KR102066898B1 - 신규한 장알균 특이 박테리오파지 ef5 및 이를 포함하는 항균 조성물 - Google Patents

신규한 장알균 특이 박테리오파지 ef5 및 이를 포함하는 항균 조성물 Download PDF

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KR102066898B1
KR102066898B1 KR1020170169631A KR20170169631A KR102066898B1 KR 102066898 B1 KR102066898 B1 KR 102066898B1 KR 1020170169631 A KR1020170169631 A KR 1020170169631A KR 20170169631 A KR20170169631 A KR 20170169631A KR 102066898 B1 KR102066898 B1 KR 102066898B1
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present
enterococci
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enterococcus faecalis
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연영은
김영주
한범구
김현일
박철세
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주식회사 옵티팜
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • C12N2795/00011—Details
    • C12N2795/00032—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent

Abstract

본 발명은 장알균, 특히, 엔테로코커스 패칼리스(Enterococcus faecalis)에 특이적인 사멸능을 갖는 신규 박테리오파지 EF5, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 장알균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다. 본 발명의 박테리오파지 EF5를 이용한 장알균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 장알균, 특히, 엔테로코커스 패칼리스에 대한 특이성이 매우 높고, 상기 박테리오파지 EF5는 상기 장알균에 감염하여 장알균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 EF5는 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 EF5는 장알균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.

Description

신규한 장알균 특이 박테리오파지 EF5 및 이를 포함하는 항균 조성물{Novel Enterococcus faecalis specific bacteriophage EF5 and antibacterial composition comprising the same}
본 발명은 장알균, 특히, 엔테로코커스 패칼리스(Enterococcus faecalis)에 특이적인 사멸능을 갖는 신규 박테리오파지 EF5, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 장알균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.
일반적으로 엔테로코커스(Enterococcus)속 균주는 장알균이라고 부르며 그람양성이다. 대표종은 엔테로코커스 패칼리스(Enterococcus faecalis)와 엔테로코커스 패시움(Enterococcus faecium)이 있다. 상기 균주는 사람이나 온혈 동물의 장관 내에서 상재하며 분변을 통해 배출된다. 일반적으로 병원성은 비교적 낮지만 드물게 아급성 심내막염이나 요로감염의 원인이 된다거나, 치주감염, 장관계 감염, 창상감염 및 패혈증 등 원내감염의 주요 원인균으로 알려져 있다. 엔테로코커스 패칼리스 감염의 특징 중 하나는 치아 뿌리, 요도뇨관, 요도 스텐트, 심장 판막 등에 바이오필름을 형성한다는 것이다. 바이오필름은 미생물에 의해 생성된 막 형태의 구조물로, 바이오필름으로 증식하는 미생물은 동일 미생물의 플랑크톤 형태보다 항생제와 숙주방어에 대한 저항성이 높다. 이와 같은 바이오필름 감염을 해소하는데 많은 어려움이 있으며, 만성 또는 재발성 감염이 흔히 일어난다. 또한 엔테로코커스 속 균주는 드물게는 감염형 식중독을 일으키기도 하는데 식품이나 공공용수 등의 오염지표로서 사용되는 세균이다. 특히 반코마이신에 내성을 나타내는 장알균은 다른 병원성 세균에 비해 상대적으로 연구가 많이 되지 않고, 병원성에 대한 중요도가 낮았으나 최근 다양한 장관계, 피부, 호흡기 감염에 있어 원인세균 또는 복합 감염의 원인으로 주목받고 있다. 이와 관련하여, 엔테로코커스 패칼리스는 반코마이신에 강력한 항생제 내성기전을 가지는 것으로 알려졌으며, 반코마이신 내성 장알균(VRE)은 1986년 처음 보고되었는데, 병원감염의 중요한 원인균으로서 치료가 어려워 감염 관리의 주요 대상 세균이다. 전에 반코마이신을 투여 받은 적이 있거나 다른 항생제라도 오랫동안 사용한 사람은 이 균에 감염될 수 있다. 또한, 반코마이신 내성 유전자가 황색포도상구균 같은 다른 그람 양성 균으로 전이될 가능성 때문에 더욱 위험하며, 특히 내성 유전자인 vanA가 이동성 전이인자에 의해 빠르게 전이됨으로써 퍼져나가는 것으로 알려졌다. 최근에는 vanB, vanD, vanE, vanG 같은 많은 내성 유전자가 발견되었으며 이 중 vanB는 vanA처럼 플라스미드에 존재하여 빠르게 전이될 수 있는 내성 유전자다. 이러한 항생제에 대한 내성은 엔테로코커스 패칼리스의 바이오필름 형성능으로 인하여 더욱 강화되고 이에 따라 균의 제거가 어려워지면서 효과적인 치료제 개발이 요구되고 있다.
한편, 박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다. 따라서 최근 세균성 질환의 대처 방안으로 박테리오파지(bacteriophage)의 활용이 크게 주목을 받고 있다. 특히 2000년 이후에 항생제 내성균의 증가로 인하여 기존 항생제의 한계성이 나타나고, 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다.
그러나 현재까지 장알균에 의한 감염증을 예방 또는 치료하기 위한 신규한 박테리오파지에 대한 연구는 여전히 부족한 실정이며, 장알균에 특이적인 용균활성을 갖는 박테리오파지 및 이의 응용에 대한 기술 개발이 필요하다.
이에 본 발명자들은 장알균에 특이적인 용균활성을 갖는 박테리오파지를 분리하고 분리된 박테리오파지의 형태적 및 유전적 특성을 분석하여 상기 박테리오파지가 장알균, 특히, 엔테로코커스 패칼리스를 선택적으로 사멸시킬 수 있음을 확인함으로써 본 발명을 완성하였다.
본 발명의 목적은 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF5를 제공하는 것이다.
본 발명의 다른 목적은 상기 박테리오파지 EF5를 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 EF5를 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.
상기 목적을 달성하기 위하여, 본 발명은 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF5를 제공한다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 항생용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 사료 첨가용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 소독제를 제공한다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 세척제를 제공한다.
또한, 본 발명은 상기 박테리오파지 EF5를 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 EF5를 이용한 장알균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 장알균, 특히, 엔테로코커스 패칼리스에 대한 특이성이 매우 높고, 상기 박테리오파지 EF5는 상기 장알균에 감염하여 장알균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 EF5는 사람, 동물, 식물 등 세균외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 EF5는 장알균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
도 1은 항생제 내성을 가지는 엔테로코커스 패칼리스에 대해 특이적인 박테리오파지의 스크리닝 결과를 나타낸 도이다.
도 2는 박테리오파지 EF5의 엔테로코커스 패칼리스에 대한 용균반 형성 활성을 확인한 결과를 나타낸 도이다.
도 4는 전자 현미경을 통해 박테리오파지 EF5의 형태학적 특성을 확인한 결과를 나타낸 도이다.
도 5는 박테리오파지 EF5의 단백질 패턴을 SDS-PAGE를 통해 확인한 결과를 나타낸 도이다.
도 6은 박테리오파지 EF5의 pH 안정성을 확인한 결과를 나타낸 도이다.
도 7은 박테리오파지 EF5의 열 안정성을 확인한 결과를 나타낸 도이다.
이하, 본 발명에 대해 상세히 설명한다.
본 발명은 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF5를 제공한다.
본 발명에서, 용어 박테리오파지(bacteriophage)는 특정 세균에 감염하여 당해 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.
본 발명에서, 상기 장알균은 바람직하게는 엔테로코커스 패칼리스(Enterococcus faecalis)이다.
상기 엔테로코커스 패칼리스는 항생제에 내성을 갖는 다제 내성균일 수 있으며, 예를 들어, 반코마이신, 아미노글리코사이드, 아즈트레오남, 세팔로스포린, 옥사실린, 설파메타졸 등 널리 사용되는 항생제에 대해 내성을 갖는다.
본 발명의 박테리오파지 EF5는 정이십면체의 머리를 가지며 긴 꼬리가 있는 사이포비리대(Siphoviridae)에 속한다. 보다 구체적으로 박테리오파지 EF5는 머리 부분이 약 91.6-100 nm의 크기이며 꼬리의 길이는 약 420-520 nm이다.
상기 박테리오파지 EF5의 단백질 패턴 분석 결과 70 kDa, 50 kDa, 36 kDa, 32 kDa, 24 kDa, 그리고 17kDa 크기의 단백질을 포함함을 확인하였으며, 박테리오파지 EF5는 열 및 pH에 대한 안정성이 우수한 특성을 가지고 있다.
본 발명의 박테리오파지 EF5는 서열번호 1로 표시되는 염기서열을 전체 유전자의 전체 또는 일부로서 포함할 수 있다. 또한, 본 발명의 박테리오파지 EF5는 서열번호 1로 표시되는 염기서열, 및 상기 염기서열의 기능적 동등물로 이루어질 수 있다. 상기 기능적 동등물이란 염기서열의 변형, 치환의 결과, 상기 서열번호 1로 표시되는 염기서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 염기서열과 실질적으로 동질의 생리활성을 나타내는 서열을 의미한다.
상기와 같은 장알균 특이적 용균활성, 내산성, 내열성은 본 발명의 박테리오파지 EF5를 장알균에 의한 감염성 질병의 예방 및 치료용 조성물, 및 상기 박테리오파지 EF5를 유효성분으로 포함하는 다양한 제품에 적용함에 있어, 다양한 온도 및 pH 범위의 적용이 가능하게 한다.
본 발명의 박테리오파지 EF5는 도축장, 도계장 및 농장 부근의 하수로부터 시료를 채취하여 분리한 박테리오파지로서, 본 발명자는 이를 박테리오파지 EF5로 명명하고 2016년 9월 30일에 한국미생물보존센터에 수탁번호 KFCC11693P로 기탁하였다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물을 제공한다.
본 발명의 박테리오파지 EF5는 장알균에 특이적인 사멸능을 가지므로, 장알균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 사용될 수 있다. 보다 구체적으로, 본 발명의 박테리오파지 EF5는 엔테로코커스 패칼리스에 대한 특이적인 사멸능을 가지므로, 상기 균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 사용될 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서, 장알균에 의한 감염성 질병은 요로감염, 창상감염, 균혈증, 심내막염, 수막염, 혈류감염, 장관계감염, 치주염, 치은염, 농양, 균혈증 및 패혈증 등을 포함하나, 이에 제한되는 것은 아니며, 장알균에 의한 2차 감염을 포함한다.
본 발명의 약학적 조성물은 1 × 103 내지 1 × 1010 PFU/mL의 박테리오파지를 포함하며, 바람직하게는 1 × 106 내지 1 × 109 PFU/mL의 박테리오파지를 포함한다. 본 발명에 사용된 용어, PFU (plaque forming unit)는 박테리오파지가 플라크를 형성하는 것을 수치화한 단위이다.
본 발명의 용어 예방이란 조성물의 투여로 질병을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.
본 발명의 용어 치료란 조성물의 투여로 상기 질병의 증세가 호전되거나 상기 질병의 억제 또는 경감 및 이롭게 변경되는 모든 행위를 의미한다.
본 발명의 약학적 조성물은 약학적으로 허용 가능한 담체를 추가로 포함할 수 있다.
본 발명에서 용어, 약학적으로 허용 가능한 담체란 생물체를 자극하지 않고 투여 화합물의 생물학적 활성 및 특성을 저해하지 않는 담체 또는 희석제를 의미한다. 액상 용액으로 제제화되는 조성물에 있어서 허용되는 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로오스 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.
본 발명의 약학적 조성물은 질환 부위에의 도포 또는 분무하는 방법으로 이용할 수 있으며, 그 밖에 비강 분무, 경구 투여 또는 비경구 투여를 통해 투여할 수도 있으며, 비경구 투여의 경우 정맥 내 투여, 복강 내 투여, 근육 내 투여, 피하 투여 또는 국부 투여를 이용하여 투여할 수 도 있다.
상기 비강 분무의 경우 일정량의 박테리오파지 EF5를 물에 희석하여 분무기나 분무 시스템을 통해 비강으로 흡수 되도록 할 수 있다. 상기 비강 분무를 위한 비강 분무제 또는 호흡기용 제형으로는 에어로졸제 등을 들 수 있다.
상기 비강 분무제 또는 호흡기용 제형은, 수분산된 농축물 또는 습윤 분말을 분산시키기 위한 추진제 및/또는 기타 첨가제를 추가로 포함할 수 있다.
본 발명의 약학적 조성물의 적합한 도포, 분무 및 투여량은 제제화 방법, 투여 방식, 대상이 되는 동물 및 환자의 연령, 체중, 성, 질병 증상의 정도, 음식, 투여시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사나 수의사는 목적하는 치료에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다.
본 발명의 약학적 조성물을 유효성분으로 포함하는 경구 투여용 제형으로는, 예를 들어 정제, 트로키제, 로렌지, 수용성 또는 유성현탁액, 조제분말 또는 과립, 에멀젼, 하드 또는 소프트 캡슐, 시럽 또는 엘릭시르제로 제제화할 수 있다. 정제 및 캡슐 등의 제형으로 제제화하기 위해, 락토오스, 사카로오스, 솔비톨, 만니톨, 전분, 아밀로펙틴, 셀룰로오스 또는 젤라틴과 같은 결합제, 디칼슘 포스페이트와 같은 부형제, 옥수수 전분 또는 고구마 전분과 같은 붕괴제, 스테아르산 마그네슘, 스테아르산 칼슘, 스테아릴푸마르산 나트륨 또는 폴리에틸렌글리콜 왁스와 같은 윤활유를 포함할 수 있으며, 캡슐 제형의 경우 상기 언급한 물질 외에도 지방유와 같은 액체 담체를 더 함유할 수 있다.
본 발명의 약학적 조성물을 유효성분으로 포함하는 비경구 투여용 제형으로는, 피하주사, 정맥주사 또는 근육 내 주사 등의 주사용 형태, 좌제 주입방식 또는 호흡기를 통하여 흡입이 가능하도록 하는 에어로졸제 등 스프레이용으로 제제화할 수 있다. 주사용 제형으로 제제화하기 위해서는 본 발명의 조성물을 안정제 또는 완충제와 함께 물에서 혼합하여 용액 또는 현탁액으로 제조하고, 이를 앰플 또는 바이알의 단위 투여용으로 제제화할 수 있다. 에어로졸제 등의 스프레이용으로 제형화하는 경우, 수분산된 농축물 또는 습윤 분말이 분산되도록 추진제 등이 첨가제와 함께 배합될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 항생용 조성물을 제공한다.
본 발명에서, 용어 항생용 조성물은 약제 형태로 개체에 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다.
본 발명의 박테리오파지 EF5는 기존 항생제에 비하여 장알균에 대한 특이성이 매우 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 사료 첨가용 조성물을 제공한다.
축산, 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제이다. 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞여 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있기 때문에 좀 더 자연 친화적이면서도 기존의 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 EF5를 이용할 수 있다.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가시켜 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조 상태일 수 있으며, 바람직하게는 건조된 분말형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량%, 바람직하게는 0.1 내지 2 중량%의 성분비로 혼합될 수 있다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.
본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해 효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus sp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에 (Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.
본 발명의 박테리오파지 EF5를 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.
본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.
또한, 본 발명은 박테리오파지 EF5를 포함하는, 음용수 첨가제를 제공한다.
본 발명의 음용수 첨가제는 상기 박테리오파지 EF5 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 장알균의 숫자를 감소시킬 수 있는 효과가 있다.
본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 소독제를 제공한다.
장알균에 특이적 사멸능을 갖는 본 발명의 박테리오파지 EF5를 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양계장, 축사 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF5를 포함하는, 세척제를 제공한다.
본 발명의 박테리오파지 EF5는 장알균에 특이적 사멸능을 가지므로, 장알균에 노출되었거나 노출될 가능성이 있는 개체의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF5를 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 EF5는 장알균에 특이적인 사멸능을 가지므로, 장알균에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다. 보다 구체적으로, 본 발명의 박테리오파지 EF5는 엔테로코커스 패칼리스에 대한 특이적인 사멸능을 가지므로, 상기 균에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다.
본 발명에서, 장알균에 의한 감염성 질병은 요로감염, 창상감염, 균혈증, 심내막염, 수막염, 혈류감염, 장관계감염, 치주염, 치은염, 농양, 균혈증 및 패혈증 등을 포함하나, 이에 제한되는 것은 아니며, 장알균에 의한 2차 감염을 포함한다.
본 발명에서, 용어 "개체"는 장알균에 의한 감염성 질병이 발병될 수 있는 모든 개체를 의미하는 것으로 인간을 포함한 포유동물, 가축류 또는 가금류를 포함하나, 이에 제한되는 것은 아니다.
상기 용어 "가축류"는 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.
상기 용어 "가금류"는, 가축 중 조류에 속하는 동물을 총칭하여 이르는 개념으로, 예를 들어, 닭, 오리, 꿩, 메추리, 타조, 거위 또는 칠면조 등을 포함하나, 이에 제한되는 것은 아니다.
본 발명의 방법에서 상기 박테리오파지 EF5 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다.
본 발명의 방법에서 상기 박테리오파지 EF5 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있으며, 구체적으로, 구강, 직장, 국소, 정맥 내, 복강 내, 근육 내, 동맥 내, 경피, 비측 내, 흡입 등을 통해 통상적인 방식으로 투여될 수 있다.
본 발명의 방법에 투여되는 상기 박테리오파지 EF5의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.
본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술분야에서 통상적으로 사용되는 의미를 갖는 것이다.
이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.
실시예 1: 신규한 박테리오파지의 분리 및 배양
1.1 박테리오파지의 스크리닝 및 분리
장알균에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 용균반 분석을 수행하였다. 시료는 박테리오파지가 존재할 것으로 예상되는 도축장, 도계장 및 농장 부근의 하수로부터 채취하였으며, 박테리오파지 분리에 사용된 반코마이신 내성 엔테로코커스 패칼리스는 아시아태평양감염재단으로부터 분양받은 것을 사용하였다.
구체적으로, 채취한 시료를 균질화 한 후 엔테로코커스 패칼리스와 함께 35℃에서 하룻밤 동안 진탕배양 하였다. 상기 배양액을 4,000 rpm으로 20분간 원심분리한 후, 상등액을 공극(pore) 크기가 0.45 μm인 필터를 이용하여 여과하여 시료 여과액을 얻었다. 상기 여과액을 이용하여 반점분석법을 통해 대상 시료에 엔테로코커스 패칼리스를 용균할 수 있는 박테리오파지가 존재하는지 스크리닝을 수행하였다. 그 결과를 도 1에 나타내었다.
다음으로 전배양된 엔테로코커스 패칼리스 150 μL를 취해서 멸균된 튜브에 넣고 3 mL의 BHI(Brain Heart Infusion) 탑 아가(top agar)((Brain infusion solids 12.5 g, Beef heart infusion solids 5.0 g, Proteose peptone 10.0 g, Glucose 2.0 g, Sodium chloride 5.0 g, Disodium phosphate 2.5 g/L) +0.6% agar)를 첨가하여 잘 혼합한 후 미리 만들어 둔 2% BHI 아가 플레이트에 중층하였다. 실온에서 30분간 방치한 후 상기 시료 여과액을 각각 10 μL씩 점적하고, 다시 실온에서 30분간 방치한 후 35℃에서 24시간 동안 배양하여 박테리오파지의 유무를 확인하였다.
박테리오파지의 존재를 확인한 시료로부터 박테리오파지를 순수 분리하기 위해서 소프트 아가 오버레이(soft agar overlay)법을 이용하였다. 박테리오파지가 존재하는 것으로 확인된 시료를 식염수로 적절히 희석하여 각 희석단의 시료 희석액 100 μL를 취하고, 대상 세균(엔테로코커스 패칼리스) 배양액 150 μL에 혼합한 후 0.6% BHI 탑 아가 3 mL을 첨가하여 잘 혼합한 뒤, 미리 만들어 둔 2% BHI 아가 플레이트에 중층하여 방치한 후 35℃에서 24시간 배양하였다. 배양한 각 플레이트에서 형성된 용균반을 확인한 후, 이를 선택하여 400 μL의 SM 완충액 (NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1M Tris-HCl (pH 7.5) 50 mL)에 현탁하고, 실온에서 4시간동안 방치하였다. 상기 박테리오파지액 100 μL를 0.6% BHI 탑 아가가 포함된 세균 배양액 500 μL와 혼합한 뒤 150 mm 지름의 BHI 플레이트에 분주하여 중층한 후 완전히 용균이 일어날 때까지 35℃에서 배양하였다. 배양이 종료된 후, BHI 플레이트에 15 mL의 SM 완충액을 가하고 실온에서 4시간 동안 천천히 교반하면서 박테리오파지 용액을 회수한 후 4,000 rpm으로 20분간 원심분리하여 상등액만 취하고 이를 0.45 μm 필터로 여과하여 최종 박테리오파지 용액을 회수하였다. 이렇게 얻어진 최종 박테리오파지의 용균반을 확인한 결과를 도 2에 나타내었다.
도 2에 나타낸 바와 같이, 2-3 mm의 크기를 가지는 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 엔테로코커스 패칼리스에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.
1.2 박테리오파지의 대량 배양 및 정제
상기 실시예　1.1에서 확인한 엔테로코커스 패칼리스 특이적 박테리오파지를 대량 배양하기 위하여, 1.1에서 회수한 최종 박테리오파지 배양액을 1.0×108 PFU/mL로 희석한 후 전배양된 엔테로코커스 패칼리스 1.0×109 CFU/mL과 혼합하여 MOI (Multiplicity of infection)가 0.1이 되도록 하여 35℃에서 120 rpm으로 9시간 동안 배양하였다. 배양이 종료된 후 배양액 전량을 6,000 rpm으로 30분간 원심분리하여 상등액을 수득하였고, 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 배양액을 회수하였다. 분리된 박테리오파지를 박테리오파지 EF5(Bacteriophage EF5)로 명명하고, 2016년 9월 30일 한국미생물보존센터 (Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하고 수탁번호 KFCC11693P를 부여받았다.
실시예 2: 박테리오파지 EF5의 전체 유전자 분석
박테리오파지 EF5의 전체 유전자를 분석하기 위해, 박테리오파지 EF5 배양액에 20 mM EDTA, 50 μg/mL의 프로테나아제 K, 0.5% (w/v) SDS를 가한 후, 50℃에서 1시간 동안 정치하였다. 동량의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합한 후 실온에서 12,000 rpm으로 10분간 원심분리하여 상등액을 회수하였다. 회수된 상등액을 동일 부피의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합하여 실온에서 12,000 rpm으로 10분간 원심분리한 후 상등액을 회수하고, 상기 상등액에 3M 소듐 아세테이트를 전체 부피의 10%(v/v)가 되도록 가한 후 혼합하였다. 여기에 2배 부피의 차가운 95% 에탄올을 가하여 혼합한 뒤 -20℃에서 1시간 동안 정치하였다. 반응이 끝난 후 0℃에서 10분간 12,000 rpm으로 원심분리하여 침전물을 획득하였고, 차가운 70% 에탄올로 2회 세척한 뒤 에탄올을 완전히 제거하여 DNA 펠렛을 회수하였다. 회수한 펠렛을 최종 부피가 50 μL가 되도록 TE 완충액(Tris-EDTA, pH 8.0)에 용해시킨 후 DNA 농도를 측정하였다. 전체 유전자 분석을 위해 PacBioRSII 를 이용하여 유전자 시퀀싱을 시행하였다(마크로젠, 한국).
박테리오파지 EF5의 전체 유전자의 크기는 141,996 bp이며, GC 함량이 32%, 전체 ORFs의 수는 172개임을 확인하였다. 분석한 박테리오파지 EF5의 전체 유전자 서열을 서열번호 1로 나타내었다.
실시예 3: 박테리오파지 EF5의 형태학적 특성
박테리오파지 EF5의 형태학적 특성을 알아보기 위해, 배양액을 0.01% 젤라틴 용액에 희석하여 2.5% 글루타르알데하이드(glutaraldehyde) 용액으로 고정하였다. 이를 carbon-coated mica plate(ca. 2.5 x 2.5 mm)에 적가한 후, 10분간 적응시켜 멸균증류수로 세척하였다. 이를 구리 격자(copper grid)에 끼워 2% 우라닐 아세테이트(Uranyl acetate)에서 30-60초간 염색하고 건조시킨 후, 투과전자현미경 (Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000~400,000)으로 관찰하였다. 그 결과를 도 4에 나타내었다.
도 4에 나타낸 바와 같이, 박테리오파지 EF5는 정이십면체의 머리를 가지며 긴 꼬리가 있으며, 보다 구체적으로 박테리오파지 EF5는 머리 부분이 약 91.6-100 nm의 크기이며 꼬리의 길이는 약 420-520 nm의 길이를 나타내며, 형태로 분류하였을 때 사이포비리대(Siphoviridae)에 속함을 확인하였다.
실시예 4: 박테리오파지 EF5의 단백질 패턴 분석
박테리오파지 EF5의 단백질 패턴을 분석하기 위해, 1.0×1010 PFU/mL로 준비된 박테리오파지 EF5 40 μL를 10 μL의 5X 로딩 완충액과 혼합하고, 10분간 끓인 다음 12.5% SDS-PAGE를 수행하였다. SDS-PAGE 수행 후 단백질 전기영동 결과를 도 5에 나타내었다.
도 5에 나타낸 바와 같이, 박테리오파지 EF5는 70 kDa, 50 kDa, 36 kDa, 32 kDa, 24 kDa, 그리고 17kDa 크기의 주요 단백질을 가지는 것을 확인하였다.
실시예 5: 박테리오파지 EF5의 다양한 세균에 대한 감염성 조사
박테리오파지 EF5의 다양한 세균에 대한 감염성을 조사하였다.
우선, 박테리오파지 EF5의 엔테로코커스 패칼리스에 대한 용균 활성을 확인하기 위하여, ㈜옵티팜에서 분리하여 보유하고 있는 엔테로코커스 패칼리스 균주를 대상으로 소프트 아가 오버레이법을 수행하였다. 그 결과를 표 1에 나타내었다.
대상 세균명 대상 세균수 용균 활성이
확인된 수		 용균 활성이
확인되지 않은 수		 감수성
비율
반코마이신 내성
엔테로코커스 패칼리스		 11 균주 11 균주 0 균주 100%
상기 표 1에 나타낸 바와 같이, 본 발명의 박테리오파지 EF5는 엔테로코커스 패칼리스 11 균주 모두에 대해 감수성이 있는 것을 확인하였으며, 이를 통해 엔테로코커스 패칼리스에 대한 용균활성이 탁월함을 확인하였다.
다음으로, 박테리오파지 EF5가 다른 세균에 대해서 용균활성을 나타내는지 확인하기 위하여 ㈜옵티팜에서 분리하여 보유하고 있는 균주와 미생물 자원센터 등에서 분양받은 균주를 대상으로 소프트 아가 오버레이법을 통해서 감염성을 확인하였다. 그 결과를 표 2에 나타내었다.
Figure 112017123263133-pat00001
표 2에 나타낸 바와 같이, 본 발명의 박테리오파지 EF5는 대장균, 살모넬라 등의 다른 균주에 대해서는 용균활성이 없는 것을 확인하였다.
따라서, 본 발명의 박테리오파지 EF5는 엔테로코커스 패칼리스에 특이적으로 용균활성을 나타내는 박테리오파지임을 확인하였다.
실시예 6: 박테리오파지 EF5의 pH 안정성 조사
박테리오파지 EF5의 pH 안정성을 확인하기 위하여, 다양한 pH 범위 (pH3, 4, 5, 6, 7, 8, 9, 10)에서 박테리오파지의 수를 측정하였다. 소듐 아세테이트 완충액, 소듐 포스페이트 완충액, Tris-HCl을 이용하여 각각의 pH에 맞게 조제한 후 990 μL로 분주하여 준비하였다. 박테리오파지 EF5 배양액을 2.0×108 PFU/mL로 준비한 다음, 상기 배양액을 10 μL씩 취하여 pH에 맞게 조제한 각각의 완충액에 첨가하여 2시간 동안 상온에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의해 타이터(titer) 측정을 수행한 결과를 도 6에 나타내었다.
도 6에 나타낸 바와 같이, 본 발명의 박테리오파지 EF5는 pH3-10의 범위에서 2시간 동안 안정한 것을 확인하였다. 이를 통해 박테리오파지 EF5는 pH 안정성이 우수한 박테리오파지임을 알 수 있다.
실시예 7: 박테리오파지 EF5의 열 안정성 조사
박테리오파지 EF5의 열 안정성을 확인하기 위하여, 20℃, 30℃, 40℃, 50℃, 60℃, 및 70℃ 등 다양한 온도 범위에서 일정 시간 방치 후 박테리오파지의 수를 측정하였다. 보다 구체적으로, 박테리오파지 EF5 배양액을 2.0×107 PFU/mL가 되도록 희석한 후 배양액 5 mL을 멸균된 튜브에 분주하고 30분, 1시간 및 2시간 동안 각 온도에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의한 타이터 측정을 수행한 결과를 도 7에 나타내었다.
도 7에 나타낸 바와 같이, 본 발명의 박테리오파지 EF5는 20-70℃의 범위에서 약 30분 이상 안정한 것을 확인하였다. 이를 통해 박테리오파지 EF5는 내열성이 우수한 박테리오파지임을 알 수 있다.
상기 실험으로부터 박테리오파지 EF5는 장알균, 특히, 엔테로코커스 패칼리스(Enterococcus faecalis)에 대한 특이성이 매우 높고, 장알균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있음을 확인하였다. 또한 본 발명의 박테리오파지 EF5는 사람, 동물, 식물 등 세균외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 EF5는 장알균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
비록 본 발명이 상기에 언급된 바람직한 실시예로서 설명되었으나, 발명의 요지와 범위로부터 벗어남이 없이 다양한 수정이나 변형을 하는 것이 가능하다. 또한 첨부된 청구 범위는 본 발명의 요지에 속하는 이러한 수정이나 변형을 포함한다.
이하 본 발명의 박테리오파지 EF5를 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 본 발명을 한정하고자 함이 아니고 단지 이를 구체적으로 설명하고자 함이다.
제제예 1: 의약품의 제조
1.1 주사제의 제조
박테리오파지 EF5 1 × 106 PFU/mL
주사용 멸균 증류수 적량
pH 조절제 적량
통상의 주사제의 제조방법에 따라 1 앰플당(2 mL) 상기의 성분 함량으로 제조한다.
1.2 액제의 제조
박테리오파지 EF5 1 × 106 PFU/mL
설탕 20 g
이성화당 20 g
레몬향 적량
정제수를 가하여 전체 1,000 mL로 맞추었다. 통상의 액제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충전하고 멸균시켜 액제를 제조한다.
1.3 정제의 제조
박테리오파지 EF5 1 × 106 PFU/mL
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.
한국미생물보존센터(국내) KFCC11693P 20160930
SEQUENCE LISTING <110> Optipharm.CO.,LTD <120> Novel Enterococcus faecalis specific bacteriophage EF5 and antibacterial composition comprising the same <130> 1-36P-1 <150> 10-2016-0178158 <151> 2016-12-23 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 141996 <212> DNA <213> Bacteriophage EF5 <400> 1 tttttggtcc ccaataacta tcattataat tatttaattt tggacctgga gtagaaccaa 60 agtcaatcat ttttcctcct ccaacaacaa ttccaatatg agtggttgtc atattagtag 120 cataggttcc agcaaagaat attaagtccc ctgctctagc atctttaaca ttaacttttt 180 taccccaatt tccaaaccat tgggcatcag tataacttcc tatatcaacc ttagcatgtt 240 tttgatatag atattgacag aatgaagaac agtctccaaa accgcctttt cttatttggg 300 caggtcttgt ccaagaatag gtcattgatc caagcaaatc attggcttct ttaataatat 360 tcttaattgt gccttctcca tttggtatta aaccatcagc gccagttcct cctccgctgg 420 ttcctccact gtcatagtca ggatttgtaa ttacatcttc tcctttttta atggcatcac 480 ttaccatccc tacaaaagca gtatataaga aaggattatt ataccagcta acaggttcgt 540 ttacgccatc tacttgccaa gctggcaata cagcggttgc aggtatttta tatgctctac 600 atatttctgc aataactaaa gctaatttaa tatttaaatt agtagaaaaa ttagataatg 660 aacgttttgt tactactcca atagaaatag tttttttatt tggattactc ataccagcaa 720 ctaagctttt gcctctctct gcatgataaa ctccttctgt aaaaggcact actaattggg 780 catcattctc tgatacaata acgtgatagc ccattttttg tccaccattt ccagaattaa 840 gagtattcct tattccacct aaattagaac cactttgaat atcatgcaat acaattgctt 900 gcatggcttc taatttaaac cctggtcttg tagtaccatt tttcttgata aggttttcag 960 aaaagttttt ataaccattt cccccatcta tatctccact gaatcctccg ccatctccac 1020 cagaaaaagc atctaagtcc catgctctta aattattagc attaataact gacataactt 1080 tgcttggata actaggatct gttgcaaaac caccattcca aattgctgtg atttgggcta 1140 aagggtctgt tttcccaata gcagcacgat aattagtggc ttctccaatt aatttaccat 1200 gttccagaat agaaacatcc cagctaggat acttagcaaa ggcatcatta atagttacat 1260 atccgctacc agtccactct agggtaggaa aaacaataga ctgtccttta taacttcctt 1320 ttaccccaaa cagatttttc ccttgtgttg ctagggcact tctaccccag tttgactcca 1380 atgctgcctg agcggcaaca acagaaggta agaccttata gtttttccaa ctagcataag 1440 ctccaccttt tatttttttt aaaaaatcat ctgtaaaagc catttatata cctccttata 1500 aatactctct ttaatgtaaa aaaataggct gtgcaatgac agcctattga tttattttat 1560 ttatttttct tgtttagttt catctagacc tttttcaaag ccttttttag cagcagtatc 1620 aaattgatta gttgcaatat aaccaccgat taaaccacca attataccaa caatgtagtt 1680 aagtcctaca attccaagag caggagcaat tgccactagt actgctccga tacctcctcc 1740 tagaactact gcgattaatt tagtataatc gctaggaata aaaggtttga ttagttgaat 1800 aatataaaat acgattggtg ccaagatacc accaaatgct accaaagcag cgaaaacatc 1860 ttgaatttga ctcatatcaa tatttgccat aattttattt cctccttaat ttttgccgag 1920 attaaaagat tagaagagct agtaaaaaac ctactaacat tcctaaacca aataatatta 1980 ctcggctggc acgtcttttt ttgccatttc atctacaagc ttcataacat cgtcccgaat 2040 catagctgga atttggcttt ctttcccttt ccagccttct gggtctttga tgataagact 2100 tgcgtaaaaa attgctaaat atggatacat taataatcac ctcctttaag ttttttcaat 2160 aatcatatta gataacatat taatagcatc gaccaatgcc atttcgttta gttctatttt 2220 ttctcttaaa gattgatttt cttcttttaa ttttttaatt aaagcttctt ggtcttctac 2280 ccactcattt tttgcaaaat catatttagg attttttata ctttctggaa ttggggtcaa 2340 cacacagtct ggtggaactg gaatgccaat gggtatatca tatttttcat accagtttcc 2400 tttttcattt ttttctgata gtattttata tactgtatac atttattttt tctcctttct 2460 atacaggata attatcgttt gtaaagtata ccaatgtccc tctatacgta tctccgtttg 2520 gattgcctgc tccaatagca ggaactatga caaaaccttc attacctaca tataaaccaa 2580 cagtcttacc tcgataggat tgagagctta atgtagtagc tcctccattc gatggtgtga 2640 aacctgctgg tggtttagtt aatactttcc aaccttcatc ctttaataaa ttaaacccta 2700 cagcagccac tactaattga ccccatcgag aataatttat aaaagccgat ttaaaattgg 2760 cattacttgc aatgttttgt tcataataat ataaactaga tgtagtttgg tatccgttgt 2820 catttacctt aaataatggt cttgaatcac tatttacaaa aaaaccatta tctctcatgt 2880 acatataatc ataatcttca tgtctaggag aattaatatc ttttccagat tttataaaat 2940 gcaaaccttc tccagaatta ttcattgtat cattagagaa aaataaacca tttaatcctg 3000 atatatcact attattcatt tgaattcctg ttttaccagt atcatgatat ttttctgctt 3060 caatttctag aacaccagac aaacctcttt cttccccagt tgttggaaaa tccccaaaag 3120 agacagattt acggtttgga tcgataaaca ttaagggctt accttctgta atattatatg 3180 atctagaata tgttccggtt ttatctgtaa tgaatattgt aactgtccaa gacttagaat 3240 tatctaatgg taatgtttgt gctgtaatac ttaaaacatt tgctgctgtc gtaaaaggaa 3300 tatctacctt atctgaacca cttatttggt attgtacaga taaaatttta ttttttccat 3360 caacaggaga ccaaatagaa gtacctgaaa ctaaagtatc tgtctcaaaa ctattatttc 3420 ttcttacatt aaagttaatt acaggcatat tatatggaag taccgtaact tcgtaagaag 3480 cacttccttt taatcctcga ctatcaatag ctgttacagt tagatttcct gttgttcctg 3540 taataatacc aaagtctaaa accatatttc cttctacata attaacagtt tttttaattc 3600 ctgcaaatga tgcctcaaca ctagctggct tgccataatc tctagttgtt attattgaac 3660 tagggactgt tactcttaaa gtagaatttt tatctaaaag tattttttta ttccctgtta 3720 tagcaataga tacctgattt gtatcttcat gagaaaaacc ttcggttatt aggggagagg 3780 ctgtatttgt atcaacttct gcagaaccac tagaaccaac agtattccta tattggctcc 3840 ctgctaattt tgaatttgcc tcaatagcaa aaccgttttt tgaggcgcca gtaaatcttt 3900 gaagtatatt gttaacttcg tcttgagtta gagtccaatc tattttattg gtagttacat 3960 tactccaaga cttggtccaa cctgcacact taaatgtaat atcatattta aatttattat 4020 ttgattgaaa attatctaat tgtatagcaa gttggttgcc tgctgttaac ttaaaacttg 4080 ctaacttggg tgttactggt gctggatgag caatctgaac tgttcgttgt tgactaaggc 4140 cggacacacc atttgtccat agtttaacct tccccaaaat atttccacca ttaccagcaa 4200 attgtttaat tctttgtgca atagttgcta attgcccgtc tgtaaaatta aatttaccac 4260 ttgtagcaac attatctacg ttcgttaaat aagtccaatg actatcatct gataagctag 4320 gatttgctct attttgaacc caaaaagtta agttgtgatt aaaattagat tgtctactaa 4380 tagaaaaaga acatccatta ggaactgtaa atgttggtac cgcattcaaa gaactatgtc 4440 taggaatttt tggtaaactt gcattaaagt tcccaacaga tatcgttcca taataaactc 4500 ctgcgaatgt aacatttaca aaatagcttc cagaaacagt agtcgttttt gaaccgtcat 4560 tattatgtcc tactcgataa tctttagcta ataataattt attttgatgc gcacttaatt 4620 gagaagttgc gttctcagtt tgtttttgtc cattaattgt aacttgtgct tgacccacgg 4680 cagaagctga aatagctcca tagccgtcca tagattgtaa atataaaaaa ccggtgacaa 4740 ttgtgtagtt accagcaata tcttgtcggt ttatcgacca tctacaattt aaacgatgtc 4800 gagcaaaagc agtatatttc tctccacttg ttgccattat taaatttcct ccttttcctc 4860 ttctctcttg ggaaataaga aatggtcact cccaaataat taggaatgac catttatttt 4920 tagtcagtat cttcaccatt gtatccaaca aaggcccagc cttgaatagt tggagagtca 4980 accgatacaa tttgtactgg tttcatcata aatctttctt gcgcttctaa ttttttaact 5040 ttcgttgtat ctttatttaa agtaaatact ctttcataaa cttctccatt acgataataa 5100 cctgcaaact ctctaggagt cataacatta tatccatcgt aatcagaatt gaatactttc 5160 aaaccactac tatcaattcg aacatttgta gaatatactt cttcatgatg ggattgccaa 5220 gagagaggat aaggaccaat attaatcatt aatcctgctg cggacaatgt tgcatctgcg 5280 cttccttctc cttctagccc aattctaata gtaactgcac ttgtgttagt tactgaaaaa 5340 gtaacagaca ttcgttggta tccgatagta tcggcagagg cttctgaaac agctatttct 5400 gctccagttt ttgtatttaa tacttttaca taatatttgc tcttccctga tcgactaatt 5460 ttattagcat accagcttag agtatattct gtatttgggt taggtagaag aatatcttgg 5520 tctagcttat atcttaaacc ttttaattca aaaccagcaa ttaaaccaag ttcttctagt 5580 gattcgtcac ctaaaatttg tctaatttgg gcattggctg aatcattttt atgccagaaa 5640 tcaaacattc cagaatatag aactacacta ggtttcctaa agtcaaaata attagtagga 5700 gttgttgaat cgttatagaa tagtactttc ataaatgctg ctccagttgg tatagttata 5760 ttaatagagt aattactcca gatatcttga acaatattat ttgtttttac agtataggag 5820 tctgttccaa atactgttcc agcaaaatct gttttatctg taaatagagt aataacacct 5880 tgtccattgc caattgtttg tgaagcagcg ctaaatgatg gatcacttct agcataaaat 5940 ctaattgata gataagcatt tttagtattt ccgattgtat ttggaatacg tacttgccca 6000 gaaatattaa ttaactgtcc tggcgagaca ggaatgtctt tactatagct gtatttagtt 6060 ggtgaatttg ttccaccaat acgcataatt ttgttttctg ggaaatcact ttctgctcct 6120 ataacttgac ctatgcctgc gccattatac acccaaccat tattatttaa tccagtaaca 6180 gtagttgaga ttgttccttc aaatttagga tttgttaaca tttcatcgct aggtgctgta 6240 tcttcccatt ggaaacctgt tgagtttcta attaagttaa ccccaccagc tttatcaata 6300 ccaaaattga atttattcat ctcttgttca aattctgatt tgtttacata cgttgtatca 6360 attccagcaa aagcgtcttg catctgctta ttatattctt ctattttatc tgataattca 6420 tggttggcac tacttagctc atcaaccgca tcagttttag catatacttt cgacatatct 6480 tctgaaccaa ctacagtttg aacaatagaa tcatcagaag ttttttgagt aatttgataa 6540 atccagtcaa taattgtaga accgtcttca ttatttagcc atggaagcat ttctaaatca 6600 gaagcactta gaacaaccca tccccatgcg taagttgttt ctattttttc ttgtgcaata 6660 cctttggcac tattaacgtc ttcgctacca ttttctgtag tgattgactt ccaattataa 6720 gaactaggag tagttggatc tgacgctgta tctgttacgc aatgtccaat atacataggt 6780 ttttggctac tattataagt aacactaaaa cttgggtctg gtgtaacacc atcttcctgg 6840 tatccagttg gcgtatttgc ataagcataa tgtgcatatt cagttccatt tacactttca 6900 atggctgatt taccccaacg ataaactaag cttggattag ttttatctag agggtcacca 6960 ctaccactag aagtatctag ccataataat ccaggataag gattaagtgg agcagctggt 7020 tgtcgtaaaa ttaatggttt cgttgcatcg aagcttttat tattcacagt catatagtca 7080 aaagctgtca tagaattata ttctacttga gctttaaaac taattgaatt tgattctaag 7140 aatactactg gttcaacaac tatatatttt ttatttaagg taattccatt tccagcagta 7200 attgctgtcc attctgctga agaatctttt gacatatacc atgttacttt atttggttct 7260 actatatctc catcagattg tgtttctaca ttaatttgaa ttggagattg ttcaatgttt 7320 gttccaccat tagaataaac tttagctaca agtccgccac ttcccttgat tctactccaa 7380 gtgtaactac tataggcgct tggcgcagtt gaagtttggg taatagcaat accaatgtat 7440 ttagttttac tattaggtgt tgtagtcatt ggattaccat tggcattttc tgaataacga 7500 atccatgtat aactacttga accattagaa ccattagaac cgttcgaacc atctttgaca 7560 atattaatac tcttttgatc taattgtaca tttttagctc cagcaacata catcttaact 7620 cgtgcagata aaacgtttaa tgaagaagga gtataagtat aagttaatgc gtctgcatca 7680 tacttagtag tccatgtagt tccattagta gtttcttcta caacaatacg agatttaatg 7740 ttctcaatag atttttcacc tgtttgagcc ataccagaaa tagtaatatt ttcaggaact 7800 agtttacttg ctcctcttgg taatgacatt acttggctgc ttaataataa tgaataagac 7860 gtagaatcgt tacctttaat ctgactccag gcatattgag ttggattatg aggttgttcg 7920 ctttctcccc caacatagat tcccatatat ttatatgaat caatatttgg tgttcgtgtg 7980 tcgccttcct ctagttttac ccacttaatt gtacattgtc ctacagttgt gtttggtacc 8040 tgataaatct gcacttgtgg gctagtaggg tgtgaatcag tacttgcggt aaatgttgca 8100 gaccaaatat cagttaaccc ttctactggc ttcaaatcac caaaacccca tggacttcct 8160 gtagcttgcg taaaaaatgg tctaaaaacc tgtgttgctg gcttagttcc ttccagtgtg 8220 atggtatact tcttgccttt taccattggt ttggtgttat agattttaat aagatagtta 8280 ctattagtaa ctggttgttg tgattctggt ttaattagat tttcacccaa agccacctta 8340 ctcaaataat acggttcatc aagtaagtta ggctgatatg gtgtagatac agcaccttct 8400 tctaacttaa cttcataacc aatatcgatt gagcctctag aactatagtc cgtgctagac 8460 ccaacttgtg cataccaaga atgagatact tgtgttgatg cttgaacatt aaatgtcgct 8520 gatatattcg taaatacacc ttttggggtg tttttgggca ccattacagt aataataatt 8580 agagcattat tatctttgtc taaacatctc agtctaataa ataatctaga aatatctcca 8640 ttatatgatt cattgattct tataggtata gttaaagtat attgttttcc tacagttaat 8700 ttactaaaat catatcgaga ccatgggaac atacctacac ctacagttaa atcatttcca 8760 attttttctg ctgttgcata agtcccatga tttgtaacag tagtcatact attatttaat 8820 gattttacat tatcatcatt aattattggt gcaatattag gattccctga atagtcataa 8880 tttccaaaat cttttgaatt agaatatttt gtataggtat aagtttcttt tcctgcttta 8940 gatttagcta cacttaatat tttttcaatt ttaatatttt tataagtagc ttcaaagact 9000 gctatagcag aatctttgct catttcagtc atattatata ctccagtaga agcattaata 9060 ttaaaggttc ctcctgactt attctttaat gtataaacaa cgtttctacc agtaacatct 9120 gataatccat caaatacctt aaacgttcct gaggcctctg agaagctctc aacaatacca 9180 tttttatcag caggtaatgt tacagattca tttgttaata acccagttat tgaatcagca 9240 ccatctttac cttctctaac agaaataatt gtttgcttat caataaatga ttcaaatgta 9300 gcagtaacta ccaaagaagt ccaattttta ttccaagagg aagtagaaaa tgttcttgta 9360 tttcctgtcc cgcccaatgt tattgctgtt tgtggtcttg ctccaatata tggagtggcg 9420 ctgaaagtag caattcctgt catattttgc atattagctt gaagagttat agtgtctgat 9480 tggatagaag aatcttgatt aattttaaca gtatttgaag aagttgataa aattaatgtt 9540 tttgcagatt caccgtcaga gcccatagaa cgtgcccaag tgtagtcctt agggttgtcg 9600 ctacctttca cattgaagtc agagtatgtt ccagtgtatg acggataagc attctcatag 9660 tcttcttcgg gagtaggagt gtatggggta gcactcttca aagcaaattt agcattcttg 9720 aactccacta ttgaacctgt tgcaacacct tttccggatt tagggtatac ttgataagag 9780 aagtacaggg ttacagcatc tttcgggatt gtcaaagcat tttcaaagcg tttaaactcg 9840 gaagtaactc cctcgatttt ggatactcca gttttccaac catcaacatc aatccagctt 9900 cctgtggatt ttttatactg agctctccat gtaacgtaat ctccctcagc tagttcggct 9960 ctcaaatcta aaccaagata gtaagtctcc ccctttacca gattagtaga agccaagtta 10020 ccatcagctg tactcataaa tctataatac atttctccga ttggataatt gggttttctt 10080 tcttggtgtg ttataccgct tttgaaagtt acatcatcag tgtttgagtg tgtagaacct 10140 ttcactctaa ctgttttgga gctaataggg ttcttagtgt tctctaacag gttgacctga 10200 gggtaaactt tagtaaacct gtcagtaccg tcagcagacc aagcgtaagc tgtgtgtatg 10260 tgagaagttt ctccatctgt acctttgata aggctccaag cgtacttagt agggttaagc 10320 gagtctgctt gaacgaagtc agtatagttt cctaggtagt tataattatc gatgttagga 10380 gttcttgtat ctcctttttc aatcttgagc cagtcaattt gaacattacc atttgttaca 10440 gaattaggtt tttgccataa agctacccta cgtaaatttg acgttccagt agaattgctg 10500 gctgtaaaag tggcactcca cacatctgtt agaccttcca cagggcgtaa agttgcttgg 10560 tagttaccag cgtctccata gtataattga aattctttat cagctagctt agtccctttt 10620 aaagataccg tataagtttc gcctattgtt aattcttcta aaatatcaaa cccagcaata 10680 gggtcatttg atgttttaat tgggaactga gcattcgtta tcaaattctt acctaaagaa 10740 acagtactaa agtaccatgg ggcacctttg atgttaggtt ggtaaggtgt ggcagctgtt 10800 ccttgctcaa ctttaatatt ttcgattaaa gccgttccga caaatggctc tgttgatgtt 10860 gtattgattg tgaagtacat tctatcaaac tttgatgagt ctgttgcagc gctagaatta 10920 gctgttatag atattctagt cttctgaccc acaaccgcat ctttagctgt cagtggtaat 10980 ataattgttc cgccaggaaa tttcctatac cctaacctta aattttctag ttttcctgtg 11040 tatccctcag aaacagtaac atctgcgctt aatgtatatt gagtatttgg caataaagct 11100 ggaatatttg tgctagttcc taaaaaaaca gccccatcag ttttagtgaa agttactttt 11160 aaggcatccc tatagtcttc tatagtagcc ccagattgtg gataaaaatc actagctttc 11220 aatggaacca ttaagttagg atttccggag tagtcgtaac taccaaagtc agtgctgttg 11280 gtataagccg tgtggaagta tggagtacgt ccgtcattaa cgtttgttat agtgatttca 11340 ccacgtgaaa ctgtagccat ctaatcaccc tcttctttga tttcctcgaa ggattcgata 11400 tacgtatcca tgccgtactt agtccacaaa tattcaatag gattcctatc tgtactaaat 11460 tcaatttttg tccgttcatt atttaaaatg atataagcaa tgtagtttgt catttgcttt 11520 ctacctccac tttaaacgtt gttgaattat ttacatcagc acttccaact gacagcgatt 11580 tcccagtttt ataagcatta cttgtcccgc cgaagtttgg taccattaca ttattctgcc 11640 acttagacca tttataagta tagcctgtgc cgcctgaatc gatttcttca ccattttgat 11700 acaatcgtgc tttcaaagta gtagacccta cgccattctt caaaatgttc cctccggagg 11760 actcaatgac taccataatt gggtcgtcca tatccatcaa ggttacatac tgcttcattt 11820 tagttccaga agttcctcca acattatctg tgacgacaca caggaatgtt tgtccatttg 11880 ttactgcgtc tggatagacc gttagaaccc cctgaactgt aactgcagta tcaaatcctg 11940 aatttgcaac agctcctgat gttcctgtag ttgctgtaat ttttctccag ccaactccag 12000 cgtcagaatc ttgagatgtt acaacgcttg aatctgctgc gaaccattta tattttcgag 12060 agccattgct taagtttcca tccttataaa catcagcggt taacttcaga ctagaaggcg 12120 tattatttcg gaagtagttt ccattaggtg tactcaaaac tgctactacc gcagctttag 12180 ctagttgaac aagagtaatg tcgatagtcg cttggaaagt aattggtagc gaagtatctg 12240 ggtctgtata tactccttca actgtccaac gtacagcatt atctgttgtt ggaacgttta 12300 cttttgttgt taacacactg tttgatgtac cgctcttata ttcactatca gtatttgttg 12360 tagaagttat ttcagtagtt gttgacccta ctgttttttt ccatttaacg cctgatacag 12420 cagaaccaat taaacttcct gttccacctg cttttgttaa gttaagtgtt aaaacctgtg 12480 cagaggaccc aaaattaggt gtatatgagt tagttgtatt gttgtatgtt tgagttgttg 12540 tttgacttgc tgaaatccac gcatttaatg caggagcatc atataaatca ataattgtaa 12600 tttgaccgct tgaaattgtt gccattatta ttccatctcc tttatatctc ttcgataatg 12660 tcacagctaa atgttgcacg tttgttaaca tcatctgttt caattattat gctttttgta 12720 ccaccagcat gcattgcatt ccatattaaa tctggtgatc catcagcatt tgttttagtc 12780 catctaaact gattcgcgtc taataaatca gtaatatttt catttccttt aaaaacgatc 12840 gcatgaataa ttgttgaaat taccccattt ttaaaaatta aaccatttga tgatataagt 12900 tctacgttgt atggactctc cccatcttct ccaggagtcc cttgttttac ttttgcaatc 12960 ttagctactt ttgtcaaagt agttgtttta tacctagcaa taaaagttat ttctcctgag 13020 tctacagtca ttcctgatac tttataagtg tcctttgaag aacttaaact cccttgaatt 13080 cctgcagtta cttcaacaga aatatcccac tctgatgtat tttctcttgt tccttcaaac 13140 aaaataattg acgtactagc attagagaaa ttaccattat tcccatctgc atctgtatta 13200 acagcaataa attcatcagt taagagacca ctataagaat tttgtccatc tgaaccatct 13260 tcacccttaa ttttagtcca gatataatca cttgggttct cggaaggttt attttttgtg 13320 cttgtataaa cccccatata ttttgtatct tctgttggtc tttctgtcat aggatttcca 13380 tctgggtttg ctgaaaatct ccagaatata tattgacttt gtccgtcaat tacttttgaa 13440 ataccagcag aagctgtacc tacttgagtt aatgacatct tgttccctcc tttatctcta 13500 tatttatcta ctcattacga tatattttac atgttacata aacattatcg ttttcaggga 13560 tatctcccca ttctaatata atagattctc catacttggg ccatactgtc gttttagtga 13620 aaggttccat tggaccaatt attctagaac tattatctac caattctaaa ttccacacaa 13680 aagtgtaatt agaagtatct gttattttca tttgtcctaa ataagcttct atttgtaatg 13740 aagttggctc accatttttt aattgcgtat tgcccaagat aactcctgta attggatcag 13800 tagaatcaat taaagtagtt gttgcagctc ctttaaattc ttcaaagtaa gctaccacct 13860 tgatagaaat gctgccgtta aagccatttc cagaaataga taggatattt gtatcatatc 13920 catttaaagc tccattcaaa ttatttgtct cgctaattag agaccagcct actccaccat 13980 ctttatcata ataaggacta gaagaattaa ctgattcatc tgcataaaac cattttctat 14040 tgcttaataa agaaagaaca tctttatcag caaaatatgc ttgtagtgat accaagagaa 14100 gagattgttg tttattcttg atggttgtgc catatggatg aagaacgtta actaaaagag 14160 cattctttcc atcctctcca tcttctccat tagatacata agagaagtct aaagacaaag 14220 aatcctcaag ggttaaatta tattcttcac tataataaga tactctacaa tcaatcatca 14280 ttgtaggatt ttctgagtcc attaaatttc ttttgataaa caaagcgttg ttttgaactt 14340 caaacctacc atcattggta attaattctc cattataata ccattgatgc gttactaaag 14400 aagtaatatc atctgctcct tttagagtct tggcttgaat gacttgattt tgtatagtat 14460 aatctggtat aatattttta gtggttgggt caaacgtttg agttctactt aacttaggag 14520 ttaaacttaa tttaatattt tctatatcat ttaaatcaac tatagttatt gtatcttgtg 14580 ctaccattgc ttcttctaca tataattgta ccacaaatac ttgtttaatg tcaacatcct 14640 ctgatgtaat agggatactt ttaccagtcc tagaccaatt aggtaaaata gaaccatcat 14700 ctttcatttt ataccatcta atattttctg gctcaaatga tgaagttata gtttttttac 14760 ctctcatagc aattactttt aaaatagtat taattacacc atttttaaaa ctttcaccat 14820 ttgtagaaac aatatcagct ttataagtaa tttcatttct tagtgcctct tggattaaaa 14880 ctcttactga ggtatcttca gttaaataat tagataaaac ttgtcttact gtatttccat 14940 caacctctgt agtagtttcc atatttccta aaataacatt cattaatgat tctaaagttt 15000 tatatttaga atttaaacca gttaaatcaa tttctggata aggtgcagaa ttatttaaaa 15060 tctcttgata agacttttta atttgttccc attgaacttg taaagattgt ttttctactg 15120 gagtaacttt attatctgaa attaaatcgt taaaattgtt aataatatcc tgaatcatac 15180 caagagtaga atcggatagt ccatttgcat ttgttgttat agtcggagtc ctatcattgg 15240 ttttaccaat aataaatttg accccatcta atcttccttt aggaagcatt acatatacag 15300 aatctccagc attatatcgt ccacccatag aacttgctag aatttcaata ttttgataag 15360 aaactttata agcgtcgctt gattcaacac gttcaattac tgtaccaatc acagtatctg 15420 tggatgattg tttttctatt tcgttccttg caattaatcg aatcgcatca aaaagctctt 15480 ggcttttatc catatcatgt cagctccttt ttctcttttt tcctattgag attaaaaaga 15540 aagggcgaaa atagtcgtat ttccgcccct agtttttata ttctatttct ttccaacata 15600 agttgatgct ttgcctaata agccttcgaa cgctgcttca atttcacttc gatctctagc 15660 attagggaag tttggtgaaa gagtaatgtt ttgttcaact gtactcattc tgttactatt 15720 ttgaatatta gaaatattag aactcatatt ttgaatacta ttttttacag aagagcctct 15780 gcccttaata gattccatta gtcctctttg cattttaact gattctaaaa tattttttgt 15840 atcttcttta ttaagaatta attctttttc atgtaaaatt gccattctac cctcagcatt 15900 atttgttgaa ttactccatg ttcctgtata acctccacta tctagtgaag cagctttctt 15960 cttagagccc tcagaaactt tataagaacc atatccagct aaagtactaa ttgctgaact 16020 gaagtcaata atactatttc taccttcatt tgcatcacgt ttaacatttc taaatgctcc 16080 agcagtatta ttggttttac cagtaattct atcaatattt gacatcgcta cttttgtagg 16140 ttggtcacta aatgcgttaa ctcgttggta agcgaattta gcttcgtttc ccattgcatt 16200 atattttact gctgaattat aagttttccc acttaatata tcggtctgtt tgccagtgtt 16260 tatcattgaa ggttgtaatt gctcattcat agtacgttgc aattgaacca ttgaaccaaa 16320 cagtcctgaa tttccatttg aaccaattaa tctattttga agatttaaaa cactatttga 16380 tgttgcatca gtttgtcttt gacttcttag cattgcctga gtttgtttat caatagttga 16440 aataacttgt tgagaagctt gatttgtttg gtttaacaaa tcatttaatc ttcctgtaga 16500 gctgctataa tcatttaaaa cttttgtagc ttgagacata tactcagcat aagcatttcc 16560 taagttattt acattttgtt gagcagtaga agtccattta ttgcttgcgt ccatcattgc 16620 ttctgcaata tccatgttat ctcctaagat taattctaat aatttatcca ttgtaccact 16680 aagttcatcg gctgtcatac caatagaact tgcaaaatca tcaaagtctc cgcttaacat 16740 atcttgtact gttaaagtac catctttcat tgctgagaaa atacttgata acgtattttt 16800 atctactcct agttgatctg gtgtaatcgt accatttgca accatacctt gtagattact 16860 taatccatct tttccagcat tttctaacca tttattcatt tcattagttt tatctttaat 16920 ttcatcttct gcttcatcaa gaagtttttg taactctttc ttatattctg caggatctaa 16980 tcctttatct tgtaattctt ttattttatc ttggtattta gaaatagtat caaatacacc 17040 tttagcagat tcttttactt tatcagaatc aaaagcatat aattcatctt tttgtttctg 17100 taagtctgcg atttcacttg ataaatcttc tgtttcagtt ctaacatatt cataagtcat 17160 gttaccatta gcatcacgaa ctaattgagc tgtacgtttt gcattttctc gttcttgtaa 17220 agctaattct ttttgtttaa tatcatacat tttcattgca cgatcataat catcttgtga 17280 aactttatct tgagccaaga gagcggataa ttctttatct ctaaagtctg ctaattcttt 17340 ggcgcgttta gggtcttgtg ttttagcaat ttcgtcgtta atttctttga ttttactact 17400 gatatttgta aatttttcaa agttgctgaa atatttatct tgtttttcag taagtttatc 17460 aaaatcatca atgactccat ctaatcccca agcaccaaac attttttgga atccattctc 17520 aactgattga gtgaataatt caaaacgttt ttgagctaac tcaagttttt tcaataaagc 17580 atcgctaatt gaatcagcca tttcgttaat agcatcattt gcctcattcc aagcatctga 17640 cccttgttta acagaatttc gatatttaat cattctacgt tgagcttcct gaagattttt 17700 aacctgacca ttaatcgcct tctcttgggt atcgaaataa ccaacaattt taccataagc 17760 ttttgtacca tataactctt ctgttttagc tgctagttgt ccagcttttt caaataaatt 17820 atcatatcta tcatttacct tattaatagc ttcaactgat tgtttaacaa aatcagcttg 17880 tacttctaat aattctttat aaccacttct tgcttttgaa actgcatcga gcaagttttt 17940 attgtattct tgtaataaat cttgtctgtc agaatctgtt aaaccacggt cgtttctaac 18000 ttgattcatc atagattgcc aataagaaac attatctaaa gcttgtttca tttctttgcc 18060 gacaatttga gctttttcat caaggtctgc ataagaatct tgcatttctt taacaacatc 18120 tagaacatca gatttgaatt catctttgtc aactctaagt ttaacagtat attcaattct 18180 ttctctagca atttcagaaa gttgtttttt aatttcattt aatgttttct gtttatctgg 18240 aattaaatta aatgtaatat cttggtaacg tgaagcctct tcttgaatct ttttaacagc 18300 gtcaattgca tcttctttag cgtctccggt tttagaattt gcagcgtttt gcaaatcagt 18360 taacctttga tttagattat caatatctcc atttgctgaa aaagaaaatc cttgagaggc 18420 aagtttattt cgtaattcgt cttgctcttt atttaattca gccgtatatt gttttaatac 18480 tcctgctttt tgagctaata attgctcttc ttgcttcatg atatttaatc tatcattacc 18540 ataagcagca tctttagcat ctgataattc agaataacgt ttttcaattt cacttaagat 18600 gtgatctaat ttgtaatatc tatctaattc taattctaga ttatcaacat cttttttatc 18660 cttatctttg tcttcctttt caattttgcc atctttttca acatctttat tttctgggtc 18720 tctaggaaca ccattcagac cttctaatga ctgtggttta ttaagatcta actttttcat 18780 caagtcttca tttgagagac cgttcttatt cttgaattta ccttcaagaa tttgttgaat 18840 caaagaatct tttttctctt gttcatcttg agcttcttta ttctttttat ctacataatc 18900 ttttgctaaa gtggaaggat ctaatttttt aaagaagctt tcagaaactc caccttcgcc 18960 ccatttgtct atctcatcaa gaccaagagc gtccctcatt ccttttggaa gtttttttac 19020 catacctcca aaaaaatcac ctaatgcttt atgagctgta tttaaccatc cttttagaag 19080 ctgttccata taacttaagg cgtttcctag tccattagcc atatcgtcat aagcatttaa 19140 aaatgaagct acaagttttt gggagtaagt aagatttgaa ttagaccaga tgtcggttaa 19200 agaaatcata ttacttacat gaactgcttc cccagctaaa atttctttat tttttttatc 19260 attctctttt acttcttctc ctgcaataac ttgtaggttt tcaagactta gtgattgtag 19320 agcattttta tattcagcca acgtttggta gttagatgca tcaatgccat aatcttcagc 19380 aattttatta attgtcttag aattattttg catccatact ccataaaact gctcattatt 19440 catgaccata ccagttatag cggcttgttg cctttctgat aggaaggtat ataaatctga 19500 agcatttttt tgaaaagcct cagtaccttt ctcagcagaa actctcatag ttttttctag 19560 ttcagggaat ttttgtttta atatttctaa ttggctttcg tcaaaaccat ttttttgaag 19620 attttttaat gcttccattg cagtatttaa atcttgaaca tcacttagac tttcattaaa 19680 acctttaaca gtcctagaca ttctagcgta tttttcagaa acatcataca ttgtattttg 19740 aagttcatcg taatctttaa ttttatcatt aatattttcg atttcttttc tatcctcagt 19800 aaagtcagcg ccttctgctt tagaaaaatc aaatttcatt ttattggcgt cttcaacttt 19860 ttcttcgaga tctgaagcaa gctctccaat acgtttttgg gcttcttcaa catcttcctc 19920 tggaattatt ttatctacaa attttaatgc ttgagactgt acttcatctg taattggatc 19980 tgtggcttcc gacatttttt cttgtaaagt ttcaatattt ccttgaacat catataaagc 20040 taatttagcc cattcactag aattggcaat tttatctaag aattcttgag cttttttagg 20100 gtcttgttga gctatttcag taaactcatc tttaataaga cccatatttt gaacaagatt 20160 tttcattcct tcttcgtcat tctcagatgc cattcctgat aggaaattag agttcatata 20220 agtatcagaa aaagtttcaa atgcttcttt taattcagga gctttgtcaa ctaatttatt 20280 aaattcatcg tttgcttcat taaaaggtac tacaatacta ctttgaatat tagctttagt 20340 ttcactgaag gttttttgta cttctgaaat tttattttga gcattagtta aatcttttag 20400 aatatcatct actttttcac ctttgccctg tttttttgca ttttcaagtt ctttggttaa 20460 tttagcaact tcaacagaat aatcattcat actagattta gcattttgta agtcagaaac 20520 ttgttgtaca ataaatgaat ttctatttgc ggaaagcatt tgataattat ctgttctagc 20580 agttttttgt tgctccatca aagtttcaaa atctttagat agatctataa ttgctctacc 20640 agtatcatcg taacctttga ctaattctgg atttgtttga gcaattttac cagctaaatt 20700 atcataatct tctagatttt tattgtactt ttcaaaagct tctgtatcac ttgggtctgg 20760 tgcattatta ataaatgctt cagcattaac accctcaaaa cgtttatagg tatctgcaat 20820 atcatttaag ttagaaatat tcccattaac tgtttcttga agagacataa aagttttagt 20880 caattgttca ttaacttctt ttgctttttc tccacgagat ttgaataagt caaatttatc 20940 aactattaag gaaaatgctc cagcagcggc ccctgcagcc ataccccacg gattcataga 21000 tgccattaac attgcagaag tagtttgtaa acctgcaatt acaccatctt gtttgctaat 21060 acttttttct tgaacagaat tataggtagc tataatagga actattgtgc tataagccaa 21120 actcattcct ctaacacctt tttgaattaa ttctgttcgt tttgcaattt gagcagtact 21180 ttgagctttg tcttctaata cttttctatc agcaataatt ttattttgtt ctttcatttc 21240 attagtatat tgttcttcta atactacttt tttagctttg ctttctaatt ctttttcagc 21300 tttagtaaca ttttccctgg cattatattc ttcagccatc attgcatctc gaatttgatt 21360 ttctataact ttagcttcct tacgaatctt taaaaggctg gcatgactat catattggct 21420 atctattcct tgtcgaattt tatcaaggtc tccagtaaat tgtttagata acttttcatt 21480 ttcatataaa gcgcttcgaa tatcatcttc ccataaaact aattcttctt gcagttcttt 21540 tctaactcca gaagctttag aatcaagtaa actaactgct ccttctaaag tagaaagatt 21600 attaagtgat acttgttctg ctctttgtaa ctcttctcta gctttattta atttggtaat 21660 attttctaaa attcgagcgt ctttaccttc attttctaat ccttttgctg ctctagtaac 21720 atttttttcg tctacaccct tataataacc atttaattta atcgcttgtg cagcttcttc 21780 agcttttttc tttacctctt cagcattttt cggaatatct tttaattttt catcaagttt 21840 agtaatttct ttagttactt cttcatagtt tctcgcagcc tctggaccaa gagtagacat 21900 aatctggttc acttctgctg cagatttgta ttcttcttgt aacgcagtca aattaccttc 21960 ttgtgcaatt ttcattttct ctaaacttgc tgtaatttct ttggcttgtt tatttagtct 22020 attattggaa taaagttctc caacctgatt agcgccaaac atttgtccgc caataccaac 22080 aataggagca attgctgtag ctaaaccacc aagtccactt tttgcgtcac caataatttt 22140 taagaaatta gtagcgtctt ccaaaacacc cttaaacata tctgagtcaa caactgtatt 22200 tacagcgccc tgccaagcat ttttaagttg ttctccttta gcttttaaag agtccatata 22260 tactaattgt tgttgcgccg atgttccagc agaatcataa gcttccccaa gtaattgatt 22320 gtatcgctct tggttgttca taaacgcacg gaaagtttct gcttgatact taccagcaac 22380 agcttcagaa actaaagatt gttgttcttt attcattgtt tgccaacgag cagcaatatc 22440 tttaaagata tctgaagcat ctcttagatt cccattatca aataaagaaa tatcttcaat 22500 tccaccaagt ttaaaagctt cttgaacttt atttgtgaaa gcttctactt cttcttctcc 22560 agcttcacgt aattgggtga acgaagtaag cattgattta aatgcattac cgattgtttc 22620 tggcgtacga cgagttactt cagaaacagt agcaatcatt gttgctattt cttcaaattc 22680 aaaaccaacg tcttttgctg tattagcagt acgtgttaaa cctgtagcaa tttcagctaa 22740 gtcggaacca gatgctgctc ctaatttagc cattacatca gtaatataag taccagcttc 22800 tccacctttt tcttttaata gttggaaacc attaattgta gctgtaagat attcagacat 22860 ttgatccgtt gctacgttag aaatattagc ggctgttaca gtagcttctg ctaattcttt 22920 tgcgtaataa gctgcttgac caccttgttg ttcaaagatt aaagaagcat ctaaataatc 22980 ttttgtagtg gttcctaagg catcagcagc atctacagcg gcatcacgat atccttccat 23040 ttcttttcta gttttaccag aaacgatact aatatctgtt gaaatttcat ctaattgttt 23100 tgttacattt aaagcatcgg ttactgcgcc tgtcacagct ccaatagcac caaaagcaat 23160 agtttgtccc ataccttgtt gtaaattcat tcgtaactga tcaatttgac ctgttgttcc 23220 ttgaatttct tttgttgcta gacgagcagc gttagagata gatttaaatc ctccaccatt 23280 attagatgtt tggtttaaag atttaatttc tttctctacc tttgaaacac tagttgcagt 23340 ttgtttagct tcattttgta ccgcatttaa attagactgg aaaattctac caaaatctcc 23400 accactttgt tcagcttctc taattgagcg ctccaaagct gaaatttgta atttagcttg 23460 ttcaatttct ccatctttta agttaagttt aactccatct ttaccaatgg agtccattaa 23520 gttttttagc tcttttaggt cctgtttacc ggcatcaaga ccttcgtagg tagttttaaa 23580 ctttaaacca acgtctacat tatttgccat ttaattcctc tccttttgct ctctatgttg 23640 taaatttaac aggcatatta gctaatattt cccgttctct caactctcta agttgatttt 23700 gataagaact ctgactagca ctaggatgaa ttttaagaga taatcttttc tccataattg 23760 aataatactc tcctaagcta ataactttgt cattaaaaac catataatca atacgatcat 23820 ttcctcccat tgcaattgca tagtctgcag acaaggaagc taaaaacatt gttaattggt 23880 cacgagttcg ttttatagaa ttagaacgcc acattagtgt tccagctaac tgtgatcgga 23940 aatcttgatt attacttatt aaagcaataa gctgatttgt attagaagta tttaatgaaa 24000 ctcgtttagt tttaccattt ttagataaat tctttaaaga aaaacctaag tcaatccctt 24060 caacagttgc tctaaggtct gttttacttt gtttaacttt aaaaggtcgt ccatcactca 24120 aggttaccga aactccttct gctcctccca agagggcaat gtcagatagt gcttcattga 24180 cttggcgttc caaaccactt gctaattgtg cttcgaaggc aaaacccatt aaattactca 24240 atgacccaac cattgtcttt aaatttccca atgtttttat tgttgatggt gcttgtcctt 24300 tagctccttc ataactagga gttgtaataa ccatatttga taattgttta acagataatc 24360 cttcttgatt cattttatca atagattgtt ttaaacctac tatcaaagcg tctgcatttt 24420 gcatcgctgt catagcacct cgttctaatc taaaggcttg agaaatattt cttccacttg 24480 ccatagaaat agaaccattt atctgagctt gatccatatt tctaaaataa tataaatcat 24540 ctacccccga aagtacatca agatattctt gaactgagtt agcccaattt tctaatactt 24600 taataatttc tttttctttg ccagctaatt tttgtagtct atctgaatct ccagataata 24660 aatcattaac atatttgttt aactctgtat tatttatact gaaattattt tgtgctattt 24720 taaaattttt aataaaatct gtattttctg taactaattt actatgaaga ccagcttttt 24780 tagaaaattc ttcggcattc attcctgccc tagaagtaaa atctgcttca taagcattaa 24840 tataattttg atatattcta tgctttgtat cgcctgaatt aaaatctctt aaagagtatg 24900 attgtaaagg ctgtaaatta tagacagaat taaattttaa gtctcgtatg ttttgtaacg 24960 tataaggtaa ctttggcaga ttatcggcca tcctattccc tccttttggg ataaaaatag 25020 agacaggaga tagattaact atcgttcctg tctccttgtt ttttctcgct atgctctttt 25080 tcgtcatgaa tttcaaagtg ttctagctta cgatttattt catcaacttt atcggaaacg 25140 ttgtctgtct tttcatttcc ttctcggata agtgcgacta gttcagataa tgtttcggta 25200 aaacttgcta taattttgaa cgttttgtcc atttgttcgt tgtgtaacat agtttgttct 25260 tgttgtttat ctaaaaattt cccaactaac cagactacca aaaggatagc aactattcca 25320 atcgttaaat ctgacaaacc aggagccgca tttaaaacat ctgataaaga cattcgtact 25380 cattcctcct tgtgataaga aggtgtaatg ttagttatct gacactgttt ctgattcgtt 25440 gctgacaatt tcactcataa atttagctac catgccttca atagctttct cttgagcagt 25500 atatcctgat acagaagatt gaatagattt ttgcatttgg tctgaataag aattaatata 25560 acgaataatg tcttctaatt ctgcagggtt cattgcatct tgtactttac ccacaatatc 25620 attagattcc agtacatcaa ataaatctgc atcattctgg agcatttttt ctgtaaatga 25680 aaaattagta tattctttaa ctaattctac accaagatag gcatctagca tatcttctcg 25740 aataacgcca ccattcgcag atttcgcaat tacataagaa acaagtttaa tcttgttttc 25800 aattggaaga tattgtttta cttcaatctc ataaattgta ccatctgcac cttcaatctc 25860 tacagtgttg acactatcat caacctttaa tttaagttta ctaaaagata ttttactcat 25920 agtatatttc tctccttttt ctcgtatgtc tttatcaata ataaaaaata tgaattactt 25980 acaaaaaaaa gacctagtaa aaatactagg tcttttataa ttattagcac acatcttcgc 26040 caattggttc ttcggcaaca tccaatgcaa ataatgcttt acattttttg ttaaatggaa 26100 gataatcagg ttgtgcttca acttggaatg ggaatgtact tggatcacca gtagatgcca 26160 ttgtgaatgt gaagttagaa ctgattttac catttggaat agtaaattga gctgggaagt 26220 cacgtccatc agcttgacga cggaacaatg ttgttgcttc gattaagaag cttcctgcaa 26280 atttacctgg tgtgattgtc aagtttgaac ctggttgagc tacatagaag tcaactaagt 26340 aaactttaga ctcatctaaa gcatttgcgc ctgaaacagc tgtagttgca ctatctccag 26400 cataagcact tgctaaatag aattttttag ttacatttaa ttcagtatcg tcaccttgtt 26460 gtgactcagc caaagtagat agagctggtg tagaaggagc tgtttgtaaa gcgcctgctg 26520 taacatcaaa acgacgagta atttcaccat gttcgttaag ttccattaca taaataccag 26580 cttcagggta tttaggattt ccaccttctg ttaccaaagt agggtctgca gcacttagga 26640 tttgtccatt tttaggtaga tatggagcta aatcaagtac tggtacttca gcagcagttt 26700 tttctcccaa ttggatagat gcttttgtaa cttctacacg ttctgtaacg tgcatattta 26760 cacgagtatt ttctgttaaa ttaccacctg ataacaaagc aaaactacgt gttgataata 26820 gagcttcttc aaagttgaaa gtaactgttt tgtcaccttc ccaagcaagt aaacgagcat 26880 taccacgtcc accttgagcg taaacagtag cagttgtaga ctccaaagtt gaagttttag 26940 ctgagtcaaa gtagataact ggttcacctt ttttatactt agtgttacca acagtcatag 27000 cacttttagc cttaaatact acgtctacta tttctcgaac gccaaatttt tcattcattt 27060 gtttttcctc ctcttatatg agttgttctt ggaattatat tccctaatac atctagaaaa 27120 aaccaagctt gtattataaa gttttgatag taaaattaaa ctgactgttg ccagtttact 27180 acatcatctg cttctaaacc tccaaaagcc ccaagtgtaa tctgtgtacg atattgttgg 27240 aacatttgtg ttcgatttaa ctgaataaga acctgcggaa atgttaattt taagacctct 27300 gttattggta taccatcact cgtagctaaa attgaagcca ttgttcctat tacagactct 27360 tcgtcttttt tagccttact tttcccataa attctagctc ttctttctgc tgctgcagcc 27420 attttatcag caattttttg agccatttct ccagcaggat ttagtttcga ttcttgatct 27480 tcttctgatt gataagtaaa catatcagaa atagtttctt ttaattctga gaaattttct 27540 gcatcaagag taatagtatt tattttattt tcttctttgc taagaatatc tacaaaagat 27600 agttgaagca acatttttgt ttctcctatg ggaataaatt ttactcctcg taatgattca 27660 aaaattaaaa ataaaaagga ttcaattact cttttttctt gagttcccaa acaatattta 27720 taaaaaacat ctagctctga tgttatcgta taatttaatt caacttctgt tgctccacgt 27780 tcttcttcgg gcattccttt aagtacttgt tttttaaaat agtctggaga agaacccgca 27840 aagattgcta aggcatcaaa tagggttttt tctcctaata aagctatttc ttttattgtt 27900 ggttgatgaa ttaatgtagc aaaaggtata aaataaaagt ctgtgccagc aacttttgtt 27960 aaaatatcca ttactattgt atttccgaaa atgaaaacat catagtataa ccaagcattt 28020 tttcattaat tttcaagcta gttgctccta agaatgttgc tggccctaaa gatttcatct 28080 tagtatttga aaaaagttgg tctaattcat tcattattaa atatgggcgt tggttatatc 28140 catctaaatt ccaaacttct atgttacaca aaatatcaaa cacaaaatta tatgttaatc 28200 cttgtattgt aggcacaata tcagtaattt gtactgcaat aaaagtattt acttcaatat 28260 ctttatctat aattggtata acaccaatat tagttctcat tgcttcagct cgttcttcgt 28320 ctgtaacagg tctgtcatct gttaaaacat ctgaaccatt gcgagtaagc aatttaatta 28380 atctgtcgct atccaaaagg cgattagcaa taatatttag gttgtcgccg cttgtgataa 28440 atctatcttt tatatacata tcttttgtag cttttttggt tttactatca taaataggca 28500 tattaaaatt ctctccttta actcattatt ttatcaagaa aacatagata ctgttttagc 28560 tgttactata ttagaatttc cttcttcgtc tgtaaacatt atagtaatta tgttcccaat 28620 tttcgttcct tttacatcga cctcgtattc atctacctta ttaaaggttg cgccagaaat 28680 ttcccatgtt cctttaactt tttcagacgt ctcttcatta attgcataaa ctctgctaac 28740 taatttttct ttaatcttag caggtgcaac aatacgaatt ttattttctt taggttcttc 28800 ttcgttaggg gcaaaatcaa ttttaccatt aggaattaaa gctaccatat catctgttac 28860 cggattgttg aaatcttcaa cgcatgaaaa acgtaaaata ttaggattag aaatatcatc 28920 aattacttga attctccaac atcttccttt aatatttatt ttatcatatc ttgttaaatc 28980 attaacgtct ttaggactat tagatcccat ccatatttct acagtatcat tttgacgacc 29040 tacaatagta tttcctctag tttgttcata tttagcacga gtttcaatcg gtccttgaac 29100 tacagcccac tgttgttgaa tttgtccatg ttgatttttc catctaacca catgttcagc 29160 tttttggatt tctcctctga aaaattcatt tatattataa tcttgccatg taattaacca 29220 tctaatccca agtctttgcc aagttactgt attacctact tcaaaaccga ctccattcaa 29280 agcattaata tatttaacat cttgttgcca attatctttt tggtttttaa gtataacctt 29340 ccaaacgtca gtgtcgcctt tttcccatac tgtaaacaat tctgttcctt ctgctgaatc 29400 taacgcttgt tgaaaacttt tgtctagcat atttaacttt aaactactat ccatacgttt 29460 tcttgcatta tttaaacagt cccaagcagt atttgtatct acaggatcac taggtttaac 29520 ttgatagcca ctctctattc gatggtcatg attatctttt actttgtttg ctttgccaat 29580 atcagtgatc ttcatttatt aactacctcc tttctttttt ccagtcctcc aagagcgaga 29640 taatttcaaa tactgttttt ctatatagca taaaatcatt ttcattttcg agcccagcta 29700 atttagccat aattgttata gcaactactt cctcttgggg agataaatta attattcctc 29760 ccatttcaag taggatagtt tgtaactgtt tctcccattc gccattttct tctttaagag 29820 gtagtaattt aaatacttga tttttctttt gtttaatata tattccccat ggtacttcat 29880 taataccaaa gccattttta aattctgtat tagtcatgac gttttacaac tcctaactgt 29940 gtgataataa tatcccgagc acgtggacca actccagaaa gattatccgc attaggaaca 30000 atataaccat ctttatcaat tttacgttta gagtataaat tcaatttatg tttaatacgt 30060 ttttgttggt ctaaaataac tgcatttaat ctttgcatat gtgctgcttg tgaagtttgt 30120 ttgaaatcag atgtagaata tctcatttga ataatacgag ttgtatttaa ttgacgtctt 30180 aaccaattta ataacataat ttcagcaata atatcttttt cttcattggt taaagtatca 30240 acgaaggctc ctgttacaac tggattccct tcttcatcta aagcatcaga ggtaacaata 30300 tctggatcgt ataatccaat tctaaatctt ggataactat attctggtaa ggaatccata 30360 aaaatagatt caataatttt tagagtgtct tcaagactta attctaaata catatcatca 30420 gtgattttat ctaaaaaacg acaataaatt tcaaaaaaac ttgttccaac ttgttgattc 30480 tccatttcct tttcctccta aaaaaaatga ctggctcgta agccagccat tctattttat 30540 cttattttta tgttctgcgc ttacgccctg agcttgctgt tccagttttt gttttacctt 30600 ctgctcgttt tccagttgaa cgacgtcctg cgcttcttgc agcagtatta gttttaacac 30660 ctttaattgt ttggtattgt agtcgtccag catcttcgtc cccagcagcc catttaacgt 30720 tctcttcaag agcatcaata ttaatttgta gaacttgtcc aataaaatga cgacgtttag 30780 aactatctac attaatagct tcttctttaa tccattctgc aatataataa ggaccaaact 30840 ctagcatgtc taagatttca tcttctgtac ctttttgtac aatgttagct acagtttctc 30900 ggtcatattc tacttctgga gtatcttctg ctaatggtaa tcctaaagct tgacgagcgt 30960 cattgctatc aatttttagg ttgtcccaaa ttaattgcat gcctccaggc atattactta 31020 aagcataaag ttctccaaat ggaatatttt tagtatctgg tcgctgacct tcttgaaatt 31080 gacgaaaatc tcttgttact ccaatttctt caatgatata agcaactctt tttcgcccca 31140 tatttgagac aggaatcatt gtatctcctt ttaacccctc gacaagataa ctaaaatcaa 31200 tagttaggta tgaattattc atatttattt ctctcctttt tctctgttct aatctattta 31260 gaactaacta aagtatactt aaacaaatat gccccaaaag agcaaaagaa aaggcccgag 31320 tcttaaactc gaacctcttc ggatttttat attaaaccaa acgtgtatat gttggtgtaa 31380 cattgccttc tgtttcttta ataattggtc cgttaactac aggccattca ttaagggcag 31440 tgttttcata gatacagata gctgggttag ccatcaacat agtacccatt tttttgtaga 31500 agtgcatttc tttagaccag tcgtctaaat ctacttcttt agtttgcatt ccgccttcaa 31560 aagcaatctt aactggtttg ttgtctacat ctggaaggat ccaagccata cctaatggta 31620 atgttactgc ttctgcttcg tttgtttcgt tgtagaagct gtgtggtagt acaacaatgt 31680 tagcaccttt atagtttccg atgaaaccat tacggttata ttcttctttc attttttctg 31740 ttgctaaatg tccttcaggg atcatttcag cagcaaatac atatgaagta aagattgttg 31800 gtgttgcata agcacttgaa ataccaagta atgctgcaaa acgacgtggg ctccaaccag 31860 ctacactata gatgttgtta gcaggtaaaa ttgtgttaga gtttaacgct accatatggc 31920 gtagaatttc tttataaaga atttcttcgt agccttcagt aatgcattgg attaattctg 31980 caaagtcaat acgaccttct aagaattctt ctaagtccaa tcctacagca gcaccataag 32040 ctgttgttgg catatcgaat acttctctgt ctagacggaa tacttcgtat agaccagcat 32100 gagcaacttg tgtcacaaag ttacgtccac ggatgttacc tttacgacgt ttaaatgtta 32160 ctttatctcc atctgcaaca tttttaattt ctgcgaaacg attcattgaa tcaaatactc 32220 gtttaggtag taaatcatca acgacttctg ataataaagc ataaactaac tcacggttag 32280 ctgtgaaacg atgtccacca tttcccatta aactattcat ttcttcacga agagcttcat 32340 tcaatgtatg agaatcatat gatttatctg agaattcaat agattctcca gtttgaatgt 32400 ctgctgcttg gaacattaaa gttctaattt gttcaggttt taaattcatt tttccatttc 32460 ctcctatcta attacgcgcc tgtagctgct ggatttactt caacgatagc aagttttgct 32520 gcaacttgtc catcagccat tgttgttttt tcaactactt ttgcaataac aacgtttaaa 32580 gttcctgctg tagataaaat acctttatca tttaatttta atgttgttcc tacttctgga 32640 agttcaccat cagaagtttc gattaaatta gtagtaaaga tatcaccagg cgttgggaaa 32700 atcaaacgag ggataactgt agcttttgga gcttcttttt tgtcataaat tggtgcttga 32760 cgcatttggt taatagaagg gtttactgta aataaagcaa aatctttatt tgaagtatat 32820 tcactatata atttaacctc actatatacc aaaccaacat ttttactttg tgctgttggc 32880 aattctaatt gaccagtttt taaatttcct tcaaaacctg tgccataaac tgcatctaag 32940 aacataccat tttcaatacg atctccgcta gatttaacaa cctctggttt aacaggtagg 33000 tctgctaaaa ctcgaccagc tcggatacct tgaatacggt ttttctcaac ttgcccgtag 33060 ccgacttttt ttaataattg ttgtgccatt ttctatttcc tccttatttt tgaaatattg 33120 cgtggtttac tcattttcaa atgaaaataa gtaaaccttg attgcaatat tttgatagaa 33180 ataatttcct attttaaatt aaaaacgtga acgtttattt ttcttagaaa ctttttctaa 33240 gaaaagcgct aaactatctt cttcatctaa cgcatctagt tcagtttctt ctgattcaag 33300 tgaaaattga ataccttcac cagactcttt ttgaccacca tgagcttctt taaattctcg 33360 gtgttgtgct aatgctgctt gagcttcaac ctcttctaaa gttaactcat caaatttagc 33420 acgaatttta tcttttcctt catcggaaag actaaattct aataataagc tttcttttgc 33480 ttcacgttca gcttttagtt taaattgtag caatgcttcg ttttgttgac gaagattttc 33540 gttttcaact gttaattgtt gattttctgc aataatagct ttaaaatcta aactataatc 33600 taattttttc tttttctttt cttcttctga ttcttcaact acagttttat cttctggtgc 33660 agattcctca gatttttctt ctgcctgttc tggtgctgga gaagtttccc caccttcgtc 33720 agttgctcca ccatcggtaa cttcaatagt tttatcggct ttaacaactt ctttaccatc 33780 ttcttgaaga gtttcttcat cagtcatatc ttcaccttca gcagctagtt ctgaatcagg 33840 tttgtcagat ttaacatcaa tttctggttc tgtagcatct gcaggagtac ttactgtttc 33900 agtacctaaa tcttcttcag ttgtttctcc ttcgactgtt acatctccac cttctggtaa 33960 accttctggt acagctgttc ccaacatttc ttgtagttta gtacgcattt catctgcggc 34020 agctacacgt tcaataagat aatcaaataa ttcattatcg ccaacatcag ataataaagc 34080 gctcgtacgt ttatctttag cggtttcacg ttcttctgca attgtagcag cggttgctcc 34140 ttgagggtct tcttgtactt cctctccagg ctgtcctgct tcttcctcaa ctgtatctgt 34200 ttgaactccc gttgcttctg gttcaactgc tgtcttctct ggtttagcaa ctgtctgctc 34260 tgctccaggc tgtcctccag gttgaacttc ttcactgggt tggtcttctt cttgactcac 34320 aggttctttt gtttcttcta cctgtttagt ttcagcagtt ttgtcttcgg gattaacttc 34380 agtttctact tttgtttcct tttctacagg ttcttgagta actggatctt ttttgatttc 34440 ctcttctttt ttctttttga tatccaattc cttttcctcc tctttttcgt cttcattttt 34500 tttcaataaa gaattacttg cttttttata attgaggttt tctacactaa cgataccatc 34560 tttatagcct ggttgacctt gtaatccacg acgagctgcc tctgttacag ggattatatc 34620 agcttcacgt ttcatatcaa tttctgcgtc aactaaacct gaaatagcat catcaattct 34680 atttctagcc tcttcatcct caactaaatc agccgcttca tctaataaat caacagcttc 34740 agtaatcttt tcacgattaa ccttgtctct ctcttctcca tctgtatcaa ctacaagagg 34800 agtaccaaga gcagaatcat caaagttgtt atctagggca aattttaatg catccataag 34860 ttcatctact ccttcttcta ccttatcttc ctgttctgtc aatggttgat aagagaaatt 34920 aactccttca atagaggcat cagcgaaagc aggcgtaaca tcatttccaa gtatacataa 34980 accaacaaaa gcagtcttag taatttccat aattctgccc ttttggtgca taattgtatt 35040 ttcaaaactc aattccattg attgaggacg cccatctaat gcatcaatta attctgcata 35100 tcgacttgtc catagatagc cataggttac taaataattg tcttcgtccc aataaataat 35160 aggattttct ggaattacac ctactgcttg tgtatcggct gtagttacat attctcctaa 35220 ttgattatat actgcttggg ttccatgctc accaaaatct cctttgtatt gattgtaata 35280 agcaacaatt ggtgtcagac ctaagcttgt ttttgcagca tcttctaata ctgctttagg 35340 aatatcatat ccatttctat taatccctga ctgtgcaatc ttaattttaa ccttagaaag 35400 cattggattt tcttcaattg cgacaggttc taagaattga atatctgtaa aaaataatag 35460 tctttcatct ttcaattttt atttcctcct tatcctccag cattttgaat tgttttctca 35520 gatttttgtg aatctggcaa tggtggtcta ccaccgctgc ttgaacctga agaattagat 35580 tttcctgatg ctgtgtgtga tgattgtaat ggtttcataa tacttcctaa gtttaaaata 35640 gaattttcaa agataggtga agccataata tctagttgtg attgacctag tgaaagagct 35700 ggtaataatt ttgaatatcc taaagttgcc atatctttat attttgtaga taattcatta 35760 cgattattaa ttgtaattgg tgggaaccaa agtgatgtat caaacatatc atcatcatct 35820 tctaagaact cattatctaa taggaaattt aaccaatttt gatatttttc aacaagagta 35880 aacatcatag cttcatcaat attcacagat ttttctaaag ccatattacc ctctgtagag 35940 aataattgtg gtgaaacacc aagttctgca tagatagatt tagtccaagg ctcaaaatca 36000 atcggatcag cagcagcctc atttaagtta acattttgga tttctccaag agtagtaagt 36060 aagtctgttt ggttactgtt ttcaaagatt tttttggcac tttcgtggaa agcttgcatt 36120 tcaggtaatt ctaagatagg ttctccatca tccaagaaac caaatttttg aactactaag 36180 ttatatagtt cattttcagc ttttttcttt tcaattcctt ttagttccat tacatctaaa 36240 atagcaaaaa aactattagc tagtacaggt tgtaatgaag ggctaaaata aaaagctgta 36300 caagtatctg ggtttaataa gatccatgta ccagaattgg ttaaactttg attttgacgt 36360 ctcttagaac tgtttcttcc tcgatgatta tcatttttcc aaatattata ttcacgtaca 36420 atttctggtg ggaagttatt taatttaata gctttttctt cttctgttcc aaattgttca 36480 aaataatcta aattaaattc tactgtaggg aatccattta gaacacttct agttctgcaa 36540 taggatgttg gcaatttttg aaaaactgct tgctttcctc tacgtctaaa atatacataa 36600 gctactccat ctacaataac atctaaggaa attgattgaa gagattgctt taattttgct 36660 ttttctaaga acaatagttg attaacaact tctcgtacaa tagtatcttc attaattttt 36720 gtgcctacta cacgaggagt aatcatataa tcataagttg gaaggtaagc taaatattca 36780 gccgcacgag aatacacacc agaaactcta taaaaatgtc ttgacaaagt tttaatgcct 36840 ttaacatctt ttcgtctaaa agctttatcc aaagctttac gaatgtcgtc agttgttaca 36900 aatgattctc ctaattctga agtataagac ccgaagccaa aattagtgaa gcttctgtca 36960 gcataataag aaaaatctac tgaaggtttt ttattttcgt aaagttttct ttcttctttt 37020 ttgactggag ttggctttct tctctgtctt tttgatttgt ccaacatgaa ttatattcct 37080 ccttttctct attttatctc ttggaaaatg aaatctttcc ttataaaact atatattaaa 37140 gaccgatatt atgcaatatc ggcctctagt ttttatatac ctgctttttc caaaagatat 37200 ccaatatcaa caatttcaag ttcccaatat ggaatttcta ctaatgggat accatgatta 37260 tgacaatata accgtttttg atggtcgttg tattgttgac gtttaaattt attttttcct 37320 ccaaaagcag caactggttc ataatgttgc tcaccattga cttctatagc aaaatctata 37380 tttccatcgt cgtccattac cataaagtca aaagctaatt ggcggccgct agaagcgact 37440 aaatcaggaa agctgtattc ttcttcccaa ttaaaaccat tttcttctaa gatatgacga 37500 attcttagat gtcctctact tactccagaa aatgtactca ttgttccacc tcttttcgtt 37560 agttaaagaa tgcaaagtta gcaagactac ttttcttctt attccgttct ttgtcctctt 37620 gtaacttaat tacataaatt gcatacatta aggatgaaac cttatcttta cccattccct 37680 tgttaattct atctaagcta aatttagtgc tattattttc aggtctagtt aaattcatca 37740 tttcattctt tagtgtagac gttaatacaa aaggtctaat agcgtcagct ttttgtcctg 37800 ctgacattgt tttatatgct tttttacttt ctaaatcacc ttttgcatca cgctcgtttt 37860 gtaaatatcg aattttacca gaacccattt gttgaagtag tgcagtataa ccctcaaagt 37920 ttaattctga gtcggcttta acaatccata aaactttacc atagctatcg ccatgattat 37980 caattttttt ataaagacct ttatcatcgt taacaacctc aaaagcagaa aaactttctc 38040 ctgttttact atctgaagaa ggcataacta aataatcgac caacccaatc cctaaaccat 38100 taccatcgac tacaacataa cgaggcatat aatttaaata ttgacgtttg atttcgttag 38160 cttggattcc gaaatgctcc gattcaataa caatcatatt aactacagat ttaattccta 38220 caccattacg ctgaggattt accttaacca ctgtaataac agtttgccaa ccattacgac 38280 ctacatcgac tccgagtaca taataagctt catcatttcc tcgaccactt cttgtaaatt 38340 cagcctgttt taaaactcta catctatcga aaacgtctcc attatagaat gattctgtcg 38400 cagaaccagc ccaaacactc tcatctaata tacatccaaa ctttcggttg gaactagact 38460 atatctttaa atatatctcc aaatgaaacc tttgtgattt cctttttttc cttttaaaac 38520 atctttaatg ctatgacgag aaacatgttc ttgtcttgat gcttcaatta tagaaggata 38580 aatacaaatt aaattttggt tacaatcata tttagctatt ttattagaat ttaatttttt 38640 agttttttct ctcgtaactt cagaaaaagg tggaggagta aaacctaatt ccttttttgc 38700 atgagcaata ttttctttcg gagaaaccca ctctaaattc tcaactttat tgttaagttt 38760 atttccatca atatgattaa cctgttcttt gtgatgcgga ttaggaataa agttaatagc 38820 tactaaccta tgaatataaa cccgtttttt tccattaaga gttcttatat tcactgaacg 38880 atagccattt ttccctattt gtccaactaa cattttattt gtatttttat ttttaatatt 38940 tccatctgga tatataatat aatttggagc aatatctatt atttttccat tcatagtaat 39000 tcacctctta ctatgattat atcatattta atatatttat gcaatatttc ggtttcccta 39060 ctccttacgg atagtcgttg aacgttcccc atgtgaaagg ggcttcgctg ctgattgtcc 39120 atttatcttt ttaattttta acattcacac ttagaatttc ttcttatgtt gtagtttaaa 39180 aagctttagg atatcccagc aattaattgc attttacgtg agcatgagag attcactcac 39240 ggttaaatga tacctcgtca aaagtaccat cagactttag atcgttgaca aaacctggat 39300 ctaataatcc cattttaaca ggagttctcc aagttccccc cataataata gcggtaccag 39360 gtttaattaa ttgccatact aatagctgaa tttgtttttg gtaactaaaa tgatctttaa 39420 actttatctt cccgcaaagt cgttaatttt gcagcgttct cttatgaact gctatatgtt 39480 accatataga agagactata tcttctccct tttcaggagt cacccgtttc cattcacttg 39540 aatgtacttc ctttcggaat agtcgttgaa cgttcctatt atatttttaa taggcttcgc 39600 tgctgattgt ccttttataa aggatttccc agcaattaga gtgagtgttt atagtgagag 39660 cttgtatgtc aacccgcact ggtaatataa acttgagatt tattcatttt atcatttggg 39720 tcaaccttcc cattcgccgc acggcgtgat acgttcatca tcaattattg ataataattg 39780 gactatttct tcatcctctc ttgttattta aattaggatg ggtggcgctt cagatggtaa 39840 catctccatc ttactccttt cggatagtct ctacaccttc ctttatgatc ttcacaaagg 39900 cttggcacga gattatctta gaattgtatc aagattctaa ggtttccctc gttagccctt 39960 tcggacaccc tcttgtaatc gaggttcacc accttatctc tttagtatta ctactaagag 40020 gcccagatat gtttaggcaa tataacttgt gagaattttt ctccgtcaac caaaataacc 40080 tcgtcaataa gtcctgaatg tcgacgtcca ccacgagaac tttctttagc tgctactacg 40140 tcgaatcgac ttccattttt aaatacaacc tgtacataat ctttaccatt aagcttcgtt 40200 tgccctggcg cccaattgat ttctttttta attgatggta tcatatcaca aatttcatta 40260 attttttctg cagcaatacc agcagcctgt tctttaccac cagaagtaat aaatacttta 40320 attcctggat ataaaataca tttttccatc atcagcataa cagataaaaa tgatttagaa 40380 aaggcacgtg taaaagtacc gaaaaagtat ttatgcctca tagcagcacg taaaaataat 40440 ctttgataaa agaaaaaatt aaatttgcta tctggaccct ttatcatatc aataaacata 40500 tctggatatt ctctccaaaa ggagacatat tctgtaaaag taggaatgct tgcgcgcact 40560 ctctcttctg ttaaaaatac ttctcgattt tttttagagt tgataaaatc tgatttaata 40620 tcaataatag ttgtactttt atcttcatgt tttttattct tcgccatcaa gctctacccc 40680 caataactta gcctcatccg taagttctgg tagaacctca gtattaacag cttcaaacaa 40740 taaatcatca tcagaaatca tgtcttcttc cacaaataga gcatcatctg cagacatttg 40800 ttctttttct tcgtccaatt taatttgttc taagcctttt tcaattagag aatctaagtt 40860 taattctttt tcaactaaat tacgtacgta gtttgtgaaa tctcgcaatg taacgtccac 40920 gatatcttgt ggctcatctg tatggtatac aggaataaat ccttcttctt cacataaacg 40980 caccatttca gagatactat caatgaattt atcttcttcc ttctcttggg aagcagtgaa 41040 tttagcagat ttcatcattt tatcataggt attagataat ttttggtatt catcatacat 41100 tcctgaatca attaaactgt gcatacgtag agaaacttta cagatttgtt ttaaataatc 41160 ttcatgggaa gcagaagtga tatcataaga ttcgtgcatt tccccataaa aagtttctaa 41220 ttgaattaat tcttcttcgt tatataaagt tccccattta atttttaatt cttttatttg 41280 agttttagtt aatgcgggat cttcatcttc tgtatcaatt ctccctgcca ttagttgcat 41340 agcagtatca ggatcataac ctttgtctaa taaatcttct agtttttgat gaagtatatc 41400 acgtttttgc tgaatttctt gatcgtgttg ttccataaat tgaggagtgt cagagaattt 41460 ataaccctta tattgttcta atcgcatttt accaatataa cgtcctaaga cagattggtt 41520 tgcatttttg ttacgaatag ttttaccatc tttagatgtt gagaattcgt atcgttctct 41580 taatgaattc cattcatctg gaatatatgg aatatcaatt tcttccataa tcttcattac 41640 tgttgatgga gattttaaat caataagaga tgccatacat ttcttacatt ttttcattgg 41700 gtctccattt ttatctttat aaaaatcctt tgcaggataa aatctattac aatgttcgca 41760 gtgaattttt tctgcttgca tttctactac tgacttttgt ttttcttcct gagccatttt 41820 taaacctcct taatttaata aaaatgtcca gcttcagaat aattgaaagc tggacataat 41880 gtcttatatt attttgtcat tcgttctgct aggtctgcaa cacgcgagcg ataaatatta 41940 cttaaatgaa tattgccaaa aacttcggtt ccggcaaaaa cttcaattaa tttaagcatt 42000 ccattattat caccttcata agcccacgaa tccaattgag ttgttggatc tccctcaata 42060 aaaactttgg ttccttctcc agcacgttgt aaggctaatt gcattaaatc tttatttaaa 42120 ttttgagctt ctgtaatata taaagaagaa ttttctgtaa cttcataacc tcgaatatct 42180 gaaataggaa taatttcaat aagtccagat tcaatatatt caattgcttt ttctcgttca 42240 ccaatttttg atgctaaaat tcctccaata gattcttgca tcaattttaa atttctatca 42300 cccttataaa aaccaagtgt tttagctcca cgagcctttt ctggattgac aaatatagta 42360 attttgcaaa tctcttcttt ttctagcatt tcaaattgtt ttgcaagcat taaataagtt 42420 tttcctgacc ccgcaggacc agttaatagc gaaatatcat gcttgcctaa catatcaaaa 42480 gcacaaaact gagcataatc tttttcttta ggatttaaag tgccaaattc agaagagcat 42540 attttaatat gtttattgta tcttactgga ttattatcaa agatttttgt ggcacgacct 42600 tcttctacaa caaagaagta aggttctcca tgtttttcta atactaagta ttcattttct 42660 aatagcccaa gagaggaaat atattggtta gctagggcaa atagcattgc gtttgtatca 42720 tcttctaatt cgtcggagac atcaacaact gcatatcctt ctcgatattt gtctgcgtct 42780 tcatagaagg ttcgtacatt aaccctattt gctttagcta ctgttcccat ggataggtcg 42840 ttagttatta cttcgatctc ggaaagcaaa cgattctctt tttgcgccaa gaatatgata 42900 tccccatcta cagtagattt atcataagag ggtctttctg gattttctgt gtcgataatt 42960 gtgaataggt cttcattttc tcggagcatt cttaagccat ttctagcttc aaatcctcgc 43020 tgaccttcag cagtttttaa tctatctagc tctcctagag ttccaagtgt aactaataaa 43080 tcacaatctt taaattcctc aattaaactg ttaattcttt ttgtactcat caaaatattt 43140 gtatccaaaa ttactcgttt cttatctgtc atattagtaa ctcctcgtta ttttttgctg 43200 ttacgacatt ctttacaaat tgaataaaac tgtgatgaag atgttctatt aatactaaaa 43260 ttacgattat ttgctaattt aatttcttta caacgagaac aacgtttgta agttccctta 43320 agtttgttca tgtacatata atcaatccac tcttctgttg cagcgtctgc aataatttgt 43380 gggattttat ttctatataa agatgaaata tattcttgtg tatgatctac tccaaaaatt 43440 tcaataagtc cttctcttat ttcatcattg cgtgacccaa taattttttc atttaaaata 43500 taaagtaaag taggtttttc agcaagagat ttttgaatta atctatcaag atcttctaaa 43560 acccatttaa tatctgaatc attattatct ttaaattcaa tttttaagct actatagttg 43620 attaataatg ataatacttg atctcgttgt tgtaggttaa tagtatcgta atgaatatct 43680 tgttcaatat ttcctgtatt acctcttcca aaaattggtt gacgatgaga atttttaaga 43740 acatattggt ctctacgcat ttcaataatt gtttgtttaa ttttccctgt taatttttta 43800 ggcatatctg gattttcatc taaatatttt tgtaatttat caatttcttc tcttagttgt 43860 gccaaaccag gtacttcttc aatatctttt tctgttattt ctacctttgg tgttaaaatt 43920 acatttttat cttgtttaat taattgatgg aaagcacttt ctcctccttc aagcttttcc 43980 attaatcctt ctcttgaagt ttctctttta tttactgtaa ctaaacgatt agctgtaata 44040 atttctccac gagattctgg attagttcca tctattaaat atgtagcaat tttatctcgt 44100 tgatatggag ttaatttatc ttctccaatt cttttcatct ctttattaac ccattctaat 44160 ctttcttcat aggtttgtaa agcattattt aattgtaatg atgccatcta tagttattcc 44220 tccaatattt tatgtctttc tatatttata ttatatcata tagactaaat aatgtcaagt 44280 aaaaaaagag aactttttca agttctcttt ttaatcttaa ctatttctta aattttccaa 44340 ttcttctttt aatgaatcta cttcttgaga cagttgttgt gtcgctctcc ataaataagc 44400 tgtcagcgca taagcgttca cgcctcgccc ttccatttct actacctctt ggggaagacc 44460 atgctcaata ataaatccta attgagtact ttgttttgga tcatttttat aagcatacaa 44520 ttcaggtgta acttctttaa cttttgataa agcatcttct tcaaatttct taataggatt 44580 cttatatttt acagaagaac gtgtaataaa accatcagaa gataaacgta caaaattatt 44640 acgttcattg cttgtaaagt ttgcattacc cttgaatgag tcaacaacta aagtttgact 44700 atattgctca taatataata cagcatagtt tctcaaagca tttctctcac cattaagccc 44760 agcttttctt gggaagatac gtaaacatac atccccatca ccagtaccct tagaagtttc 44820 atggttaaaa ccaacattaa tagctgaccc agaatgaatt ttacctttag ctcttgtatt 44880 ccatgcttca aaatacaatg tagccaattc agaattactt cccataccag caatacgtaa 44940 atttttacga ccaccgctac tgttaccaga aagcaaacta ttcattccac cattaacctt 45000 ataatgtagc tgtccatttt tacccttaac aaataatgga acgttactaa aaggcgcaac 45060 gatatcaact gaagtcccac cattattatt attattcaaa atagtagttc gtccattagt 45120 acttacatta ccaaataccg cacgaggacc atgtgcatga atatcaaccg acccatcgct 45180 ataagctgac atgatatcga cattcttcct cacattagca attctaaaac gagaaccgcc 45240 ttcttttcct ccataaggag aattcttgga cgttcctctt gggtcatcca atattgccac 45300 attattagca gaacctgtac tactaaattg tacttgagaa acctgcccag aaccagcttt 45360 cattactaga gaaccagtca tagtatctcc agctttttta acaaaattag ctccagctcc 45420 ggcggcatca attttatcta aaatatcttg tgctaatgaa atagtttgtc catttgccaa 45480 tgtctttcca tcagaagcta atccctttcc tacatttacc tttaattccc caagagagga 45540 atcaacagta ttttgtagtt tggcaaggtc cgattgggcc gctagtgggg tgaacctatc 45600 gttgaccctt gtttgggcgg tgccatcatc gccaatgacg aatccataag tattgttatt 45660 agaagccttg cgttggatga gccaacctgt tttgccattg gggcgatttg cggcagtagt 45720 tgtaatataa ttttcagtgg attttaatag agtattaacg tcaccattaa aatcttttaa 45780 ttgcattctg ccaaaatacc atgggaattt ttcggcgatt tcagcaagag cgccttcaac 45840 atctaccgag gtataattat cggcggtatc attgattaaa acattaccag cgatgttgct 45900 gcctccacct gtccctggaa tactgccggt gccaatttca acccattttt cggcggtgga 45960 aatataattc tttttatttg atgtattata ataaaataga cctggctcat ttggtactcc 46020 cacaggaaca gcatcgccaa tcattttccc tggggaaatt aatcgtgggt taccttgatt 46080 atcagcaaca taaataccat ttggataaga aaaccaaata ttttcatcgc cagattctac 46140 tttagcgggt ttgccgctaa ccttgtgaaa cttaatcatt tattcacatc ctcctttacc 46200 aatctaatgt tgtgaatact ggaactcgag agctaaattt aatattatga ttaataacgt 46260 caatatcaat tgtgccatcg gtaataaatt ttaaaatccg ctcggcgtta tcagctgtaa 46320 tttctacttc tccattagtt acgacgttaa agcccgaacc atgcagaagc tcttcttcta 46380 tgacgtgttt tgccatgtcg aactcctcct ttatttacat ttactctggt atcaatataa 46440 aaactgggca gaactataga taaatctacc ccaaccgtga gacgccgtag aaaaaagaca 46500 gtcatgcgat tttcaatttt gaagctctga ttaagtgttg ttgccgcggc ttccctgatt 46560 gacttgtgaa tattttcaca aatcccaaaa ccatccccac caaaagctat ttcagaaaaa 46620 ataacagcct gtaagacccg catgaaccca acccccatgg gcggaaataa caattgtaaa 46680 tccaattcca atctcaattc cattcctgaa aacgcccaaa ccctaatgac aacaagcctt 46740 ttaccactat tgtaaaaact tattgaccta gttaaataac gacctgtata ccccttgcca 46800 cctactccct tgtttattaa ataacattca tctgcgttgc tgccaccaaa aataacacct 46860 gttatttaat aatatcaact aatttctaac gttgagtgga atccttgtga aatatttcac 46920 aatcgtttat ggttgtgatt ggtctagtcc atctgtacct actgccacaa tatatgaata 46980 tatgttcata tgtatatcta ttcaagtatt taagtatgtt taaataacac ttgttatttt 47040 aaacgccgaa ccataaaatt aaataacctt tgttattcat caagttgctg ggaacgaacc 47100 gaacatatgt gcgccaagta tctttgcttg acaccttgcg tcagtgagtt gtaagcgctt 47160 acatctgtga caatgctcac agactagcag tgagtacata tgaataaatg ttcatctgtt 47220 catctataca tatgaataaa ttattatatg aatgattgta catgtgtgaa atgtttcaca 47280 atgtattagt tcatcacatc tctgcaccaa acataacact tgttataata actgataaat 47340 gttattttat ataagactat ttatttcagc accttaataa aagacaataa tagcgcccac 47400 aatacacttc tttgctcttc tcttgggttt tataatgctt aatagataat tatgccaaat 47460 aatatgaaaa gcccttaaaa cgcaaaataa agcgctttca attcatctct ttaagtaaat 47520 cttttgtaag cgcttacatc tatcgatagt ctattaaaat aacatctgtt atcaaatgca 47580 tatatacata cataatgtat ataaaaatat tttgtctaat gttaaaaatc atttgacaat 47640 tagggtttta tatattatac tatttatagg ggttagatga atagccctaa aacttactgg 47700 aaagaggtgt ttaaaaatga ataatgaatc tcttgaagct attgagattt tagaaattgc 47760 tttagagtat gacctagaac gaaagtacct atctaaatat aagaaaaagc ttaatagccc 47820 tttagttgat agctatgtac ttagtgaatg gttacggaac aaatatgatt ttagtatgca 47880 atttcaaaat agatttgaca ttcatcaagt ctataatgat tttgttaaat ggttaaatga 47940 aaatagttgt tgacaccacc ccgaaactat ggtatactaa ttatagttaa aagaaaggaa 48000 tgatttttaa atgagaataa tttttggtag tcttatgtta attgttatat taggtatgat 48060 gttgtttata attggttaca gtattagtga agcgtggtac agaagaagaa tatacaatca 48120 tgatccaaaa ttaatggata gaaaagaaac agtgacaaca ataaataatc gagttactaa 48180 agtagtaatg tttcgatcaa atcaagaagc ttccgaaaaa attgtaagga atgctggcgc 48240 tagtggctca tttgcatgga ataatgtttc aattttagat atatatttag tagattttga 48300 tactaacgaa caggtatgtt ttccagtagt tggtggaaga ataaacaatt ataactatcc 48360 attgagagat agtgaaattt tagctcttga attatctaaa aatttcaatg aagaaaactt 48420 aaataaattt ttagataatg aagaagtaaa agaaaaaatt gacgtattag acagaaatat 48480 aatcggaaaa gaattagtat aaaggacttg caaaagtcct ttttttgtgc tataataata 48540 ttgtaccaag agaggaaaga aatgtaagcg ctttcattga aaaaatattt ttatttattt 48600 tggcgttttt ctgttgactt gtcccaaaac tcatgctata ataaatatat aaagaataag 48660 gagaggtgag aacaccccgc aaacagggag ggaaccataa aatgaaagcg ctttcatctg 48720 ttatacaaaa tgagaaaatt ctaattaaaa atacagtatt ttattattgt ctttcctctt 48780 ggtctatgct ataataatta tagaaagtta aggagggaac aaaaaaaata aaataaaaaa 48840 aataaattta ataaaatgct tgacaaaagt tctaaggttt gctaatatat aattatagaa 48900 aggaacgatt gaaatgaaaa ataaatattt aagattgaaa ttgattgcta gaaagaacca 48960 aagagaaaaa gaaattgacc gaaaagaaaa agctgataaa atgataaaag tgttgacaga 49020 tgaattaagt cgtgttataa tagaatcaag ataaggaaag ggatgaaaaa aatgaataaa 49080 tatattttac ttgttaaatt cttagatgaa attagtttgg atagtggtta tcctaaaatc 49140 gaagaaattt ctgttgaagc tgatacttat gaacaagctg ttcaagattt caaagaagaa 49200 tacaaaaata gaaaatttga aattatttaa caaaaaagta ttgacaaaac attttagtaa 49260 tgatatactt aatatataga aaaggaaagg aagttttaaa atgaaattat ctgaattagt 49320 attattaatt gtttttgtag gaattttggt cgctggaggt atcttactac cattctactt 49380 tacttatcaa gctattttat atactggatt tttctctatt caaagtttgc tacttattgc 49440 tctaacaact attacaagtt atagcgttgt tactttttta aaagaaaatt gggaaaaggc 49500 ttgacaagcc tttctcaata ctctataata agagtataga aaggagttga aataaatgaa 49560 agaaaaaact ttctctttaa aagaagcttg ttggtattgt agtcaactca cacaaacaga 49620 tttaaccttg tggcaagttc ttattttgga atctttaaaa aataaaaaag agtataaaga 49680 aactgaatta gataatttaa tttctgattt tgaaaatgat ttaatttaaa aatttcttgt 49740 tagaaattag tataaaacac ttgacaaaca aatcaaaatg ttttatacta agaatgtaat 49800 aagaaattat tacaaaaaaa tattttaaaa aggtggaatt tatcatggaa aaacaaatca 49860 caaaagcaca agcattggaa ttagcaagta aagtagtaag caaagcaatc gaggacaaaa 49920 cttttttaaa tactgactta tttgaaaaag ttgatattga attactaaac aaattggaag 49980 ctatgcacgc tcaacaagtg aaacaagaca aggcaaaacg tggcaaaaaa ggcgtaagca 50040 aaacagcaaa agaaaaagct gaaaatatgg acaaagtacg aaaagcattt cttgacctag 50100 aaccagaaac agccttgaca ctgaacgaaa tttctgaatt aattgggctt gttggtgcta 50160 cacctcaaaa attgacggca atcatgaagc cattagtcga caacgaagaa tttgaaaaag 50220 ttgtaaagga taaaaaagta gcttacaaat tgaaataagt tagtaaaagg gcttgacaag 50280 ctcaagccct tttgttatac ttaaatagta gttaaggaac aaataaattt attgaaaagg 50340 tggaaaatta catgcaagaa acatttttga ttttagatac tgaaacgaca aaagagggcg 50400 agggaatgcc ttatcagtca gtttttgata ttggttgggt tatttcagac aaagggggaa 50460 atattttagt agaacgttct tacatggtgg aacaattcaa atttcaggca ttaaataaaa 50520 aacgtgcttt cttaattgat gaaaatgtag ttgatgggaa aatctatttt acaaaattac 50580 ttgaaaaaag aatcattgtt agcaagtggg cgaacattat cggacaactt aaaaaagatt 50640 gtaaaaaata taatgttgct tacattggcg cttacaattt aggttttgat actcgtgtaa 50700 ttgcaagaac ccatttcttt ttttcgggaa aagattttga tttctttgat aatttctttt 50760 tgattgatat ttatccagta gccgcttata ctgtattgaa tacgccagag tataaagagt 50820 ttgcaaaaac tcacaacctt ttaacagaaa aaggaaatta ccaaaccggt gcagaagcaa 50880 cttataaatt tttgttcaat gatacgaatt acattgaaga acataccgct ttgcaagata 50940 gcatagatga aacaaaaatt ctgcattttc tattaaatca agaaaaagaa attcctattc 51000 atgcctattc tattaaccct caagcgtggc gaattgtgaa tgaagaaaat ttaaataaga 51060 aaaagagttg acataactct ttttctttgg tatgattata ttttaggata gcttaaaaaa 51120 gctatctttt tttgtgttga aggcttgtga aatagtgagc aaatttgccg ttttcgggcg 51180 gaaattttac cacaaaatta ggtctatgtc aagaaaaaaa tcaaaaattt ttctaaaagt 51240 ttaatgagaa aaaatatcct aaaactcatg tatttattat ttgatttttt tagttttatc 51300 tgttataatt gttatagaaa gttaaggagg gaacaaaaaa ataaataaaa taaaaaataa 51360 tagttgacac gaaaaaattt tcatgttata ctatgtatat agtaaaggaa aggaatggtg 51420 ttatatgcaa gaaacatttt ctaatggcga aaagattact tctcatgatt ggaaattaat 51480 tgatgaaatg gttgaaaaat tagagttgaa tcttgccgaa gctattgagc tgagagagtt 51540 cgataagttt gaaagtgaca aaaaaacaga cttcgaaaaa gaacaaaaag aaatcaagaa 51600 agccaaaaat gcaaaatcaa aaattacaga tgatgaaatt gatgacctat ttcacaatgt 51660 agtaaaaatt gcttttggtg ggaaagagta tttccaaaat aaggactttc atttgttagt 51720 tgctgataaa tacacaaacc gccagacacc tagcagattg aaaaaaatga aagaaaaggg 51780 atatttagaa gatttgggcg gaagtcctaa aaaatataag gtaaagggtt gacaaaccct 51840 ttaccacctg ttataataat aatataaaga ataaggagtg aataaaatga gattttttaa 51900 aacatttcaa gaaaagaaat tatatttaaa aaagtcaaca gatgaagctt gtatcataaa 51960 aacgaaacta aacttaatta ctaaaaaatt aacagtcaag gtttatttta ggaatgcaat 52020 tgtttcaaaa aacaaattta aagttgacaa tgaatatatt tataaaataa ttgatttttt 52080 aaatgatgaa aaagctacag aactgactga attgtgctgc ttttttgacg gtgtgaagta 52140 ttatgataac acagaaaatc ataggctttg gaattttttc catgtatttg aagtagaaaa 52200 aaatatggaa aaaattataa caaacgaaaa aattaaaaaa atataagggt tgacttttca 52260 gcccttataa tatataataa gaatatagaa aggagttgga tcaaatgctt ttgtttacta 52320 aaaaagttat tatcacacaa cacgccaaag aacgctactt agaaagagta gccaaaaagc 52380 caatgaataa taggcaagtc cgttcggcaa ttcttaacga ttttcaaatt aagaacgtaa 52440 gaaagaaaac gccaaaaaat gaacagaatg aatttaaatt gtgggttttc ggttctcgct 52500 tatatgtttg taatgaaaca gataaaacta tagtagtaaa aactgttatc caaatgttac 52560 cagaggacga aaaaagaatc ataaaagaaa ttgaagaaaa gggttgactc ccttttactt 52620 acatgttata ctatatatat agtaaaggaa aggaatgaat ttaaatgatt attttaagta 52680 ataaaaagat gtatcaaaaa ttagaaagac tttgggaaac aagaggaagc gaaaaagaaa 52740 gctttgagga tttccttttg gatttatgta atgagtatat tattaacacc gcagagctaa 52800 aaaaaattag tgattatcat aaaaacaaaa gatgagattt ttctcatctt tttttaattt 52860 tgcttgtgaa ttttttcaca aagaaatacg tgttatttta aaagtgaagt ttgtgaaatg 52920 tataaaataa aataacagcc caaaaggaat aagataaaat aacattgtgg ggcgaggttc 52980 ccaaaacctt gatctatcaa gctttcattt ccattttcat aaattgaaaa aggattttca 53040 aaaataaaat aacagaatcc tgaaactaaa gtttgtgaaa tgaataaaat aacaaataga 53100 ggacgttatt tttcggccgc tcgctggcga gcgccatttt actgtgttat tttatccctg 53160 tcaagttttt ggcttgttat ttaattgttt tttaatgtta ttttaatatc ccaaaagtcc 53220 atgcatgtga attttttcac aatccagaaa gcctattata tcaagctttc atagcctttt 53280 cattccaaga gagcaagaga atgtgaatgt gcgcacaagc cagagtgtgt tatttttctg 53340 ctcttgggtg ttattttatg tttgtgaatt atttcacgtg taatttttgc ttgttattga 53400 aacaacaatg aaaatttctg aaaatctttt gaaagcttga tctgacgcag tttgtaagcg 53460 cttagaggtt tataaaataa catgtgtccg aagaaaagct gttatttaat tataaaataa 53520 cgcctggtga attgtgtgta aattagaaac aattctcatt aaactttaat gatttttggt 53580 gtataaaatg ctttactttt tgtcgtttct cccttataat aagtatataa gataaaggga 53640 ggacgaaaga aatgaaaaat ttaattttaa cagatgtaga cggaacactt acaaggaaaa 53700 gcgttgtttt atctcatgcg ggttttttaa ttgaaaaagg aattatcaag gacgacggtt 53760 cttataaagc atggaaaaaa gatgtcaaaa atgaaagttt gatcgttgcg gttgccgaaa 53820 attatcgcaa tgagataaca ggaaaaacag aaaaagaaat gtatgcaaaa gagtttgttt 53880 ctcaattttt agaaaatgaa aaaaattggt atcaaacttt gaccgattta aaagaaaaaa 53940 ttttacaagg ctttgaggtt attcttgtta caggttcgag cgatttttta gttaaacatt 54000 tagctgactt tttaggctgt tcatattatg caacggaata tttgaaaaca aatgatcggt 54060 ttactggaaa agttaaggga atgttttcag aatcacaaaa agacgactgc attcaatgga 54120 acataaacac aaacgagtat agttatatag aggcgtgggg agatactgcg agcgatttcg 54180 gtttattcaa acatgctgac tatcgtttac tagtcgaacc aaccaaacaa acacttgaaa 54240 cactaatcaa aaaaatcaca attgataaaa ttatttagaa ctttatgctt gcaatctctt 54300 ttcaagtttg ctataatagt attagagata aggaaagaga ttgcaaaaag ttctttgaaa 54360 aataaataaa ctttatgctt gcaatttctt ttcaagtttg ttataatagt attatagaaa 54420 taaggaaaga cgtaaaaagt tttttgaaaa ataaataaac tttatgcttg caaagttaaa 54480 aaaagtatgg tatacttgag agggaggttc acttgttact ctttaagctt aaatgagaat 54540 tttctaataa agcaaaaaaa gacttgcaaa ataattgaaa atcgtttata cttaaagagt 54600 agtaagggaa caacttacta acaaaatttt aaaaaggtgg aatttatcat ggaaaaaaca 54660 caattcgaaa aaatggtcgt agctgaaaaa tttgcagagg ttgctcgtat cttgtcaaac 54720 gtgaaagcac cgcaagaagt tatcgatttt gtagtagaaa aaggcgaaca agctcgaaaa 54780 cgtaacgcca accgcaaacc tagtcaaagc aagacaaaca aagaaaaact tgcagacatg 54840 caaaaaattc aagaatggtt ttttgaggaa gctgatccag aaacatttta cacaagccaa 54900 gaagtcaatg aagctttaga gtttgactat acacctcaaa aaatgacccc acgcctaaaa 54960 ggtctagttg aggagggagt cctagaaaaa ggaatcgcaa caagcgacaa gaaaaaagtg 55020 ggctataaaa ttatctaagg aacgtctaag gctaggcgta aaagcctagc cttttatttt 55080 ttcaaaaaaa tattttatta aaaaggtgga tacagtaagc atgaaaattg acaaaagaaa 55140 aaaatacatt ttagttttag acgtagaaac agctggcgac gttgaaaaaa atccattatg 55200 ttatgatgtt ggctttgtgg tatctgataa aaaagggaat atttacgaag aacattcgtt 55260 cgcaatttca gaaatttttg acgatgatgc tttgatgcgt agcgcttatt actatacaaa 55320 attacctttg tatcgtgaaa aaatttctaa aggaattatg gaagtgaagc cgttcagata 55380 tgtgagaaaa gttattctta acattataga aaaatacaat atcaaaaccg tagcagcata 55440 caacgcgtat tttgattttg ttaaagcttt aaataacaca tggcaagaat tgacaaacta 55500 tccgtttttc tttccttatg aagttggaaa aaatttagaa atcaattgca tttggcatat 55560 ggcttgccag acaatttttt cacaaaagag ctttccagag tgggcgtttg aaaatggtta 55620 ttactcaaaa gctggaaatc tgaaaactaa tgcagaagtc gcttataatt ggatgatgaa 55680 aacaaccgat tttgtggaag aacatacagg gcttgctgat gtatatatag aaacggaaat 55740 tatggcatgg tgttataaac aacataaaaa aatgaatcga aaaataaata gaatgtgctg 55800 gcgtatccct acaaaagttc attctgaaaa aattgctgaa attaacatga aagattttga 55860 agcaagtttt caaaatcaat aaatgagaaa atggtgagag aaatctcact attttttaat 55920 ctatgaaaat gaaaatattt ttgaaagttt tcatgaaatt ttctgaaaat caaatttttc 55980 tcaaaattgt aaaattagag atttctaata atttttttgt tattttttaa taatagtttg 56040 tgaaaaatta aaattctctt gttattaaaa aaacataaaa taacagaaaa aacttaattt 56100 ataaaataac accgccccaa aacgttgata tgatgggctt ttcagctccc tgaattaata 56160 ttcaataaca tgaataaaaa cccaaaatcc caaaactgtt attttaattt gtgaaaatta 56220 gaattctctc ataaggggac gttatttttt ggccggtcgt gcacggccgg aacccagcct 56280 aatgtcgacc accacgctag attttttccc cgttaaaggc ttgcctcgta aagccaggac 56340 tttttcctaa aactcagcac cccttgattt tttctgtata aaatgttata ataaaacaga 56400 gaggataaat tatcttgtct aatacaaaaa aaagacccac gaggggtcct tacctttatt 56460 cttcttcttc agtagaattt aagttgtcta agatataacg agatacacta gtacttgctt 56520 tttcaaagat aattttgttt tgtccataag gctcagttga aataatattt ccaatagtta 56580 gatcatcaat tgcttcctca taggttgcag gttgaaggaa acctgccata agacaaggat 56640 tagtgaatgc atttttgcct tgaatagata ccaaagtata ggctcctgta gtgccactat 56700 aggtagctag cattccatac tcaaatgaac tgattcttgt aggggtttta tattgatcca 56760 gattatcacg taggtattca cgtcccatat atttctgaga agtatatgca tattgcatct 56820 ctacaatatg ttttttaacg tcttcatcaa atttcttaag tgtttcttgg ataaagtctc 56880 gatcaaaacc taggtcattt aatacaggtg aacctgccca accattttcc tcgatagaag 56940 taaatagttg tgaccatgac cagattgcaa ataagccaat ctgactctct agtttaaagt 57000 cagggtcaat cttttctaat gattttttaa cagagaatgg agctcctgca cgtacttctt 57060 cagatagata gagagggcta actatatctc caatcttttt gtctgttaaa ttattgtgca 57120 tatattgatg ggcgcctgaa atgactccac tttctttagg cattagatat tttaataatc 57180 ctttttgaat aattgcatat ggatctactt ttgtttctac tgtaagatca gtgctataag 57240 ataataaatc attgactact ccacgcagac gcattccaat tactttagct tcttcacgag 57300 tataagcttg aggatacaag attaaattag agatattatc agattgacta atattgatgt 57360 gatgtccatc aacatctttt tcagcgataa tatagaaacc agtagactca ctatatcgtt 57420 tccttccagt gattaatcct tctgcattag ctcgataagt agaaaaacct tcttgacctt 57480 ctgctactac catattacga gtcaatttat taaggattaa tcctaacgta aagccttctc 57540 cagatttttt tctttgaact tgtgcttttg gagtaccttc tataatttta ataacatctt 57600 cttgagttag tcgtgataat ttcataatct aaaatcccct ttcaaaattg aaaaacgaaa 57660 attgaaaatc ctaaatgctc ctcccgaaac tgggggagta aactaggggg acatatccgc 57720 cccctatgtt tttatttatt tttcatatct catttatata ttaattatat cattatgaat 57780 atattttttc aaacgttgtc acccaagaga gaagagtagg ggcttgtcgt tctttatata 57840 gtggggccgt tactctttgt ttggggctcc cagtagggat ctgttagctc gctcatagat 57900 atactctgca cttttggctt atcattaaca taatattcta atatgcgtcc agtgggggcc 57960 gataatgcac tgacgaaggt ggcgccctgt gtaattgcat ggagtctcat ctggtctaca 58020 ttccattcgt cggagcgatg agtttcacta gtgccatcat caaagtagca taccatttta 58080 cctccgtgga ttactgccat tttaagctgt ttactcaaat aaatcatcct ttctaggttt 58140 gtcgcgtatc gtcgtgtttg ctgtaaggga ggacggggcc agccttgcgt taaactgtga 58200 ctgggggttg ccacacaaca ttgagggttt ttgagggttt ttatttttga ttgagggttt 58260 tcatttcaat attgaggatt ttctctcgaa tattaagatt tttcgacgct ctcgaatatt 58320 ttttcgtata ttttcccgtc taatgtgtcc tctaggctag gttttgtgcg tcttaaccgt 58380 ctattacacg gattttttct tttttatttt tccttgtttt tttaccaaaa cggaggattt 58440 tactcgtgtt tgaaccaatc agacctaata tcttctgtgt ccaagaccca tctttttgca 58500 aaagaatagc caccagccct aggagtatat gtctctatat tagccctcca agcctttgtt 58560 ttctcaaaat cgcctttttg aaaaatctca tccctaaaaa tatccatcgt aggagaaatc 58620 caacctataa gattttcatt ttccgttatt tctttaatgg ggcctactat cttaattgcc 58680 attatccttc ctccaatact ttacttatta aatgaaatgg aacataggta gaatgatcat 58740 cgagaccgat aaaacaagtt gtaaataccg atttctcttt aactagaaca attatttgcg 58800 caatctcagg acagtaactt gtaagaggca ttcctgagcc gccccaatca atatcataac 58860 cttctaagga tgcaagcgaa gctaatagtt tcaccattac ccttctatta ttacctacat 58920 caaattgcac ataaaaaatg ccatcttttc tgcatctctt caataaattt gataaataat 58980 attgtaacat gctcatatta ctttaccccc caagctctag caaaaagctc atactgaagt 59040 ttacgagaga cttctttctc ttcacaagaa aaagcagaat ataaatcaca aaaagttaat 59100 acaaagttat ccattgattc tctttcagta caaagaatcg caactgatcc attcatttta 59160 atagcactaa aaagtgttgg agaagcacca gattgccatt taacatcgaa tttagattct 59220 tgcaaggctt ctaataaatc ttcttccaat gcactatcat catcaacata aaaaataatt 59280 ctttttctac cacgaactcg tggcacactg cttaatagtg aatcaatgaa atatttcctc 59340 aacattttca accccttatt taactttata attaattata acacgtaaaa ctaaaaattt 59400 caaataaaaa tcaccacgta cgaagccgcc cagtacctta tattttacga acgaagtgag 59460 taaaatataa ggctattatt taggttcccg aagggaacgc tatatattac ttagttgcga 59520 aatatgtctt caaaagtgct ttcatgtgcg agaaatatgt ctcgagaaat ttccaatata 59580 gctcattgtt tatacctgtc ggtttagctc aaattctaaa atttgatctt ttggtatata 59640 gtacgaaccg cccatgttag aatcttccat aattagaaaa tcgtctgtaa ttctataatt 59700 accaatattg ttatattctg tttctgtgat accgccatag ccattggatt tggttagtat 59760 aatagtgtac attattttat catcctcttc tgttacgtgt gatagaaaat atccgctgac 59820 accagaatca ttcgattatc actgaaattc ttttctagta atactgatac gataacgcct 59880 tctagttcat gcgtattacc acaagcttct tgtagatact cttctgcttc tgtttgagtg 59940 ctgaaaactt ttggactata agctaactct gctctcgctg gatcaataat ttctacaata 60000 tacattttaa ttcttcctat ctattataat ttgtggcttc tttttcatga tttaaatttt 60060 atttttgggt aatccattct aaatgaagaa atttgataat aaccaatcag aaccttcgag 60120 aataacaagt ggtggataag ttgtgtcaaa atcacaacgc tggtgaaaca ttcttgtctg 60180 gggattccag atgaaatgga agtcccctac tttatcgtcc atataaagca aatctggcat 60240 aaaaattgct acatatttca tttttcatta cctcttttga ttgaataatt aagacccaga 60300 ggattagtag gactatgttt agcttcttca tcatatagcg aaattgtctc cattcatatc 60360 acaaacatat aactttaatt tcttttcgtc ctctggtagg gatcctaatt gtttaattat 60420 ctgctcaatt gtcattttta tttccctcat ttttcatttt tgaaaaaact ctctactaaa 60480 gtacatgtat aggcttgaca attaagtaat ttaacatcag aaaaagatcg ttcgaaatct 60540 tgaagttcat caactacata accaaactct aactcatcat tagattcacc aaattctctt 60600 ttacaaattt tagcatattc tttaaaggta attggttcct tagttaaagg aatattcgtt 60660 gcatttgcta aggttcttac atatttaacc attcgttcat ctctaaccac caaaggataa 60720 gccattttac tagcatataa taaagccgta aaagtttttc cactacctct tgaaccacta 60780 atcattaaca ttttattttt taacatttta tttttcctcc ttattgattt atgtaattac 60840 tctataaaaa tctttatcaa ttactgaaat taagccttca gtaaaagttg gtctctcatc 60900 aaataaatct atttcaattt ttcgcagaat atatattttg tcaacaacat taatttctgt 60960 aataatataa tacttgcctg gttcagtcgt agaaccaatt atatctcctt tatgaaactt 61020 acaatttatc atagaaaatc ctccaattca tcctacaaat aaaatcatcc ccattaaatt 61080 tcctaacttt attgttaaat aaaaacctat tcctaaaatt attgctttta aaataaagaa 61140 atgcactaaa ttcattaaaa taaatgctat tccaagagga agtaaagata ttaagagaaa 61200 cgtcggggcg ataatagcaa taaagattaa caatagaaat tctatcactt atagaatcag 61260 ctcactttct acgatatggt cgtttccgta ttctcttaag aaattcttcg gctgtaaaca 61320 taaggtaaat atacccacaa aacattaatc ctatcacaca aaatatcttt aataacataa 61380 gattgtcacc ttattctaat aaatgtactt cataatgaaa tatttttcta aaaacaatgt 61440 cattttcatt ttctatttca gtatataatt taactattaa atctctgcct tcttcattta 61500 gagaaatatt tttaatttgt gcttctttag ggtttaattt tttaaaaata aaatctaaaa 61560 aattcatttt cttctctcca tttcatttcc ttatatatta attatatcat tttaaaaaag 61620 aaaaatcaac taagaatcac tcttagttga ttaatttatt acttatataa ggcataattt 61680 tttgaactgc aactgaatat aatttactca tagctagtcc aacaataaca acaatgacca 61740 ttccaatagc attagtcggt accaacataa gcgttccaac tatactaaac aaaataataa 61800 aaccaccaat gatacctaaa aacaaaacag taattaatgc cacaatttct ttaaccaatc 61860 acgccaactc cttttcttgc tcttttgggt ttctttttgc tgatctatta cttctttaat 61920 ttcttgaata agaggtgttg cagataaagt ataactcata ttcttttcaa actcttcaaa 61980 tgattgatcc atttctttaa ttagttcttt aattgcctta ttttctaccc gcatctaatt 62040 tctcctgttc atgcatttct ataatagctc cttctggtac tattaattta taactaatag 62100 tagtagcttc tttacgattt aaacttataa tatatgtcca tagggttgtt ggaattaaac 62160 ccactgctct ttctaaccta ggtctttttg ttctattatc tataataaca tcagtatttt 62220 cattcatttc tatgttttta tataattttg tattcccatc cacttctaat gtagcaaaag 62280 tataaaaatc aacttcatct gattctccat ttttatgaac aatatagtct tgctctgaat 62340 taatatatgt caatggggta actaccatag ataattcata actattagtt attttataat 62400 agcctaatgt taaaaatata gccactaaag aaacagcggt agcttgaaat atactcccaa 62460 tagcgacaga aaatagaaaa gttacgccct ggtcttcttt atcaattact tttcgtccgc 62520 gtcgatacat attgacaaat attgcagcaa atactataat aaaaaatatc atttactgtt 62580 tgccctccct tatttaactt tataattaat tatatcatgc tatatataaa aaatcaaatg 62640 ggtatctcac gacaaactgt cctaattttt ttgtatttca gtagagagga ggtcagaaag 62700 tttgaacaat cataaaaatc aaattccaac gacacaagat tttacaagtt taagaaatat 62760 tgatagatta tatggatatt tacaagtaat ttctaaatgg aatgggaaaa aaggagagcc 62820 gagatatatt aataaaaaag acttttctag tcgagaggct gcgaatgaat tatgtgtatc 62880 aagtcgaact ataaatcgtc aaattaataa gttaaaagaa tacggtttta ttttggaaga 62940 aaatgataaa atgaagcttc ctataaaaca tttatttact actattcatc cagaaacact 63000 taaatatcta tatcaaatac aaacagataa tgttatcaca atatattctt ttcttcgagc 63060 aatgcaatgg tattatcaag ataagaacaa aatcttcttc tttacgaaac gttatttaat 63120 tactgagata ttaggcaaaa aatcttcagg ttcagcttat gacgctatgg actctatttt 63180 agatttacta gataaattgg gcttagttaa attagcatgt caaaagaaaa aaggtaaatt 63240 tggagaatat aaggtatatg ctgttattga agtaaaagat gaaatcatta gaagtaattt 63300 tccaactatt gaagaagttt ttggggatat ctatattcct gacgaagaag aaattaatgt 63360 aggtataaaa gaatattggc aaaataaaaa agactagaaa aaatctagtc ttttttccat 63420 ccataaattt ttgtattagg atctgtaaac attttttcta attcgctcaa ccctggattt 63480 tttgatagta taactggttt tttatcctct tcatatcttt taaagaaatc aagtttaata 63540 ttgattccat caggaattgt aattgtccgt gggggcttat gttcctctag gctttccaaa 63600 tctaaatctc tcattatttt cctcgcctct tttctttttc ttgttcttgt tttactacaa 63660 gaaaatatat tgtattagct aaagcgactc ctaacattga aattatcaat cctactaaaa 63720 atactaaagc atatccaaat acagtaatag aagctagttg tgggaaagca aaagcaatta 63780 atgcaacaaa agctattcct ccaattaaaa ttggtccaat taaatgatta atcttataca 63840 taataaaatc aagatattct ctcaaattaa ttatcctcct ttggcaaaat tctatacgat 63900 tcaatacttt ttactatctt accttctcgt tctcgagtaa atttcataat ttctatgaaa 63960 aaatcattta attctacttt atgaaaatgt ttaactttct caggttctaa atatataata 64020 tctaaatcgc taaaaacatt tttattaaaa accaagagag caagaatatt gggattttct 64080 ttcaaaacta tatatggcgt tgaacttact gggtctgtaa ttactttcat tctagtccac 64140 tatcaagttc aatttgtttt tctaaaaact tatctaataa acttgggtct gccattcttt 64200 tgatgttata aaattgtaaa gtatctaaga aaaacgtgcg aacattcgca tatacatcag 64260 aaaaaacaac aatttggact tcttgctcaa tatctacaat agaataatat tctccatcaa 64320 tttgatataa ataagatgga tctaaaatga tttcttctaa ggaggcttct acttcttctg 64380 tcatatcaaa ttctacttga ttatctccta ctaatcttac aattgaatca agcaatcctg 64440 gttctacgtt atttaattct aaactaacct tcatatttaa aaacctccca attattcttt 64500 ctattgatat aaatagtttt atcgtcagag atagcaaaaa tttgtttatt tgcttctata 64560 actacatgat cgtgagcaaa cccagaacaa aaatttcctt tgtcataata atgtttgata 64620 tcaagacctg ttttttcttt aaagaaaaga agtttttctt ctagggcttc cttattagct 64680 ttactagaaa gccaattata atcttttttt atagccatat aaatccctct ccttttacac 64740 gttcttatac tctttatgaa aaaggaagga tttcttattt aactttataa ttaattatat 64800 cattttatat ataaaaaatc aaaaaaaggg tagcttatac taccccatgt ttacatcata 64860 tctattagca atttcttcaa aaaacgacaa taatcgttca tcatcccaag attttaatgg 64920 atttccttca gatttaaact ttttatcaag ttttttagct tcaacaaaag ctatatcata 64980 gaaattaatt ccatccatag gacttttaaa tactaatttt gtgcgggcat tcatttcttg 65040 ttgaaaggcg tatttgtcat taatcaatta gctcatctcc ttttggtgtt gattcaattg 65100 gtaaatcaac gtattcatta ggatcattaa tttttaaaaa tactggataa ttttgtcctt 65160 catagataac aaaagaagct acaaattcac cagggaagaa atctaaatct tctgcattta 65220 ctggctgtgg tacgacagta tgtttatcaa tatcatattt agctttattt ctagaaatac 65280 cataatctgt ttttaatcta tctttttctg ttggagttag tttccaatac gttaaatatt 65340 caacgtctac tgtattagtt ttatctgaca atgtgatact cctccttatt tttattatac 65400 ttatattata acatatatct caaataaagt caagaaaaaa agccctattt aacgggctta 65460 gattttttat gttctttata aactttactt ctaaaggttg cttcctgttt tttcaaccat 65520 tcagtaaaat caatcttttg tgtcaattca actggttctt tagtagtgtt ttgttttttc 65580 gccatattta tatcattctc ctttaaattt aatttttagt cgttagattt tttattaatg 65640 aaaattgctt ttataaaagt aagcaataat gttatactaa aacattggaa aaaagttaat 65700 gtaaatagtc ctggaaatgc tattgctact acaaaattcc aaattagata taatacaatt 65760 ggagcaagta aaaccataaa tgtaattatt gcgacaacat atagaaaaaa tgctaattta 65820 gcaccgaaca tataaaaacc ccctattttt aataaaagga gctaatggga ttcgaaccca 65880 tgagccaact ttatgtcgac taacgcatta gcagtgcgtc accttcagcc tctcagtcat 65940 agctccgtaa aagggaagat taacttccca aaaatcttat agtattatac catttttccc 66000 attttttatc aactaattct attgcatctt ccagaagaag aggttctcca aagttagaaa 66060 tctttgaagt aatttcagga gaatcaatgc ctacattaat catattagcg tcaggggcaa 66120 tttgctcatg aatatgccca caaattgaac gcatattttt acgttgctct cctaattgaa 66180 atggatagtg agttaaatag taaactttac cattcatttt ttcccttaaa ccaacttctt 66240 caactaaaaa tttaactctg tcaggagatt gtttacgttt tttaggatta ctatatggat 66300 aatgataatt attatttaca atatacttaa ataattttga actatcatga tttcccttaa 66360 ttaaaataat ttgtccattg atcttttcta atgtttcaaa caatttttta ggtttaccaa 66420 aaccaatatc tcctaaatga ataatagtat catctctacc aaccttttga ttaatagttt 66480 taataatatt atcgttcatc tcttctacag aggaaaactt ttttctagta gaaatgaatc 66540 cttctggacc acaagcatta ttatggaaga aatgccaatc actcgtaata aatttagtca 66600 agtttttccc cttctttcat aagaaataag tccctggtga gattcgaact cacgtattat 66660 gggtttgcaa tccatagcct taagcctctt ggcgacaggg acatttttaa agttcttaat 66720 taactttata attaattata acatgattaa tatatttttt caaatatgaa aaagcccaag 66780 agagcgaata tagtgttggc actttcctgg gctggattaa tgcttcctcc tggaatcgaa 66840 ccagggacct ccagttcttc agactggcgc tactacctac tgagctaaag aagcgtaact 66900 acctcaatta agaggtagaa aagggggcat tttagatggc ttcatcaatg aagtcaaatg 66960 ctggattaga taacgtttct aacatcatag cttctggagt ttctagtttc ccatttaaga 67020 tggaagttag aatatttgtg ctgaatccag atactaatgc ggctccttgt tcattataac 67080 gaacaggcaa gtttcctaat ctagcttcta cattccaaaa gactacatct ggcatttcat 67140 aaccagcgtc ctcaaaacga gattttgcaa gtttgaaatg tgtaacgcca tttcctcgtt 67200 gaaaatcaaa ttccatatct gaaataatga taatcttaga tggaacttct tcttttggag 67260 tgttattctt gatagcagta tctaagataa gattaaatac tgcttcaata tttgtgtttt 67320 cgataataga atgacgagaa tattcattca ttttatctcg taaggagcaa tcatttggta 67380 aagttactaa ttgtgggcat tcagagaaag tgataaaatt attcttgaat ggtccttcca 67440 agcgttcagc agtataaatt gccaagcctt cagctacttc ccatggttta ccccacatag 67500 aagcagaagt atctgcaact acaattacat tctcgtcaga acccttaaca gtatctttta 67560 gagatttcca tgcagcttct aattcaggtt gttcttcata agaaatacaa ctacgatatc 67620 cataagcttt aataatttca tctggcatta ccaaatttcc attcattgta gcttcaccat 67680 tatttacagc atccaaaaat tcagaataac gatctgaaat atggcgccaa aatgctgagc 67740 gatacttaaa tcctgctcga gaaggtagct tagaaaaatc aattgaatca taatctttag 67800 cagccaattt tacttctact aaatctaatt tttctcttag attaaccaac atctttcggt 67860 aaaccttata atcactaggt ttagtatatc ctaaagcatt agcaactcta agaccaaaag 67920 ctcttcgttc ttcgttttta gcattgatat taggcaacca ttttcctaat aaagaaattt 67980 ctttgccctt tcgcatatta tccatatctt tcagaagctg agttttaatg ataattagtc 68040 cataatcaaa caatttttca tcttgtttca taatgaaaaa tagatcatcc caacgaccaa 68100 aatcagctac attattcatg ataacatgaa gtgctttttc atctttcttt ttagctaaga 68160 accaaattcc tgcgtggaac atatctcttt cacctagtcc accattacga atatctcgca 68220 tataaactag atttctcaat gcaacagctc tatcttcatt atatgctttt tcaaataatg 68280 aaaccttatc tccaacactt tggtatcgca tagatccacc taatgcaaaa aagtcaacat 68340 tagcatttag tgaagtagta taggtagttg caccattaaa agtgcgtcca actgttgctt 68400 cctctttcaa attatctaaa aatgtcatag ttatttatct cctttatctc ttaatcggct 68460 aaaaataact tcaagaatct tatataatat atttgaatta gcctattaaa catatgatat 68520 ttgctgtaag attctttacc taagcgaaat atgggatttg aacccatcta aataattcta 68580 cctgttgaat taatttcgca cctgtataag tctttaaaat aatttacatg tcttttaaga 68640 cattttgtcc taattacaaa gagcttcctt actttgtata gtttatagta gaagtggggt 68700 tcgaacccac gctctctcac ttataaggtg agtgctttaa ccaaacataa gctattctac 68760 tgtgaacatc gctattagga ttcgaaccta aacttagaaa ttagaagttt cttgtgctat 68820 ccattacact atagcgacat attaggaagt tttttcttcc taacttatat aaatattata 68880 acatgttatg ctaaaaaagt caatttttat tttttttgat taaataaaca ttatataagc 68940 atattaatcc aaccaaaact tttaagaata tagcaaaaac ttcaaaagag aaaaaggctg 69000 aaacaatata aagtatacct gcaacataat aaaatttaat aattatatca atatattttt 69060 ctctagtgct tggcttaatg gataaaattg tagcaataat aagaaaaatt aaaccaatcg 69120 ttcctaatat aactcctcca tttaacaata aaaatacaaa taccattaga ttacctccag 69180 ttcttcgtca aaaaaaggac aaatcatttc agtgccttct tttgaaacaa aacgtacttt 69240 attgaagact atatttattt cttcaattac tccaatacat cctttacaag caaaatcttc 69300 aaagtcttta agaataagaa cagtgtcccc tactttcatt tatctaacca actttcttca 69360 tcttcaaaat tttcttcttc aacaactttt taatctcatt aataatatta aatatgatta 69420 attatcttat aatataatta taatatatta tattaataat ttcaaattat ttaataatac 69480 gatatagact atgagtaaac ttaccatcta aacaattata tttatttggg tttattacaa 69540 tatttttact aataatccca tgcttttggg cattacttaa ataataatac atatttttca 69600 taccaattaa attatctgtg tgaaagataa tagttttaat attgtccatt ctagtagatt 69660 ttactaattc ttgaactaat tcgtacccat tcaaacagtt ttctcctagg tcatgatcca 69720 atgccaaaat atcaatttct acatcattat ttttctttct ccaatcaaga aaatcttctg 69780 cgctcctaaa taattcatat ctttcaggac acttccttaa atcatctaca aatactttca 69840 tagtatagtt tccctctttt gttcccagta ttcagataat tctaactgtt cttgcttaat 69900 tctttctttc tcttcatccc caatagaggc aaaatataag agaacttcat tttgataatc 69960 tgttaattct cgtttgatac gctgaatttc ttttttgaat tcttctaaat ctacttcttc 70020 ggggagcatt ggattttgta ctacgcctct aattaattca gttttggcag tgatattttt 70080 agctttatga aatattcttt ctctttcatt catttttaaa atctccttta tttaacctta 70140 taattaatta tatcatttta aattaatttt ttcaaataaa aaactccctt atttagggag 70200 ttttattttc tcataagttt ttcataaaaa tcaggatact tttctttggg aaaatatcct 70260 tttatcattt cataatccca atccaaatct acatgtcttt tatataaact tggaattttg 70320 gcatcttcat catatcgacc ttgtttcatt cttcttcgat aaagacgatt tacttgtttc 70380 ttggattttc tgacagaaga attcttcata caaacatatc ttgaatatgg ttgttttttt 70440 ctgcttctac tcatactata aatacctcct ttataagata tttatagttt attcaatctt 70500 ttcttcatat tgttcaagct ctcctatctg tctaataaat tatttaattt ctttgatttt 70560 accattttta aaccatttag agtttgtgaa tgctaataca tcatttgcca gatataacat 70620 gtaagttagc attagtacat aaaccgcatg tccatgtaaa gctgtttgaa tccatagagc 70680 taccgacatt agaccttgaa aagtccagaa gtaatattgc gctctaaagc ctttcacaca 70740 cagtacagct ccaactaaac cgatcattgc acttgttgca tctaagaaag cctgcggtga 70800 gtgtaaaaca actgtatcta agaagtataa agcaacccag aaaacaacaa taagacctaa 70860 tgttttaagc atgtgtacag gttgcatttt acgaggtttc aaaccctcat tccattgttt 70920 agagaagatg ataggaatgt ctaacaaaac aatataagca gtttgcataa taatatcact 70980 atagttacct gttacagttg ctacaccgat taataaaata gcagaaatga atcctaaaat 71040 accattaatt gatttaccca ttgtaattgc tacagtacat gtaaatccaa taattcctgc 71100 aattgtagca gtaaaccctg tcattgttaa cccttgacct tgtagcatag gtagaacaat 71160 accggtaaat acttggactg ccattccgac aaaccacaat acacgagctg atttgttcca 71220 accagttaaa ccatctttat aatattcgta agtgaataat ttttcaatat tttctttgtt 71280 atccttccaa aactttttta acttgtccat ttgttatttt tcctccatta aacagctttc 71340 tctagtgtgt ataagtcttg attgtcagta aacttgctta attcatggaa aggtagttta 71400 ccctctaatc tattgtatag cttcaatgca acttgtgtag cgtggtcttt acaccaaggt 71460 aagccattta actcacataa acatttaacc gtatctacag tagatagtgc cccgacttca 71520 cacaacttac tcaaaataat tgttaacaag taaacacggt ctcctttatc attcacaaag 71580 tctttatctg ttacatctaa aacaatttta gggaatccca tatctttttg taaccaattt 71640 tgatagttct cgtgatggtg tttaaagtat tcgtataatt cagtaccctc ttctactttc 71700 tcaaactcac gagcacgtgc attgattcgt ttgatttctt cttcaaaaga acaatttaat 71760 acaatcatta aatcgggagt ctttttaggt aatggttcta attcttttaa catacgatta 71820 agcaatcggt gataaactcc ctgttccatt tctgtgacat gaccctcttg tactagttgg 71880 tctaagaaga tactgtcttc aaaaatagaa cggtctaaaa taccgttatc tacactcata 71940 gcttcttgaa tcatttcgaa gcgtttagac aacatatcta tttggaaaag tagtccatac 72000 tttgctttat cttggtagaa caattcaagc attggatttt cctctaccgg ttcataaact 72060 gcttttgtat ttaaaagatt tgctaatatt tctgccattg atgatttacc tacaccaata 72120 actccggcta atgtaattat cataaccatc tcctctaact tttccttatt tttgacaata 72180 ttaagagcct gactggcagg ctctaataaa tcctaatagt attgaaaacc aagagaagga 72240 agatattcaa atccttctct attgggacgt cagtgtgaga atctcttaac gtttcgtata 72300 agcgaaatat cattccttgt actaccgacg atagaggact tgaacctcta ttactagatt 72360 cgtagtctag cgtcctatcc atttgaacga atcatcgaaa aataaaatga attacacaat 72420 atctagcacc cgtagataac taaatatccc tatatctatt ttagacctaa ccaatgcctc 72480 attcatttat aaatgctggt tgagagaatc gaactcccat ttgaagatta caagtcaact 72540 gtaatagcca ttatacgaaa ccagcgtaaa ggctgccgct gttttatttg ggtaaggaca 72600 ctgcgcttgc ccttttacga ggtgtcttta tgcctattgt gcgcctcaac ctagccgcca 72660 aagtggaaat ttgactaggg gctaatttta atgattaact tacgcaaatc ggaaagacag 72720 gacttgaacc tgcgacatca gactcccaaa gcctgcgctc taccaagctg agctactttc 72780 cggattacct gtttaatttg gtcttttaag ctactacaca caggtaaaga taaaccctta 72840 gcagagggaa gactatcatc gaaccgtatg taggttgcga tgggcagtag ccattataaa 72900 aaacaagaac ctatatatta ttagatatac tattatccca ttaaatgtga ttgctgttag 72960 gttctttata cggatagcag gagtcgaacc tgcatgagag agatacttct cgtcagatct 73020 taagtctgat gtgtctgcca atcccaccat acccgtgtta cctgtccagt gacatggaca 73080 ggaagaaagg aaagttgtgt ttatttatca agaacgaagt ttagaccttt ttcgagttct 73140 aacgtaaact caagaaaagc acctagaaaa aactcaaacg ccaaccatag ttgaactcgt 73200 agatttttcg gttggtatct acggctcctt acataatctc ttctgtaata tttgaataag 73260 gttttgctgt atcgttcttt atgacgctac cgagattcga actcgggatt ctggttagaa 73320 agaccagtgt cttaaccgct tgaccatagc gccaaaatgg aggacaaggg attcgaaccc 73380 ccaatgtatc ttacgtcatg gttttacaga ccacttgctt caccaatttg cataatcctc 73440 caaatactgg ggaggtacct tccccaaaaa caagaatctg ctttttgtgt gctaccatta 73500 caccatataa gttgtaccga acttatacta ggggtcgaac ctagacctta tttttctcat 73560 caaatattat taaaagtatt aatatttgct gtaagattct ttaactcacc cagcaggatt 73620 cgaacctgca agaattgatt aacagtcaat catgatacca tttcatcata ggtgaatgtt 73680 taaacgtgtt ggaataaatt ccttcacgcc caacgttcta acacgttgtt tcaatcaggg 73740 tagctggatt tgaaccaaca atctctgcat cccaaatgca gcggattagc caaattctcc 73800 tataccctgg tgtatcccca ataaaatatg gggatattta attaagacat tttgtcctac 73860 ttttcatcag aaactaattc agttcctttc tttttatatc caacatgatc tttattgttc 73920 gtagcttgac ctactttatc tacttcgtct ccaactaaac gcataattgc agacattttt 73980 tgaggagaaa cttcgattcc aactccattt gcaatttcgc cactgtcata agcgattcca 74040 tcttcagcgt cattgtggaa aaactcaatt actaaagcag cgatgtgttt attttctttt 74100 tgaaccttag tatcaacgtt tcgacgactt gcgttacgag cttcaattct ttgagcatct 74160 tccaataaca tttctgcata ttcaggagca tttacttcca acaattttgc tgcgctacga 74220 tacatatctg ctttagtttg tttttccatg atgaatctct ccttttaaaa taagtttttt 74280 tatttctttt tacatattaa ttataacatt ttgaaagatt tttttcaaat atcttttaag 74340 atttaactac aaccaattct ttatcaacta gcaagccacc acaatcaaca tgggtataac 74400 gattatttaa tcgtctgttt gaaaaattag aagctccgca cttatcacat tcagaatgaa 74460 ctttaaatga taaaagaggt gttgttggag ctaattgtag ctttttatta gttgctcctg 74520 acgaaagtga atcaatataa gacaacaatt tttcaaattc aggctctcca tcgctgtatt 74580 tacgcccact tttatgtaag taccagtgag caagttcgtg tttcaaaact ttttcaaata 74640 aagattcgtc ttttgcaaga tatagagcga tctttgtaaa ctgaccagac atttcgattt 74700 tattttcact agggaaatat ctaccatatg ttctagtaag tcggttattg ataaaaaaag 74760 ttggagcatt tgtctcttga ctagagaaga attgatcaat gattttatct cccatttcat 74820 tgatcttttc taaagcttca attttctgtt taccactaat agttcttgga taatctaaac 74880 taaaattccc aactatagtt gacattgtac tcctcctttt aataaaatta atgacttgta 74940 caggatttgc acctgtgatt cccgagtgaa agtcgagcgt cttaacttct tgaccaacaa 75000 gccgtttcat atttcttatc tttattatat aataattata acatattatg cttaaaatgt 75060 caaattattt ttccctttcg ggataaggac tggctaaaat gaattagctc aagtccctgt 75120 ttaactgaga tagtaggatt cgaacttaca ctgcatcgtc atgcataagg tgaagggata 75180 gcgaatcctc atattcttga ggttggactc gaaccaacgt ctcatggaag attaaaccat 75240 gcgtctttcc tttttgcgac cactcaagta actttatctt atataaatat tataacatgt 75300 tattcatatt ttttcaagtt atgagttttg accagtcata tactctgtca aaccatcgaa 75360 aacaacttct ccatcactta cagccaaaaa catctgacct gtttcaaatg gattttcaat 75420 aataattgta tatccactac caagagtatc ttgtaaacct aaagatgttt gttgaacatt 75480 ttgaaccaaa ttagtccata cgtctaaact taatgtgcca tccatgatag ctgccatttc 75540 ttgcgtaaat tgaggatctg ttggtgtaat aatataatat ttttttacag aatcaaaacg 75600 aaccggtcct gttgattgga aactttgttg aaaagcatct aaaacttgtt gttctaaggt 75660 agataattga acctcatctt ttttataagg ttctttttgt tcagacgatt ctactgtgct 75720 actttctact gtctgttcag tagaatcttc gcttttccat tgtccatcag gaacttctcc 75780 tttagtacat cctactaaac ctaaaccaaa cattgcaaca attgagaatc ctactaaact 75840 ttttttcatt taaaatttcc tccttattta actttataat taattatatc attttatatt 75900 ttttatttca aatctgaata tttttcacta agttcaaata tatcttcaat actaaattct 75960 ttattatctt taaaccgttc ttcaaaaata ggatttttca tacttttcct acataaatcc 76020 attgtctgtt cttccaaaag aggaagaata atagggttca agtgggagaa atccatattt 76080 cttaaatatg aactgctaaa ggaattagta aaaattcttt ccgccccaaa tgcacagtat 76140 cgaagaaata tagcttcgga tcgaggacca tatcttgatg gtgtaaatct atcagactta 76200 tatttatata attcttcgag agtgtgttta tctattaaat ttaattttaa cccctgcaat 76260 acagtatagt acgttatttt ttgtaactgt aaatgacaaa tatatttttt atgttctaaa 76320 cttaaatcta caatatgaga ggctaactct atcattttta cctccttatt taactttata 76380 attaattata tcattttaaa ttaataaatt caattaaaaa atgccctttt acaggcattc 76440 tttaattttt ttggcaatag attctaccat agaattatcg gaatataaat ctcccttagt 76500 aagataatct tttttttggc aaataaaaac attttctaat cccaaaaata ctttgattaa 76560 gttactatat ctctgctctg gagtatcatc ttgaaaaata gcatcaatag tatacccctg 76620 taaatctttt aataataaaa tatgtttaac ttgctctaca ctaaaataat ccatcattat 76680 ctccacgctc ctatatttac atttctcatt aaattagcga ttgtcaaagg tccaactcca 76740 cctggtactg gagaaagatt gccattccaa ccttcttttt tcaaagaatt agagtctaca 76800 tctccaacta acttcccaga ttcatcaaaa ttaataccaa catctataac agtagcatta 76860 tctttaatga aattgctgtc tataaagtta gcttttccaa ttgctaccac caatatatca 76920 cattgtttgc tgagagaaat taaattcttt ttatcagtat aggaattcat actaatgaca 76980 gttgcatttt cttccaaaag aagaggaata agtggctttc cgactaaatt ggaacgacca 77040 ataataccta cagtttttcc ctctattggg atgttataat attttagcat atctactacc 77100 ccatgcgctg ttgctgggac aaaatttagg tcagttctgc ttccatctag tagacgtcct 77160 aaattataat gtgttaaacc atcaacatca aattctgggg gaattacacg caaaaatgta 77220 tcttccataa ggcgatcaaa ttcaaaagga agttgaacaa atataccaga tactttatct 77280 cgattaggaa tctttgttaa agatacaagt tcttttaaaa ttttatcgac cttttcttta 77340 gtttcagcct tgtgtagctg agttggaata cctaaagatt cagccttttt aatcttattt 77400 ttgatatagc ggttagattc ctctagatct ccaacctgga aaatatgcaa aactttttta 77460 tttggataat gaatatcttt ataaattgaa tctgctaccg gtttaccata taataaatta 77520 attgacactg ttttcctcct ctttaataaa tctataacta ccatcactat tttttatcat 77580 agatatatta tttaatcttt ttctaacttc ttcaattttt ctttgttctc cttcagagta 77640 ttcgtgagta ccatgaatct tcgccatcca gatttcatat tcttcttgcg tccaatagcc 77700 aattgtttcg cctccacttg agatcgtata ttttatatct ttatccatta atcccacttc 77760 cctttatgtt taggctttct tttatacgag cctttaccct tttttggctt aatagttcct 77820 actccacgag aaaccttttg taggtaatct cctacttcat ccaatttcat tttatgagaa 77880 ggtttaattt tcatggaact ccactctcct ttatttttat atttctatgt aaaaagggaa 77940 ttataaaaat tccctttcaa tattatatgt tatttaaaag cactagtaaa ttttagtgta 78000 cctaatttag ctgcttgtgc ttcaatatca tttaaagcat caatacgatt agtaaaatca 78060 gaagttacta agatatcaat tggacgagaa atttcttgaa cctcagaagt ccaacgtcct 78120 tttgaatctt ttgttcgcac tacctcacga gcttttttac ttgtagagac gactccataa 78180 aattttgttt tcttcataat gtttttctcc tttttatcat ttattataat aatattatat 78240 catcttattc tgaaaaaatc aaattaaatt aaattcgagt tttacaaaag tctggttata 78300 ggaagttctt tccagtgaac tactctccac ttagctaaac ctaacggttc ttaacacttg 78360 aagtgggagc ttcttgggaa tagagcatac ttgatagctt atttcttcac taacagcggt 78420 ttctcttatt taccaagcta tccccgtagt tcctacggtt tttttgattt atttttatcg 78480 tcggtcgaaa tactaaccac acaacgttat gacagattca gtttgttttt cactctaatc 78540 ataatcattg ttacactcgc atcgtcacga atgtcttcaa gtcttacagt tttacttgtg 78600 gcgctaactc aatatctttc acttgagcgc cttcacactg gaattgcacc atcatgcttg 78660 gaatcaaact aattatagca tttatctttt cggtcttcga gttaccaacg gtaaccctca 78720 taccgaacgg cattcatcac ccacctatag aggatgggcg acttctgcct aattccatta 78780 aaatatatcc aataattttc attatattaa tctcctctta taacaattga aattttttgg 78840 tgaaatctac tagattgtct ttcggttgtg gaaagaaacc tacaacggtt agtgaacctg 78900 aaggaatctc agttaagcca ttatctctaa tatatactgc tcccatatca attgccttaa 78960 gcaaatcttt ttctttggct cgtaagataa tttttgtttg agatccagaa taaagccaat 79020 cataataatt attggttaat tctttttctt tagcaaaact atccatacta tcttcgtcaa 79080 gaatctcttc tgatagaact aattcaaaaa ttttattatc aataactgtt tgtacatgag 79140 caacttgacc agcgatttta ccaacgctca tatttaattc cttattaact aaaatatatt 79200 ctacaatttt ttccatttta ttatctcctt tttaagtata tattaattat atcattttat 79260 acgataaaaa tcaataaatt cacaatcttc ttgcttttgg aaactcttca tggtataatt 79320 attataatct atttcaggaa aatacgtatc tgcttgtctc ttttcataaa atcttgttaa 79380 atacaatcta ctccaataat attgagtttg tgcatataac cttcctccac caataactac 79440 aataacatct gaggataatg aatgaatctc caagagagca agaaacatgg actcatctat 79500 aacttttaaa ttgtcattgt ttttcatggc tgtctggagt ttttttgata ttcctctttc 79560 catatccagc actactgtat tcatcctatt gggtaatgct tctactggca gtgattccca 79620 agttttatac cccatataaa tatttctatt ccaagttaaa gttttaaatc ttttcatatc 79680 ttctgaaaaa tggtaaatta atttattatt taaccctaat tctccatttg gtccaattgc 79740 tgctacagca taaatcaata aaaacacttc ctttttttta ttctatttgt tattattata 79800 acaaatttat gtataaaaag caaaaaaata cccttatttc taagggtatt tttaatctta 79860 tttttatagt ctataagttc cccacagttt gtctgtagaa gtttttgtat caatttcttt 79920 tgcaggagca taacggctaa taatgttaac agttttacct ctggctggat gatttgctgc 79980 taatgcgcac caattatgga agcgacagtt accacgttga gcgatttctg gaccatattt 80040 cttagtgata tctggagaag ctaagtaaga ccctcctgta atatcgaatc gtacatattc 80100 ttttccagtt tttgcatcag ttacaaatag ataatgttta aatccatctt tttgacgtgt 80160 atttaaatgc catccggcga atgtaacacc tttagcatct ccagcaactt tatctaggta 80220 tccttcatta attaatgcag gagctttcca atgctcttta gtgcgaattt cttctttgtt 80280 atcgttgttg taacgaagta ataaatcaaa atcttttcct agaacacctt ctggcgtaag 80340 agcatctact tggaaaccaa catctgctcc gttaggattt gaaactgctt tttgaacgtc 80400 tggacgttga atttttttcg ctttaaagcg ggcaatttct ggtccacctt ttttcataat 80460 ataaacccaa atatcttgac cttttgtagc cttagctggg gtaaaccaac ctttgatttg 80520 gaaagctttt gaattcatat taactaggtc aatattatat tttctagttc cagtacttgg 80580 tgctggagct ggcgctggag ctggtttaga acccatataa gatttaatac gagcaataaa 80640 ataatcttgc attacatatc catctccatg tagtgcaaca gagcggtgag gacaagaagt 80700 tggtgaaaat tgtttatgca atttcacagt atttctatta ggttgtaaac catatctctt 80760 taagatttgg gctgctaatt taatagaatt ttcttcattt tgtcgaaaaa tattttctgg 80820 tcccattgat tgacatgctt caataccgat ataatatgca ttaccagttg aatttgcagt 80880 atgccatgcc atattcattt catcttctgc ttggaatgtt ccatcgctag ctacataata 80940 atgagcaaaa ccattttctg catcatgaga aggtaaccat ccacgataga atgcagcatt 81000 agcataaaca gagcctgcat cattgtgaat aactactcct gatggattag ctcctcgacg 81060 accagcgatt cctttataaa aacgagtcat tatttatcac cttccataag tttatgattg 81120 gggaatgata gcaattgtcc attaacatct agtttagtaa aaatttcatt gcttcgaact 81180 actggaaatc ccttatctcc aaaagagaag aaatctcctt ttgaatatcc atcaaaatct 81240 ttcttttcac cttctgcggt catttctact acgccatttt cattatattc ttttaacttg 81300 tcaacagtgc ctttttcata tgggtccatt tcgatataat cttctttaga ctccccgact 81360 gtaaatacgc caattggaat aagtaaatct ttttctttaa acatatttta caccttcctt 81420 ttattttttg agaatttcct agctcaaata cgttaaaatc ctatagctaa aaagtacaaa 81480 aaaaagacac catttaaggt gtcttaattt attattaaat tgccatatca aatttcatct 81540 gatctcccac agaggaatag ccctttagta caaaatcgct tggttttata tcataaaaat 81600 tagttttatc aggaacgtcc aagtaaaact caatttcttt ttgttcctct tggttcatat 81660 tttcaaatct agcaactaat tcttgagctt gagcaaaata tctatcataa atatgcaagt 81720 tcattacatg ccaagaaaaa gttcctacct tccacccaaa atgtttagcc accatcattt 81780 gtaatgctac atattggatt tgattgatac ccatgccggc tatgagaaaa tctgaactgc 81840 gttgaattaa actcatatcc aaaaacattt ctccattaac ttctctaaca tcccaaatag 81900 tttgaaaagc acatggcatt aaaccttctg tttcatctaa gtctttatat tgccataaat 81960 taataatatt tctacgatta tatgggttct tttctagtcc ttctattaat ctatcaatta 82020 gattatagcg attaactgtt gctccataac gttgtccaat agttccatcc tctaaagccc 82080 aatcttccca ccaatggact cctctatctc ttaatggctg caaatcagat gtttgatctt 82140 gatatatcca aaaaagttcc tgtctagcta atccagtata tacaggacgc aatctactaa 82200 tgggcaactc tcccttgtct aaataaaatt tgataaatac atctgttata tatttactat 82260 tggcggtttt cccatcagag gcgtatttag gacgagctcc taaagaatta actccattat 82320 ctaggatttc agaaatacta gatgcaacaa agtgatccat ttttaatgta ttcaattatt 82380 cttcctcctt aaagtattgt tctaaaaaac tactaatttg atttgctgtt agctccatat 82440 tttcttcgtt taagtagtca gaaagctgaa ttccagattt tctctttttt gtgatataca 82500 attctttgtt gtgaatatat acatggatat tttcttccat aaatcgaaaa taaaatttgt 82560 tttccccata aagattaata tattttaatt cagtatcatc taaagaaata gatagattta 82620 aaaaatccca ttctgaagga tctttaccag catcttttat tgtctgttca attaatttta 82680 atacaggctg aacaatattt ttgtctacat aatcttctac taaaccttca tcataatcaa 82740 gaaacataaa aatctcttct cctattcttt ccctaaatta taactaaaat ctattgtatc 82800 aactttaagt gcgttagctt tccaaactaa atcttgaatt ttactagcaa ccccttttgt 82860 aatcccagga gcatccttac cttctaattg tttaacagga acatttttga ttgtaatccc 82920 attcattacc atatcttttg taattaatac gttaaacatt aatatacttc ctttcctaca 82980 ttgtaaaatg tttttactgt ttctaataat tgatttttta aaacattctc taatttttta 83040 tagtcaacct tatattgttt tttaaagtct ttggctgtca acatcaattc ttcaggagta 83100 agtttagcaa agtctgtata tctatgaaga ttttctctaa ccaatacttc tttagaggaa 83160 tctttatcaa agaaataatt tagagtaaat ttatcctcta aaacagcagt aaacatattt 83220 tctaacaata acattcttga aagttttttc cctgaatctt catttttaga ctctgataaa 83280 gagaccccag caagactaaa aagaaaatta tcttttctct cttctaaaac ttgtgttaat 83340 tgttcttctg taaataatct ttttcccaaa agaggaggat gaagtaaatg ggcgctattg 83400 aaggacaatt tatttagctc atcatattgg aatcgaacat ctcttacaga aacatgttct 83460 acattgtttt cattaatagc cacctcttta gaaagctttt cgttaaagca caaatctgaa 83520 aaggtggggt atacatagac cttaaaatca aaatcacttt caggagtaga aactccataa 83580 agagcgctac ctgtcaaata tacagaatga acttttctat ttaattttgt ttctactgtt 83640 tttactgtac tttttacacc ccttactaat aaatctttat tcattttaaa acctcctatt 83700 tttatttatt ctacttatag aatattattt tctgttggat attttaacta acagaatctt 83760 caacggcaga agttgctaac tcatctgcaa tttcattaaa aaaattatta ttatgacctt 83820 tttcccatat aaaataaact ttaagtcctg agaatcgagc gttagaaaca aaaagcttat 83880 attctgtagt taagggttta tttgtcttcc aaagattgtc agcccatttt cctaagcctt 83940 cataatcgtg atatatttca atttttgtat ctactttatc tataacattt tcaaggcacc 84000 aattaatagc ttctttagcg gcttgaattt ctccatcaat ctgtctagaa actggattgt 84060 ttacaatacc ggaagcagca tataaaactt cttgctcctc tgcttcaaca caaaagcccc 84120 aaccagctac gttaggatta actttttgtt tatagcttcc atctacatga atcttaataa 84180 tcttttttat acttttcgcc tcctaaagta agtctactga tacaattaat tctctatcta 84240 aatcttcttt caaatcaact aataggatgt tcaaaatttc ttctttcatt aagcaacttc 84300 ctttcttaat taacttacat atatattata taattttaaa taaaaaaatt caactaaagg 84360 aaatccaata gttgaatttt ataaaattaa atattatatt cttcaaataa ttcctcaaca 84420 tctatagcaa taaaatctaa attatcacaa atattattaa tcgtaaaaat agttaaggca 84480 tcttcttcaa accatggaac tccactttcg tattcataaa gtgcttctcc atctatatta 84540 agaatttcag ccatctcatc gatactaatt ccatgctctt ctctatattc tcttatttta 84600 tctgcattga ttctcatatt aattctcctc tctttattta tctctgctaa agataataaa 84660 aagggaaaat taataatgac aataaaaagc ttaggttatt ctggtaaaat ttctcctgtc 84720 tcagcatcat agtatagata accattttca tcataagcca aaatattaat atgatttgtg 84780 atcggcgcag ccccgtcaat aaaaatatga tccttatttc tataaattga atcttctttc 84840 tcttggggaa ctccttctgg atgcaactct ctcgtcatta tatgacctgc tacaatttct 84900 ttattccacg gcgtataacc aagagaagct gggtatttcc aagtcatttc ttctttagag 84960 gaatctgtcc aatgcccact tgactcttct tcaaaaccgg catgaacaaa caaagcattc 85020 ccatgttcat aaaaataatc taaattttga taccaatcta ataattcttt gtattctgtt 85080 ttaattgtat caacaacttc ttgtgtaact ttaatttgat tattaggatc acttatgtca 85140 taaatagcat ctacaccgtc aaaaattaag ttccactcct tgttaagagg ttttataaaa 85200 cttttgacag tagttaatgg cttatcatta attaaatgaa gagtattaga aggatttttt 85260 aaccactcca aaaacatttc atcatgattc cccattaaaa caataatatt ttcttttcca 85320 tgtttttgtt gtaagtcata aatagtcttt aaaacagaat atccatcagg agaacgatca 85380 atataatctc caagaaaaat aagtttatcc tcaccaatag aaatttttgt tttattaaat 85440 aagaaatcta aagctttttc taaggcttga aaatttccat ggatatctga catagcatat 85500 acagtcataa ttttgtcctc ctattttgtt atatatttat attaacattt tatacataaa 85560 aaatcaaaaa ttagggtctg ataaatattt attaatttct attgcggcat tcgcaccttc 85620 tcctacagca atagcaattt gtttattatt cccataaact acatccccaa tggcaaagat 85680 gcctaaatct tctaccttat tatttctcat cgtttctaca taaccatctc gtaaaatata 85740 atcaaagttt tcagttaaaa aatcagtttg aggaacttgt cctatagcta taaaaatacc 85800 atccacagat aaagttctgt gaccaattcc cttttcatta ataacaataa aatcaacgtc 85860 gtcttttccc ataatggaag taacttgtgt atccaagaga aaatggatat ttggagtatt 85920 ttttgcttgt tttacttcgg catttgttgc tctaaattct tgtcgtctat gaattaaata 85980 tacatttttt gcatgacgag ataggatttg tgcatcttct aaagcagttt gtccaccacc 86040 aacaattgct acttctttat ctttaaacaa aggagcatca caaatagcac aataagatac 86100 gccaaaacca gcgaaatctt cttctccttt aactccaagt tttctaggag aagctccagt 86160 tgcaactaat acagtattac cactaatttt ttctcccata tcggtagtaa ctataaaagt 86220 tgaatcatca attttattaa cattttcaac cataccaaaa atttgtgtaa tatgttctaa 86280 gtcttctaca tgagagtaca tatcttttac caagattggg gcataaattt tcccctttcc 86340 aagataatta tctacaactt ctgtattttg aatttgtccc ccaaaaccat aaccatagat 86400 ccatgcagtt tttaaaccaa gccgagataa ataaaggaca gcagacattg ctcctggtcc 86460 gccacctata actattgttt catacattaa tttgtcacct cttcataagt tttaataaaa 86520 atatctggtt tgcaaggata taattctcca ttgattcctt ttataatata atctccttca 86580 gaagctttca ttacaccttc taaagttttt atttctatcc aaatttttcc cccaccatat 86640 tgagaaaata aagtcatttt atctgaatac ttacgtaccc aactttctac tgtttctaaa 86700 ttttctctgt taaaaagcca tacctctacc tcaacaggtt ttttacgata tttttttaca 86760 gaactttttt ctgttttttt attaaaatta aacattaaaa tccctccatt aaatatattt 86820 tatcacattg aagagatttt ttcacatcaa tacatctttg attgtcagag ccatattttt 86880 taccttcgat tctacgatct atcataaaac gaccatcaat taatacgtct aagtattcta 86940 atactggttt aagtagttta cttttagtta cttgttccca agtaaaacca gtccaacaaa 87000 taacttctgt ctctggtctt tctgttttaa ctgccgataa aatctctaat gtatctttta 87060 tatttttagg actaagaggg tctcctccca aaagagcgag agtatttaat ttcattgggc 87120 cgccatcaag accatcaagg acctcttcta tcacctcttg gttatcctca tataagtttt 87180 catttctttc ccaagtttct gaattccagc atcctggaca atggtgtgaa caatgattaa 87240 accataaaga aactgaggct ccttttaagc cactgttttc catatcatat ttttttatat 87300 tgtgtaaata agccatatta tgctccccca attaaaatta ggataaacaa aattaagaat 87360 atggcaaaca ttaagtaagc catattctct aatctcgtag tatctcttcc tatatttgct 87420 ttcattccag aaccaattaa taataggata caaatcccac aaaaaactgc aataaacatc 87480 attttaaaaa acttccttcc tttttatttc tctttatttt taagaatttg ttctaataaa 87540 acttcatttt ttgaaatttc tttatccaaa agtttttcaa gcattaaatt tgcaaataaa 87600 ctaaatgcaa aataagataa gccagttaaa gcaacaacat taataaatgc agggcaagca 87660 aagattaaac agtatcctgc gataagaata aaaagtatta aagaaattgt ttgtactttc 87720 ttttctgcta aaacaacatc catttctgat acataagcat ataacttttc aataacctct 87780 tgttcctttt ctttcatata tttctccttt tatttgaaat tatgtttaac tctttcttta 87840 acttcttttt gtttaccagg attatagcgt gagtcaccat gaatctgatc aaaggaaaga 87900 tacccacaaa ctctcgttac tactgtaata tcatgagtac cacatttagg acagttatca 87960 tggaaatctc cactataacc acattggtta caagttcctg caacaacgtt aactccataa 88020 tacattcctt tttccatagc aaaattaatt gcttgttcta gtacagaatt atctactcca 88080 taaacaaatt cattataaga aattcgccca cctgttgcaa gtttatggaa tggtgcctca 88140 aaagcaattt tttcaggaac gctaacatct tcccatactg gaacatggaa agaatttgta 88200 atgtactctc tatcagttac atcttcaatt actccataat ctttacgatt aattttctgg 88260 aatctatagc ataaactttc tgccatttac accctcggtt tcccgatatt tattagggga 88320 ttggactata ttttcaccct ttttctcaaa ggggactgct ctttcgcttt cgctactcta 88380 ctctcctact aggatttcga tagtctctac accttacgaa ttttctattt ttagaaaata 88440 tacttgcaaa tattttctaa ttggttttct atttttttta acttggtcgc aaattgttct 88500 ttttggtata cctgtttctt tatgcgcatc tccactatta ataaaggttc gaataagtct 88560 atctgagtcg aaatcaaaca tccctatcaa ggactggttt tctagcgcat ctgcatcatc 88620 aggaaaacgg aaataaaact cttttctaca aggtctttta taacgagcct gtctagaaat 88680 tgtagtttta tctattcctg tttctcttga agcctcagaa atactaccaa aagaattaat 88740 caacttattg gttttggtac agaacattaa aactggaata ctttgactat cttctgcccc 88800 cttggcattt ttagccaaac catcatcaaa tgcttttttg gtattttctg atgtagtcgt 88860 ccaatacaag ttctcaaccc tgttatcaga tttaatattg tttttatgtc cgacaatatt 88920 gtagttatta gggtttggaa taaaagcttc agcgaccagt ctatgaactc ttttattcac 88980 tacccctttg ccaatataat ttattccaca atatttatag ccgtgcacag tgaattgagt 89040 ctttttgaac agatattttt tttggttgtt tcgaccattt aatgcataga tgtctccatt 89100 tatatcaacc caatggtctt tggaaccttt tatttttacg gctgttttag gtaatttcaa 89160 ataactctcc tttcttgcaa gtttttgtaa aaattcgtct tggcacggga ttagcatttt 89220 aaagcctccc ccgttagcag gattcctccc acacctcttt taagagttta gcagtttttt 89280 cataatctat tgctagatta gcgcccacat aatttaggcg ttgaataaag tgcatataaa 89340 tgattataat taattgtagc tttttctgtt aattttttca aatatgcaac aattttagtt 89400 ccatgtttaa gatattcttc ttttggaaca ttcattgctt ttaacgtttc ataaattcca 89460 atatatccta atgaagcagt tgaggcttca tatatttttc caacttcttc atcatagccc 89520 acttttgtcc aagctccacc ttcacagaaa tatagagggt tagaggagcc ttttgtttta 89580 ctaattttag tataataatc ttcgtgaata gcaaaaacaa tatcagaata tttatctatt 89640 agatcaaaaa atttattcca gtctcctttt gactctaagc caattttagg aagattcaac 89700 gtaacagctc caatattaga tcgtccagtg taaatttcat tcccttgttc atcccagaac 89760 gggctaagga aggccctaca acccatccca gagacaactt gattagatct ttcatatacc 89820 tctgcaagat ttccactatc tagtgataac caatcaggat atagtcttaa tcgagaacat 89880 tgaattccta attgtttaat gtcgtagtta ggagattctg gactgccatt aatttccttt 89940 ctatgtaaga aagatagttt aggaaataca gctgttgatt tattttctcc aattccttta 90000 attcgtacat tcaaaatagc tttagaaatt tctcttcccc attgagttgt atccaaacca 90060 aaagtaattg ttacaaaagg gatttgtcct aaagaattac taattgtatt taattttgtt 90120 tcaaagcctt gaaatccttg ttctaattct ctaacagtca tctcataggc tgtttgttct 90180 agtgttccca taatatcttc attagtaata ttcccaaatt tatctaaaat aaattttaaa 90240 tttgattgat aacttttttc agcatattta gctaaaacag tatctatttc aggaatagta 90300 aaaccaccat attgctgtgc agaagcaaat aaagtaacat ctcctactac attaatagca 90360 gaatcaaaat gtttaggctc tgcataaact gttccattta attggaaccc accatctaat 90420 aaacctgcca tgttaaacaa acaacaattt tgctggcgta aataacgctc tgccaagtca 90480 tgaatgtgta tccaaccttg tttatgagct tcaatccaat ctttatccat ttcaaatgtt 90540 tccatataac cacgcatatt attgtttgca attaaggctt gttttgtact attcaagtca 90600 ctgtctttat tagcattttc tttatctcca tcatgaagaa cagtttcaga ttcctcataa 90660 ctttttaaaa atgttttagc catttgtttt ctatagtttc tatacattcc ataagaagct 90720 ccaacaactt cccattcctt agacagaatc tcaagaacaa cattatgaat tttagatgtc 90780 ataacaactg cattttcatc ttcagttgaa tttaagaaac gagctacctc attcgcaata 90840 attaaagctt tttgctgcat atcatactct tcctcattag aaagaatttt ccttgcagaa 90900 gctaaaattg cttcctggat tttcttcgta tcaaaaactt caatatgacc atcttttttc 90960 attactttta acataaaact ttcatcctct cctatatcgg tagtttattt aaaactcaat 91020 ataagaaagt ttctctgaaa aaaccaagac attctatctc atttaatctt atatttttta 91080 ggcgacccac ctaaatcttc taagaaacct tcttctttta atgcttttaa tcttgaagga 91140 gtttgtctat tagtcaagtt taattcctcg cataatggag ataattgact atttttaaac 91200 tcttctccat tagggaacat tttagacaac aaatctttca ttgcatctaa ttcttctttt 91260 tgaatacctt ttttctttga agttttagga atctttttaa ccacaatttc tccttctaca 91320 ggtttattac ttttttctac tcgttcgtca taatcatata gttctaaagc tgcttctctt 91380 gaaatattta aggcactcat aaatttttta ataatttctt ctttcattta aaaatacact 91440 ccttcaaatt ttagttcttt atttatcttt ataattaatt ataacatata ataatcaaaa 91500 agtcaagaat ttttactctt agaattactc aaaactctat attaattata tatatttaac 91560 atgaaaaagt caacccaaga gagtgatagt ggggagcaga tttgtaatac atgaacaaaa 91620 aacttccttt ttaaataaaa aatatttata aatatatata ttaaacaaaa aaagtcaaat 91680 atgtttttaa ctagaggtta aactcttcgt tgctcttggt tgaaaaactt tttttaaaat 91740 tatataatag ttttgtaatc aagttttaga aacgaaattt caaataaagt ttagatttta 91800 aaaacattat ttgaaaagta atttttaaaa tgatataata tttatataag ttaaagaaag 91860 ggagtttttc atttatgcta aattataacg ttgaagaggt taaagcaaat ttaaaagaaa 91920 tctttaatga attttatgag tggggttcat tatcagattc acaatattta gattattatg 91980 caaaaggaat ggaaatgaaa aactcttacc tacaaaaaat ctttgatgga acaaatggaa 92040 gaattgtttt agatcctaaa gatttcaaag attttatgga agaattttca tttgaagaaa 92100 tgaaaaatta tgtagaagat gatttatatt ctgatttacg tgatttgaat atttcttata 92160 atttcattga cacttttaaa acatcagttt gtaatacttt tgacaaaatt agagttgaaa 92220 ataaaaaggt aattatgaaa gatgaattta aaatgttacc ttttcatgga caacgtttaa 92280 ctaaagtttt aaactcttta gttgacatgt cagcattaac tgctcaaaaa atttcttcaa 92340 ctaaaggagt aaaagaagct atttctgata ttaaaaataa aattgttggt ggagaaatgg 92400 ctagctttgc atcaggagca aattttgatg gacgaaaaat tgttctatct atcagaccag 92460 aagatttatt cttaattagt tatggtaata gttggcgttc atgcatgcat cctgatgaag 92520 gtgaatataa taatggtaca ctaggttata tggttggacc agacgctttt gttggtttta 92580 ctgtaatgga aaaagattta gaagaatttg gttatttagc tccaaaagta tggcgacaaa 92640 tgatgtttgt ttctgaagtt gaccatgaag aagaaaccgt tccagttctt ttgtcccaaa 92700 aaggatatcc tcgtttgaat aatgggttgc aaaatatgat tagtaatgca actttatctt 92760 tattgggttg ggaaggtaat gcgaaaatta accaaataga agaaaattta acccgctttc 92820 gttggagaaa tggttcagat tgtaatgaat atggctatgt agatgctttc aactggaatg 92880 atgcaattcg aggtttacga tctccggttc tttttcaagc tgaagaagct aaaactattc 92940 taattaatgc acaaagtact tttgtttgtc ttgaatgtgg ctctaaagat gatccctcaa 93000 attgctcagg actttgcgaa gaatgttatt atggttcacg ttcaatttgt ccatcgtgtg 93060 aagaacgttt tgatgaagac tatggggttc gagtattaga tacttctgga gatatcgttt 93120 atgtttgtga atactgtgga gaagaaacag tttggtctac tgaagaagag gaaaatattt 93180 ggagtgaaga agctgttcat gccattggat ctaaagaaga ttcttgggtt tatacaacaa 93240 aagctactta ctcagattat ttagaagaat atatcttgga ttctgatgct gcatggtgcg 93300 aaactcttga cagttatatt cttcaagaag atgttgtaga attgccagat ggaacaactg 93360 cttgtatcca tgattgcttt atttggagag ttgatggaaa atatcatgaa gatacagaaa 93420 atattgattc tgatgaatgg aaagaaatct tttctcagga actaccaaaa attctcgacg 93480 aagcttggaa aatgaatgaa cttttcaaaa agtatcaagg attaggtata tctaaaaaag 93540 attgttttga aattttaaag gattatattt ttaaatgtga aaatcgggag gaagaacttc 93600 aattaaaaac taaaattatt ttagattctt tagatttttc atgggaacct gttgaagata 93660 gttcctttga atttcgtttg actggagaca accctgttaa taaagaattt attgatttct 93720 tattaactgg cgaagtaaaa gaataatatg ataaaagccc tattttataa tagggcttta 93780 ttttattgtt tcaaattagg tctttttaat aatattagcg attaccaaaa ggaggaaaga 93840 gaatgaaaaa agatatttat gtatgttctc attgcggacg agaatatgaa gaaaattgta 93900 tggttatcaa tcatcgtaaa aacaagatgg aagatttcta ttatatgatt tgtcctactg 93960 atagagctat tgcttataaa aagggagagg tttcaggtga agagcctatt gtagggaaag 94020 ccatagtagg taaggcaaaa gttggaaaag cagaataaga ctttttcata ataaaaagag 94080 ataagttctc aaatatattt aaaaaatagg aggaaacaaa atgagcgcag aacaaattaa 94140 agcattattt gcaactggta aaactatgac acaagaatca tttgcttctt tagtagattt 94200 cttgcaagaa caaaaagggc ctaaaggaga cccaggcgaa actggaccta tcggacctgc 94260 tggacctaaa ggagaccaag gtgaggctgg aactcctggt gttgccggac ctaagggaga 94320 taagggtgac caaggagatc aaggagtccc tggagcaaat ggtgcagatg gaaaatctat 94380 taaagctatc gtattaagaa caaacgaagg cggtcaagta actggaggaa cagctactct 94440 ttctgatgaa tctacaattg aaattacagt tagccaagct ggtgctggtg tataaactat 94500 ttaaaattaa tcccaatcta gatgattggg attttttatt tgaattttta tatattgaat 94560 gatataatta attataagtt aaatgaaaga aataaaaaga taaaatagct tatgagaaac 94620 aacaatttga aaaagttagt tcaatttgtt ataattatat tatagaaaat gaattaataa 94680 ataaaaaata ggagcggatt ttaaatggaa gaaattaaat taacagaaaa aggtacgcaa 94740 gttctaaaag ctttacaaga ggctcatatt gataaagaag aagatgcagt attttctaaa 94800 cgtgtagcag aattagcatt tatgatgcct aatggcgttc caggtgtatt aacttcattg 94860 aataaaaaag gactagtaga aaaaactgct gacactcctc gaaaatattt tttaacagag 94920 caaggagtaa attttaattt acaataataa aataaaaaag ggagattttg caatggaaaa 94980 aacatatact atcgaagaaa aattaaaatt gatggaacca atgatgaaaa aatcttggga 95040 agaaattgat aatattttat atagcatcaa tggtggtttc gttacggata ctagacaagc 95100 atgggcagaa gattgggctc aagcaaaatt tcctattttt cagaaatttg gaaacaaact 95160 aagactagaa ggaagcgttg aaaacgcatt gtcaaattca gaaattaaag atgcttttta 95220 ttcttcattt ttatctaaaa ttaaatcaat gactatggaa gaaaatgaag ggaaaattat 95280 agcactttat tttagtagtt tttctttaga agagttacaa aaaaaccgtt ttacgacaaa 95340 taaagaaatt ttaggcgaat cattccaagc tggagcaaaa atttctaaat ctcttaagcg 95400 tgttctaaaa aataaaaaac tagttaacca aatacagatt tatctatcag cttttaatga 95460 atcattattg gctcgtgggg ttttagaagt ttctattgac cctcttgaca ttttaatgat 95520 gtctgtaaac aaaaccagag aatggtcttc ttgtcattct attattgatg gttgttatgg 95580 tgctggacca gtttcttatt tattagacgg ttcaagtttt atttgtcaaa tcattttagg 95640 aagtcaagat agtatcccag ataaaatttg gcgtagaatg ggtatgttca atacagagtt 95700 agatgctatc cttttatctc gctcatatcc aggagttaat aaaaataata ctaaagctct 95760 acgtaaatta tttgtagata cttttggaga agataatgtt gcttatggat ttattgattc 95820 tcctgatgca gaaaatacta ttagagaaag tagtgaaatt cactataatg atattacaca 95880 tggagctatg aataaagttc caatgattgt tttagataaa caaaaataca atgttgaagg 95940 agaaaataat tctgaaaaca ttttaagaaa acatttcaat aaggattttt atgatgaaac 96000 tgatgaaact gaattatgca atgttcgttt tgaagtaggt gttgattcag tcataagccc 96060 aacttgtgat ttcactatta atggatcaat tgaagctgga ggcctctttg acgaagatgc 96120 ttactatgat gaagatgaat attgggacga cgaagatgat tgggattatt aaaaaagggg 96180 gaattttata aaatgcaaaa gaaagaatta ggtttacaaa cctttaaaaa cattttacaa 96240 atgacacaag aaggacttca tttttattta ttaggtcaaa tagaatcaat gtatgattct 96300 agagatattt atcgtgttga tggtagtttt atttatgcta aaggagatat tcctgttctt 96360 atgttggctc atttggatac agtccataca gtagctccaa cagaggaaac tatttttcac 96420 gataaagaaa aaaatgtttt atggtgtccc gatggtattg gtggggatga tcgttgtgga 96480 gtttttagca ttttagatat gttaacacaa ggttatcgtc ctcatgtatt attttgttgg 96540 gatgaggaaa ttggaactat tggatctagc ttctttacaa aaaatgtaga aaattatttt 96600 ggagaagatg tacaaacagc cttgacagag attaatttcg ctattcaatt ggatcgaaaa 96660 ggctttggag aatctgtcta ttattattta gataatatag attttgaaga atatattaac 96720 agctttggat ttaaaaccca caatggatct tatacagata tttgtcaagt ttgtcctgaa 96780 tttggttttg caggagtaaa tttatcagcg ggatatatgg atgaacatac atgttatgaa 96840 cgcatttata ttaacgaaat gtataagact cagagaaaag taaaggaaat tttagaagac 96900 caatatcaaa atcctaaatt ttatgagtat aaagaagatt cttatactgg atataataat 96960 tatggatatt ctaactatta ccaaaatggt tcttattgtt actggggaga tgaagatgtt 97020 tatgatgaat cggattatct aaattatgaa gatgatgaaa caagttttgt tccctataaa 97080 agtcgcaata aaaaaaaaga aactgaacaa tgtgaatttt gctttttaga caaagggaca 97140 gttccttggt ctgatacaga taatgaagta ttgtcgcatt tgtgcgatga atgtcgagaa 97200 aaatacttgg aagatgtatc acaaaaagaa cgttatacca aagctataaa taatgacgga 97260 aagtaagatg gtgaaaattt gacagaaaat atgaaaagtc agtacgaaaa gattaacctc 97320 tctcaagggg ttagtctctt ttcgtataat aattatgaaa attttttcag tcaagttact 97380 gaaaagaaaa aagaaataat tgctatgatg gataataatc taagattagt ttttaaaata 97440 gataacataa aggctataaa aacacaagca gaaagagatt ttaaaaaaga gtttaaggaa 97500 cgatataaag aaaccaggaa aatagacgct ggcttcttta aattagcagg gctattaaat 97560 gattcagatt taatagagaa taaacattct tctggcaaaa aattatctaa ggttattaat 97620 caaactccag aactaggaga agaaatcgta ttaatgtatt ctcaaataaa gaactcatat 97680 atacttccat ccagttattt ggttatatct gttcatcctt atgatttcat aatgatgggt 97740 gttggcagag gatggactac atgttataaa ccaatgggag aacattatac tggaggatat 97800 tctgcagcgt tagataatta tacattttta acttatgtta tgacagaaaa acccaacttt 97860 aatgatgttg aatatgtaga tagaaaaatt tatagacgtc taggggtttt ttcaaaagac 97920 tataagggag caatgttatc tacacaatat ccatataaaa ataatggaat tgaagaattt 97980 acaataaata ctttacaaag cacctttttc tcttctcttg gagagaattt tatctcagtt 98040 catgacgata atataaagac ttataaaaca aatttaagtc aagtatataa tgattttact 98100 ttaggtataa aatcaaaaag agagaattta tatataggag cgccaaaaga atctgagata 98160 ttaaaatatg gatctgttgt aaaatgttta tcttgtaata aaacaaacgc tttttctgat 98220 atgcctatat gtggagattg tgagttaaaa acctatgaag gaaattggag tgataaaatt 98280 ggacaatata cagtatgaga aatttttctt tgaattaatt gttgatgaaa aagttggtat 98340 ccaatattta gaagaagagg gcgaagcaaa gcttgccttt actatggaaa aggatactga 98400 tgaatatttc aaggataatc cagagatttt tgataaattt attgattatg tacaacagtg 98460 tttagttgta gcaaattatg ctggttttga ggcaccatca gaattctata cagctattaa 98520 agcctatgta gaatctgaag gagatggaat tttatgatgt tagattttac tggacagcct 98580 gctatctttg gtagcgggcc tttggtctat acagaatatt ttaatcaata tttagaaaat 98640 cgttcaatgg aagatattag attttttgtg tctgataaac ctatcggacc tattctaaat 98700 tcatttataa actatagcat aatagaatat actgcaaaag ataatgaagc ttgttctata 98760 aaaactaaag aagatttata tctagtcttt tatagtatat atggcaatat tcttccaaga 98820 gaagcaggaa ataaggtggc gagagattct attatttcct tcatggaatt gttagaagac 98880 ccagacatgt atttactagc agagaaagat gtattatggt ttggtaaagt tgccaataag 98940 tattttgatt atacttatga tggtacttca gtctttatta aaaacatttc tggattggaa 99000 taccatttgt cagaaaatta taatctttta gtaaatgagc caaaagattc aaatctaacc 99060 gaaataaatt tcatttataa tagattaaat tctatttatg tagatcctgg taaattagct 99120 atgttagtag taaattatgt gaaaggttct tcacaccaaa ttaaattatt ttaagaaact 99180 agatagatga aggaggacga gcttgttttt cgcatgttaa agaattttat agaaaaacac 99240 aggattcctg attggttagg agggttaaaa gtggagctat tcaatagtag agttttagtt 99300 cggcatcaaa tagagctaga aaaagctcgg aatgcaattg aaaaaagaga tttattaatt 99360 gaacgctgga attttactga gcaatcaatt atacttccag ccaaaaggtg ggagcaagaa 99420 gatagagtag tttctatttt tactttccgt tcttttctta taattttgct ttcaatgctt 99480 ttagtttttc ctgctatatt tatattgcct tatatggtaa ccaaatatac aaaaagggaa 99540 cgtttaaaaa aagagatcat gaaacagcaa actattgtaa gaagtacatt gccaattcct 99600 gtaaatcaag atgagtataa agaaatagaa gatacttatt ggggacagaa atttatttcc 99660 aatggtatta tttttctaaa aaagtcagaa agtttttgaa ccctttaatc ttttagggtt 99720 ctttttttat attaggttta aaagcttttt aatatacata agagaaagat tataaaggag 99780 gacaaataaa tgtcaattca tattactaga ggaaatatgt tatacggcac aggtagacca 99840 actattagtg ctgaatatta tggacaaact tatattgata caggttcaaa tcctagagct 99900 atttgggtag ctacaggaca aaataatgaa tcatggaatg aggtttctca aaaaggtcac 99960 aaacatacag tatcagattt ttctgatttg gtaccagaga ttacaaaaat tgttcaagat 100020 caaggtatag aggcagcctt aatagctctt aagggtggaa acaatatatg ggaaggagat 100080 tggaacaatt ttactggagc attacgagta ggaggagcat cagtattatc aaccaactct 100140 tctattggag aacttaaaga tgtcacgctg ggagaaaatt taaaagttaa tacattgctt 100200 ggttggaatg gtagtacctt tgttccttat gaagtgactg gtgcatctcc aagcgaaggt 100260 ggaattgatt tcagtcaata tgttcgtaag gatgaactag tttctacatt agatagttca 100320 gcttctgata gagcattggc agcatctgct ggtaaaactt tgttagaaac aatgaaagca 100380 agatattcac ctattgatca cactcatacc aatttagctc cagtaaatca tagacatgaa 100440 aattatttag ataaaactat aaatcaaact atgacaggaa gtcttgtatt agatacaaac 100500 cgttcaggac tcccattaag tattagaaat agttttggaa gattagattt tcaatttcca 100560 ataagtccaa cagattcgtt agttatagcg gcaagtggta atggtggaac agaattaaat 100620 gatattattt ttggtggtgt cgatgggaat actggtggtt ctttgacatt gaattttgga 100680 cgagttcata ttcctgcggg tcaaatgact attggagata ctaaacggta tttaggtatg 100740 ccagcactaa aaatttctga acctaattta acttatgtag gtaatttaaa tcgtaaacca 100800 gccattgata ttaatggtgg agcaatgaat ggtattggac agcttgtttt tagaaattat 100860 tctagtagtc gtgatgcagc tattttattc cctagaaatt acgccggtag tggacaacca 100920 acagaaagcg gatattatca ctatttaaga atgagcgatg gtgaattatt aacagatact 100980 gcattatctt ctgaacaatc atatatatca tttaaagggg ttagaatttt ctttagtgtt 101040 actgatccag gtaaagcagg tagagaagga gatatttgga ttaaaattta atttacttga 101100 cagggagaga gtaatatgtt aatatataga aaagatagtg atggtagatg gattcccatt 101160 gaagctcaaa ttttaaaaaa agctaaaagt gaacaatggt atccattaac tagacaaaaa 101220 gcacgaaaaa aaataaaagg tgtttggcga gacaattatt gacttttttt attaaagatg 101280 atataattaa ttataaagtt aaataaggag gaaattttag tggaagttaa aaatgtatta 101340 gaacgaatta taacatctga ttcttttcca gaatttgtag aagtagatgg tattaatgtc 101400 gtatctaaag tgactgttat tactacagca gaactgattg atggtaatat agtcaaaggc 101460 accggagtta gtcaaactca tccagatgat ttagatatgg caacagaaaa agtaggtact 101520 cgtattgctt tattacgttc ttatattgat atcctaaata acttagggga tctcaaaatg 101580 gatcatgaaa cttttgaaac cttagaacaa atgaagtctg aattagaaaa tgaaatccaa 101640 gcattcattg accgaaaaac agctatgtat gaacgtatta gagcaaatcg tactggagat 101700 atgactaata aagttcgaca ttttgcaatt tcagaagatg gaaaaacatc agtggaggta 101760 acacaataat ggaaaataaa acaggtcaac atccaacggt tgaacagcta ttagaacaag 101820 cagaatttga attagctgtt gctcgacaac aattaactgg aacacaagaa ttacttactc 101880 aagcaatggg tgaaatagca agacaagaag ttttattggc taaatcagaa gctgcaaata 101940 aacaattgct tgaagctatt caacaactac aatctccccc aacaattgaa gaaaaagaga 102000 ttgaagaaaa ggttgaagaa taatttctat taaaaaagac tgagttttcc tcagcctttt 102060 ttaatttaat aatttgaaaa atttatttaa aaatgataat atatatatat atagttaaga 102120 aataaaagaa ttttaaaagg agtttttatt ttatggaaaa caaatacggt gcattccaac 102180 aaactacaga ggatagaaaa tttatgtcta tgtattataa tactattatt acaggtagaa 102240 aatttaaaga aaatttgaaa aacggcataa agaaatcaga tcctaagcat caagaatttg 102300 aatatgaaat tatgaaaaat tgggacaatt atttaatcga agcaggtcgt gctactccaa 102360 caaaacaaat gactgcagac aaatggggat tagcgaatat ggcgttcggt tttctccaag 102420 aggacgaaga tattgaattt aaacttttgg attctcaaaa acgtattgct ggtattaaag 102480 atggcgacac attttatgaa gtaaaagtaa ctcgacataa aagtcctcaa gttgcagaag 102540 gagaagttgt tgtggctaaa aaactaaatc cttttgtaga aaaattagca gaaaaacttg 102600 aagaacatta ccctgttgtt gtagttaaaa ataatcaaat tactgtagaa gatgataata 102660 aagttcatac ttcagtaaaa attacaaaga aaaaatcacg gttattttaa tgtaatttaa 102720 ctgtaataat tttgtaatag ggtctttttt attaaaacaa gaaaagggga atttcatatg 102780 gaatacatcg ttgaagcaac aaaaactatt tttggtaaag aggcgtataa agttgtaaat 102840 aagaaattgg aatcattaga tgattgggtt tacgaggctt ttctattaga cgttcctttt 102900 tcagaagatg gggatatcgt ctttccattt ttcgaccttg tgaaagacaa ctttaattca 102960 gcacaatttg aacgagaagc tttagtagat tctcaacgaa attgttttac tggcttaagt 103020 gttgccaaaa ttaagttttt agaatttaat gaagcaaatg gatatgcttc attgcttaac 103080 gaaaaactaa aagaaaaagg agaataacaa tttgaagaaa attattatcg caggactatt 103140 agcaggagct ttatttattg gtggaacagg tgttcacgca gcagaaattc aaaaagatgg 103200 acagacttat tacgaagtag aagcaggaga tactttatct gctatcggta ataaatatgg 103260 attagatcat agtttgatcc atcaatctaa ccttgataca gttagtgatg ctaatatgat 103320 ttttgctgga gatttattat tgattccaac caatggagag gttcaggctc cagcaacagc 103380 agcggtagtt gaagctccag ttgtgcaaga aaacgttgta gaacaagctc cagtagtaga 103440 acaatcacct attgtcgaac aagctccttc aacaggaaca aattcagcaa aagaatggat 103500 tgctcaacgt gaatcaggtg gctcatactc agcaacgaat ggtcgttaca tcggacgtta 103560 tcaactagat tcagcttatc taaatggaga tcattctcca gcaaaccaag aacgtgttgc 103620 agaaaattat gtagctcaac gttatggttc atgggaagcg gcacaagctt tttggatggc 103680 taatggctgg tactaaaatt aaataatttg acaaaaaatc cttactatgt tatactttat 103740 atagtaagga tttttatttt ataatgattt taaaaggaga aaaatatgag accattagaa 103800 gaggtaagtg caattgtaaa tgaaattagc cgctctaaag ttttaagggt agagggcaaa 103860 atatattctc caaaaggaag aatagaagta agtcctataa taactaaaga ttctttaaat 103920 ttaatgtttt atgatcgtca ctcttatgag agagatttta tttcatccat gagcttatat 103980 tttgataaag aaacaacagg aacaaatatg agaactatag ttttttatat catagatgct 104040 ttaggatata atgaagatga aatatacatt aaacatgatg aggatggaaa atctagtggg 104100 tttattttat attctaccac agggaaatat acagctatga ttgtagatat agacaaaaaa 104160 gaggtttctg taataggacg agaaggaaaa gctgaatcag tatttatcga ttgtaataat 104220 ggattgatag atgtcttact tgccttgatt aaatatgctt ctatataagg tggtttattc 104280 cgtataaata tctatatatt gggttttata aagattacag aattaaaaaa ggagagattt 104340 atttgacagt agtagtaaaa gataatggat tgagaaaatt ggaatttgat ccacaacgat 104400 taattaataa attagatagc tttaaagaag gattggacgt tcatccagat acatttgaag 104460 catataagaa gggtgttatt aaacaaatcc agggtagaca agagattgat ttccgagata 104520 tcaatgatgt tattattcaa aatgcaattg atagaatcat ggactttaaa gatggagaaa 104580 tgatggactt tgataaatta ggcaatacac aatttgaatt tgtagcagct agggctctat 104640 taaattcgtt gtataaacga gcttcaaaaa atcgaagtta tgatgttgat tcaaaatatg 104700 gtgattattt tggtcttctt tcttctcttg gtgaacaagg tcttattgaa ccagaaattt 104760 ttgtaaaata tactaaagaa gaaattcaag aattaggtaa atatattaag ccagaacgag 104820 acttactttt gacttacgct ggtttataca atatgtctga aagatatctt attcgagcta 104880 aagataaagg acgttcagta tttgaattac cacaagaacg ttatatgaca attgcgattt 104940 ctttattaag agaagaaaca caagatagac tacaacatgt aaaagaatta tatgatgtac 105000 tatctaaaca agaagttact atggctaccc caacatttgc aaatgctgga cgaccagatg 105060 cacaattttc tagttgcttt attttaacaa cagatgatag cttacaaggg atctatgatg 105120 acaatacaga tgctgctcgc ctaagcaagg ctgggggagg aatcggtaaa ctttaaaatg 105180 ccgatttaaa actttgtgaa cctaaaatag ggtgtgtcct taacaggatg ctaacggtga 105240 aacctaagtc agaaatgata tggcaatacc gtgccaagac ttatatctat atcctttgtt 105300 aaaaaggatg tgataaaaat agaatatata taccagatag agaatttggt tacgggcata 105360 aaatattatg gacgaactaa taatcctcaa agacgaaaaa caacgcactt tcgagaattg 105420 gaacagaaca aacacataaa ttctaaactg caaaatgctt ttaataaata cgggaaagaa 105480 aactttgttt ttactatttt agaagaaata gaagataaag aaaaagtttt tgaacgagaa 105540 gaatattact tgaatctcga ggaggaaaaa tataacattt ctaaaagctc gaggtcccct 105600 ggaaactata ttcattatgg ttctgataat ggcttttatg gtaagcatca ttcagaagaa 105660 actaaattaa agattggctc aaaagcttct attagataca aaggggaaag caaccccttc 105720 tatggtaaaa agcatataga agaaaccaaa cgcttgttgg ctgagaaagc tagggagacg 105780 tttaagggag ttccaaaaac agaagagtgg aagaaaaaaa tgagagcttg cagtcctaaa 105840 aatattccaa ttttaataga aggcaaggaa tatattagca taaatgaggc tgcgagagaa 105900 ttaaaaattg atagaaaaac tatctcttat agactcaatt ctaaaagtga aaaattttct 105960 ggatatagat ataagtaagg tgtaacgact aaatgtttgg atagcttgga atcaagggtt 106020 ccaaacgaaa accaaacgag accttttggg tgagataccc aattgggtag cgcaaagcat 106080 atatctaaca aatatatgaa gatatagtct atgccactag aaatagtgga ataacatggt 106140 ttatgtgggt aaaattcgtg ctgctggttc agatattaga ggaaatgttg gtgctgcagg 106200 aggtattgtt ggttggctta aacaattaaa taatactgct gtttcagtag accaattagg 106260 agttcgtagt ggtgctattg cagcatactt agatgtttgg cattatgata tagaagattt 106320 cttagagttg cgtttaaata caggagacct atctaaacga gcgcatgaac tatttttagg 106380 tgcttctatt cctgatttat ttatgcaaca agtagaaaaa cgaggagact ggtatttatt 106440 tgaccctcac gaagtgaaaa aagtattagg tttttctctg gaagatttct acgataaaga 106500 aaaatgggat ggaaaatcac cgctagatcc tgatcgtcac gctttctctt atcactattt 106560 taaggctgta gataataacg atttacattt aaagaaacgt ttgccagcta ttgaaattat 106620 gaaaaaaatt atgcgttctc aactagaaac tggtttgcct tatatgtttt atcgagatac 106680 tgcaaataga gataatccta acaatcatgc aggaatggta tactgctcta atttgtgttg 106740 ataaactagc acctttaagg agcaatcctt atcgaaaaag ctatctaaac agggaaactc 106800 tttaagaaaa gacaatcctg tgctaaatgt ataaagtcta ccgaggaggt agatattgtg 106860 agatatggtt ttatatatat tacaataaac aaaataaata atatgaaata tataggaaaa 106920 tgtgtttatg gaagaaaaaa taattgggag aaatatttag gaagtggggt ttatctaaaa 106980 agagccattt ctaaatatgg aaaggaaaac ttctttaaga ttattattga tgaatgtgat 107040 tctgaagaag aacttcgaga agtagaagaa tattatatta tgcaattcga cgctgtaaaa 107100 agtaaagaat tttataatat aaaagcaacc tcaattggag gagacacttt tacgaacaac 107160 ccgaataaag aatatacaag gaaattgaaa tctattaatt cttctggttg tcgaaaccca 107220 atgtatggta aacctaaaac acagaaaatg attgacagca ttaaaaaagc aaatagtaag 107280 ccaattttaa ttaatgggat tgaatatcct tcagtaaatg aagcttctag gattctaggt 107340 attggaaata cgacaataca ttatagaata aactcaaaaa actttcctaa ttatattaga 107400 ctttatacta aatgccaaac gactatcgaa accgagcacg agttgtggga gtagagtagg 107460 gccaagtggc taggcttccg agtaataatc gggtaagtcc tttaaacgaa acggtagccc 107520 ccttttaaaa aagggtgatg atatagtctg ttctatatgg taacatatag cagttcataa 107580 gagaacgggt tgaaattaac gacttcaatc gaacatataa gagcgaaatt gtacaaaatc 107640 aatctcctac aattatgact aaagaaacta ttaatgaatt aggggaaatt attgttcata 107700 aacaaagtgg agattttgtg acatgtaatt tatcttctat tgttcttaac aatgttttga 107760 aagattttac tctttctatt gaaggaccgc aaactagcga cgatgttaaa gcatttgaaa 107820 aattaagaca ggttttacgt attcaagttc gtgctactga tgcagttatt actgttaata 107880 atttacctgt tttacaagcc caatatacaa ataatcgtta tcgtgctatt ggaattggtg 107940 aacaaggtat tgcagcagtt ttggctaaac aaggaatcca ttttgattca gttgaagcaa 108000 ctaaattaat tgctcggtta gaagaaagaa tcatgctaaa tattattgag gcgtcggcag 108060 atttagccca agaaaaagga agttatcctt tgtttgaagg aagtcaatgg aacacaggag 108120 aatggttaaa tcatcgagga gtttgtcaag atgatattga ggatgcttat cgagcagagg 108180 tttattccaa agctaagaaa ggtatgcgga atggatattt aagagcagtt gctccaacag 108240 gttctacttc tttattagct ggttctactg cagcggcaga cacagtttat gacactatct 108300 tttttgatgg taagaaagat tctcgtacac ctgtcattgc tccagaacta aatttagaaa 108360 catggttcta ttataaacca actatgttaa tggagtttga aggagagaga gatttgggtc 108420 atatgtgggc tattctccac aacgcagaac gtcaaaaatg ggtagatcaa tctacttcat 108480 ttaatttata tatcttggat gatattaagg ttaaaaactt acttcgtctt catatggaag 108540 tttggtctag aggaattaaa tcttcttatt atactcgttc tcatgacgct tctcgtgtag 108600 atgattgtgt tgcttgcagc tcttaaggag aaaagaatga aagaattatg gttacctgtc 108660 aagggatggg aggtctatga gatctcctcc tatggcagat taaaaagagg aaatagaata 108720 ttgtctggcg gaattaatag ccatggctat agacaacata ctctctatat ggatggaaag 108780 aagcaacaat tttatgctca tagattagta gcaatagctt ttttagaaaa tccagaaaat 108840 aaatatagtg tgaaccataa agatggaaat aaacttaata atgttgtttc taacctagaa 108900 tgggcaacta tatctgagaa tactcaacat gcttataata atggactatc aaaagcaaat 108960 tctgaaaagg gatctagaat ttctgctgac aaaagaagta taaaattaaa agcaacccat 109020 aaaaaaacag gagaagtttt attttttaaa aatagtaaag aagctgcaga ttttttaaaa 109080 aaaggtagtg gtaatattag aagtgctgca actggaaaaa ttaaatctgc ctatggatat 109140 tattgggaac aagtttgaaa taaggaattt cttatttgaa attccttatt tattatgata 109200 taataaatat ataaggttaa agaaaagagg aaaataaatg acacatgaat tatttgtaaa 109260 agcagccaaa aaattggtct tagattattc taacgaaaac ttagagggag actatacttt 109320 agaagatgta tatattgtct ggagtacaaa ggtactacaa aatagtaaat tacttttgtc 109380 tacagcaatt tctgatggga tgtattatga agtaacctta aatggagaca aggaagaaat 109440 ttatttagat gcttacaaga aacaaaagaa cattcatttt aatgtagagg atttgtaaaa 109500 aatgaaagca aaatttacag aagatggtaa ggtcttatat caaaacttat atgaccttac 109560 tgaggaagct gaaagtaaag gatataaaac tgaagatgaa gttattagct atcttcatga 109620 ttgttttgtt gttgatgaaa atcgaaaaac aaatgatttt tctgaatggt tagattcaat 109680 tgatgaatgg acaaatcgtt cagagtagta taaaggagaa gattttaatg ttagaaaaac 109740 atattagatt atttaataaa gatgcagaaa atacaaatga gtcaattttt ggagaacatt 109800 ctggattatt atattgggac gaacaaaatc ctgtatggta tgacgtatat tcagaactaa 109860 aacaaaattt ttggattaaa taggtagtcc aagtaaaact ctattaaatc ggtggaactc 109920 cttatgggac aataccgaga tagctaaagg tgtttccatt aaaatatgat aaaagaaata 109980 aaaggaataa aaaatggttt tggtcacaca tatgctgtgt cagacaatgg gaaaatttat 110040 aatcaaacaa caggtaaaga attaaaagga agaccacaga aaaagggtgg atacttaaga 110100 gttaatttag ctggaaaaga ttataaggtt catagacttg tcgcagaagc ttttgttcca 110160 aacccagaaa ataaagaaca ggttaatcat ataaatggaa aaacaaatga taatagggct 110220 gaaaacttag aatgggtatc tccatcagaa aatatttatc atgcctatac aaatggactt 110280 atgacaaata ttggggagaa aaatagaaat tctaagataa cagaagccca agcattagaa 110340 attaaaaaat caagtgaacc aaatagtgta ctaggtgaaa gatatggtct atcccctagt 110400 tatatagcta gaattaaagg gggttatact tggaaacacc tttagcattg taacgactat 110460 cgaaagggta gcttattgtc gagaggataa ttagcgagaa ccgagtagag tagggccaag 110520 cggtaggtag tgtatgcgaa aatggcatac atgataaatc ctttaaatcg aaatttagag 110580 acttagaggg taacgcctct atttaagata tagtctagtc tatatggtaa catatagaag 110640 ttcataagag aactgcataa aattagcgac tttatgtgaa tataaacgac ctcaagaagt 110700 agggttaaat actgatagta gagattggcg agataatatg agcgacattg aaaaaacttt 110760 ttacaaacga gctatttctc aattagtatt actagattct atttctactg ttattgatgg 110820 tcaattggca gcgtatatta aaaaccctgc tatcaaagct attatggctt atattgcttc 110880 tcaagaatca attcataatg aaagttatac ttatattgct accactttta tgacgaaaga 110940 agaagcgaag gaagtatttg aaatccctaa aacagacaaa tatattcttg gttctagtga 111000 aatgattttg gatacatttg aaacattttt ggaaactcca acaaaactaa atatggctaa 111060 agccttagca gctatggcag cacttgaagg tattagattt acaaatggat ttactccatt 111120 ttacttatta aaccgaaaca aaaaaatgtt gggaacagga caaattattc agttgatcca 111180 gcgtgatgaa gtacaacata gttatttcca aacgttagtt gtacgacaaa ttatgtctga 111240 attacatctt tcaccccaag agagtgaaga ttttagtaat tgggtttatg cgttctttga 111300 taaagtagtt aaatctgaac aggaattagc tcgagatcta tataaggatt atgctgtttt 111360 agatatggaa gaattagaac actatattga gtggcgtgct aatattctgt tgcaaaactt 111420 gggcttaacc aaagtttata ctgaagcttt aactaatcca atgatttgga ttaagactta 111480 cgattcagat aatatcaata atacaaaaac agatttcttt gaaaatcgtg tagtaaatta 111540 ttctaaagca acagacgaca aaaatagctg ggacgacctt taatatggat aataacgaaa 111600 aaacattttg cacgggtgga gtaaaattag ctccgcccaa ggcaattccc tctcatgtag 111660 aatctctctc tcttggacct aaagtgcttg tagtttatga gagtctatct gggaatacaa 111720 aaacttttgt tgattttata gtaaaggctt acccaaatgt taattttatt ataaaaaata 111780 caaaagatga cttgacaaat ttggaagaat atgataaaat aattatagga acttacacat 111840 ggagtaatgg aaagattcct aaaaatatta aatcttttgt aatttctaat agagatgtat 111900 taataaacaa accagtttta ttatttggta gtggaattac aatttatact catttctgcg 111960 gagcattaga taatattcaa attattttag agaaagatat ccctaagatt aagtttgaat 112020 taacttttat tccagaagag cataaagaag atttagaaat gtttaataat tttattaagg 112080 agtagtgaga taatttgatt tataaactat caaaagataa ttgtccatat tgtaatcaat 112140 tggaaggtta tttaaaaact aaaaatatgc aaaaatataa caagcatatt gtaagtgttc 112200 ataaagaaaa agatccagag aaatataatg aattagtaga aaagggtaat gtattatcat 112260 tgccagtatt aatgcgagaa gatggaacag ttttaacaac tggttttaat attcaacaaa 112320 ttaatttaat gttaaaacga gaatttggtg agtaatatga ttagatacta ttctaggaaa 112380 tatttttgtc cagtatgtaa gcaggaaaag gataaattaa ttcgtttata ttctgaaaaa 112440 aatgaacatg gagaaaaaac tttaaattgt aaatatcatg gttttcaaca atattgtaat 112500 tgtagtccta gtgaactaga attagttatt gaggacgacg aataataatg gatgatttct 112560 cctttttaaa ttataaagac aagaaaaagg aggaatcttt tattatgaaa gtaaatttaa 112620 atcttgacaa tttttgtcca atttgttatg atgaagaaag agttatgaga aatgtgtatc 112680 gcaaattaga cgataaatca ggagatctat attgtccaaa acatggttta gtcatgagtg 112740 aagatattga tttaactaga tatgttgaac gaacaattaa tgatggaact ccagaagatg 112800 caacaacagc agtggttgga aaagctattg taggtaaagc tgttattcct aattaaaaat 112860 ttgatttttt atgttcaaca tgatataatt aattataaag ttaaataaga aatattaaaa 112920 agtatttatg gaggatttta ttatgacaaa aaatgaaaag caagaaaaga aaatttttaa 112980 aagtttttct cgaaacttaa atgcgttcct taaaaaggaa ggaattttcc caatcaagaa 113040 aggtgtccat accacaggag ttcaaattag tccagatggt gggattactt gggaagatta 113100 tacaagcatt gtgcaagctt tagataaata ttataaagta tttagtcgtg aagatttaga 113160 agatttagtc aaagaatctt ttaaacatag ttcaccagtt tttggagcag aatataaagg 113220 aaacgatatt atggtttgta aacgagttag gtattatcaa gagtatgaaa ttaatgatcg 113280 tttacaagta gctttaaaac gatggaaaga gtctggtcca aaaaaataaa atttgacttt 113340 tttagttaaa taggttataa tattaatata atataaaaga taaacaaata ttggaggaaa 113400 ttaaattatg gcaactttta aaaatcaagt aaaaatttca ggtacattag aggatttcga 113460 tttcgcagtt aaagagtata aagataaaag ctctggtgaa ccgtacaatg caatggttgg 113520 atcattaact ttaaatactg gagaccaatc tgtaaatctt caaggttttt atcgtgaatt 113580 gaacaaagat ggttctacaa atcaacagtt cgcaactatc agcaattggt tacaaaatcc 113640 agaagcattc attggaaaaa attatatgac aacaacttct attagttcaa gtgcttttaa 113700 aaatcaagct ggtgatttaa ttgaagcaac tcaaattcga gcaagcttct taaacgatcg 113760 aaacacaggt gttcctcgtg cagaatttac aacagacttg ctattaacag ctcaacctgt 113820 agaagaaatt tataaagatg agcctactgg acgatatgta ttgaatgcaa acatctttga 113880 ttatcgtgac gtttgtttcc cagctaaatt tattattgaa actgaagctg cttacgatta 113940 tttcgttcag cttgacgcaa gtcaaacaaa cccagttttg attgaagtat ggggtaaagt 114000 gattaataat ctaattcatg aagaacgtga aacagaatca gcgtttggtg atactcgtat 114060 tgaaatcttt gaaagtacaa aacgagaaaa tgtaattact ggtgcagcta ttgaaccaaa 114120 agaaattaca gatgaattat ttaaaacaat tcaagatggg cgtaaagctt acgaagtttc 114180 aattgcggct ttagacaatc aacaacgtaa aagtggattt gcagctgaca catctaaaaa 114240 agatacagca ccaaaagttg acagagcaaa gacttttgaa ttctaattaa tgctctactt 114300 aggaagagaa gcttcggttt ctctttctag ttggaaacat taataaaata aaataaaaaa 114360 gagaaaagga tagtgataca atggcaaaca caattgacat ttttaatgtt gagcctcata 114420 aagtttctaa agatttaaaa ggatatgcag taatgtttta cggagaacct aaatcaggta 114480 aaactactac agcaacacga tttcctaagt ctttgctatt ggcttttgaa aaaggttatt 114540 ctgcaattcc tggcgtaaga gcattgccaa ttaacagttg gtctgaattt aaaaaagttc 114600 tacggcagtt aaaaacagat caagccaaag aaatgtatga taatattatt attgatactg 114660 tagatattgc atacggacta tgtgaaaaat ttattgttaa ccagcatggt gcgtcagata 114720 ttgcagatca aaagaaatta ccttatggta aaggatatca attagtagaa aaagaatttg 114780 atgaacaatt acgttctatc atggctgaag attatggatt agttatgatt tctcattctc 114840 aagataaggt ctttaaagat gaatctggcg aagaatataa ccaaattatt ccaacattag 114900 ataaacgtgg taataaaatt attactcgta tggctgatat tattggttat tcacgagcag 114960 taaatacaga agaaggtttg gaaactcgtc tatatatgcg aggaacgcct cgttttgtcg 115020 ctggttctcg ttttgatagt actcctgatt atatcgtatt taattatgat aatttggtag 115080 gagcaattag tgatgcagtt tctcatctag aacaacaatt tggggttgat tctgtaacag 115140 ataattcttc tacagttcat aaatacaata aaattgatac tcctatcgaa gaattaattg 115200 aaacgttcaa taatcttgct ggagaattaa tggaagctaa tgctgatttc tatggtcctc 115260 gtattcgtac aattgtaaat gaagctttag gtcatgataa taaagtagcc gatgctacac 115320 ctgaacaagc tgaattagta gatctagcaa ttacaaactt gaaagatttg gctgcttcac 115380 aacaataaga ttaatttgat gagcgaggag gaataacttc ctcgcttttc ttttttaagg 115440 aggatacata taatggctaa acaaagaaaa gttatttgtc aatattgtag acaacatttt 115500 agcagggaag aagaactgtt tgaacatact tccaaaggat actatcataa aacttgttac 115560 gatacaatgc aggaagaaag agccaagaga gcaggtataa ctgatttatt agaacagtta 115620 acgcctggta aggttaatta tcctctcgtt caaaaacaga ttaaagagtt tactgaaaaa 115680 tttaattaca ctgaatctgg tctcttggga acactttatt atttaattga tattaaaaag 115740 aaacgattaa cccctgaaag tggtatagct ttacttcctt atttttataa tgaggcaaga 115800 gattattata aggggctaaa acatttagaa caggttacta aaatagaata taagacaaaa 115860 gaggttgttg ttcctccaca aaaaactaaa cgactagaaa aattaattga tttagattct 115920 ttattatcag aggaggaaaa taaaaatgtt taagcaatgt tttcggattt atgtaaaaga 115980 tatcagtgca gacacaaatc aaacagaatt aatttttatt aaaggaaaag acaaagatga 116040 tttagaaaaa ttgtttgata caattatttc tgatttacaa gaagataaga ctaaaagaat 116100 tactattgat atgagtggag tgaagtaaat aaatgaagta ttatcgtaaa gacattgcta 116160 atatgttatt aggagcttta tatgtacaac caacattatt cgatcatcct aagatggctg 116220 cggtgaacga agctgatttt gtggatagct tacaaaaagt ttcttatgca attatgtata 116280 atttatattc aatgggacat ccaaaattta gcttaggtat aatagaaagc tatattcaag 116340 gtcgtccgca aattaatact ttatttgatg aagagataga aattgctgaa ggaaaatatg 116400 ctcgacaagg tgaatattac tttgataaac tatcacaagt aggagatcca acagcttttg 116460 agccagccct agacacaatt aaaaaattaa ctctacttcg ctctcttgag aaaaatggcg 116520 tatctgtaaa acagttttat gattgggata cagagaatca agaattgatt gcttatcaac 116580 aaaattggtt tgaacatact tctttaaagg atatggcaaa tgagatttca gataatattc 116640 aacatattat ggatacttct gcaagtaatg tagcttctga aagtatccaa gctggtgaag 116700 gattattaaa tttaattgat tcttttaaag aagccccaga ttttggttca ccttctccaa 116760 ttacaatgat ggatacgata actcgtggag atcgattagg taaattctat ttgtattcag 116820 ctccaacagg gaatgggaaa tctcgtatta tgatgtctcg agcttgttac tctgccttcc 116880 ctgtaattta tgaccaacat aaacaagctt gggttgacaa tggagaagca gaaggttctt 116940 tatttatttc tacagagtta gattcagaag aatgtcaaac aatggcatta gcatttctta 117000 caggaattcc tgaagatata attttagatg gtaaatttga cgaagaccaa gaaaaagtat 117060 tgagagaagg agctagaatt atgtctgagg ctccgttata ttttgaaatt atttcagatt 117120 tctctgttac agaaattgaa gctactatta gaaaatatta tcgagaacat gatgttaaat 117180 atttccgttt tgattatatt catacatcta tgaaattctt agcagaaatt gcttctattt 117240 caaatggtat gaaattacgt gaagaccagg tattatttat gttgtcaact aaattaaaag 117300 atttagcaaa tcaactagga atttttattg agtcaggaac tcaggtgaat ggagattatc 117360 ttgagggaga acttaatcaa aatttgcttc gtggatctaa agctattgcg gatcgtattg 117420 acgtgggaat gattgcagtt aaaattagac ctattgatga acaagctgta gaaacatttg 117480 taagtcaagg atattttcgt ccaaactata ttattagctt ttacaaagta cgacgaggta 117540 aatatgctgg tgttaaattg tggtgcgacg tagatttggg aacttgtaga tgtaagggtt 117600 tgtttattac agatagtaat aatatcccta ttggaattga tgctacttct attaaggttc 117660 aaaaagcagc agaaaaaaat gttaagcgtc gtaatcaaac tgtagatgat cgaaaaagtg 117720 ctttttaaag tcaagctaat tgcttgactt tttattttta ttatgatata atataaatgt 117780 ataaaaaata attaaaaaag taggttgtag gtatatggca gaatatggac gtgataagaa 117840 tgctataaaa gctggaatgt ctattcaaca agtatatgat tttttggtag gacaaggtat 117900 tcaaggtaat tgggttgatg aacaccaatt agtctttgaa actgtctgtc ataatcataa 117960 aggtgagggt agtcataaac tttattatta tgataataca aaattattta attgttacac 118020 tggttgtggt agatttgata tttttgaatt attatctaaa atgtatatga atgaatatca 118080 tgaagaactt ttactagaag aggctattga tatttatatt caatcacaag agtttatgac 118140 tttaggcgat aatgatatgt tgcataatga tgatttagat attgaatatc aagaaccaag 118200 ttttatgttt tatgataaag aaccttattt acgttatcct agaattattg taaatgattg 118260 ggaacgagaa ggtatatcag cagaaactca acataaatat aatatcagat ataattatgc 118320 aacaactggt ttaatgtttc ctcatttaga cgaagaagga aatctattag gtatacgaca 118380 aagaatagct aatagcgaaa tgatagaacg gtatggaaaa tatagacccg ttgaacgtga 118440 agggatactg tattcttcac cactatcatt ttatttattc ggtttatttt ttaatgctaa 118500 caatattaga agagaaagaa aagctattgt ttttgagggt gaacaacatc gccctagttc 118560 atctttccac taatcagtgg ggttagcata agctagctaa cggggaagcc taagtcagaa 118620 atgatatggt aatcccgtgg gaagattaat gtagatttca ttcctgatta ttcatttctt 118680 taaagaaaga gaaatgagga atgaaatatg ggaattatat atggatatgc aaatccaaaa 118740 ggaaagattt tatatatcgg tcaaactgtt aatttggagc aaagacataa caaacatttt 118800 aaacacgacc cttataacga aaaggttcta gaatacaact atcctcttag cagaggcatt 118860 agaaaacatg gagaaaaata ttattcttta gttatactag aggaaaatat tgataattgt 118920 ctattaaacg acaaagaaat gttttatatt tctaaatatg atacctttag aaatggttac 118980 aatcaaactc ctggaggaag tattcctccg acaataactt acaaagatga aattatttta 119040 gaggttatta aacttctaaa aactgactta tcatataagg atatatcaga aaaaactgga 119100 ttatcgtttg ctcatataga taatataaat aatggtaaac gaagaaagtt ttcagatatt 119160 gaatatccaa ttaggaaaaa tcaaattggg acaagaggaa gaaaacttac tcaagaacaa 119220 gttgataaaa ttgctgatga aataaaagat ggaataaaat cttttgacga catagcattg 119280 gaatataata taagtcaaac aactgtaagt aaaataaaca gcggagttag aaaaagtagc 119340 tattcaaact ttcctataag acccaagaaa aaacattaat aacctgtatc gactatcccc 119400 ttgaggggag tagggctact attgatacgt agttcgaaat ggtgattcaa acttaggttt 119460 gaacaagaga tagtcagtac tactagaaat agtagaataa tacggagaaa tcggtcatga 119520 aaatggatga tttattatcc ccaaagaaaa atattggtgt agcagcattt ggtatgcact 119580 tttcacgaca ccaatatgaa gcattaaaaa aattaggtgt agaagaaatt attttcgctt 119640 ttgatagaca atttcaatct atgaagtatg atagagaata tgataactta ataggaattt 119700 ttaaaacaat acaagaacgg tttggaacta acgaagatgg tattaaaata agttttattt 119760 tagatactaa tatgatttca ggatataaag attctccagt agattgtggt tttgaagttt 119820 ttgcagaatt atatggagaa cgaaaaacat acaaagagat agatgaaaat tttgtagaaa 119880 aattagagct atgggattcc ataggaacag acgaagagga gtatgatata tattgagaac 119940 gcaactaatt ggaaaaaatg atgacaatat tattaacatt ttcataaata gaggaataaa 120000 cccagataaa atttttagat taaatgattc agcgttgcac gatcctcttt taataaaaaa 120060 tttgaaagag gttgctgaaa ttattcacga caaaatcgta aattcaaaag ataaaatatt 120120 attaattcaa gaccctgatg cagatggtta tacatctaca gctattatct ataattattt 120180 aaaagattgt tataaagaag aaataacaaa tagaataata attattttcc aagagaacaa 120240 agaacatgga attcctgtaa aaacagtaga agatattatg ggggaacatg aagtagattt 120300 aatcatagct cctgactgtt cttcaaatga atatgatgtt cataagtata ttttagaaaa 120360 gttaaatata cctattgtaa ttttggatca ccacgatgca cctaaatttt cagaatatgc 120420 tacaatggta aatatttctt tagatgatta ccctaataag aaattgtcag gtgcaggagt 120480 ttgtttaaaa tttgctgcgt tgtatgataa aatgtatggc ttcgaccact cagaaaaata 120540 ctatgatctt gctgctattg ggattgtagg agatatgatt gaaattaata ctttagaaac 120600 aatttatatt gttcagcaag gaatgagaaa tattcacaat acttttttaa aagcattata 120660 caaggctaag tcatttaatc ttggaggaca agtaacacca atcggtttat ctttttctat 120720 tgtcccaatg attaatgcag taacacgagt aggaacaatg gatgagaaaa aagaagttac 120780 aaaagccatg gttagtgaca aaccttatga tgtaccctca actaaacgtg gagctaaaga 120840 gggagataca gaagtattcc atgaagcagt tgttcggaaa atgaataata ttaaagctcg 120900 acaggataga ttgagagata atgcctttaa ttcattagaa actcaaattg aagaaaataa 120960 attaactgac aaccagattc taattttaga tacaaaaaat gaatttccaa aaacctttac 121020 aggattaatt gctaatcaat ttgtttcaaa atataagaag ccaactttag taggaacgta 121080 ttttgaaaaa gatggaaaaa ctttctttgg tgggtcagct cgtggagatg ataaatctga 121140 tttaccagaa ttaaaacgat ttgagcaaga aagtggatta tttgaatttg cagaaggtca 121200 tgagggagct catggatttg cttttgctgc tgacaaaaag gatgaaattg tagaatattt 121260 taataaacaa ttatcagaaa ttgtctttga accaatatat tctgtagatt ttgatatttc 121320 ttggcagcaa atgtctaaag aaaatctaac agaaattact cgttatgcta cgttttgggg 121380 aaaaggtttg gaagaaccta cctttgtttt aagagatgtt cctgttaaaa aagatgatat 121440 agttgtgggt ggagaatttg ataatcacaa gataacattt tctgctgggg gactagagtt 121500 tattaagttt aagggtgtta gcactgaaac agctcaaaaa cttatgaaaa accagcaatc 121560 aaaaattgat gtagttggaa ctattaaaat gagtactttt agaggtcgta cgttcctaca 121620 aatgtttgtt gcagatttag aattgaaatc agcgcaaaaa tacattttct aatattaaag 121680 gattttatcg tctacggacg ctaatctagg agaaaaatat ggttcatgct attttttgaa 121740 ttgaaccata tttaccttct acctattttt cttttcctaa aaccaccccc ccttacatat 121800 aaatttaagg agtgatagat atgaaacgca aaataaccaa aaaagaattt gtttcagaat 121860 atttaactcg ctacccaggt caaatgaaaa atgctttaat tgcttgggaa caaattaaaa 121920 ctaaacgaaa taaaaagtca agtaaataac ttgacttttt atttttataa tggtataata 121980 taaatataat gaataaaaaa ggaatgatta aatgtcatta acaagtaaac aaaaacaagg 122040 tttagaaatt gctgtagaac gatataaaaa tggagagcgt tatactacaa tcgctggttt 122100 tgctggaaca ggtaaaactt ttttagcaaa aaaaattatt aatgcactag agctatcacc 122160 attacaatat atggttggag cttttacagg taaggcagct tatcgtcttc aacaaacagg 122220 attcccagca gcacaaactt tacacaaatt gcgatacaag tctatcaaaa ttaataataa 122280 ttttattcat caaaagcttc ctattgaaaa ctatcaagga attaaattaa ttctaataga 122340 tgagatttca atggttccag aagacctgtt gaaagatata gcagatttag gtattcacat 122400 tataatgctt ggagacccag ggcagctacc tcctgtagga gcagataatg gtatgttaaa 122460 gaatcctcac attttcttag atgaaatcat gcgccaagag gaagggaata gtattattca 122520 attatctaaa atgattcgag aaggggaaat tataaaacct ttttctgatg attttgttaa 122580 gatggttcca aaagaagaga tttctgtagg tatgctttta tgggcagacc aagttttgtg 122640 tggaagaaac gcgaccagaa aactttacaa tgatgaaatc cgtttagcta agggttttgt 122700 ttcacctact cctcaagaag gagataaaat cattgtaact aataataatt gggatatttt 122760 aaacgaagaa ggattccctc ttattaatgg tatgttaggt gaagttaatt tttctagtgg 122820 tgtatttaaa agtgaaaaat taatgggaaa tgcaatggga aaagatattg agatcgctcg 122880 agttcgtttt gttccagaat ttggtggtgg agagttttct atcaagtatg atatgctccc 122940 ttttattaaa gaaaaccaaa aatcttatat tgtaaatcag tttgcaaaag gtgatgctag 123000 aattaattat attgattatg gatattgtat tactacacat aaatctcaag gtagtgaata 123060 ttctaaggtt ttaggaatcg aagaacgatt aaatggaaaa aatcatagaa aatggttata 123120 cacaatggtt actagggctt cagaaaaatt agtattagct tatgataaaa atagtggaat 123180 ttgggagaaa taaatatggc aaaattatat tttaaatatg gagttatgaa ctcagggaaa 123240 agtttatctt taattacaac tgcttataat tatgctcagc aaggcaaaaa tgtattaatt 123300 tatacttctg atttagacac tagaagcggt tttaaaaaga ttgtttctag aactggtttt 123360 aatattgagg cagagtatct aaatgatagc agagaagaaa ttttagaaaa ggctaaaaaa 123420 gagaaagtcc atgctatctt agtagatgaa tcccagtttt tgtcaagaga gcaggtaatt 123480 actttagccg gagtagtaga tgagggcggt attcctgtcc tctgttgggg gttaaaaaat 123540 gattttcaaa ataatttatt tgaaggttct caagcattgt tagaatttgc agataaaata 123600 gaagaactaa aaactgtatg cacattatgc gataaaaaag caactatgaa tttacgtaca 123660 attaatggag aagctattta tagaggtgaa caaattttaa ttggagataa cgaatatatt 123720 cctgtatgta gaaaatgtta ttttaattat gatgaattgg tcaagtaatt attttacttg 123780 actttttata ttttaaatga tataattaat atataaggaa ttatgaaagg agttatattt 123840 attgggatac tattcaattc ataatcatag tagttttagt aatgcattgt taggattcgc 123900 tgattcaatt aatgatatta aacaatcagt tgattatgct ttagatttag gtttaagtgg 123960 ttatgtttta acagatcacg aaggattatc tggtcacgct caattaatgc attatagaga 124020 taaattagaa aaagaaggtc gtatagacca tgaatctttt acaattggtt ttggagatga 124080 aatttattta gtcgaagact tagaaatggg acaaaaatat tatcatcatt tattaattgc 124140 aaaagataaa aaaggacaca aaattttaag aatgttgtct tctaaggctt gggaaaatag 124200 ttattgggat agaggtttac aacgaactcc gattactcgt gaacaagtaa aagaaattat 124260 ggaacaagaa aatggaaaag gacatatcgt atcttctaca gcttgtcttg gaagttttga 124320 agctcaagct tttttgaaaa ttaaagcggc agaagaagtc ccatttggag aaaaaattaa 124380 tcaagaaaaa atcttagaag aaaaacaaaa aattgttgat tttgttttgt ggaatcaaga 124440 agtttttggc gaagaagatt tttatttaga aattgctcca aacacttcag aagaacaaag 124500 atatgtaaat agaagagttt ggaaacttgg gaaagcaatg aatgttccag ttgtatttag 124560 tacagatagt cattatctta ctgaagcaga cagagagatt cataaagctt atttgaatag 124620 taagcaagca gaacgagaag ttgacgattt ctattatact gcttatatga tgccacctga 124680 gcaagttaag gaatattttt tattagattt ttcagaagaa gaatttcagg aaatgttaga 124740 taacttagaa aaaattagaa gtggaatcaa acattattct ttgtttaagg aacaagaaat 124800 tccatatgtt cctgttgatg taccagaatt aagtaaaaca tataatgata ttgatttaag 124860 caaatataaa tatttaacaa tgttgttaaa ttctgatgaa gaccaagatt tatattggag 124920 cagaacttgt ctaaaccaat tagaaattag gaatgtatta aatgaaagat acttagaaca 124980 attggaaatt gaagctacta cattagtaaa aatttctgaa cgtttaaaac aacctatgac 125040 atcttattat aatacagctc aaaaaattat tgaattaatt tgggatgaag gagattcatt 125100 ggttggagtc tctcgtggtt cagctatggg atttatgtca aactggttgt tggacgttac 125160 acaagtagat cctatcccat atgtaggaga tatgagttgg agacatctag ctgaaagtcg 125220 tccagaattg cctaaactta ttgggcattg aatgtgaacc ttgctaaagg gtgtacgatt 125280 aaataaatcg tgctaacggt aaaagttgaa taagacttcc acacaagcgt tacaacgaga 125340 tggtggagat ataacaaaga cgaagcagct ttctaagaga gtctaaggtc ctagagaata 125400 ggatagctgg ataataccgt gcgaagcttt atttcaaagt tctccaaata attaattgga 125460 gatgataaat tgctatatga tattgacgga tataatggag attataaggt agatagagag 125520 ggaaatgttt ggagctttaa aatgacaaaa ccaaaaattt tagcaacttg gtctggaaca 125580 acaagtcctt acaaacaagt ccagttgtct cgaaatggga aacttaaaaa atatttggta 125640 catcgtttaa tagctgaaac tttaattcca aaccctaata atcttcctca agttaaccat 125700 atagatggaa attgtttaaa caattgtgtt gaaaatttag agtgggtctc tccacaagaa 125760 aatataaata agtcttatga gactagtggg attggatcca cacgaaattt tcgaaaagtt 125820 gagctttttc aaaatagtgt gtctttagga atatatggtt cggtaaaaga agcatgtaga 125880 aaagctcaag aattaggagc aagttttaca agcttagaaa aatatagaaa aactggagac 125940 tttgaaataa agaacgtgta acgactagag ttttatactc gtagggatgg agataagcac 126000 cattccgaag tgcattcact ttacgtatat acgtaaggaa gatatagtct gtgccctagt 126060 gaaaactagg gagaacatgg atgttgatat agattcacaa ggttctaaac gagaacaaat 126120 tttattggcg ttaaaaaact tttttggtca tgatagagtt ttaaatatcg caacctttgg 126180 aactgaaaaa gcaaaatcag cattagctac tgctgcacga ggacttggta taagtgatga 126240 tgtttcaagt tatttaagtg gtttgattcc aaatgaacgt ggctttgatt ggaatttaaa 126300 agatatggtt tatgggaatc cagaaaaagg tcgtaaacca gtttctgaat ttattaatga 126360 aattaataaa tacgaaaatt atttagaaac agctttagct attgaaggaa tggtaaaaag 126420 tagaggttct catgcttcag gagtttatat ttttaatcat ccttatacag atattaatgc 126480 tatgatgaaa actcctaacg acttagaagt aactcaatgg gattatcatg atagcgatga 126540 tatgggagca ttaaaaatgg atatgttgtc tatcgaagct ttagataaaa ttcgtcaaac 126600 aatggattta ttgattaaag acaatagaat ggaatggaaa ggttctttaa aagaaacata 126660 ttttaataca ttgcatcccg atgtattaga ttatagcaaa gaattatggg aaccaacatg 126720 ggataacaaa gttttggata tttttcagct tgatacacct gtaggaaaag aagctgtaag 126780 gaagggtaaa ccagaagatg taaaagatgc agcagcattg aattctttga tgaggcttat 126840 ggcaactgaa aatggtgaaa tgccactaga aaaatatgca agatttaaag agaatatcca 126900 actttggtat gaagaattag aaaaatataa tattccagca gatgaaattc ctatcctaga 126960 gaaacatttc ttatcttctt atggggttcc aaatacacag gaagaattaa tgttgatttt 127020 aatggataaa aacatttgta attttacaga aacagaagct aatgcagcac gtaaaatcat 127080 tggtaagaag ctaatggata aaattccaga acttagagct aaaatttttg aacaagcagt 127140 atgttcaaat gaaacaattc attatatttg ggattcagca gttagtgttc aaatggggtg 127200 aataagaacg cccttaaatg gtttttccac tgtaatcaag tggggtacag ttttacttat 127260 tttaacaaaa tgagtatact gtgctaacgg ggaaatccta ccaggtaatg ctgaggacaa 127320 tcccgtggga agttttagta tggtatcctc aataggagga tataaaaatg ggttatatat 127380 atgcgtatat aaacatgttt aacggaaaaa gatatattgg tcaaacaaat aatatagagc 127440 gaagaaagag agaacatttc tcaatggctt ttaatgaaaa aactaaatgt tataattatc 127500 cattgtatag agcttttaga aaatatggat atgaaaactt tgttcatgtt attttggagg 127560 aaactgatta tcttgatgaa agagaaatat actatattaa taaatataat actacttctc 127620 ctaagaatgg atacaatatt catcgtggag gaaacgaaag tattatagaa ggttctctta 127680 gtatttctca ggtagaagaa attaaagagc gattagaaaa tggagttagt tatacagaaa 127740 tttccaaaga atactctatt tcaaaaactt taatttctaa tatcaatata ggattaagat 127800 attttaataa agattggagt tatcctttaa ttaaaaatta tagaacttct tctgactacg 127860 aagaattaat tgttttatta aaagaaacaa acttatcttt tgcgaaaata gctgagagac 127920 ttggtataag tgtttctacc gttaaaaaaa ttaattatgg aaaactaaga aaaggattga 127980 gtgatactta tcctattcgc ccatatacta aaaaacctgt atcgactatt ccctaagtga 128040 agagcaggga agtagggcta ctattgatac gtagcatgat tttaggaaac gaagtcattt 128100 aaatgccgaa atagccattg ctcacataac gtgagttaag atatagtcag tgcctatagt 128160 aatataggat atacacgatt ctttctcaat tttgcatacg attggatata caattattgc 128220 actacaagaa ttaaacttat ttaatcatta tccatctatt tattggaact gtggttgttt 128280 aattgtcaac tcaggagatg aaacaggaac ttcaacagat tattctaaaa tggcagtagc 128340 actaggaaat actattaaac atgggattaa agtagaaatg gtagatataa ataaatcaga 128400 atcaaacttt tctcctaatg tagaaggtaa ttttatttct tacggtttaa gaccattaac 128460 tgggattggt tatgaattta ttcagaaaat tttcgaggga agaccattta attctttagc 128520 agattttgtc agaaaaacaa acccaaataa aatgcaaatg ctaacattaa ttaaggcagg 128580 agcttttaga gaaatagata gtcctgatag aaagattact ttagcaaaat atgctaaaat 128640 gatgacaggg cttagaaaaa atctcacaat gacacaagtc ccattggtta tgaaaattaa 128700 tagattccct gaatcattac aagaagaaaa acgaatttat gagtttaacc gttatataaa 128760 tgaagttcta accaagagcg gacaaattgt tagacttgat gaccgggcgc aaaacttttt 128820 atcttcgatt ggtttagatg aatttattga aacgtttagt gatgaggatc gttattggac 128880 tgtgatgagt cgagatagat ggagtaagtt ttatgacaaa aaaatggata ctgttagaaa 128940 ttggttgaga gagaataaag agtctttatt agaagaaata ttttttgatg aaatgttaga 129000 attatttgat tcatatggtg gtcacgattc atatgcaaga tgggaaatgg aatctatgaa 129060 cttctattat acagaacatg aattaaattg tgtggatgta gatagatatg gaattgataa 129120 ttttgagctt cttccaagag aggcacaaaa aatcaatcct tacgcaagtt tttctaagct 129180 aaaattaaat agaattgttg gaactattat tggtaaaaat aaggttaaag caagtatcga 129240 tgttttaact ccagaaggaa ctgttgtttt agttaaaatg tacaaaggag atttttctta 129300 ctgggataaa caaatctcag aaagaacatc aacagggaag aaaactgttg tagaaaaatc 129360 tttctttgaa agaggaaaca aattatttat tactggtttt agacgtgacg atcaatttat 129420 tcctagagca tataatgata cagcgactcc aaagattgga ttaattactg aaactcgtga 129480 tgatggaagt attgttttga aaacagaacg agcagcctaa tccgacgagg ttaattatct 129540 tacgttaaag gtctcagtac ttaaaactgg gatcttggcg aaagattgac aaataatata 129600 taatatgtta taattaatta taaagttaaa acaaggagag ataaaatgtt agtaatctta 129660 tatggacaaa caggagctgg taaagattat attgcaagga aattacttgg ctctcttggt 129720 gtagatgctt atctagccaa acgaccaaca acaagaccca agcgttccga caaagaagaa 129780 tcttattatc aatttttaac tgatgaagtt tttcaaagag gagtagaaaa aaaagaaatt 129840 ctatttccta aggtatttaa taattggaat tatggtgttt actttggtat tactgctcag 129900 gctcgagata aagaatctat ctatttaatg acttgtgaaa aagagactgc atttgaagta 129960 gctgattggg taggtaaatc agaggataca aatgttatgt taatagaaat tgtagcggat 130020 gaaaagattc gaatggaacg agcaaagggc agacaaaaga atcctgacgt aaatgaaatt 130080 aaacgacgaa ttgaagcaga taaaaaagat tatgctgaaa ttgatagaaa gccagatact 130140 tactattttt cagatacacc ctttgtatgg atggcattaa gtgctttaca agataaaatt 130200 atggaaaaat taaaggagaa taattaatat gaaaaagaaa aataatggac aaacaatgaa 130260 aaaatcggat ttaattacac aagctttaaa ggacgcaccg aaattatcag aagaagatat 130320 ttatcaagcc gttctaactt ttgttagaaa atcttttgga aaatattttt ggattaattc 130380 acgagatatt aactataatg tagtttttga tcgaactgag tctacagata aagaagtggc 130440 tcaacatatc ttaggttatt taaaagattc tagtttttgc caaccaaaag aaaatattaa 130500 agataaaaat gaaaatgata ttgtagaagc tgatatgatc gatatgatgg atgtacgaga 130560 tattgatgat acttctcatg gagagcttgg actctatatt ggacttactt attttaagtt 130620 agttagcaac gattggattg ttgagtcagt taaagatttt acgcaaaaag ataaaaagga 130680 gactaaataa ttatgacaaa agtacgtgga tttgaagtag caaaaggttt tgaagataaa 130740 aatattaacc tgcctgttcg agcaactgct cgtgcagcag gatatgattt tgaagcatca 130800 gatgatattg caattccttc agtttgggat attaaagaaa ttcataagaa atcaatttca 130860 agtatccttt ctaaagatct atttgggttt aatattgatt tacaagatcc agattcaatt 130920 agtcaagtta tggaacattt aacagaagtt gaatctttag aactattttc taaagttaca 130980 gatttcctta ccaagattac tcagctttta aaagataagg gtatggattt agaagaatta 131040 gcttcagacc ctgcctctta tacagaatta tttaatactc taaatttaga aaatcaaaac 131100 aatgaaattc taaaagagtt attaagtgat gaagctattt cagaaattaa aactttaagt 131160 aagcaattta aaccagtttt agttccaaca ggagtaaaag cttatatgga gccagatgaa 131220 tatttagctt tatttaatcg ttcttcaaat ccattaaaac gtttcttagt tttaacaaat 131280 ggtgtgggag ttattgatgg tgactactat agcaatcctg ataatgatgg tcatattatg 131340 ttccaatttc taaattttgg tcctgaaact atctatatta aaaaaggaga acgtattgga 131400 caaggagtat tttcaaaatt cttgttggta gatgacgata atgctcaagg agaacgtctt 131460 ggcgggcacg gaagtacagg agtaaaataa ttaagaaaga tattcttctc ttatatagga 131520 caaaaaaaga caacatgtct cacctctttg ctatttatta gtagggaggt gttttaatat 131580 gatttttata ggtttagacc aagcaacggt tgtaacaggt tattctgttt ataagaatca 131640 agaattaatt gcctatggaa aattttctgc tgaaggagat gatttctcaa gatattcaaa 131700 acagaaagca aatgtattag cgttaattga atatactaaa gaaaaatatc ctaaagaaga 131760 aataaaaata atgtttgaag atattcaaat gcagcaaaat aaaactactt ttaaacaatt 131820 agctcaacta caaggcgctc ttgctgttgg ggttttagag gctcatccag aaattcattt 131880 tgattttatc tacgcttctc aatggaaagg atttgctaag attaaaggta aaaatagaat 131940 agaacaaaaa agaaatgctc aggctaaagt attagaacta tttgatatta ctgcaacaca 132000 agacgaggca gatgcaatta tgattggcta ttatgcttca caccaagaaa ttaattggaa 132060 ctaaaaaaga gaccacatag gtctcttttt ttatatagct ggatttgttt gtattgcaga 132120 aatattcatt gttggacttc cttctgtgtt taattgtata gaatatcctg ttatcataaa 132180 cattcctgat atatttgaaa tttcatcttc tgcataaatt aatgtatttg gttctaaatg 132240 ataaactggt aatacatcta ttgttactac atctgaagta ttagtatgtt gatagattaa 132300 attcctaaca gaagaaaata aatcaaccgc tccatcaata gtaaataaat tatccatttt 132360 agaatctcta atattaaata actcttgaaa atcatcaatt ctaccacttg accaatcagg 132420 aaaatctgca cttcctttat aaacagtttt acttaatact gctaccaaaa agtcgttggt 132480 tttatcccaa ttaaatttag tccatgcagg ttttccatta ttttgtttag catagtacca 132540 ttgagtacct atatttttaa ctacagcaaa ataattatga tttccactat caggacagcg 132600 gctgtttttg tttaatagat aagcaataaa accatattcc gttaaatctt tttcactacc 132660 aaaaggagta tttatggttg caaaatctgt ttgagtaaac tgtctttttg taactggatt 132720 ggtgtatact ccagcagaaa ttgtaacata tccaggctta tcttgatctt gcatattctt 132780 atttacccca atagtaccag tatgtttacc accagctaaa tattcatgaa ctgttccatt 132840 gccttctgca aacatatatc ttaattgttc aaaccgccct gggtctccaa ctatagcact 132900 ataaggcata acatttttat tttttgttat tgctgttggc ataaagaatt gatcttgtat 132960 atctctaatt actgcataag cttctccttg tgcttttaaa ttatctaata tcatatctcc 133020 gcctaaatct tgtaattctg tttctgtaat aacaattaat tctttagggt ttgttctaaa 133080 aatggtagtt gcctgtttat tagaaacacc aatagaacgt ttaccaatta actcaataga 133140 atatttaccc aatttagatt ctgttggtat aatatcaaga aagtaagtcc aataagccga 133200 atcacctttg tcagtaaaaa taggattctg tttatctaaa gactcaacat cattttctgt 133260 gtttgcgctt gcagccatag gataacctgc taaccatgga gttccgacag aaataaagga 133320 tggattaaaa attccagtat ttggcaacaa agttttttta tcttcagatg caatccattt 133380 aaaaatcatt gattcgcatt ccttacccca tctaggcaga actcttgcag tagaagcgcc 133440 atagatattt ctaataaaat aatttttaag tcctaaacca atttgccatg ggattttatc 133500 taaaggaact tcaatatatt gtgttggttt ccctgctgtg gcttctttaa attcaaaagg 133560 aactgtatta gtttgagggt tatatggctc ccttcttctt ccatcttttg ctaaaaaatc 133620 catttctgga gaatccatat taaaatcttc tgcaatccca acaaaccatt ctttaatctc 133680 tctaattgtt ggtttatggt ctattgctat ttctaaaatt tctcctgttt ctctagttgc 133740 tataaaatca tttttgatat ttgtataact aggattgttg ttatatctaa taatagtctt 133800 tttatcggca aaattatagg taaaagggag acctaaataa ttagggataa aatcatccat 133860 gtctaattca taagcaaaat atcctaaatc tgtatctttt tcagggtcaa atacttcatt 133920 aacataattc ttgataggtt gaaacattaa atccccatca cgagtataga aaaattcatg 133980 agtattgctc atagcttcta caatatcttc aaaaatagag gttaatggtt catttacatt 134040 cttttgataa gattcttggg tacttactgt tcctgctgaa gggtcaggtg gaccaaatct 134100 gcgtaatttg tagactctat ctccctttga aaaattaatt tctgtttctg gaacagttgg 134160 actccacgcc ctagtaataa ttctttctcc ttctacatct ccaggagcgt taatatgaat 134220 aaatttatct tttagtccag ccacattttt tacttgcgta tattcattta tataatctgg 134280 gacagaatca ataataacct ttgctggatt ctcattccca aaatttactg ctgcgcttaa 134340 gaaaatatct ctccaagaga aggaataatt cttaccattc cttttttcta caaaacttac 134400 tggggctgga aggcgacccc ctaatgtacc atttagcata gtcattttat cttgcgcgtt 134460 aattgatatg gtcgctgtat caacagaatg agaataagat ggttcggtta aaataaaaat 134520 ccccatatta aaccatataa tttcatcttc ttgataacga gaagtttggt tttccaatcc 134580 tatatataat tttatttttt tatctggagt tagatattta tagactaatt catcgtctct 134640 atcttttaca ctaaaggtta aagataaatt tcgtcttact cgacttgttc cgtcagcatt 134700 ataacttccg ctttttacaa taccttgtat ttcttttaaa acttgttcgt cccaattaag 134760 taactgtatc ttagtatgca ctactcgaat attatgctcg aaaagctctt ctagaaaaga 134820 agtaggatac tgcttaacat taatattttc attcattatt ttctctcctt tgctcttggt 134880 atgttttata taaaaaaatt aaactgggta ctatcacaag ttccccagct taattttatt 134940 tattagaacg aagtattttt catagcttct tttacaagct catccttcat aatagaagat 135000 ttacgaagtt tatatttttg taatgctttt tcatcaagtt ttccaatttc agtaacagta 135060 cattcaaaat cagaaattaa accaaacgtt ggttcatttg gagtaacctt aatatttgtc 135120 aacattacta acatatttcc ttgagcttct gatttaagaa ttttaggttt tccatcattt 135180 aaccatgcta gaattttgtt tctccaaatt ctttgcatta ccatgccatc ttgtacttct 135240 tctaaatcgt tcttcatatt aagcaacatt tcatctccat attttagttc tacataacta 135300 ataggaatag ttggatctgg agatattgct acagaataac tatcagaagt aagtaatttg 135360 tggacatctt ggtgataggt taacgtacca gaaaattgat atgttctata atctaattca 135420 gaagaggtta caaaatatgg atattgccct gcaatagttt gaataacatc tctaggtttt 135480 acataggaga tttctccaat tttaccattg taaataaatg aaaactgttc atctttttct 135540 cctagcaacc agaaaccatc aaaagtagtt acagtatcat aaaatgaagt aatagcacct 135600 ttcattccat tggctaatat aggttgcaaa gcataccgat agtaatttcc attttctata 135660 aaataatcat ataagattgc ttcttgtact gaactagatt ttagtctagt tacttcttcc 135720 catttcagca atccttctcc caaaacagaa cgcaagattg caatttcaga aattaattta 135780 gatttatcaa cctctccatt cagcattgga acgtctattt ctcctgcatt aagatttaac 135840 aataattctt cttcatgcaa attccatcct gtatcttcca tacctatttc tgctttaatt 135900 ctatttactt gtaattcact ttttcgatct tctactccaa gaatagaaac tctaataaaa 135960 gcagctgctt ttgttcggtc tgtaaaaata aaaggatgtc caaaatgttt actttcttca 136020 gttttattaa agaatctagg agaatatttt gtttgaatta attgtctatt attatcatag 136080 aattctatac gaattactgc tccacctgaa tcagttttct cactaaggtt aaagccaact 136140 gtaatagcat ctccttgcca cagaactatt tttaatggta caaattgagc gattcctatg 136200 ttagcattaa ttttaggagc tttataaatt ctaaaggcgt ttccatttct tttattttct 136260 actaatccaa cctcaccttt aaactctttt tgtttaaaat cataataatt tttccagcca 136320 ttttgataat atttaggttc tatagtttct tctcttgtat ctacccaaat tttactatgg 136380 tcggaaggtt cagatttaga ataaatccaa ttataaggtt gttcaatatc atcagaaccc 136440 tctacataag gtttttcaaa aactgaggtc caatgagtac tttgaaaggt tgataaagct 136500 gtattcttta ataggttaaa attacctgtt ggagattctc ctcctgcgtc aacaaagtct 136560 gattgtattt ctgtaactcc agaagcccaa tcataacgac caccatccca atctaaatta 136620 ggctccatat gaccaccttc catatatata gatttaattg aaatatctct taaagctata 136680 tcagaaagat aactatctcc agcctgtgga atagctggag aagtcattaa taagggatag 136740 tatagttgta agtcgtcatt accatattct ttaaatgaag caaatggtac tgccatttat 136800 atctacctcc ttattgtgga taatcattta agtcttcatc agggttaaaa gtaattgccg 136860 gagcaatata ggtatcgtct tgtggttgag cccacaaata tccaagagca gaaggtcctg 136920 ggctattttc gctataaatt actggcgcac cccaattttt attagctgct acttttgcta 136980 gttcatcttg aagccataga ctccattcat taatttggtc tactagttgt tgagcaacaa 137040 ttgaaattag ttcatagttg ccatctgccg cttcaattga tgcttttaaa taagactgtg 137100 tagttctaat agcatcagcc attagattaa aatcatcttc cgtcaaaaca taattacgaa 137160 ctctcaacat caactcttta agttttacaa agtcttgctc tgatgctgta ttatttgata 137220 cattcgctaa gatagcataa taatcatgta agtctttttc tagttgtccc ccagaaggaa 137280 cacttgaata ttcaattact tttaattgat ctacaacact agggaattta gaaggtccca 137340 cattagctgg aggatcaatt tctaatcgtc tgggtacttg tatttttaag tctactttat 137400 ccattattct atttctcctt tactctttaa ttcttcaatg tcactggtta acaagtctct 137460 cgaacgtgtt gttgcagcag agatgtttcc aacatccgtt cctaagactc ctcgttcatc 137520 tgctgtaatt gtataaggaa cttccttacc acttaaatca acttttggta ggaaatctga 137580 acctaaccca gttgttgtta agaacatatc agaacttatt ccaacactgt tcatgaatcc 137640 ttcagaataa gttaatgtaa atctatctaa gctattttgg ctatcatcag cggataaatc 137700 tcgtactatt aatcttatct gtccatcaat acaagaaaaa tacatataat attttcttgt 137760 tggttgtatt ttaacccact tatttaactt cttattaaaa tcattcatgt aagccttata 137820 agtttgtgta gttaatagag tttttcttcc aagctcaaga gcataaactc gtttaacaaa 137880 tctaaattca ttaagataca ttggacggac gttttcgccc aatacataat tttcgtaaac 137940 tgtatcaaga acaatagttc catctgtctc tactcttggc actactaaaa tattatgata 138000 tgaatcaaag aaacaaacag gttttaaaaa ttctgttgga tctttttcat gagatttttc 138060 tgtaacgaat tctccattaa ttgtgacagt tgttttccat aattgttgat tttcatcttc 138120 taaatagaag aaggtatgcg aacttggtaa atcttcgctc ggaatagtaa cgatacctcc 138180 catacctcca acaattcctc cttctgttgt aggagtttta gaactgtcta ggtaagttag 138240 atatatatct cctgatggac tttcaataac aggattaata ataatatcat cttcgtacgg 138300 accttcagaa gctttatctt tcccaagagg agcggcatgg gcatacatta gatattcttc 138360 ttgatagggg ttaattgtag aaatctctgg tccttttagt ctgaagaacc atcggtcttc 138420 tttcgcttcc caagttttta ttgggttaat tccagaaact atcatttccc ctgctatagc 138480 agcttgttta ttaaagaagt ctattccttc aagtacagat acttctccat ttgcgtcagc 138540 aaattctcta tcgttaagag aaatttcaaa gttttccttt ggtccccatt gtagaggtgt 138600 ttttatattt agtttgattt tagaacgagc attatcatga tcgttttcaa cactaactgt 138660 acctctaaat aagcttcctt caaattgtgt tgtaacatga taagtaaatg tttttcttag 138720 gtcccaggca gtttgtattg ataattcaac tatatactta ttcttatctt ctaattctac 138780 tacattttgc catctaattt caatatttga aggttcttga ccgataatcc aatcacttga 138840 atcaataaag agttcttttt cgtctccgtt agctctataa atatcaattc tataagcttt 138900 aagataatta gccattgtat tatttctaat attttcttgt atattagaac caacaaattc 138960 aaatattgga gaaaaaatat tgttaccatc aaattgtaat tcataaattg cttcacttac 139020 aggagaaatt aaagaaatag tagaccactc agataatccc ttagaaatta aatttcctcc 139080 aaagtatgtg gttttatcaa tatttacata ctgttgtaca tcactagagt aagtttgaat 139140 atgtcctctt ccatttggct tagtagaaga aagccagtct gttgttaaca taatttgcat 139200 tttatatcta acatcttttt ctgcgccaga catagtaaaa gcttcttgct ttatatcaat 139260 gaaatattct ccattagcat ttttttgtac ataaggcatt gtagtattat atccagttgg 139320 ctgaattata ttttttgaag gatcttttaa atcaatatat tcagcaaatg gatttctaaa 139380 aggaataaaa tcatctaggt atccttctcc aactggtaat agcgcacttt tttctgtctc 139440 agcatttctg attctaataa atcctccttt aaaatcagaa attttgttac caactgctgg 139500 ttcaaaaaaa agtctaaacg tgtcagttgg ttttcctaat tcaaaggcag gatgcgtaat 139560 aggaactaat ggtggatata aaatattata ataaatagcc atttagttct ttcctccttt 139620 ttctctttta ttcttcttca ctaatagccc aatctaagat attaaatttg ccatccatat 139680 cattttgagc aactaatgtt tttccaatat atggattttt aatataaact tttaatgttg 139740 gctttgcgat actaatagaa ttattagctt ttccataagt aaccaatgct gcttcgccag 139800 agtcgtttag ccattctcta ataaagttag tatctaaact atttgtccat gtaatatttt 139860 ggaaagtatt agcagtataa tcttcttttt tatttgcttc tcgcactggt tgtactacat 139920 taccgttcat ataagcttgc gttaatgcac ttgttgtacc ttccattggt tgtgataata 139980 cttcaatagt aaagtttgaa atagtatttt ctaaactttt agaagtagcg aataattcct 140040 gcaattgatg acgaaccgca aatcgtacaa atgcttgtgg aatattatca tcttctgcag 140100 caatagtttc ttcttggaca ccattaacta tatttgtaaa tacatcttct ggtacgtcca 140160 ccaaattttt gccttcgtta aagactaaaa cagtttttct ggagttatca aaagcaatat 140220 gagttggtgc taattcaact aatacatctg gtactcctgg ttgaaccttc catttatctc 140280 ccttaacata agttagtggt tgacctgtat taatagattg ttgtgcgaat ttaagtttta 140340 aattatctgg atctgtatca tcaatttgta aagcataaga taaacctgaa gttgtagaag 140400 ttacaactgg tggataactt aagttttcaa ttaattgtga aatataagga actaaacgtg 140460 atagctcatc tgtagttaaa acaatgtcct cgaaattatt tggtacttta tcaattaatt 140520 gggttaatgc aacctcttgt gtagctaatg cacgaatagc ctctgcatta gtaattaaca 140580 tatcaacaat atcgattttg ccaccaaatc tagtcaaaat ttgtttttct gaattatcta 140640 caaaacgtct atctggtgtt tctcttacca tttctgagac tgtctcagga agtataatat 140700 tcccttgatg gtcacgaaaa gtgtgttttt taaatccttc cattatcctt ttcctccttt 140760 tgttttttgc tctaaaagta ttgaaaaaga ggataagcac attgttattg cataccctct 140820 aattcttttt atatattacc caattgaatc aattggaaat gttattagtt gtcctttagt 140880 acttatttta atcttataat atccttgata ggttttaatt cttactgaat tttctccctc 140940 ttcgtcagtt tttgttgtat acaattgacg gataccatct tcatggtata ctcggaccaa 141000 ccaattctgt ccttttgggt ctcttaaaat taaagattta ctttggctca cctaacatac 141060 ctccttctgg agtttttgga agaatagctt tagtttgttc gtaatcttct ggattattca 141120 taaattttgt aatatctaca tcattatcta atacttctaa taatgtaata attccagttc 141180 ttcctccaaa attatattga tctccttgat ttgtaaatat caattctaat acatttccat 141240 ctgaatcatt taatttattt gataccacaa ctgcttttct attatattta gttgctgatg 141300 gaatttctat attaattact gaaccaatat ccaataaatc tggatgagca actacagcta 141360 atccattttt aattggcaaa ctattctcta ctgtaataaa gtcttctggt tcataccgtc 141420 ctcttacagt agttgaagtt ggaatgccct gttcaaaatt aatatcttct acaaattctt 141480 cagataaatc aatattcaaa gtcgcatatg tagaattagc tacagcccaa tgattttttt 141540 ctaaatccac ttgagcaaac ccattctcta cagcgtaaat attcatactt gttttttcag 141600 gaagaattct tggcgttcct ttctctattg ctggttctgc tacataagta ggagcagaat 141660 atacttttgt aggtaattta gttgaaatag ccccaatagg agaggatggg gtggataatc 141720 gtgaaactgt tatcgcgcca ctactcttag gcacccataa ttcattagaa gctaattgta 141780 aactagtttt accaatttct tgtactctat aaacttcatt ggaagatagt cttctttgag 141840 atactccacc gacatcagcg ttggttgcaa caactggttt tttaacattt acaacatatg 141900 ttcctttagg atcaattgta ggttttgaag gtttttctcc tgctgattgg gattggttat 141960 aatctgcatc agaaaaaatt cgcttcacac cataga 141996

Claims (10)

  1. 엔테로코커스 패칼리스(Enterococcus faecalis)에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어지며, 수탁번호 KFCC11693P인, 박테리오파지 EF5.
  2. 삭제
  3. 삭제
  4. 제1항의 박테리오파지 EF5를 포함하는, 항생용 조성물.
  5. 제1항의 박테리오파지 EF5를 포함하는, 사료 첨가용 조성물.
  6. 제5항의 사료 첨가용 조성물을 포함하는, 사료.
  7. 제1항의 박테리오파지 EF5를 포함하는, 소독제.
  8. 제1항의 박테리오파지 EF5를 포함하는, 세척제.
  9. 제1항의 박테리오파지 EF5를 인간을 제외한 개체에 투여하는 단계를 포함하는, 엔테로코커스 패칼리스(Enterococcus faecalis)에 의한 감염성 질병의 예방 또는 치료 방법.
  10. 제9항에 있어서,
    상기 엔테로코커스 패칼리스(Enterococcus faecalis)에 의한 감염성 질병은 요로감염, 창상감염, 균혈증, 심내막염, 수막염, 혈류감염, 장관계감염, 치주염, 치은염, 농양, 균혈증 및 패혈증으로 이루어진 군에서 선택되는 1 이상인 것인, 엔테로코커스 패칼리스(Enterococcus faecalis)에 의한 감염성 질병의 예방 또는 치료 방법.
KR1020170169631A 2016-12-23 2017-12-11 신규한 장알균 특이 박테리오파지 ef5 및 이를 포함하는 항균 조성물 KR102066898B1 (ko)

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Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GenBank: KY549443.1, Enterococcus phage EFP01, complete genome, 2017.09.30.

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