatggcat 2700
aaaataatat tactactagt tttaatacga tgtattttat aaaatatttg tacaatatcc 2760
atttcattca aaatttttgc gcctaactcc cggcagaaat tccaagtatg ctccgtattg 2820
acagtgacta agctagagtt gatgtctgca ccccattcag taaacaactc tattagatca 2880
tagttgtttt cctgcacagt tttcattaat gcgagattta actctaaacc atctttaaaa 2940
attgctgatt ttatcatcaa ttgattatcc tcattagtag aaagcataat tggagctcca 3000
tgccaccaca aaccacaata tttcaaaata aagtagtgtt ctttagatat gtgctgtgtg 3060
gccagtattt ttttagcaag agcctgcaga gaaattggag tagacatatt tttttttgca 3120
aaatggttta agtttttcaa gaatacagat tggataaatt aggttgttga cttagttaca 3180
ggaggtatta aatattatgt agacataaaa atgagatcct ccaaaaaaat aaacaacaaa 3240
aaaaaatatg tttaatatta aaatgacaat ttctacattg cttattgctc ttattatact 3300
acttattatt attttagtag tgtttttata ctataagaaa caacaaccac cgaaaaaggt 3360
ctgtaaagta gataaagatt gtggtagtgg agagcattgt gttcgtggat catgtagctc 3420
attgagctgc ttagatgccg taaaaatgga caaacgaaat attaagatag attctaagat 3480
ttcctcatgc gaattcactc ccaattttta ccgttttacg gatactgctg ctgatgagca 3540
gcaagaattt ggaaaaacac ggcatcctat aaaaataact ccatctccaa gtgaatccca 3600
tagcccccaa gaggtgtgtg aaaaatattg ttcatgggga accgatgact gtacaggttg 3660
ggaatatgtt ggtgatgaaa aggagggaac atgttatgta tataataatc cacatcaccc 3720
ggttcttaaa tatggtaagg atcacatcat agccttacct agaaatcata aacatgcata 3780
aataaataca ttaggctcat cgtatctttt taaaatccat aaatattcgt ttgatatatg 3840
ctgaaatttt tataaaaaaa aataactatt tcctataaat catctagaaa tagtcctcgt 3900
tttgatcggt ttatatctta taatattgtg catcgatgca caactgcttt ttttggtcct 3960
tctggaacat cattatattt tctttcatta atataccatt cagatgtaaa cgttgaataa 4020
tttttatggc aacaatctac cattgaatta tatttagtaa catctaatac atcgtttgtt 4080
ttatcaggct cagctctata atcttgataa tttttgttat cagcttctaa agctccatca 4140
ttatttttca aagaagtatc cataattatg tttggtaaaa atactttaag ttttaatgtg 4200
atatttaaaa tggttgttat ataaatttac cgcttacagg taatctttat tcagtgtcat 4260
aaactatact tttgatgatt cagtattttg tgaatcagta catttattat cattaatatt 4320
tttaggctgt ttttccaatg ttttattgtt gcaatgagcc tgctcctcct ttgacgagga 4380
agtgtctgtt ggagtcatct gtttaggaag agtatcatcc atatctatta tgaagaaaat 4440
atataaatat tgatatacaa tcaaaaatat ttttgatcac gtctttgtta tctatcgata 4500
ttgttgataa cgtcttgaat aacctacatc atttttttac ataaaaaaat agatataatt 4560
tttattatat ctcaattatt ttaaagataa ttatcaatac agcaaatatc ataagctaac 4620
atatttttcg aataatagtt ttttagtaaa gtattaatct tttcaggatt ggtttctttt 4680
gataataaga taggattcgc tttataaatt tttaaagata atatattcac aatgatagaa 4740
taaccgtata tatctgctaa tgtcttactg tgttcaataa cattagcccc taaatccata 4800
caaaagaaca tattttcaat acaaaagttt tttaccgaga ttaacattgc tcgattagcg 4860
ttggctccca atgcaaaaca gtagtaaatg gtcaaaaaat tattatcgcg catacaggcc 4920
agctccatca ttttattaat actcatatga attttcgttg tgttacatat ttcatgaagg 4980
tcaaacacat tgttgaaaga aagtgcacaa attaatcgcc attcatcaaa atgcctgtat 5040
tcttgacaaa aatattgaat agcttcttta agattatatt ttaccgctat gccataccaa 5100
tatttggtta gcatctcact aaatgagatc tcatttaaca tagaatttgt tgttaaatcc 5160
ttcaactccc aataaatgat catccttaaa tccaccatgt ttacattttg taaaaaaggg 5220
ttattagaaa ataattcatg acacaaaatg acattactac ttgttatttt acactttgtt 5280
tcaaagaaaa atcgtaaaat ttcacttgtc tcaagctctt ctttagctcc caattttcgg 5340
cataggtttc gagtatgctc gttattaata aaaagtaacc cataattaat atttgcaccc 5400
cattcagtaa acaacatgat tagatcatca ttgttttcct taactgccaa taccaatgca 5460
gtattaagcc ttataccctc tttaaagcat aatgtcctta tcattatttg attatcatca 5520
tctatataca ttgagatagg agcttcatgc caccataaac cataacgctc taaaatataa 5580
taatcatctt tagatacgtg ttgcgtggcc aatgcccttt tagcaagtgc ttgtaaagtc 5640
gatggctgca tgtttattct gttaaaaaaa aatcaaatta tcgggtaaac ataaggatca 5700
acccgtagtt aatatttgca gtagtatttt ttaacaatga attataataa aaaaataatt 5760
cattactatc tattataaaa cccatcttta actttaaaga agaactagat catctttttt 5820
ttgttgtgtc agaacttctt caatttatta cccacatttt atctaaaaaa aataaaaact 5880
acatcatatc ttgtttcttc atcaaattat cataccattt atagggtgta ggttgggaac 5940
attccatcat gtggtaatca gggtatttat atattttttg atagtaacat ctatttggca 6000
gatgtattgt ccaacaatca tgtctaataa aatcattttc acctatgggg gaatcatctt 6060
aaaaacctta ttcctacaga ttccattttg acagtcctag caaaagtcac aatattttcc 6120
atgagtacac caatgttcaa gctctctttc gggaggaatg ctgccaattt tatgtttttt 6180
agcttctaac tctctgtaca acatcagttg ggaaagcaga aagaagatta ccaggagaac 6240
cattaaatat ataatagtct gcaaactacg tttgcgaatg taatttgcaa ctaaaacaca 6300
acccacaagg taaaatccat aagttaataa cttttgccat tttcgtatga cagcctcgtg 6360
ccattcatgg ttgtgttgtg ggcattctgt tcggtaaact tcatgaggct ttatagaagt 6420
tacatagtag gtacagaatt cattgtgacg aaaaacactg cagttagcta tgtagtcatt 6480
ttcaagaatg ggagaatggt tttcaaagac cttattctta cagatgccat cttgacagtc 6540
ccaacagaac ctacaatgat ttgcataggt gcaccagtat tcaagctcct tttcaggagg 6600
ggttcttgtt agatccagga gctctagctc atatgtataa agaagagttg gaatggatag 6660
taaagtaaat atttgcagac caagcatggc tacttgtgaa caagtggctg ctcgtcaaca 6720
aatagctgtt tatcagcaaa tagctgttta tcagcaacaa ctaattatca gcaaatgctg 6780
cttgtgggta agccaataaa taggccatac ccttgaaagg agaattcagt ttgataaaaa 6840
aaataacgag ttttctaata acccggtcaa gcatttaata aatgaatagc atcacacgtc 6900
tgcatcgtgc attctgcctg gaaaatgggc ccatctctaa tatatttaca ctgacggtga 6960
atcatacagt gttccatggg atagctatgc tcctgtacag gaggcatatc ttttagaact 7020
ttattcttac aaagaccatc ttgacaagcc cagcaaaacc gacaattttt cacatattga 7080
caccagtatc taagctcctc ttccagggga ttgtcggtcg aaaacccctg tagactagct 7140
aggccagcta gcagcaagcc gaggtaacta aagaacctca ttgtagtgtt atattacgaa 7200
aaaacatgtt aaaatttgga aaaaaaagcc ctttttatag atctggaaaa aaattttcac 7260
aaatctaatt aaaagcctta cagatcatcc ttttcataaa ttttcattaa caattggtgg 7320
gggcggttgt gaggtactgg atcagaacaa tccataacat ggtaatgtcc atttccttca 7380
ccatatgtac actggttata ccagcgagaa acctcacaag atgtcaaata actgttctca 7440
acaatcaatg gcatgctctt attcaccttg ttcttgcaaa ttccatgtgc acattcccag 7500
caaaacttgc agttttccat gtaagtacac cagtatccaa gttcttcttg tggaggatta 7560
tccgttgaac gaagatgccc tcctgcctga gtaggtagtc ctaagacctg attggccagc 7620
aggccaagaa tttccaagaa gatcaccaac attgctacgg ctggctgaac agctggcaga 7680
tagctagcta attagcaaac caagtgactc gccctctcta ctcttaatat gagaatttaa 7740
gattcggtcc ggcttttttc ccatgtttta cagggaaaag gtatttttag cctatgaatg 7800
tacatggttc cgcacattaa aaaaaaataa aagaaattat ttaatattgg ctgttatttt 7860
ctttcaacta gcaacaagcc aggtaactaa agaacttcat tgtagtttta tattacggaa 7920
aaggttaaat tttggacaaa aaaaatcata tctaattaaa aatcctcaca gatctttctt 7980
ttcataaatt ttcattaaca attggtaggg gcggttgtga ggtactggat cagaacaatc 8040
cataacatgg taatgcccat ttccttcacc atatgtacac tggttatacc agcgagaaac 8100
ctcacatgtt gtcaagtagc tgttttcaat aatcaatggc atgctattat tcaccttgtt 8160
cttgcaaatt ccatgtgcac attcccagca aaacttgcac ctttccatgt aagtgcacca 8220
gtatccaagt tcttcttgtg gaggattatc cgttgaacga agatgccctc ctgcctgagt 8280
aggtagtcct acgacctgat tggccagcag gccaagaatt cccaagaaga ctaccaacat 8340
tgctacggct ggctgaacag ctggcagata gctagctaat tagcaaacca agtgactcac 8400
cctctctact cttaatatga gaatttaaga tccggtccga catttttccg atattttaca 8460
agaaaaagat atttttagct acaaatacac ttcatatatc cctaaaaaaa acaaaaattt 8520
atttaatttt aactattatt ttctttccac tctctcttta agattttgta aggattccag 8580
ggctttggtt cagaacaggc cattacatgg tgaatcccct gtcctagatc atacatacat 8640
ttatttagcc agcgggaaac tatacatgat tgcacatact cattttcaag aattgttgta 8700
ttctccaatt tgccctcaca aaggccattt tgacaattcc agcaaaactt gcagttttct 8760
gtataagtgc accagtattc aagttcttct tgtggaggat tatccgttgg atgaagttgt 8820
ccagctggtt gattaggtag ccctaagacc tggttgcaat tcatggtatg gtagataccc 8880
ttatctaaat catacataca tttatccagc caacgggaaa ccagacatga tttcacatac 8940
tcattcttgt aaattactga cccatctatt ttgtttatac aagtgccgtc ttggcagtcc 9000
cagcaaaatt ggcaactttc catgtaggca caccagtatt cgagttcttc ctctggaggc 9060
tcctctgttg gacgaagttg tccaacgagc tgacttgaaa cctggctggc cagaaggcca 9120
agaattccca agaagatcac caacattgct acggctggct gaacagctga ctgaatagct 9180
agccaattag caatccactg tacttttcat aagatcattt aagattcggt cggcattttt 9240
tcaatagttt gctaggaaaa aatttttaat tttatagatt cacactactt cattctcatg 9300
cttaggaaaa aaacaaacta aatcttacaa tgtatctgga tctaatgaga agctagaatt 9360
catctttttt caaatccttt ctgggatgtt cattcttttt ccactccttc cttgcaattt 9420
tataaggatt ccagggcttt gggtcagaac agttcatgct atggtaaatg tgctcctcca 9480
catcatatct acataggtca ccccagcggg aaacctcaca atattttaca tagtcattct 9540
caataatact tgtggagttg tttccccaaa ccctgctggt acaaatccca tcttcacaat 9600
cccagcagaa ccgacagctt tccacataag tgcaccagta tccaagttca ttctctgggg 9660
gttcaaatgt tagaggaaga tgtccaccta cccgagtaga agtggaggat gaaaccaggt 9720
tgctactggc agcaggccaa taattcccag gataatcacc agcattgtgc tcaaccagca 9780
acggctagca acgactagca actgactagc aatagctaga aatggctagc aatcagtagt 9840
agctaacgct ctactcttta taagaaaatt taaaattcga tcagattttt ttagaattga 9900
gaatgagtaa aacgcttata ttctttttct agctagaaaa aataagctag tttaagatag 9960
gatttccctt actaacggtt taatttttag caaaggtata ggtaaaatac acttgtactt 10020
agctgcaaaa aaataagctt atggcgtata agccgccata agtttattta attaaaatgt 10080
taaactctgt gataagactg gaatcttagg caggtttgat gtggagaaca gcatgaaata 10140
caagagtgcc tgttacacga ataagttctc tcaaaccggg gatggtcata ctcacatcta 10200
tgaaatcctg gtctaggaga ttcatttgat gcatgatggc cgcacccaca cttatgagac 10260
actgaagaac taaagggttt aattttgatc tgaatggtac tatataggat gatggcaatc 10320
catatcaaga ttagagcaat caaaatcacc tcctcaagaa gcatgatgta gccttaaatc 10380
ttagactgct ttaaacctta ggccctcact atctttaatg aaggagttta aattttgatc 10440
cctttttcaa gacccattta gaagaaaaaa ataaagttta tatcaatcta attcataagt 10500
catctctttc ataaatcttc atgtattctc tatgtggata agtatgggat gttggatttg 10560
cgcagtccat ttgatgatct gtatggtttt tgggtccttc ataataacta catataccat 10620
tccagcggga aaccgtgcaa tttataatcc agtcattttg atgaataact ggccaatctg 10680
tttgaatcct gtttcggcag ataccgtgga cgcattccca gcaaaagtca cattggtttg 10740
cgtaagtgca ccaataaact agctcatgtt caggaggata acgggttggt agtaaatctt 10800
ctaatttacg tataggagcg gcttgaagga caaccacccc cagtagtact agaatcagta 10860
cctttatagt ggccacccta cactagacct ctaagttgaa gacaaagaac taaaatttag 10920
agccgtttaa ttactactaa taattatatt ttttattgtc tacaatagga ttctattaaa 10980
aaataatgat ttttaccaag aaatattttt ataaaaaatt aatatatttt gtaataaact 11040
ttatttccaa tgactgttaa aataaggaaa ctatccttag ttagtcgagg aagatggtta 11100
ggttatttcg caatccgata aaatgtttat tttatcgtag gtctcgtaaa atccaggaaa 11160
aaaaattacg gaagagttta aaaaagctaa atttttacca ccctccagaa gattgttgtc 11220
aaatatatcg tttgctagaa aatgttcctg gaggaactta ctttattaca gaaaatatga 11280
cgaatgattt aattatggtc gtaaaggatt cggtggataa aaaaattaaa agcattaaat 11340
tatatcttca tggaagttat attaagattc atcagcacta ttatattaat atttatatgt 11400
atcttatgag atatacccaa atttataaat atcccttaat ttgttttaac aaatattata 11460
acatctaagt aaatattctt ggaatggatt ttcttataga atggttacag gatatgtcag 11520
cgacaggctt aataacaaat ttgttaatat ttttttgtta aataaatgaa caggccacca 11580
tttaatatta cccgttgcaa aataagaaaa aaaaacaaac ttatagttac aaatcatctt 11640
gattaatcac atgtcgtttt aactcaatga accattctaa atctttgggt tgtgaacaat 11700
tcatgttatg ttgatagtgt atcctaaagt gagcttcata catacaccgg tcatgccacc 11760
gggaaactgt acaattaaca atataatcat tttgcgtaat aatagggtgg tcactaaaca 11820
ctttattttt acacattcca tctttacagg tccagcagaa gtcacagtgt tttgcatagg 11880
tgcaccagaa cttgagatcc ctttcaggag gcctacgcat ttgcatcgga ttatctgtgg 11940
aaagaggtag gttcattatt atgttcgtca tcaaaattcc taaaagaaca tagaagccaa 12000
gaaagataag cagtcttgta gcggcttgca ttcgcattcg tgagtattgt ttgcgaacat 12060
agcttatgag agcaatggta gctatcatac aaagacaagt atgtttgata ttctcagtgt 12120
caatgaccct atcctccttt atttgcatta actcatcaaa ccaatcataa tatgtgggat 12180
ttgtacagct catgatgtga aagcggcgta tcctagagtc tgtaaagtag ctacatcttt 12240
cattatagcg agaaacccta catatttgta tgtaatcatt ttttttgatg agagggtgtt 12300
tttcaaaaac cttattttta caaaccccgt gtcgacaatt ccagcagaag tcacacgatt 12360
ttgcataggt gcaccaatac tcaagctctc tctttggagg tctccgggtc attggtaact 12420
ctcctgttcc tggaaaagat tggctttgaa tgaccggctg catgaccgcc agtaccaaaa 12480
ggaacacaat caccttcatg gctgcaactt ataagttgca acttatgggt tgcaatactg 12540
caacgtatag gttgcacctt atagatcgcg actcaaaagg tatgaaaacc ttaccctcaa 12600
tacagaattt aagttttaat cctgataatg tatctgttta tgaaaaaaaa ttttttttac 12660
tcatgtatga attcttatac gaatcataat atgtaggctg agaataataa ttcatatacg 12720
gtgttgcggg ctcaataaaa attttgttac cacaaaaaat aaatgctgga tttttaagat 12780
atatatctat taatgactaa accctttata cgctgtaggc tgaaaacaat ccatataatg 12840
aatatacggt gatttgggtt taataaaata catacaacgg tcaaaatagc gggcaatact 12900
acattgacta atataatcat tttgtttaat aagaggcata tcatcccaca ctttattttt 12960
acaaataccg ttcctacatt cccagcagaa atcacagtgt tttccatacg tgcaccagta 13020
ttcaagctct cttataggag gcgtataagt ccttggtaaa ttttgtttca tataaaagat 13080
ggaaaggggt cgatttaaac ccggctgaga tagccaaatc aaaatacata aaagagcaag 13140
tagtttcata gtggtattta gatgtaaatt tttatagtat gcaaatacaa tgtaacctac 13200
aaatacaata ctaaatacaa ggtaaaaaca acaatgtctt ataatgattg gccaataatc 13260
accccccccc cccccatttt tccatgaata tttcatttcc tgtatagggt ctaggatgtg 13320
aacactccat gttatgatga ttaggcattt taactgatat ttcataaaaa cacccccagg 13380
aattgcgatt aactatacag tttacaatcg aattcatcga attagactca tttgttatct 13440
tatttttaca aatgccattt tgacaatccc agcagaagtc acaattcttt acatacgtac 13500
accaatatgg aagctcctcc ttaggaggat gctgggttct tggtaattct ggtaattcat 13560
gtgcaagaat gaggactgag tagcccaaca aaagtcctag aaccttcatg ttgtgtccaa 13620
atggcacctg tcattttaaa aaagatttaa attttgctac cgcaaaaaaa aatccagtat 13680
gtattttttt aatacatata attattgaag tcttataaga taaagccgag aacactatat 13740
tttgtataga tgatgtatcc ggtattcaaa ctctcttata agtacatgta ggaaatggtc 13800
aattattcaa gattggctga gataacaaca aaaccaaaat actcaaaagc ataagtaatt 13860
tcatggttgt actcagtcgt agatttttgc agatcgcaaa tgcaacgcaa ccagcaaata 13920
caaagctaaa tacaaggtaa aaacaataat accttataat gattggccaa ttcttatccc 13980
tccatttttc catgaacatt tcatgttcat aaagtctagg atacgaacaa catttcatgc 14040
tatgatgatt aggtatttta agtgatattt cataaaaaca ccacggggtt gttggtgatt 14100
gataggtaag aataaggatg gttgaataac ctagtaaaag tcctagaaaa accttcatat 14160
tgcgttcata ccacagatgt tatttaaaaa aaatataaat tttacagtat gtgatataca 14220
cataccacaa aaatgttctt atattaacta aaatatgtgg gcagagagca attcatataa 14280
tgaatatatg gtattttagg ctcaataaag tacatacaac gatcaataaa acgggtaata 14340
ctacatttac tgatgtaatc attttgaaca ataagaggca tatcatccaa aaccttattt 14400
ttacaaatac cattcttaca atcccagcag aaatcacagt gttttccata cgtacaccaa 14460
tattcaagtt ctctcatagg aggcgtatag gtccttggta aaatttgttt cgtataaaag 14520
atggaaaggg gtcgatttaa aactggctgt gctaaccaaa ccaaaatact caaaagaacg 14580
aaaagtttca tggttgtact cagacgcaga ttcttacaaa gcgcacatac aaagcagcct 14640
gtatatgcaa taccaatgat gaaatagaga cagtattgct ttatagataa ttgttgatgg 14700
tcaccccccc ccccccccca tgtttgcatg aatatttcat ttcctgtata gggtctagga 14760
tgtaaacatt ccatgctaaa gtgattaggc attttagatg aaatttcata taaacaggat 14820
tgagtcttgg aatcacggaa aactctacag tttacaatag aatgattgga gtcaatgaaa 14880
cgagattccg ttatcttatt tttgcaaatg ccatcttgac agtcccaaca gaaatcgcat 14940
tgtggtacat acgtacacca atatgaaagc tcactcttgg gaggatgctg ggttcttggt 15000
aagtctggta attcatgtgc gagaatgagg actgagtagc ccaacaaaag tcccagaaga 15060
accttcatgt tgcgtctaaa tgacacctgc acttacaaaa aaaaatttaa attttgaata 15120
taacacaaaa aaaccacctt aaaatttctt atattatttc ttggatctgc cccgacgtca 15180
tacaatgtat taaaattata gaccaatcat ctttttgtat ataggctaat catctttata 15240
tatagatttt agatgtttgc ttgttgtatc aacttaactg ctagcgaaga aaatggataa 15300
aaactttctg tatttttata ggttgaaatc attttatgca catcgctagg atctaatatt 15360
ttattttgaa gaaccgaatg tgggcttaaa atttttttct tagaaaaaag tagaatcata 15420
atattgctat gtttttgttt aatgatttct tgtatctttt ttgtatacgg gttggcaccc 15480
aaacctatac aaaaatatac attactcaaa taactacctt ctatacataa tcttttttcc 15540
ccacgtattt tcctatttat ttccctattt atggaattaa aggatatcaa tctctctaag 15600
gcacggtcaa ggtctgcgcc taaggcaaaa caataatata tacctaattt attcccaggg 15660
cgtgcacagg caagaaacat catgacgttt agccctaaac gtatattttc ctgaaaatac 15720
gcatgatgaa cttcatcaat attacctaag tatatggccg tttgtaaacg ccaaagatct 15780
aaatgaggaa attttttact aagataatga ataggttttg tgagattaaa atctatggcg 15840
aacttatacc aaaattttaa tacaagtgta tttctcgtca tttcttcttc tttttcatct 15900
aaatataaga taaaacgatt gtaaacaaag tctatcaata ggtgaaaatc attgctatta 15960
aagctgtcga gaatcaaaat attgtcataa taaatttcga tcgccagtaa aacctttttt 16020
cgtttgacga gataaacaaa catattatac aaccctacat ctaaaaattc tggattggct 16080
cctagttgga tacacaggtc tttagtctgc ttcgttttgg cacacatgat gccaaaatta 16140
atatcagcac cccataaaac aaataacttg attagatcag tctggttttc cttcacagct 16200
tttactaagg ctctgtcaag ctcatagctg tcgacatcag agcatgacat agagccaccg 16260
gttaccattt tacattgctt acaaaaacct atgggtccgt tttcccacca tagtccaagc 16320
tgttgtagaa taaaaatatc atcctcatga taatttgaaa aagccttggt ttctatcaag 16380
actttttttg taagaacctg taaagagttc atcgtattat tatgaataac aggagtaaac 16440
gtaatcaatt ataaaagtga ttttttcgaa aaaaacttta gatggttgaa aatgataatg 16500
tacatgttca tacaaaaaat agatgcagtg atgtctaaaa tcaaaattta attttctatg 16560
taaaaagtac agacttactt atttgggtta aattgtttat tttaaacttt aattaaccgt 16620
ttgagttagc gatgtttgat ttatcttcca tactcatccg gggggggtcc ttatagctct 16680
gacattattg tggattattg aatataatga atacttcata gatgctaaac attttaatag 16740
tagttctgag gcttaattgt actctataaa tttataaaaa ctttttgatc aaaatttaat 16800
ttcttataaa aagagtacag acgtcgcttg tttaagcttc atcatgtttc attcattact 16860
ttctacaatt acgggggggg ggagtcccct catagcttta gtattgctat ggtttactaa 16920
ttattatgta gaatttatag aagcatatgt acctgaaagt atacctactc tataaaatta 16980
aataatttca gtatattttt tttatgaata gaacggaaat gatataaaaa taatttaata 17040
ttgcaaaaaa aattcataat gttggtatgt attataaaca taatagcatg tgtaatttat 17100
aaactgactc ctctatataa ttattagatg aggtaccaac ctacttatga tatgccgatg 17160
atagatattg tatactataa aacaaaatta ttttaaatgt attcatggat acattataac 17220
atttttaccg caaattgtct ctcagcgaag aaaatgaatg aaacgtttct gtatattcat 17280
aggttgaaat tattttacgc acttcactag gttctaatat tttcttatga agtattgaat 17340
gggggcttaa aagtcctttc ttaaaaagaa gtttcatcat aacattcttt tcttgtctaa 17400
gaagagtttc ttgtattttt tttgtataag gattggcacc caaacttata caaaaatgta 17460
cattactcca aataccataa tttgaaaaga aagttatttc cctatttact tcatgattaa 17520
tgaaacctat caacgtctct aaggccgtat tgatatttgc gcctaaggca aaacaatagt 17580
atatacccaa tttattttga gggtacatac aagcaagcga catcatgtca tttggatcta 17640
aacgtatatt ttcctgaaaa tatgcatgat ggatttcatc aacattacct aagtatacag 17700
ccgtttttaa acgccaataa tctaggtgag gaaatttctt actaagaaaa cgaataggtt 17760
ttataagatt aaactctatg gcgatcttaa accaaaattt taatacatat gtatttttta 17820
tcattttttc tttttcatct aaatttaaga taaaacgatt gtaaataaag tctatcaaca 17880
cgtaaaaatc atggctatca aaactgtcga gaatcgaaat attgtcataa taaatatcta 17940
tagctaataa gaccttttgt tgtttaatta gatcaacaaa catattatac aaccctacat 18000
ctaaaaattt tggatcagct cctagttgaa tacacagaac tttcgtcctt tccgtcttgg 18060
cacatatgat gccataatta atgttggcac cccataaaac aaataacttg attagatcag 18120
tctggttttt cttcacagcc ctcaccaagg ctctgtcaag ctcatagctg tcaacatcag 18180
aacatgacat agagccactg gttaccattt tacattgttt acaaaaacct atgggtccgt 18240
tttcccacca taatccaagc tgctgtaaaa taaaaatatc atcctcatga taatttgaaa 18300
aagccttgtt ttctatcaag actttttttg taagaacctg taaagaattc atcgtattat 18360
catgaatgaa agcagtaaat gtaatcaatt ataaaattga cttattgaag agaaatgtta 18420
aatgagtgaa atcggtgttt atgatgatgt acatgatcat acgaagaaac acgttcactg 18480
gtgtccatga tcaaaattta atgttttacg taaaaagtac agatgttaac tgtttagttt 18540
aaacataaat ttaaccttta gtttaaaccc tagttaatga tgtttaatat ttcttctata 18600
ctcattcagg gaagtgtaat gattctaata ctgttgttat ggattattaa tgaaaacttt 18660
acagatgctg gagggaataa ttttaatcat actgttttaa tgtagctata taagctttca 18720
tcaaaattta attttttttt ataaaaatac acgaattaaa ctaaagtcta aactttagtt 18780
tgactatttg agttaatgat gcttaactta tcttccatgc ttatcaaggg ggggtcctaa 18840
tagttttgat actattgttg tggattgttg aatataataa atactttata gatgctgaaa 18900
tgtttgaaaa taatagtaca tcaatgttgt aagtttgatc aaaatttaat ttctcataaa 18960
aaaggtacac atcaacattg ctcatttaag tttcatgatg tttgattcat tacttcctac 19020
aattactggg ggggggtctt taatagcttt agcattgtta tggtttgctg actattatgt 19080
agaattcata gaagcacgtt tagatagtaa tatcactgca gtgtagatta tgaaatacat 19140
actaaactaa tttcagtata ttttttttgt tcatataagt taaggtacaa aaatgattaa 19200
acattgcaaa aaaagaaaat cacaatgcta ttatacatag tgatcatagt ggcttgtatc 19260
atttctaaac tagttccaaa tgaatattgg gcaatacatc tattttttat cattatgatt 19320
tttatggtat atatgtatga aaagttagat atacatcaaa aatctcagtt ctggaattat 19380
accatgtcag gcttatctgg acataacgta caggtaacat gtaagtgtta ctaaatacta 19440
tgaagtatct attttttttt gttgtaaaaa aaagaacttg atagtatttt ttaaaaaata 19500
aaataattaa ttgtacgtca acttccttat tttattcttt aaaaataact cgtaagtatt 19560
atttatctat tttttgaaaa aatagatgta atcggtttca tcatttaggt gtgtatttct 19620
ttttagcatc tatcaagaat tcattgttta gtgatatgaa aacaatgaat gatcattatc 19680
ttctatttaa caaccaccta aataaatgaa cgtctttttc atcttaactg attaccaaaa 19740
gttattttgc gaaaaggcat acatatgatc aatatcagac ctacaatgaa tatttccata 19800
atatcccttt attgtaataa ttctattttt gcattccgat atctcatcat ctgtgctatt 19860
atatgtttcc ataactgttt catcatcaaa cataaatcct gttaaatagg caaaagactt 19920
taatcccgga tagattttta ccattttcct gagagccgtg tatagcttgt aataaatggc 19980
caaaaatatg caataaagcg tagaaagaga gtaatttttg gcataaaaga ttttgaaggt 20040
ttgatgaatg gctaaatcgc atataatata agatacgatt ttaaagcgca cctgttcacg 20100
cagatttgtt gaaaaattcg tggaaagatt taacaaataa aaggttatta atagttgctc 20160
atcattcccc ttatacgaca tcgtcagacg ctctaatatt ttactactag gcacatctgc 20220
cacatgttga acatttaaag cctgttcttc ttctgtgtta cggcaaaaga gccgtgcgta 20280
ttcaggtgaa gctccccagg ataacaacgt ccttgctacg gctaaatttt ttttgacgat 20340
gacttttatc agaaataagt ctttattttt gcattgatca ctatgcgaat ttgtatagtt 20400
gacgccgttg cattgagtac attgatataa tgttttacaa ttccagcgta gccctaaatg 20460
gtataaaaga actgtatttt cgacataagc atgctgatta acgatgtttt tgagacaaca 20520
cgtcgttaag gacaccatat tgtctccaat ttgttagata aaagtcttta ctaaaaaaat 20580
agatttttag ttttaacaat cgagatttta ttatttggat gcatcatcaa aaagatttat 20640
aagtataaga ggttgtataa gaaaaaaaat gatgttatac tatttatgtt aaaatttaat 20700
ttatcatata aaaagtacag atttaatcag ttggttaaac tatttagtta attaaactaa 20760
atagtttaac catttagtca gactacttgg ttagcaatgt ttgagctttc ttccattctt 20820
atccgggggg gggggtccta atcgttctaa tactattgtg gatagttgaa tataatgaag 20880
actttataga tgctataatg atgaattcta gtatgcctgt ataaaataat taaccttttt 20940
gatcaaaatt taattttttt ataaaaagct acagagtagt gttttattaa acgtggctta 21000
tttaaaagtt acacaatgtt aaaatctcta cttactttaa ttctttgtgg ggttttatta 21060
actttatcca tattatggct tactacttac catgtagaac ttatagaggc aatagatgat 21120
ttctacgact gaaatataga atagtccatt ttctatttgt aaaataatga tttatattct 21180
ttcctaaaaa tgatacttta tatggtttga aaacaaatat taacaacttg attttttttt 21240
ctataaataa actataaatg aaaatagtaa aactcataga gtcttataag tgaacatctt 21300
cataatgtta ctcaaacgtt ggactattaa aaaatattcc gtgtgcatta ttgcttttaa 21360
tcagtatgat tactttatac gaagccgcta ttaaaacgct tatcacacac cgaaaacaaa 21420
ttttaaaaca ccccgatagc cgtgaaattt tactagcttt ggggttgtac tgggataaaa 21480
ctcatattct tgttaaatgt cgtgaatgtg ggaatatgag tcttaccgga aaacacagta 21540
caaaatgtat taacattaat tgtctactta ttcttgccat aaaaaaaaag aataagcgta 21600
ttgttgatac cttgatagga atgggcgcgg atgtaacata tatacatctt ttaaagaata 21660
agataaaact gtcatacaac cagctgtcta tgcttaaaag caactcgcag atttcattga 21720
aggagcttca tgctatatgc tatcttttat atggtcggct tcccaaaaaa attaaacaag 21780
ggatgcgact gtgtaaaaca atggcgggac tatgtggtga acttttatgt gcatttttag 21840
ctccgtaaat gataatatgt atttaaaaca aacagatatt accaaaatat attctatgta 21900
cataatatct gggaaattat ttttttttct cataccctta aatataaaaa tattgggttt 21960
cttcactaaa ctttagaggt aaaaattttt ctttgttttg caccatcatg tatgggttta 22020
ggctgtccca gggattgttt atttgaatat ttcctaaata ggaacacaac gccatgatca 22080
tatatctttc attctggtaa gctttttgat acatcttcaa agatgccgta cctccgagtg 22140
tgtaacagca aacaaacgtc cgtacttttc catgggtcgc agcccattcc attccgtagc 22200
tcagcatctt ttgctgtatt tttttattcg ctttataaaa aaagtttttc atccattcca 22260
cgttctcata aaaacaggca cttaaaaaga gcactagggg tagtgtagtc ttattataga 22320
atgtaggaat gtatgtttta gttatttttt tcaacgcgtg ttccatacta tgttttaccg 22380
ccataaaaat acaaaaccaa taccaacttt ttctataaaa ggttttgctg tacacatata 22440
aacgagcaaa atatatttca aactctatat tctttttata aaaaaactcg agacagtcgt 22500
ttatgttacg actttttcta aatacctcaa aaacagtaat taattcactg tcgctgtgga 22560
aatgttcgta agctaactgt ttaatgtctt taggggtcaa ttcttttttt gggagcagtg 22620
gtttgagatt cggcaaaggt cgtctaaagt agtgagcgaa cttttcattc gctccccaac 22680
acaaaagccg ataagccagc atgtagttat cacgttttac cgcgtaaata agcaaatagt 22740
ttatattgat acatgtacca tgttgctgcc cgtttggaca tatgttgccg cattctgaac 22800
acttatgaat gagatcatag ttcttacaac ataaccccaa acgggttagt acttctttgt 22860
cacgttttaa aaactcgaca tgattcttta atgttaatgc tttgagcgca atgttaaata 22920
aactctgcat tttattaaaa tgaggttagt atcatgtttt agtataaaat ttagcggctg 22980
tttacataat gctaaataaa cttaacgttc ctactaaacc aaaaaaaatc aaattgacta 23040
agtcatagag aatttgacga tgttggtagg taatttttta acatggtata tattttttta 23100
gggtcggtta tattaggtaa taaaagagga cgtgccgtta aagtattttg cttaagatcc 23160
tttagatcct tacaaaaata tagattgttc gtctgatgat gccactgtgt tgcagtgatg 23220
gcttgatcaa tatcacctcc caagacaaaa cagtagtata tcgttaaaaa gttgtaatct 23280
ttcatacaag ccaactgcat cattttatcg atgtccatat gaacgatctt ttgctcgtat 23340
atttcatgaa ggtcaaatac attgttgaag taaatggcgc acatgagtcg ccacatacta 23400
aggtgcccat atgtttgata gaaaaaggag atagctcttt taagcttata ttttactgct 23460
atggcatagc agtatttaac gaatacgttc atgggtacat tatctaagat ataaaatatg 23520
aaaaacttta actctcgatg aatctcttcc cccatttcct gtacatttag agcttccaac 23580
ataggatttt tatcaaatat ttcatgacat aaaataatgt tattgctcgt tttatgacgc 23640
attaaaccgg tgaaaatttc cttattattt aaactatctt tagctcctaa ctttcgacac 23700
agctcctgag tttgttccgt cctagcacag gtcagcccat aataaatgtt tgctccccac 23760
tcggtgaaca gccttattac gtcatagtta ttttctttta tggccatgat taatgccaca 23820
tcaagatgaa gaagttcccc cttaaagggg gttgagctta aaataacgta attacagtag 23880
tgacataagc taatgggctt gttttgccac cataagccac aatattttaa aatataatga 23940
tactcctcag gcacgctctg tttggccaca gcctttttgg ccagggtttg caaggagagc 24000
atgataactt cttgaaaaaa aaactcaaat taagttccta cttttttaaa atattagtat 24060
ggacagatct accatcatat gaaggaattc tttcatcgtt aaacactgaa gagataatac 24120
tttcatcgta tagagaatat catgtcaatc catatattga atgttatata tcattaaacc 24180
catcattaat atagtgttta tgtgctatgg acaggttttt tgaatgataa tcttttaaca 24240
tacgttttat aacttcggga tcagtttctt ttaaagataa agaatcattc atgttataac 24300
aatttaatga taacatgctg gcaatgaacg agttgtcttt ttgatgcgct agagtctttc 24360
cctcctcaaa ggcattggcg cctaagtcta tacaaaagaa tatgtttccg atattataga 24420
actgaataga atgaaacatg gcctgattga tatcagcccc taagacgacg caacagtaat 24480
aaatcgttaa atagttatag ttcttgcgac aggcccactt tagcatttca ttcatgtcta 24540
tgcgaatcct ctccttttcg tacacttcgt gaagttcaaa cacattattg taaaaaaggg 24600
cgcacataag ccgccaccga tgtagatgag catatctctg ataaaaatag caaatcgcct 24660
ccttaaggtt acattctatt gccatcgcgt accaatattt agtaaacatc tcgcttaata 24720
tatcggtttc taccattaat ccctccagtt gttcataaat cattcccttt acttcaaaac 24780
gatttatggt atctaaaatg ggattattag aaaatacctc atggcagaaa atgatgttac 24840
tgctagttag atcacgtttc aatgtgtaaa aaaatcgtaa aatttcctgg tcatttaact 24900
gttctttggc acctagctgc ctgcacaggt ctcgggtgtg ctccgtgttg acagaaagca 24960
aaccgtagtt gatgtttgca ccccactcgg tgaacaattc tattagatcg tgattgtttt 25020
cctccacagc tttcaccaag gccgcgttaa gatttgtgcc gttcttaaaa tacggcgtcc 25080
atattttctt ttgatgatac atgatagggc cattatgcca ccatagaccg cagcacttca 25140
aaaaatgagg atggcatttg gccggatact ggctggccag cacctttttg gtgagagtct 25200
gcagagagag gaccatattt cttttttttg aaaaaatcaa attaaaaaaa tcatgcttgt 25260
ttagcataca tgtaatattg ttataattac gttataatta cgttataatt acgttataac 25320
tatattataa caatggtata acaatggtat aacaatgtta taacaatgtt ataacgatgt 25380
atcattgatg tcatcattca actaggccaa catacttttt aatttatagt tttttaatag 25440
atgatatatt ttgctaggat ctgcttcttt taacgttaat agcgaggagt ctgcactata 25500
aatgtctaat gataaatgat gagatatcaa atagtaattc cgttgctctg ctagggcctt 25560
tgcctcttca aaggcgtcgg ctcccagatc tatacaaaag aacaagttat ccatattata 25620
aaatcgtacg caggcaagca tagctgaatt aatattagct cctaagagaa aacaataata 25680
tatggttaaa aaattgttat cttttgtgca ggccatccgc atcatttcat ccacgtccat 25740
gcggatcttt tccttttcat acaaattatg taggtcaaac agcttattaa aacaaagagc 25800
acagattaac caccacgtat ttagatactt aaaatgttgg taaacataag aaatggcctc 25860
cctaagatta tcctgcaatg ccactataaa acagtatatc gttaacatat caccatccga 25920
catattactt aatatgtcgg tgtcttctac taaccttttc aacttccaat atatggatga 25980
ccttatttcc cttataatga cataggctgg aaagggatta tcattaaaaa gtttaagaca 26040
taagataata ttactgctag tagtgccagg gtgtattaat ttaaagaaca tgtgcataat 26100
cttcttttta tccacgcggt acttggctcc taattcccag caaaattctc gaacaggcgg 26160
cgtattggcg caaattaacc catagttgat gtctgcgccc cattctgtaa acagttttat 26220
taactgatag ttgttttcct ttgtagccaa cattagtgcc gtattaaggt ccaagccgtc 26280
tgcaaagctt ggcagcttta tcagcatatg tttgcaatca agggaaattg gggccttata 26340
ccaccatagt ccgcagcgtt ctaagataac atggtactca atagatactt gctgtctggc 26400
tagtaccttt ttggcgaagg attgtaagga aggaaacatc ctgtttcttt tttttttaaa 26460
aatcaattat ctttgttcat aatcaagaaa aatccccata tttattgagt gataattttt 26520
taacatgcaa tttatttttt cagggtccgt aacgatcgac aacagagaaa taaccggatt 26580
gtaatgcttt aatgataagg catgggctat cagataattt tccttttgtt ctgccaaagc 26640
tttgccctcc tcaaaggcat cggcacccag gtctatacaa aagaacaggt ttccaagatt 26700
atagttttgt atggaaacaa gcatggcttg attgatgttg gctcccatga taaaacagta 26760
gtaaatggcc gaatagctat aatcttggat gcaggctatg tgcatcattt catcaatatc 26820
catgcggacc ctttctattt cgtacagctc gtgaaggtcg aacacgttgt tgtaaaaaag 26880
ggcgcacatg agccgccacc tatgtagacg cgggtatttc tggtaaaagt agcggatagc 26940
atctttgagg tcatagtcca ccgctatcgc gtaccagtat ttggttaaaa cagtgctaaa 27000
gctatcatca tggtccagca tgaaggttat ctccatgagc cctcttaact cccacatgat 27060
ttcccccctc agatccagat tatctataat ccttaaattg gggttattgg aaaacacctc 27120
gtggcaaaag ataatattgc tactggtttt atcgcgcgtt gtatcaaaga aaatttttaa 27180
aatatactct ctttctaaat attctttggc tcccagctct ttgcacagat cacgggtatt 27240
ttccgtgaga gcacaaatca ttccatagtt aatatctgca ccccattcag taaacagctt 27300
tatcaagtca tgattattct ccttcacggc tttcatcagt cctatgttta actcgatacc 27360
ttgactaaaa caggttgacc ttataaataa tttattgcgt cgaatatgaa gcataatggg 27420
gccattatgc caccacaggc cacaacactt caggacatga tattgatcta ccggtataca 27480
ctgcccggcc agtactttct tcgtgaggga ttgcagggaa ggcaacatgc ctttccatcc 27540
tttgacggaa atcaaattat ctactaataa ctatcagtgt ttatattaag tatttagata 27600
ttatcccggg ctggatacgt agtatcgcta ttcacatgta cttccaactc tagccggagc 27660
ctgcagggtc atttattttt aatattgatt cttttttgta tttaatcatt tagagaaggt 27720
catcatagga gccagatgtt ctctctccag aacttatgtc gaaaaacatt acctaaccgt 27780
aaacttcctg aattttttga cgaatatata ttacaactgc tgggattata ctgggaaaac 27840
catggaacta ttcaacgagc aggaaacaac tgtgtgctta tacagcaaca taccctcatt 27900
cccgtaaatg aagccctgag aacagcagca tctgaagaaa attatgagat cgtgagcctt 27960
ttattagcgt gggaggggaa cctttactat gctattatag gggctctaga gggcaaccgc 28020
cacgacttaa ttcgtaaata tgatgaccaa atcaaggacc atcatgaaat tctgccattc 28080
attgacgatc cagtcatatt tcacaaatgc catatcatgc ggcaatgctt ttttgattgt 28140
attttatatc aagctgtaaa atatagtaag tttcgcgttc ttctttactt taaacataga 28200
ttagaggatg atttgccctt cactcattta cttattgaaa aggcatgtaa agatcataat 28260
tatgaagtta ttaaatggat atatgaaaac ctacatatct acaatatgat agataccttt 28320
gaatgtgcta ttgcccataa ggatctacat ctatattgtt tggggtatag atttatatat 28380
aacagaatcg tacccgataa gtatcatcat ttagatattc gcatgctttc aagcctacaa 28440
ctcctacata aggtggcagc caaaggatac ttagatttta tcctagaaac cttaaagtat 28500
gatcataata aagataatat aaatattatt ctaacacaag ctgcaaccta taaccataga 28560
aaaattttaa tctatttcat tcctcaatca acccacgcac agatagaaca atgtttacta 28620
gtggcgataa aagcaaaatc ttccaggaaa accttgaact tactactgtc tcacctaaac 28680
ctttccatca acctcatcaa aaaaataagc cattatgttg ccacttacaa ttcaacaaat 28740
ataataggca ttctgagtat gcggcggaaa aagaagatat atttagatat catattgaca 28800
aaatttgtaa aaaaagctat ttttaataag tttgtcgttc gatgtatgga tacattttct 28860
ataaacccgg aaagaatcct taaaatagcc gcgcgaataa ataggatgat gttagtgaaa 28920
aaaatatctg aacatgtttg gaaaaatcat gcggttagac ttaaatacct taaacatgcg 28980
gtacacacga tgaagcataa agatgggaaa aatagactca tgaactttat ctatgatcgc 29040
tgttattacc atatgcaagg ggaagaaatc tttagcctcg caagatttta tgcaatccat 29100
catgcaccaa agttgtttga cgttttttat gattgttgta tcctagatac gatacgattc 29160
aaaagccttc ttttagattg ttcacatatc ataggtaaaa acgctcatga tgctaccaat 29220
atcaacatcg tgaacaagta tatcggcaac ctgtttgtta tgggagttct tagcaaaaaa 29280
gaaatcttac aggactatcc atccatttat tctaaacaat acatgcctta gtttattttt 29340
tttgcggccg aaacattatt cttaccctag aaaacgctta tagtcatctt aaatcatagg 29400
taaggaagat catcatattt tttgaaacgt aattttttaa cgcatgatct atgatttcag 29460
ggtccgtgct tttaggcaac ggggtggtgg ccggactata aatctttagg gataaaatgt 29520
tctttataag ctcataccct tcccctaaag ctgtagtacc ctcttcgaaa acatcagccc 29580
ccagatctat acaaaagaac atgttttcta tattatagta ctgtattgag ctaagcatgg 29640
cttgattgat gttggcgccc aggacatagc agtagtacat ggttgaaagg ttgtggtctt 29700
tgatgcaggc gatccgcatc atctcttcta tgtccatatg gatcttgtcc ttttcatacg 29760
cctcatgaag gtcaaacaca ttattaaaac aaagagcaca tgttaaccgc cacgtattca 29820
ggtgtgtata tttttggtaa aaatactgta tggcctcttt caggttatag cgtatggcta 29880
tagcgtacca gtatttgagt agtaatgtac tgagcgaaaa ctcattattt agcagatcgg 29940
tttttactat taactccctt aactcccaga aaatttctat cctcattttt atattattta 30000
ctttttgtaa tatcggattg ttggaaaaca cctcatggca taaaataatg ttactactag 30060
ttttatgaaa ctttagatct ataaaaattt gtaaaatttc ttcttcattc aaggtttcct 30120
tggcacctag ctctcgacag aggtcccagg tgtgctccgt gttgacagat accagcccgt 30180
agttgatgtc cgccccccac tctgcaaaca gttttataag gttgtagttg ttttccctta 30240
cagccttcac taacgccgta tttaggttta agccctcttt aatacctgct gattttatga 30300
gccttaggtt atgatcaaac gtgatcggag catcatgcca ccataggtca taacacttta 30360
aaagataatg ttggttcgtg ggcacgcatt gtccagccaa cacctttttg gtcagagatt 30420
gcagggaagg caacatgtct cttcatcttt taaaaaaaaa tcaaattaat tagccgaata 30480
aatttttctt tcgagggctt tttaaaagag ctctttaaga gctctttaag agctttttaa 30540
gagattaaaa aattattctt gctggcattc tgccaagtat gcggcattcc tatcatctat 30600
agtatattat gagaatattc ccaaatgatg gataagtttt ttgatttata atcttttaat 30660
aaactgctta tttcttcggg gtcctttaag tttagtggca aggaagcatc tgagctgtaa 30720
atatccaaag ccaaactatg gctcagaaaa ttataacctt tttgttccgc tatggcacga 30780
ccctcttcaa aggcattacc acccaaatct atacagaaaa atatattacc gatgttataa 30840
tattgtactg aagtaagcat agcttggttg atgttgcccc ccagcgcgta acagtaatat 30900
attgttaatg gattgttatc cttggtagaa gccagacata tcatgtcatg gacgtctatt 30960
tggatgtttt ccttgtggta catctcatga agctcatata ttttgttata atacaggaga 31020
cattttaatc gccattcatt aagatccgta tatttctcat ctagaaaaca aatggcgtcc 31080
ttacaatcgt attgtactgc tttggcgtac caatacttca ctagtaaacc atttaactcg 31140
tccgtttctt ttatttctat gagcccccat agtcttttat aaattaagcc ccttaattgt 31200
ataacaaatt tgttttctaa aataggatta ttcataaaaa tttcatggca caaaataata 31260
ctgccgctgg ttttattgtg cattatcctg gtaaaaatac ggaaaatatc gttgtcctct 31320
agagtttctt tggcgcctag ctgtctacac aactctcgga tgtgcttcgt attgatagaa 31380
agcaaaccat agttgatatt tgcgccccac tctgtaaaga gctttatcag actatagttg 31440
ttttccttaa cagctattat taatgccaca cgaaggtcta tatcttctcc taaaaatcct 31500
gattttattt gtattcggcc acgatccata caaagcttga gaggagcatc atgccaccat 31560
aggccacaat atttcaaaat gcagtgttca tctattgaca aacactggct ggctatcgtc 31620
tttttgacga gggtctgcag agagagcggc aacgacatgt ttctttttca ccaaaaaaaa 31680
atcaaatgtt ctcgtcttta aaggttaatt catgttctta aaatgttcat ttcatgatag 31740
tgattaataa tatggtttaa taacgctaga aggcttgttt ataagacagt cataagcagt 31800
ctataagaca gtctataagc agtctataag acagtctatg acttagtcta taactataat 31860
ttctggatgg gctgtaagat actcttcggc tcgtttcaga ttttttgaag tatatgtctt 31920
tagcatatca tatatttcct ggggttcggt tacatctaat accaaggtca catcacggct 31980
gaaaagctgc tttactaaga aaatgttgct caagttatac atataagctt tgtgcgcaat 32040
gagttgtgcc ctatcaaaat cggcagcccc caaatcaata cagaaaaaca tgtttaaagt 32100
attattgtta tagatagaaa gattcatgcc ataatcgaga ctagccccca acctatgaca 32160
gtaataaatg gccgcgtaat ttttttcccg caagcaagca aatttcatca tcagattagg 32220
gctgatgcaa atctcttttt cacgacacaa ctcgtgtatg tcaaaaatgt tattaaaata 32280
aaggctacaa gctacccgcc aatagaggtg atttttatgc cttttataga aatagtgaat 32340
agcctttgta aaattatgtc gtaatgccag ggcaaaccaa aactttgtta ataggtggtg 32400
cgccgtatcc cccgtcaacg gaatgtttga acaggtgtac gtaactgtgt ctaaagtggt 32460
tctagttacg gtttccaaga gtggattatg acaaaacatg tcataaccca gcagaactcc 32520
tgcacaggat tttagcctgg ccacttcttt taaaatttcc agaagacggg gttcggatac 32580
aggcgttaag cctcccagtt ccgcacacag ccgctttaga tacacggcag gaacacgtat 32640
aagcccatat tcaggatttg cgccccaatc cacaaataaa cgtataagtt caagattatc 32700
gctcttcacg gcctttacta gcgccgcttc gagacaaaga tcatcctcag aaaaacactg 32760
taaatgttta tacgaaaaaa cttgcttaca attgttacat aggtgaatag gacctaaatc 32820
ccaccacaaa ccaaaacgct gcaacgtata atcatagtca cttgaaagat aattgcatgc 32880
cacaactttt ttggccaacg tttgtaaaga caacatacta agtttaaaac atcttaaatc 32940
taagctagct aactttcaag aaaaccctct atccctaaga atatatctta taactagact 33000
tatagcagta aaaatcaact ttggttattc tttttaatat aaaacgtcta attacttgca 33060
aaggactata aagcccattt tcctcagcta gaatttttat tttttaatga agtaggggga 33120
tatgttttcc cttcaagacc tttgccgaaa gcatcttttt attcttcccg atgtttttgg 33180
cgagcatgta ctacaacgat taggactgta ttggagatgt cacggctccc ttcaacgcat 33240
aggagacgac cacatactca tacgacggga tctcatcctt tccaccaacg aggccttaag 33300
aatggcggga gaggaaggaa acaatgaagt agtaaagctc ttgttactgt ggaagggaaa 33360
tcttcattac gccgtcatag gagccttgca gggtgatcaa tatgacctga tccataagta 33420
tgaaaaccaa atcggcgact ttcattttat cttaccattg attcaagacg cgaatacgtt 33480
tgaaaaatgc cacgctttag aacgtttttg tggtgtttca tgtctgctaa aacatgctac 33540
aaaatacaac atgctcccta ttctccaaaa ataccaagaa gagctgtcta tgagagcgta 33600
tcttcacgaa accctatttg aactagcatg cctatggcag aggtatgatg tccttaaatg 33660
gatagagcaa accatacatg tttacgacct aaagattatg tttaatattg ccatctccaa 33720
gagggatctg actatgtact ccttaggata tattttcctt tttgatagag ggaacaccga 33780
agctacgttg ctaacgcaac atctcaagaa gacagcggcc aaagggctcc tccactttgt 33840
gctagaaacg ttaaaatacg gcggcaacat agataccgtc ctgacccaag ccgtaaagta 33900
caatcataga aaacttttag attattttct gcgtcaacta cctcgtaaac atattgaaaa 33960
acttttgttg ctggccgtgc aggaaaaggc ttctaaaaaa acattgaact tactgttgtc 34020
acatttaaac tactccgtga aacgcatcaa aaaactaccg cgctatgtga tagagtacga 34080
gtccaccttg gtgataaaga ttttattaaa aaaaagagtg aacctgatag atgccatgtt 34140
ggaaaagatg gtaagatatt tttctgcgac gaaagtgagg acgatcatgg atgagctttc 34200
gattagtccg gaaagagtca ttaagatggc tatacagaaa atgagaacgg atatcgtaat 34260
ccatacttct tatgtttggg aggatgatct agaacgtctt actcgtctta aaaatatggt 34320
atacaccata aagtacgaac atgggaaaaa aatgttaatt aaagtcatgc acggcatata 34380
caaaaactta ttatacggcg aaagggaaaa agtcatgttt tatttagcca agctctatgt 34440
tgctcaaaac gcggccaccc aattcagaga catttgtaag gactgttaca aactggatgt 34500
ggcacggttt aaaccgcggt ttaagcaact aatattagac tgtttagaaa ttattactaa 34560
aaaatcttgc tatagtatcc tggaaatctt agaaaaacat attatttccc tgtttactat 34620
gaaagttatg actgaagaag aaaaaaacct atgtttagaa atattatata aagtaattca 34680
ttataaaaca atacaatgtt aaaattcaat agatatccat cattaatatt gattatattt 34740
tcgaatatta tcttctatgg tgcaagataa tcatctagcg cgtgaaacat gtcctcttct 34800
cttcaggaac tttgtcgaaa aaagctgcct gactgcatac ttccagagtt ttttgacgac 34860
tatgtattgc aactgttagg actgcactgg caagatcatg gttcccttca gcgtatcgag 34920
aagaaccaga tacttgttca acaggaaccc atccatatca atgaagcact caaagtagca 34980
gcatcggaag ggaactatga aatcgtagag ctgttgttgt catgggaggc agatccccgc 35040
tacgccgtcg taggagccct agaaagcaaa tactatgacc tggtttacaa atactatgac 35100
caagttaaag actgccatga tatcttgccg ctgattcaaa atccggaaac attcgaaaga 35160
tgtcatgagt taaacagcac ctgttcactg aaatgcttat tcaagcatgc tgtgataaat 35220
gacatgctgc cgattcttca aaaatataca gactatctgg ataggtggga gtattgcagc 35280
cagatgctgt tcgaactggc atgtagtaaa aaaaaatatg agatggttgt gtggatagag 35340
ggagttctag gcgtcggcaa agttacatct cttttcacca ttgcgattag caacagagac 35400
ctacagctgt attctctggg ctactcaatt atccttgaga atttgtactc ctgtggacag 35460
gaccccaagt ttttactaaa tcatttcctg cgagacgttt caataaaagg gcttctaccc 35520
tttgtaatca aaaccataga atatggtgga agcaaggaga tagccataac tctggctaaa 35580
aaatatcagc ataaacatat tttgaaatac ttcgaaacct gggaaagcta ggttcagtat 35640
ggtgtactca ctattgtagt gaatcgtatc ctgtaaattt tgtaaaaaag cttaaacttt 35700
tgaccacatc atattgtttt agaaatctca aaccagtgaa caacagtctt atcatacatt 35760
aaaattccag taaaatttat attttttttg gtaaacaaat gttttctctt caagacatct 35820
gtcggaaaca tctttttcaa cttcctgacg cttttgatga atatatatta caagcgctag 35880
gactatactg ggaaaaacac ggatctcttc aacgaataag aaaggacgct gtgtttgtac 35940
agcgaaacat cgtcctttct accaatgagg ccctgagaat cgcagcctca gagggaaacg 36000
aaagggtaat aaaacttctg ttatcatggg agggaaattt tcattatgtg atcataggag 36060
ctctagaggg tgaccaatat gacctaattc ataagtatga tagtcaaatt aaagactacc 36120
acatgatttt atcattgatc caaaatgcaa atacctttga aaagtgtcat cagttatcca 36180
atagtaatat gtggtgtctt atacagaatg ctataaaata taatatgctc cctattctcc 36240
aaaaacacag aaatattctg acacatgagg gagagaatca ggaattgttt gagatggcat 36300
gtgaggaaca gaaatatgac atagttttat ggataggaca aaccctaatg ttaaatgagc 36360
cggagtttat ttttgatatc gccttcgaac ggatagattt ttctttatta acaatgggtt 36420
atagccttct ttttgataac aagatgagta gtatagacat tcatgatgaa gaagatctta 36480
cttcattacc aacagaacac ctcgaaaaag cagccactaa gggatgtttc ttctttatgc 36540
tagaaacttt aaaacatggt ggaaatgtaa atatggcagt cttatctaaa gctgttgagt 36600
ataatcatag aaaaatttta gaccatttta ttcggcggca aaaatgttta tcacgtgaag 36660
agattgaaaa cctattatta accgccataa ccaattgtgc atccataaaa acgttaaact 36720
tactcttgtc ttacctaaac tattccgtaa aaaatatcat tggaaaaata gtacaacatg 36780
tcataaaaga tggtgattat accatcatat tacttttaaa aaaaaagaaa ataaacctag 36840
tggaacctgt tttaacaggt tttatagatt attactatag ctattgtttt ataaaacatt 36900
ttatccaaga gtttgctatt cgtccggaaa aactgattaa aatggccgcg cgaaaaggta 36960
aactaaatat gattatcgaa ttccttaacg aaaaatatgt tcataaagat gatcttggaa 37020
ctatatttaa atatctcaaa accctagtat gtaccatgaa acataaaaaa ggaaaagaga 37080
cattaattgt tcttattcat aaaatatatc aagatattca tctggagact aaagaaaaat 37140
ttaaattatt aagattttat gtcatgcatg atgcaactat ccaatttcta tctatgtgca 37200
aagactgttt taatttagcc ggttttaaac catttgtttt agaatgtttg gatattgcta 37260
ttaaaaaaaa ttaccctgat atgatacaat atatagaaat tctatcgaaa tctgagtaaa 37320
atttattttt ttgatcagag taagaaaatg ttctccctcc aggagatctg tcgaaagaac 37380
atctactttc tacctgactg gctcggtgag catgtgattc agcgactagg tctgtactgg 37440
gaaaaacatg gttctcttca gcgaatcgga gacaactatg tacttataca acaggacctc 37500
atcatcccca tcaatgaagc cctaagaatg gcaggggagg aggggaatga tgaggtggta 37560
caactcctat tactatggga gggaaacatt cattatgcca tcataggagc tttggagagt 37620
gaccattata gcctaatacg taagctctat gaccaaatcg aagactgtca cgacatcctt 37680
cccttgattc aagacccaaa actctttgaa aaatgccatg aattagataa atcttgtaac 37740
attttatgtc tcgtattaca cgccgtaaaa aacgatatgc tttgcattct tcaagagtat 37800
aaaatgcatc taagtggaga ggatattcaa gtggtgtttg aaacagcatg ccgttcacaa 37860
aaaaacgata ttgtgtcatg gatgggacaa aatattgcaa tatacaaccc cgaagttatt 37920
tttgatattg cctttgataa gatgaatgtg tccttattat ctatagggta tacgcttctt 37980
ttcaatcatc atataaataa tacgaacgaa aatattaatt ctttattgac acaacatctt 38040
gaatgggctg ccggcatggg ccttcttcat tttatgctgg aaactttaaa gtatggcggg 38100
gatgtaacga taatagtctt gtctgaggcc gtaaaatatg accacagaaa gattttagat 38160
tattttctcc gtcgaaaaaa cttgtaccaa gaagatcttg aagaactatt attgttggcg 38220
atacgtgcag attgttctaa aaagacctta aacttgttat tatcttactt aaactattcc 38280
ataaacaata tccgtaaaaa aatattacaa tgtgtaaaag aatatgaaac gaccgttatt 38340
ataaaaattt tacggaaaag aaagataaat ctgatagagc ccattttggc agactttata 38400
ggatatcata gctataccta tatggtagat tttatgcgtg agttttccat ccatccggaa 38460
aaaatgatca aaatggctgc acgagaatcg agggaggact tgatcataaa attttccaaa 38520
aaagtttgca aagagcctaa agatagactt cactatctca aaagcttagt gtatactatg 38580
cgacataaag aaggcaaaca actgttaatt tatacaatcc ataacttata caaagcttgt 38640
catctagaga gtaaagaaat gtttaatttg gcacgatttt atgcacggca taatgcagtg 38700
atccagttca aatcgatttg ccacgatctc tccaagctca atattaatat caaaaacttg 38760
ttgttagaat gtttaggtat tgctattaaa aaaaattact ttcaacttat caaaacaata 38820
gaaacggata tgcgttatga gtaacatttt tagatgaggg aagattctac caaactaact 38880
aagacctttc gctagaatgt atcttattgt taatatagat gagatatgtc attgtgaaaa 38940
aatagattag gtaggttgtg aaaaacagat taaacttaaa attatgtgta ttatgtaaaa 39000
ttttagaaat aaaaatttat ttttttttat tgagggtacg gaaaatgttc tccctacagg 39060
acctctgtcg gaagaacatt ttcttccttc caaatgattt tagcaagcat accctacaat 39120
ggctgggatt atattggaaa gagcatggat ccgtccatcg agcagaaaaa gacagcataa 39180
tgatacagaa tgaattggtt ctttctatca atgatgcttt acagcttgca ggagaggagg 39240
gggacacaga tgtagtacag ctcttgttat tatgggaggg aaatctgcat tatgccatca 39300
taggagcctt gaagactgaa aaatataacc taatatgtga gtatcatagc caaattcagg 39360
actggcatat tctcctaccc atgattcaag atccagaaac attcgaaaaa tgtcatgatt 39420
taagccttgg atgtgacttt atttgccttc tccaacatgc tgtaaaatac aacatgcttt 39480
ctattcttgt caaatataag gaggatctac taaatgcaag gattaggcat cgtatccaat 39540
ccctgtttgt tttggcatgc gaaaatcgga gaattgaaat tattgattgg ataggccaaa 39600
atctgccaat tcctgaacct gatgccattt ttagcattgc tgttgctaca agagatttag 39660
aactgttttc cttagggtac aagattattt ttgattacat gcaaagacag ggaatcattc 39720
aattaaccaa tggagttcgc atggttgtgc taaatcgtca cattagcatg gcaatagata 39780
atggtctttt accttttgtt ctggaaactt taaaacatgg tgggaatata catagagcct 39840
tatcttatgc agtaacacac aatagaagaa aaattctgga ttatcttatt cgccagaaaa 39900
atatagcccc taatacaatt gaaagacttt tatatctggc cgtgaaaaat caatcttcca 39960
ggaaaacttt gaacttgttg ctatcttaca taaattacaa ggtgaaaaat gttaaaaagc 40020
tggtagagca tgtagtaaat gagaaatcca ctcttgtgtt aaaaatttta ttagaaaaaa 40080
aggaaaatct agtggatgct gttttaacaa gacttgtaaa acattctaca tatttccagg 40140
tgagagaatt tatccaggag ttttccatca gcccagaaaa attcattaaa atagctgtgc 40200
gggaaaagaa aaatgtgtta atcgaggcta tttctgaaga tatttgggaa aatcccacag 40260
aaagaattac ttatctcaaa cagatagtgc acaccataaa atatgaaagt ggaaggcgat 40320
ttttggtaga catcattcac agcatttacc aaagttactc actaaaacac gaagatattc 40380
ttaaactggc aacattttat gtcaaacaca atgcaatcac ccattttaaa gacctctgca 40440
aatatctttg gctgaacaga ggaacagaaa gtaagaaact gtttttagag tgtttagaaa 40500
ttgctgatga gaaggagttt cctgatatta aaagtattgt gagtgaatat attaactact 40560
tgtttactgc aggagctatt accaaggaag aaatcatgca agcctatgat gctttagagt 40620
agccatgtat taacattctg aaagtagaat aaaatatact atatactaaa aaccaaatta 40680
gccattttta actatcttct tcttaaaaac tctggataaa aatttatttt tttttaattt 40740
gggtagggaa aatgttctcc cttcaggacc tctgtcggaa gaacaccttc ttccttccaa 40800
gtgattttag caagcatacc ctgcatttgc tggggttata ctggaagggg catggatcta 40860
tccaaaggat aaagaatgat ggtgtgctta tagagcatga tcttactctt tccatcaatg 40920
aagccttaat tcttgcagga gaagagggaa acaatgaagt agtaaagctc ttgttactat 40980
gggaaggaaa tcttcattat gccatcatag gagctttgag gactgagaac tataacctag 41040
tatgtgagta ccatagtcaa attcaggact ggcatgttct cctccctttg attcaagatc 41100
cagaaacatt cgaaaaatgt catgatttaa gccttgaatg tgatctttca tgccttctcc 41160
aacatgctgt aaaatataac atgctttcga ttcttgttaa atataaagag gatctactaa 41220
atgtactatt taggcaacaa attcaaggac tatttatttt agcatgtgaa aatcggaagc 41280
ttgagattct tacgtggatg ggtcaaaatc tgccaattcc tgatcctgag cctattttta 41340
gcattgctgt tgtcacaaaa gatttagaaa tgttttcctt agggtacaag attgtttttg 41400
aatacatgga aaaccaagga cttcatttaa cccaggtagt tcgtatggtt atgctaaatc 41460
atcactttgg catggtaata aataaaggac ttttaccctt tgtgctggaa attttaaatt 41520
atggtgggaa tgtaaataga gccttatctt atgctgtcac acaaaataaa agaaagattt 41580
tagaccatgt tgttcgccaa aagaatatac cccataaaac cattgaaaga atgttgcatc 41640
tggctgtaaa aaagcatgct cccaggaaaa ctctgaactt gttactatct tacataaatt 41700
acaaggtgaa aaatgttaaa aagttgttag aacatgtagt gaaatacaac tctactcttg 41760
tgataagact cttgttagaa aaaaagaaaa acctgctgga tgctactttg acaagatatg 41820
tcaaagattc tacatacttt caggtgaaag aatttatgca agacttctcc atcagcccag 41880
aaaaattcat taaaatagct gtgcgggaaa agagaaatgt gttgatcaag ggtatttctg 41940
aagatatttg ggaaaatccc gcggaaagaa tcaggaatct taagcagata gtgtgtacca 42000
taaaatatga aagtggaaga caattcctga taaatatcat tcacaccatt taccagagtt 42060
attctttgaa acctgaagaa attcttaaat tggcaacatt ttatgtcaaa cacaatgcaa 42120
ccacccattt taaagatctc tgcaaatatc tttggctgaa cagaagaaca gaaagtaaga 42180
aactgttttt agagtgcttg gaaattgctg ataagaagga gtttcctgat attaaaagta 42240
ttgtgagtga atacattaac tatttgttta ctgcaggagc tattaccaag gaagaaatca 42300
tgcaagccta tgctttggag tatgccatgt attaaatttc tgaatcagta agcaatagat 42360
agattttaga atatgctgta ttaagttagt ttctgaataa gtaattaata gatagatttt 42420
agtttatgta aaaatgttaa catttgttca taagttttag ataccatttt agagttactt 42480
ttttagatat tactatttta gccattatta tcttaaataa tcactatttt agataggtcc 42540
ccgtattaaa aaccaaatta accattatct atgtttttaa taatactttt taaaaaccct 42600
ccataaaaat ttattttttt ttcataaaag tagagaaaat gttctcccta caggatctct 42660
gtcggaagaa cctttttctt ccacttgagc ccttaggcaa gcatgtggtt caacggctgg 42720
gattatactg ggaaggccat ggttcagtta aacgagtggg tgattgcttt atatgtgtag 42780
accagatttg gatgctatca atccataagg ctatacaaat tgcagcctcg gaaggaaatg 42840
agaacattgt caagcttttc ttactatgga aggggagtct acaatatgcc atcataggag 42900
ccttagaggg caggcaatat gatctgattc aaaaatatta caaccaaatt ggggactgcc 42960
atcagattct accactgatt caagatccag aaatttacga aagatgtcat gaattaaatg 43020
ttacatgtac ctttcaatgc ttatttcaac atgctataag agataacatg ctgcccattt 43080
tccaaaaata tggagaagat ctgaatggaa acaggagaat ggttcaactt ctgtatgaga 43140
tggcatgccg attacaaaat tatgatatca tcaaatggat aggatctaac ctgcatgttt 43200
ataacttgga agccattttt agcattgctt ttgttagaaa ggatttaact ttgtattctt 43260
taggctacat gcttcttctg ggtagaatga gtactgaaga tagaaacttt atctcaatca 43320
taacacgcca tcttgaatac gcatcaaaaa agggactttt tgactttgta ctagaatctt 43380
tgaaatatgg aggtcaagtg gatacagtgt tgtttcaggc tgtaaaatac aaccatagga 43440
aaattttggc ccattttatt catgaaattc cccgtgaaac ggttgaaaag ctgatactcc 43500
atgctgtgga gtcacgggcc tccagaaaaa cattcaacct gcttttatct tccataaact 43560
actgtgtgaa cccttttgtc aaaaaactac tgcacgctgt ggtggaacac aagtacatgc 43620
ttatcataaa gcttttgctc gagcggccca aaaagaagat aaacctggta gatgctgctc 43680
tattcaaact tgtaaaatac tctacttata cagaaatagt aaaatacatg ggtgagtttt 43740
ctgtggaccc aaaaagggtg gtcaaaatgg cagcacgact catgagagtg gacctgatta 43800
aaaagatttc taatgatgca tgggaagata aactagagag aatcaagcac cttaaacaga 43860
tggtaaatac catgaaccac agaaatggaa aaaatctatt gatgtacaat attcacaata 43920
ttactggata tacctatctg aacaccaaag aagcatttaa cttaacaaga ttttatgctg 43980
tccacaatgc aacatgtttg tttaaagaaa tgtgtaaaag ctgttttgta catgataaaa 44040
tacagctcag agaattgctt gaagattgtt tacatattgc taataggcat gattatatcc 44100
agattgcaga aaccgcagat gaatgtatca aatatataga tcttattaca tttaagtaaa 44160
ccatgtatat atcaagtaaa tccagattaa atcaggctaa ttgtaaatag ttgtagatac 44220
catataatga atgttttatt aggatagtag ttcagttaag atagtagttt agttaagata 44280
gtagtttagt taagatagta gttatgttaa gatagtagtt ctgttaagat aatagtttag 44340
ttaaaactag ttcatgttaa gttaatagtt ttgttaagac aatagttcat ttaagtcaat 44400
agttcagtta agtcaatagt tttgttaagt caatagttta gttaagtcaa tagtttagtt 44460
aagtcaatag tttagttaag tcaatagtta tattaagaca ttagttctgc taatacatta 44520
gttttgttaa gataataaaa atttattttt ttttcatcag ggtagagaaa atgttctccc 44580
tacaggagct ctgccggaag aacatttaca ttcttcctta ccccttggct aagcatgtac 44640
ttcaacaact agggctgtac tggaagggac atggatctct tcaacgaatc ggagatgacc 44700
atgtactctt acagcaggac ctgatctttt ccatcaacga ggccttaaga atggcaggag 44760
aggaaggaaa caatgaagta gtaaagctct tgttactatg ggagggaaac cttcattatg 44820
ccatcatagg agctttagag ggcgaccgat atgaccttat ccataaatat tatgatcaaa 44880
ttggggactg ccacaagatt cttcctttaa tccaagaccc gcaaatcttt gaaaaatgcc 44940
atgaattgag taactcctgt aatattcgat gccttttaga acatgcagta aaacacgaca 45000
tgctttctat tcttcaaaaa cacaaggagc aaataagatt acacatggca ttaacccaaa 45060
tactatttga attggcgtgt catgaacgta aaaatgacat cattagatgg atcggttatt 45120
ccctgcacat acaccatcta gagactattt ttgatgttgc attcgcccat aaaaatttat 45180
ccttatacgt tttagggtat gaacttctca tgcacaaagt aaatacagag gctgcatata 45240
tagaattacc caatttgcta tcatatcacc ttcgaactgc ggcggcagga ggtcttctta 45300
actttatgtt agaaacaata aagcatggtg gatatctgga taaaacggtt ttatccgcgg 45360
ctatcaggta caagcatagg aaaattgtgg ctcattttat tcatcaggtt ccccgtaaaa 45420
ccgttaaaaa actgttactc tatgctgtgc aggctcgggc ccccaaaaaa acactgaacc 45480
tacttttatc ttccttaaac tactccgtgc acaccatcac caaacaactc gtacacaatg 45540
tcgtcatcta cagttccacg cttatcgtaa agcttttact catgcggcga aaaaacaagt 45600
taaacctagt agatgccgtt ttagccagac ttgtaaaata ttccacctat acagacattg 45660
tacaattcat gggtgagttt tctgtgagcc cagaaagggt gatcaaaatg gctgcacggg 45720
aatccaggac ctttctgatt gaaatgatct ccaaagctgc ttggggaaat cacccacaga 45780
cgttgattca tcatctcaaa caactaacca ataccatgaa gcctcaatct ggaaaagacc 45840
acatcatata taccatccac tatatttatc taaactctaa tatgctggta gcggaggagg 45900
aaaaaaatat ttttaaatta gcaaaatttt atgcgaatca taatgcggta aacaggttta 45960
aacaaatttg tgaagactat tatatattag atgcacgatt taaaacactt attttagaat 46020
gttttgaaat tgccgtccag aaaaactatc ctagaattgc aaatattgtg gatgactata 46080
ttcgattcct tttttacagg ggaaatataa ccgaggaaga aattcgtgaa gcctattctt 46140
taaaagatgc tgaggtttat gtagatttaa aatggttaca acaaggagaa atggtttaaa 46200
ccaaatccgg tttaaactaa atccaattta aactacattt ggtttatcat tagtcattga 46260
aaccatcgaa aaaaaagcta tttgtttatc cccataaact catctttttt ttgtctcaaa 46320
gtttgacact aaaattcagt gttttatagt gtttataatt aagtgttttg catgcattgc 46380
agaaattttc atctttttta attggttcaa taccacatgt catacaatat gttgtttgat 46440
tatcaagatt aactttatga aaggaaagta agtgagccgc aaatttaaaa gtaaaatatc 46500
tttcatttaa aatgatctta tgaatgtatt ttcgataagg aggaatgaaa gcatttgcca 46560
aaataaatcg cataaaaggc ttggaaaaac ccatatcttc taatcttttg tgggtataaa 46620
ccctattttg gtgttttaca aaaacttcat tgttataata gtcgttatag ctatcaatca 46680
tttttttaag tcctataatg cccaaggttg cacgcataaa gccacagttt ctgctccaaa 46740
aagcatgcac ctgtaaaggg tgcttttcat ataaccaatt acaaaatttc attccgcaac 46800
agtagcatgt tatttcagtg ggggatgtat agaataatcc ggcattcgaa aatttttcat 46860
aattttttat gtcatggatt gcgaagcttt gatttcgtgc atctatggag ctatagccta 46920
catatttagg ttttacttca aataatcgca aagagatgta tggatctatc gtatttattt 46980
taggaaacat ttcataattt taaattctta tatataatat aaaaaaaatt acaaacattt 47040
gtaatgatca tcctcaattg aaggctgagt tgtaggcttt atttttctaa ttatacgaag 47100
aaggtaggtt ctcataaagc cttcaagatg actattgatg tttccaatac attttctcaa 47160
tgagttcata aacccagaca ttttgctaat ggcttggcaa agtgccaaca agttgtccac 47220
aaagtactgg tagattgcca ctagctatag ctagctatag tgagccaacc tctctgtatg 47280
tattttatat atttcatttt ttaatagatt taatattttt ataaaaaaat atttagtttt 47340
ttatacaaga atgtcgacaa aaaaaaagcc cacaattacc aagcaagagc tttactcctt 47400
agtagcggca gatacccagt taaataaagc attgattgaa agaatcttta caagtcagca 47460
aaaaataata caaaatgctt taaagcacaa tcaagaagtt attataccac ccggaatcaa 47520
gttcaccgtc gttacggtga aagctaaacc tgctcgccag ggccataatc ccgccacagg 47580
agagcctatt caaattaaag ctaaacctga acataaagcc gtaaagatac gagcattgaa 47640
acctgtccat gatatgttaa actaaactat aaagtcatat tcttctttat cgttattatc 47700
ttcaatatat ttttgccaat cgaaatcgaa taaattcaga tcctggacat ttaaatactt 47760
atcatcgtac attttaatat aatttaaaca tgagttgttg tcaaaaactt ttagcgtttt 47820
tgttaaaatt atcatatgaa taatttcctt attaagagtt gccggaataa tacaaaacct 47880
atttttaggt acatcatcca tgataatagt aaaattagta aaaattgttt cttgtttttc 47940
ttttgtttca aataaacgtt gtaaggttaa aggtttctcg ttcaatggtt tctttgaaga 48000
taaaaagaat gtataatctg gtttaaaggt atttttggtt tcaatcgtga ttccatctgc 48060
ttgagcatat actaaaccag accaaatata acggtccact attacaatat aatttagctt 48120
aagtagcact gcaatttctg cgataaattc actacgatgt tttgtaaata atttatgtaa 48180
ttgttccgat gacatttcta tggttttatt taacacctgc aatataagat caccggtggt 48240
cgtgtctgga ttaggaaaat gtatacatat agcattataa tccatgcatt ccaatgtttc 48300
ttttaatttc attgcctgtg tgctttttcc cacaccattg attccctcga tggcaatgag 48360
tattccacgc atgattaata aaaggaaaaa aagaattcag tttttaacat ttcttacaaa 48420
tcttttttta tacaacattg tacaacactg cattagcggt atatgatgtt atagcttcat 48480
taaatatttg cttttatata atctttacca acctatattt ggtagatcac tgcagatggt 48540
cataaatagg ccataactaa gataaaaatt atttcagacg ctactacggt agtattatta 48600
aaatcatgtg tggcaatgta tgacgtctta atagataaaa catttaagga aaacaaattt 48660
gaataaaaaa aataattgtt atgatggcgt tgttacacaa agaaaagctt atagagtgca 48720
tctatcatga gctagaaaat ggcgggacaa tattgcttct aacaaaaaat attgttgtgt 48780
cagaaatttc atacattggc aatacttata aatattttac ctttaatgac aatcatgatc 48840
tgataagcaa agaagatctt aaaggagcaa catccaaaaa cattgctaaa atgatttata 48900
attggattat aaaaaatcct caaaataata agatttggag tggtgagccg cgtactcaaa 48960
tttattttga aaatgattta tatcatacaa attacaatca taaatgtata aaagattttt 49020
ggaatgtttc aacttcagtc ggtcctcata tctttaatga tcgtagcatt tggtgtacta 49080
aatgcacatc cttttaccca tttaccaaca ttatgtcgcc caatatattc caataaatta 49140
gatatctttg ctattaaaat agttaaaaac cttataggat aattaggtac tttattacga 49200
taaattatga tattttataa ttagttactt tattataatt aatctcttta ttaatgaatt 49260
atcataagat aactaattat ttttttccat atatcagata ataaatctga tatgggctaa 49320
aagtatgttt caaactattt acaatagaat ttctgttaag aaaacataca taatttgaat 49380
aaaatttttt taaatatcac cgaaacaatc aacatggtgt taatagagtt tttaacaggt 49440
ttcttctatt tatatggaaa gagactgttt tccattagta aagtcatgga catgatatgt 49500
ctagactatt ataccattat tcctgctcct ctggcgatga tgttagcggc aagactaaaa 49560
aactatgacc tcatgaaacg actgcacgaa tgggaaatct ctattgacta cgctctactt 49620
gtagtagatg atgtgccgtc tattgactat tgcttaagtc ttggcgctag atccccgact 49680
agagcacaaa aaagagaact gctgagggac aacacgttta atcccgtgta taagtatctt 49740
atgaactgtt ccggcttccc aacaaagaga gaaaaaaaca ttccttgtga tgttcaatgc 49800
gaaagactgc aaaaaaacat tataaaagaa ctggtattta actgctctgt actgcttgaa 49860
atggtactgc acacagaaag agaatatgca tacgccctac actgtgctgc aaaacataac 49920
caattgccca tcctcatgta ttgttggcaa caatccacag acgcggaatc tattttgttg 49980
aaaacctgct gttctgataa gaacatcaat tgttttaact attgtattct atatggcggc 50040
gcccaaaatt tggatgctgc aatggtggaa gcggcaaagc acgatgcccg gatgctgata 50100
aactactgtg tcatgcttgg tggaagatcc ttaaacgaag caaaagaaac ggctgccatg 50160
tttggacaca ttgaatgcgc acaacactgt tttaaactgc agtcttacgt cgtggacaca 50220
tcgaatacag acgacactga ttaaagcgac aatcttacgt catgaacgac tgtcttttga 50280
gtatctatac ttacattata tttttttatg aaaaaaatat aaaggttgta tacaaacctt 50340
tgtatacaag aaatttggat cattaaacaa taattaattt ggacacagga aacgatctag 50400
atcgatcaaa aagctatttt ttttgcacac agaacattta gataattgag agattacttt 50460
ccatacttgt taagcttttt tacacacagg aactttggat tctgttcagg aagtttttca 50520
tagacattat gtttacagcc agtaataata attttgggct ttttcttaaa ccaccggtgg 50580
aaaacatcca gcttgtaaag agggaaatgc atgtagagag gttttggtag tcatggttaa 50640
gagatttgac taactccatg tttcctgtaa agactgccca gtcccaagca gtaaaacctc 50700
tatgatagtc tttttgagtc ggatctgctc caaattttat gagagaaagc atatttaaag 50760
aacggccccg tattgcggcc ttcatcacag gagtcatccc attaaaattc ggtaaacaaa 50820
ttctggtccc attttttccg aaatagccca acaccccttc caggattaaa tgattttttt 50880
tctcagctaa ataatgtaaa gcagagtttc catctttatc cctcctatga gggttaatta 50940
tttctccagg ataagattct tgttcaaaaa gaaattttaa aaagtctata cgtccgtaga 51000
tgcatatcca catgaatacc gaggatccat ttttatcgca tctattgaca atccacggat 51060
ctgttttaaa aaattcctca aatagtgtaa gattcccatt tctaatatgt tttttaatcc 51120
atttaacaaa caagttttct atctcccttt ctggaaacat gtgttccatt ttgaatgtcg 51180
cccctactcc actatatgat tttactcctt taatttttaa tgtccttttt tttcggactt 51240
ctttggataa gctgtttatt accatcttta aatgccttat agcggggagg agccaggccc 51300
ttttcccata tgtgcggtaa ttcttggtgt ttatgcttgc ctttggcata accaggccag 51360
tatttttcga tatattcagg gtttgttttt acgtattctt taaaggtccg ataggcttct 51420
tgaatacagg taggctcacc ggtataattt ccatgttcat cttcctttaa aaagccatta 51480
accctgtcct ttctccactt aagattgtgc tttccaaaaa tgcgatcaag atcttgcgcc 51540
tgctggggtg gaatcataaa tcccttttta ggtcgaagct ttttattttt tccatagctt 51600
cggccatcgc gttgcgaaac agtggttagg acgcctgata gtctttccat gggcgtcgca 51660
tctaatccta tccatccacc ctgatgaata tcaatggcaa caagctctcc tttattttgg 51720
gcaagccaag tttccaagaa tgccatgctt tcttcccagg gataaggccc gccaacacca 51780
cgggttgtcc aatcttgcaa ggactccagg tccgacacct ggtaaggctc taaagaagac 51840
ggttccttgt ttttgtactg caaataagat ttaatgaccc atttatacca tgtgtcgaac 51900
cgcagcgtgg cgcctccaaa gtgaaagccg tcgttgattt taggatatct gcaacatatt 51960
tcaaccgtac gtttgagttc tgcaaaagcg gccttccaag gaagtctttc gctgcgggta 52020
agacggtcta ttttgccctg cgtgccatag cgtatggcat gtcgtgccaa ttgcaacaat 52080
tctgacaccg atccgtgggc cccgatccag tttatcggat aggcaacctc cgaagggttt 52140
aaaagatgct cgtaaaagcg tggatcttca gatgccaagg cgtctgcaaa ggggataatg 52200
ctagaaaacc tgtctagaca tacgttttct gtgtttactt ctaaaggtag aaaaatggtt 52260
gcgtgaggct tttgaacctg cttgttcagc ggtctgcata tgctttgaat aatgtctcta 52320
ggactatgtc gcggcgctgc aaaaaatacc gcgtttagtt ctggaacctc tacgccctct 52380
tgaaagagtc gacagtttaa taaaataacg ggttcctttg aggaacaaaa ttctgtaaat 52440
gttttgagga taacctgtcg cggcagggtt gagtgagcta tcagggcata gaccccttgg 52500
tctaccaacg ccgcgtatag ctccttggcc tgtttaatat cacgggtaaa taccagcatt 52560
ttaggagccg gtatattggt ttttaaatag gctaaggcca ttataatttg ctttactatg 52620
atctgtttcg tggtctcctc tttggtactc ggttggtggg ccaatttagg cgcggctacc 52680
atctgcaatt caaaatcatt tacatagccg gcctctatgc cttctcgcag atagtagcga 52740
aaggcaacgc cgccaaaaag ttcacgattt ttcatggaaa gcggggtgtc gtacctgggc 52800
gttgccgtta aaaaaagtcg gtgccctttt ttaaagttga gcaacacgtg ggtaaagggc 52860
cgtgtctccc attcgccgca aatccggtga cattcatcgc taataataag atcgaaatca 52920
tccaccagta gcgtggagga ttggtaggtg gcaatcacaa gaagagaagg ggcctcccgt 52980
atccgttttg caataaagac aggattggtg gtcatttcta tattgtcgtg atttagcaca 53040
atgcgggtct ggtcagaccc cacaagcaaa acgttcttca aagaaattcc atactgatag 53100
agtttttcca gagtctgccg tagtagggac aggcccggca ccaggtacaa aacttttcct 53160
tgaagataat tggagaggat aagataggcg acgcgagttt tgccgcatcg gcaggccatc 53220
tgcagaatgg ccctcccact tcgccgcagc tcctgatagc ccatattggc cgcctccttc 53280
tgataaagtc gatcctcgat tgcagtccgt gtctcatctg tagaaaaaaa taatacgtca 53340
tctgcgaaat gttcatcttc cacaggagtt atcaccaggt gtctcagttt ctccttgctt 53400
atcagcggat cagagggcaa agatggctca accactatcg tggaatcatt catctcatag 53460
gcgggagaat cacacaaagt atagcttatg tccagacagt ttgcaacatc ctcagccaat 53520
tgttttattt tttcgggtaa aagacatacg agttctttgt ttttgacgcg aaaaaactgt 53580
gcacaatata acacccctgc ttcaattttt tgcgcatcct tctttgtaga tgtttccaat 53640
gtgaaacaat acttccattc atccgtaaaa caggttgtat aagatccatc atgaagccta 53700
gcggccaagt ttcctgtgtg cccaacttta tgtaaggatt gggcctccag ccagggatga 53760
accgccacgt aaaatcctgc gcacatgcta tatcaaattg cagtttctta ataactgtac 53820
acaggatctg aaaaacatgt gattacaaaa tttagataag aaatatttaa tattaaaaat 53880
cacagaatac atgtcactgt gtagagagaa agccaaaaac tcctcttgac cgccgtggga 53940
aatcatccag ggtagtaggt tgtgtttcat aaagttgtat gccgtagtga tcaccgtgga 54000
ctccagatgg ttattggcat ctttgcaata ctttgccatc ttggcagaaa agacgataaa 54060
tccacaaatt ctaccccagt tgataagatc cttaaacagc tcagtcacaa ccccagtaaa 54120
ctgggtttta atttcttgaa cactcgtaag agaaaaggta attgtaacct gtttgttcaa 54180
acactcatca taataggtta aaattttttt tatttgttgt tgatatgggc taagctcatg 54240
ctctgaaata tcattaatgt aatatttaat atatcccact agtatttcat taatgatatt 54300
atgatatatt aactcttctc cctccatagc ggcaccctat atttttttat ttaggtttca 54360
atgttatcac aattgcgata caattgtgat acaattgtga cacaactgtg ttgtatacaa 54420
caaatgttag gccacgtata gcaacctata tgttaagaaa tatttttatc ccaacattag 54480
ttggaaacga gcagccgcaa agaagtcatt taaaataagc catttaaaga tttagaattt 54540
atatgtatac aactgtacaa tggaagcagt tcttaccaaa ctcgaccagg aggaaaaaaa 54600
ggctctccaa aattttcatc gttgtgcttg ggaagaaact aaaaatatta taaacgattt 54660
tcttgaaatc cctgaggaac gatgcaccta taaattcaac tcatacacaa aaaaaatgga 54720
gcttttattt acccctgaat tccacaccgc ctggcatgaa gttcctgagt gcagagagtt 54780
catattaaac tttttgagac tcatttcggg acatcgagtg gtattaaaag gccctacatt 54840
tgtttttaca aaagagatca agaatctggg cattcctagt accatcaatg ttgactttca 54900
ggccaacatt gaaaatatgg atgatctaca gaagggaaat ctcatcggca agatgaatat 54960
caaagaaggc taaataaaac aactaacatc aaaaaacatt aaaggctatg ttgtggacga 55020
tgcctttgtc tcaatagttt cgaggtcatc caataactca tgtaacgtaa aaaagttggt 55080
ccattttttt gaaaacatta aaagacgttc gtcttcataa ataaaaaagt cattcgaagg 55140
aaaaatgata tactcaatac catagtcttg taatattttt tttaggtctc tcagggtcca 55200
gggatttacc aggcttctac gcgaagtgag catcataaaa atatctaata ttttttgcgc 55260
cataagccag cgcggattct cattggccca caaatcaaca ataattctct tatcaaccgt 55320
gagcattcct acttgattcg aagaaatgat tagatgccca gcagtccacc ccatgagtag 55380
ataacgcagc gttgtagaaa tgtcacatat ggaaggcatt cctccacaac atgaacccaa 55440
attaggatgc gtgtgaaaca caaacatagc aggcttgttg gccaccctgc tataaatatc 55500
agcaggcatc atagcctcgc tgccaaaata aatgttctct cctgccctat aggggcttgg 55560
aatgatttcc actatctcgg gtacaccgtt tatcatatta atgcggccgc accattcacg 55620
gtcatcgtcc aaaaattttt tgatggcacc ccgaacattg tcccagttaa gcaacagagt 55680
attcacaatc tcattacgct ccgcccagta ttccttaaaa cttcttttag acttgctgag 55740
ctgttcccag gattcgaact cagtccaatg ttttttttct tttggggaag acttcccttt 55800
tgaaacattt tttgcggctc caccatctac actatgattt tccaaaataa tctccttcat 55860
cgtttgagtt atatgggcat tgctaagcac cttagtggta acctgtttac ctatgtgatt 55920
tagcagaaaa ccaagtttgt ccatttgtgt ctcaaccatt tattcttaac aaaacaaaaa 55980
aaaattaaaa atcatcgtcg tttaaaaaga gtttgaaggc aaacgcatca tccttaacac 56040
agttctgata ctgcgtaggt cttaactcga aaaagttggt tttttctact tcattaagaa 56100
agaatttagt catctgagga aaagggtttc ccaccttata aatgcttttg cactgcatca 56160
tgaagcacaa attatctgta aagtagcgta tatattgaaa tagcatttct tttgaaaaac 56220
cgggaactct tcctcttgcc ttgtcaaagg catagttaat aaactcatcc accaactcca 56280
cagcctcctt caaaattttg tgaatgatct tttcctcggg aatgttatac acgtaatttg 56340
agataagaaa acacgcaaaa ctacagtgca tcccttcatc acgtgagata aactcattat 56400
agcttacaag ccccggcata atattctgtt ccttaagaaa ctggatcgcc acaaagtggt 56460
tttgaaataa aatgccttct acggcggcga agcccaccag ccgctcacct agagtgttcc 56520
tgtcggggtc catccactgc cgcacccact gcgccatttt ttttatgata gggtgttttt 56580
caatgccgct aaagatgcgc tgttgttcct tctcatccgg gatcagcgtt tttacctgta 56640
ttgagtaggc ttcgctatga acgcactctt gggcagcctg cattgtataa aagtataaca 56700
cttcctttac tttaatttcg cgcataaaat tggttaaaag gttttcgata acaatttcgt 56760
cggcaacaac aaagaaggct aaaatttgtt tataaaattc gcgctgtggc tttggcatgg 56820
cttcccaatc atcaatgtcc ttacacatgt ccacctcctg cgccgtccac gtcaaacttt 56880
ctaatttttt ataccagttc caacattcgg ggtgctgaat aggaaaaata gtgaaacgtt 56940
gggaattttc aattagtaat tcctccatat ttgaaataaa tattaacatc ttcaaattta 57000
ttggctgcca tggagacgtt ttttattgag acgttggcat ctgatgtgta tggaaaggcg 57060
ttaaatgttg atttagatag actatcgcag gcgcaggtta aatataccct tcaagagctt 57120
atttcctact gcagcgctct aaccatttta cattatgact attcaaccct tgcggcgcgt 57180
ctttcggtgt accagctgca ccagtcaacg gcctcctcct tctcaaaggc ggtgaggctg 57240
caggccgcac aatcctgctc acgcctgtcc ccccagtttg tggacgtcgt ttacaagtac 57300
aaagccattt ttgacagcta cattgactat agcagagatt acaagctgtc cctcctgggg 57360
atagaaacca tgaaaaattc ttatttgtta aaaaataaag atggggtcat catggaacgc 57420
ccgcaggatg cttatatgcg ggttgccatc atgatctatg ggatgggaag agtggtcaat 57480
atgaaaatga ttctgctaac ctatgacctg ctttcccagc acgtcatcac acacgcgtcg 57540
cccaccatgt tcaatgcagg caccaaaaag ccacaactct ccagctgttt cctgctaaat 57600
gtaaatgata atttagaaaa tttatatgat atggtcaaaa cggccggcat catttcaggc 57660
ggcggcggtg gaatagggct gtgcttgtca ggaatacggg caaagaatag ttttatttct 57720
ggtagtggtc ttaaaagtaa cggcatacag aattatattg tgctgcaaaa tgcttcacaa 57780
tgctacgcga accagggagg cctacgtccc ggagcctacg ccgtctactt agagctgtgg 57840
caccaagaca tctttacatt tttacaaatg cctcgcctaa aaggacaaat ggctgaacaa 57900
cggcttaatg cccctaatct caagtacggc ctatgggtcc ccgacctatt catggaaata 57960
cttgaagacc aaatacacaa cagaggcgac ggcaaatggt acctcttttc gccggatcag 58020
gcccccaatc tacataaggt ctttgatttg gaacggtcgc agcacgaaaa cgcacaccgc 58080
gaatttaaaa agctttacta tcagtatgtt gctgaaaaaa ggtacaccgg cgtcacaacg 58140
gccaaagaga ttatcaaaga gtggttcaaa acagttgttc aagtagggaa tccctatatc 58200
gggtttaaag atgccataaa tcgtaaaagt aatctttcac atgtaggcac tatcacgaac 58260
tccaatcttt gtattgaagt cacaatcccc tgctgggagg gtgataaggc tgaacaaggt 58320
gtttgtaatc tggccgcagt aaatctagcc gcctttatac gtgaaaatgg ctacgactac 58380
cgtgggctca tagaagcatc aggcaatgtc acagaaaatt tagataatat tatagataat 58440
ggctactacc ccacagaagc cacgcggaga agcaatatgc gtcaccgacc tattggcatc 58500
ggggtctttg gcctagccga cgtgtttgcg tctttaaaaa tgaaatttgg ttcacccgag 58560
gccattgcca tggatgaggc catccatgcg gccctatact acggggccat gcgacgatcc 58620
atagaacttg caaaagaaaa aggaagtcat cccagctttc cggggtctgc ggcctcaaag 58680
ggtctactgc agcccgacct atgggttcgc tgtggtgatt tagtttcctc ctgggaagaa 58740
cgcgtggcac agacgacgca gggtgtgttg acgccgaaaa ggtggtcgca gctacgcctg 58800
gcggctatgc agggacttcg aaatggatat gtcacagctc ttatgcccac cgcaacctcc 58860
tcaaattcta caggaaaaaa cgaatgtttt gagcccttta catccaatct atatacacgt 58920
agaacgttaa gcggggagtt tattgtttta aataagtatt taatagacga tttaaaagaa 58980
attaatcttt ggacagaagc cattcaacag cagctactaa atgcgggagg tagcattcag 59040
cacattttgg atataccggc cgagatccgc gatcggtata aaacctccag ggaaatgaat 59100
caaaaaattt taacaaaaca cgcggccgca cgaaacccct ttgtatccca aagtatgtcc 59160
ttgaactatt acttttatga acctgaacta agccaggtac ttacagtgct cgtcctaggc 59220
tggaaaaaag gtttaactac cggttcctat tactgtcatt ttagccctgg agcgggtacc 59280
caaaaaaaga ttataagaaa ctctgagaaa gcgtgtaatg cggactgcga ggcgtgtctt 59340
ctgtaggtgt ctcgcggtaa aagagcagcg gggaccatat ggtaaacccc aacaagagga 59400
taatgaataa aaaaagtaaa caggcatcca ttagttccat attaaatttt tttttcttct 59460
atataatgga atattttgtt gcggtagaca atgaaacctc cttgggggtt tttacttcta 59520
tagagcaatg tgaagaaacg atgaaacaat accccggcct ccattatgtc gtttttaagt 59580
atatgtgtcc ggcggatgca gaaaatacag atgttgtata tttaataccc tcgttaacct 59640
tgcatacccc catgtttgta gaccactgtc caaatcgtac caaacaagca cgacacgtat 59700
tgaaaaaaat aaacttagtg ttcgaggaag agtctattga aaattggaag gtttcagtaa 59760
atactgtgtt cccccatgtt cacaacagat tatctgcgcc gaaactttcc atcgacgagg 59820
ctaatgaagc cgtagaaaag tttttgatac aagcaggacg actcatgtct ctgtaaatgt 59880
ctcttccttt atgggtgacg tctcttcctt tgccgaggaa gtctctgtta tgggcaagag 59940
gtttgaaaca acgcaaggac tctgcttaat ctgctgtctc acaaagggaa tcaaactacc 60000
tgctttcgta tttttaatgt agtaattacc cttgttgtga tgaattttaa gaccatagcg 60060
tagtcccagt actttattaa tgaattttaa aattgtttga gggtccgttt tattgggctt 60120
tttaagctta aactcaaagc tgatcgcgct taaatcatac tgaacaaatt catcaacgag 60180
tttcgtcatt aattgttcat tggtcaatat attagggtcc tgaacgcatt taaagccgca 60240
cttagttaat agcataatag cgtacatatg agattgaaaa ctataattaa attgtagatc 60300
atgatgctct gcgtgttgca tggcccattg atgaaagttt aattcctgag tttgtaacat 60360
agtgagcgac tcgtatactg tctttccgcg gcttatttgg acacggccag tatagttctg 60420
ttttgtcata aaactattgt attgttcaac aaatttggga gtaattttat gaccgtgcca 60480
tgcataaaat tcgagtagtt tatacttttc atacgcaaat aggtcttgct ggtctactgt 60540
gatgccttcc tttaagtttt gtttaatttg taaagcttta ttggcatcaa tggtttcagc 60600
cgaggcaatg tttacatagt cctggtgttt aatttccatt ttaatgcttg tatattgttt 60660
gactgtctcc agcttttcac ccgtcagtat aaacacctta gcgccggtgt cggcgatctg 60720
gttaataaat cgggttataa agtgattttt tgatagatgt tgtatccgca ttgtttcgag 60780
ccatagatgg tagtatggag ttttataata tatcggccta cctgtttcct tactatacgt 60840
gaaggaaagc tggtgattgc ttatggtctg aaaaagggtg tcacgttttt gtaacgtaaa 60900
catttcaatg tcttcgatgg tttctggata gtaattttgt ttcccctgta agcagatttt 60960
ataacactta ctttttaatt cacgcacgcg gcccaacatt tggcaacatg tttctacgtc 61020
acacgacata ttgttaaaaa agccgtataa aacatcaaat ctcttatctt cgtatgaaac 61080
acccgctgaa atcgtgggcg tatagataag gatatcaacg agcccccaat aatacgatac 61140
attattaaaa tgggattccc gttcatgagc agtgctttta gaactataaa acccaatttt 61200
tttttccgga aacttttttt ggataaatga ttgcaacagc cgggcctcca ttaatgaatt 61260
tgtagggata acaatttttt tgtcttctag caaatccttt aaaaggttat ttaaccaagt 61320
ttctcgtgaa gaggtaaaat aatacgtgtc atgctgggcc cttttatatt gattccagtg 61380
aaagaagata gggacatccc cgcgaaaacg ctgtagaata ttatacgttc gatttcctag 61440
gtttgcgtcc aagcatataa cataatttgc cgtttcgagc atccacatga aaatggcaaa 61500
agagggagca aagtatttgt gcaggccgct attgaattga ttaaaaatcg attctacctc 61560
atccaaaata agtaggtcta caggctcggc tgtggaggtt agccggaaaa gtgattctac 61620
ctgaatgatg actctttcgt agctgtccaa atctccagtt acttcgctgt acaatgtgaa 61680
attcggtagc cgggattgta tattttttga gaagatctgt cgaaacgtca caaaccgtat 61740
ggtttgttgt tttgaaatag aattattgcc gtagtatttt tgcaaatagt tgcgcagttg 61800
gacggtttta cctattttca tttgagcctt tacaacaagc gtagggactc gttcatattc 61860
tcgcatacta ctttcatcat agatgtgttt ttgagtatca ggcagttctt caaagagaat 61920
ggactcatga acctctatgc tctttgtcat cacttggtcc acatatgttt ccacaaaatt 61980
atttgtgccg gaaaggctgc ccatgagaag gctatgttta ttgtcatggc gacagtgttg 62040
atacactttg tttcccgtga ctcttaaaat tagggtattg tccttatcat gcatacgctt 62100
acatatttcg cagtaacttg gacttgtacg tttaaacaat actaaatttt tatgaacacg 62160
gaggaagcaa tgatttttac atagtgttcc tgcaaatttt aatacctctt caagttcact 62220
ttgttggata gtatcgcagg aactcggtgt tgtttctttt acatttgtga agatacaagg 62280
taaacacgtc gtttcaaagg gggttgctat aagggtatca ctctttttcg tggttgtact 62340
ggtctcaaac acctctgcaa gctcctcatt aaacatttta acacgcatgc tacctttttt 62400
atgagaccct atgatgcgaa aattttgaat acttttgttg acctgggggt caacaaaagg 62460
ataaacgtgt ttgggaagat tttctaacac tttggatgta aagactttgg cctcattatt 62520
gtttaatact gagtatgtat aaagtatgat atgaaaggag tatttaagtt ctcgcttttt 62580
atttaatccg atagaatctg ttagcaaaat ttgttcacgc gttagattga tgttataagg 62640
taaagaatat gtctcgtaaa atacatccat gatgacgtta attatcatgt caaggatgtc 62700
atagacattg tcttcgacat tatcattgtc atcaacattg tcatcagagt atgacttatt 62760
taccggaaag tcgatgtcaa attttaagcg ctgaggcaaa aacccaaata ccacttcgtg 62820
gaaacacttc tgctcaaagg gctgagccgc ctcccactcc caaaagtcat cacgacttga 62880
aaaaactcta aaaagattat tatattcatc tcgcaccacg aagtgattct ttaaggtttc 62940
gagagaatat ttatcctcta cggcttctcc ttgggagtta cagcgaagaa acttgaatgt 63000
ttcttgcatt ttgatattta aaattaaatc aattatgatg cggccgctaa tgcggcggtt 63060
gacgcggccg cgccgctgac gcagccatca tacataaagc ggcatggccg ttttataacg 63120
actagtcggc cgttatatga cgaactatat aaaaatgaat tcttttaatt agagttaagt 63180
attgttgatt gtataatcca tcatggttga gccacgcgaa cagttttttc aagatctgct 63240
ttcagcagtg gatcaacaaa tggacactgt aaaaaatgac ataaaagaca ttatgaaaga 63300
aaaaacgtct tttatggtat cattcgaaaa ctttatagaa cgttacgata ccatggaaaa 63360
aaatattcaa gaccttcaga ataagtacga agaaatggcg gccaacctta tgaccgtcat 63420
gacggataca aaaattcagc ttggagccat tatcgcccaa cttgagattc taatgataaa 63480
tggcactcca cttccggcaa aaaagacaac aattaaggag gctatgccct taccttcatc 63540
aaacacgaat aatgaacaaa cgagtcctcc cgcctcaggc aaaacaagtg aaacacctaa 63600
aaaaaatccc acgaatgcga tgttcttcac gcgtagcgaa tgggcatcct cgaatacttt 63660
tcgagaaaag tttttaacac cagaaattca agccatattg gatgagcagt ttgcaaacaa 63720
gaccgggatc gaaagattgc atgccgaggg tctttacatg tggagaaccc aattctctga 63780
cgaacagaag aaaatggtca aagagatgat gaagaagtaa tatttttggt aaaaatattt 63840
ttatcaaaat ttttttacca aataataaaa aatatttttt actttttttt cttcataata 63900
tacatagaat gcctacaaaa gctggcacaa aaagtaccgc aaataaaaaa acaacgaagg 63960
gctcctccaa atctggttct tccagaggcc acaccggcaa aacccatgct tcttcgtcca 64020
tgcattccgg gatgctctat aaagatatgg taaatattgc tagatctaga ggcattccga 64080
tttaccagaa tggatcgcgt cttactaaaa gtgaattgga gaaaaaaatt aaacggtcaa 64140
aatgaatata atcaggaaac ttaagcctgg aacaattagc cttgtgctgg gacccatgtt 64200
tgccggcaaa actacgtttc ttattcattg catttacatg ctcgaacgtt tggaaaaaaa 64260
agtagtcttc ataaaatcta ccaaaaacac ccgagacaaa actattaaaa cacactccgg 64320
tatacagcta cgacccaaac aatgtaaaat catagaaagc acacagttat ctgacgtggg 64380
ttctctcacc gatatccatg cagttgtcgt agatgaagcg catttttttg acgatttaat 64440
cacatgccgc acttgggcag aggaagaaaa aattattatt cttgcgggac tcaatgcttc 64500
cttcgagcag aaaatgtttc cgcccatcgt tcgtattttt ccttactgca gctgggttaa 64560
gtatattggc cgcacctgta tgaaatgtaa ccaacataat gcatgcttta atgtgcgtaa 64620
gaacgcagac aagacgctta tccttgcggg aggaagtgaa ctgtacgtaa catgttgtaa 64680
caactgtcta aaaaatacat ttattaagca gttgcaacct attaaatatt aaaaatctta 64740
tacaataatg gatcattatc ttaaaaaatt acaagatatt tatacgaagc tcgagggtca 64800
tccctttctt tttagcccgt cgaaaaccaa tgaaaaagag tttattactc tgctaaacca 64860
ggccttggcc tcaacgcagc tttaccgcag catacaacag ctgtttttaa cgatgtataa 64920
gctagatccc attgggttta ttaactatat taaaacgagt aaacaagagt atttatgcct 64980
gttaattaat cctaaactcg ttactaagtt tttaaaaata acgagcttta aaatttacat 65040
taatttcagg ctgaaaactt tttatataag tcctaataag tataataatt tttacaccgc 65100
tccctctgaa gaaaagacta accatcttct aaaagaagaa aaaacttggg caaagattgt 65160
tgaagaagga ggagaagaat cctaagtcgc ttacattttt ttttgctatt tttatagaat 65220
gtacacgcat gttgatgttg tcggaatagc tgaagcctca gcggccctct acgtgcaaaa 65280
agatagggat cgctacttag acgtgctaac aaccattgaa aactttattt accaacacaa 65340
atgcatcata acaggggaaa gcgcccacct actcttttta aaaaaaaata tttatcttta 65400
cgaattttac tccaacaatg tggcggagca cagcaaggct ttggcgaccc tgctttataa 65460
acttgatccg gaatacctca ctcgttacac agtactcatt accaaaattc ccaaccattg 65520
gtatgtgatt aacgtagatc agcgagaatt tgtgcgccta tatgccatcc cggcagttaa 65580
acaacactta ccgattccca ttttaccctt ctattgcacc agcgcactca cccagcaaga 65640
attgttttgt ttaggacctg aactgcagtt aatacaaata tattccaagc tctgtaaccc 65700
caactttgtc gaggaatggc ctacgttgct cgactacgaa aaaagcatgc ggatgttatt 65760
tttagaacag tttccgcaaa gattggaaat gacgggcggg aagaaggagg agaaggaaaa 65820
gcatgaaagt atcattaaaa aaataatact agaaatggtc tctacccgtc agcgaatcgt 65880
tgttgggggt tacatacaaa aaaacctgta caaccatgta ctcaagaata gaaatcgttt 65940
acagcttatt acgagcttaa atatttatga agaaaaagat atcatccagc aattttgtga 66000
ttcaaatgga ctgaagatca aaatacgtat caacaatccg ctcttgccta caaatccgga 66060
attacggcgt ttgactattt attttaatca taataatgat gatgatcagt catatctaat 66120
agtagatatg tacaacacgg gaagctatga gctagtgcct acaaatcaga taaacacgct 66180
tgatggcagc tttttaatag gaacaccctt cgtgcaagcg cgatttttgt tggtagagat 66240
ctgggtgctt atgcttattg cgcagcaaac taaaaaggac accaaaaaaa taatacaatt 66300
ttttataaat caatatgaaa tgcttatgaa tagtccttgg cccagtatgg aggccctttt 66360
tccctcaagc agtaaaagat atttaggcaa ctatgtagac cctaacgcgc tcataaagtg 66420
ggcacaactc aaattaaaaa gaataccgcc tttttatcct ggaaagccgg atgaagaatc 66480
atgttaagcc gattaaaaaa tcatgttaag ctggttgaaa aatcatgtta agctggttga 66540
aaaactcttg gtgaaagcac ggatgtaata ttaacattgg ccgctcgcat ttcgtgttga 66600
aatacgatgg aagagcgacg gctatctacc atgccgatat cggcctggac atcacagttc 66660
atgcacttgt agatgggatg actcgcgtta tagatggcag gctcgccaca gtttctacag 66720
atgtaggaga tgcagccatc cgagtcgtcg tgcgattttt ctatgatggt ttgcatggcg 66780
ccctgcgccg taagcaccca atgctccatt tctcccagac gaagacctcc gtgcgatcgt 66840
ttgccgtcca acggctggcc tgtgagggca tccgtgggcc catagcttgc aacggcgtat 66900
cggtcatcca gcacaaattt ttgcaggcgc tggtgatagg tcggtcctat gaagatggcc 66960
gcatcaaagt actcgccggt ctggccgttg aacatttttt ggcatccatt gaagcgtaga 67020
ccttcttgcg ccagtctttc tgaaagaagc tgcacattaa taggcaggaa tgcggtgccg 67080
tctgttacca ccccctgtag ggcatttgct agaccaaccg tggtttctat catttgaccg 67140
ttggtcattc gggagggatg tgagtggggg tttacaatga ggtcgggctg caatccgtcc 67200
tctgtgaagg gcatgtctga agtgggcagg gccagcgccg caatgccctt gttcccgctg 67260
cgagaactca ttttgtcgcc tatattgaga tttctttcat agcgcaggcg catgaggcca 67320
aagatctcgt cattaggccc atggggacgc atcacagcat ccacgacggc cggctcatcg 67380
aagccgtaca tgacagaccg gtcgatgtat ttgttgagtt cgtctttttc gccccgtatt 67440
ttggccactt ttcctataat gatgtcgccc tttttgacca ccgttcctac gggcacgaat 67500
ccatctacaa gcttttcgta attagcacca ggcttaagat ttttggtgat taaagggtcg 67560
ggcttcccaa acgactctat atcgctttct aattctactt tttcttctcg gtagaaggtg 67620
ccggcaaagc cgcccctgtc aataaaggac tgcgacacga tcacagagtc ctcctgattg 67680
tagccgccgt agatcatata agccacaatg gtattaagcc cgttgggtat gacatagtta 67740
tgtgctatgg tctttacaag cggcatttca ttgtaaaact ggaagaagcg gttcatgtcg 67800
acacgatatg gccagctaaa gcaataccag ccccccgttt gccggccttg gtttgtttca 67860
taggtaacac gcgcaggttg ggtacagttt gcgtaggggg acactagggc ggcaaggccc 67920
aaaatagctt ggggcacgtc cacgtgtgtg aaacgacgcg ttacatcatg tttatgtttg 67980
cgtagctcga tgatggagaa ggcaacaaga cagttttccg cctcctcggg ggtaatgaac 68040
tcacagatgc cctgtgctac gagatcttca agtgtaagcg ttccggctaa aatgtctttt 68100
gccatttgag gcgtaaatcg cgtattttga atgaaaggga ttttatgttt ttcccagtct 68160
ttatcgcctt tttttctggc ctctgcggcc ttgtagcagg cttgattgta tttttcaata 68220
ttattatcta caatgagtag ggggcgggtc agcctaccga cgtccaacca aaattctact 68280
tcgtctacca tgctatccca gtagatggtg gtatggggat gcacaacctt gccctcacgg 68340
cgaagcattc tataccgctg agcaagctca aaggcattgg tgcagcagcc gatccattct 68400
ccgttgataa atacgcgcgc taggcccttt cgtacaatgt ccttgttgga aacatcggct 68460
aactgttgaa tggccggatc tgatagaagg cgttgtttta acgaaagtac ttctccggcg 68520
gtgcagacat tggcagtgat ggctaactgt ttagacatgc ctactttttc accagtatcg 68580
gctgactggg ctacgcagat gtatccagga taggatgcgt gcacgcgacg catcatgtca 68640
gccctttctg tttgtttgga tgcgttggtg gtgttatgag tatttaccgt acgcaatgct 68700
gaaatggtat ttaataaatt ttttctttcc aaactttgag tagatactct gtttacaatg 68760
gggcgctgtc gcaccatgat ggttttattt cctgaaatga tagactgttc catactgcga 68820
ttaagatcgg aggcggtatt ttttgataaa gcggcagaaa atgcctcgat aatgtttcgc 68880
tgagtaagct cctcaaaggc tgtttgttta agaagttctt tgaacccatt gatgatgggt 68940
gctatcacgg aagtattaaa aatagcctta aaggccttgg cgagtgagac ccctgagccg 69000
tgcacccgct tggtgcggta gctatcacgg tccgtgggtg gaaacacatt cataatgaca 69060
agaagtattt tatgaataag caggcctaaa aagcgcagct ttcgtacacg tgtatctgcg 69120
gtttggccca tgtgtggcag caatattttg tctaaaatag taagttgtct ttcatttaag 69180
tattgtaccg cattttcatc gcttttgtaa gcagatgggt ttgagacaaa tttggaaacc 69240
ttctcggata aaaactggat aattttttct cggttcagct cgtgttggac cggttgaaat 69300
atggggtcta aaacatgaat ggatttttcc agaatttcta tcatgaaggt attcacaagg 69360
gagttggatt ctagatcaaa taccacttgc tcaatgatgc tgtcatcgcc tgtcattcca 69420
aacatgcgaa agatgagata ccaaggtatg cgaagttttg agaacttggt gctattgatt 69480
tcaatggtaa tggcgccggt ggtcatgtag cgtataataa tttgagagct attttcgaag 69540
gcacctcccg gttgggagat aaactcgccg cgaatgattt cattattccc ttgttgcatg 69600
gtatggtaat ggatgtgaag cgtgttaaag cggatgtttt ctaagaggtc tacgacccat 69660
tccccgcctc gggctataaa gtagccgccg ggttcattag ggtcttctcc tatttctttt 69720
tttgcggttt ttgataggtg atgagtgtgg cagcggttgc tgccccgcat gatgggaaat 69780
gtagatacct gaaaaggagg aatacttgct cgttttacct cctgccgacc attgctgtag 69840
tgcgccgtta aaataacctc ggcggctaga ttaaccgggc ccgaatagga aaggccacac 69900
aggcgtgcct tattgggtag taaatttatc ttgtttccct gtgaatagtt tcgatgttgc 69960
gggcgttcaa tgttcacatc tgtaaagtta aattggatct gaactgattc ccgaagctta 70020
tctatttcag tatggtcgcg ttggtcttta taagtaatat ccacgttaaa catttgtttt 70080
acaatttgcg gaattccatt gtccataaga tcgtcgaagc ttttgatgtt ataccctatc 70140
aatcctgtag agtttactgc agcggagata aagctcagca tatcagcctc tgtaagctcc 70200
tcattatcca cggtttcaat ggggccgtag gttatttgcg gccgcaaggg ttccatgatt 70260
atgaagtact acattaatat tcagttattc tttaaaataa atctttattt ataaatctta 70320
tttataatat aagaatgcct tatgcaagag acatcacaaa gtttattacg gcaacggaac 70380
cagaggtggg tcttcccctg ttggcgctgc agcgctccaa atccatcata ggggttattc 70440
ttcttgtaat aagtttgtta tttattttca ttggcattat tatattatca gtgagtagtg 70500
gtcataccac agcagcctct atatttatcg tattgagtct tatcctaggt ggcggtggtt 70560
tttttcttat ttataaagat aattcttaac ccacataaaa tttgaaaaaa tatagagtaa 70620
gaaaatgtcc aattactatt attactatgg cggggggaga tatgattggt taaaaacagt 70680
agaacccact aattttttaa aaatcgggtt gccttaccag gcacacccat tacatcttca 70740
acatcaggca actactcccc catctatctt agaaaaattt aaacgagcag acattcttct 70800
taatgaggtg aaggccgaaa tggacccact catgttacaa ccagaaaccg aaaaaaaact 70860
attccagata ttgagtagta ttgatatgtt caaaggtctg cgaaaaaaag tagaattcac 70920
gtacaatgct caaattgtta cgaatgcttg gcttaaaatg tatgagctgc taaataccat 70980
gaattttaat aatacatctc aggcattttg caattgtgag cttccaggag ggtttataag 71040
tgcaattaac cattttaatt atacaatgat gcattaccct acttttaact gggtagcttc 71100
ctccctttac cccagttcgg aaacagatgc cctggaagat cactatggtc tttatcagtg 71160
caatccggat aactggttga tgcaatctcc tttactgaaa aaaaatatag attataataa 71220
cggggacgta accatcgcta gcaatgtaaa aaacctagcg cttagagcca cacaaaggct 71280
gacgcccatc catctatata cggctgatgg gggtattaat gtaggacatg actacaataa 71340
acaggaagaa ttaaatctta agcttcactt tggtcaagcc cttacgggtt tgttgagtct 71400
tagcaaaggc ggaaacatga tactcaaaca ctatacctta aatcatgcat ttactctttc 71460
tttaatatgt gtattttctc acttttttga ggaactatac attaccaaac ctacctcctc 71520
tcggcccaca aactctgaaa cctatattgt gggtaaaaac agattacgct tatttacccc 71580
caaggaagaa caagtccttc taaaacggct agaatttttt aatgatacgc ccctcgtaga 71640
cctaagtctt taccaaaatt tacttgaaag cgtttacttt gccgtagaaa caatacatct 71700
aaaacaacaa atagaatttc taaacttcgg aatgaaatgt tatcgacatt tttataacaa 71760
gattaaacta cttaacgatt atttagctcc gaaaaaaaag atttttcagg ataggtggcg 71820
tgtgcttaat aagctttatg ttcttgaaaa aaagcataaa cttaagcttt gtgcctccta 71880
gggatctgtt gcttaattta acagatgcaa tcttaacaga tgtaaactaa aaagtgtgtt 71940
catacaagga ttgtatttat gaatatttat taacatataa ggttgtgatg taacactgta 72000
taacctatat aactacacta tgaagcacgg cgtataataa tttatattga acacgatgtt 72060
gactcattta tttgcaaaca aatatttgtt tgcaagacgt ttgcatgcat ttactaatat 72120
gttgttgact agtttatttg caaactagat gtttgattgc aaactagatg tttgcacgta 72180
tttatttgaa ctaatataca ctccttgttt tatttgttat atacacagca tacataagtg 72240
tatattgttt acacttatgt ttataactcg acgtaataac attttacacg cttttttttt 72300
gcaaatctta ataatattgt atgataaatc aaacaatgtc ttatatatgt ggtttattat 72360
tttaggcgcc gcaagatgta ctccattctc attgcatgct tggtgttatt actctgtcta 72420
gttatatatg tcggtcatcg tgccgatcat gcacgaaaat atttagaagg aatgtggcat 72480
ggagatccgg tttttctaaa acagtcgggg ctacaatcct tttatctcta catacaacct 72540
gaccatacat gtttttttag cattgtgaat aaaaatggtg aaaagctgat ggaaaccaaa 72600
ataccttgta cgataacaaa taaaatatat atgtttttta aacctatttt tgaatttcat 72660
gttgtgatgg aagacataca tagctacttc cctaagcagt ttaactttct gttagatagt 72720
acagaaggta aacttatttt agaaaacaat cacgttattt atgctgtatt gtataaggat 72780
aatttcgcca ccgcactagg aaaaacggtt gaaaaatata taacacaaaa ttaatcatgt 72840
tttctaacaa aaagtacatc ggtcttatca ataagaagga gggtttgaaa aaaaaaatag 72900
atgattatag tatattaata attggaatat taattggaac taacatctta agccttatta 72960
taaatataat aggagagatt aataaaccaa tatgttacca aaatgatgat aagatatttt 73020
attgccctaa agattgggtt ggatataata atgtttgtta ttattttggc aatgaagaaa 73080
aaaattataa taatgcaagt aattattgta agcaattaaa tagtacgctt actaataata 73140
atactatttt agtaaatctt actaaaacat taaatcttac taaaacatat aatcacgaat 73200
ctaattattg ggttaattat tctttaatta aaaatgagtc agtactatta cgtgatagtg 73260
gatattacaa aaaacaaaaa catgtaagtt tattatatat ttgtagtaaa taatattttt 73320
aattacttaa aatttttata tataagtttt tgatactata ttataaaaca tatgttcata 73380
aaatgataat acttattttt ttaatatttt ctaacatagt tttaagtatt gattattggg 73440
ttagttttaa taaaacaata attttagata gtaatattac taatgataat aatgatataa 73500
atggagtatc atggaatttt tttaataatt cttttaatac actagctaca tgtggaaaag 73560
caggtaactt ttgtgaatgt tctaattata gtacatcaat atataatata acaaataatt 73620
gtagcttaac tatttttcct cataatgatg tatttgatac aacatatcaa gtagtatgga 73680
atcaaataat taattataca ataaaattat taacacctgc tactccccca aatatcacat 73740
ataattgtac taatttttta ataacatgta aaaaaaataa tggaacaaac actaatatat 73800
atttaaatat aaatgatact tttgttaaat atactaatga aagtatactt gaatataact 73860
ggaataatag taacattaac aattttacag ctacatgtat aattaataat acaattagta 73920
catctaatga aacaacactt ataaattgta cttatttaac attgtcatct aactattttt 73980
atactttttt taaattatat tatattccat taagcatcat aattgggata acaataagta 74040
ttcttcttat atccatcata acttttttat ctttacgaaa aagaaaaaaa catgttgaag 74100
aaatagaaag tccaccacct gaatctaatg aagaagaaca atgtcagcat gatgacacca 74160
cttccataca tgaaccatct cccagagaac cattacttcc taagccttac agtcgttatc 74220
agtataatac acctatttac tacatgcgtc cctcaacaca accactcaac ccatttccct 74280
tacctaaacc gtgtcctcca cccaaaccat gtccgccacc caaaccatgt cctccaccta 74340
aaccatgtcc ttcagctgaa tcctattctc cacccaaacc actacctagt atcccgctac 74400
tacccaatat cccgccatta tctacccaaa atatttcgct tattcacgta gatagaatta 74460
tttaatatgt actatatatt aattatttaa cctttcaagc tggtcttcat ttaaatttaa 74520
aatccactaa taaaatgtat tttctagtag cagatcatcg agaacatcat gtgattcctt 74580
ttcttaaaac cgatttccat cacatgcatc aaaatcctat acaaaaaaat caagctctcc 74640
tagaaatcaa acagcttttt actggagatt atctcatctg caaaagccct tctaccattc 74700
tggcctgtat tgaacgaaaa acctacaaag actttgcggc ttctttgaaa gatggacgtt 74760
ataaaaatcg ccaaaaaatg ctgtcgctgc gagaacaaac caactgtcaa ctttattttt 74820
ttgtagaagg cccggcattt cctaaccctc aaaaaaaaat taatcacgtt gcctatgcaa 74880
gcattattac tgctatgacg catcttatgg ttagagatca tatttttgtc attcaaacga 74940
aaaatgaggc ccacagttcc caaaagcttg tgcagctttt ttatgccttt tctaaggaaa 75000
tggtgtgcgt cgttcccacc tccctcaccc ccacggatga agagctatgc atcaagctat 75060
ggtcttctct ttctggtatt tcaggcgtga taggtaaaat cttggcaaac acttgttccg 75120
tagctcattt ggttcatgga aagctttcat cgcagaatat tgatcagtta aaaactccct 75180
ccaaccgacc attccccaaa aaagtaaaac gtatgcttat aagcattagc aaaggaaata 75240
aggagttaga aataaaattg ctctcggggg ttcccaatat cgggaaaaaa ttagctgccg 75300
aaattttaaa agatcatgcg cttctttttt ttctaaatca gcccgtagaa tgcttggcaa 75360
atatacaaat cgttcaaaaa acccgtacga ttaagttggg aatgaagcga gccgaagcga 75420
ttcattattt tttaaactgg tgtggctctg cccatgtaac cgatgatagc caaaatatca 75480
cagaggcgtc gcggtccaca atgcaggtcg cgacgcagtc cgccgcaata cagcccgctg 75540
caacgcagcc attgcacgaa gtatcagatg atgcatcatc agatgcttca tcacccgtag 75600
ggtatcaaac attatctaaa gaaatgttat tgaacacagc ctgatgttaa taattcacta 75660
catctaaaga aatgttaacc tcgatactaa aaagtcattg aacacaacta ctggggcgct 75720
aagttgtcca acacatctaa agaaatgtca acatcctcga tgctaaaagg gtcatcgagc 75780
cggtcaataa tgtcttcccc aaaaagtccg ggagaactgt aggccgagat gtcgtccatg 75840
gagctatctt ccccagagca cacaaagtcc tctccaaaaa tcataaagtt aaatgcaccg 75900
ggcttactta acagcttttc gctttgaata atagtgttga gttctgtcag cgcaaactct 75960
ctcacaatat tcacaaccca ggagggctct ttaatttcat acagcgttaa gaaacttata 76020
cataaaaatt ctatagagta aagcaaggcg ctggcaggat ctgttacccg taggtgttta 76080
aatgtagtgt gatattcatt cacaacgtta ggcagcacct tttccaaatc ctccttttcc 76140
tcgtacgaca ggtgctttac aagcctttca acatgtatag gaggcttgtt aaatgtacta 76200
acgtgccgca aacagttata attatataag aaaatacgta cggcagagtc gaccgccatg 76260
agccttggat catccattga ggtaggtggt ggcggggcac cctggccttc cctgatgtct 76320
gcgtaggagc gcccctccat ggcccctatg gcctctatca cagcaggact gatatccaaa 76380
atcttggccg tcttgattat ttttccgtaa tcgaaagtcc atggctcctg tggaggcttg 76440
ggttgtgttt cggtggaggg cgtggtcata tctttcttta tttgaataga acggatcgac 76500
atcttttcct tatcgtactg gtctttataa ttattataat agtcatgaac taattcgggt 76560
tgagaaagat gatcgtatat aatataggta aaaagtccgc acttgacaca ttttttatcc 76620
tggaagtcgt gtaatcctcc cttggggcag cgtgactcgt agaaggcata aaaggtgtta 76680
aattctaagc tcgcctttag ggctgtttgg acctttttta tgtttaattg ccccacctca 76740
tgttgtagca cgtggcatac agaacagcgt agatcggcaa gtgcataatg gttgtcaatt 76800
ttttttatga cgtctttgcg tgttacttca atctcggcgg gtttctgcga actgtctacg 76860
gccttgtaaa cgtaaatggt ccacttatga ggaagccccc tttcatcgta tagggttgaa 76920
atgggaagcc ttttatactc aaacagccga gtccgttggt cggctcttcc tgtgttagga 76980
tcaaatatgt tataaaatcc ttgctgagca agcagggcct tttgctcgcc ataagcattt 77040
tcgtacgttt tgaattctgc aagttcggag ttaaaattag gtgcattttg taaatactta 77100
agaaataatt cataggctct aaggtaaatg agagttgagg ttttttcctc atcccgtcct 77160
ccccaccaca cccgcaggct ttcttcttga aaatagatgt cattcagacg cgtcaactgc 77220
gtaaaatcag gccgatattt agaggtataa attttatcat aaaattcttt ttgcgataat 77280
agctcggccg gggtacgtcc tatcacggtt ttaaactcat attcagcctc cttgggagtc 77340
cgtggtttgt gcatagggat gctgccgtca atacgggcca ctgtggcagc ataatcatac 77400
atggggtcca gcagaatctc tgtcaaaagt accttggtgt cgtcctgcac gctaagccct 77460
tgtagcccat tttggtggat aatttttttg aaagcctccc gaaaattatt agcaatccac 77520
tgatccgtaa tctcagatag ctgatttatt ataccgctat attgctgcat cattttctcc 77580
aaaagaaagg tcacgtatgc attcaaagag ctatccgcct tcattccatg aatggtaatc 77640
gtaagaaatt ctttattttt ttgcgagcta taaatgagat tcaaaatata ggcatagatg 77700
tagatcacag catacagctg cgttaaagga tcgtaatcct cttccttttt aatattttcg 77760
atgctataca cgagcggcag gcagacattt acggctatat tggcaaactg tttcacgtct 77820
acaagctttc caaagtggat aaacgtgcag gccttcatgg tttcctgcca aataaaaaca 77880
cggagcttac tattaagatc gccgatgatg cccacatctg ccgtacgatc ctcttgaata 77940
aaatgggcca gctcttcgcc acaaattttg caaaagtagg agtaaataag cccctggttg 78000
ttttctttct ccttgtttat tcctgaaaat ttcattagct tggttcgcat ggtgtcgtag 78060
gacgcttctg ccgcttgaag ctgtataagc atgtccacat ggggacaaag cagcttaaac 78120
ccgcaggctt tgcatagatt ccaattggtg gtattgtttt tttccttgta gagtacacga 78180
atactttcta atacttttaa taactccgcg tattgaagac ccgaacgcaa ctgttttacc 78240
agcttgagat gagcacatgc atttttttct tggagttccc actgtttttt aatgtttagg 78300
tattctgttg taataagttc tgcctcctgt ttcccacagg ctttaatgac ttcttgaagg 78360
atgctgttag ggtcatccac tttaccctcc attgtaagaa tttcacgtat agcatccgac 78420
tgcaccctac ctattttttc ttccataatt ttaaaatact gtctcgcctg ggtaatgacc 78480
tctgtgagct tcatgtccac ctgctgcaga atcatttgct ccttttcacg ctgttcagca 78540
tgttgtaaaa acttttgttc tacagggttc caaagcacct ccaaatagcc tgctctatat 78600
aggtcataaa gcaagggcat gtatcccgat gtaaaaaccg gggacaccga gtacatcgta 78660
gacaactctt ttaaaaaaaa tatcacgcgc ttaatgttct cctccggttc aatctcctcg 78720
gtttcaacga tattagatat atgactgccc tgatcctcac ggtctagctt tcggtgtacc 78780
atctcctctg ctagccgatt aatgagccag ctatgcccgc cgctccgcaa aaacttataa 78840
agttcgatat actggtgcgt aaactggatg atgttttcct tggtggttac gacaacccct 78900
tctccgtttt ttttccaggt ttcttgatcc acgcatttca taaatactcg aataaaattg 78960
gtcaaattgg ctcctgaggc gacgtagccc aaggtttcag gcgagaagga gcctatctca 79020
gccatacgca taaaacactg cggggaaaaa gtttttagcc gcaacttaag tccatagatt 79080
tcaatggggg cttctgcggg aacggccagg tgcgtcccat taattaaaaa aatttctttg 79140
cgtgtgctag ggcgaacacg taattccttt tttttttcac tcacgatggg gaccacatcg 79200
gggtctacca gcagttgacg tatgtaggcc tctatgggca tggatagatc gggcagcttt 79260
gactgctcgg cgcgaacatg gttcacaaaa tcttttagag tgaaaagaaa gtctattaaa 79320
cgtatgtttt ttatatcatt agacccttta agggtagagt agatttcatc cactagtgcc 79380
tcgatttcct cattattgag cgataagata tctgtgccac ggtggactat ttgcgcgatc 79440
gtaattactt cctccattag atagaaactg aatattatat ttaaaataaa tacaaaatgt 79500
caaatgaaag ttttcccgaa acgttggaaa acttactttc aatgttacag accaaacagc 79560
aaaacgcaat tcagtcagag gtgattgaat ggctgcacag cttttgtgaa acctttcact 79620
taaaaataca ctgccataaa cagtttattc ctagcgggga aaaaaaacga gctaaaatac 79680
ccgctcaaga aacacaggga aacacgcagc cctcccacca tgtgtaccgg gttgttctct 79740
ccagagcaca gccagtcaaa gcacaggaat ctctgctaac aaccatgtgc aacggactgg 79800
tgctagatgc aaacacatgg acatgcctag ccattcctcc gcctgcgccc tttcaacagg 79860
cgacccgcca ggtccaacac ttttaccgta acaatttcta cgaagtggtt cccatccagg 79920
atggcaccct tctcacaatc taccactggg atgaccctga atatggcccc tcctggtgcc 79980
tagcaagtac ccacggatat gatgtgagta actactgttg gataggcgac aaaaccttcg 80040
ccgagcttgt atacgaattg ctgcagcagc actctacctg cgacgtcacc ctggaaaaaa 80100
ataaaacgcg gggaacgcgt cttttctttg ataacttaaa tcccgattac tgctatacga 80160
ttggaatccg gcaccataat ttacagccgc tcatctatga ccctcaaaat atttgggcga 80220
ttcaatctac aaacctaaaa acgcttaaaa cggtatatcc agaatactac ggctatatag 80280
gcattccagg aattcagagt caagttcctg agcttcccca gtatgattta ccttatctaa 80340
tacgatctta taaaactgct atgaatcaag ccaaaaatgc tataaaaaat ggcaaaaaag 80400
acaagggata ctttaattat ggctatttac tcatttcgcg agcgcctgcc attactaaaa 80460
gtacttctaa tgttttgtta aaatcgcctc tgctggtatt tttacaaaaa agtgtgtacc 80520
agaaaaaaca caatatctct aacagccagc gactagaatt tattatactg caaaactact 80580
tgatgcagca ttttcgagat catttcattg ctctatttcc gcagtacata tcctattata 80640
cgaaatacca aaacatgttg aatatgatta tccatagtat tgcaactaaa gataaagatc 80700
atccctttgc aggagccgtg gtaaaaaaag tgttggaaga tattgaaaac gccgaaaaca 80760
ttattgatca tacaaccatt caaaactatg cccatcaaag caagtacgcc atgctttact 80820
tgtcaattat ttcccatttt taatctaata cggccaaagc cgcgggtttt ttaataaact 80880
aacatttaaa aaaactgttt tattaaaaat tataatactt ttattatata tggaacatcc 80940
atctacaaac tatactcccg aacagcaaca cgaaaaatta aaacattatg ttttaatccc 81000
taaacacctt tggtcttata ttaaatacgg aacgcatgtc cggtactaca ccacacaaaa 81060
tgttttccga gtcggtggct ttgtgcttca aaatccctac gaagccgtta taaaaaatga 81120
ggtaaaaaca gcaataagac tgcaaaatag ttttaacaca aaagcgaaag ggcatgtaac 81180
gtgggccgtc ccatatgata atattagcaa gctatatgcc aaaccagatg caattatgct 81240
taccatacaa gaaaatgttg aaaaagctct tcatgcttta aaccaaaacg tactgacgct 81300
cgcatcaaaa atacgttaaa tataattttt gtagaggata aaaagctatt ttagctaaaa 81360
aataattcat atacgtttat gcagaggaag aacggtggct ttcaaattca gattgcatcc 81420
acgtagaccg tagcgttttt tttgcttctg gtttatatcg taaaccgtaa taaacatcat 81480
catttgtatc cgttggatct ttttcccact ccggataaaa aatcggtttt cttttttttt 81540
ggtcgttttt tgcagtaagc tgtaaattaa gggaatatag cttatcgaaa agttgttcct 81600
gatccatata aatagcagca tatattaaaa aaaaataaaa aaagacgctt caacgagtca 81660
gtaccactgc ttgccaacga tttacgttgg ttggtgcatt atggtgatat agtaatgagt 81720
gcctgcacaa gtgcttgcac aagtgcctgc acaagtgctt gcacaagtgc ttgcacaagt 81780
gcttacacaa gtgcttgcac aagtgcctgt acacattact gcatcgccaa agcacctgca 81840
atgcctactt cctcaacaga gtacgataac taaatgcttt taagcaccgc ttgcgtcgat 81900
gtgtccttcg gggcaatcgg gttcaattgg atccaatatt attagtcata attacctaat 81960
acttattcaa ttttatcttt tttaccttgt aagatttaaa cagcgtttta gcttgtttaa 82020
agcaacgttt aaaacaagct aaaatgctgt ttaaaacaac gttttaaaca agttaaaaca 82080
aataagctta taaatatacc atgacaaaat tagcccaatg gatgtttgag cagtatgtca 82140
aagatttaaa cctaaaaaat cgagggtccc cctcgttccg caaatggctc acattgcaac 82200
cctcactgct gcgctattcg ggtgtgatgc gtgctaacgc ctttgacatc ctaaaatatg 82260
gctatcctat gcagcagtca ggttatacgg ttgctacgct tgaaatccac tttaaaaata 82320
ttaggtcttc ctttgccaac atttactgga accgtgatag cgaggagcct gagtacgtct 82380
gctgttgtgc cacctatcaa tcgcacgatg gcgaataccg gtatcgattt gtttggtacc 82440
aacccttcat agaggcttat aatgccatag aggcggccct ggatcccctg gaaaccatta 82500
tcctgaacct cattgcggca cgagatctag acttcgttgt tcacatattt ccttataata 82560
agggccatga agactatttg gcctccacgc aacttattct caaaatcttt attgcgacgc 82620
ttttaatgga cattttaaga attaaagaca acacgttgga cgttcactta aattccgact 82680
atattattgt gatggagcgg ctttggcctc acataaagga tgccatagaa cacttttttg 82740
aagcccataa ggacttacta gggtacttaa ttgcctttcg caatgggggg aactttgcag 82800
gaagtcttag accctcctgt gggcaaaaga ttgttcccct aacgattcga gaggtcctac 82860
aaatgaatga tattaattta gccgtatggc gggaggtgtt tattatgcag gaatgttccg 82920
acttagtcat caatgggata gcgccctgtt tccccatttt taacacgtgg acgtatttgc 82980
aaggtattaa ccagattttt tttgaaaaca cgtctttgca ggagaaattt aaaaaagatt 83040
ttattgcccg agagctttcc aaagaaatta tcaagggcca aaaaacgttg aatgacaagg 83100
agtttaaaaa gttaagccta catcaaatcc agtacatgga atcctttcta cttatgtcgg 83160
atgttgccat tatgattacc acagagtatg ttggctatac ccttcaatcc ctgccgggta 83220
ttatttcgcg atccagctat ttatccccca tcgtgaaaaa cattttgatg gacaaagact 83280
cttttatgtc cctactattt gacctatgct atggcgccta cgtgttgcat aaaaaagaaa 83340
atgtgattca cgcggatttg cacctgaata acatgaccta ctaccatttc aacccaacca 83400
gttttacaga tcgcaacaaa ccaggaaaat acaccttaaa ggtcaagaat cctgtgattg 83460
cctttataac cgggcccaaa gtcgaaaccg aaacgtacgt gttcaagcac atagatgggt 83520
tcggctgcat cattgacttt agcagagcca ttatggggcc aaaccatgca atcaagcttg 83580
agcggcagta cggcctcgct tttgtaaaca ccttttaccg caatcaaagt gagcatattt 83640
taaaggtatt acggtactat tttcctgaaa tgctaaccaa tcgcgaaaac gaaatacagg 83700
gggtgatttt atcaaacttt aatttctttt tcaatagcat tactgccatt gatttttacg 83760
ccattgctag aaacctacgt agtatgcttt ctttggacta tttacacacc tctgaggtga 83820
aacgaaacgt agaaatttcg caaacatttt tggatacatg tcaatttttg gaggaaaagg 83880
ccgtggaatt tttgtttaaa aatcttcata ctgtcttatc tggcaagccg gtcgaaaaaa 83940
cggccgggga tgtgctttta cccatcgtat ttaaaaaatt tttataccca aatattccta 84000
aaaatatatt acggtctttt accgtaatag atgtatacaa ttataataat ataaagcgtt 84060
attctgggaa agctatacaa acgtttccac cctgggctca aaccaaagaa atcttgacgc 84120
acgccgaggg tcgtacattt gaagatattt ttcctagagg agaattagtt tttaaaaagg 84180
cttacgcaga aaacaaccat ttggacaaaa ttttacagcg tattcgtgag cagcttgcta 84240
atgaaaattt gtaaggcttg cagttcttgt atggtcagaa cctatgtcga tggaaacatt 84300
atttttcgct gcagctgcgg cgaaagcgtt caaggggata gtcagaactt gctcgtctct 84360
agcaaggtgt accacaccgg ggaaatggaa gataagtaca agatttttat taaaaatgca 84420
ccctttgacc ccacgaattg ccaaataaaa aaggattgcc caaattgtca tttagactat 84480
ttgacacaaa tctgtattgg aagccaaaaa atcattatat tggtgtgccg ctgtggctat 84540
atgagcaaca gaggataaac catatcatcc caccgaatta tgacattcct ttaaaaccgt 84600
ccgcctaaat agttttcaca cctttggtgg cagactattt tataaaaagt aatgttggtt 84660
catgaagata aagtgtgcca aagaaacttt tataaacaaa tgattaatgt aggtgctagt 84720
cgtgtgtact taaacagggt attctatagc caagtatttt ctatagccaa gtattttcta 84780
tagccagtat tagtcaagta tttagatgtc agggtatttt tatagccagt atttttctat 84840
atgtacaaac tattccagta aacatatgtg tgttctttat tgagcagcat catggcatta 84900
acaagtttat taaactgctc taatgggcat taaatgacaa ctcggtgctt agcaaaagtg 84960
cctatacctt ttaacaatta gggccgggag gcattcccag cttttttcta taatcagcca 85020
tacagtaccc ctgagcctca tacacgggaa taaggtcctt ccattccttg ttgggatcgg 85080
cgggccagct ctcaaatgag gtgtgaatgt aagggtcctg ttctttttcc ttaatgaagc 85140
gtttaatctc catttgatgt tgtttacttt tttgtttgcg gcggagcgtg ttccgcacca 85200
atacgtaaaa aataccaaga atcacacata aaagaattat taaaaaaaat atcatcatcg 85260
cggggtttaa aaaacgatcc catgcaacag gaatcgttct taaaaccttg tctggcaggg 85320
ctgtaaacat gaagtctcct cctataatcg gggtgggact gtagcctaac agttcaaggt 85380
cctgtcgttc tagatactta ttggcgaact gcccaccctt tgcccccgtt tttttattaa 85440
tcaagcagcg ctgcattttc caccattcta aatcttcagg agaaagctca atgccatata 85500
tcaactttaa cgttattgca tctttttcaa tatccttatc aatttggctg agcttttgag 85560
ctttaagcgg gtctagtgtg tacttccatt taaacttagt gtcctgtagt ttggctacat 85620
gaaatacgga acatttcggc ggggcctttg tgacgccctt acactgcgga agtttatcat 85680
taggacaggc gcatagatga gactgcgcca cagcatcgcg aactacatcg cagacggagt 85740
acattttcct cctatgttaa acaataaatt tttttcatag ctgaaatttg tgggcctatc 85800
ttttcccttg cccggataat aattataagg gagtgttgaa acatctggga gagaattgct 85860
taaaaaatgg gtttttggga ggggtaactg cgactgttgt acgtcgttgg ccagggagat 85920
tctatatgcc gggctaaagg tgcaacgttc ctgtgaacaa cttagtacgc gcgttgttaa 85980
tacaaatgga ctggtattag caaacctcgt aaactcttcc ggacttgttt gtttttgtat 86040
gatgtttagc agggagtctg ccttttcgag aatccaaagc gtcgcattgt agtaaaataa 86100
aaatagcgac ttatcggcag gcgttgcaaa agcgccgtat agaaaataaa gcagtaagta 86160
ctggggagac accacaataa ggttatcttg aatgatagat atcgctagct ctttaaacat 86220
agtgctaaaa aaatgtatgt cgttcgtctt gaatataggg ggactatagt ccatgtaggg 86280
ctcacatatc tcagtcaggt gaaggcccat ttcttttatg acttcttccg ggttgtacgt 86340
cgctaacacc agcgcgggat aggctttggg catatccacg gtaagtgtta tgtttttatc 86400
attcttatgg taggagtaag atggttgtgg aaattctgtt ttccactccg ggactttgca 86460
ggtaattctc agctcattta gagtctggta caggagggcg tatgccgcaa agccgtgtat 86520
ggccacttgt ttaaagggaa ttgaaaacgt tttactttcg tatgtcgact tcacaggaac 86580
aacgggaatg gggtaatatt tttctatgag gttataccgc tgcaaatcct ttttaaacct 86640
gctaaaaaca tcttcccttg gtgggttatc aaaaggaaag caaaatgcta ggtgtagccc 86700
ggcccgctgg taatcggggt gaatgatttt aaggttttta tacgttaatg tgggtatggt 86760
gttaaagata ttggggggca tatatgaaag atcagcaacc cacacaaagt ccgtgcgcac 86820
ccgcatggtc tgcacatgga tggcgcgcac cgtgcccacc tgcttgaagc ccttttcata 86880
caaaatgtca gcaagttcgt aggcgtcctc aacgtggttg ggggaaaaca tatcaaagtc 86940
gggtctttct ccctcgggat aaattgagct gcctttaaga tgcagggcat aatcaatggc 87000
aatccccccg tacaaaataa gctttttctt tatgataaat tcgcggacca cctccaaagc 87060
cgcctcaatc tccacggcat ttgcctcacg tttttgagca atgagccggt acttagaaac 87120
attaaaatca gtctttagta aagacgtcat aaatagtgtt taatatatat taaaggtttg 87180
aataaaatac taaatagtaa aaatggatgc cctattaaag gaaatagaaa agttatcgca 87240
gccatccttg cagaaagaaa acaatgatgt atgcgatctc tgttttatgc aaatgaaaaa 87300
aatttctaac tatcagcttt tatgcgaaga gtgcggtcag ctgaaggact ggtttgaacc 87360
tgaatataat gaaaaattca cggtatattc tcgtctaaag atcgtgggtg ccaatagttc 87420
ctatcaccag cgcgatttgg acaaggccaa ctcaagtgac tatagctcct tgcaatttca 87480
tcacatttta gaggagctca aatccctaaa tgttaagtat atggatgcgg ggcaaaagcc 87540
ctttcctatt caggtgttaa aagaaactgc tcacagttat aaccaagtac aacaacatcg 87600
ggtcatacgc agcattacaa agcttcagat cttagccagt attctacgta gcatttgttt 87660
aaaattaaac attgcttgta cggtggcaga cgccgcgagg tttactcaac ttaataccaa 87720
agggatctca aggggcatgg atcttctgcg ctccctattt gtagacaata aaattacttt 87780
aaacgttgat ttaaacccta tagacagctt tattaatagt acctacagtg ccttacaaat 87840
taaacaaatc caccaagaac tgcaggagga aaatgtttat aatttaaaag aaattgttaa 87900
gagctttata ttatacgcgg atgagaagaa catcggcgtc gatcttaaca ggagaaccgt 87960
tgtgattgct acgatgtata atgttttacg ccgtgcctac taccccatag aaattgatac 88020
ggtggtgtat caatgtaaaa tacgaaaaaa tacaattaca cgtgctctta aaatgtatga 88080
ggattactac tcccacttta agtctcttta tgagcagtat catttaaacg cggcaaaaaa 88140
attaatttaa actaaacgtt taaactaaat gtttaaacta aacgttaaaa ctaaacattt 88200
cgactaaagt ttaaaaccta gtctaacagc gggatgccca tttccctggg gttccatatt 88260
tcaacaattt tttgaccttc gggtgttacc ttgatgcagc gcatgacgag cagtggaatt 88320
ttcctattaa agagttcttg cttagctata tcaataggac tgctatattt ttttttaagc 88380
attgtagatc cattaattgc caattgttgc gctctaacgg cgaccaacct tgtggcctca 88440
aaggtggtta aaacgttgga ggtaatgcgc tcgttatcgg gtataatgac caatgtttgc 88500
gacgaggcct gcacaaagcc ctcgcagatg gacggagact ccacgatctc gtccttgtcc 88560
tcggactcct cctcactgtc gacgaggttc tcctcttccg tttccacata ttcctccacg 88620
aggtcatcca tgataagatc ctcgttgtca ttatcagcca tattacactg ttatcaaatg 88680
tactgtttaa tacgcaaatg gatttactac gttttaattg tatgtcttca tgtgcaggct 88740
ctagtggaaa gtaattttct cacaattttt ggcaccgtta cacttgtgcc cacaaaaacc 88800
cgcgattttt ttattttata ttacttttgg aagtacgagt ttaaccagtc gctttcaaac 88860
cttatgcgtc tatctcgcca aaaaacgctc acagcggtgt tggatattac ctttaaaaaa 88920
ataacattaa tttttaccac agagggcgta ttgcgtatgg attctacgaa taagccaggc 88980
gtgccactcg atatagaccc ccagttcatt gaccttgata gtattttaat ggaactggat 89040
cattaggacc tctcccgccc atttaaattt ttagtttcta caataataaa atgcgcgagg 89100
aatcatggga agaccacgat accattcagc tcaccgctca gcgcaaatac ctcgccgagg 89160
tgcaagctct agagaccctt ttgactcgag agctttcagt ctttctcaca gagccaggca 89220
gcaaaaaaac aaatattatt aatagaatca caggaaaaac ctacgcactt cccagcacag 89280
agctactaag actctacgag catctcgagc aatgtcgcaa gcaaggcgcc ctcatgtatt 89340
ttttggaaag acaggggacc tactcgggtc tcatgttgga ctatgacctt aaactcaata 89400
caaatgctgt tcccccgctg gaaccccccg cgctatcacg gctttgccat cgaatatttg 89460
tgcatataaa aaacagcagt gtgctgcctg agggcagcca taaaatccac ttctttttta 89520
cattaaaacc tgaagtggtt cagggcaaat atgggttcca tgtgctcatt cctggtctca 89580
agctggcggc ttctaccaaa aaaagcatta taggatccct acagcacgat gccaccgtac 89640
aaaaaattct acacgagcag ggcgttacaa atcctgagtc ctgtctggac ccccactccg 89700
cctccgttcc ctcgctcctc tacggctcct ccaaactaaa ccacaagccc taccaactga 89760
aaaccggctt tgagttagtc tttgatagct ctgatcccga ctacattccc attcatcaaa 89820
taaaaaattt agaatcttat aatttagttt ctgagttgag ccttacgaat gaacagggaa 89880
gccttgtaag acctgtctat tgcgcggcag acattgccgc tgagaaggag gaagagatcc 89940
cgaccgagga tcactcgctc tccatattaa tgctacatga tcccgaagcc cggtatttac 90000
ataaaatttt aaatctgctt cctccggagt attatgtaga gtacccccta tggagcaacg 90060
tcgtattcgc tttggccaat acatccgcta actatcggcc cctcgccgaa tggttttcgc 90120
aaaaatgccc tgaaaaatgg aatacgggag gaaaagagaa actagaaaaa ctttggaatg 90180
atgcctcgca ccacactgaa aagaaaatca ccaagcggtc cattatgtac tgggcccaca 90240
aacatgcccc ccagcaatac aaagaaattg tagaacaagg ctacttttcc attctcgctg 90300
aatatgtgta tagctataac ggcatgcttg agcactacat gatcgccaaa gtcatctatg 90360
ctatgatggg caacaagttt gtagtggacg tggattcaaa cgggaagtac gtttggttcg 90420
aatttgtgct accgggccag ccaatgaatc agggagaaat atggaagtgg cgcaaggagg 90480
taaacccgga tgagctgcac atctatattt ccgaaaactt ttcaagggtg atggaccgaa 90540
tcacggagca catcaaatac cacctcagtc aaccccatga aagcaatatt ttaaattatt 90600
ataaaaaact attaaaagcc tttgaacgct ctaaaagtaa aatctttaat gacagcttta 90660
aaaagggagt tatcaggcaa gctgagtttt tatttcgcca aagaagcttt attcaaactc 90720
tggataccaa tccccaccta ctgggggttg gcaacggggt tctctccatt gagaccatcc 90780
cggctaagct cattaatcat tttcacgagc atcccattca tcagtacaca cacatatgtt 90840
atgtgccctt taatcccgaa aacccctgga caaaactatt attgaatgca ctccaagaca 90900
tcatcccaga acttgatgct aggctgtgga tcatgttcta cctaagcacg gccatatttc 90960
gcggcctgaa ggaggctctg atgcttttgt ggcttggagg cggctgcaat ggaaaaactt 91020
ttctaatgcg acttgtggcc atggtattgg gcgatcacta tgcctccaag ctcaacatca 91080
gccttcttac aagctgcaga gaaaccgcgg aaaaacccaa cagtgccttt atgcggctta 91140
aggggcgggg atatgggtac tttgaggaaa ccaacaaaag cgaggttcta aatacgtcgc 91200
ggctgaagga aatggtaaat ccgggcgatg tcaccgctcg agagcttaat caaaaacagg 91260
aaagctttca gatgacggcc accatggtcg ccgcgtccaa ctataacttc atcattgaca 91320
cgacggacca cggcacatgg agaagactgc ggcattatcg gtcaaaggtg aaattctgcc 91380
ataaccccga ccccagtaac ccctacgaga aaaaggaaga tcctcgcttt attcacgagt 91440
acatcatgga tccagactgc caaaacgcat tcttcagcat actcgtctat ttttgggaga 91500
agctacagaa ggaatacaac gggcagatta aaaaagtgtt ttgtcccacc attgagagcg 91560
aaacggaggc gtacagaaag tcacaagata cgctacatag gtttatcaca gaaagagtcg 91620
tggagtcgcc ctccgcagaa actgtgtaca acctatccga ggtcgtgacg gcctacgcgg 91680
aatggtacaa caccaacatt aacgtaaagc gccatattgc cctcgagcta tcccaggagt 91740
tagaaaactc tgtgctagaa aaataccttc agtggtctcc caacaaaacg cgaattctaa 91800
agggttgccg tattttgcat aaatttgaaa cgctgcagcc cggcgaatcc tacattgggg 91860
tgtccacggc cggcacactc ctaaacacac ccatatgcga gccaaaaaat aaatggtggg 91920
aatggtcccc taatccctct gcccctcctg agaaagaagc gtctgcacca actccttagg 91980
gaatatcctt agaagcatgt ctttcggcag agccattacc ggtagcaaaa aagcaacatt 92040
gagtatatta tatgccttag cctgctcata agcgtccttt tttttcatgg tattttatgt 92100
ttttaaatat ttttaattat tttttaaata cgatgaacag ttcgtgctcc gaaggctgtt 92160
tactaaaaat cggtgtgaat ccgcattctt taaatatggt ttcccattcg gggatggtat 92220
ggaaatccat gtctctacga atagtatggt gcccaagtgc gtcctgcagg ctgtgaagcc 92280
agaaggcctc ctgaccttga tgaaggtcgt acatgataag aaaaccatca ggtttcaaca 92340
gatggtaaag cttgttaaaa tcgtttatcg taagatgatg cgccgccata ggtaacccta 92400
tgagctccac agagttttca tgctggacat cgtccatatc ggtataaaac gtttcacagt 92460
aaatgagacg cttaaacgag tatcgatgac aaacatttat ttccaagtag gtttgcacta 92520
cgtttttagg tatatcggga atcatgttga ttaaggttgt ttcgggaaac ttaatcatct 92580
gactaggctt cattttcaac tctttaaagg atttcccgga gaagtgaaaa tgggtcttta 92640
cgtatttatg taaaaatacc tgaatgggca gagggggctc ctcctcttcg ttctcgacgc 92700
ctcccaaaat atttggaatt tcctgacgtg gcaaaagaaa gtttatgtcc acgtttacga 92760
atccatcgag gacggacaca aagcttggct ctaatctcca ttccatatac tgtttagaaa 92820
cgggagatag cataatccta ggcgtcacaa tgcacgaagg gtttttaatc accgcatcgt 92880
ggtaagaaaa gtgtattcca tttcttccag tataaagaag cctatgttcg tcgtagcaga 92940
aacaattaag gcggtatgcc tcatacatac actgtttcaa agtacaaaca cgttttaaaa 93000
aggtttctgc attggcggag gccaagcggt tttgccattg gtggaagggg ttcaatccta 93060
caatggccag ctcgtttaaa atatcttcgc ggcgcgctaa aatctgcacc atagaagaat 93120
actttagcat ttttttttcg caccattcgc gaagatgttt agctacatta ttaaccttat 93180
tattgataaa gtatacgatg gcatgttgga agccttcaaa aataaagagc ccctccaaaa 93240
gatcatctgc caatagaaga tggatgttgg tgtaagcatt gtcaatattt tgtagaaacg 93300
gcggaatgcc tgccaaaacc gcttcagcaa gcatagctcc gttccgttgt ttactgtcca 93360
atagattcgt aagttttttg tccgcaacag acacgacggc taggatggtt gcaatgtcag 93420
aaatggcggc ttgccagaaa taacccgaaa agcacatgcg cgcttcttct atagataaaa 93480
acgaaaagcg agaggcaatg tctccgagct gcgtgagttg aagacctttt tctcctctgg 93540
ttaaaaggcc tgccacaatg gcccgctcaa tggctgatgc cagcgcatcc gtggggggag 93600
gatccagcat atcaatctcc tctgccttaa acacgccttc cttatttttt ttaatcgttt 93660
ctacgacaat gctaagaaaa atggccccag ggccttccgt aatgatttca ggatactgct 93720
gcactggtat ttgctcaaag acgtgttttg tgtaaagcgg gtaaaagtgc ccaggaaata 93780
ctctccctac acgccccttt ctttgctcga tacggctttg agccgcgggg cgcgtaataa 93840
gccctcccgc ccattcggga tagtaggttt caatgcttct gttccacccg ggatctatga 93900
cgtacttcag cgtttcaatg gtaaggcccg tttccgcaac aaccgtggaa acaatgaccc 93960
ttcttaaagg tttttccact ttagcggtta agggattttt cacccacaga ttcttaattt 94020
ccgctttcag gccaaggtag gcctcatttt cctgcgcaat cgcctcacta tcgatcggca 94080
aaatcaacat taacggcagc ttttctttgg caaggtccat atttgcatta ttcagcaaca 94140
tcgaaaggaa gcgtatttca gccataccgg gcatgaaaat taaaatatct gcttccgtgg 94200
gacgatcatg aatgttttct ttatgaatag tgagagccgt ttcgcaggcg gtcttaatgt 94260
agttgttggt gttatacagc ggccagtggg tttccacacc gtactgtcgt ccttccacca 94320
aaataatgtt ttcttttccg ataccaaaat aggttgagta tttatgggta tcaatggtgg 94380
cggaggttaa aattacaaag ggaatacgca gcgcccctat gcttcctctt tgcaacatgc 94440
gctgaagcat acttttaata tacatgagca taaggtcgat gcctagggct cgctcatggg 94500
cctcatctat aatcataaag gcatagcggg aagctatctc atcatccgtc attgtatgta 94560
gctgcgccaa cagaaccccc gcggttgcat aaataaggcc ccgattgggt ttttccgtca 94620
gaggcttcgt ttggtagccc actgtttggc ctaatatcat gtcggggtag tgggttgagg 94680
cgccgatgtc tttggcgagg gtcaccgcgg ttaggactct tggctgggta caaataaccg 94740
agcgtcccaa gtatttttgg aaagaatgcg tgttttcatt tctcagaatt ctgaacacgt 94800
gtacgggtaa ggccgtggat tttccggaac cagtgcgtga ctttataatg agcacccggt 94860
ctgcgaggga ggttggaatg gcccctccaa actccgggag acgttgtttt atccaagtga 94920
tgatgtaatg aataggaaca tcattcttgt gctcagcggg cacgttatag agatgaccag 94980
gctccaataa agtcggtttt cccatattct attgttttaa ggattgattg ttcataaata 95040
tttttatact ctgaccaaga aattattttt ttattaagcc ggttatttac gttgttatgg 95100
aacgcgaagg tccagtactg aaagtcctcc gagttgttta atgtcaaggg attttttgta 95160
agatacgaaa aggcgtggtg ctggcacctg gtgcatggca gagactcgat aaagttcagt 95220
atccattgga tggcttcata tttttctttc cagctaggag cgtctgaaaa aaagatagca 95280
tatagatgca aggatcgcca gtatttaggt ccccaatgca acatttataa ccttttgaaa 95340
aatctcattc catatagagg taaatatttt ttttccatgg agaatttttt tgcactcttg 95400
aagggattgc gccacatcgt caaatgtttt ttgttttcca tgtattttgg cgtaattcca 95460
gccagtatct gtgtcatggt ccttaatgtc atccgctaac tgaaaggcat gtccaaaaca 95520
atgggcagcc ctttcaatca tcccaatgtc ttcaacggat ccagttccta aaacccagcc 95580
cataataaac gcgatcttaa aaaagggaat ggttttttct ggagtgtcta ctaactgacc 95640
ggaacccgcg ctgtttagag agtggcttac aaaggtacac agcagcgctc ccagttggtt 95700
gggatccgga aaccttggac agtgttcctt aatccagtcg atttgccggc aaatattttg 95760
aaatccttgc atggttagcg ccagagcgct catctgcgcc ttggctacgc caaagcgggc 95820
ccacactgta tctttatttc gccgcttcac atcgttgtca aaggagggca tatcatcgat 95880
aatcaaagaa gctacgtgaa agtactccgc tgctagggcg gcctctgccg gataaatagg 95940
cgccccaaag gaatgttgca actgacaggc ccgaacaatt tccatcagga taatgggacg 96000
gatatacttc ccacctctta gagcgtaaga gcaaggctct gttagttgtc ccttaaagtc 96060
cccatcttca atagcattat ttaagatggt ctcaaactct tcactaaagg ttttataatt 96120
tttaggattc agtggatgta ttccatgaaa aagcgcgaca ctacgcggtg ctgtgattct 96180
aaaatactta ggtttgcgcg tataggatat taaaataata ataagaacta caatgatgga 96240
gatatagatg agatgcaaca tgctgagttg tctccccgca gggaatggtc cttttccgcg 96300
cttgttaacg gtaccgagga ggcgttgaaa tctttaggaa aggtgctgtc tagtttggaa 96360
tctccaattc ctcccgtata tttaggtata taattattgt gtctagaaat tgtttgcttt 96420
gaggtatcaa aatattcagc ctgaccgcta tttcttttag aataattcgg tatagggctt 96480
gagtagttgg caatactctt aaaccggggc accaaggtaa caatattttc catataatgg 96540
gtttgatacg ctttgtttaa aaatgggctt accggcttta tgcttgttag ttgtgcattg 96600
agtaccggta tgtcttctag gatttgtggc tttatagaat gattagcaaa cacagaatgt 96660
agtatattag atacttgtag catatgtcta tttgcggaaa attcctggta ttctctgccg 96720
tgttgcgaat ctttgggcgg aaggggacca agcatcggca cgtccgtgta ggtactggtg 96780
gattttatga gttcctgctc tatgttcggt ttgacatgtg gatttcctaa aggaatacct 96840
ctacctgcaa tccctttttc taccgacgca ggtagattgt gcgctaaaca caaaatattg 96900
tacacgtctt tgtgcggaat atatccgtta tagtgctggc ccggcatctg atcgccaagg 96960
tgctgctcat gcttaatggt accctttgtt ctgagtttag gaagatcctc gtacgaaaaa 97020
aattttgtgt gctcgctgaa cctcgtagaa ggaaccgaac tattttttgg gttttttaag 97080
gaaggcaatg aggaaggctg ggtcagacaa tttttctgtg tgccctttaa gctagccacc 97140
tgcggaaatg tttttttttc cgtacgaaca acattgcgcc taattaggtt ttccgtatgg 97200
gttgaaaaag caggacgatg atttttaaaa tgattaaaaa gtttattttt tggaatggag 97260
ctgtacggct ccagatcttg cgcatcgccg taaccaatgt ttttgtgctg agggttcagc 97320
ataaaagaaa agttacgtag atcactgagt tgcaatccct tttcagcctt ttcaggacta 97380
ttagtgtatt cattgtatac aggcgcagct ccatttttgt tgccgcagta ccgggaattt 97440
agtatattat cagaataccg gttatgacgc ggcaaatcgc tttcccaaag aggtggatct 97500
gacctataat cggctaacag ctttgaagca taatcatgat acattgtata taaaagttaa 97560
ttattatatt gagaaggcat aattacttct tgtaggggta caagaggctt tgaatcaggc 97620
aaactgacgg gttttgaatc ggccggcttt ggaccggcag gtatcttttt aggttgatct 97680
tcttctagct cattagacac ggatggggga gaaataggag gaataatttc atctccgccc 97740
ttatatttgt catggataga agaaacaatt acatccatgt ttgatttatt ataaatgtcg 97800
tttaactggt gatttaaaac ataataatgc aaaaataata gggctacaat gcatatatat 97860
acgtaaatag ccgtcttcgt ttttcgtttt ttatccaccg gcggattaca aattgcaaaa 97920
aatacaacta ataccaccgc tgtaatgatt aaggccacaa tgaaaggatt ttgaaaggat 97980
gttttgaacg gttcgcacgt ataaattttt tctcctaaat tattgatacc cgcaataaaa 98040
tctacattca ttttatatat ttataaatta tgaaaaattt agagttacat ctccgccgga 98100
ccaatcattg ctaaaatttg aagattcttc aaaaaggccc gactggttga atgtcttctg 98160
ctcaggtttc caaaaatttt ccaagaatgg attttgaaca ataggctcat cttgattttc 98220
ttcttcaagg atattttctt tgatatcaag aacagcttct ttaaactcag gtgtatcttg 98280
attaaactca ggtttatcct gatcaatcgc aaaaatatta tcttcttcag atatatcctg 98340
tttaatcgca agaatagttt cttcctcagg tttatcctga tcaatcgcaa gaatattttc 98400
ttcttcaggt ttatcctgac caaactcaac aatatctttc tcgctaaatc cgtttttagt 98460
gtgaagctct tggttttgaa gagaattatc aaaatctatt ttagttgttg tcctagaccg 98520
tggcacggga tagttatcta atggtttact tactatagtc ctcgaatgtg gcacgggata 98580
attgtttggt gacttgctgg ttagctcttg gcttgttaat agttcttgtt ttctcaataa 98640
ttccatctct actacttctt tttgatccgc tggtgtctct ttttggtatt cttcattaga 98700
aaaatgttca gagggtaatg tttcaataaa ctttgtgagt ggatagctgc tctttgatgt 98760
agaagagcgt tgaatttgct gataaaggag ttgaacaagt cgccggtatt cactctgtct 98820
tttttcatat tttttacgta gcgtggagag atctgctaag agcgacttgt tttcagatgt 98880
taattcttca atttgatgaa gaaggctgcg attgtatgaa ctaagtcttg catacgtttc 98940
ttctaattct gtctccggct ccacataggc ctgttttcgc agaaatttat tgtatagttc 99000
cattcttttt ttgagcagaa aggtaagact ataatcttgc atttctttcg taactttatg 99060
gtagttttct ttccggtttt tgataataaa gggcagcatt ttttctgttg tgataaaggt 99120
gcccagattg ctaatgtagt cgcacagtag caattccaag atagattctt tcttttcaag 99180
gcttatagat tggctgtatt ctttaggtat gaaagaatca acaatcgttg ttacgaagtt 99240
tgaaaagttt aatgttttgc tgttaatttg ggtaatgtta caaaaatatt tgtaaaaact 99300
atctagcatt ttttcataaa gttttttatt ttgtttaacc cctaaaatat agccctttac 99360
ttgatactga tattccgtaa caatggaatg ttttttgtat agtgcatttt tgtataaaaa 99420
gttataaaaa atgttgataa aatacgcacc aagggtttca aaaatactta taacgtggga 99480
ttcttcctga tccattatat catatgtaat attattttaa taaaaaatta ctgacgaata 99540
acatgcaaaa aaaatatgtt taaacttatt ttaagctagc acttatttaa aagtgtttta 99600
aacacgtttt aaattgtatg ttaatacact taaaaattaa gccgaaattt gctccaataa 99660
ggattacttt tatcaatgac cacctcttta ctataaacgg ctttacataa ttttaataat 99720
gctttagagc caaagctgaa ggcagtggga agcggcactg tactatggta aaaatgttgc 99780
cgatgttcat cctcgcggat gtacacaagt ttcctatatc ctttaaacac aatatggcta 99840
atttcttcca catactcctt atcctgtttg gaatagcggt tgctttgacg ggaaaaattc 99900
gacatacaaa tagaggcatt tgtaaaaatg gaaacaaatg cgtttttacg aagattggcg 99960
ggtaaatcgg tatcatcttg gcagcaaata atcatcgaaa taaaacagtg acgattttgg 100020
taaaaaaact ttttaaaaat ttcttttgta aataatgggt gcagttcggc cgcgcagtcg 100080
tctaatatta aaagtaaacg aggattaaga ttgatatagt ttaacgtaaa cttttcatcc 100140
tctgtaaggc ataagttttt atacatatga atgttctgta taataatttt ttttaaaagt 100200
tgctgataaa gcgatgtaat cttttcttct tttttttggt ccgtttgttc agcctttaag 100260
cactccactt ttgcaatatt tttgttttcc ttttgctgta tatcgatcgg aagtttatga 100320
tacaatgttt ttagcatatc gatgttgttt actcgactgt agatggagga catcatagtt 100380
tgccgctgcc agatggcctc caaaaagcgt tcagcgccct tgttgtcatt ttttttttgc 100440
ttatcggcga gccacaagcg gtagtgtatt agagttggat gtacaaaacc ctcatatgaa 100500
cgatttgagg gttccgaggg ggcaaccact aaaatttgtt caatatgggg ttgcaggatt 100560
ttcataatat gtttaacgta cacggttttg cctgtttttg aggggccata tagcacagtt 100620
gttttatcta taaaatgatg tgctttgaac tgtagttcag gaattagctt ccctgaatgg 100680
gtcgttaggg ccatctctat attattacaa ttctgctttt gtatataaaa tttctttttc 100740
gagtttatta ttattgttga cccacatatc tacccgtatc gtatcatcag gcacattgag 100800
catttcaagc gcattatcta actgtttttt tgtttttatc agctcgcttt cttcatcggg 100860
ggttaaattt tctttactaa gcagttgctt aattttttct tcgcagtcgt ctataaaatc 100920
atactctcga gcttttttga tatttccaga tgctttttct aggtttttta gctccttaaa 100980
ggaaagcagt cccttaatcc cgctatccgt gtgaaaggtt gaattataga tggagagccc 101040
cggagcatcc gggccagttt cttgtatatt ttttgctttt ttgtggtaaa tagtatttcg 101100
taaaatctct tttcctatct ttaggtcttc ctcatgacgg tccaaaatcc gttttattat 101160
ttcattattt tgattaaaat aattgtagcg ctctctgttg gccttaaagc ttcccaggag 101220
tgtccagttg cctaattgaa tggatgaaac ctctgagaaa atctggtctt tatatttata 101280
ataaaattca tcaacctttt gttggttgct gctatccacc acatcataaa taatgaaggc 101340
aaactctagg tcgggttttt ctgggtagat gctttccgta gcggcccgca actcttcgta 101400
attatcctca atgtaataat tccacttata aaaagtatcc tgaggtggaa tatgctgcga 101460
aagatatcta gtaatttttg tgttaaagag aatgggttta aacgccctcg gattttcaag 101520
catatgttta atgctttggt gaagttctat attttgtaat atgtgggctg ctgccctata 101580
gccctgtggg gtttgggtga ttgcatcaat atcggcctga agctcattag gcacatttaa 101640
tgttttttgc atgatgtgta aagggatgcg ctcaggatct gctaaatcgg tgtattctgt 101700
gcttgtacaa gtgcttgcac aggtatctac attggtatct gcacacatgc ttgcacaggt 101760
gtctacattg gtatctgcac acatgcttgc acaagtgtct acattggtat ctgcacaagt 101820
atacgcactt tgagcatgaa gattaggatc aaacacaaaa tgttctcgta aaaagctatc 101880
gatcgttgtt ttagcttcct tgcttttctg cgtctgggtt ttgcagctat ctgctataga 101940
taaaattgta tttactaccg attcagaggg aacatcatta gtttcctgtt tcaaagtatc 102000
aactaacgtt attagctcac tgagaagagt tttggtcgtg tgggtaggtt ttgaatagga 102060
aggcatccat tcctgcagag ctttgaagac atatccaata aagctagtca ttataagacg 102120
tcgaatatac tgctcccgca aatttgtaaa agagcaaaag gccaccctgc tatcattttt 102180
gaactgtttg taagggttcg tcctttggta aagctgttta agcgtttctt cggatatttc 102240
agtagaggga tcctccaata cgtttttgag aagctcatca atattaaatt ctgccatatc 102300
ttagagttta ttatatacat attaaagctt taatataagg gggggtataa caatggacga 102360
aatcatcaat aaataccaag ctgttgaaaa actttttaag gaaattcagc aaggattggc 102420
cgcgtatgat caatacaaga ccttaattag tgaaatgatg cactataata atcatatcaa 102480
gcaggagtat tttaactttt taatgattat ttcaccttat cttattaggg cgcatagcgg 102540
agaaacgctg cgaaacaaag taaataatga aattaaacgt cttattttgg ttgaaaatat 102600
caataccaaa atatctaaaa cgctggtaag tgttaatttt ttactacaga aaaaactttc 102660
aacggacggg gtgaaaacga aaaacatgtg gtgcaccaat aatcccatgc tgcaggtaaa 102720
aacagcccac aaccttttta agcaactatg cgacacacag tccaaaactc aatgggtaca 102780
aactttaaaa tataaggaat gcaagtattg tcataccgac atggtgttta acaccacgca 102840
gtttgggctg caatgtccta actgcggttg tattcaagaa ttgatgggaa ccatttttga 102900
tgaaacacat ttttacaacc atgatgggca gaaagcaaag tcaggtatct ttaaccctaa 102960
ccgtcactat cggttttgga tagaacatat tcttggtaga aatccagaac aagagttggg 103020
gaccaaacaa gatccctgcg gaaccaaggt gttgcaacaa ctaaaaaaaa ttattaagcg 103080
cgataataaa tgcatcgcgc ttttgacggt cgaaaatatt cgaaaaatgt taaaagagat 103140
aaaccgcaca gacttaaata attgtgtttc tcttatattg cgtaaactta ccggagtagg 103200
gccgcctcaa atatcagagt cgattttact acgaggcgaa tacatattta cagaggcaat 103260
taagatacgg gaaaaagtgt gtaaaaaagg gcgtattaat aggaattatt atccgtatta 103320
tatatataaa atttttgacg ccattttgcc tccaaatgat accacgaatc gacgcatttt 103380
acaatatatt catttgcaag gaaatgatac gctagctaat aatgatagtg agtgggaatc 103440
tatctgtatg gagctccctg aaataaaatg gaagcccaca gatcgaaccc attgtgttca 103500
ttttttttaa agatgaagat tttttagatg atttttttta gttttttaaa agacgaaaaa 103560
attttttaaa agatgaatat tcttaaaccc cgcaaattac ttttttttag gtactgtaac 103620
gcagcacagc tgaaccgttc tgaagaagaa gaaagttaat agcagatgcc gataccacaa 103680
gatcagccgt agtgatagac cccacgtaat ccgtgtccca actaatataa aattctcttg 103740
ctctggatac gttaatatga ccactgggtt ggtattcctc ccgtggcttc aaagcaaagg 103800
taatcatcat cgcacccgga tcatcggggg ttttaatcgc attgcctccg tagtggaagg 103860
gtatgtaaga gctgcagaac tttgatggaa atttatcgat aagattgata ccatgagcag 103920
ttacggaaat gtttttaata ataggtaatg tgatcggata cgtaacgggg ctaatatcag 103980
atatagatga acatgcgtct ggaagagctg tatctctatc ctgaaagctt atctctgcgt 104040
ggtgagtggg ctgcataatg gcgttaacaa catgtccgaa cttgtgccaa tctcggtgtt 104100
gatgaggatt ttgatcggag atgttccagg taggttttaa tcctataaac atatattcaa 104160
tgggccattt aagagcagac attagttttt catcgtggtg gttattgttg gtgtgggtca 104220
cctgcgtttt atggacacgt atcagcgaaa agcgaacgcg ttttacaaaa aggttgtgta 104280
tttcaggggt tacaaacagg ttattgatgt aaagttcatt attcgtgagc gagatttcat 104340
taatgactcc tgggataaac catggtttaa agcgtatatt gcgtctactg gggcgtccag 104400
ctataaaacg tgactggcgt acaaaaagtc caggaaattc attcaccaaa tccttttgcg 104460
atgcaagctt tatggtgata aagcgctcgc cgaagggaat ggatactgag ggaatagcaa 104520
ggttcacgtt ctcattaaac caaaagcgca acttaatcca gagcgcaaga gggggctgat 104580
agtatttagg ggtttgaggt ccattacagc tgtaatgaac attacgtctt atgtccagat 104640
acgttgcgtc cgtgatagga gtaatatctt gtttacctgc tgtttggata ttgtgagagt 104700
tctcgggaaa atgctgtgaa agaaatttcg ggttggtatg gctacacgtt cgctgcgtat 104760
cattttcatc ggtaagaata ggtttgcttt ggtgcggctt gtgcaaatca tgaatgttgc 104820
ataggagagg gccactggtt ccctccaccg atacctcctg gccaaccaag tgcttatatc 104880
cagtcatttt atcccctggg atgcaaaatt tgcgcacaag cgttgtgaca tccgaactat 104940
attcgtctag ggaatttcca tttacatcga atcttacgtt ttcataaagt cgttctccgg 105000
ggtattcgca gtagtaaacc aagtttcggt acgcattctt tgtgccgggt acaatgggtc 105060
ttccaaaagg atctacaagc gtgtaaacgg cgccctctaa gggtgtttgg ttgtcccagt 105120
catatccgtt gcgaggaaac gtttgaagct gcccatgggc ccccatctgg gacgtgccct 105180
gaatcggagc atcctgccag gatgaatgac atgcacccaa tatatgatgg cccaccatat 105240
catggaaaaa gtctccgtac tggggaatac caaaggtaag cttgtttccc aaggtggggg 105300
tacccgtatg cgggcgtact ttattgtatt caaaccctac tggaacataa ggcttaaaat 105360
gcgcattaaa atgcaccaaa tgtgtttctt cgatttgact caaagtgggt tcgggatcgg 105420
gtttcccata acttttgttc acatttttaa tgttagagat cctgctattc agcaagtctt 105480
gggccaatat aatcttgtcg gccttcccat cgttagcaat aagacaaaaa gctcctcctg 105540
atgccatata taatgttata aaaataattt attgttttta ttaaatatgg cggtttatgc 105600
gaaggatctt gataataaca aagagttaaa ccaaaaatta attaacgatc agcttaaaat 105660
tattgacacg ctcttgctgg cagaaaaaaa aaactttttg gtgtatgaac tacctgcccc 105720
ttttgacttt tcctccggcg accctttggc cagtcagcgc gacatatact atgccatcat 105780
aaaaagcctc gaggagcgcg ggtttactgt caaaatatgt atgaaagggg atcgtgccct 105840
ccttttcatc acctggaaaa aaatacaatc cattgagata aacaaaaaag aagaatatct 105900
gcgcatgcac ttcatacaag acgaagagaa agcattttat tgtaaatttt tagagtctag 105960
atgagctttt acgcaatgtt gtacagtgtt gtatatatgt cttgtaagca tttgttgtag 106020
agtaataagt aaaagataaa taaaaatgac tattaaaata aagcccaaac cattaaaaat 106080
atttttatct gttagattta atttaataaa tggctcatgg aatgtgtggt gcgccgctgc 106140
atgaggtgtg gccgcatggg atgtggtcgc ataagatgta gctacatggg atgtggcatt 106200
tgcttgcatg taaggatcat gatgtgttgg gtcttcatcc cagcaataat cgccatcttt 106260
atctagctga attgtatacc ccattatata tcacttatta ttttttttta atgtttcatg 106320
aatttcatta taggcggtga aagggtcctc aggccccttc tgtaaaagat tatagagatc 106380
ttcggacgct ttatgtttcg tgcgaattaa ggcgggatat aacaaaagag agggccccag 106440
ttccaaacaa attttactta gcgggctcat attttgcacc aagtttccca ctacttgcga 106500
tgtttcataa cgcattttaa agagctttat cataaaagtg ttatgcaggc cggtgtagtc 106560
tggcctatag ttaaggaagg ggatttctct ggtaccgtca aacacgatct caagtcctct 106620
agcaagcccg atcaaaattt cttcagcaat ggatgagtat ctaattccta cattacgaag 106680
cgtaagcatt tctataacat catctatttc ctgcatagag gaatctattg taggaatttt 106740
aatatcatct gtgctgattt gttcattccc aagataggta agcagcatat taattttttc 106800
tagctttact agcttagtct tacgctcata atcatgatct tttttataaa aagagttggg 106860
atcaccgttg gaccgtagat gattaataag gcggtctact tgctttgtac taggtttaat 106920
acttttttca ctatactcgc tttcagcata gtggttttta cgatctcttt tagaaatagc 106980
tgttttttga gatgcctcag actctgcata tttttttcta tgcgtagaaa gagaataacc 107040
gcggtcatta cgtgaactac tgttgcatgc aaggcctcgg cgcgtcttac cgctgcgcac 107100
actgccattg cgtatactgc catcgcgcac actgccgctg cgtatactgc cattgcgtat 107160
actgccgctg cgtatgctgc cgctgcgtat gctgccgcta catacactat cactacatat 107220
gctgtcagta catacgctat cgcggcgtat gccgccgtgt accttatcgc cgcccctacc 107280
cgagggtttt ttagatataa tactgtgtgg ggagtcaagc gaaaattcag ggtcattaaa 107340
gttaatgccc aatgactttg ccaatccatt aagctcttca tcaaaatgat cggtaggaaa 107400
actttgttgc ttgcccatga cctgtttttc aagttcctcc aaattggctt gctcatttat 107460
atggagatta ttcataagcg tcgtaattcc agcaagattt gctccttcta aaaatgtggt 107520
gtcctccatc ggatatacta tactatttaa aagcttttaa ataaaaatgt gtttggaaga 107580
aatgctctct tcaagcgtgt gtagctcaga tataaatgcc tcctcagaaa gctttccacc 107640
atactccttt ctcatcgtat aggagggcgc cggtttaatg taggaaatcc actgggaggt 107700
aaaaaaccgg tacaacatat ttagcagctc gcgggcctcc caccttttgg gctccgtata 107760
gtgcacatca acataagagg cggcgcatga aaagctgcaa aagttgccga gaacgcccat 107820
ctcaatctct cctcgctcat tttcacgcat ataggtgggc acgaattttg ggacagtctt 107880
gaaatagaga tgacatgtcc agcatttaaa gctagaatgg gtaacccatt tggaaacagt 107940
ggtgaatacg gagggtagct ttttttcgac ctcggcttca tcgtcattcg tatttaacgt 108000
atcggtggca gtttttttgg attgcaagca ttcttcaatg gtaatcccgg ataagtataa 108060
aatattagga caattagttt ccataatttt gatagttatt tttatacaac atggatttaa 108120
ttaaagataa atggaggacg aaacggaact gtgttttcgg tcaaacaagg tgacgaggct 108180
tgaaatgttt gtctgcacat acgggggaaa aattaccagc cttgcatgtt cgcatatgga 108240
gttaattaaa atgttgcaaa ttgctgagcc ggtgaaggca ttgaactgca actttggcca 108300
ccagtgccta ccgggctacg aatctttaat aaagactccg aaaaaaacta aaaacatgtt 108360
gcgccgtccg cgcaaaacag aaggcgatgg gacttgcttc aatagtgcca ttgaagcctc 108420
cattttgttt aaggacaaga tgtataaatt aaaatgtttt cctagtaccg gggaaattca 108480
ggtcccgggc gtcatttttc cggattttga agacggaaaa aacattatac agcagtgggt 108540
agacttcttg caacatcaac ccattgaaaa aaaaatccag attattgaat ttaaaacgat 108600
tatgattaat tttaagtttc aaataaaccc agtgtctccc cgcgtcatca ttcatttaaa 108660
aaaatttgca gctttgttgg aacacatccc tactccatat cccatacgtg aaataaagcc 108720
tccattagaa gactcaaaag tatccgcaaa atttatggtc agtccgggaa aaaaagtacg 108780
cattaatgtt tttcttaaag gtaagataaa tattttaggc tgcaacacaa aggaatccgc 108840
ggagaccatt tatacgtttt tgaaagatct tatcagcgta cattggcaag aaattttgtg 108900
cgtgttaccg gtacccgatt aaagaatgtt ttcattaata aggtaatcga ctatgctaaa 108960
aagaataaca agaaaaatac cttgaagaac tataccaaag taggtaggtt ttctgcatgt 109020
cacggcatgg ttaaaattgc taataatgta gtccacaaaa gcattgctca atacgactaa 109080
aaatagtaaa aaaaggataa gtgctctttt tatatccata tactttaaaa cttatttttt 109140
acactaataa tttcctgcgg ccgcaatata aactgtaggt catctataac gcccagacct 109200
gttaaaagta gagtactatg ttttaaggga tttaaaatat ccgccgcaag aatgtgaata 109260
taattttcaa agtggtttac aggaatgcgt aagcgttttt ttttgcactg cggttggttt 109320
agggtcgaat actggcagga ggtatatata ttaataagac cgcggtcgat ggtttcaata 109380
tcttcataga attcaatgcg cggcgtcaaa agttttttaa gatgttgaca taactcatca 109440
tacgtgtagg actggagggg ggaaagaagg gtgtagtcaa agttaaaaat gtttttttga 109500
agaaccttta aagcatgttc cgcgtccgtg gtttccaaaa tatgttttat ggtatgaatg 109560
tcatttaaat ctacaaagtc tgacagcttt gtgtagaact cggtgacgga ggttattttc 109620
tggaaatcgg ttttttgaaa aagattttca atgtgtttgc gggttgagtt gctttgcagt 109680
ccatacaaga catcaaaaaa ttcaatcagc aaaaacttat acaaatggtt aatataaaaa 109740
gctttgttgg ccttattctg ctgaggatat ggttcctcta ggggatatag aatggcttgg 109800
tctatatccc taggatcaat agtcaatgtt gcgatgggaa gcttttccag cgtagcggga 109860
agagtttggg ttggagcgta gtaaaagtat agcccggttt ttccctctga aagaaagccc 109920
acaaattctt tttttatatt ttgcagcacc gctgagggta cgatttcgta ctgtttatac 109980
tgtttgttga aaagggtaat aaatttccag gtttcttcaa agcttgcaat ctgggtgggc 110040
cgcagatcaa agtcgatggg aatgtcgtca tgaatgtagg atgatagtct tataggaaaa 110100
taaatagggc gatcggtgtc tgaatcgata agtaaagcat aacaaaagtt atgcctgttg 110160
ataagttttt taccaaccgt gtagccggga atgtttttca cgtcatggat atcccaccag 110220
ttatccttgc acataaactc gctcatagac tggatgacct ccatcacagg gtcatcttcg 110280
gtaaaaatat actgggcctc actgtttttc agaaatcttt tttgctgggt gatggccatt 110340
gggtagatcc cttcgtccgt gtcaaagata atggctatct tcttcgatgg gctaagaatt 110400
ttttgtattg tgctggggga cacctcaaac ccgatgtcgc cctgtttatc tttaaaaaag 110460
acacagtgaa ggtcgtagca tatggcaaca aggtccagaa agatgtcctg ccatgtggtg 110520
tcccattgaa gcagttggtt tttttgttca acaaaggttt gtaagataag gtttgccagc 110580
tccgcgccgc tggaaaacat gttgccggcc ccattcccca aaatatagta ctgcggtgtg 110640
ttggccgcct ttgcaatttc aatggcaagg gccttggggg caagatccaa aattcgagca 110700
agggaataaa aaagcccggc attgctaatt ccaagcatgg tttgctccac ccccacaatg 110760
caaaaaatgt cgggctcttt tatcgtattt aaaaacagtt catctgctat ctggtggggt 110820
agaaaggcaa tccggttcac cggtattttt tttccatagg acaaggtatg acgcgatgtt 110880
tgtgtattaa gatcctccag gtcttgttct acaaacgtgt gcttggtgag gcaggtattg 110940
ttaatataga accgctttgt gcccagcagg gccttcgtct tttggcagca cggcagacag 111000
taatttaggg ggtggcggcc ttctagtagg cttagatgag ggtagtcagg atgcgggcag 111060
ctatagtagg caggtacccc ctccgtgaaa ttccaatact ttactagctc cttgcgcttg 111120
gctggcggca tggacttcac ctcggcctct gagtaaatga cgggtggccg tgggtgctgg 111180
cataggacgg agtaaaccgt tgcctgcgtg tcgtacttgc gcaggtcata caggtcgggg 111240
tcctgttctt gaagcgcacg tagctgagag gctccctttc cttgttgttt atcgtgcagt 111300
tgagagagtt tattaaccaa aattttgtca ggcccggtga tcaagttatc taaaaacaca 111360
aataggtaaa cccaaagata gttaaactct tcctgggtaa tgttaaacat ttctattttg 111420
atatctgtaa ccctatggta gatgcgaatg ttgcggccgc cgtagattgt ttcccaccgg 111480
gccgcaacat ttgtgtcaaa gaggtacgca tacgtgtttt ggagcaacgc aacattgatg 111540
tccattttgc gccccggacc ggaggaaata atgatcatcc gttcgatttc gtggggatca 111600
tacgaataaa tccccttttt aaataaaaaa ttgtagaccc cggtttgctg gaggccccgc 111660
acggaaataa tccctgcttg ctcgtattcc cgccaacgac ttttgagctc ggtaaatccc 111720
ttgctagaaa gcgtataggg ccaaaaggtg gacaccgaca tggagctgat agaaatttgg 111780
atgtcctcgt tggagggaag gggcagactc cctccacgag gaaacgcggc aggccccata 111840
tcattaattg tatgaataat aggatttatg aaattattta gggtggacac cacggagtta 111900
aagtcgtggc gctcgttttc tgaccaattg ctttcgataa agtagtgccc attattttgt 111960
atggtaagaa taaaggcctt tttattgata aagcgtatta aaataatagt gggtacacgg 112020
aatgttttat tgctgaattt ttcaggctcc gtggaagtta tgtggtgttt ggaaaccacg 112080
gtgggacctg ttttactata aaagaacacc accagctgag gaatatcggg agtagctgga 112140
aataggtcga aaacattgcg cacattaatt tgaatattta cgaggggtga aattttaatc 112200
attgccgagg tgacggccaa cgtgccgcgt gttagtctat tcccctcgta cttggcaatg 112260
acttgttgtg ctctggcata cgtaaagttt attagttttt gctctaggag aagcctcttt 112320
ttaagactgg tcaaggatgg agaaagagca ggatactgtt tttccatttg taagggagat 112380
tgtaccaata gtttaaaggc atcgggggaa agaagaggcc aatacttcat aataaggccg 112440
taatagagta agtcaaattg gtaattatcc tctatggcaa tggagatttg gcgccgcatg 112500
ggggccacta gcgtgttgag gtctgctaca aagatgtgat gaatgttttt tatgagctgg 112560
aagctgtcga gcgcttccac atagagctca tctttttgac tttccataga tgcgtcgatg 112620
ttcaccccac ccacctgttg aaactccttt ttgtagtcgc gaatgtctaa cgccaccccg 112680
ctaccgctta acaataggcg atacgttacc tgaagcgcat tgttttgaaa aaagaaaatg 112740
tgttgtctat aaggggggat ccctgtggca acgtaaattt tttctcgaat gtctttaaaa 112800
gtgtcttcag ggaaaatact atactcgcta tacatcgtct caatttctgg catcatcacg 112860
tttgtctcct cgccacgatc ctccacaaaa agtttttcaa actcatctaa atcatcgcta 112920
tctccaccca ccacgtattg ggaaagcttt ttctcccaat cctcgccgta aaaattttgt 112980
aaaatttctt tgtccttagg ggttcgctgc aggtctttgc ggcaggcctg taacacgttt 113040
gcaggaacgg atcccaaaaa aataaacgtc ttcgtgtact cattttccac aggattataa 113100
agagtaactc gtagaggatt tgttaaaaag tcattttgga aatccattat acccggtata 113160
gaaaataaaa tttaaaataa aaaacggatg atatctatca tggaccgttc tgagattgtt 113220
gcacgggaga acccggtgat tacccaacga gttacaaatc tcctacaaac caatgctcct 113280
ctactattca tgcccattga tatccatgaa gtacgatatg gagcctacac acttttcatg 113340
tatggttccc tcgaaaacgg ttacaaagca gaagtaagga ttgaaaacat cccagttttc 113400
tttgacgtac agattgagtt caatgataca aaccagcttt ttttaaagtc gctactgacg 113460
gctgaaaata ttgtgtatga acggctggag acgctcaccc agcgtcctgt aatggggtac 113520
cgcgagaagg aaaaagagtt tgcaccatac attcgaatat tttttaaaag cctgtatgag 113580
cgacgaaaag ccattactta cttaaataat atgggctaca acacggccgc ggacgacaca 113640
acctgttatt accgaatggt ttcccgagaa ttaaaactac ctcttacaag ttggatacag 113700
cttcagcact attcctacga gcctcgcggc ttggtacaca ggttttccgt aacccccgag 113760
gatcttgttt cctatcagaa tgatggcccc acagaccaca gcatcgttat ggcctacgat 113820
atagagacct atagccctgt taagggaacc gttccggacc caaatcaggc aaacgacgtg 113880
gtgttcatga tatgcatgcg cattttttgg attcactcca cagagcctct agcgagcacg 113940
tgcatcacca tggcaccctg caaaaagtcc tcagagtgga ccaccattct atgctcctct 114000
gaaaaaaatt tgttgttaag ctttgctgaa cagtttagcc gctgggctcc tgatatatgc 114060
acagggttca atgattctcg gtacgactgg ccctttatcg ttgaaaaatc tatgcagcac 114120
ggtattctag aagaaatctt taacaaaatg agccttttct ggcaccaaaa gctggatacc 114180
attctaaaat gctattacgt aaaggaaaag agagtcaaaa tctcggccga aaaatcgatc 114240
atttcctcct ttttgcatac ccctggatgc ctacccattg atgtccgcaa catgtgtatg 114300
cagctttacc ctaaagccga aaaaacaagc ttgaaagcgt ttttagaaaa ttgtgggtta 114360
gattcgaagg tagacctgcc gtaccatctc atgtggaagt attatgaaac acgagacagc 114420
gaaaaaatag ccgacgtggc ctattactgc attatagatg cccagcgctg tcaggacctt 114480
ctggtgcgcc acaatgttat ccccgatcgc agagaggtag gaattctgtc atacacctcg 114540
ctgtatgact gtatctacta cgcgggagga cacaaggtat gcaatatgct cattgcctat 114600
gccatccatg atgaatacgg ccgtattgct tgcagtacca ttgcccgagg taagcgggaa 114660
cacggaaaat atcccggcgc ctttgtgata gaccccgtta aagggcttga acaggataaa 114720
cccaccacag gtctcgactt tgcgtcgctg tacccctcac tcatcatggc ctacaacttt 114780
tcgccagaaa aatttgtagc ctctcgggat gaggcaaata gcctcatggc caagggtgaa 114840
tctcttcact acgtctcctt tcactttaac aatcgtctcg tggaaggatg gtttgtgcgg 114900
cataataacg ttcctgataa aatgggattg tacccaaaag tactcatcga tctacttaac 114960
aaacggaccg cccttaaaca agagcttaaa aaactaggtg agaaaaaaga atgtatccat 115020
gaatcccatc ctgggtttaa ggaactacag tttcgccatg ccatggtaga cgcgaagcaa 115080
aaggcgttga aaattttcat gaacacgttt tacggcgagg caggtaacaa tttgtcgccc 115140
ttctttctgc ttcctctagc cggaggagtc accagttcgg gtcaatataa tcttaaactt 115200
gtctataact ttgttatcaa taaaggttac ggcatcaagt acggtgacac cgactcatta 115260
tacattacat gcccagatag tctttataca gaggtaacag acgcatattt aaacagccaa 115320
aaaacgataa aacattatga gcaactctgc cacgaaaaag tgcttctgtc tatgaaagcc 115380
atgtctacac tatgcgccga ggtgaatgaa tacctgcgac aagataatgg caccagttac 115440
ctacgtatgg cctacgagga agtactcttt cctgtgtgct ttacaggcaa gaaaaagtat 115500
tatggtattg ctcatgtaaa cacacccaat tttaatacaa aagaattatt catccgcgga 115560
atagatatca ttaagcaggg tcaaacaaaa ctcaccaaaa cgataggaac gcgaattatg 115620
gaagaatcca tgaaactacg ccgccctgag gaccatcgcc cccctcttat tgaaatcgtt 115680
aaaacggttt tgaaggatgc tgtggttaac atgaagcagt ggaattttga agacttcatc 115740
caaacagatg cgtggagacc ggacaaagac aacaaagcag tccaaatctt tatgtctcgc 115800
atgcacgctc ggcgtgagca actaaaaaaa cacggcgctg cagcatcgca atttgctgag 115860
cccgagccgg gagaacgctt ctcctacgtt atcgtggaaa aacaggtaca gtttgatatc 115920
cagggccacc gcacagattc ctccagaaag ggggacaaga tggaatacgt ctctgaagca 115980
aaggctaaaa atcttcctat tgatatattg ttttatatca acaactatgt tctaggcttg 116040
tgcgcgagat tcattaatga aaatgaagaa tttcaacccc ctgacaacgt cagcaataag 116100
gatgaatacg ctcagcgccg agctaaatcc tacctacaaa aattcgtgca atccattcac 116160
cctaaagaca agtctgtcat taagcaaggc aatgttcatc gacagtgcta caaatacatt 116220
caccaagaaa ttaaaaaaaa aataggcatc tttgccgacc tttataagga attttttaac 116280
aacaccacaa accccatcga aagctttatt caaagcactc agtttatgat acaatacttt 116340
gatggagaac aaaaagtaaa ccattctatg aaaaaaatgg ttgaacagca tgctacggct 116400
agtaatcgag ctggtaagcc cgctggtaat ccagccggca atgcgctgat gcgggctata 116460
tttacgcagc tgattacgga agaaaaaaaa attgtacaag ccttatacaa taagggggat 116520
gcaatacacg atcttctcac ctatatcatt aacaatataa attacaaaat tgccacgttt 116580
cagacgaaac agatgttgac gttcgagttt tccagtactc atgtagaact gctattaaag 116640
ctgaataaaa cgtggcttat tttggctgga attcatgtgg caaaaaaaca tctgcaagct 116700
tttttggatt catataacaa tgaatcgccg tctagaacat tcattcagca ggctatagag 116760
gaagaatgtg gcagtattaa accatcttgc tacgacttta tttcctaata cttcttaaga 116820
aactctttaa acaaggactt cgcatggtca aaggttctaa acccatggcc cttatgattc 116880
gccaaaaaag cggtttcatc aagattttct aaccctttca cggatgaaga aataaggtgt 116940
tcggcctcgt ttgcccattt tctatgattt tttttcacct cgggttctag atctgttttc 117000
tccatatact cattgtggtc atattttttt ttgggaggag gcgtgggtgg aggaatgggt 117060
ggaggaagta cacccgactt tcccgcttca accgttttat aaaaaaatag aagcataata 117120
caaagaataa ggactatcgc aaatatgata accagtgtcc cagtcgaggg cattttgtta 117180
tataagtaac gttttttttt attttttata attcgaatga agaaccatgt tgaatagtct 117240
tctactcaaa gacattttgt tatacggtaa atgagaattt ataaaatccg aatatcacta 117300
tcatactgtt tatctgagaa ggtctcactg ggtcctgtga tggagaaccc atactctgta 117360
atgctggggt ttataatgtg gtcaggactg acaagcacat ttctgaactg cgagagttct 117420
aggtttagac gcagtcgtaa tagtcgctgt atatttgtaa taaatattag attgcgtatg 117480
aggcgagtgt caaagcgatc ctttccaatt tgtactaagg tgggcttttg tattccaact 117540
cccacttgtt taacgatgga ccagggtcct tcttcccgat tttgttccgt gatataggtc 117600
agcacactat tttctgtata tgaggtatga tgtcgcatat taatacctgg tgccattcca 117660
actggcggtt gtgcaattcg ggctgtaccg ggacccaacc atcgtggagt tttataaaca 117720
tatcgttcta gcgtatttaa aaattcctta aggttattta cgagtagcat gaagggtgct 117780
attaaaacag gtggatggtt tataaccatt gtcataaacc attgcattgc ttcaatatca 117840
ttttgtaatg cttgacgggg aggcggggca ggtaatccac gtatgttgaa taaagcggtt 117900
aattgtgcac cggctgtttg gggcgtaata ttttgtatta aatttatcat cgaattggct 117960
tgcccggcat ttcctataag atcgattaaa ttggttattt gacctcgata ttgttgtacc 118020
cagttttgaa tggcagcgat gatctcaggg gttggattgt tttgaatttc aggtgtttgt 118080
attagattat tcacttctct tcgtgtatct tcaagctgag tcctaaatgc atttaactca 118140
cctataattt ggtttctatc aataacattt cttaaacctc gaactgtttc agccaatcgt 118200
atagtacgca caatttcatg taaggcctgg tttatgtata ttgacatggg atggccccac 118260
cgctcacgtc cacgttgaat acctgcggcc aaactaggac ctgcctcgtc ataatcaaat 118320
tgtgtaggat aaaggcttcc aaatagcact ttattgaaaa tttggtcaga aagaaattta 118380
gggcggccca tatttagcgc gttgtcccct ctaaagatgc gtgacatgta tccggcgttg 118440
cctttggata gtaactcatt cccatattga gtaatagaga ccgagacata ggggtttata 118500
agaagtttta gcataaattc tcgagtattt atggggggac gattcggaat gtttaatacc 118560
tctgcaacat ctggttgagg agccgtggtg tccagagatc gtactttttc agccgaaatg 118620
ccgtacataa gacaagcaat ttcttcaaaa ctatagtcat agttgtaaat attggcaagt 118680
ggtatagatc gcatcagcgc atttacattg ataggtataa tattcatatc aaacaagtta 118740
aatatgcgct cgcgctctct attagagcca agagtgcgtg tttgaccttt cggcgacact 118800
attttgtgaa tatgattgat ttgctcctct tggtaagagc tttccacgaa ggaaattacg 118860
tcttgcaatg ttttacgaag cgaatacact gcattcatcc ctattcccgc tgttataatg 118920
ggtttatcgt ctctgttctc gctaataaga ttaactccac caaaagtatt ttcattgtac 118980
atcatcactg ttttaaaact acggatattt atgataaatc ggagagcctg aatggcgtgg 119040
gtataaaagt gttcaaatcg cgtgggagta atttgttcgc gagcaactac cgtttcatta 119100
tagtttttca tgataagctg tactccgggc atatctgaga gctgtaccgg atcatttccc 119160
agtaattttc ttgtgccgta tagtagttta aactcggggg agccgctttc aaggttcggg 119220
taaagaagag gatcatatac ctcattattt tctattctta ggtcatgtaa ataatagagc 119280
gaaagtgaaa atggcataag aggctcctta ttgtaccggg acatatagtt ttgaatgaag 119340
tgttcttctg tttcaagata gatgggatga tcggtaagct cgtgcaggac ctccatggca 119400
gaatctgcca gagtgtgaga gcctctaatg atcccgtcga tcactgcgac cagtcgcttt 119460
cgcacaacat cgctcgtatt attttgtgcg tctcctaggg gcataagcgt aacattggga 119520
cgaaatacgc cgccaattcc ccgcagggcc gcctgaccga cggatagtcc tgtcgcagga 119580
acattgttat tattataata aataacggaa tcattattgg ctcccaagag tgccgtcaga 119640
ttagggcgag ctagttggac atttgtgtat tgtataaatt gttttagaag ctctccctgg 119700
ctaataagaa tattaaacat tttgttaaat agtggaagat tggctctata attttcttta 119760
aggtaaatgg gaatttctgt taaagtagaa ataagatgct gactcaggcc ctggcgattg 119820
gtatccttaa taagccgctg aagtataagt cccaaagaca gaagaagcac cgactgctct 119880
gtggggtcgc ctctatgacc aaagacgttg ttattgcgtg ctaagtcagg gtgagcatat 119940
cccatctcca tcactgcttg gctaaagttc ccattagcga atgcattaat aagatttaga 120000
tatatttttc cgctgggagc atcataaaat cgggtaatat atgaagctat gagctggtta 120060
aacaccatca tcatactacg attattttga ataccatagt ctgatccgta taggcgataa 120120
cgtcgaaggt tgtttgcggc atcattgaca ttggcatagg ttctgagcgc tatgttgtcc 120180
cagtagctaa gagtattttc ctcctgggcg ttgttggtac gaataagatt ggagagtcta 120240
aagtctccta gtgccacctg ctctacacga agtccagagt tattctccaa agcatcgtaa 120300
aatacgagtc tactgaatac tcttccgtat tgttcaaagc gttcagagga ttggggattg 120360
ttatttattt gaatattagc cgcgtccctt ctttgcgccc cacctcgaag ttgcagtaca 120420
ttataaggct ttgtaagcaa ggtgtaggtt ttattaatga tttggttaac cccctccagg 120480
cccaattcac cgccaggaag cggccttcct ccggcatcgg taggtggttt aataagtttg 120540
tcaattaaat gttcttccaa ccagtaaaat gagccaggat tagatctatt ttcatagtat 120600
tgaataatgt ttttatcaat atgcgggcgt agaagatcaa gaaaatactt cgtgtcggcc 120660
atcaaagaat caattaagga aataagacct gtaaaatcta aatgcacttg agcggtgctg 120720
gtttcaggga agcgaacttg aaccattttg ttaaaactgg aggtcatttc gaagatattg 120780
gtcaacagga gctgcatgat tcgctgatta tctactaaat accttgcggc caactcttgc 120840
tccggacgaa ctcctccacc agcaggaata cccacatatg gtacaatcca agcaaaaaga 120900
gtttctgtgg ttaaatttcg gtcttgggct gctgcagccg cttcggtagt gggatcaggg 120960
tacaccatag aaagccgcat attgatttct ttaatgacta atcctggatt tctaatctca 121020
gagatggccc cgtgttttct tccgagccag tcaataagat tggcgcggtt cacgttggca 121080
gcttgtgtct ctcgtaacca ttcgataatg cttttttgaa tcgtatctag gtctaaacct 121140
ttaatgttat tacgaaagtt attaagaagt acgtaaatag cactcaataa gttaagacct 121200
gtaataacgg tttcatgaaa cagaaatatt ttgttaacat ctgtatctgc cagtgactca 121260
gagccttgaa taagttttga aacgatttga attttatcgg tatgctcctt tttgagttca 121320
ttgatagcct ggcgaatgag ttcttggtag gaaattttgc ccaattcttg ttgcagactg 121380
ggatcttcaa acatctcact aagctgtttc ctaaattttt gtaccaaatc ccactgggag 121440
ttgggctgca gcattcctgt ttggacatcc acagagtcta tattgtatag tgccgggcgc 121500
cacttggggg taggctgggt tgaaggacta ataaacctat cggagggaag taattgtgag 121560
gattgtgtat agccatcctc atcaggaaga atggagtagt tggtttgatt catcattcca 121620
aaatcattca tagttcgcgc ttcctgaaca atgcgttgaa atttttccca ttcggtgcgt 121680
gtaatgacac cgaatctgcg gtttatttca tttacaaaat ggataagcgc ttttttggtt 121740
gcttcttgtt caccatactc taagttaaag tgttggtaaa tgacgtttat ttctttgata 121800
agctgacgaa tttcggtttc tgagtagtca ccaatgttaa taagctcaat aggacgcata 121860
aagataatgc gaataagtcc tgagaagatt ccttccagct caggaagcat cgagatctgt 121920
acattttcat ctctaaagga aaacaacttt tgataaaatt cggcgaggcg gggaaggcgg 121980
aagtaaagct ctgctgcctc gggaattacc tcgggctcta gctcatcggc accccccaat 122040
atcatacgcg tgggtataag tttgtacacg ggctcaggcc gttcaaacat gtcgtaaatc 122100
cctaatacaa taaaaatctt ggcggccata cttttcagca tgaaggtgaa gaagacgtcc 122160
tcggtttccc agcgggttga tagggcgtcg ttaactctca cagtagagag gtagacccgc 122220
tgagccgctt cctcggcagt ctgtgcaagc gccatccttt gtcctccaat ttctgattga 122280
tttagatttt taagtcccac ggaaagcgca gaatgttgaa gatattcaag caaggtttta 122340
tagatttgca ggggcgacat gggcaccatt tgccgcagct cctctccccc aagcatgtcc 122400
ccaatccggg caaaggcatt gatgatattt ttaagcgcct gaaagttaga aagagagcgc 122460
ccgataaggt cgcgaatgtt tttagcctgg cttgctctga cgggacggag ggtaccaacg 122520
cttcggcctt gttggatttc agccgcaact ttttcgtagt agtggcccgc aggagcatta 122580
tccgtaaaga cgttggagtc gttgcctgtg gaggtgggaa aactttcaaa gacttgtgca 122640
agcgtgtccc ctgttgtctc ggtgaaccat cgtcctataa tgcgcacgcc atccagcatc 122700
tgttggactg tttgaataga atctatgttg tttacaaacg ttttggtaat gtttttaaga 122760
taaagatcta gcccttccag agctcgatag aatcggcgtt ttacatcata ctccagctcg 122820
atggcgctta cggttgcctt ccagtctact tcctgggcac ctccaggatt tgggcccacg 122880
tgtcctctgg caagatctac agccggagaa ttaatgcgcg catttttttc cgtatccaac 122940
tgcatgaggc gtcccgcaat agcatctccg agaatagtgg catagttttc ctcgtaggat 123000
tgaaactcct gtttgttatg cgttaaattg gagtaaatct gggccacata atagtaatac 123060
ataaaggtgt taattgcctg gttgaggtca acctgcgatc gcgcggcctt gctgagccca 123120
agctcttcaa ctgttagggc agcaccgcct acccttgtac actcgcagtc ctcctcgcct 123180
ccatactttt tttgcacaat atcggtataa aaatcaataa tctgtagcaa gcgagagcag 123240
gagtcataaa gatttttaaa attagggtcg gttttagata tctcctccaa aacattttta 123300
acaagcgtaa gctgtgttaa gaaggtttcg cgttcttctc gtgcggccgc attggtgtaa 123360
aagccgataa gacttagatc aagtgcgatg gtgcccatat cattaatgcg cgaaagagca 123420
tctcgaagcc tcgttatgtt cggcgtcaag gcaatttctt taacaagttt gatgcctatt 123480
tttttcacat tttccaaaaa gtcgttatag gcttgtgtgc ttttattcaa aaattccatg 123540
aggatgtgct ttctatccag tctttgcgct tcaatcctcc tatctagtgg cgttttctcc 123600
tcatcgcccc cctttttggc acaactgttc tcaaggattt tgtggcgttc attaaaggtc 123660
tgtcgcaaca ggttcacggc tttttcaaac tcagcaatgt tttctgcgga gacaagacca 123720
ctaaaccttt tgaggtcaag ctccttgtca aactccgccc agtttttgct ttgaaggtac 123780
tgttcaacct tgagtcctac tttctggaga gccttattaa ttttattcgc aacagacgca 123840
gcaataccta gattacaaag tgtgtacgaa agtacttttc caaaattttt ggttcccaag 123900
acactatttg tatcatttaa aagtttaata atatccacct catccgtctg cagtttatca 123960
agttcctttt gggtgggagt taaaatattg tcaataaaat tcgttaaaat gttgatttgc 124020
aggttttgtt catttaaaag tcgacgatat actgcttcaa tcatggtgac tgcattaatg 124080
acttcctcat tgggggctgc tttggttacc tccgtcacca tgcgctcgtg aagttgctta 124140
atggcgtcgt ttaacagctt gatattttca agtgtatttt ctatactgcc gtgtacatca 124200
agatactctg cgcgcagtcc atgagttagg gagttaatgt acagaactat ttgtcgacat 124260
atactggcgg ccccttcggt ggtatctata agcttatcct gacctaaatc aataaattcc 124320
tggttaatgg cgtctgcaat cattttacag acggtctcct gtttttccgc attttttaca 124380
aaggtggaac cggctcgagg atcgggcagt tgttttttga tatctttaag aatatcttcg 124440
atgggctgct ttgtgtctac tttgaaccct attttggcaa tcgccctgat aattccttct 124500
ataatccgca gctttgcttt actcgatacg gagtctatgt gataatcttt aatgtgttgt 124560
acaggatttt tgtccccccc gccattaaaa tatcctcccc ctgaaaaagg acgagtttgt 124620
ctttgtatat gatcctgtaa cttcgcatat atatttgctt ctgatgaagg cagtggtcta 124680
ctagaggttg aagatccacg gttacccatt ataataaaaa aaaataaaga tttaaaacta 124740
caaatatttt gctgtttata aacccaatca tataagacta actaaaacat taaatgtagg 124800
tgagataaaa gcttattttt tttttaaaag tttaataacc atgagtctta ccacctcttt 124860
ttcttcttcc tttagagggg ttccataaat ggtttgaata aaattatgtg ctctaataac 124920
cttgttaaaa tcaggtgcct ttccatattg ttcaatatgt tgcacagtct tttgtgcaag 124980
catatacagc ttggagtctt taggtacctc cgatgagggc tcttgctcaa acaacgtttc 125040
aaaggaggat gtgcattcat tggtttcatt atcatttttt tcatgaatgt tctccgaaga 125100
tgctgaggat tccgtctcct cttcaaacag cacatgcaga atcatattcc attcttcttg 125160
agcctgatgt tcagtatacc cttgccctgc atatatacga gcagatttca caatatcata 125220
cttaacagta ctaagcaatg tttttatagc ggtcgtaaca attctaccgc tattgataat 125280
ctcaacagaa aaccaattat acaggctacc cgcatgaaac acaacttgtg aagatgatct 125340
taaatccgtt ttgaagatga cctccatttt catggatata tttaaaataa aatccattca 125400
attttaaaat tataaaataa taagaagatg ccctctaata tgaaacagtt ttgcaagatt 125460
tctgtatggc tacagcagca cgatccagat ttattagaaa ttatcaacaa cttatgtatg 125520
cttggcaatt tatccgcggc aaagtacaaa cacggagtta ccttcattta ccccaaacag 125580
gcaaagatcc gcgatgaaat aaaaaaacat gcctactcca atgacccttc acaagccata 125640
aagaccttag aatcactcat ccttccattt tacattccca ctccagcgga gttcaccggg 125700
gaaatcggct cctacaccgg agtgaaatta gaggttgaaa aaacggaggc gaataaagtt 125760
attttaaaaa atggagaagc ggtcctagta ccggcggccg attttaagcc ctttcctgat 125820
cgccgactag cggtctggat catggagtca ggctctatgc ccctggaggg tcccccctat 125880
aagcggaaaa aggagggtgg ggggaatgac ccgccggttc ctaagcatat ctcgccgtat 125940
actccgcgca cgcgtattgc cattgaggtg gaaaaggcct ttgatgactg tatgcgtcaa 126000
aactggtgta gtgtcaataa tccctatctt gccaagtcgg tctccttgct gtctttcttg 126060
tcgctcaacc atcccaccga gtttattaag gtactgccgc ttatagactt tgaccccttg 126120
gtgacctttt atctacttct tgagccctat aaaacgcatg gggatgactt tttaattccg 126180
gaaaccattt tattcggccc taccggatgg aatggtacag atctgtatca aagtgccatg 126240
ctggagttta aaaagttttt tacccagatt actcgccaaa cctttatgga catagccgat 126300
tcggctacta aggaggtaga tgttcccata tgttactcgg atcccgaaac cgtacattcc 126360
tatgccaatc acgtgcgtac tgaaattttg catcacaatg ccgtcaataa ggttacaaca 126420
cctaacctcg tcgtgcaggc ctataatgag ctcgagcaaa ccaataccat acgacattac 126480
ggccctattt tcccggaaag taccatcaac gcactgcgtt tttggaaaaa gctgtggcag 126540
gatgaacagc gatttgttat ccacggcctg caccgcacgt tgatggatca acccacctat 126600
gaaacctctg agtttgcaga gatcgttaga aatttacggt tttcgcgtcc cggcaataac 126660
tatataaacg agcttaatat tacaagtccc gctatgtacg gcgacaagca taccaccgga 126720
gatattgcgc ccaatgatag atttgccatg ttggtggcct ttatcaacag tactgacttt 126780
ttatacaccg cgattcccga ggaaaaggta ggggggaatg aaacccaaac cagtagcctt 126840
acagacctag ttccaacacg gctacactct tttttaaatc ataatctaag caaacttaaa 126900
atcttaaacc gcgcgcagca aacggttaga aatattcttt caaatgattg tcttaatcaa 126960
ctgaaacatt atgttaaaca cacgggaaaa aatgaaatac taaagttact tcaagaataa 127020
gtatgttgat acctgtggtg tgttttacct gtgggtttcc tattggaacc tacgcggcaa 127080
tttttgacaa ggctcgtacc gagtatatta aaaccaaaat gggcggaaca ttgccgcaaa 127140
atatcccatt agatgcttct ctccagattg agttaaaaga cctcattaca gctctgggaa 127200
tcccaatgcg ggtgtgttgt cgcactcatt taattactac gttggattat cgtaaatatt 127260
attaatatct aaaattgaaa aaatattttt aatgttacta gtaaaaatga ctacacacat 127320
ctttcacgca gatgatctcc tacaagcatt gcaacaagca aaagcagaaa aaaatttttc 127380
atctgtattt tctttagatt gggataaatt acgcacagcg aagcgtaata caacggttaa 127440
atatgttacg gtcaatgtca tagtaaaagg caaaaaagct ccgctaatgt ttaactttca 127500
aaatgaaaaa catgtaggaa ccattcctcc cagtaccgat gaagaggtta tacggatgaa 127560
tgctgaaaat ccaaagtttt tggtgaaaaa acgtgacagg gatccctgtt tgcagttcaa 127620
caaatacaaa atctcgccgc cattggaaga tgatggtctc actgttaaaa agaatgagca 127680
gggtgaagaa atataccccg gcgacgaaga aaaatctaag ttgtttcaaa ttattgaact 127740
gttagaagaa gcctttgaag acgctgtgca aaaaggtcct gaagccatga aaacgaaaca 127800
tgttataaaa ttaattcaaa gaaaaatttc taatagcgcg gttaaaaacg cagacaaacc 127860
tttgccgaat cctatcgcac gcattcgtat taaaatcaat cccgctacaa gtatactaac 127920
accaatattg cttgataaaa ataagcccat tactttacag aatggtaaaa caagctttga 127980
agagttaaaa gatgaagacg gcgttaaggc caatccggat aatattcata agcttataga 128040
atcgcattct atacatgatg gcatcattaa tgctagatct atttgcatca gcaatatggg 128100
catttcattt ccgctttgct tggaaatggg agttgtaaaa gtttttgaaa aaaataatgg 128160
gattgatgtg aactccattt atggctcaga cgatatttca actcttgtta atcagattgc 128220
tattgcttaa acaatttgct caaaacaagc ttataaacgt ttcttaggta tgcgatacgt 128280
aaatcctaat tctttaataa gttctttttc agtagtgatt tttagaggta ctaaagtttg 128340
atttttaaat aatccatact gatttagctt ataattcttt ttttttaacg cagctcgaat 128400
tcttattaaa taagaaacgg gacccgtaaa atgaagtact gcgtatggct tttcctcggc 128460
taaggccgta aaaagatcaa gttgatatgt gttttttttc cattcaataa aaagtacaca 128520
ctttcgttct ccgcagactt ttacagaaaa agaaagatcc tttatgcgaa tgttgggcag 128580
gacgtgtttt aaaagttttt tttctggaac aataataaga agatccacgt cattaagcat 128640
tttctcttcg cgtcttaagc taccaacagc aacgatgttt tttgataaaa tttttataag 128700
ttgtccatta tattcaaacg caagtcggga gcgtaagtca tttacaattt tttttccttg 128760
aataagcgtt aacattttat atttaatatt aaaatctttt cattttatat attatatacg 128820
caaaatggca cttgatggtt caagtggtgg aggctctaat gtagaaacat tacttatagt 128880
agcaatcatt gtggttatta tggcaatcat gctttactat ttttggtgga tgccccgcca 128940
gcaaaaaaaa tgtagcaagg ctgaagaatg cacatgtaat aacggaagct gttccctaaa 129000
aacaagttaa aacatgcaat tatatgcatg catataaacg catgcatata aacgcataca 129060
tataaaatgc gtaaatacta tataaaaaac tataacatat caatcaagga atcaacactt 129120
ttataatttt ccgtaatata tttttcatcc ataatgatgt cagagtacat ggtccctatg 129180
cgaggaacag agcccataag ggtaggcgcg gcaataccgt aaatgggatt cacggcggag 129240
tcaaccgcag catctgtcaa gacctggact ggagacgaca aggccattcg caacaacacg 129300
ttggaaggct ctcttgcatt aagccctgcc ttttctagag aggtaacctg tcccgttctt 129360
gtcatgagat ctgcgtacat gagtaaatga cgatggttgg gacccttgtc ccccataacc 129420
gttctaattt cactaataat tttttgccgt gccgcttcta tgccgtaaag ctccatggtg 129480
tctcctatag aggacgatac gatggtgtat gggtcgatgt tatcatcaag cattgcgcca 129540
aaaatattag tcccgtttgt tttgatggcg tagatattgt ctagtcttac cagtttcccc 129600
tgggcatcca cacggtggcg cataagctta acaacattcg catttttgat gcctggtatt 129660
cctctaatcg tgctatttaa tagtttatcc accacattta cggcaatttt ttcatccgta 129720
gccattcggg tattggtact gcgtctaaag gcgctttccc gtaggtatat gcgaataatg 129780
atgggaatcc ctgaggccgt gttttccaca gaatgcatga tgtaggtgtt ggggtgttta 129840
gctcttagac tattaataat actttctaga ctaatgcttt ttaatatcat ggttgttttg 129900
tttaattcca agcggataca ccagtttgca atatcctctg ggggctgtag tagaggatgg 129960
ttttccagaa aatccgtcat ccattccaca tcacttgcaa aatcggggta catcacattt 130020
ttttttgtgc ttgaatacgt ttcgtacaat aggtgccact gcaatatcaa ccgttcgaac 130080
gttataagct ctatgctgtt agcaatttct tgcgcatatg ttttatttgt ttccacttcc 130140
gggttcttta gacgtaaaag catttcagag gattgttcag cctctacggg cttcgcgcta 130200
aagatctcct ggggccgcac aattcccgac ttgttggttc ccccggccac ggaccggtgg 130260
tgggagtcca gcatatattg tgtcaagggc tctgatacgg actgcgccgc caggattccc 130320
actgcctcac cgtagttaat aagactttga gtatattgta gccttatgag gtccaggatg 130380
gcactcatct gctcgcaggt aatgtttaat gttttaacgg ttgccagttc gatgcgaata 130440
agcatgcgca tcagagaggc agcccgttta agataaacgg gtatgggcgt ttgtagtcgt 130500
tcctgaatgt tgttaataaa cacgtatgga agatttttgc aaaacgtttt gaccatcgcg 130560
tatttttgta gaatactttt ttcgtcgaag ggaagcacgc cactggtgga gctcagtaga 130620
atgtttttta cgatgctggc cacgtttacc ggcacctgtc taacatctgt aagcagctga 130680
ctgaaattaa aattttcgac gtttaggaag atctgtcgat atttatctct atccttttta 130740
aggcgtgaaa attcttcttc aaacaagggc gattgtatcc cggtgtactt gaatttgtct 130800
tcaagttcct ggtccgacag catgatggtt tcaaaccgta cggtttcaag ctggcgcgca 130860
tcaaggccgt cctctccgta caactgctgc acaagacgcg tatcgatgga aacccgtcgg 130920
taataatcca caatacagga ttgaaggcca aagatggctt tacggttggc atagcctgtg 130980
gatgatgtcg ataatgcttt gttgatcaag tcgaatcttc cattcatttc cccaaagata 131040
aattcagggg aggtaaggcc cgcaatatag ctgttgcaga tgaacccgta ggcctgcgcc 131100
tccagggcaa acctggggta gtacaccagg gtcctaccga aggaaaactg gggttgaatg 131160
cgttgtgtat taatttcaat ttggccgatg cccgccatga tgtgaatcat attggggttt 131220
gagcccttgg cgccagtggc caccatctga aaaagcccat tggtttccgg attaatggaa 131280
ttcataatcg gctttaaaat tctatcggga aatttaagcg cattcagctg caatttttcg 131340
tagaagtcat gcgttgtcag gcctataggc ggcatgatgt ctccatgaag cagccggttg 131400
tttatttcct ccgactcaag cagcagttca ttgataattt cttggacctc ctgatgtgcc 131460
tccggggtta ggagcatgtc ggccgtggac actgtgaatc cggcgttgcg cacgtagttt 131520
agggcgagct gctgggtcgc aaatatcatt ttcaaggcct gctgcggccc atacctacgc 131580
gaaataaggt gatagattcc accggaggaa cccgctccga cggccttttt gtcaaggacg 131640
ccttcaatga gttcgccgtt gcgtatttgt gtagagatgt cctgcttgtt ataatgcatg 131700
tagggtgcat acacttctga gtaccatgtg ggggctcgtt gataattgat gggggtctgc 131760
ctcagtagca tagatacaac cgatttgcca tccagcaggt cagttgggga gtagttggca 131820
aaacaaggtg ggtcggtttg ggttgtttga aacaacccca tggcgtgcag cttgttcatc 131880
acatttttcc ccatgggggt gttcgtgcgt gtaagcaaaa agcttcccac cgtggagtcc 131940
tgcacctgcc cattaacggg acccgagctc tttgtggaaa tgaaccagtt tcgcacagaa 132000
caaagtagtt cggcctcaac gcggctcatg acgctccagg gaacccagag attcatctga 132060
tccccgtcaa agtccgcatt ataccaggca catgcgctga cattcatttg aaacgtagaa 132120
atttttgggt tttcaagaac gacaatccgg tgaaccccta tgctgcttcg ttcgagagaa 132180
ggctggcgat taaaaaacgc gacgtcgcca gtgacgacgt cacggtaaag gatgtctcct 132240
acctccagcc taaagtcttg tttgagaccc tcaatgtcgt gaacggattg tgttatttgc 132300
ttatacactc ttgaacaacc agggtactgg cgctttccat ttaaaaaata gggcattaat 132360
ctattaatat tataatgttg cactgtttcc gcaacttgca gcgttcgtgc aaaggaaatg 132420
ggatagccaa cctcgtccag gtgaaggtct gagttcccgc agatggtgga ccggctgatc 132480
gaccatacct ggctgcccag tagggattta cgaattcttc cctccttgcg aggaagtctt 132540
cgcatgatgg agggagcagg gcgtgccccc atgacgatcc cacgctttcc cgtgcctccc 132600
tgggttgcgg tggtggaaac ggaatccaac aaaaagttat agtaaagttg ctgtatggtt 132660
tgcaaattgc ggtcaatatt taaaggtatt ttttggccgc gcacgatttg taggtccttc 132720
gggatcagca gattctttcg aaccagatac tgaatcacgt tgttaatgtc gtgaaagctt 132780
tgggggcctg acccgattcc caatctgatg ccaggtcgta tgctgatggg ggggatctga 132840
atggccttaa gcacaagttt ttcgggatgg gagtttttac ttcgccccag ttttacaacg 132900
gtgtcgtagg ttacgcgcga aaaaatctct ctgatgatct gcgggtacag tttgtcaatc 132960
ttgccctgct gatccgccca aaaggtaaaa taatcttccg agtccttaac aattttgggg 133020
tgtactgcct tacagacgta gcactgcttt ccttcggttt ggcttgaagc cgcttcaata 133080
agacgcttag gcctaataag gtgctcgtac ctctttaggt caacgatggg agccccgcag 133140
ttgagacata taacccttaa ccatcgtcgt atttcggcga tgaagagcgg ctgaagcacc 133200
ggagcatgca tctgcagtat cccagggtgt cccatacatt gcttgcgctg gtgtgagcaa 133260
gtgatgcatt tataatggtg atcggtggtt cccattcgcg catcatagat acccccttcg 133320
gcgggaaggg tgccctcaaa taaattagaa atggtaacct ccataacgcc ttgcctctta 133380
tgatcattgt caccggcaat attgaactga acggcggcta tttcggcata tccagcctcc 133440
atatttttgc taaatacata ataaaacttc aaatgttaaa aaaaaataac atcggttggc 133500
atattttttt gttaaaacca agtgttaaat gatttctaaa acacttatcg gttcacgaaa 133560
acctaccgca cgggcctgaa gaggaatgcc agttttgggg gaaagctcgg catattccac 133620
ggtaagctct tttccataaa gatgtttttt aaataaggcg ggcgtgagtt tttgaaaaag 133680
agcataacga tccgcgtacg tcaaatgctt aggagtgact acaaaccgct ttttgtttgg 133740
caattcgcaa acccataaaa tggcgcctaa gtcctttccc ttttttccct gagtatagtc 133800
cactaaaata aattcagcgt ctagcagcgg tttcagcttg gcaagatgcg ctgagtggta 133860
gttgttgtat cccggctcat agggcccatt ggcattgcgt acgatggctc cctcgtagcc 133920
ctccttaata aactgcgcct taagcctaag ggcctcatcc acattcttca cgctaaaatt 133980
ttcaacttgg tggataaagg taagatcttc cttctgttta aaaatatttg ttaatagctg 134040
ttgtctcttg ttggaaggca tttgaagctg atcactccaa aaacagtcaa acacgtaaaa 134100
gtgcagctcg gaggaatctg tcttcgcatt cgcctgcccc gcgatccatt gcagaggttt 134160
gcggtgtaaa taaagctcac catccaaata tactctcacg tctataaata aataaagctg 134220
tttgagctct tttttaatat tgtcaagacc taaaaattcc tttttcgtgc gcgaatacaa 134280
gagaatgcta ccatcgccct gctggcaggc cacagctcga acgccattac gcttgcgctg 134340
cacgatggga tctgtttctt cttcaaaaaa tgtcttagga attatattaa aatattttac 134400
cagcataggg gggataattc ctctatttgt gtgggctccc cgcttttgtc tggcatggcg 134460
attatattta ctaagggcgt ccttgaatgc ctgatggact accgttgtgg catttttttt 134520
acccaagttt tttccctcgg taacacgtgt catttttgat atccgcaccg ccccttcttc 134580
cacaaaaaat tttgtgaaaa tttcagcaac ggcgtctttt acatctgtgg aaaacatctc 134640
atctgtgatg ggaatgatcg tgttgtgctg caccacttgc acacaaataa tccatgaggc 134700
cttttttccg cttttcgttt cagactcaat cggaggaaaa caaaaaatgt tgtttgaata 134760
ttgcccagga aattgattta gcatggtttt aacaataaaa taagcctatc aattttttta 134820
taatttgaat agttattcca aattcaatat ggcttcttta gataatttag tggcacgata 134880
tcagaggtgc tttaatgacc agtctcttaa aaatagtact attgaacttg aaatacgttt 134940
tcaacagata aattttttat tattcaaaac cgtatatgag gcacttgtgg cacaagagat 135000
ccctagcacc atctcccaca gcatccgctg catcaaaaaa gttcaccatg aaaaccactg 135060
ccgggaaaaa attttgccgt cggaaaatct ttacttcaaa aaacagcctc tcatgttttt 135120
taagttttca gagcctgcat ctctgggctg taaggtctcg ctggccatcg agcagcccat 135180
tcgtaaattt atcttggact cctccattct cgttcggctc aaaaatcgta cgacctttcg 135240
ggtatctgaa ctttggaaaa tagagcttac cattgtaaag cagctgatgg gaagcgaggt 135300
ctctgcaaaa cttgccgctt tcaaaacgct tctgtttgac accccagagc aacaaacgac 135360
aaaaaatatg atgacgttaa taaacccaga tgacgaatat ctttacgaaa tagaaataga 135420
gtatacagga aagcccgaat ccctaacggc ggcagatgtt ataaaaatta aaaacacggt 135480
gttgacactt atttctccaa accatttaat gctaacagcc taccaccagg ccattgaatt 135540
cattgcctcc catatactgt cctcagaaat ccttcttgct cgtattaaga gcgggaagtg 135600
ggggcttaaa cgcctcctcc cccaggtgaa atccatgacc aaagcggatt acatgaaatt 135660
ttatccgccc gttggctact atgtaacgga caaagcagat ggaattagag gcatcgccgt 135720
cattcaggac acgcaaattt atgtggttgc agaccagtta tacagcctag gtaccaccgg 135780
cattgaaccc cttaaaccaa ccattttgga cggtgaattt atgcctgaaa aaaaagaatt 135840
ttatgggttt gacgtcatca tgtatgaggg caatctattg acgcaacagg ggtttgaaac 135900
aagaattgag tctttaagca agggcattaa agtcttacaa gcgtttaaca taaaagcaga 135960
aatgaagccc tttatttcgc taacaagtgc agatcccaac gtgctcctca aaaactttga 136020
aagcattttt aagaaaaaaa ctcgcccata ttctattgat ggcatcattt tagtagaacc 136080
tggcaattct tatctaaata caaacacctt taagtggaag cccacctggg ataacacatt 136140
agactttttg gtgcgaaaat gtccggagag tttaaacgta ccagagtacg cgcccaaaaa 136200
agggttttcc ctgcatctac tatttgtagg catctccgga gagcttttta aaaaattagc 136260
gctaaattgg tgtccaggat atacgaaact attccccgtt acacagcgca accaaaacta 136320
ctttccagta cagttccagc catcggattt tccattggca tttctttatt accacccaga 136380
tacctcgtca ttttctaata tagatggaaa ggtccttgaa atgcgttgtc ttaagagaga 136440
aatcaatcac gtcagctggg aaattgtaaa aatccgggag gataggcagc aggatcttaa 136500
aaccggcggg tattttggca atgatttcaa aacagccgaa ctcacatggc ttaactatat 136560
ggatcccttt tcctttgagg agctggcaaa gggcccttct ggaatgtact tcgccggtgc 136620
caaaaccggc atataccgcg ctcaaacagc acttatttcc tttattaaac aagaaatcat 136680
ccaaaaaata agtcaccaat cctgggttat cgatcttgga ataggaaaag ggcaggacct 136740
aggacgttac ctggacgcag ggataaggca tcttgttggg atcgataagg atcaaaccgc 136800
gcttgcggag cttgtttatc gaaaattttc gcatgctacg acccgacagc acaagcacgc 136860
taccaacatt tacgtgttgc atcaagacct cgcagagcct gcgaaagaaa tcagcgaaaa 136920
ggtacaccaa atttacgggt ttcccaagga gggagcttct tccattgtta gcaacctgtt 136980
tattcactat cttatgaaaa acacgcagca ggtggaaaac ctggccgttc tgtgccataa 137040
gcttcttcag ccggggggaa tggtgtggtt taccaccatg ttgggagaac aggtcttaga 137100
attacttcat gaaaatagaa tagagctcaa tgaagtatgg gaggctcgtg aaaacgaagt 137160
ggtcaaattt gctattaaac gtctctttaa agaggatata ttacaggaaa ctgggcaaga 137220
aattggagtc ctgttaccct tcagcaatgg cgacttctac aatgaatatc ttgtgaacac 137280
agcgttttta attaaaatat ttaaacatca cggcttttcc ctagttcaaa agcagtcctt 137340
taaggactgg attccagaat ttcaaaactt tagtaaaagt ttgtataaaa ttcttacaga 137400
agccgataaa acttggacaa gcctttttgg gtttatttgt ctgcgcaaaa attaaatatt 137460
ttttcataag aagtactacc caggttttaa agaaatagct aaaaatatca tatggatact 137520
gccatgcagc ttaaaacgtc tattggttta attacatgtc gtatgaacac ccaaaataac 137580
caaatagaaa ctattctggt tcaaaaacgt tacagccttg ctttttcaga atttattcat 137640
tgtcattact ctataaatgc taatcaaggt catctgatta aaatgtttaa taacatgaca 137700
attaatgaac gactgcttgt caaaacactg gattttgacc gcatgtggta tcatatttgg 137760
attgaaactc cagtctacga actataccac aaaaaatacc aaaaatttag gaaaaattgg 137820
cttctcccgg ataatgggaa aaagcttatt tcattaatca accaagcaaa gggctcagga 137880
acacttctat gggaaatccc taagggtaag ccgaaggaag acgagtcgga ccttacctgt 137940
gccatacggg agtttgaaga agaaaccggg attacccgcg aatattacca gattctccca 138000
gagtttaaaa aatctatgtc atactttgac ggtaaaacag aatataagca tatctacttc 138060
cttgcaatgt tatgtaagtc gttggaggaa cccaatatga atctttcttt acaatacgaa 138120
aaccgaattg ccgaaatttc taaaatttct tggcaaaata tggaggctgt acgttttatt 138180
agcaaacgcc agtcattaaa cctggagcct atcatcgggc ctgcatttaa ttttattaaa 138240
aactatttac gatacaagca ctaggatgcc gcattaaaat gccacataag gtaatacact 138300
aggaatgtcg cacacgcaca agaatacaac gtcgccggag atttattatc tagtacacgt 138360
tttatgtatg tacaatccgc cttcatttaa tatattgagc ggatgtacta tgtatttatt 138420
ttaacaaaaa acattatttt tttttaatct tcatcatctg tttttataaa ctcagtaata 138480
tcaaaagtag cttgtggggt ttcagagggt tcaccttggt tatcctccgt gaggataaca 138540
tgttcttcag gttcgtcgtc actggagaac ccatcattta attcctcttc actcaacatc 138600
tgtaaaaaat cttccaagct ttcgctatcg ttaaaatcct catcatccat aagaataatg 138660
gtaccttcct catcgtttcc tccttgtttc gtgtctaaat aggcctgcat ggcatttgca 138720
aaagtatcaa aataggctga gtcagattgc tgttccaaaa tatggccttg cgtattaaat 138780
gtggttgcat cgttgttaaa tgcttgcaaa tacagtaagg gatttatatc cattattatt 138840
aagcaaaaaa aatttaaatt atttttcgac cgatgttagg taaaattaaa caattgctat 138900
aggtgttaag caatgtttat tgattttaag tactcaacaa ccatgatgta aatactatac 138960
agcacttttg gatttttaat caaatccaga ttaatactaa cttcttttgt gatacagttc 139020
gtaataatag tatcctgctc atcgttttgt aagatttctt ttaatatatt tttttttacc 139080
gggatactaa gcaattgatt attttctttt aaaaactcct tttgatattc aatcgtctta 139140
ttcattgaat atttgtatat aactataatt acaaatgttc aatgaattgt tattcatgtc 139200
gggagatggc tatttaaaaa tcatgtccta tttttctttg ctcaataagc atccaaatat 139260
tttcatggcg ttttattaat tgttcattat tgaacgtatc acaaagatca tttataaatt 139320
gcagatagtt tattatttct ttcaagagag taacaaacat tacttcagca gaacatataa 139380
taggtaattc agtggcgtta aaagaatttt gatcttgttg atacgccaat ggcgaggact 139440
taaggagatt tgggggtctt gcccaaaacc ctaggctgct gttcttgttt tttagggcgt 139500
cataaagaaa tgaaagcaca ttgcaaggct taagccgcga catctccttc cccttgggcc 139560
ctttccatat ttttagatct aagatctcat ccgagcttat agagtaggta tagtaaagtt 139620
tttcaaaaaa gcatatctgc ttgaagtctt ttttagaacg actttcaaga agcatttcta 139680
taatgttaac aagttttgtt aggtttaagg cctgttcctg tgtaagctcc tcttgcacgt 139740
gatagactga aaaagtgtgc ttaggaatga aaatactccc cgtggcactg gcctgttgtc 139800
tgccaggtat atagtacacg ctgctgttag caagctgtac cggcacaatt tgccccactt 139860
ctgcaacatt attttgcgat tcggacgagg gtatgacaat agttacgggt tcagtcaata 139920
ggctttcgcc gagaataata ttactgtcat ttttaataat tttaacggcc gctattaaat 139980
caaaggcatt taagtaagaa acaacagcag aaaatcttac atgcatatat cctcttccgc 140040
tattattcgt acgcataata aaacaagggg agcgttgtat aacgccagta atattaagaa 140100
taaaactgtt tttgaaacac ttacccacat aaatgttttc aagctccttc aaaagatgag 140160
cctccacatt tgtacaaaaa ttggtaggat catcaatatt caacgttgtc tcaaaaattt 140220
tttggtcgat catatctata atatattctg tctatttcaa tttaaataat atacgaataa 140280
ataacgagat tattttatta aataagcaat ggtgtataca ctttgtattt actttgagat 140340
atactttgtg tatcacaacg tgccctaaga tgtgtgcaca agtgacggca ttttgtcgtt 140400
aaaaaggtaa aaccagcgga ttccatcctg cattccattt ggttgattac gagcctccat 140460
ttctttttgc aaaaggttat tgcgaatgag taagcagagc ttgatggcac taatctttgt 140520
aaggtttaaa cttatgccca attggtcagc aattttttgt tgctcctccc gtccgcgtgt 140580
ttcgcatacg gctccccggt ttagcatgcg aatatcagta atctcattct tttttaaaac 140640
ctggataggt gggcggattt taaatttaag ggcctttccc ttgctttcca tatagcctat 140700
gacgatgtcg ttttcttttc gtttaacatt aatattaagc atataaagcg gaatttcatg 140760
ccaggtttta tcttctcgcg aggtaataag tcgcacggag tcctccgtgg catagcccac 140820
tagagtgttg tcatccccag gcacgtggct tataatttta aaaatgtccg gaaatggctg 140880
aatatctttt tttgaaaaag cgatgaaaaa ctttttataa acctcgacaa gggcccccat 140940
acctgcaaga ttatctataa taagtgcttc tagcatcgta tagtgaaatg aagcggggta 141000
gtggatgagt acctgctcca ttggctcatc ctgaaaatcc ttctgaaact tttcatacaa 141060
tacttgaaag ggttctttgg tctgcgagtg ttcgaggtat ttggtaatac ggatgctgtg 141120
catcgcggga ggctgaaaat cccgaatata tgtttcaata tctaataccg gttccttttt 141180
atggttaagc accgcagcga cgtacaaatg ctcaggcttt gccggcacat gcataatggt 141240
gcaaagacga ttctgtatcc ataattcctt gcactggttt tttgagtagc atagagaaat 141300
gagcgccagc gcgaagttgt cctctgagaa gagtttatta tcgatggtaa ttccctgtat 141360
gagcttggga gtggaaacag ccttccatag ctcggagtac gtccacacgg ggcgtgccat 141420
aaacaaagat ataataatat tagaaattgt ttttacctct tgctccccgt atccataggc 141480
ctcaaaggta ttgaggacgg tggctccgac gtttgccggc gtgatggatg gactaagggg 141540
cagactttcc aacataggct tatcaatctt aatctggttg gtgaacccat caatggcgtg 141600
ctttcgcagc gccttatccc cctcctgtat taaaatgtat tcttttaatt tttgtgcgta 141660
cttagcgagc tctggccctc catcgggtgt tgtcgatacg tacaaataaa ttgtcacgtt 141720
gcgctcactg ggggggagct ccatgtgtga attttttcgc accaccctcc caaatacctg 141780
aataagccgg ggaatatcaa ggggcaatga cataatcatc tcgtaccgca cggcctgaaa 141840
gttcaaaccc tccacaatca ccttggaccc gatgagaata cgcagctggt ggccttccag 141900
gttggacgag gcgttaaaaa gagccaggct tcgttcgcgt acagcgggct ctatttcgct 141960
gtgcagaatg gtgaaccgta ctggaataaa ctgatggtcg ctatgtgtgt gctcatcgcg 142020
aatcgcggcg cagatggagc agcgggtcgt tcccacaggg gacgaaactt catttaaaat 142080
gccattactt tgtaaaattt cttgcaagat aagaaccccc gacatgcgga cccgattgtg 142140
gtaaattaaa attttccccc ggccttgccg aataatggaa agaatgtctt tcatcatttg 142200
agtgtatttt ccgctataaa aggccaatcc cgagatgtgc gttggtggct gcagcgacaa 142260
aaagctgcca ctcacattaa agggggctct acgcgaaggc tcaataatct gtaccccgtt 142320
ttccagaagc cagtctgtgc ttgccataga aagggcggtg ggggtttccg tcgagttaaa 142380
caggccgtaa gccttgggtt ccgtttgttt tgaaaatttt gggttgggaa acaccatgtc 142440
ataaatgctg tacgcattac tcgagatttt agggtcaggg cccagctgtt taagcgtttc 142500
aagctgatac tcagacatgg ggcattcgat gaaatgtaag tacggcaatg tttcgtcttt 142560
ataggacaac atctttccgg caaatattct ttcggggtaa aaattggtgt tggtatccaa 142620
caaaaaagat acccttccgg tgctcagtct ttccacaaga gctagggcgt cctttttcca 142680
tttaacggaa tgcccactgc tgtcaaacag ttgctggcgc tggaggggct ggccgttggg 142740
cagctcatgc cgcggaacca aaaggtttaa caggtcgacg tattccatga cactcccggt 142800
tacgggcgtt gccgacatga agacggccct gggggcctgg tgaggtggaa aggcatccag 142860
gacatactgt aaagcgatgc cataattatt tcgttcctgg atattgtaca cgttgtgtat 142920
ttcatccgca atgagcagtc ctcccctaag ttgctccatg attttttgat tcacccggat 142980
gaggccgttt gtctcggcct cgctaatttt ttgcacgaac tgagatatat cgttctcatt 143040
caatgtatct tctgcttcgt cagaacgatg aaacagagaa agcacatcaa agtttttctc 143100
ttcaccctta ctcgtaatat tgaaaagctt ggatgcaaat tccttatagc cgtaaaactg 143160
aaaaaagcct ccgcggtttc tatcggttaa acggcgcttt aacgtactaa cgaacccatt 143220
tagatgccgt gattcgaccg acgtggtgct gccagactgc tttgcaatgt gaagaagccg 143280
gtgtagctca gcgacctcct tgtaagaaac aaatcccagc tcaggacgtc ttagcatttc 143340
tgtttgaatg atggcgcgtg taaagcctac cacaaaaatc cagggcgcat tttcaataaa 143400
attcatgtag tggttcataa attgacgcgc gatggcaatc gcggcaatgc tttttcccgt 143460
cccggtctgc cagtttaata aaagacgcga gtagggcgtg ttgggatttt gaaagttttg 143520
gacgaaaagc tgggcattat gcaattggag acccttgatg gaaggaaagg gcgacgcgta 143580
ggggtcacac ggaaaaaacg ctcgcccccc cttctcgcag ccaggcccac cgatctggac 143640
aaaatgagcc cgcagatcac gaatgagctc tttttggtcg acaggagggg aaatcaacga 143700
tttaaactcc tttcttcgcg ccaactgctg caaaaagtct gcggcatcca attcgggata 143760
cgccatatta tcataaaaaa aataaacctt tttatgaaaa cttttatgtg attctgtatt 143820
gcaattgttt tttatgaata ctgtaaataa gcgtatcaac ttgtttttct aacgaagagg 143880
cgttattctt tttttctgga tataaaataa taataagtat aataattaag actaaacagc 143940
aggcaatcac tatcaaactc atattatact tactttttta taaaaagtat tatatcttat 144000
gaatgcgcaa gttcagctaa ttgttcgtcg cttggaatgt gggactgcag ggaggtggag 144060
tttttccttt ttctaaagaa taccgggaaa tggtggtgag gctcaggttg ttgtacatag 144120
tagctaggag gaggtttagg tatgctcgac ttgcagtcaa tagtccggtt atagtaaacg 144180
atggcaacga tgataagaat aataatgagc aaaatcaaaa tgcccaggag aatcgcagtt 144240
gttccgggat atttggcgat tgtatgggct aaaaggcctt gggtgctttg tttaattccc 144300
tcgcgggttg acaggttatg agaaagcagt ggagacgttt cagtgtccat ttattacaat 144360
tgaacagtta tattaatctc aaataaaata taacacaaaa ttaattatgg ccatgcaaaa 144420
gttatttacg tatatttacg agtttattga atatcgtaag atggtgctgt tggaagaaaa 144480
ggtaccatat gataagtttg ttcaaatggt acttaataca ggattttttc gtattaacgc 144540
ggagacgctg aatcacggaa tcgtatccgt gtttatcttt ggagcaaatg gcaagtacgt 144600
tcaccacgga ggcgacatga gaacgctttt aacgaatacg cttaatgaaa aaaaacatta 144660
tgaagaatta attttaatcg ttgataagcc cgttttaagc aaaaaaaata ttttagatat 144720
aatcgtcgag cagcgcgctg caaatcccac gattgtaata aacatatatc cctaccacct 144780
gttctgcatt aacattccca aggtgagtgc cattcctaaa cataaactaa ttactcagga 144840
ggaggcgcag gagtttttag gtcgcgaata tctgcaaccg caggacctca tgcaaattag 144900
cgcgtcagac cccccggtgg tctggctggg aggaagaccg ggagactttg tgcaaattga 144960
gcggccctca gagacagcta tgcacgctgt tgttatccgc tttatcacca agtccaaaat 145020
ttgagtcccg tgtttaaaga tgacagacag ctaagtaagc atatctgtaa aattgtcgat 145080
gtcctctgtg gatagagcgc tttcctctga gcagcaaatt ttttcataca tctccatggg 145140
ggatggcgag gctttaatag tatgtaggtc acgtaagaac tgttgtatga tgggatattt 145200
gtcttttaaa aactggggat gtttcataac tggaattatt tgaaagataa agaccttcca 145260
tccaaagtag ccaaccacat ttggcatttc gggacacgcg gtttcataag gcatagaata 145320
gtgaatagtg tactgatctt tttgatacag cgtttcaagt agttggcgaa atgtttccgc 145380
gtcgagcgtg ccaaaatctt gaggagcctc ggtgtgctcc tgtgtagagc agatcgtgat 145440
gattccccag gcaagcggga gcatggactc tggagggtgg atatccgtat tggtctcatt 145500
attcgatccc agctgatgaa tgccgcacac gcgaaacatg gcctcgacgt agatgcccat 145560
agagataggc ggcgaaaggg caagaccgga ttgtatttgc ggcatatagt aggagggcac 145620
cgagtttttt atttttcggt tgaatgggga ctttatttct accagcacgg ggatgcgttt 145680
cgtggcctca tagcgtacgt tgttaaaaat tgttttgatt tcccaggact gttgagtgta 145740
tcccagcgtt aggtgacaaa acccatcggg gctattacta tgtccggggt atcccaaata 145800
ggtcccatca atatgaatat tgtcacctat gacggtggtt tggcagaaca actcaagcag 145860
atctttacta acacgctcaa aaagggttcc ccagctacaa gcagcgcggt tcaaattctt 145920
cttaaaaaga tttgcttttt ccgccaaggt tatataatag cttttgtaag ggtttaaacc 145980
taaaacgctg gcaaggtcag agccacccac ctgagtgcga cgaatagcat gccaggcatc 146040
ggagcgctgc tgaggagagt ctttaaacag gcgtacaaag gtttccatta tacttgtttt 146100
aacaggaatt caatataaaa agtcaacaca gtttgcaatt tttccaatct caagatatag 146160
ccatacattt ttttttccaa ttggcgaata tgtttaagct catgtgtttc aatattagca 146220
tccggaaatt taaatgcata aagatgttca aaggcctgat ttatacacgt atcaaaggat 146280
ctgtggtatg ttattagctt cagcatgtgt gccagatctt caagatggtc taaatttata 146340
cggttttcca cgtggtggat catgtctgcc acatcttgag cccccatcca ggggatcaca 146400
aggtactccc ccttaaagat gattcgtcgt ttttttaaaa aatcatgaaa acgttttaaa 146460
gcttcaagaa aggggcagtt gggctttgac cccaaaatgc tgacgacgat atcctcgggc 146520
atgatgtatt cgcagtgagg atagtagttt acggactcta attcagcggc ccgccgtttt 146580
atttcgtatc ttgcccagtt attcagagag tactccacgc ctccgaccac aacagacatc 146640
ctatctatta aaaaataaca ataaaaacct tatgaaatct atgtatagtg gccgctaaaa 146700
tgtctatatt agaaaaaatt acgtcaagtc cctctgaatg cgcagagcat cttacaaaca 146760
aagatagctg tttaagtaaa aaaatacaaa aagagctcac ctcttttttg gaaaaaaaag 146820
agacactcgg ttgcgattcg gagtcctgcg taattaccca ccccgccgtg aaggcctatg 146880
cgcaacaaaa gggactggac ctctccaaag aactggagac tcggtttaaa gcgccaggac 146940
ccagaaacaa cacgggtctt cttacaaact tcaatattga tgaaacgctg cagaggtggg 147000
ccataaaata caccaagttt ttcaactgtc ctttttccat gatggacttt gagagggtcc 147060
attataaatt taatcaagtg gatatggtaa aggtatataa gggagaagag ctacaatatg 147120
tagaaggcaa agtggtcaag cgtccttgta acaccttcgg atgcgtttta aacacggact 147180
tttcaacggg cactggaaaa cactgggtag ccatctttgt ggatatgcgg ggcgactgct 147240
ggagcatcga atattttaat tcgacgggaa attctcctcc aggtcccgtt attcgttgga 147300
tggaacgggt caaacagcag ctattaaaaa tacaccacac cgtgaaaacg cttgcagtta 147360
ccaacattcg tcaccaacgg tcgcagaccg agtgcggccc ctacagcctg ttttacatca 147420
gggcacgcct cgacaacgtg tcatacgccc attttatatc cgctaggatt accgacgaag 147480
acatgtataa gtttagaacc catctgtttc gcatcgcata aactaataaa gtttgaattc 147540
tttataggaa taaaaatgga agcgtttgaa atcagcgatt tcaaagagca tgcgaagaaa 147600
aaaagcatgt gggctggcgc cctcaacaaa gtcactattt cgggtcttat gggggtcttt 147660
accgaagatg aggaccttat ggcgttaccc attcacagag accactgccc cgctttgtta 147720
aaaatttttg acgagatcat cgtaaatgcc acggatcatg aaagagcttg ccataacaaa 147780
acaaaaaagg taacttacat taaaatttcg tttgataaag gtgtgttttc ttgcgaaaac 147840
gatggcccgg gaatccccat tgcaaagcat gagcaagcca gtcttatcgc caagcgcgat 147900
gtgtatgttc ccgaggtggc ttcatgtcac tttttagccg gaacgaacat caataaggcc 147960
aaggactgta tcaagggggg aaccaacggc gtcgggctga agctcgccat ggtgcattcg 148020
cagtgggcca ttcttaccac cgccgacggc gcgcaaaagt atgttcaaca tatcaaccaa 148080
cgcctagata tcattgagcc tcctaccatt acaccctcca gggaaatgtt tacacgtatc 148140
gagctcatgc ccgtatacca ggaactaggg tacgcggagc ctctgtctga aacagagcag 148200
gcggatcttt ccgcctggat ttaccttcgc gcctgccaat gcgcggccta cgtgggaaaa 148260
ggcaccacca tttattacaa tgataagcct tgccgcacgg gctctgtgat ggcgctagcc 148320
aaaatgtaca ccctgttgag cgcgcctaat agcacgatac atacggcgac cattaaggcc 148380
gacgcaaagc cctatagcct gcaccccctg caggttgcgg cggtcgtgtc ccccaagttt 148440
aaaaaatttg aacacgtgtc cgttatcaac ggggtaaatt gcgtaaaagg agaacatgtc 148500
acctttttga aaaagactat taatgaaatg gtcgttaaaa aatttcaaca aacgattaaa 148560
gataaaaacc gcaaaacaac attacgagac agctgttcaa acatctttat cgttatagtg 148620
ggttccattc caggaataga atggaccggc cagcggaagg atgaacttag catcgcggaa 148680
aatgttttta aaacgcatta ctccattcct tctagttttt taacaagtat gacaaagtct 148740
atcgtggata ttcttctgca atccatttct aaaaaagata accataaaca ggtcgacgta 148800
gacaaatata cgcgtgcccg caatgcggga ggaaaaaggg cgcaggactg catgctactc 148860
gcggcggaag gggatagcgc actttccctg ctgcgcacgg gactaaccct gggaaagtcc 148920
aacccaagcg ggccctcctt tgacttctgc ggcatgatct ccctgggagg agtcatcatg 148980
aatgcctgca aaaaggtgac aaacattaca acggactctg gagaaaccat tatggtgcgc 149040
aacgaacagc ttaccaataa taaagtgttg cagggaatcg tgcaggtatt gggtctagac 149100
ttcaactgcc attacaaaac acaggaagag cgagcaaagc tgagatacgg ctgcattgtt 149160
gcgtgcgttg atcaagatct ggatgggtgt ggaaaaatcc ttggactgct gctggcctac 149220
tttcacctgt tttggcctca gcttattatc catggtttcg taaaacgact gcttaccccg 149280
ctgatacgtg tgtatgaaaa gggtaagacc atgcccgtgg aattttacta tgaacaagag 149340
tttgatgcct gggcaaaaaa gcagaccagc ttagccaacc ataccgtaaa atattacaag 149400
ggattggcgg cgcatgacac ccatgaagta aaaagcatgt tcaaacattt tgacaacatg 149460
gtgtacacgt ttaccctgga tgactcagca aaggagttgt ttcatattta ttttggcggg 149520
gagtcggagt tgcgaaaaag agagctttgc accggcgtgg tgccgctcac cgaaacccag 149580
acgcagtcca ttcatagtgt ccgacgaatt ccttgcagcc tgcatctgca agtagatacc 149640
aaggcttaca agctggatgc catcgagcgg cagattccca acttcttaga cgggatgacg 149700
cgggcgcggc gcaaaatttt agccgggggg gtgaaatgct tcgcctccaa caaccgtgaa 149760
cgaaaggttt ttcagttcgg gggctacgtt gcagatcaca tgttttatca ccatggcgac 149820
atgtcgttaa acacaagtat tataaaagcc gcccagtatt acccaggctc ctcccacctc 149880
tatccggtat tcataggcat aggaagtttt ggctccaggc acctgggagg aaaggatgca 149940
ggatccccaa gatacatcag tgtgcagctt gcgtctgaat ttattaaaac aatgttcccc 150000
gcggaggact catggcttct cccctacgtc tttgaggacg gccagcgggc ggaaccagag 150060
tactacgtgc ctgtgttgcc gcttgctatt atggagtacg gcgccaaccc atcggagggc 150120
tggaagtaca ccacttgggc ccggcaactg gaagacattt tggccttggt gagggcctac 150180
gtcgacaaag acaacccaaa acacgagcta ctgcactatg caataaaaca taagattact 150240
atactcccgc tgcggccctc caattacaat ttcaagggcc atttgaagcg gtttggccaa 150300
tactactaca gctacggcac gtacgtcatc tcagagcagc gaaatataat tactattacg 150360
gagcttcctc tgcgtgttcc tacggttgca tacatcgaaa gtataaaaaa atcgagtaac 150420
cgcatgacat ttattgaaga aatcatcgac tacagtagtt cagaaactat tgaaattctg 150480
gtgaaattaa agccaaatag tcttaaccgt atcgtggaag aatttaagga gactgaagag 150540
caagattcca tagaaaattt tctgcgcctg cgcaattgtt tacattcaca tctaaacttt 150600
gtaaaaccta aaggtggcat tatcgagttt aacacgtatt atgaaatttt gtatgcgtgg 150660
ctaccttaca ggcgtgagct ttaccaaaag cgtcttatgc gtgagcacgc ggtgcttaag 150720
ctgcgcatta tcatggaaac tgctattgta cgctacatca atgagtctgc agagctaaat 150780
ctttcccatt atgaggatga aaaggaggca agccgcattc taagcgagca tggatttccc 150840
ccgctgaacc acacgctgat catttcccct gagtttgcct ctatagagga actcaatcaa 150900
aaagcactgc agggctgtta tacctatata ctatctttgc aggctcgaga attgcttatc 150960
gcagccaaaa ctcgtcgggt ggaaaaaata aaaaaaatgc aagctcgtct tgataaggtt 151020
gagcagcttt tgcaagagtc tccctttccc ggcgccagcg tatggctgga ggaaattgat 151080
gcggtggaaa aggctattat aaaaggaaga aatactcagt ggaaatttca ttaaacgcta 151140
ccggttttat gatgtccaat aggtgttaag caatcagttc atcaacattt ttttcaagaa 151200
tttgaaaagt ttggataatg ttctgaatac ttttttctaa aagagttatc aaatcttctt 151260
gtgaggcctt atgaataatt gttaatacca tttcttgctt atggggaaca cactgatacc 151320
ccacaaagct aatatcagga atcatttcat aaatatatgt ttttagcaga tttccgatgg 151380
tatgggtttc atcttttatc gtgataatgg cctttgtttt ttcctcatcc atggaaaaca 151440
gcacaagttc cggctgcggc tcttcaaagt tttcataaat tttttgaatg ctttggattc 151500
ggccaataat gatccggcag gcgtttttta aatacgtgcg aacggcctgg ttgatatgtg 151560
gcagcggcac cgctggaaag caaagcccca ggcggtggtg acgcgggtct gaggtcatag 151620
agctttgctt gtaaccgcta agcgccatat attctttttt atccgttggg tactgttcaa 151680
tgtcaaggtg ggaaaaatgt gttttaacgg caagattaaa ggcggcatgc tttcgtccta 151740
tgcccttttt aatatagata tcctctataa tcaacgattt tccgggttgt aggaagccaa 151800
tctcaaaggt aggattaaaa atcgggtatt taagcttagg gcctgccacc tggatgagat 151860
cgcggctata gatggtttta acctcacagc tattgtttaa actccgcaga gcaaatacca 151920
gtgtctcgtt tttcgcataa atcggaatga aattaatgcg gtttctaata aattgttccg 151980
tcataaacag gtccgtggaa tcctcgatct tatacccacc gggcttaata tctagcatat 152040
aattgggaat ttcatcttgc aagacccgcg acaggccgtg gaccgcggct ctgctaatgc 152100
ccttaaagtc cataacaaca ttgaccggga cgaggggcaa ctgctcctcg agctgaaata 152160
gttttttggc cgcattttta ataaagaggt tggaaaagtc tatcaaaaac ggtttgattt 152220
ccacgttttg gaaaattttt tccatttgta ttataaatat atctatatat attcaaatta 152280
tggtagttta tgacttgctc gtttctttaa gtaaggaatc catagatgtg ctacggtttg 152340
tagaggcaaa ccttgcggcg tttaaccagc agtatatttt tttcaatatc caaagaaaaa 152400
actcgatcac gacacccctt ctcattacgc cgcagcagga aaaaatttcg caaattgttg 152460
agtttttaat ggatgaatat aataagaaca atagaaggcc ctccgggccg ccgcgtgagc 152520
agcccatgca cccattattg ccgtatcaac aatcctcgga cgaacagccc atgatgccgt 152580
atcaacagcc cccggggaat gatgatcagc catatgagca aatataccat aaaaaacacg 152640
cgtcgcagca agtaaatact gaactgaacg attattatca acatattctt gcattaggcg 152700
atgaagacaa aggtatggac agcatgttaa aacttccaga aaaggcaaaa agggatagcg 152760
atgatgagga cgacatgttt tctataaaaa actaacgacg taacaattaa acaaaaaata 152820
aaaatcatta taaaatgaat cttgaatacg tccaagttgt tcaaaaattt aatcaagtac 152880
tcctagaact taccaaaaaa gtatgtaccg ttgtgggcgg gagcaaaccc acctattggt 152940
atcaccacat tagaagggtt tgctcagaat gtccatccat gccgatgagt atgataggtc 153000
cgtatctgaa tgtctataaa gcccaaattc taacaaggga caagaatttt tttatgaatt 153060
tcgatcccgc gcataatgag tacaccttta tcattcaaaa actaaaagaa gcagcccgaa 153120
atatgccgga agacgaatta gaacagtact gggtaaaact tttattttta cttaaaagct 153180
acataaaatg taagcccttt attaattaaa gaattgatgc ataactaata aatggccggt 153240
cgtgttaaaa taaaacagaa agagctcata gactctactg taaaaaacaa aaatgtgatg 153300
aatctgttcc atgaaattat aggctcaaaa ggcaatatta attttagcgt tgtctggccc 153360
aagtttaaaa aaatcaaaca gagcgtttat gactacattt ccactctttc tgtgctggaa 153420
aaagcaaacg ttatgcaaaa ctttgaagct gataagaaac tgttggaact ttttgtacaa 153480
aagctgtggg ctgcctatga aggctatttc aaatatcccg agattgaaaa atatgaggtg 153540
gaaggccagg taaatttcaa tctcgtacct cagtgcgtcc tcgaaaagtt tagccagttg 153600
tataggataa gaatcaattc agagcttgtc acactcatcc taaacagctg tgcctttatg 153660
agtaaatata acgattatat tctcaaaaaa gatccctaca tactaaccat aacccccggc 153720
ctatgctttt cccccattcc caacttcgag gacctaaatt ttaaacatct ttacaacagt 153780
gataaaaatt ctcagcatga caaagagttt atcatgttta tattatataa gctttatacg 153840
gctgccctag gagtgtacaa tgccatctcg attccagaca tcgacgtaga agaccttgaa 153900
aatatcatcc tatcctcggt gagccagatt aaaaaacaaa ttccgcgctg caaagacgcc 153960
ttcaacaaaa ttgaatcttc ggtacacctg ttgcgcaaaa attttaacac atattacagt 154020
gactatgtgg gctcaggcta caacccaacc atcattatgg aacagtacat taaagacata 154080
tcacaggatt ccaagaacat atcaccacgc atttcctacc agtttagaac catcatcaag 154140
tattaccgcg acatgattgc caccaggcat caaacgatgg acccccaggt attaaacctc 154200
gtaaagcacg tcgaaaagaa attagatatg cttgatagag aaaaaaatta gtatatatag 154260
ttatggtgaa tctttttcct gtttttacct taattgtgat tattacaatt ttaattacga 154320
ctcgagaact atccaccacg atgcttattg tttctcttgt aacagattat attattatta 154380
atacacagta tacggaacag cagcatgaaa acaatacatt tttcatgccg caaaaaaatt 154440
cttttaacga atcttataat aaagacaaaa aatctaatat acatattccc taccagtggc 154500
tggcgcctga actgaaggaa gctgagagca agtactggtg gggcaattat gatcctcata 154560
gcgagcccgt tctcgctggc gcatcttgaa tatcttcata cgtggcacgt caccatcaaa 154620
aacattgccc aacagcacgg gcttgatata aaggtggcca ttgtggtctc aacatcgcat 154680
ttaaataatt ttttgccaat ttccggggcg cttaacatcg aatgtataac cttccccagt 154740
tgcggcatca aggagataga cctcctatgg gcgcgcatta aactatttca acattactgc 154800
gccatcggtg cccgtctttt atggctggta agtgctgaca tcaggccccc tgtttcagcg 154860
tggccagcca tcgccgacag tctaaaaaag ggagcagatg cggtcgttat tccctacccc 154920
tcccgatgga acaatcttat acctaccgtc atcaaagaaa tagttgtcca ccaaaaaaaa 154980
tgccttgtgg cggtggatgc acgccacctt gatacagata cccagattgt aggggccggg 155040
atgggctgca tcgtcctaac cctaaaggcc cttatggtgc gcctaagtat tggcaaacag 155100
cccgttaaga tactgtggcc cgaccttcac ggcactgccg agggcattcc tctggagggg 155160
gtggaggttg gctggttttt aaacgcttat gcgcataaat taaatatacg ctgcctaggg 155220
gctgatcata ttgcgcagca cttaacttaa ttctttattt aaaaagtcca cgcatccagt 155280
ggcggcctac attaagggcc tacgcacata aatatacact ggctagaagt acgccttcat 155340
ttaaaccatt gaattattta tataatggct gcaaacatta ttgcaacaag agccgtgcca 155400
aagatggcca gcaaaaaaga gcatcaatac tgtctgctag actcccagga aaagcgtcat 155460
gggcattatc ccttttcatt tgaattaaag ccttatgggc aaacaggcgc aaatatcata 155520
ggagtacagg gctcacttac ccatgttatc aaaatgacag tatttccatt tatgattcct 155580
tttcctttac aaaaaactca tatagatgat tttattggtg gacgcattta tttatttttt 155640
aaggaactgg acatgcaagc agtttctgat gtaaatggaa tgcaatacca cttcgagttc 155700
aaggttgttc ctgtaagccc caaccaagta gagcttcttc ctgtgaataa taaatataaa 155760
tttacatatg ctataccggt agtgcaatac cttaccccaa tcttttatga tctttcggga 155820
ccgctagatt tcccattaga tactctttcg gtccatgtgg atatcctctc caatcatata 155880
cagcttccta tccaaaacca taacctaaca acgggtgatc gtgtttttat ttctggatat 155940
aaacacctgc aaacgattga attatgtaaa aataacaaga tttttatcaa aaatataccg 156000
ccgctttcat ccgaaaaaat aaaactatat atactaaaaa atcgaatcag aattccgcta 156060
tactttaaat ctttaaaaac gtctaagtaa taacattttt atagtctact cctagttccg 156120
aaataggctg aatttctttt ttaagtcctt taaaccaagg atgtgataca agactcttaa 156180
aggaaagccg cttattttca ttaattgtta aacattccgt gataaactgt tttcccgtct 156240
ctgaaatgtt ctcgggaata taattttccc gtttcaggat atcatttaaa taaaaatttt 156300
ctgcacgaaa tctaaaaaga ttaaccgcga ccatacctat cgtccacacg gttaaaggaa 156360
gctggtagta ataaccataa taataaaatt ctggacacac gtattcccat gttccaaaca 156420
tattatattg gggacgggtt tcgtctaatc taacagcgct tccaaagtca atgaccttaa 156480
tgatcttttg atttatgtct ataataaggt tctcatcctt aatatcccca tggataaagc 156540
ccttctcata aatgttttgt ataataagaa taagctggaa tattattttt ttggcttcgg 156600
tttcctcaag ttttttaaag taatgataat gaagtagatc aacactattt ggaatatatt 156660
ctatgattag tatatgatac atagcatttt cggtatattc gataagctta ataacaccgg 156720
gagtatcttg cagggctttc aacacgatga cttcatttcc tggaatttct tttttagaaa 156780
cgtacttaaa tataatgggt tgccctactt gatgacccaa aaagacgtta tttctgccac 156840
cctcaaacat gggtctcgtc gcaatgaaat acatgtgctg cgttgtggag atcctttcca 156900
cctttgctgt aggataaaac gcatattgtg cctggggatt ttttaacatt tttttaagct 156960
gttgttccgg cctggacatg ttttattagc tttatatata aagggttaga aggtttaatt 157020
tcaatatatg ccttaatgat gggattatat tcgtaaaagg tatagcctaa tcctacgtct 157080
ttgttttttt ggtaaaaaaa ctgtttgccc tcgtaggata tgctataggc ttttacttcg 157140
gcttttacaa gcggttggca gggattgggc aaacgtaaat cgcgttcaaa gttttcatga 157200
aaaagcaaag catttgtggg ctgacacatc agacagccgc tttcgccatt gaaggcacat 157260
tcaatggccg ccctttttag taaatcgcgg aaagcagaat taagatggct cttttcaagc 157320
cccctttcgt gaaaacgctc atcaatcgtt ttttgttcct gactgccttc gggaatacta 157380
taaaacattt tttgattagc caccgcgatg tacaaaaaag gctgtacggt tttctcctcg 157440
ggcggtagcg catcgtggct accaatgcgt ataatgcgcg ccttcacttg atcctctcgg 157500
gccttatccc agtacggctc taggatatga acctgccgcc cgtatttgag atccaatccc 157560
tcagctcctg ttttagagac gagtaaaatt ttaataacct ctccgtgtat attcagcggc 157620
gaattccaaa gctgctggat catgtcgcgc tctttagata aaattttccc tgtaataagc 157680
gtaaatcgtg ttattttgga ggacaggact aacgtatggg tcggcccatc ttccgcaaag 157740
tttttcacca taagatcttt cccatcctta tgaaggagga tggtgttgtg cccttcttcc 157800
aatactttta ggggctgaag gcactggtag ccctctattt ctaaaaagcg ggccacgacg 157860
tgaaggccca attccacaaa ctgtgagtaa atgagcacag ggcccggaga cgttttaata 157920
ttttttagca tgcgtactat tttgggacta gaattttctg tgaaggcctc tttgggcagc 157980
tgctgaacag cctctgataa tttttcatcc tcctttactg ttagcatttc ggacgcgaag 158040
atgctgatca tacgggaacg cacatagtag gaggagcctg actcttgctc cgatcctggc 158100
aggcagaggg cggcggcatt tattttttca tacattcctg agctggcgtg cttttccgcg 158160
ttttcaacgt ctcgggccag cagatattgc ctatactgct cgggtgacat ttcaaccttt 158220
tctataataa gaggaagctc tgtggggaat agcttgttga gctcattctg gtttccagcg 158280
tagcttatca tacccactag gcggtttagt agtttgtccg cgtttaaagg gctattcgtt 158340
gttttattga cataagcggt gtagaatctt tcatagtgaa gaggtaataa gattcgcccg 158400
cttagcatat taaaacaggg caccatttca aaggggtcct tcgaacacgg ggtgcctgtt 158460
aaaaacagaa tacgaatatt tttagcttgc ataatattat tgtacagctg gcgggcattt 158520
gttttatcat tggcgctatt gataattcct ctaaagaggt tgtgtgcctc gtcaacgatg 158580
agcaggcatc catttaggga ccctcccgcc tttatgatct gctgccccat gttgtaagcg 158640
tctagggaca caaacctgaa gcgccgcgag attttttgta gctctttgga gtgatccgtc 158700
gtttccggat ataaaagttt aataagcttt aacaaagact gttggaagtt tgagtgcaac 158760
gacttgggtg cgatcagaat cgggttgtaa atatgtgaaa gtgagatggc aagcgacagg 158820
ctcaaaatgg ttttccccat gcccatctgg tgatagatga ggaggccccg tgtgttttcc 158880
ccctggccta tcccaaattt aggatccgaa aaggcggtgt aaattaaaaa ctggtagtat 158940
ttcagggctc gtgcaaagcg ggcagtgagt gaggtgtctt tgctttcctg aagctcttta 159000
tatttttcat atacctcttt taggtatgct tctatttgga cggggaagga ggtgttgttg 159060
tgcacgcaag acatgactcg ttataaggat cccatattaa aacttcatta gaagaatagg 159120
gctgctgata gctagcgctg cacttaaaaa tggggtagcc ctttttcttg taaatccggt 159180
gcctgtcgta gacctggcta gaaagcgggc ttagtgtatc tttaatgtcc acaacgatgc 159240
gtaccttttt ttcatccgat ccctgccggg taatacgtcc caagatttgc tccatgttgt 159300
ttctgcgggg cgttgccatg atgatcgatg tcatatgctt gaaggaaatg cctctacgcc 159360
cgtagccata ggtcagcaag ataatggaag cgctgtgtgc ctgagaaaga gcggtatttg 159420
aaaccccgcc gcataggagc gccacctccg gaacgataat ttgaacatct ttgaattctt 159480
tggaaagcgc ctgataaaaa atttctaaaa gtttgcgaaa ttccacgaaa atgatgatgc 159540
catacggctc atcggtcccc catttgtgag gctcagcggt atgcagggag taaagccgct 159600
ttgcctcatt tacgacaagt tgtatacgcg aaggatcttg aagtagttta tcaatggtgg 159660
caatggccga taccttttca ttaatataca cagggctaac gaagtcagga tgtccctgat 159720
attcgatttc cctcacgtac ccggaaaagg ttgtggtggg acttacagtc ctctggggct 159780
gtcctagatg gtgaataata atcttgtcca taccatcggg ccggtccagg ggtgtagcgg 159840
acagtcctaa tatccgacta agttgtattt tccaaaaaat tttgtaattc tccggcgagt 159900
gtaattcatg tgcctcatct aacacgacta gaccaaaggg ctcaaagaac tgctcaggct 159960
tcttgcgcag ggtattaatg attcccacga tgacgtcgta ctctttgctc gtcatgtcct 160020
ttttcttgca cgctgcatta ttgtaagcag ctacacgtag gtggggcagg agcaatgtta 160080
gctcgtcgat ccactgtatt tgaatcgcct tggtgggcac gatgaccagg gtagggtaca 160140
aaagtttttg aataatgctg atcgcaatac gcgttttccc caaaccggta tttagatgta 160200
ggtaaaagcg cccatagggg gacaggagct ttttatgaat cttatcgacc atttcttgct 160260
ggtagttaaa tagtggaaat tctgtttcaa cgcatgggag ggcccgcagc gacacggggc 160320
gcgtcgtgta aaccatgtta aacatttcaa actgcttttg cagcaatatg ggaaaataaa 160380
tgtattcccc ctgcagcgtg aaggcagttt cctgtcttat ggctatgtgc tttggctgcc 160440
cgggtaatgc ccgcgccgta acggtgagcg ccttaagaac gcgcccgaaa tcatgttgta 160500
atttactttg tagcttctta taatttattc ctattccagc aaaggatata atggcctcca 160560
ttctcacgct ggacgggtta tatgcagagg ttccaaaatt cttaccagag gcgttacgag 160620
agggctgtgc tggcaagaat cctctaagct tttatattca acaaatttta aatttaatgg 160680
gatgtgacgg taacgagtac catgttcttt ttaccagcag ctccgaggaa gcaaatactc 160740
atatgatcat ggccgccgtg cgtcgccatt tgctgcggac gcagcaaagg cctcatgtca 160800
ttatcggagc agccgagccc cctagcgtca ccgaatgtgt gaaggcattg gcgcaggaaa 160860
aacgctgcgt atacaccatc atccccctaa aaaattttga aatagatcct gttgcggtat 160920
acgatgccat acaaagcaat acctgcttag cgtgcatttc aggcactaat gctgttgtca 160980
aaacgttcaa caaactccag gacatcagca acgtgttaaa aggtattccc ctgcactcag 161040
aagtgagtga tcttgtttat caaggatgta ttcaacaaaa tccgcccgct gatagttttt 161100
caataaatag tctctacggc ttcctgggag tcggtgtttt gggaatgaag aaaaaggtca 161160
tgcaaggatt ggggccgctc atttttggag gagggctgag aggcggaagc cctaatatac 161220
ccggaattca tgccatgtat aaaacgctaa cccagcaaag gccttctatg aaaaaaataa 161280
atacaataca tacgctgttc atgaaaactt taaaaaaaca tcagcatgta tatctaccca 161340
tagggggcgt gtctgcagag gacacgtctg cagaaaacat atctacaaaa gacatgcctg 161400
ttgaaggccc gaagggactc ccgggctata ttttatttag cgttggccgt cgcgccgagg 161460
agctacaaaa aaaaattttc actaaattta atataaaggt tggccgtgtt gttgacttac 161520
aagagatact gtttcgtatc aaaatacccc aaaaatactg ggagacatta ttgttcatcc 161580
aattaagaga taatttgacc aaagaggaca taaaaagagt tatggttgtt ttgatgcatt 161640
tagataccat cactcctcgt ggctctcttc ctcctccgag ccactcttct tctttttctt 161700
aatcgttttt gtttgttcta taataaggga aaagaactcc gtgggatctt gttccccgta 161760
caggttatct gcgaccataa ggatgcttag aatggtaaac aggtgagaat acataagggt 161820
ttgcgtttta agaaaaccct gacgttgaat cataattgaa aacaccttgc aaagccgact 161880
catcagttgt tctgtaatgg cgttaagcat tttctggaat ttttcttggt tttcgggtgt 161940
gattttatat tcatgtagaa agtgtttcac acctgaggag aagaatcttt cctccttcga 162000
gagcccatct ttgatgatgg gaagttcctt gatcagggca aaccattcct cctcttgggc 162060
ttgcggattc tgaagatact gatggcagat atggtttaga atggtgcaca cgtagctaat 162120
aagctctgag ctgattcttt ggttggtttt caaatgttgg cgaaagtagt ttttcaccga 162180
agtgcatgta ataaacgtct tcattttctt ataatataca acagtatgtt gagtctttaa 162240
tttaaaatta caaggagttt tctaggtctt tatgcgtata ggtgtttctt tgtcgtaaat 162300
tttcaatagc cgacattgtt tgtgaagcag tgttctgagt agtgactgtc gtgtaaggct 162360
cagccggatg agcaggagca ctcgcggccg caggtgcggc cgccggcccg ccagttgcca 162420
tgactagtct gtccgtaact gggttgtccg taactggttt gtttgttgct ggtctgtttg 162480
ttgccggtct gcccgtgact ggcttgccta cacttgctgt agtcgctcca gctggtttag 162540
aggtacctgg ttgtggagtg acttctaccc actgctgatc ttgataagga tttataaact 162600
gtatatcttc ctcctcaata gcagcagctt ttttctttct tgaagagaat agatagatta 162660
gaacgatgat aatgatgact aagaccacga tagcaatgag aatagtatac atatgtgtgg 162720
agaagaagct tggtgtagtg actggtgaca aacactcacc ataatgccgc ggataaaccg 162780
gttgaaaaaa ttcagaatcc atttaagata ctattataaa taatatataa aaatgttgtg 162840
gcgcaatgaa attacagaat ttatggacca actttccaag tattctcaag aaatcttaaa 162900
aacgtttaag caattgcgtc ctagtgaata taaacaatac aatgaatttt taacacaagt 162960
tacaccgttg ctgcaaaaaa cccctgaaaa aattccagag ttggttgacc atatattcaa 163020
ttacctagac aacgttgaaa aaatttgtga gctcctcgtg aatgctagct caattattat 163080
tagttcaaaa atacgagaac aagtaaaaca cggaatgagc ttcagctata aagccgacct 163140
cgactccttg gcggacattc tctctcaaaa acagtacgtg cttatgcatc tttcaaaaaa 163200
tattgcggcc gagtatttta atacgtgttt aaaccaaggg aaatccaagt tagatctcaa 163260
agctgcctct gtattttata gtagtcgttc ccgaacggca agctcagcag aactctatag 163320
aaaaatgcta tacgcctatg gttcaccgca ggaaattaat tattatactg aaaaagcccg 163380
aaataagacg ttggatgtgg aggagagcga cagcatggcc atcatcgaac gaacggcccg 163440
acacaacctt tcccttatgc acccgctaga agccatgggg cttacctttg gggcaaccaa 163500
cacggacgcc gacccggagg atctgaagga caaaacggtg ataaatttaa cgctcccgca 163560
ggcaacagaa agcatcacct accatcttaa atccctaatg cagctaaaaa aagtaagtac 163620
ggcttcagga ctaaatacaa acattttgaa agcatttgat aatattattt ccacccctgt 163680
gaaaaaaaat aaaatggcct ccaagttggc gcccgggatg gatgtcgtgt tcactagcga 163740
taacggaaaa acatttttta ctaaaaacat tttaagcaaa aacatgctag cggggcccaa 163800
agagcgggtg tttgcatata ataatctcat tagtaattta aataactcct gtttcataca 163860
aaatcacaac gattttttaa gacagcagga ctcttggccc ttctatgacg cgcacaattt 163920
taccaacaag tttttaatgc agcctatttt ttcggggcag acccgtcctc ggcttcaggg 163980
agccatggag gcggcgcatg tggaaacgca tctcacggca tttttacaaa gtattcagcc 164040
ctctaggcca caagatccct ctgttttggc ttcccccaag ttatctgctc taatcttgaa 164100
ctaaaaacag cctttcttgg acttaaatga tggtctacca gtttttgaaa taacttagag 164160
aactatgaag attttcatga aatttaaatt agagatttgc aaaggttact tgcggtcatt 164220
ttctgttgaa ttaaataatt attcgaatag tataatgtct gaagatattc gtcgtggtcc 164280
tggcagaccg ccaaagaaaa gggttgttcc caactttgag cgcaagggca ttctggaaaa 164340
accagttcgg ccacaaagcc gtctcgagtt ttcctatgat aacccgctga tatttaaaaa 164400
tctttttatt tactttaaaa accttaaaag taaaaatatt ttggtgcgat gtacccccac 164460
cgagattacc tttttttcac gtgaccagtc gcaggcaagc tttgttattg ccaccatcga 164520
cggaaaaaac gtgaaccatt attacgccag tgatgtcttt tggctaggca tcaacagaga 164580
gctcgttgaa aaaatgttta acagcattga tcgctctttt ttaaaaatta ccatcgttca 164640
ccgctatgac aagcctgaaa ccctgttttt tatctttacg gattttgaca ttgacaagga 164700
gtgcacgtat cagattacgg tctcggagcc cgagctcgat atggacctta tcgaaatgga 164760
aaaaagcatc agtgaagaaa gactcaagaa ctatcctctg cgctgggagt ttacctccaa 164820
gcagctcaag aaaacattta gcgacttatc aaactacacc gagctcgtga ccattgaaaa 164880
actcggcggc gatacgccgc tgcacctgta tttccaaaag tttaactcca tctcatacca 164940
cgagatgtat aaatcttcca acaagatcaa cctgacctcg accattccta agtcgcaggt 165000
gttccagata aatgttaaaa ttgctcacat caagtcgctg gcctcggcta tggtcaccga 165060
caagatccgc attctgtgcg aagaaaatgg gaacctaatc tttcaatcgg aaatggatgc 165120
ccttatgtta aatacgatta ccttgaacac cacgatatag ttcggtaaca ttagatgttc 165180
taatatttag catctaaata atacgctgta gtccggtcag ggttgcgtca cagttttccc 165240
atttttttgc ctcgtcggcg gtggccaccg ttgccctatc atttacgccc ggtaagacaa 165300
agctaaaggc gttcagcggg gcttggcaat gcccgcccag cgtgaaggag ctcggaggat 165360
tttgcgcatc ccgaaatccc ttagccatgt tgtttaacac ttcggttacg tcaatcgagt 165420
gaagggatcc cttgggatcc gtgaatgtaa agacgcagtt tctaaagcgc atgtatgcga 165480
tggacgattc atcgggggtt ttgaaggtaa cagtgttccc cttgctgtac ttaaaggggg 165540
accatccggt aaaattatac caaatgaaag caataataat taaaataacc aacacaatag 165600
ttatagacaa cacaaagtct gtagtgccgc ccattattaa ataaaaatat tttagaccgc 165660
cggcttaaaa tttacttatt gctcatagct taagtctatt ttattcatag cttaagttta 165720
ttgctcatgg cttaagtcta ttgcttatag cttaagtcta ttttattcat agcttaagtc 165780
tattgttcat ggcttaagtt tgttgctcat agcttaactc cattactgat agcttactga 165840
tcatgactta aataaaaata ttttgcccgc ttaaaaattg tttaggtttg aaaaaataag 165900
agatggaggg ggcaacttat cgtcattgtg tttaccccca ctggaagaca tcaaacggta 165960
aataattata agaatcaaaa tgattaatat aagggttaaa aaaggatgat tcatcacatt 166020
aattaaaaac gtatttataa cgctgttgca gttgaaattt tggtataggt cggaaatatt 166080
gcccgagcct ccgtattctg caatgttctg acatatggtg agtccggagg ggcactgctt 166140
gttggtcaaa atatttcttt gctccgttgt tttataggca tttttatttc cattacacgg 166200
agcaaacgca cattcagccc atagggtgcc ggagttcaca caggcacaat actggctata 166260
cgcatactca tcctttgagc acaatccctg tttatcgcat atgctcccaa taatattgtc 166320
atcctccgcc gtttgttgat ttgtatgcga gcgtaaaata gcggcccagg ccttgggctc 166380
ctttttttgc agctcggaaa tcgaagggcc tgtacagcta aagtcgaccc aaatatcatt 166440
gcatttcgtg gaaactggca tgcaagacat aattgaaata attaataagt atatatcatg 166500
gcaacaaatt tttttattca acctatcacc gaagaagctg aagcatacta cccaccttcc 166560
gtgataacga ataaacggaa ggacctgggg gtagacgtat actgttgctc cgacctagtg 166620
cttcaacctg gactaaatat tgttcgcctg catattaaag tagcatgcga acacatgggc 166680
aaaaaatgcg gttttaaaat catggcgaga agcagtatgt gcacccatga acggctgctc 166740
atccttgcaa acggaattgg tttaatagac ccgggttatg tgggcgagct catgctcaag 166800
atcattaatc ttggcgacac cccggtccaa atatgggcca aagaatgttt ggtgcagttg 166860
gtggcccaag gtgaccatgt gcctgaccat atcaacatcc taaaaagaaa ccaaatattt 166920
ccgctgtttg cgcctacccc aagaggcgag ggtagatttg ggagcacggg cgaggccggg 166980
attatgagaa cttaatttta ttttttttct taacataatg ggaggctcta caagcaaaaa 167040
ttcctttaaa aatacgacca acattatcag caattccatt ttcaatcaga tgcaaagttg 167100
tatttccatg ttggatggca aaaattacat aggcgtattc ggtgatggaa atattttaaa 167160
ccacgttttc caggatttaa acttatcatt aaacacaagt tgcgtgcaaa agcacgtaaa 167220
cgaggaaaat ttcattacaa atctttcgaa ccaaattact caaaatttaa aagaccaaga 167280
agttgcgtta acccaatgga tggacgcagg aactcacgat cagaaaacgg atatagaaga 167340
aaatataaag gtaaacttaa caaccacact tattcaaaac tgcgtttcat ccctgtcggg 167400
tatgaacgtg ctggtggtga aggggaatgg caacattgtt gaaaacgcaa ctcagaagca 167460
gtcgcagcaa atcatctcta actgcttgca ggggagcaag caggccatag acaccacaac 167520
cggcatcact aacacggtaa atcagtactc acactacacc tcaaaaaact tttttgactt 167580
cattgcagac gcaatttcgg ctgtttttaa aaacatcatg gtcgcggctg tagttatcgt 167640
tctaatcatc gtagggttta tagccgtctt ttactttttg cattcacggc accgccatga 167700
ggaggaagaa gaagctgaac cactcataag caacaaggta ttaaaaaatg ctgccgtttc 167760
gtaataattt aattaaaagt aaaaaaaaaa ggtattgtta tagtgatggc agattttaat 167820
tctccaatcc agtatttgaa agaagattcg agggaccgga cctctatagg ttctctagaa 167880
tacgatgaaa atgccgacac gatgataccg agcttcgcag caggcttgga agagtttgaa 167940
cccattcccg actatgaccc taccacatca acttccctgt attcacaatt gacccacaac 168000
atggaaaaaa tcgcagagga agaggatagt aattttctac acgatactag ggagtttact 168060
tcactggtcc ccgatgaggc agacaataaa ccggaagatg acgaagaaag cggtgcaaaa 168120
cctaaaaaga aaaaacattt gtttccaaaa ttaagctcgc ataaatcgaa gtaaaaattg 168180
aagcgaaaaa aagtagaaaa aaaatgtttg gagcttttgt aagccaccgt ttgtggtcag 168240
atagtggttg tacgaccacc tgcatcacaa acagcattgc taattatgta gccttcggcg 168300
aacaaattgg atttcccttt aaatcagctc aggtatttat tgccggccct agaaaggctg 168360
tgataaatat tcaggaagat gataaagttg agcttttaaa gatgattgtt aagcacaatc 168420
tttgggttgt tgctcatgga acctacttag atgtgccctg gtcccgtaag agtgcgtttg 168480
ttacacattt tatacaacaa gaactactta tatgcaagga agtcggtatt aaagggttag 168540
ttttacacct aggcgctgtg gagcctgaac ttattatgga aggactaaaa aaaattaagc 168600
cggttgaggg ggttgtcatt tacctggaaa ccccgcataa caaacatcat acatataaat 168660
acagtacaat tgagcagatc aaagaattgt ttttacggat acgaaatacc aggttgaaac 168720
agattggttt atgcattgat acggctcaca tctggtcttc cggtgtcaac atctccagct 168780
ataatgacgc ggggcaatgg ctgcgctcgc tggaaaacat tcattccgtg atcccaccaa 168840
gccacattat gttccaccta aatgatgccg ccacagaatg cggaagcggt atagaccgac 168900
atgcaagtct ttttgaagga atgatttgga aatcatatag ccataaaata aagcaaagcg 168960
gtttatattg ttttgttgaa tacgttacgc gacaccagtg tccggctata ttggagagaa 169020
acctcgggtc ttccatgcaa ttacaaaccg ctttaaccgc agaatttact acattaaaat 169080
cgttattaaa ataaggatga gttttagcga atgtccctta gttattagtg catgcaaaaa 169140
atttctacaa aagcgtatta caatagagaa tgaagcactt ataaatgcct taataaccgc 169200
tttagcgcag accagcacgt tgaatgatct ttgtttatta cctattcaaa cctatttgct 169260
tagttataaa aatgcttttg agtggataca cttcgtatgt attgcaatca ccactatttt 169320
ggataataag tataactgga aggactgtac ggtagatatt aattatattt ttctccatgt 169380
aacctatatt tacaatatta aaaccaagga atacctagac tactgttctt aaactttatt 169440
ttttctatat ttacgccaaa gagaatattt aaagtttttt ttgaaaaaaa ataatatatg 169500
tagataaaat tcagttacat gatatatgtg taaacatgtg tggtaaacaa catatggtta 169560
tgctttataa gataaatgcg cataatatat gtaaacaaaa tatggttatg tgttaaatgc 169620
atataaatgt attttaacgt atatcttgtg ataatggata tatgcattta ttaaaagagg 169680
ctgtatttat tataaatctt gctaaggatg ccattgtcaa catatatccc atgttggaca 169740
aattgcgttg cgatccagtt cttttttttt tgattttgtt taatgctatc ctttttgaag 169800
ggatggttgt ccaccatatt tattcgatgt tcaatgaata ggtctgcttt ttcgtaaggc 169860
agtgaaggtc gttccaagac tccttgaacg aaggacgtgt tttcttggat ccacttaaaa 169920
agcacgtggc attcaaaaac aggacagtga ttggatcctt ggatatgctt tggacagcca 169980
atgcttgaag agatgtagtc ccttttcttt aggacaagct tctccacgct ggggcaacag 170040
agatcgttca agttctggac ggtcgcattt ggaatgttga aacttcgtat ccattcaccc 170100
tcgggtcctc ccttatgaag aaggagtatt tgctcatggt ccttagtaat cttaaccaaa 170160
tgttggaaga tcattttttt acctgcttta aaggcctgaa gggtgtcagt tggcaaagct 170220
attgaattcg ggagtgggct ttcatcaagc gtgaaatggt gaatgtgacg cgactggaaa 170280
gaaaacgacc gttgatttat tttttcaaag attgggtcga ttccgccatg aaagaacagc 170340
tgcaagattt tagaaggcgt atttttttcc caataaaaaa tgaccacttc tcgtgggatt 170400
aaaatcgtct gtgtcccatt ttcattatat aattggccca taaagccatc aacgtcaatc 170460
aacaccaaaa gcatggtata gagagctttt agaaccggag ttcgttaaaa aaatacaaag 170520
ttcgtttaaa acgtgtaatg ttactaaaaa aatgtaatgt ttaaatgata atgataccac 170580
atgcattaat gaaaaaaact tttaaatttt tgttttaata tttgcatgaa aatggaaaca 170640
tttttagtct gtttatttca caatgcagat ggtttacatc aacagattca ggaaattttg 170700
tatttattgc ggatgcatat ttacgaaaca aatctttact taaagcagga actatcacgg 170760
cttatatatc caaataggca actttctttt gtgttactta tgcccctttc ccttctaaga 170820
aactgggatg acattgaata tttaacggac gttgtagatg ataagcagac tctacattac 170880
gcggcaaatt tgctgacaaa ctacgttcta catctatcca tgtttcaaaa gctgacaaaa 170940
ccatacttcc ttttagcggt caagcgggtc agcgaaaaac tcaacaaaaa gcagcgacat 171000
tcattttacg aggtattggt aacctccgaa accttgaata attatgaaaa cctatctaaa 171060
aacattttaa atacgttgat gtttgccgtg cgctacgtat ttaaacctac gccgaactat 171120
tcagaaattc tcgcagagtt ggaaaaaaaa aataaaattc accatattat ttttaatatg 171180
gtaattacgg attttgcgca aatccgtgaa caacaaatgg ataaacatct gtgtgaaaca 171240
aataatgagc ttcgtcagga atgtaaagaa actatttttg atttaaaggt ggtaggaaat 171300
gtttagccaa taaactcatg cccgcatttt ttacaggtac aaaatatcgt ggatggctca 171360
tcgagggcgc gtgtttgtac ttctctgtag gtacacatac gctgcttgca gttgggacac 171420
ttataaagtt gtgacgtctt ttcggcgacc ttttgctgcg aacgtagagt aatttctgtc 171480
ttctccttta aggcggcaga ggggcaaagc tcggcgaacg tcatgctacc aattgcctcc 171540
ggttttagct cgccagaaat tagcttatta agggcatcgt tatcctgttg ttggtgactt 171600
tttttttcgc agttaataat atgattgatc gtcccacaac gggttgaata ttcttctaaa 171660
aaggtttttt cttgttgctg gtacgtataa tgataacacg aggcctcgat tttttgcgcg 171720
tattcggtgc ataaatcagt atgttcctta aaaaacatat gtttttgaag cgttctaaaa 171780
aacatcattt ggatgatatc acgcatttcc aaaataatat agggttctag tcttttggaa 171840
tctttcataa ctagatcggt ggtaatattc ttagtcatac aatttattaa aaatggttta 171900
atatattgta aatatttttt aggcgtgtca gcctgtaaaa aacattcttg ttcaatctta 171960
tttgtaagga tagtattttg caaatactta tttagcaaaa atacgataga atcgcgggct 172020
atatgcattt tcatataatt ttttttttaa aatttaatac aaaaaaaaga agtatagact 172080
cttcttctag tccggttagt tcgttggttg cctcaacatg gagactcaga agttgatttc 172140
catggttaag gaagccttag aaaaatatca ataccctctt actgctaaaa atattaaagt 172200
agtgatacaa aaagagcaca atgtcgtctt acctacagga tctataaata gcatactgta 172260
cagtaactca gaactttttg agaagattga taagacaaat accatttatc ccccgctttg 172320
gatacggaaa aactaattgt aaccagtagt acatttaagg atagtttaag cagtaaatgt 172380
agaataacac agttaagcaa taaataacaa gtatatagga atatatagga atatatagga 172440
atatatagaa atatatagaa atagctaagc ttaatactaa ttcagctttt tttttaacta 172500
aaacctgaat agatgcgaag tagcggacat atacatacta aaataagcca tacatttact 172560
ttcttcttga acatgaaacc tttttttctt ctgttgttgg tatataaaca ataggactgt 172620
ttgctgaggt tgtatgatct tctacaactg ctgtctcagg atgacgatgt ttttttaaac 172680
taaaagtgta ggatggaatg agtggaatat agttatggct cgacttatcc tgtttcgtac 172740
aggaatattt tttacaaata gaacgcaaca agcatatgaa taaaaacaga aatgatatac 172800
aggagcataa aatagatatg aacactaagg ggtagcagct tttataacgt tccgtatttt 172860
tcttagctat caattgattt accgtaatat ttatctcggg aaactttgtt ctacaatatt 172920
ttgtttggta ttccagaaac tcatgtcctg gcttattccc gcagcttaaa aaatgataca 172980
aaaatgtgtt attgttacta aaattaattc ttcttaagaa aaactgcgga agacgcttta 173040
ggtacgtctg ttcctgtttt agtaggaagt agtataaggg acaatttctt tttccacaca 173100
ttagattatt gtaatatagg taggttgggg tgttggagcg aataagtttt ctgagtatgt 173160
tataatctat gacttgtaaa tcgttatacc ttaggtccaa aaacttgagt tctttaccaa 173220
agccacctgc aatttcagaa atatttttca tcccgcagcg gataatacgg atgtcctgaa 173280
acgtctttaa aatacttgta ttgtagtgaa tacttatgtt atttttttgt aaataatcta 173340
tgtcatgaca agtgcatgaa atgccagcag cattgcttgg tatagtatta tatgcaggaa 173400
gaactatact actattgaga atagtcacat tgtacttata ccatgtatta ttttctgata 173460
taaagtattt gcaggtgacc tgtggtttaa tcctacctgt taagccactt cctaaaaaaa 173520
caaaaaatat gaaaaccctt agcatcctgt atatactatt aaaaatttat aaaattttct 173580
gtttaaattt catttagaca aaaaaaataa tatatataca tcagcaagaa attatataca 173640
gattatataa ttttctgatt tttttttgcc acaataagca tcattatatg cattaaaatc 173700
tcaatactaa acactaaaat ctaaattcta agcattaaat tctaagcatt aaattctatg 173760
cactaaactg taagcactaa aatctaagta actaaaatca acactaaatg tatgcaacct 173820
aaaatgtaaa gcattactca tcatcctcct cttcttcatc ctcatcatca taggttaaga 173880
tatatgtgtc atcctccatt tcttcacatt catcttcata agcatcactg ggtattggtg 173940
gaacattgga tgcagcattt ttaaaatatt ctatgtcttc tggtgaacac tcatctaatg 174000
attttttgac agtcctttta acttccatgg gatatgattc caaatcctct ttatataaga 174060
gtttacggta gcttttagct gcatccacat ttgctggaga atctggattt ggctcattga 174120
gcagtgaaat tacactaaga agaatggtat caatcttttg agccggagac caagtcattc 174180
cctgttcttc agcattgtct ccgtgtaaga tagagataca tagttttcca tcagagtaaa 174240
tattaggatg ccacatttca gaggtgaatg ttaatctggg tggtgcatat gggtattctg 174300
gaggaaaggc gatttttgcc ttgaataagc ctccctcata aaaagtgtca ggtgggcccc 174360
ttaagatcac atcccattca gtcatatcct tctcattcac cgaaattttg aaattctcag 174420
agggattctc tatcaggtgt ctgtactctg ctattaaaaa cctggaaacc atggttattt 174480
aatattaatt aaattccctg gtttattcct ccttaaaagt agatgaacct cttttgtttt 174540
ttattgggtt catttttact aaatttatga actggaaaaa actttaacgg cataattatc 174600
aaatgcgaag ggggatccgt ataaaatcct agcttgccgg taatggctat taagttaaat 174660
ttggtaccag taacactaat atttaaaaag ccctgatcat taactttcca cattaaaaga 174720
ttattatatt cgaatgtttg tccaatatgg acaactttgt caccagatgt tacatttgat 174780
ttggttgtta gtggctgaag cttggcacaa tcaaaaataa gcccattaac actaagatat 174840
agaggagtgg gttgatctat tttctcatag tttaatattc catctttcca cgtaatagct 174900
tgataattat ccgcagcaat gagttgaaat tttataaata gtacaggggt tttagttgtc 174960
gttatacatt taaagggtgt tttataaaaa taaaaaataa taattgttaa aagtatgata 175020
ataatcgcca aaataatttc atacattttt tataagaatt atacatagta tggtatttaa 175080
aatattagct aaatttaaaa aaacttcatg atttttaaaa cagggaaaaa ggggattagg 175140
ttgaataaaa aaggtaagca cttgtctata tatttttttt acaatgttgc cttgagtcgc 175200
atttttaact ggctggggag tatcagagtg gaatatcact gtagtaggtc tataaggtct 175260
tgttaaaata tgatcggtca ttgttttcgt actagtgtca tttagggtcg acctgatagc 175320
tcgatataaa gttatagggg ataacctatc aaatacagtc ttatctgtgc tgaaatgtat 175380
atcgtcttct ttatcactaa taatattagg aatggctgtc attaaataat tactacttgt 175440
tgttgtgggt gaaatagttg tactggtatt attggaaatg gctgtcatta aataattact 175500
acttgttgtt gtgggtgaaa tagttgtact agtattatta gaaatggctg tcgttaaata 175560
attactacct attacaagta aactaatgct aactacattt ttaacctcaa taaacctaaa 175620
aagccatact aaatacctaa acaacatcct gttataatat gagcagaaaa aaaaaataag 175680
tataattagg gaattattct tattcgctta ctattaagaa taattcagaa tcttatttag 175740
ttagaaacta tcataaagtg aataggactc atcgtcggat gaagattccg tttcagagat 175800
agtttctttt tcttcctcag aataatctgt tcctacaata gaatcggtgt catcctcaga 175860
aagagaagta tttaaatatg gactatctat agcaatatcc tcttctatct cgcaatcctc 175920
ctcctccatt tccatagtgt gtaggagaat atttttatca tcatgctcac ttcttttttt 175980
gttgaaagat gaaccgtcct caatacggtt catgttaagt tccttcatct tatgtataat 176040
ttccgtaatc cgtgatgttt ttgacatgta agatggtttt aaggttatat ccacaataac 176100
aggagaatct ctatcatttt catttgataa actttgatct ttgatttctt cgtctaaaat 176160
tcttgtcttt ttttgggtac tagatgaaat agaggaattc atattctgaa acgatatatc 176220
aaggggagct ggacgctttt ttccaattaa accgtttttc gagatactat gattagatga 176280
atgatcttta gccaagctgt ccttggatat actatagtta gatattttac ctttaaataa 176340
tattcttcta tacaagttat tcttaggtaa agaattagta tggattccta tatttttatc 176400
tgaaggagtg tccatatcgg agaacgtcct cttacgaata ttttgaccac gagccatttc 176460
atccactata ggcagtattt tggctggcta tggttctttg ttgtgacaat tctatgagat 176520
ttgattgcaa atcaattttt agttttaaat atattggtac ctaggacaaa gaaagtatat 176580
atagccaata attattccac taaattgatt tccagactga tgggtatgga gccatgttgt 176640
ctctgcagac gatcgcaaaa atggccgtag caacaaacac ctactccaag tatcactatc 176700
caatactgaa ggtctttggg ctgtggtgga aaaacaatac gctaaatggc cctattaaaa 176760
tatgtaacca ttgcaacaac ataatggtag gagaatatcc tatgtgttac aatcatggaa 176820
tgagtctgga tatagctttg attcgggcag taaaggagcg taatatatcc ttagtccagc 176880
ttttcaccga atggggggga aatattgact atggggcact ttgtgctaac actccatcta 176940
tgcaaagatt atgtaaaagt ttgggagcca aaccaccaaa gggccgaatg tatatggatg 177000
ctcttataca tctttcagat accttgaatg ataatgatct gattaggggg tatgagattt 177060
ttgatgataa tagcgtgttg gattgtgtca atctcatacg actcaaaata atgcttacct 177120
tgaaggcccg tatacctctc atggaacaac tagaccaaat tgccttaaaa caacttctgc 177180
agcgatactg gtatgccatg gctgtacaac acaacttaac aatcgctatc cactattttg 177240
ataatcatat tcctaatata aagccattta gtctgcgctg tgctttgtat tttaatgatc 177300
cctttaaaat ccatgatgct tgcagaactg taaatatgga tcctaatgag atgatgaaca 177360
ttgcttgtca acaggattta aactttcaaa gcatttacta ttgttatctt ttaggggctg 177420
atattaatca ggctatgcta atgtctttaa agtatggtca tctttctaat atgtggtttt 177480
gcatagattt gggggcggat gcctttaaag aggcaggggc gcttgctgag aaaaaaaata 177540
aaagagtgtt acaacacata ttaggtctta atatctttaa gcgagagttg attcccccct 177600
gtaaagatcc tgatccttat caaatccaaa ttctgttaaa aaactacatt ctaaaaaatg 177660
tctcaactgt ttttacatat tattgccagt agccattgtt tatatcagaa aataacccat 177720
ttgtttatct ttttttgtgg ggcaaccatt aagacccgac gcaaaaaaag attaatcttt 177780
tatcagatac ctaaaacgtt ctataaggga gtctatgaga tggatcatat tttgatggtc 177840
atagtaagaa gcaagctttt tggcgaaaac aacggagtta aagaatttaa cccgctcatg 177900
tttggatagg acttttaaca gcgagccaaa acagtattta aaaatttggc aatagttttt 177960
ttgggatgca ataaacaaac acttgatcag tgcccgcttc actttctgat cagacatgtt 178020
tgccgcataa caggcctttt taaacttagt aatataatta tgttccgcaa gcaccattaa 178080
caagggaacg atgggaagct gcttttcttg gtgaaattta cgtaaatatt cgatggccac 178140
cgcttggacg actgtgtaat ttactaagtt agaaatgata gctttcatgg ttgtaaaaat 178200
atacatagga ttttcttttt ctgtatacag tttgaaaagc ttatgattac gtgaaatgat 178260
ggccattttt aatacaagat ggtatagtgt atctttaggt aaaaatgcct tgcaagccgc 178320
gatgatgtcg atgttgtctc catgaacagc gatagaaact aatgtttcca atctaaatgt 178380
ttttatctgc attaatagaa gaatgcagtc aatgttatta tacttaataa tactgtaata 178440
caccgaatca atgaccgtca tctgagaatc aagctgactt attagtaaat ttaacgtttt 178500
tttggaggca tgacctttga tcgcggcact aagtgcacac agtatagcaa aattgttaaa 178560
tacattttga tttaggagaa ggagtaatat tttccttcgg ttatagtacg cagcatctgt 178620
gatgattatt ggccgataaa tgttaaaatg tgttaacagc tttttaaaaa aacggaagta 178680
atttttttgg atcgctgttt gcatcatcga aataatgaga taatcagggt atataatggg 178740
taggtcacat gctacctcta acaaagaata gtcgcccaat ctaaaggctg tgttgaaaag 178800
cgtactatca tcatacgtat cgagtacccc tgctgttaca aaccaagcga taagatgaat 178860
gtgccgttcc ttgcaagcta tcgcaaatag ggagtttcct atggaatgtc gaataatgta 178920
ctccctattt ttttccaaaa tgtttggaaa attgtatagc gttgcggcat acagtagaca 178980
ctccattctg gcgttataat ttttactttt acatatgaat aggtggaaga actcgaataa 179040
ttcttgagaa cttgttaaat gcataatatg gtgatatttt ggtgtcgtta aatggtatga 179100
gaaaatgcat tctaatacat cttttcggtt atgctttagc gcctgagcta aggcatattc 179160
aggctcgacc cataggacta gtgtttctat aattgagata ttcgcctgct ttgccagggc 179220
atactttaag acgctccggt tagaaaaaat gttgttatga agatggataa ccgtatccat 179280
ttttacgatg ggaccattcc agtatagtcc taaatgctgt agcagatctt ttgttagttg 179340
tgaagcgttc tcgggtgtca tataaatatg ttgcagggct tttttctgta aggagaacat 179400
ttcgtcgtaa tcgtacaaaa aaaaattaaa atttgggcat ggatgattca aacataacaa 179460
aatcaagatt ttataacagt ttgcattaac ctatacatat atgcaagtaa atgagatatt 179520
atctatcata acgaatcaag ggatatttgt atatatcagg agtttctgaa ataaagatat 179580
gaagattatc atagtagtat ccatcaatca caatgcaact tcctttaagg cataatttag 179640
taaactcagc actcccatct tctggatgct ttacaactaa cattaaaaac tcctcagtca 179700
tattatctgt aataaaataa gatcctcctg gagccatttg tagcatgtct cttattccta 179760
caaaatcttt tttgggatgg taaaaactca gcagtttcaa actctttttt agtttttttt 179820
cctggtattt aagccatttg ttataaaaca gttttcttat gaaaatgcat ttgaaaatat 179880
tgggaatgtt taaccatgct tcttccgagc acatctccag atacttactt tctttgtttc 179940
ccatgtctaa tttattgctc actaagttag taatgaatct attttaataa tctactttac 180000
taatctatct taataaccta tcttataatc tatcttaata acctaattat aacctattta 180060
taattggcta atgctgccgg catttcatgc ctatctaaac aactcctact aagcaatcta 180120
ctattacata tatagattca ctttttatat ttgtaaatca tgagaattat aaaatcatta 180180
ctcattttta ttgtaaatta gtgggtattt gtaaaaatct tcaaacgttt taagatagtt 180240
ttctagagag aagtaatctt tgccatcaat atataatgct tttcctttaa actccagttt 180300
tgctatgttt agtgagccgt ttctagatct ttttgggcaa taaatagatt ttcattggtt 180360
gcatcgtccg taagcagaaa ggtaccacta ggcacgttaa aaaacatacg ttctatttca 180420
tggtcggatt tttgagaata gaaaaaatct aattttttaa tccgcgttaa ctctttttta 180480
tcaatctttc cagactgttt tatatatact ttattgcaaa tcttacaatc ctctatggct 180540
tcattatact tattttgctt atcctctatt gacatgtccg tatttgatag gtaacttccg 180600
ttaaggcggt tccccatggt tttagataga tttttaattc agttgtatac ttttattatg 180660
aggctaaaat atagaagttt gatcctaaaa aaataaaaag attttgtaca tttatttatg 180720
gtttatagcg gtatagaggc cgataaaagg tatccgggta gtctcctatg atatcgtcaa 180780
ttttggtata ataacagttg ttatggtagt attgtccaaa ccgagtatgt atgcgccggt 180840
gaagcgtccg cccgctaatg gtacagttcc aggttaagac aatcatatca cacccaaaaa 180900
gagaggaaac agcataggtg cccaaaggtt cattatataa catacgccgc atatatttta 180960
gttttttttc tccatggtaa taatcacagg ttttcatgtc ctgcttaata ggatgattcc 181020
ccatgtatga taatatataa taaatttagt ttttagcttt ttcaaaaaat tgggcgctcg 181080
aaactaaatt ttccttatca cagcgtttgg agaaagcgta tttaaagata tatcttcttc 181140
taacaagact gcaaaaaaaa tcttacccct tatttttata atgttcatca tagcgtttga 181200
agatatcaga aggtgccagg ttttataaaa atatccttta ggatttataa cgatacaagg 181260
gtctataaaa tatatgcggg tataatctta taaaatcatc gattttttca taatattctc 181320
cgtttataca ataaagatca taacagatat tgatgcgtag atgcattatt cgcgtgttcg 181380
ttgggcagct aaaggatatc acaacgtagt tttttttaag aaaagacgaa actacataag 181440
tccctaaggg ttcattgaat agtaaacgcc atatttgttt taaattttgt tgttcaccat 181500
agtagtattc gcactttttc aagtcttttt taataagcct attccccatg tatgcttata 181560
aataaaaatt tagaaatgtg ctatattatt tgttgatgaa tcatgaacac gtcttatatg 181620
ttgatatgtt actttaaaaa catttgtatt ttcaacagac gcgttctatt cttattaaga 181680
atgatgccgt ctttatttta aaccttggtt taaaatttaa agaagtattt ataaactata 181740
atcatgggaa ctttttcagt aactgcctct gcaaaaagtg acgatgctgt ttgtaagtat 181800
ttagaagaac caatagatga aaattacaga aacatattaa gaaatgagca tgttaaaaaa 181860
aatttaaatg aggctctgaa tcgacatatt actacctata atccagtagt tgattggtgt 181920
aataactatt caacattttc atctcaggat ttcgatgaat ataaaattta tatacatagc 181980
gatcttatgg atggacgacc tcgtccaaaa aaaacatggt gtgtcatcat gtaatgtttg 182040
ttagttttat ataaacgcaa aaatattctt ctaggagatg ttgatatact acctattgaa 182100
ttcaatatat taaagtacat ttctggctat tcccattacg gtattattat tactattttt 182160
aagagctaga tgtggattta agtaataata acattctccc gttcctccta gagacacctc 182220
atcaaattcc catcctatgc aacctttatg ttgtaaacat aatgattgac agcattcatc 182280
ttcttttgac caagtcgtcc aaatcctacc aagatctata cgtgtttttc caaatggaga 182340
ttgaagatca gcagtagtgg cattaaacct ataaaaacca ggtgcataat cacatgaacg 182400
gatcgtagga tctaatttaa tatcttttat atcttgtttt actgcttcta gacaactttt 182460
atcagtacat gttccacgta cacagtggtg tcctttatcc ttacaatccg tatctgtctt 182520
acattttttt ttcggcggtt tatgtttcag atggtaaaaa cccagtatta aaataatcac 182580
aagaataatt cctataagta cttgaacaac aggataaaac attttaatat taaatatatt 182640
ttttaattaa atgaatagat ttaatccaag tagtattaaa attttttaga aatagtgttc 182700
tacaaataat gaaatgaatg gtccaaaaaa aataaggtgt acaataatgt aatatattgt 182760
taggctaagt aaatttaata ttttaaagta tttggaaaaa tattttttaa catatgatgt 182820
ctaggaatat tttttagaca tttaaaacca tatagttact ttatttatta cactgaactt 182880
gaaaagactt attacctaaa atattaatag atgaagtaat attgtgtaat tgagtccata 182940
acatgggtgg gaaacaaaaa tctcgtaata tgaaaaataa acatcctaaa aagagtgcaa 183000
ttgttataag tttatgtaac tttattttaa agtaagaata taaaaatatg agtacaagag 183060
gaataggggc cattactaac attggctcca acatcctgtt gtctacaaaa aaaaatattt 183120
tttttagcaa aaaaaaatcc atggaaggat attaatacac ataattattt gacatcacat 183180
tagtgtactt accaaatagt aatatacaac catcctaata ttcaccttta tgaaatgatc 183240
ccaacctata cggtaaaata gtataggttt taataaagaa aaaagatatt ctgtggtttt 183300
tatttttgta tagtgtgtga atacaaaata aaatcccaaa ttttaacctt ttcttttttt 183360
ttctatacag gatgttagaa atagtattgg caacgctgct aggcgacctg cagcggctcc 183420
gggttcttac ccctcagcag cgggcagttg ccttctttcg agccaatact aaggagctag 183480
aggacttctt atgctcagat gggcagtctg aggaggtact gtctggcccc cttcttaacc 183540
gtctactaga accctcaggc cctcttgata ttttaaccgg atatcaccta tttcgtcaga 183600
atcccaaggc aggtcagttg cgcggccttg aggtcaagat gcttgaacgg ttatacgatg 183660
ctaatattta caatatactg tctcggctgc ggcctgaaaa agttcgcaac aaggctattg 183720
agctatactg ggttttccga gctatccata tttgtcatgc tcctttagtt ttagatattg 183780
tacgatatga ggaaccggac tttgctgaac tggcctttat ttgtgctgct tactttggtg 183840
aacctcaggt aatgtatttg ctctacaaat atatgcctct gacccgcgca gttcttacgg 183900
atgccatccg gataagtctt gagagcaaca accaggtagg gatttgctat gcttacttga 183960
tgggaggcag cctcaaggga ctagtctccg ccccactgcg taaacgtctg cgcgccaaac 184020
tacgctcgca gcgcaaaaag aaggacgttc tttcacccca cgacttctta ctgctgctcc 184080
agtagctttt tttgccgcag gagcaccgcg gataggagct cctccacgct cgcgatccgg 184140
cgctggaagc ggaaccgatc gaccgccacc tgctcccagg gacccttgcg ctcgatgtcg 184200
tcggcttccc acacctcgac ggctgtggca aaatggacat gcttcgcgtc gttcgtccgt 184260
tttttgcgcc gcctccccat tattcttcct gtaagattag tgtttaatac ctataataac 184320
ataattttaa gatttaatat accaaaactt aaactatttt tgtatagtaa ctattagcat 184380
gtctacacat gattgttctc taaaagagaa accggttgat atgaacgata tatctgagaa 184440
atcagttgtc gtggataatg cacccgagaa accagctgga gcgaatcata tacctgagaa 184500
gtcggcccgc gaaatgacat catcagaatg gattgctgaa tattggaaag gtataaaacg 184560
tggaaatgac gtgccatgtt gttgtccaag aaaaatgacc agtgcagaca aaaagttttc 184620
agtatttggt aagggatccc taatgcgctc catccagaag aataattaaa aaaaatattt 184680
tttttagcaa gtttttaaac tatttaaata aatgtggtaa aaaaattcac ataataatta 184740
aagtgaacgt gttagaatta atattttttt ataatcggat ataatatcca ttaaatcaat 184800
aaatgatagt gttgctacca cactaaacaa taacaaacag aaacgcacga tacctttcct 184860
catgatttat aatagcgtgt tatctaaaga tttttttgaa aaaaatatta aattttagtt 184920
gattattttt ttcagttaca acattgcttt agaaaaaata cctaattact acatagcaaa 184980
taaagcgagc gcattgttac aaacaacatt tttttgcgcc tggatactcc tatatatgag 185040
aactataata cggtatatta atcctattac caacattgtc aataatagta tgtaggcaat 185100
gacatacttt aaataccaaa tatccatggt tatttctaaa aatcttgaaa aaacgttaaa 185160
ttttagatcg gtcacctacg acagtaatac taattttaat aattgatgac tgaaatcata 185220
atataatgcc gtgcgaaaaa taattatttt tcggttaaag ataccattac ataaaaaata 185280
tgccatctac tctacaagtg cttgctaaaa aggtattggc cttaggggag cataaagaaa 185340
atgaacatat atctagagaa tattattatc atatattaaa gtgttgcggt ttatggtggc 185400
atgaagctcc gattatactt tgttatgatg ggagtgagca aatgatgata aagactccaa 185460
tctttgaaga aggcatatta cttaatactg cattaatgaa agctgtacag gagaataatt 185520
atgaattaat aaagttgttt actgaatggg gagcaaacat caattatgga ttaatttcca 185580
ttaataccga gcatgcccgg gatctatgtc gaaaattagg agctaaagaa atgcttgaag 185640
gaaatgaatt tatacaaatt atattcaaaa cattagatga taccaccagt agtaatataa 185700
ttttatgtca tgaattattc accaacaatc ctcttttaga gaatgtaaat atgggggaaa 185760
tgaggatgat aatttattgg aggatgaaaa atttaacgaa cctattatta aataatgact 185820
ctattagtga aatattaact aaattctggt atggtatagc agtaaaatat aatcttaagg 185880
atgcgatcca atatttttac cagagattca tggacttcaa cgagtggcga gtaacatgtg 185940
ctctttcttt taataatgtg aatgatcttc ataagatgta tataacagag aaggttcata 186000
tgaataatga cgaaatgatg aatctagcct gcagcattca agacagaaat ttatcaacca 186060
tttactattg ttttctattg gggggctaac atcaatcaag caatgttaac ctcagtatta 186120
aattataata tttttaactt attcttttgt atagacttag gggctgatgc ctttgaagag 186180
ggtaagaccc tggcgaaaca aaaggggtat aatgaaatag tggaaatctt atcattagat 186240
atcatttata gtccaaatac tgacttctca tcaaaaatag aacctgaaca tattagttct 186300
ttgttaaaaa acttttatcc aaaaaatctg ttcgcttttg atcgttgcaa ccccggttta 186360
tattattctt agaggaccgc tacaaaaatt attttttttt cttgatcaaa gctccaaaat 186420
aattattaga ttaaagtcgc ctatagcagc agcccactcc aaaaaaagta ttttatagta 186480
caaaaaacac gaaaaatagt ttgcggccgg cggcaaacta tttgttgttg tctaaaactt 186540
aatgtttttt taatattttt aaatgcaacc atggattgtt ggactatcag ggagaagaac 186600
tatagctaca tcatattgtc aatactggta atactattaa tatggtatct tatacttaac 186660
tattgtcgat cgaaaaaaaa tgcagttaca aacaacatgc cgccaccata cacggtgtca 186720
agtagctgtt ctcaataata gggttgattg acgctcttcg taataatatg ttgattgacg 186780
catcataaaa tgctgtggtt gattaatatg ttgattgtcg cctactttat tatataagta 186840
atgatttttg tataaaatac gggtttgtga gggctttatt ttttcttatt agaacaaagc 186900
atgcaattta aggcctacag caagagtaat ttaacaccta caacagtaat tttaaggtca 186960
gtaataatgt ttaattaagg cctgaccact aaaacttaaa cgattttgta aaaaaaaatg 187020
tctactccac tttctctaca gactcttgtt aaaaaagtgc tggccacaca gcacatatct 187080
aaagaacact actttatttt gaaatattgt ggtttatggt ggcatgaagc gccgattacg 187140
atttgcattg atgaggatag ccaaatattg ataaaatcgg caagcttcaa agaaggctta 187200
tctttagata tcgcattaat gaaagtcgtg caagaaaata accatgattt aatagagttg 187260
tttaccaagt ggggtgcaga tatcaactct agcttagtta ctgttaatac ggagtatacc 187320
cggaaccttt gtcagaaatt aggcgcaaag gaagctttga atgaaaggga tattttacaa 187380
atattttata aaacacgtca tcttaaaact agcagtaata ttattttata taatgaattg 187440
ttttctaata atctcctttt ccaaaatata gagagattga gtttaatagt ttataggggc 187500
ttgaaaaact tatcaatcaa ctttatattg gatgatattt catttagcga aatgttaact 187560
agatactggt atagtatggc gatattatat aaccttactg aagccatcca atatttttat 187620
caacgatata ggcattttaa agattggcgg cttatatgtg ggctttcttt taacaatttg 187680
tctgaccttc atgaagtata taacttagag aagacggata tagacattga tgaaatgatg 187740
aagttgacct gtagtacgta tgatggtaat tattcgacta tttattattg ttttatgttg 187800
ggggctgaca tcaatcgggc aatgttaacc tcggtaataa actttcatat tggtaacttg 187860
ttcctttgta tagatttagg agctgatgct ttcgaagaca gcatggaact agcaaaacaa 187920
aagaataata atatattagt agaaatatta tcatttaaaa attattatag ttcaaatacc 187980
tctcttttat caataaaaac gacagatccg gaaaaaatta atgccttatt agatgaagaa 188040
aagtatgagt caaaaaatat gttaatgtat gaagaattat ctcattgata caaaattatt 188100
ttttataaca gaactctctg atggtgacaa atctccgata ggaatatatg acgtaacata 188160
attatttttt tcgcccagaa aaaaattata aatgttatta ttgccagcac ttttatcaac 188220
tatacgtaca aaaaggtgtt gaccaaaaaa ataatttttt ttcttgatca aagtatgtaa 188280
acgcccgctt acagcaagga tcttaagtga gagccattaa attttattga tagctgcttg 188340
ccaccagtag aatacggcca aaccacctaa caggaaatac aaggcggccc ttcggccaat 188400
aaggtggata aaaatcacgc ataagacggt tgtaacatag cactttagtg cgaatatcag 188460
gaatgccaat agcatgtaga taaggcacca aacatcgcag ctatacatgg ctaaagatca 188520
accagaaaag gtttaaattt taacgccggc ccaaaactta aacttttttt gatattttta 188580
agtgcagcca tggattggtc cggccatagg atgacctatg cctacgtggc attctcattg 188640
atggcaatag caataatatg gtatattcta cttatctatt gccgatcgaa aaaaaatgtt 188700
gttacaagcg gtaatacgct cgctttagcg ccaatatcgc atatgtgaaa aatgttcgcc 188760
gaaaaaaaca ttaaaattta gaaccgccgc ggcatctcag gggcggcaac attttttttt 188820
atatggatat tgtcacacac cacctcatct atgacgcaat atattactgc taatatcagg 188880
ttccccaata gtatgtagag aaaccacaca agatagatat tcatggcgat ttttgacgaa 188940
aaaacattaa gttttagctt ctttgacgcc tgtgtactaa taatgtttaa cgcctgtagt 189000
ataataattg atacctacag cagtaattga tacctacggc gataatgtct ctctggccgc 189060
cccaaaaaaa agtatttacg gtagggttta ttaccggcgg cgtaacacca gttatggtca 189120
attttgtctg gcccgccgcc cagccgcaaa aaaaaatcaa ttacaaccgc aaaaaaaaat 189180
atttccggcc gcggcgtttc aaaaaataat ctttgcgaaa taattccgca tcttgtgaaa 189240
tgaacgccta cagtaataat tttaatcttt gacacctaca gcagtagtaa taattttaat 189300
ctttaacgcc tgcagcagta ctaatatttt taatctttaa cgcctacagc agtagtaata 189360
attttaatgt ttaacgccta cagcagtagt aat 189393

Claims (7)

1.通过在基因Ⅱ型非洲猪瘟病毒中缺失基因片段制备减毒非洲猪瘟病毒株的应用，其特征在于，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示；所述基因片段为ASFV CN/GS/2018分离株全长序列的第184062-184456位。
2.通过在基因Ⅱ型非洲猪瘟病毒中缺失基因片段制备非洲猪瘟疫苗的应用，其特征在于，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示；所述基因片段为ASFV CN/GS/2018分离株全长序列的第184062-184456位。
3.一种基因缺失减毒非洲猪瘟病毒株，其特征在于，所述基因缺失减毒非洲猪瘟病毒株为基因片段缺失的基因Ⅱ型非洲猪病毒，所述基因Ⅱ型非洲猪瘟病毒为ASFV CN/GS/2018分离株，所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示；所述基因片段为ASFV CN/GS/2018分离株全长序列的第184062-184456位。
4.一种非洲猪瘟减毒疫苗，其特征在于，所述非洲猪瘟减毒疫苗包括权利要求3所述的基因缺失减毒非洲猪瘟病毒株。
5.一种制备权利要求3所述的基因缺失减毒非洲猪瘟病毒株的方法，其特征在于，所述的方法是通过基因工程手段将全基因序列如SEQ ID NO.20所示的ASFV CN/GS/2018分离株的第184062-184456位核苷酸序列缺失。
6.如权利要求5所述的方法，其特征在于，所述方法为同源重组技术。
7.一种制备权利要求5-6任一所述的方法，其特征在于，所述方法包括如下步骤：
（1）pX330载体优化；通过ClonExpress II一步法克隆法去除pX330载体中Cas9酶两端的核定位信号NLS，命名为pX330ΔN；
（2）设计靶向寡核苷酸181/UK-gRNA-L和181/UK-gRNA-R，将寡核苷酸配对插入pX330-ΔN载体中，制备阳性克隆质粒pX330ΔN-181/UKL和pX330ΔN-181/UKR；其中，所述181/UK-gRNA-L的核苷酸序列如SEQ ID NO.8所示，181/UK-gRNA-R的核苷酸序列如SEQ ID NO.9所示；
（3）设计MGF360-18R基因、DP71L基因和DP96R基因组合片段的上下游序列作为同源重组臂，并同时克隆入pUC19载体中，获得重组转移载体p181/UKLR；
（4）在重组转移载体p181/UKLR左、右臂基因序列中间插入eGFP筛选表达盒基因片段p72-eGFP-SV40poly，获得同源重组转移载体p181/UKLR-eGFP；
（5）将同源重组转移载体p181/UKLR-eGFP、pX330ΔN-181/UKL、pX330ΔN-181/UKR和JetPEI®-Macrophage DNA转染试剂充分混匀，共转染至取自2-4月龄健康SPF巴马小型猪的BMDM细胞，直接感染ASFV CN/GS/2018分离株；所述ASFV CN/GS/2018分离株的全基因序列如SEQ ID NO.20所示；
（6）病毒株筛选：利用181/UK-check-F/R引物对重组病毒株筛选，获得基因片段缺失的减毒非洲猪瘟病毒株Δ181/UK，所述181/UK-check-F/R引物对的核苷酸序列如SEQ IDNO.16-17所示。
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