KR20180074578A - 신규한 장알균 특이 박테리오파지 ef1 및 이를 포함하는 항균 조성물 - Google Patents

신규한 장알균 특이 박테리오파지 ef1 및 이를 포함하는 항균 조성물 Download PDF

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KR20180074578A
KR20180074578A KR1020170169607A KR20170169607A KR20180074578A KR 20180074578 A KR20180074578 A KR 20180074578A KR 1020170169607 A KR1020170169607 A KR 1020170169607A KR 20170169607 A KR20170169607 A KR 20170169607A KR 20180074578 A KR20180074578 A KR 20180074578A
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연영은
김영주
한범구
김현일
박철세
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Abstract

본 발명은 장알균, 특히, 엔테로코커스 패칼리스(Enterococcus faecalis)에 특이적인 사멸능을 갖는 신규 박테리오파지 EF1, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 장알균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다. 본 발명의 박테리오파지 EF1을 이용한 장알균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 장알균, 특히, 엔테로코커스 패칼리스에 대한 특이성이 매우 높고, 상기 박테리오파지 EF1은 상기 장알균에 감염하여 장알균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 EF1은 사람, 동물, 식물 등 세균외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 EF1은 장알균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.

Description

신규한 장알균 특이 박테리오파지 EF1 및 이를 포함하는 항균 조성물{Novel Enterococcus faecalis specific bacteriophage EF1 and antibacterial composition comprising the same}
본 발명은 장알균, 특히, 엔테로코커스 패칼리스(Enterococcus faecalis)에 특이적인 사멸능을 갖는 신규 박테리오파지 EF1, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 장알균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.
일반적으로 엔테로코커스(Enterococcus)속 균주는 장알균이라고 부르며 그람양성이다. 대표종은 엔테로코커스 패칼리스(Enterococcus faecalis)와 엔테로코커스 패시움(Enterococcus faecium)이 있다. 상기 균주는 사람이나 온혈 동물의 장관 내에서 상재하며 분변을 통해 배출된다. 일반적으로 병원성은 비교적 낮지만 드물게 아급성 심내막염이나 요로감염의 원인이 된다거나, 치주감염 장관계 감염, 창상감염 및 패혈증 등 원내감염의 주요 원인균으로 알려져 있다. 엔테로코커스 패칼리스 감염의 특징 중 하나는 치아 뿌리, 요도뇨관, 요도 스텐트, 심장 판막 등에 바이오필름을 형성한다는 것이다. 바이오필름은 미생물에 의해 생성된 막 형태의 구조물로, 바이오필름으로 증식하는 미생물은 동일 미생물의 플랑크톤 형태보다 항생제와 숙주방어에 대한 저항성이 높다. 이와 같은 바이오필름 감염을 해소하는데 많은 어려움이 있으며, 만성 또는 재발성 감염이 흔히 일어난다. 또한 엔테로코커스 속 균주는 드물게는 감염형 식중독을 일으키기도 하는데 식품이나 공공용수 등의 오염지표로서 사용되는 세균이다. 특히 반코마이신에 내성을 나타내는 장알균은 다른 병원성 세균에 비해 상대적으로 연구가 많이 되지 않고, 병원성에 대한 중요도가 낮았으나 최근 다양한 장관계, 피부, 호흡기 감염에 있어 원인세균 또는 복합 감염의 원인으로 주목받고 있다. 이와 관련하여, 엔테로코커스 패칼리스는 반코마이신에 강력한 항생제 내성기전을 가지는 것으로 알려졌으며, 반코마이신 내성 장알균(VRE)은 1986년 처음 보고되었는데, 병원감염의 중요한 원인균으로서 치료가 어려워 감염 관리의 주요 대상 세균이다. 전에 반코마이신을 투여 받은 적이 있거나 다른 항생제라도 오랫동안 사용한 사람은 이 균에 감염될 수 있다. 또한, 반코마이신 내성 유전자가 황색포도상구균 같은 다른 그람 양성 균으로 전이될 가능성 때문에 더욱 위험하며, 특히 내성 유전자인 vanA가 이동성 전이인자에 의해 빠르게 전이됨으로써 퍼져나가는 것으로 알려졌다. 최근에는 vanB, vanD, vanE, vanG 같은 많은 내성 유전자가 발견되었으며 이 중 vanB는 vanA처럼 플라스미드에 존재하여 빠르게 전이될 수 있는 내성 유전자다. 이러한 항생제에 대한 내성은 엔테로코커스 패칼리스의 바이오필름 형성능으로 인하여 더욱 강화되고 이에 따라 균의 제거가 어려워지면서 효과적인 치료제 개발이 요구되고 있다.
한편, 박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다. 따라서 최근 세균성 질환의 대처 방안으로 박테리오파지(bacteriophage)의 활용이 크게 주목을 받고 있다. 특히 2000년 이후에 항생제 내성균의 증가로 인하여 기존 항생제의 한계성이 나타나고, 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다.
그러나 현재까지 장알균에 의한 감염증을 예방 또는 치료하기 위한 신규한 박테리오파지에 대한 연구는 여전히 부족한 실정이며, 장알균에 특이적인 용균활성을 갖는 박테리오파지 및 이의 응용에 대한 기술 개발이 필요하다.
이에 본 발명자들은 장알균에 특이적인 용균활성을 갖는 박테리오파지를 분리하고 분리된 박테리오파지의 형태적 및 유전적 특성을 분석하여 상기 박테리오파지가 장알균, 특히, 엔테로코커스 패칼리스를 선택적으로 사멸시킬 수 있음을 확인함으로써 본 발명을 완성하였다.
본 발명의 목적은 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF1을 제공하는 것이다.
본 발명의 다른 목적은 상기 박테리오파지 EF1을 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 EF1을 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.
상기 목적을 달성하기 위하여, 본 발명은 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF1을 제공한다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 항생용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 사료 첨가용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 소독제를 제공한다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 세척제를 제공한다.
또한, 본 발명은 상기 박테리오파지 EF1을 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 EF1을 이용한 장알균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 장알균, 특히, 엔테로코커스 패칼리스에 대한 특이성이 매우 높고, 상기 박테리오파지 EF1은 상기 장알균에 감염하여 장알균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 EF1은 사람, 동물, 식물 등 세균외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 EF1은 장알균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
도 1은 박테리오파지 EF1의 엔테로코커스 패칼리스에 대한 용균반 형성 활성을 확인한 결과를 나타낸 도이다.
도 2는 박테리오파지 EF1의 전체 유전자를 분석한 결과를 나타낸 도이다.
도 3은 전자 현미경을 통해 박테리오파지 EF1의 형태학적 특성을 확인한 결과를 나타낸 도이다.
도 4는 박테리오파지 EF1의 단백질 패턴을 SDS-PAGE를 통해 확인한 결과를 나타낸 도이다.
도 5는 박테리오파지 EF1의 pH 안정성을 확인한 결과를 나타낸 도이다.
도 6은 박테리오파지 EF1의 열 안정성을 확인한 결과를 나타낸 도이다.
이하, 본 발명에 대해 상세히 설명한다.
본 발명은 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF1을 제공한다.
본 발명에서, 용어 박테리오파지(bacteriophage)는 특정 세균에 감염하여 해당 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.
본 발명에서, 상기 장알균은 바람직하게는 엔테로코커스 패칼리스(Enterococcus faecalis)이다.
상기 엔테로코커스 패칼리스는 항생제에 내성을 갖는 다제내성균일 수 있으며, 예를 들어, 반코마이신, 아미노글리코사이드, 아즈트레오남, 세팔로스포린, 옥사실린, 설파메타졸 등 널리 사용되는 항생제에 대해 내성을 갖는다.
본 발명의 박테리오파지 EF1은 정이십면체의 머리를 가지며 긴 꼬리가 있는 사이포비리대(Siphoviridae)에 속한다. 보다 구체적으로 박테리오파지 EF1은 머리 부분이 약 100 nm의 크기이며 꼬리의 길이는 약 400-500 nm이다.
상기 박테리오파지 EF1의 단백질 패턴 분석 결과 55 kDa, 48 kDa, 40 kDa, 35 kDa, 31 kDa, 그리고 24 kDa 크기의 단백질을 포함함을 확인하였으며, 박테리오파지 EF1은 열 및 pH에 대한 안정성이 우수한 특성을 가지고 있다.
본 발명의 박테리오파지 EF1은 서열번호 1로 표시되는 염기서열을 전체 유전자의 전체 또는 일부로서 포함할 수 있다. 또한, 본 발명의 박테리오파지 EF1은 서열번호 1로 표시되는 염기서열, 및 상기 염기서열의 기능적 동등물로 이루어질 수 있다. 상기 기능적 동등물이란 염기서열의 변형, 치환의 결과, 상기 서열번호 1로 표시되는 염기서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 염기서열과 실질적으로 동질의 생리활성을 나타내는 서열을 의미한다.
상기와 같은 장알균 특이적 용균활성, 내산성, 내열성은 본 발명의 박테리오파지 EF1을 장알균에 의한 감염성 질병의 예방 및 치료용 조성물, 및 상기 박테리오파지 EF1을 유효성분으로 포함하는 다양한 제품에 적용함에 있어, 다양한 온도 및 pH 범위의 적용이 가능하게 한다.
본 발명의 박테리오파지 EF1은 도축장, 도계장 및 농장 부근의 하수로부터 시료를 채취하여 분리한 박테리오파지로서, 본 발명자는 이를 박테리오파지 EF1로 명명하고 2016년 9월 30일에 한국미생물보존센터에 수탁번호 KFCC11692P로 기탁하였다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물을 제공한다.
본 발명의 박테리오파지 EF1은 장알균에 특이적인 사멸능을 가지므로, 장알균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 사용될 수 있다. 보다 구체적으로, 본 발명의 박테리오파지 EF1은 엔테로코커스 패칼리스에 대한 특이적인 사멸능을 가지므로, 상기 균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 사용될 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서, 장알균에 의한 감염성 질병은 요로감염, 창상감염, 균혈증, 심내막염, 수막염, 혈류감염, 장관계감염, 치주염, 치은염, 농양, 균혈증 및 패혈증 등을 포함하나, 이에 제한되는 것은 아니며, 장알균에 의한 2차 감염을 포함한다.
본 발명의 약학적 조성물은 1 × 103 내지 1 × 1010 PFU/mL의 박테리오파지를 포함하며, 바람직하게는 1 × 106 내지 1 × 109 PFU/mL의 박테리오파지를 포함한다. 본 발명에 사용된 용어, PFU (plaque forming unit)는 박테리오파지가 플라크를 형성하는 것을 수치화한 단위이다.
본 발명의 용어 예방이란 조성물의 투여로 질병을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.
본 발명의 용어 치료란 조성물의 투여로 상기 질병의 증세가 호전되거나 상기 질병의 억제 또는 경감 및 이롭게 변경되는 모든 행위를 의미한다.
본 발명의 약학적 조성물은 약학적으로 허용 가능한 담체를 추가로 포함할 수 있다.
본 발명에서 용어, 약학적으로 허용 가능한 담체란 생물체를 자극하지 않고 투여 화합물의 생물학적 활성 및 특성을 저해하지 않는 담체 또는 희석제를 의미한다. 액상 용액으로 제제화되는 조성물에 있어서 허용되는 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로오스 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.
본 발명의 약학적 조성물은 질환 부위에의 도포 또는 분무하는 방법으로 이용할 수 있으며, 그 밖에 비강 분무, 경구 투여 또는 비경구 투여를 통해 투여할 수도 있으며, 비경구 투여의 경우 정맥 내 투여, 복강 내 투여, 근육 내 투여, 피하 투여 또는 국부 투여를 이용하여 투여할 수 도 있다.
상기 비강 분무의 경우 일정량의 박테리오파지 EF1을 물에 희석하여 분무기나 분무 시스템을 통해 비강으로 흡수 되도록 할 수 있다. 상기 비강 분무를 위한 비강 분무제 또는 호흡기용 제형으로는 에어로졸제 등을 들 수 있다.
상기 비강 분무제 또는 호흡기용 제형은, 수분산된 농축물 또는 습윤 분말을 분산시키기 위한 추진제 및/또는 기타 첨가제를 추가로 포함할 수 있다.
본 발명의 약학적 조성물의 적합한 도포, 분무 및 투여량은 제제화 방법, 투여 방식, 대상이 되는 동물 및 환자의 연령, 체중, 성, 질병 증상의 정도, 음식, 투여시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사나 수의사는 목적하는 치료에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다.
본 발명의 약학적 조성물을 유효성분으로 포함하는 경구 투여용 제형으로는, 예를 들어 정제, 트로키제, 로렌지, 수용성 또는 유성현탁액, 조제분말 또는 과립, 에멀젼, 하드 또는 소프트 캡슐, 시럽 또는 엘릭시르제로 제제화할 수 있다. 정제 및 캡슐 등의 제형으로 제제화하기 위해, 락토오스, 사카로오스, 솔비톨, 만니톨, 전분, 아밀로펙틴, 셀룰로오스 또는 젤라틴과 같은 결합제, 디칼슘 포스페이트와 같은 부형제, 옥수수 전분 또는 고구마 전분과 같은 붕괴제, 스테아르산 마그네슘, 스테아르산 칼슘, 스테아릴푸마르산 나트륨 또는 폴리에틸렌글리콜 왁스와 같은 윤활유를 포함할 수 있으며, 캡슐 제형의 경우 상기 언급한 물질 외에도 지방유와 같은 액체 담체를 더 함유할 수 있다.
본 발명의 약학적 조성물을 유효성분으로 포함하는 비경구 투여용 제형으로는, 피하주사, 정맥주사 또는 근육 내 주사 등의 주사용 형태, 좌제 주입방식 또는 호흡기를 통하여 흡입이 가능하도록 하는 에어로졸제 등 스프레이용으로 제제화할 수 있다. 주사용 제형으로 제제화하기 위해서는 본 발명의 조성물을 안정제 또는 완충제와 함께 물에서 혼합하여 용액 또는 현탁액으로 제조하고, 이를 앰플 또는 바이알의 단위 투여용으로 제제화할 수 있다. 에어로졸제 등의 스프레이용으로 제형화하는 경우, 수분산된 농축물 또는 습윤 분말이 분산되도록 추진제 등이 첨가제와 함께 배합될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 항생용 조성물을 제공한다.
본 발명에서, 용어 항생용 조성물은 약제 형태로 개체에 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다.
본 발명의 박테리오파지 EF1은 기존 항생제에 비하여 장알균에 대한 특이성이 매우 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 사료 첨가용 조성물을 제공한다.
축산, 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제이다. 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞여 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있기 때문에 좀 더 자연 친화적이면서도 기존의 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 EF1을 이용할 수 있다.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가시켜 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조상태일 수 있으며, 바람직하게는 건조된 분말형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량%, 바람직하게는 0.1 내지 2 중량%의 성분비로 혼합될 수 있다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.
본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus sp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에 (Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.
본 발명의 박테리오파지 EF1을 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.
본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.
또한, 본 발명은 박테리오파지 EF1을 포함하는, 음용수 첨가제를 제공한다.
본 발명의 음용수 첨가제는 상기 박테리오파지 EF1 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 장알균의 숫자를 감소시킬 수 있는 효과가 있다.
본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 소독제를 제공한다.
장알균에 특이적 사멸능을 갖는 본 발명의 박테리오파지 EF1을 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양계장, 축사 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF1을 포함하는, 세척제를 제공한다.
본 발명의 박테리오파지 EF1은 장알균에 특이적 사멸능을 가지므로, 장알균에 노출되었거나 노출될 가능성이 있는 개체의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 EF1을 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 EF1은 장알균에 특이적인 사멸능을 가지므로, 장알균에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다. 보다 구체적으로, 본 발명의 박테리오파지 EF1은 엔테로코커스 패칼리스에 대한 특이적인 사멸능을 가지므로, 상기 균에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다.
본 발명에서, 장알균에 의한 감염성 질병은 요로감염, 창상감염, 균혈증, 심내막염, 수막염, 혈류감염, 장관계감염, 치주염, 치은염, 농양, 균혈증 및 패혈증 등을 포함하나, 이에 제한되는 것은 아니며, 장알균에 의한 2차 감염을 포함한다.
본 발명에서, 용어 "개체"는 장알균에 의한 감염성 질병이 발병될 수 있는 모든 개체를 의미하는 것으로 인간을 포함한 포유동물, 가축류 또는 가금류를 포함하나, 이에 제한되는 것은 아니다.
상기 용어 "가축류"는 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.
상기 용어 "가금류"는, 가축 중 조류에 속하는 동물을 총칭하여 이르는 개념으로, 예를 들어, 닭, 오리, 꿩, 메추리, 타조, 거위 또는 칠면조 등을 포함하나, 이에 제한되는 것은 아니다.
본 발명의 방법에서 상기 박테리오파지 EF1 또는 조성물은 약학적 제제의 형태로 개체에 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다.
본 발명의 방법에서 상기 박테리오파지 EF1 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있으며, 구체적으로, 구강, 직장, 국소, 정맥 내, 복강 내, 근육 내, 동맥 내, 경피, 비측 내, 흡입 등을 통해 통상적인 방식으로 투여될 수 있다.
본 발명의 방법에 투여되는 상기 박테리오파지 EF1의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.
본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술분야에서 통상적으로 사용되는 의미를 갖는 것이다.
이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.
실시예 1: 신규한 박테리오파지의 분리 및 배양
1.1 박테리오파지의 스크리닝 및 분리
장알균에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 용균반 분석을 수행하였다. 시료는 박테리오파지가 존재할 것으로 예상되는 도축장, 도계장 및 농장 부근의 하수로부터 채취하였으며, 박테리오파지 분리에 사용된 반코마이신 내성 엔테로코커스 패칼리스는 아시아태평양감염재단으로부터 분양받은 것을 사용하였다.
구체적으로, 채취한 시료를 균질화 한 후 엔테로코커스 패칼리스와 함께 35℃에서 하룻밤 동안 진탕배양 하였다. 상기 배양액을 4,000 rpm으로 20분간 원심분리한 후, 상등액을 공극(pore) 크기가 0.45 μm인 필터를 이용하여 여과하여 시료 여과액을 얻었다.
전배양된 엔테로코커스 패칼리스 150 μL를 취해서 멸균된 튜브에 넣고 3 mL의 BHI(Brain Heart Infusion) 탑 아가(top agar)((Brain infusion solids 12.5 g, Beef heart infusion solids 5.0 g, Proteose peptone 10.0 g, Glucose 2.0 g, Sodium chloride 5.0 g, Disodium phosphate 2.5 g/L) +0.6% agar)를 첨가하여 잘 혼합한 후 미리 만들어 둔 2% BHI 아가 플레이트에 중층하였다. 실온에서 30분간 방치한 후 상기 시료 여과액을 각각 10 μL씩 점적하고, 다시 실온에서 30분간 방치한 후 35℃에서 24시간 동안 배양하여 박테리오파지의 유무를 확인하였다.
박테리오파지의 존재를 확인한 시료로부터 박테리오파지를 순수 분리하기 위해서 소프트 아가 오버레이(soft agar overlay)법을 이용하였다. 박테리오파지가 존재하는 것으로 확인된 시료를 식염수로 적절히 희석하여 각 희석단의 시료 희석액 100 μL를 취하고, 대상 세균(엔테로코커스 패칼리스) 배양액 150 μL에 혼합한 후 0.6% BHI 탑 아가 3 mL을 첨가하여 잘 혼합한 뒤, 미리 만들어 둔 2% BHI 아가 플레이트에 중층하여 방치한 후 35℃에서 24시간 배양하였다. 배양한 각 플레이트에서 형성된 용균반을 확인한 후, 이를 선택하여 400 μL의 SM 완충액 (NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1 M Tris-HCl (pH 7.5) 50 mL)에 현탁하고, 실온에서 4시간동안 방치하였다. 상기 박테리오파지액 100 μL를 0.6% BHI 탑 아가가 포함된 세균 배양액 500 μL와 혼합한 뒤 150 mm 지름의 BHI 플레이트에 분주하여 중층한 후 완전히 용균이 일어날 때까지 35℃에서 배양하였다. 배양이 종료된 후, BHI 플레이트에 15 mL의 SM 완충액을 가하고 실온에서 4시간 동안 천천히 교반하면서 박테리오파지 용액을 회수한 후 4,000 rpm으로 20분간 원심분리하여 상등액만 취하고 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 용액을 회수하였다. 이렇게 얻어진 최종 박테리오파지의 용균반을 확인한 결과를 도 1에 나타내었다.
도 1에 나타낸 바와 같이, 2-3 mm의 크기를 가지는 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 엔테로코커스 패칼리스에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.
1.2 박테리오파지의 대량 배양 및 정제
상기 실시예　1.1에서 확인한 엔테로코커스 패칼리스 특이적 박테리오파지를 대량 배양하기 위하여, 1.1에서 회수한 최종 박테리오파지 배양액을 1.0×108 PFU/mL로 희석한 후 전배양된 엔테로코커스 패칼리스 1.0×109 CFU/mL과 혼합하여 MOI (Multiplicity of infection)가 0.1이 되도록 하여 35℃에서 120 rpm으로 9시간 동안 배양하였다. 배양이 종료된 후 배양액 전량을 6,000 rpm으로 30분간 원심분리하여 상등액을 수득하였고, 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 배양액을 회수하였다. 분리된 박테리오파지를 박테리오파지 EF1(Bacteriophage EF1)로 명명하고, 2016년 9월 30일 한국미생물보존센터 (Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하고 수탁번호 KFCC11692P를 부여받았다.
실시예 2: 박테리오파지 EF1의 전체 유전자 분석
박테리오파지 EF1의 전체 유전자를 분석하기 위해, 박테리오파지 EF1 배양액에 20 mM EDTA, 50 μg/mL의 프로테나아제 K, 0.5% (w/v) SDS를 가한 후, 50℃에서 1시간 동안 정치하였다. 동량의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합한 후 실온에서 12,000 rpm으로 10분간 원심분리하여 상등액을 회수하였다. 회수된 상등액을 동일 부피의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합하여 실온에서 12,000 rpm으로 10분간 원심분리한 후 상등액을 회수하고, 상기 상등액에 3M 소듐 아세테이트를 전체 부피의 10%(v/v)가 되도록 가한 후 혼합하였다. 여기에 2배 부피의 차가운 95% 에탄올을 가하여 혼합한 뒤 -20℃에서 1시간 동안 정치하였다. 반응이 끝난 후 0℃에서 10분간 12,000 rpm으로 원심분리하여 침전물을 획득하였고, 차가운 70% 에탄올로 2회 세척한 뒤 에탄올을 완전히 제거하여 DNA 펠렛을 회수하였다. 회수한 펠렛을 최종 부피가 50 μL가 되도록 TE 완충액 (Tris-EDTA, pH 8.0)에 용해시킨 후 DNA 농도를 측정하였다. 전체 유전자 분석을 위해 PacBioRSII 를 이용하여 유전자 시퀀싱을 시행하였다 (마크로젠, 한국). 시퀀싱 결과로 얻은 박테리오파지 EF1의 유전자 맵은 도 2에 나타내었다.
도 2에 나타낸 바와 같이, 박테리오파지 EF1의 전체 유전자의 크기는 141,996 bp 이며, GC함량이 32%, 전체 ORFs의 수는 172개임을 확인하였다. 분석한 박테리오파지 EF1의 전체 유전자 서열을 서열번호 1로 나타내었다.
실시예 3: 박테리오파지 EF1의 형태학적 특성
박테리오파지 EF1의 형태학적 특성을 알아보기 위해, 배양액을 0.01% 젤라틴 용액에 희석하여 2.5% 글루타르알데하이드(glutaraldehyde) 용액으로 고정하였다. 이를 carbon-coated mica plate(ca. 2.5 x 2.5 mm)에 적가한 후, 10분간 적응시켜 멸균증류수로 세척하였다. 이를 구리 격자(copper grid)에 끼워 2% 우라닐 아세테이트(Uranyl acetate)에서 30-60초간 염색하고 건조시킨 후, 투과전자현미경 (Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000~400,000)으로 관찰하였다. 그 결과를 도 3에 나타내었다.
도 3에 나타낸 바와 같이, 박테리오파지 EF1은 정이십면체의 머리를 가지며 긴 꼬리가 있으며, 보다 구체적으로 박테리오파지 EF1은 머리 부분이 약 100 nm의 크기이며 꼬리의 길이는 약 400-500 nm인 사이포비리대(Siphoviridae)에 속함을 확인하였다.
실시예 4: 박테리오파지 EF1의 단백질 패턴 분석
박테리오파지 EF1의 단백질 패턴을 분석하기 위해, 1.0×1010 PFU/mL로 준비된 박테리오파지 EF1 40 μL를 10 μL의 5X 로딩 완충액과 혼합하고, 10분간 끓인 다음 12.5% SDS-PAGE를 수행하였다. SDS-PAGE 수행 후 단백질 전기영동 결과를 도 4에 나타내었다.
도 4에 나타낸 바와 같이, 박테리오파지 EF1은 55 kDa, 48 kDa, 40 kDa, 35 kDa, 31 kDa, 그리고 24 kDa 크기의 주요 단백질을 가지는 것을 확인하였다.
실시예 5: 박테리오파지 EF1의 다양한 세균에 대한 감염성 조사
박테리오파지 EF1의 다양한 세균에 대한 감염성을 조사하였다.
우선, 박테리오파지 EF1의 엔테로코커스 패칼리스에 대한 용균 활성을 확인하기 위하여, ㈜옵티팜에서 분리하여 보유하고 있는 엔테로코커스 패칼리스 균주를 대상으로 소프트 아가 오버레이법을 수행하였다. 그 결과를 표 1에 나타내었다.
대상 세균명 대상 세균수 용균 활성이
확인된 수		 용균 활성이
확인되지 않은 수		 감수성 비율
Enterococcus faecalis 12 균주 12 균주 0 균주 100%
상기 표 1에 나타낸 바와 같이, 본 발명의 박테리오파지 EF1은 엔테로코커스 패칼리스 12 균주 모두에 대해 감수성이 있는 것을 확인하였으며, 이를 통해 엔테로코커스 패칼리스에 대한 용균활성이 탁월함을 확인하였다.
다음으로, 박테리오파지 EF1이 다른 세균에 대해서 용균활성을 나타내는지 확인하기 위하여 ㈜옵티팜에서 분리하여 보유하고 있는 균주와 미생물 자원센터 등에서 분양받은 균주를 대상으로 소프트 아가 오버레이법을 통해서 감염성을 확인하였다. 그 결과를 표 2에 나타내었다.
Figure pat00001
표 2에 나타낸 바와 같이, 본 발명의 박테리오파지 EF1은 대장균, 살모넬라 등의 다른 균주에 대해서는 용균활성이 없는 것을 확인하였다.
따라서, 본 발명의 박테리오파지 EF1은 엔테로코커스 패칼리스에 특이적으로 용균활성을 나타내는 박테리오파지임을 확인하였다.
실시예 6: 박테리오파지 EF1의 pH 안정성 조사
박테리오파지 EF1의 pH 안정성을 확인하기 위하여, 다양한 pH 범위 (pH3, 4, 5, 6, 7, 8, 9, 10)에서 박테리오파지의 수를 측정하였다. 소듐 아세테이트 완충액, 소듐 포스페이트 완충액, Tris-HCl을 이용하여 각각의 pH에 맞게 조제한 후 990 μL로 분주하여 준비하였다. 박테리오파지 EF1 배양액을 1.5×108 PFU/mL로 준비한 다음, 상기 배양액을 10 μL씩 취하여 pH에 맞게 조제한 각각의 완충액에 첨가하여 2시간 동안 상온에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의해 타이터(titer) 측정을 수행한 결과를 도 5에 나타내었다.
도 5에 나타낸 바와 같이, 본 발명의 박테리오파지 EF1은 pH4-10의 범위에서 2시간 동안 안정한 것을 확인하였다. 이를 통해 박테리오파지 EF1은 pH 안정성이 우수한 박테리오파지임을 알 수 있다.
실시예 7: 박테리오파지 EF1의 열 안정성 조사
박테리오파지 EF1의 열 안정성을 확인하기 위하여, 20℃, 30℃, 40℃, 50℃, 60℃, 및 70℃ 등 다양한 온도 범위에서 일정 시간 방치 후 박테리오파지의 수를 측정하였다. 보다 구체적으로, 박테리오파지 EF1 배양액을 2.0×107 PFU/mL가 되도록 희석한 후 배양액 5 mL을 멸균된 튜브에 분주하고 30분, 1시간 및 2시간 동안 각 온도에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의한 타이터 측정을 수행한 결과를 도 6에 나타내었다.
도 6에 나타낸 바와 같이, 본 발명의 박테리오파지 EF1은 20-70℃의 범위에서 약 30분 이상 안정한 것을 확인하였다. 이를 통해 박테리오파지 EF1은 내열성이 우수한 박테리오파지임을 알 수 있다.
상기 실험으로부터 박테리오파지 EF1은 장알균, 특히, 엔테로코커스 패칼리스(Enterococcus faecalis)에 대한 특이성이 매우 높고, 장알균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있음을 확인하였다. 또한 본 발명의 박테리오파지 EF1은 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 EF1은 장알균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
비록 본 발명이 상기에 언급된 바람직한 실시예로서 설명되었으나, 발명의 요지와 범위로부터 벗어남이 없이 다양한 수정이나 변형을 하는 것이 가능하다. 또한 첨부된 청구 범위는 본 발명의 요지에 속하는 이러한 수정이나 변형을 포함한다.
이하 본 발명의 박테리오파지 EF1을 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 본 발명을 한정하고자 함이 아니고 단지 이를 구체적으로 설명하고자 함이다.
제제예 1: 의약품의 제조
1.1 주사제의 제조
박테리오파지 EF1 1 × 106 PFU/mL
주사용 멸균 증류수 적량
pH 조절제 적량
통상의 주사제의 제조방법에 따라 1 앰플당(2 mL) 상기의 성분 함량으로 제조한다.
1.2 액제의 제조
박테리오파지 EF1 1 × 106 PFU/mL
설탕 20 g
이성화당 20 g
레몬향 적량
정제수를 가하여 전체 1,000 mL로 맞추었다. 통상의 액제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충전하고 멸균시켜 액제를 제조한다.
1.3 정제의 제조
박테리오파지 EF1 1 × 106 PFU/mL
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.
한국미생물보존센터(국내) KFCC11692P 20160930
SEQUENCE LISTING <110> Optipharm.CO.,LTD <120> Novel Enterococcus faecalis specific bacteriophage EF1 and antibacterial composition comprising the same <130> 1-35P-1 <150> 10-2016-0178129 <151> 2016-12-23 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 141996 <212> DNA <213> Bacteriophage EF1 <400> 1 cctgagttcc tgactcaata aaaattccta gttgatttgc taaatctttt aatttagttg 60 acaacataaa taatacctgg tcttcacgta atttcatacc atttgaaata gaagcaattt 120 ctgctaagaa tttcatagat gtatgaatat aatcaaaacg gaaatattta acatcatgtt 180 ctcgataata ttttctaata gtagcttcaa tttctgtaac agagaaatct gaaataattt 240 caaaatataa cggagcctca gacataattc tagctccttc tctcaatact ttttcttggt 300 cttcgtcaaa tttaccatct aaaattatat cttcaggaat tcctgtaaga aatgctaatg 360 ccattgtttg acattcttct gaatctaact ctgtagaaat aaataaagaa ccttctgctt 420 ctccattgtc aacccaagct tgtttatgtt ggtcataaat tacagggaag gcagagtaac 480 aagctcgaga catcataata cgagatttcc cattccctgt tggagctgaa tacaaataga 540 atttacctaa tcgatctcca cgagttatcg tatccatcat tgtaattgga gaaggtgaac 600 caaaatctgg ggcttcttta aaagaatcaa ttaaatttaa taatccttca ccagcttgga 660 tactttcaga agctacatta cttgcagaag tatccataat atgttgaata ttatctgaaa 720 tctcatttgc catatccttt aaagaagtat gttcaaacca attttgttga taagcaatca 780 attcttgatt ctctgtatcc caatcataaa actgttttac agatacgcca tttttctcaa 840 gagagcgaag tagagttaat tttttaattg tgtctagggc tggctcaaaa gctgttggat 900 ctcctacttg tgatagttta tcaaagtaat attcaccttg tcgagcatat tttccttcag 960 caatttctat ctcttcatca aataaagtat taatttgcgg acgaccttga atatagcttt 1020 ctattatacc taagctaaat tttggatgtc ccattgaata taaattatac ataattgcat 1080 aagaaacttt ttgtaagcta tccacaaaat cagcttcgtt caccgcagcc atcttaggat 1140 gatcgaataa tgttggttgt acatataaag ctcctaataa catattagca atgtctttac 1200 gataatactt catttattta cttcactcca ctcatatcaa tagtaattct tttagtctta 1260 tcttcttgta aatcagaaat aattgtatca aacaattttt ctaaatcatc tttgtctttt 1320 cctttaataa aaattaattc tgtttgattt gtgtctgcac tgatatcttt tacataaatc 1380 cgaaaacatt gcttaaacat ttttattttc ctcctctgat aataaagaat ctaaatcaat 1440 taatttttct agtcgtttag ttttttgtgg aggaacaaca acctcttttg tcttatattc 1500 tattttagta acctgttcta aatgttttag ccccttataa taatctcttg cctcattata 1560 aaaataagga agtaaagcta taccactttc aggggttaat cgtttctttt taatatcaat 1620 taaataataa agtgttccca agagaccaga ttcagtgtaa ttaaattttt cagtaaactc 1680 tttaatctgt ttttgaacga gaggataatt aaccttacca ggcgttaact gttctaataa 1740 atcagttata cctgctctct tggctctttc ttcctgcatt gtatcgtaac aagttttatg 1800 atagtatcct ttggaagtat gttcaaacag ttcttcttcc ctgctaaaat gttgtctaca 1860 atattgacaa ataacttttc tttgtttagc cattatatgt atcctcctta aaaaagaaaa 1920 gcgaggaagt tattcctcct cgctcatcaa attaatctta ttgttgtgaa gcagccaaat 1980 ctttcaagtt tgtaattgct agatctacta attcagcttg ttcaggtgta gcatcggcta 2040 ctttattatc atgacctaaa gcttcattta caattgtacg aatacgagga ccatagaaat 2100 cagcattagc ttccattaat tctccagcaa gattattgaa cgtttcaatt aattcttcga 2160 taggagtatc aattttattg tatttatgaa ctgtagaaga attatctgtt acagaatcaa 2220 ccccaaattg ttgttctaga tgagaaactg catcactaat tgctcctacc aaattatcat 2280 aattaaatac gatataatca ggagtactat caaaacgaga accagcgaca aaacgaggcg 2340 ttcctcgcat atatagacga gtttccaaac cttcttctgt atttactgct cgtgaataac 2400 caataatatc agccatacga gtaataattt tattaccacg tttatctaat gttggaataa 2460 tttggttata ttcttcgcca gattcatctt taaagacctt atcttgagaa tgagaaatca 2520 taactaatcc ataatcttca gccatgatag aacgtaattg ttcatcaaat tctttttcta 2580 ctaattgata tcctttacca taaggtaatt tcttttgatc tgcaatatct gacgcaccat 2640 gctggttaac aataaatttt tcacatagtc cgtatgcaat atctacagta tcaataataa 2700 tattatcata catttctttg gcttgatctg tttttaactg ccgtagaact tttttaaatt 2760 cagaccaact gttaattggc aatgctctta cgccaggaat tgcagaataa cctttttcaa 2820 aagccaatag caaagactta ggaaatcgtg ttgctgtagt agttttacct gatttaggtt 2880 ctccgtaaaa cattactgca tatcctttta aatctttaga aactttatga ggctcaacat 2940 taaaaatgtc aattgtgttt gccattgtat cactatcctt ttctcttttt tattttattt 3000 tattaatgtt tccaactaga aagagaaacc gaagcttctc ttcctaagta gagcattaat 3060 tagaattcaa aagtctttgc tctgtcaact tttggtgctg tatctttttt agatgtgtca 3120 gctgcaaatc cacttttacg ttgttgattg tctaaagccg caattgaaac ttcgtaagct 3180 ttacgcccat cttgaattgt tttaaataat tcatctgtaa tttcttttgg ttcaatagct 3240 gcaccagtaa ttacattttc tcgttttgta ctttcaaaga tttcaatacg agtatcacca 3300 aacgctgatt ctgtttcacg ttcttcatga attagattat taatcacttt accccatact 3360 tcaatcaaaa ctgggtttgt ttgacttgcg tcaagctgaa cgaaataatc gtaagcagct 3420 tcagtttcaa taataaattt agctgggaaa caaacgtcac gataatcaaa gatgtttgca 3480 ttcaatacat atcgtccagt aggctcatct ttataaattt cttctacagg ttgagctgtt 3540 aatagcaagt ctgttgtaaa ttctgcacga ggaacacctg tgtttcgatc gtttaagaag 3600 cttgctcgaa tttgagttgc ttcaattaaa tcaccagctt gatttttaaa agcacttgaa 3660 ctaatagaag ttgttgtcat ataatttttt ccaatgaatg cttctggatt ttgtaaccaa 3720 ttgctgatag ttgcgaactg ttgatttgta gaaccatctt tgttcaattc acgataaaaa 3780 ccttgaagat ttacagattg gtctccagta tttaaagtta atgatccaac cattgcattg 3840 tacggttcac cagagctttt atctttatac tctttaactg cgaaatcgaa atcctctaat 3900 gtacctgaaa tttttacttg atttttaaaa gttgccataa tttaatttcc tccaatattt 3960 gtttatcttt tatattatat taatattata acctatttaa ctaaaaaagt caaattttat 4020 ttttttggac cagactcttt ccatcgtttt aaagctactt gtaaacgatc attaatttca 4080 tactcttgat aatacctaac tcgtttacaa accataatat cgtttccttt atattctgct 4140 ccaaaaactg gtgaactatg tttaaaagat tctttgacta aatcttctaa atcttcacga 4200 ctaaatactt tataatattt atctaaagct tgcacaatgc ttgtataatc ttcccaagta 4260 atcccaccat ctggactaat ttgaactcct gtggtatgga cacctttctt gattgggaaa 4320 attccttcct ttttaaggaa cgcatttaag tttcgagaaa aacttttaaa aattttcttt 4380 tcttgctttt cattttttgt cataataaaa tcctccataa atacttttta atatttctta 4440 tttaacttta taattaatta tatcatgttg aacataaaaa atcaaatttt taattaggaa 4500 taacagcttt acctacaata gcttttccaa ccactgctgt tgttgcatct tctggagttc 4560 catcattaat tgttcgttca acatatctag ttaaatcaat atcttcactc atgactaaac 4620 catgttttgg acaatataga tctcctgatt tatcgtctaa tttgcgatac acatttctca 4680 taactctttc ttcatcataa caaattggac aaaaattgtc aagatttaaa tttactttca 4740 taataaaaga ttcctccttt ttcttgtctt tataatttaa aaaggagaaa tcatccatta 4800 ttattcgtcg tcctcaataa ctaattctag ttcactagga ctacaattac aatattgttg 4860 aaaaccatga tatttacaat ttaaagtttt ttctccatgt tcattttttt cagaatataa 4920 acgaattaat ttatcctttt cctgcttaca tactggacaa aaatatttcc tagaatagta 4980 tctaatcata ttactcacca aattctcgtt ttaacattaa attaatttgt tgaatattaa 5040 aaccagttgt taaaactgtt ccatcttctc gcattaatac tggcaatgat aatacattac 5100 ccttttctac taattcatta tatttctctg gatctttttc tttatgaaca cttacaatat 5160 gcttgttata tttttgcata tttttagttt ttaaataacc ttccaattga ttacaatatg 5220 gacaattatc ttttgatagt ttataaatca aattatctca ctactcctta ataaaattat 5280 taaacatttc taaatcttct ttatgctctt ctggaataaa agttaattca aacttaatct 5340 tagggatatc tttctctaaa ataatttgaa tattatctaa tgctccgcag aaatgagtat 5400 aaattgtaat tccactacca aataataaaa ctggtttgtt tattaataca tctctattag 5460 aaattacaaa agatttaata tttttaggaa tctttccatt actccatgtg taagttccta 5520 taattatttt atcatattct tccaaatttg tcaagtcatc ttttgtattt tttataataa 5580 aattaacatt tgggtaagcc tttactataa aatcaacaaa agtttttgta ttcccagata 5640 gactctcata aactacaagc actttaggtc caagagagag agattctaca tgagagggaa 5700 ttgccttggg cggagctaat tttactccac ccgtgcaaaa tgttttttcg ttattatcca 5760 tattaaaggt cgtcccagct atttttgtcg tctgttgctt tagaataatt tactacacga 5820 ttttcaaaga aatctgtttt tgtattattg atattatctg aatcgtaagt cttaatccaa 5880 atcattggat tagttaaagc ttcagtataa actttggtta agcccaagtt ttgcaacaga 5940 atattagcac gccactcaat atagtgttct aattcttcca tatctaaaac agcataatcc 6000 ttatatagat ctcgagctaa ttcctgttca gatttaacta ctttatcaaa gaacgcataa 6060 acccaattac taaaatcttc actctcttgg ggtgaaagat gtaattcaga cataatttgt 6120 cgtacaacta acgtttggaa ataactatgt tgtacttcat cacgctggat caactgaata 6180 atttgtcctg ttcccaacat ttttttgttt cggtttaata agtaaaatgg agtaaatcca 6240 tttgtaaatc taataccttc aagtgctgcc atagctgcta aggctttagc catatttagt 6300 tttgttggag tttccaaaaa tgtttcaaat gtatccaaaa tcatttcact agaaccaaga 6360 atatatttgt ctgttttagg gatttcaaat acttccttcg cttcttcttt cgtcataaaa 6420 gtggtagcaa tataagtata actttcatta tgaattgatt cttgagaagc aatataagcc 6480 ataatagctt tgatagcagg gtttttaata tacgctgcca attgaccatc aataacagta 6540 gaaatagaat ctagtaatac taattgagaa atagctcgtt tgtaaaaagt tttttcaatg 6600 tcgctcatat tatctcgcca atctctacta tcagtattta accctacttc ttgaggtcgt 6660 ttatattcac ataaagtcgc taattttatg cagttctctt atgaacttct atatgttacc 6720 atatagacta gactatatct taaatagagg cgttaccctc taagtctcta aatttcgatt 6780 taaaggattt atcatgtatg ccattttcgc atacactacc taccgcttgg ccctactcta 6840 ctcggttctc gctaattatc ctctcgacaa taagctaccc tttcgatagt cgttacaatg 6900 ctaaaggtgt ttccaagtat aacccccttt aattctagct atataactag gggatagacc 6960 atatctttca cctagtacac tatttggttc acttgatttt ttaatttcta atgcttgggc 7020 ttctgttatc ttagaatttc tatttttctc cccaatattt gtcataagtc catttgtata 7080 ggcatgataa atattttctg atggagatac ccattctaag ttttcagccc tattatcatt 7140 tgtttttcca tttatatgat taacctgttc tttattttct gggtttggaa caaaagcttc 7200 tgcgacaagt ctatgaacct tataatcttt tccagctaaa ttaactctta agtatccacc 7260 ctttttctgt ggtcttcctt ttaattcttt acctgttgtt tgattataaa ttttcccatt 7320 gtctgacaca gcatatgtgt gaccaaaacc attttttatt ccttttattt cttttatcat 7380 attttaatgg aaacaccttt agctatctcg gtattgtccc ataaggagtt ccaccgattt 7440 aatagagttt tacttggact acctatttaa tccaaaaatt ttgttttagt tctgaatata 7500 cgtcatacca tacaggattt tgttcgtccc aatataataa tccagaatgt tctccaaaaa 7560 ttgactcatt tgtattttct gcatctttat taaataatct aatatgtttt tctaacatta 7620 aaatcttctc ctttatacta ctctgaacga tttgtccatt catcaattga atctaaccat 7680 tcagaaaaat catttgtttt tcgattttca tcaacaacaa aacaatcatg aagatagcta 7740 ataacttcat cttcagtttt atatccttta ctttcagctt cctcagtaag gtcatataag 7800 ttttgatata agaccttacc atcttctgta aattttgctt tcatttttta caaatcctct 7860 acattaaaat gaatgttctt ttgtttcttg taagcatcta aataaatttc ttccttgtct 7920 ccatttaagg ttacttcata atacatccca tcagaaattg ctgtagacaa aagtaattta 7980 ctattttgta gtacctttgt actccagaca atatatacat cttctaaagt atagtctccc 8040 tctaagtttt cgttagaata atctaagacc aattttttgg ctgcttttac aaataattca 8100 tgtgtcattt attttcctct tttctttaac cttatatatt tattatatca taataaataa 8160 ggaatttcaa ataagaaatt ccttatttca aacttgttcc caataatatc cataggcaga 8220 tttaattttt ccagttgcag cacttctaat attaccacta ccttttttta aaaaatctgc 8280 agcttcttta ctatttttaa aaaataaaac ttctcctgtt tttttatggg ttgcttttaa 8340 ttttatactt cttttgtcag cagaaattct agatcccttt tcagaatttg cttttgatag 8400 tccattatta taagcatgtt gagtattctc agatatagtt gcccattcta ggttagaaac 8460 aacattatta agtttatttc catctttatg gttcacacta tatttatttt ctggattttc 8520 taaaaaagct attgctacta atctatgagc ataaaattgt tgcttctttc catccatata 8580 gagagtatgt tgtctatagc catggctatt aattccgcca gacaatattc tatttcctct 8640 ttttaatctg ccataggagg agatctcata gacctcccat cccttgacag gtaaccataa 8700 ttctttcatt cttttctcct taagagctgc aagcaacaca atcatctaca cgagaagcgt 8760 catgagaacg agtataataa gaagatttaa ttcctctaga ccaaacttcc atatgaagac 8820 gaagtaagtt tttaacctta atatcatcca agatatataa attaaatgaa gtagattgat 8880 ctacccattt ttgacgttct gcgttgtgga gaatagccca catatgaccc aaatctctct 8940 ctccttcaaa ctccattaac atagttggtt tataatagaa ccatgtttct aaatttagtt 9000 ctggagcaat gacaggtgta cgagaatctt tcttaccatc aaaaaagata gtgtcataaa 9060 ctgtgtctgc cgctgcagta gaaccagcta ataaagaagt agaacctgtt ggagcaactg 9120 ctcttaaata tccattccgc atacctttct tagctttgga ataaacctct gctcgataag 9180 catcctcaat atcatcttga caaactcctc gatgatttaa ccattctcct gtgttccatt 9240 gacttccttc aaacaaagga taacttcctt tttcttgggc taaatctgcc gacgcctcaa 9300 taatatttag catgattctt tcttctaacc gagcaattaa tttagttgct tcaactgaat 9360 caaaatggat tccttgttta gccaaaactg ctgcaatacc ttgttcacca attccaatag 9420 cacgataacg attatttgta tattgggctt gtaaaacagg taaattatta acagtaataa 9480 ctgcatcagt agcacgaact tgaatacgta aaacctgtct taatttttca aatgctttaa 9540 catcgtcgct agtttgcggt ccttcaatag aaagagtaaa atctttcaaa acattgttaa 9600 gaacaataga agataaatta catgtcacaa aatctccact ttgtttatga acaataattt 9660 cccctaattc attaatagtt tctttagtca taattgtagg agattgattt tgtacaattt 9720 cgctcttata tgttcgattg aagtcgttaa tttcaacccg ttctcttatg aactgctata 9780 tgttaccata tagaacagac tatatcatca ccctttttta aaagggggct accgtttcgt 9840 ttaaaggact tacccgatta ttactcggaa gcctagccac ttggccctac tctactccca 9900 caactcgtgc tcggtttcga tagtcgtttg gcatttagta taaagtctaa tataattagg 9960 aaagtttttt gagtttattc tataatgtat tgtcgtattt ccaataccta gaatcctaga 10020 agcttcattt actgaaggat attcaatccc attaattaaa attggcttac tatttgcttt 10080 tttaatgctg tcaatcattt tctgtgtttt aggtttacca tacattgggt ttcgacaacc 10140 agaagaatta atagatttca atttccttgt atattcttta ttcgggttgt tcgtaaaagt 10200 gtctcctcca attgaggttg cttttatatt ataaaattct ttacttttta cagcgtcgaa 10260 ttgcataata taatattctt ctacttctcg aagttcttct tcagaatcac attcatcaat 10320 aataatctta aagaagtttt cctttccata tttagaaatg gctcttttta gataaacccc 10380 acttcctaaa tatttctccc aattattttt tcttccataa acacattttc ctatatattt 10440 catattattt attttgttta ttgtaatata tataaaacca tatctcacaa tatctacctc 10500 ctcggtagac tttatacatt tagcacagga ttgtcttttc ttaaagagtt tccctgttta 10560 gatagctttt tcgataagga ttgctcctta aaggtgctag tttatcaaca caaattagag 10620 cagtatacca ttcctgcatg attgttagga ttatctctat ttgcagtatc tcgataaaac 10680 atataaggca aaccagtttc tagttgagaa cgcataattt ttttcataat ttcaatagct 10740 ggcaaacgtt tctttaaatg taaatcgtta ttatctacag ccttaaaata gtgataagag 10800 aaagcgtgac gatcaggatc tagcggtgat tttccatccc atttttcttt atcgtagaaa 10860 tcttccagag aaaaacctaa tacttttttc acttcgtgag ggtcaaataa ataccagtct 10920 cctcgttttt ctacttgttg cataaataaa tcaggaatag aagcacctaa aaatagttca 10980 tgcgctcgtt tagataggtc tcctgtattt aaacgcaact ctaagaaatc ttctatatca 11040 taatgccaaa catctaagta tgctgcaata gcaccactac gaactcctaa ttggtctact 11100 gaaacagcag tattatttaa ttgtttaagc caaccaacaa tacctcctgc agcaccaaca 11160 tttcctctaa tatctgaacc agcagcacga attttaccca cataaaccat gttattccac 11220 tatttctagt ggcatagact atatcttcat atatttgtta gatatatgct ttgcgctacc 11280 caattgggta tctcacccaa aaggtctcgt ttggttttcg tttggaaccc ttgattccaa 11340 gctatccaaa catttagtcg ttacacctta cttatatcta tatccagaaa atttttcact 11400 tttagaattg agtctataag agatagtttt tctatcaatt tttaattctc tcgcagcctc 11460 atttatgcta atatattcct tgccttctat taaaattgga atatttttag gactgcaagc 11520 tctcattttt ttcttccact cttctgtttt tggaactccc ttaaacgtct ccctagcttt 11580 ctcagccaac aagcgtttgg tttcttctat atgcttttta ccatagaagg ggttgctttc 11640 ccctttgtat ctaatagaag cttttgagcc aatctttaat ttagtttctt ctgaatgatg 11700 cttaccataa aagccattat cagaaccata atgaatatag tttccagggg acctcgagct 11760 tttagaaatg ttatattttt cctcctcgag attcaagtaa tattcttctc gttcaaaaac 11820 tttttcttta tcttctattt cttctaaaat agtaaaaaca aagttttctt tcccgtattt 11880 attaaaagca ttttgcagtt tagaatttat gtgtttgttc tgttccaatt ctcgaaagtg 11940 cgttgttttt cgtctttgag gattattagt tcgtccataa tattttatgc ccgtaaccaa 12000 attctctatc tggtatatat attctatttt tatcacatcc tttttaacaa aggatataga 12060 tataagtctt ggcacggtat tgccatatca tttctgactt aggtttcacc gttagcatcc 12120 tgttaaggac acaccctatt ttaggttcac aaagttttaa atcggcattt taaagtttac 12180 cgattcctcc cccagccttg cttaggcgag cagcatctgt attgtcatca tagatccctt 12240 gtaagctatc atctgttgtt aaaataaagc aactagaaaa ttgtgcatct ggtcgtccag 12300 catttgcaaa tgttggggta gccatagtaa cttcttgttt agatagtaca tcatataatt 12360 cttttacatg ttgtagtcta tcttgtgttt cttctcttaa taaagaaatc gcaattgtca 12420 tataacgttc ttgtggtaat tcaaatactg aacgtccttt atctttagct cgaataagat 12480 atctttcaga catattgtat aaaccagcgt aagtcaaaag taagtctcgt tctggcttaa 12540 tatatttacc taattcttga atttcttctt tagtatattt tacaaaaatt tctggttcaa 12600 taagaccttg ttcaccaaga gaagaaagaa gaccaaaata atcaccatat tttgaatcaa 12660 catcataact tcgatttttt gaagctcgtt tatacaacga atttaataga gccctagctg 12720 ctacaaattc aaattgtgta ttgcctaatt tatcaaagtc catcatttct ccatctttaa 12780 agtccatgat tctatcaatt gcattttgaa taataacatc attgatatct cggaaatcaa 12840 tctcttgtct accctggatt tgtttaataa cacccttctt atatgcttca aatgtatctg 12900 gatgaacgtc caatccttct ttaaagctat ctaatttatt aattaatcgt tgtggatcaa 12960 attccaattt tctcaatcca ttatctttta ctactactgt caaataaatc tctccttttt 13020 taattctgta atctttataa aacccaatat atagatattt atacggaata aaccacctta 13080 tatagaagca tatttaatca aggcaagtaa gacatctatc aatccattat tacaatcgat 13140 aaatactgat tcagcttttc cttctcgtcc tattacagaa acctcttttt tgtctatatc 13200 tacaatcata gctgtatatt tccctgtggt agaatataaa ataaacccac tagattttcc 13260 atcctcatca tgtttaatgt atatttcatc ttcattatat cctaaagcat ctatgatata 13320 aaaaactata gttctcatat ttgttcctgt tgtttcttta tcaaaatata agctcatgga 13380 tgaaataaaa tctctctcat aagagtgacg atcataaaac attaaattta aagaatcttt 13440 agttattata ggacttactt ctattcttcc ttttggagaa tatattttgc cctctaccct 13500 taaaacttta gagcggctaa tttcatttac aattgcactt acctcttcta atggtctcat 13560 atttttctcc ttttaaaatc attataaaat aaaaatcctt actatataaa gtataacata 13620 gtaaggattt tttgtcaaat tatttaattt tagtaccagc cattagccat ccaaaaagct 13680 tgtgccgctt cccatgaacc ataacgttga gctacataat tttctgcaac acgttcttgg 13740 tttgctggag aatgatctcc atttagataa gctgaatcta gttgataacg tccgatgtaa 13800 cgaccattcg ttgctgagta tgagccacct gattcacgtt gagcaatcca ttcttttgct 13860 gaatttgttc ctgttgaagg agcttgttcg acaataggtg attgttctac tactggagct 13920 tgttctacaa cgttttcttg cacaactgga gcttcaacta ccgctgctgt tgctggagcc 13980 tgaacctctc cattggttgg aatcaataat aaatctccag caaaaatcat attagcatca 14040 ctaactgtat caaggttaga ttgatggatc aaactatgat ctaatccata tttattaccg 14100 atagcagata aagtatctcc tgcttctact tcgtaataag tctgtccatc tttttgaatt 14160 tctgctgcgt gaacacctgt tccaccaata aataaagctc ctgctaatag tcctgcgata 14220 ataattttct tcaaattgtt attctccttt ttcttttagt ttttcgttaa gcaatgaagc 14280 atatccattt gcttcattaa attctaaaaa cttaattttg gcaacactta agccagtaaa 14340 acaatttcgt tgagaatcta ctaaagcttc tcgttcaaat tgtgctgaat taaagttgtc 14400 tttcacaagg tcgaaaaatg gaaagacgat atccccatct tctgaaaaag gaacgtctaa 14460 tagaaaagcc tcgtaaaccc aatcatctaa tgattccaat ttcttattta caactttata 14520 cgcctcttta ccaaaaatag tttttgttgc ttcaacgatg tattccatat gaaattcccc 14580 ttttcttgtt ttaataaaaa agaccctatt acaaaattat tacagttaaa ttacattaaa 14640 ataaccgtga ttttttcttt gtaattttta ctgaagtatg aactttatta tcatcttcta 14700 cagtaatttg attattttta actacaacaa cagggtaatg ttcttcaagt ttttctgcta 14760 atttttctac aaaaggattt agttttttag ccacaacaac ttctccttct gcaacttgag 14820 gacttttatg tcgagttact tttacttcat aaaatgtgtc gccatcttta ataccagcaa 14880 tacgtttttg agaatccaaa agtttaaatt caatatcttc gtcctcttgg agaaaaccga 14940 acgccatatt cgctaatccc catttgtctg cagtcatttg ttttgttgga gtagcacgac 15000 ctgcttcgat taaataattg tcccaatttt tcataatttc atattcaaat tcttgatgct 15060 taggatctga tttctttatg ccgtttttca aattttcttt aaattttcta cctgtaataa 15120 tagtattata atacatagac ataaattttc tatcctctgt agtttgttgg aatgcaccgt 15180 atttgttttc cataaaataa aaactccttt taaaattctt ttatttctta actatatata 15240 tatatattat catttttaaa taaatttttc aaattattaa attaaaaaag gctgaggaaa 15300 actcagtctt ttttaataga aattattctt caaccttttc ttcaatctct ttttcttcaa 15360 ttgttggggg agattgtagt tgttgaatag cttcaagcaa ttgtttattt gcagcttctg 15420 atttagccaa taaaacttct tgtcttgcta tttcacccat tgcttgagta agtaattctt 15480 gtgttccagt taattgttgt cgagcaacag ctaattcaaa ttctgcttgt tctaatagct 15540 gttcaaccgt tggatgttga cctgttttat tttccattat tgtgttacct ccactgatgt 15600 ttttccatct tctgaaattg caaaatgtcg aactttatta gtcatatctc cagtacgatt 15660 tgctctaata cgttcataca tagctgtttt tcggtcaatg aatgcttgga tttcattttc 15720 taattcagac ttcatttgtt ctaaggtttc aaaagtttca tgatccattt tgagatcccc 15780 taagttattt aggatatcaa tataagaacg taataaagca atacgagtac ctactttttc 15840 tgttgccata tctaaatcat ctggatgagt ttgactaact ccggtgcctt tgactatatt 15900 accatcaatc agttctgctg tagtaataac agtcacttta gatacgacat taataccatc 15960 tacttctaca aattctggaa aagaatcaga tgttataatt cgttctaata catttttaac 16020 ttccactaaa atttcctcct tatttaactt tataattaat tatatcatct ttaataaaaa 16080 aagtcaataa ttgtctcgcc aaacaccttt tatttttttt cgtgcttttt gtctagttaa 16140 tggataccat tgttcacttt tagctttttt taaaatttga gcttcaatgg gaatccatct 16200 accatcacta tcttttctat atattaacat attactctct ccctgtcaag taaattaaat 16260 tttaatccaa atatctcctt ctctacctgc tttacctgga tcagtaacac taaagaaaat 16320 tctaacccct ttaaatgata tatatgattg ttcagaagat aatgcagtat ctgttaataa 16380 ttcaccatcg ctcattctta aatagtgata atatccgctt tctgttggtt gtccactacc 16440 ggcgtaattt ctagggaata aaatagctgc atcacgacta ctagaataat ttctaaaaac 16500 aagctgtcca ataccattca ttgctccacc attaatatca atggctggtt tacgatttaa 16560 attacctaca taagttaaat taggttcaga aatttttagt gctggcatac ctaaataccg 16620 tttagtatct ccaatagtca tttgacccgc aggaatatga actcgtccaa aattcaatgt 16680 caaagaacca ccagtattcc catcgacacc accaaaaata atatcattta attctgttcc 16740 accattacca cttgccgcta taactaacga atctgttgga cttattggaa attgaaaatc 16800 taatcttcca aaactatttc taatacttaa tgggagtcct gaacggtttg tatctaatac 16860 aagacttcct gtcatagttt gatttatagt tttatctaaa taattttcat gtctatgatt 16920 tactggagct aaattggtat gagtgtgatc aataggtgaa tatcttgctt tcattgtttc 16980 taacaaagtt ttaccagcag atgctgccaa tgctctatca gaagctgaac tatctaatgt 17040 agaaactagt tcatccttac gaacatattg actgaaatca attccacctt cgcttggaga 17100 tgcaccagtc acttcataag gaacaaaggt actaccattc caaccaagca atgtattaac 17160 ttttaaattt tctcccagcg tgacatcttt aagttctcca atagaagagt tggttgataa 17220 tactgatgct cctcctactc gtaatgctcc agtaaaattg ttccaatctc cttcccatat 17280 attgtttcca cccttaagag ctattaaggc tgcctctata ccttgatctt gaacaatttt 17340 tgtaatctct ggtaccaaat cagaaaaatc tgatactgta tgtttgtgac ctttttgaga 17400 aacctcattc catgattcat tattttgtcc tgtagctacc caaatagctc taggatttga 17460 acctgtatca atataagttt gtccataata ttcagcacta atagttggtc tacctgtgcc 17520 gtataacata tttcctctag taatatgaat tgacatttat ttgtcctcct ttataatctt 17580 tctcttatgt atattaaaaa gcttttaaac ctaatataaa aaaagaaccc taaaagatta 17640 aagggttcaa aaactttctg acttttttag aaaaataata ccattggaaa taaatttctg 17700 tccccaataa gtatcttcta tttctttata ctcatcttga tttacaggaa ttggcaatgt 17760 acttcttaca atagtttgct gtttcatgat ctcttttttt aaacgttccc tttttgtata 17820 tttggttacc atataaggca atataaatat agcaggaaaa actaaaagca ttgaaagcaa 17880 aattataaga aaagaacgga aagtaaaaat agaaactact ctatcttctt gctcccacct 17940 tttggctgga agtataattg attgctcagt aaaattccag cgttcaatta ataaatctct 18000 tttttcaatt gcattccgag ctttttctag ctctatttga tgccgaacta aaactctact 18060 attgaatagc tccactttta accctcctaa ccaatcagga atcctgtgtt tttctataaa 18120 attctttaac atgcgaaaaa caagctcgtc ctccttcatc tatctagttt cttaaaataa 18180 tttaatttgg tgtgaagaac ctttcacata atttactact aacatagcta atttaccagg 18240 atctacataa atagaattta atctattata aatgaaattt atttcggtta gatttgaatc 18300 ttttggctca tttactaaaa gattataatt ttctgacaaa tggtattcca atccagaaat 18360 gtttttaata aagactgaag taccatcata agtataatca aaatacttat tggcaacttt 18420 accaaaccat aatacatctt tctctgctag taaatacatg tctgggtctt ctaacaattc 18480 catgaaggaa ataatagaat ctctcgccac cttatttcct gcttctcttg gaagaatatt 18540 gccatatata ctataaaaga ctagatataa atcttcttta gtttttatag aacaagcttc 18600 attatctttt gcagtatatt ctattatgct atagtttata aatgaattta gaataggtcc 18660 gataggttta tcagacacaa aaaatctaat atcttccatt gaacgatttt ctaaatattg 18720 attaaaatat tctgtataga ccaaaggccc gctaccaaag atagcaggct gtccagtaaa 18780 atctaacatc ataaaattcc atctccttca gattctacat aggctttaat agctgtatag 18840 aattctgatg gtgcctcaaa accagcataa tttgctacaa ctaaacactg ttgtacataa 18900 tcaataaatt tatcaaaaat ctctggatta tccttgaaat attcatcagt atccttttcc 18960 atagtaaagg caagctttgc ttcgccctct tcttctaaat attggatacc aactttttca 19020 tcaacaatta attcaaagaa aaatttctca tactgtatat tgtccaattt tatcactcca 19080 atttccttca taggttttta actcacaatc tccacatata ggcatatcag aaaaagcgtt 19140 tgttttatta caagataaac attttacaac agatccatat tttaatatct cagattcttt 19200 tggcgctcct atatataaat tctctctttt tgattttata cctaaagtaa aatcattata 19260 tacttgactt aaatttgttt tataagtctt tatattatcg tcatgaactg agataaaatt 19320 ctctccaaga gaagagaaaa aggtgctttg taaagtattt attgtaaatt cttcaattcc 19380 attattttta tatggatatt gtgtagataa cattgctccc ttatagtctt ttgaaaaaac 19440 ccctagacgt ctataaattt ttctatctac atattcaaca tcattaaagt tgggtttttc 19500 tgtcataaca taagttaaaa atgtataatt atctaacgct gcagaatatc ctccagtata 19560 atgttctccc attggtttat aacatgtagt ccatcctctg ccaacaccca tcattatgaa 19620 atcataagga tgaacagata taaccaaata actggatgga agtatatatg agttctttat 19680 ttgagaatac attaatacga tttcttctcc tagttctgga gtttgattaa taaccttaga 19740 taattttttg ccagaagaat gtttattctc tattaaatct gaatcattta atagccctgc 19800 taatttaaag aagccagcgt ctattttcct ggtttcttta tatcgttcct taaactcttt 19860 tttaaaatct ctttctgctt gtgtttttat agcctttatg ttatctattt taaaaactaa 19920 tcttagatta ttatccatca tagcaattat ttcttttttc ttttcagtaa cttgactgaa 19980 aaaattttca taattattat acgaaaagag actaacccct tgagagaggt taatcttttc 20040 gtactgactt ttcatatttt ctgtcaaatt ttcaccatct tactttccgt cattatttat 20100 agctttggta taacgttctt tttgtgatac atcttccaag tatttttctc gacattcatc 20160 gcacaaatgc gacaatactt cattatctgt atcagaccaa ggaactgtcc ctttgtctaa 20220 aaagcaaaat tcacattgtt cagtttcttt ttttttattg cgacttttat agggaacaaa 20280 acttgtttca tcatcttcat aatttagata atccgattca tcataaacat cttcatctcc 20340 ccagtaacaa taagaaccat tttggtaata gttagaatat ccataattat tatatccagt 20400 ataagaatct tctttatact cataaaattt aggattttga tattggtctt ctaaaatttc 20460 ctttactttt ctctgagtct tatacatttc gttaatataa atgcgttcat aacatgtatg 20520 ttcatccata tatcccgctg ataaatttac tcctgcaaaa ccaaattcag gacaaacttg 20580 acaaatatct gtataagatc cattgtgggt tttaaatcca aagctgttaa tatattcttc 20640 aaaatctata ttatctaaat aataatagac agattctcca aagccttttc gatccaattg 20700 aatagcgaaa ttaatctctg tcaaggctgt ttgtacatct tctccaaaat aattttctac 20760 attttttgta aagaagctag atccaatagt tccaatttcc tcatcccaac aaaataatac 20820 atgaggacga taaccttgtg ttaacatatc taaaatgcta aaaactccac aacgatcatc 20880 cccaccaata ccatcgggac accataaaac atttttttct ttatcgtgaa aaatagtttc 20940 ctctgttgga gctactgtat ggactgtatc caaatgagcc aacataagaa caggaatatc 21000 tcctttagca taaataaaac taccatcaac acgataaata tctctagaat catacattga 21060 ttctatttga cctaataaat aaaaatgaag tccttcttgt gtcatttgta aaatgttttt 21120 aaaggtttgt aaacctaatt ctttcttttg cattttataa aattccccct tttttaataa 21180 tcccaatcat cttcgtcgtc ccaatattca tcttcatcat agtaagcatc ttcgtcaaag 21240 aggcctccag cttcaattga tccattaata gtgaaatcac aagttgggct tatgactgaa 21300 tcaacaccta cttcaaaacg aacattgcat aattcagttt catcagtttc atcataaaaa 21360 tccttattga aatgttttct taaaatgttt tcagaattat tttctccttc aacattgtat 21420 ttttgtttat ctaaaacaat cattggaact ttattcatag ctccatgtgt aatatcatta 21480 tagtgaattt cactactttc tctaatagta ttttctgcat caggagaatc aataaatcca 21540 taagcaacat tatcttctcc aaaagtatct acaaataatt tacgtagagc tttagtatta 21600 tttttattaa ctcctggata tgagcgagat aaaaggatag catctaactc tgtattgaac 21660 atacccattc tacgccaaat tttatctggg atactatctt gacttcctaa aatgatttga 21720 caaataaaac ttgaaccgtc taataaataa gaaactggtc cagcaccata acaaccatca 21780 ataatagaat gacaagaaga ccattctctg gttttgttta cagacatcat taaaatgtca 21840 agagggtcaa tagaaacttc taaaacccca cgagccaata atgattcatt aaaagctgat 21900 agataaatct gtatttggtt aactagtttt ttatttttta gaacacgctt aagagattta 21960 gaaatttttg ctccagcttg gaatgattcg cctaaaattt ctttatttgt cgtaaaacgg 22020 tttttttgta actcttctaa agaaaaacta ctaaaataaa gtgctataat tttcccttca 22080 ttttcttcca tagtcattga tttaatttta gataaaaatg aagaataaaa agcatcttta 22140 atttctgaat ttgacaatgc gttttcaacg cttccttcta gtcttagttt gtttccaaat 22200 ttctgaaaaa taggaaattt tgcttgagcc caatcttctg cccatgcttg tctagtatcc 22260 gtaacgaaac caccattgat gctatataaa atattatcaa tttcttccca agattttttc 22320 atcattggtt ccatcaattt taatttttct tcgatagtat atgttttttc cattgcaaaa 22380 tctccctttt ttattttatt attgtaaatt aaaatttact ccttgctctg ttaaaaaata 22440 ttttcgagga gtgtcagcag ttttttctac tagtcctttt ttattcaatg aagttaatac 22500 acctggaacg ccattaggca tcataaatgc taattctgct acacgtttag aaaatactgc 22560 atcttcttct ttatcaatat gagcctcttg taaagctttt agaacttgcg tacctttttc 22620 tgttaattta atttcttcca tttaaaatcc gctcctattt tttatttatt aattcatttt 22680 ctataatata attataacaa attgaactaa ctttttcaaa ttgttgtttc tcataagcta 22740 ttttatcttt ttatttcttt catttaactt ataattaatt atatcattca atatataaaa 22800 attcaaataa aaaatcccaa tcatctagat tgggattaat tttaaatagt ttatacacca 22860 gcaccagctt ggctaactgt aatttcaatt gtagattcat cagaaagagt agctgttcct 22920 ccagttactt gaccgccttc gtttgttctt aatacgatag ctttaataga ttttccatct 22980 gcaccatttg ctccagggac tccttgatct ccttggtcac ccttatctcc cttaggtccg 23040 gcaacaccag gagttccagc ctcaccttgg tctcctttag gtccagcagg tccgataggt 23100 ccagtttcgc ctgggtctcc tttaggccct ttttgttctt gcaagaaatc tactaaagaa 23160 gcaaatgatt cttgtgtcat agttttacca gttgcaaata atgctttaat ttgttctgcg 23220 ctcattttgt ttcctcctat tttttaaata tatttgagaa cttatctctt tttattatga 23280 aaaagtctta ttctgctttt ccaacttttg ccttacctac tatggctttc cctacaatag 23340 gctcttcacc tgaaacctct ccctttttat aagcaatagc tctatcagta ggacaaatca 23400 tataatagaa atcttccatc ttgtttttac gatgattgat aaccatacaa ttttcttcat 23460 attctcgtcc gcaatgagaa catacataaa tatctttttt cattctcttt cctccttttg 23520 gtaatcgcta atattattaa aaagacctaa tttgaaacaa taaaataaag ccctattata 23580 aaatagggct tttatcatat tattctttta cttcgccagt taataagaaa tcaataaatt 23640 ctttattaac agggttgtct ccagtcaaac gaaattcaaa ggaactatct tcaacaggtt 23700 cccatgaaaa atctaaagaa tctaaaataa ttttagtttt taattgaagt tcttcctccc 23760 gattttcaca tttaaaaata taatccttta aaatttcaaa acaatctttt ttagatatac 23820 ctaatccttg atactttttg aaaagttcat tcattttcca agcttcgtcg agaatttttg 23880 gtagttcctg agaaaagatt tctttccatt catcagaatc aatattttct gtatcttcat 23940 gatattttcc atcaactctc caaataaagc aatcatggat acaagcagtt gttccatctg 24000 gcaattctac aacatcttct tgaagaatat aactgtcaag agtttcgcac catgcagcat 24060 cagaatccaa gatatattct tctaaataat ctgagtaagt agcttttgtt gtataaaccc 24120 aagaatcttc tttagatcca atggcatgaa cagcttcttc actccaaata ttttcctctt 24180 cttcagtaga ccaaactgtt tcttctccac agtattcaca aacataaacg atatctccag 24240 aagtatctaa tactcgaacc ccatagtctt catcaaaacg ttcttcacac gatggacaaa 24300 ttgaacgtga accataataa cattcttcgc aaagtcctga gcaatttgag ggatcatctt 24360 tagagccaca ttcaagacaa acaaaagtac tttgtgcatt aattagaata gttttagctt 24420 cttcagcttg aaaaagaacc ggagatcgta aacctcgaat tgcatcattc cagttgaaag 24480 catctacata gccatattca ttacaatctg aaccatttct ccaacgaaag cgggttaaat 24540 tttcttctat ttggttaatt ttcgcattac cttcccaacc caataaagat aaagttgcat 24600 tactaatcat attttgcaac ccattattca aacgaggata tcctttttgg gacaaaagaa 24660 ctggaacggt ttcttcttca tggtcaactt cagaaacaaa catcatttgt cgccatactt 24720 ttggagctaa ataaccaaat tcttctaaat ctttttccat tacagtaaaa ccaacaaaag 24780 cgtctggtcc aaccatataa cctagtgtac cattattata ttcaccttca tcaggatgca 24840 tgcatgaacg ccaactatta ccataactaa ttaagaataa atcttctggt ctgatagata 24900 gaacaatttt tcgtccatca aaatttgctc ctgatgcaaa gctagccatt tctccaccaa 24960 caattttatt tttaatatca gaaatagctt cttttactcc tttagttgaa gaaatttttt 25020 gagcagttaa tgctgacatg tcaactaaag agtttaaaac tttagttaaa cgttgtccat 25080 gaaaaggtaa cattttaaat tcatctttca taattacctt tttattttca actctaattt 25140 tgtcaaaagt attacaaact gatgttttaa aagtgtcaat gaaattataa gaaatattca 25200 aatcacgtaa atcagaatat aaatcatctt ctacataatt tttcatttct tcaaatgaaa 25260 attcttccat aaaatctttg aaatctttag gatctaaaac aattcttcca tttgttccat 25320 caaagatttt ttgtaggtaa gagtttttca tttccattcc ttttgcataa taatctaaat 25380 attgtgaatc tgataatgaa ccccactcat aaaattcatt aaagatttct tttaaatttg 25440 ctttaacctc ttcaacgtta taatttagca taaatgaaaa actccctttc tttaacttat 25500 ataaatatta tatcatttta aaaattactt ttcaaataat gtttttaaaa tctaaacttt 25560 atttgaaatt tcgtttctaa aacttgatta caaaactatt atataatttt aaaaaaagtt 25620 tttcaaccaa gagcaacgaa gagtttaacc tctagttaaa aacatatttg actttttttg 25680 tttaatatat atatttataa atatttttta tttaaaaagg aagttttttg ttcatgtatt 25740 acaaatctgc tccccactat cactctcttg ggttgacttt ttcatgttaa atatatataa 25800 ttaatataga gttttgagta attctaagag taaaaattct tgactttttg attattatat 25860 gttataatta attataaaga taaataaaga actaaaattt gaaggagtgt atttttaaat 25920 gaaagaagaa attattaaaa aatttatgag tgccttaaat atttcaagag aagcagcttt 25980 agaactatat gattatgacg aacgagtaga aaaaagtaat aaacctgtag aaggagaaat 26040 tgtggttaaa aagattccta aaacttcaaa gaaaaaaggt attcaaaaag aagaattaga 26100 tgcaatgaaa gatttgttgt ctaaaatgtt ccctaatgga gaagagttta aaaatagtca 26160 attatctcca ttatgcgagg aattaaactt gactaataga caaactcctt caagattaaa 26220 agcattaaaa gaagaaggtt tcttagaaga tttaggtggg tcgcctaaaa aatataagat 26280 taaatgagat agaatgtctt ggttttttca gagaaacttt cttatattga gttttaaata 26340 aactaccgat ataggagagg atgaaagttt tatgttaaaa gtaatgaaaa aagatggtca 26400 tattgaagtt tttgatacga agaaaatcca ggaagcaatt ttagcttctg caaggaaaat 26460 tctttctaat gaggaagagt atgatatgca gcaaaaagct ttaattattg cgaatgaggt 26520 agctcgtttc ttaaattcaa ctgaagatga aaatgcagtt gttatgacat ctaaaattca 26580 taatgttgtt cttgagattc tgtctaagga atgggaagtt gttggagctt cttatggaat 26640 gtatagaaac tatagaaaac aaatggctaa aacattttta aaaagttatg aggaatctga 26700 aactgttctt catgatggag ataaagaaaa tgctaataaa gacagtgact tgaatagtac 26760 aaaacaagcc ttaattgcaa acaataatat gcgtggttat atggaaacat ttgaaatgga 26820 taaagattgg attgaagctc ataaacaagg ttggatacac attcatgact tggcagagcg 26880 ttatttacgc cagcaaaatt gttgtttgtt taacatggca ggtttattag atggtgggtt 26940 ccaattaaat ggaacagttt atgcagagcc taaacatttt gattctgcta ttaatgtagt 27000 aggagatgtt actttatttg cttctgcaca gcaatatggt ggttttacta ttcctgaaat 27060 agatactgtt ttagctaaat atgctgaaaa aagttatcaa tcaaatttaa aatttatttt 27120 agataaattt gggaatatta ctaatgaaga tattatggga acactagaac aaacagccta 27180 tgagatgact gttagagaat tagaacaagg atttcaaggc tttgaaacaa aattaaatac 27240 aattagtaat tctttaggac aaatcccttt tgtaacaatt acttttggtt tggatacaac 27300 tcaatgggga agagaaattt ctaaagctat tttgaatgta cgaattaaag gaattggaga 27360 aaataaatca acagctgtat ttcctaaact atctttctta catagaaagg aaattaatgg 27420 cagtccagaa tctcctaact acgacattaa acaattagga attcaatgtt ctcgattaag 27480 actatatcct gattggttat cactagatag tggaaatctt gcagaggtat atgaaagatc 27540 taatcaagtt gtctctggga tgggttgtag ggccttcctt agcccgttct gggatgaaca 27600 agggaatgaa atttacactg gacgatctaa tattggagct gttacgttga atcttcctaa 27660 aattggctta gagtcaaaag gagactggaa taaatttttt gatctaatag ataaatattc 27720 tgatattgtt tttgctattc acgaagatta ttatactaaa attagtaaaa caaaaggctc 27780 ctctaaccct ctatatttct gtgaaggtgg agcttggaca aaagtgggct atgatgaaga 27840 agttggaaaa atatatgaag cctcaactgc ttcattagga tatattggaa tttatgaaac 27900 gttaaaagca atgaatgttc caaaagaaga atatcttaaa catggaacta aaattgttgc 27960 atatttgaaa aaattaacag aaaaagctac aattaattat aatcatttat atgcacttta 28020 ttcaacgcct aaattatgtg ggcgctaatc tagcaataga ttatgaaaaa actgctaaac 28080 tcttaaaaga ggtgtgggag gaatcctgct aacgggggag gctttaaaat gctaatcccg 28140 tgccaagacg aatttttaca aaaacttgca agaaaggaga gttatttgaa attacctaaa 28200 acagccgtaa aaataaaagg ttccaaagac cattgggttg atataaatgg agacatctat 28260 gcattaaatg gtcgaaacaa ccaaaaaaaa tatctgttca aaaagactca attcactgtg 28320 cacggctata aatattgtgg aataaattat attggcaaag gggtagtgaa taaaagagtt 28380 catagactgg tcgctgaagc ttttattcca aaccctaata actacaatat tgtcggacat 28440 aaaaacaata ttaaatctga taacagggtt gagaacttgt attggacgac tacatcagaa 28500 aataccaaaa aagcatttga tgatggtttg gctaaaaatg ccaagggggc agaagatagt 28560 caaagtattc cagttttaat gttctgtacc aaaaccaata agttgattaa ttcttttggt 28620 agtatttctg aggcttcaag agaaacagga atagataaaa ctacaatttc tagacaggct 28680 cgttataaaa gaccttgtag aaaagagttt tatttccgtt ttcctgatga tgcagatgcg 28740 ctagaaaacc agtccttgat agggatgttt gatttcgact cagatagact tattcgaacc 28800 tttattaata gtggagatgc gcataaagaa acaggtatac caaaaagaac aatttgcgac 28860 caagttaaaa aaaatagaaa accaattaga aaatatttgc aagtatattt tctaaaaata 28920 gaaaattcgt aaggtgtaga gactatcgaa atcctagtag gagagtagag tagcgaaagc 28980 gaaagagcag tcccctttga gaaaaagggt gaaaatatag tccaatcccc taataaatat 29040 cgggaaaccg agggtgtaaa tggcagaaag tttatgctat agattccaga aaattaatcg 29100 taaagattat ggagtaattg aagatgtaac tgatagagag tacattacaa attctttcca 29160 tgttccagta tgggaagatg ttagcgttcc tgaaaaaatt gcttttgagg caccattcca 29220 taaacttgca acaggtgggc gaatttctta taatgaattt gtttatggag tagataattc 29280 tgtactagaa caagcaatta attttgctat ggaaaaagga atgtattatg gagttaacgt 29340 tgttgcagga acttgtaacc aatgtggtta tagtggagat ttccatgata actgtcctaa 29400 atgtggtact catgatatta cagtagtaac gagagtttgt gggtatcttt cctttgatca 29460 gattcatggt gactcacgct ataatcctgg taaacaaaaa gaagttaaag aaagagttaa 29520 acataatttc aaataaaagg agaaatatat gaaagaaaag gaacaagagg ttattgaaaa 29580 gttatatgct tatgtatcag aaatggatgt tgttttagca gaaaagaaag tacaaacaat 29640 ttctttaata ctttttattc ttatcgcagg atactgttta atctttgctt gccctgcatt 29700 tattaatgtt gttgctttaa ctggcttatc ttattttgca tttagtttat ttgcaaattt 29760 aatgcttgaa aaacttttgg ataaagaaat ttcaaaaaat gaagttttat tagaacaaat 29820 tcttaaaaat aaagagaaat aaaaaggaag gaagtttttt aaaatgatgt ttattgcagt 29880 tttttgtggg atttgtatcc tattattaat tggttctgga atgaaagcaa atataggaag 29940 agatactacg agattagaga atatggctta cttaatgttt gccatattct taattttgtt 30000 tatcctaatt ttaattgggg gagcataata tggcttattt acacaatata aaaaaatatg 30060 atatggaaaa cagtggctta aaaggagcct cagtttcttt atggtttaat cattgttcac 30120 accattgtcc aggatgctgg aattcagaaa cttgggaaag aaatgaaaac ttatatgagg 30180 ataaccaaga ggtgatagaa gaggtccttg atggtcttga tggcggccca atgaaattaa 30240 atactctcgc tcttttggga ggagaccctc ttagtcctaa aaatataaaa gatacattag 30300 agattttatc ggcagttaaa acagaaagac cagagacaga agttatttgt tggactggtt 30360 ttacttggga acaagtaact aaaagtaaac tacttaaacc agtattagaa tacttagacg 30420 tattaattga tggtcgtttt atgatagatc gtagaatcga aggtaaaaaa tatggctctg 30480 acaatcaaag atgtattgat gtgaaaaaat ctcttcaatg tgataaaata tatttaatgg 30540 agggatttta atgtttaatt ttaataaaaa aacagaaaaa agttctgtaa aaaaatatcg 30600 taaaaaacct gttgaggtag aggtatggct ttttaacaga gaaaatttag aaacagtaga 30660 aagttgggta cgtaagtatt cagataaaat gactttattt tctcaatatg gtgggggaaa 30720 aatttggata gaaataaaaa ctttagaagg tgtaatgaaa gcttctgaag gagattatat 30780 tataaaagga atcaatggag aattatatcc ttgcaaacca gatattttta ttaaaactta 30840 tgaagaggtg acaaattaat gtatgaaaca atagttatag gtggcggacc aggagcaatg 30900 tctgctgtcc tttatttatc tcggcttggt ttaaaaactg catggatcta tggttatggt 30960 tttgggggac aaattcaaaa tacagaagtt gtagataatt atcttggaaa ggggaaaatt 31020 tatgccccaa tcttggtaaa agatatgtac tctcatgtag aagacttaga acatattaca 31080 caaatttttg gtatggttga aaatgttaat aaaattgatg attcaacttt tatagttact 31140 accgatatgg gagaaaaaat tagtggtaat actgtattag ttgcaactgg agcttctcct 31200 agaaaacttg gagttaaagg agaagaagat ttcgctggtt ttggcgtatc ttattgtgct 31260 atttgtgatg ctcctttgtt taaagataaa gaagtagcaa ttgttggtgg tggacaaact 31320 gctttagaag atgcacaaat cctatctcgt catgcaaaaa atgtatattt aattcataga 31380 cgacaagaat ttagagcaac aaatgccgaa gtaaaacaag caaaaaatac tccaaatatc 31440 cattttctct tggatacaca agttacttcc attatgggaa aagacgacgt tgattttatt 31500 gttattaatg aaaagggaat tggtcacaga actttatctg tggatggtat ttttatagct 31560 ataggacaag ttcctcaaac tgatttttta actgaaaact ttgattatat tttacgagat 31620 ggttatgtag aaacgatgag aaataataag gtagaagatt taggcatctt tgccattggg 31680 gatgtagttt atgggaataa taaacaaatt gctattgctg taggagaagg tgcgaatgcc 31740 gcaatagaaa ttaataaata tttatcagac cctaattttt gattttttat gtataaaatg 31800 ttaatataaa tatataacaa aataggagga caaaattatg actgtatatg ctatgtcaga 31860 tatccatgga aattttcaag ccttagaaaa agctttagat ttcttattta ataaaacaaa 31920 aatttctatt ggtgaggata aacttatttt tcttggagat tatattgatc gttctcctga 31980 tggatattct gttttaaaga ctatttatga cttacaacaa aaacatggaa aagaaaatat 32040 tattgtttta atggggaatc atgatgaaat gtttttggag tggttaaaaa atccttctaa 32100 tactcttcat ttaattaatg ataagccatt aactactgtc aaaagtttta taaaacctct 32160 taacaaggag tggaacttaa tttttgacgg tgtagatgct atttatgaca taagtgatcc 32220 taataatcaa attaaagtta cacaagaagt tgttgataca attaaaacag aatacaaaga 32280 attattagat tggtatcaaa atttagatta tttttatgaa catgggaatg ctttgtttgt 32340 tcatgccggt tttgaagaag agtcaagtgg gcattggaca gattcctcta aagaagaaat 32400 gacttggaaa tacccagctt ctcttggtta tacgccgtgg aataaagaaa ttgtagcagg 32460 tcatataatg acgagagagt tgcatccaga aggagttccc caagagaaag aagattcaat 32520 ttatagaaat aaggatcata tttttattga cggggctgcg ccgatcacaa atcatattaa 32580 tattttggct tatgatgaaa atggttatct atactatgat gctgagacag gagaaatttt 32640 accagaataa cctaagcttt ttattgtcat tattaatttt ccctttttat tatctttagc 32700 agagataaat aaagagagga gaattaatat gagaatcaat gcagataaaa taagagaata 32760 tagagaagag catggaatta gtatcgatga gatggctgaa attcttaata tagatggaga 32820 agcactttat gaatacgaaa gtggagttcc atggtttgaa gaagatgcct taactatttt 32880 tacgattaat aatatttgtg ataatttaga ttttattgct atagatgttg aggaattatt 32940 tgaagaatat aatatttaat tttataaaat tcaactattg gatttccttt agttgaattt 33000 ttttatttaa aattatataa tatatatgta agttaattaa gaaaggaagt tgcttaatga 33060 aagaagaaat tttgaacatc ctattagttg atttgaaaga agatttagat agagaattaa 33120 ttgtatcagt agacttactt taggaggcga aaagtataaa aaagattatt aagattcatg 33180 tagatggaag ctataaacaa aaagttaatc ctaacgtagc tggttggggc ttttgtgttg 33240 aagcagagga gcaagaagtt ttatatgctg cttccggtat tgtaaacaat ccagtttcta 33300 gacagattga tggagaaatt caagccgcta aagaagctat taattggtgc cttgaaaatg 33360 ttatagataa agtagataca aaaattgaaa tatatcacga ttatgaaggc ttaggaaaat 33420 gggctgacaa tctttggaag acaaataaac ccttaactac agaatataag ctttttgttt 33480 ctaacgctcg attctcagga cttaaagttt attttatatg ggaaaaaggt cataataata 33540 atttttttaa tgaaattgca gatgagttag caacttctgc cgttgaagat tctgttagtt 33600 aaaatatcca acagaaaata atattctata agtagaataa ataaaaatag gaggttttaa 33660 aatgaataaa gatttattag taaggggtgt aaaaagtaca gtaaaaacag tagaaacaaa 33720 attaaataga aaagttcatt ctgtatattt gacaggtagc gctctttatg gagtttctac 33780 tcctgaaagt gattttgatt ttaaggtcta tgtatacccc accttttcag atttgtgctt 33840 taacgaaaag ctttctaaag aggtggctat taatgaaaac aatgtagaac atgtttctgt 33900 aagagatgtt cgattccaat atgatgagct aaataaattg tccttcaata gcgcccattt 33960 acttcatcct cctcttttgg gaaaaagatt atttacagaa gaacaattaa cacaagtttt 34020 agaagagaga aaagataatt ttctttttag tcttgctggg gtctctttat cagagtctaa 34080 aaatgaagat tcagggaaaa aactttcaag aatgttattg ttagaaaata tgtttactgc 34140 tgttttagag gataaattta ctctaaatta tttctttgat aaagattcct ctaaagaagt 34200 attggttaga gaaaatcttc atagatatac agactttgct aaacttactc ctgaagaatt 34260 gatgttgaca gccaaagact ttaaaaaaca atataaggtt gactataaaa aattagagaa 34320 tgttttaaaa aatcaattat tagaaacagt aaaaacattt tacaatgtag gaaaggaagt 34380 atattaatgt ttaacgtatt aattacaaaa gatatggtaa tgaatgggat tacaatcaaa 34440 aatgttcctg ttaaacaatt agaaggtaag gatgctcctg ggattacaaa aggggttgct 34500 agtaaaattc aagatttagt ttggaaagct aacgcactta aagttgatac aatagatttt 34560 agttataatt tagggaaaga ataggagaag agatttttat gtttcttgat tatgatgaag 34620 gtttagtaga agattatgta gacaaaaata ttgttcagcc tgtattaaaa ttaattgaac 34680 agacaataaa agatgctggt aaagatcctt cagaatggga ttttttaaat ctatctattt 34740 ctttagatga tactgaatta aaatatatta atctttatgg ggaaaacaaa ttttattttc 34800 gatttatgga agaaaatatc catgtatata ttcacaacaa agaattgtat atcacaaaaa 34860 agagaaaatc tggaattcag ctttctgact acttaaacga agaaaatatg gagctaacag 34920 caaatcaaat tagtagtttt ttagaacaat actttaagga ggaagaataa ttgaatacat 34980 taaaaatgga tcactttgtt gcatctagta tttctgaaat cctagataat ggagttaatt 35040 ctttaggagc tcgtcctaaa tacgcctctg atgggaaaac cgccaatagt aaatatataa 35100 cagatgtatt tatcaaattt tatttagaca agggagagtt gcccattagt agattgcgtc 35160 ctgtatatac tggattagct agacaggaac ttttttggat atatcaagat caaacatctg 35220 atttgcagcc attaagagat agaggagtcc attggtggga agattgggct ttagaggatg 35280 gaactattgg acaacgttat ggagcaacag ttaatcgcta taatctaatt gatagattaa 35340 tagaaggact agaaaagaac ccatataatc gtagaaatat tattaattta tggcaatata 35400 aagacttaga tgaaacagaa ggtttaatgc catgtgcttt tcaaactatt tgggatgtta 35460 gagaagttaa tggagaaatg tttttggata tgagtttaat tcaacgcagt tcagattttc 35520 tcatagccgg catgggtatc aatcaaatcc aatatgtagc attacaaatg atggtggcta 35580 aacattttgg gtggaaggta ggaacttttt cttggcatgt aatgaacttg catatttatg 35640 atagatattt tgctcaagct caagaattag ttgctagatt tgaaaatatg aaccaagagg 35700 aacaaaaaga aattgagttt tacttggacg ttcctgataa aactaatttt tatgatataa 35760 aaccaagcga ttttgtacta aagggctatt cctctgtggg agatcagatg aaatttgata 35820 tggcaattta ataataaatt aagacacctt aaatggtgtc ttttttttgt actttttagc 35880 tataggattt taacgtattt gagctaggaa attctcaaaa aataaaagga aggtgtaaaa 35940 tatgtttaaa gaaaaagatt tacttattcc aattggcgta tttacagtcg gggagtctaa 36000 agaagattat atcgaaatgg acccatatga aaaaggcact gttgacaagt taaaagaata 36060 taatgaaaat ggcgtagtag aaatgaccgc agaaggtgaa aagaaagatt ttgatggata 36120 ttcaaaagga gatttcttct cttttggaga taagggattt ccagtagttc gaagcaatga 36180 aatttttact aaactagatg ttaatggaca attgctatca ttccccaatc ataaacttat 36240 ggaaggtgat aaataatgac tcgtttttat aaaggaatcg ctggtcgtcg aggagctaat 36300 ccatcaggag tagttattca caatgatgca ggctctgttt atgctaatgc tgcattctat 36360 cgtggatggt taccttctca tgatgcagaa aatggttttg ctcattatta tgtagctagc 36420 gatggaacat tccaagcaga agatgaaatg aatatggcat ggcatactgc aaattcaact 36480 ggtaatgcat attatatcgg tattgaagca tgtcaatcaa tgggaccaga aaatattttt 36540 cgacaaaatg aagaaaattc tattaaatta gcagcccaaa tcttaaagag atatggttta 36600 caacctaata gaaatactgt gaaattgcat aaacaatttt caccaacttc ttgtcctcac 36660 cgctctgttg cactacatgg agatggatat gtaatgcaag attattttat tgctcgtatt 36720 aaatcttata tgggttctaa accagctcca gcgccagctc cagcaccaag tactggaact 36780 agaaaatata atattgacct agttaatatg aattcaaaag ctttccaaat caaaggttgg 36840 tttaccccag ctaaggctac aaaaggtcaa gatatttggg tttatattat gaaaaaaggt 36900 ggaccagaaa ttgcccgctt taaagcgaaa aaaattcaac gtccagacgt tcaaaaagca 36960 gtttcaaatc ctaacggagc agatgttggt ttccaagtag atgctcttac gccagaaggt 37020 gttctaggaa aagattttga tttattactt cgttacaaca acgataacaa agaagaaatt 37080 cgcactaaag agcattggaa agctcctgca ttaattaatg aaggatacct agataaagtt 37140 gctggagatg ctaaaggtgt tacattcgcc ggatggcatt taaatacacg tcaaaaagat 37200 ggatttaaac attatctatt tgtaactgat gcaaaaactg gaaaagaata tgtacgattc 37260 gatattacag gagggtctta cttagcttct ccagatatca ctaagaaata tggtccagaa 37320 atcgctcaac gtggtaactg tcgcttccat aattggtgcg cattagcagc aaatcatcca 37380 gccagaggta aaactgttaa cattattagc cgttatgctc ctgcaaaaga aattgataca 37440 aaaacttcta cagacaaact gtggggaact tatagactat aaaaataaga ttaaaaatac 37500 ccttagaaat aagggtattt ttttgctttt tatacataaa tttgttataa taataacaaa 37560 tagaataaaa aaaaggaagt gtttttattg atttatgctg tagcagcaat tggaccaaat 37620 ggagaattag ggttaaataa taaattaatt taccattttt cagaagatat gaaaagattt 37680 aaaactttaa cttggaatag aaatatttat atggggtata aaacttggga atcactgcca 37740 gtagaagcat tacccaatag gatgaataca gtagtgctgg atatggaaag aggaatatca 37800 aaaaaactcc agacagccat gaaaaacaat gacaatttaa aagttataga tgagtccatg 37860 tttcttgctc tcttggagat tcattcatta tcctcagatg ttattgtagt tattggtgga 37920 ggaaggttat atgcacaaac tcaatattat tggagtagat tgtatttaac aagattttat 37980 gaaaagagac aagcagatac gtattttcct gaaatagatt ataataatta taccatgaag 38040 agtttccaaa agcaagaaga ttgtgaattt attgattttt atcgtataaa atgatataat 38100 taatatatac ttaaaaagga gataataaaa tggaaaaaat tgtagaatat attttagtta 38160 ataaggaatt aaatatgagc gttggtaaaa tcgctggtca agttgctcat gtacaaacag 38220 ttattgataa taaaattttt gaattagttc tatcagaaga gattcttgac gaagatagta 38280 tggatagttt tgctaaagaa aaagaattaa ccaataatta ttatgattgg ctttattctg 38340 gatctcaaac aaaaattatc ttacgagcca aagaaaaaga tttgcttaag gcaattgata 38400 tgggagcagt atatattaga gataatggct taactgagat tccttcaggt tcactaaccg 38460 ttgtaggttt ctttccacaa ccgaaagaca atctagtaga tttcaccaaa aaatttcaat 38520 tgttataaga ggagattaat ataatgaaaa ttattggata tattttaatg gaattaggca 38580 gaagtcgccc atcctctata ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg 38640 gtaactcgaa gaccgaaaag ataaatgcta taattagttt gattccaagc atgatggtgc 38700 aattccagtg tgaaggcgct caagtgaaag atattgagtt agcgccacaa gtaaaactgt 38760 aagacttgaa gacattcgtg acgatgcgag tgtaacaatg attatgatta gagtgaaaaa 38820 caaactgaat ctgtcataac gttgtgtggt tagtatttcg accgacgata aaaataaatc 38880 aaaaaaaccg taggaactac ggggatagct tggtaaataa gagaaaccgc tgttagtgaa 38940 gaaataagct atcaagtatg ctctattccc aagaagctcc cacttcaagt gttaagaacc 39000 gttaggttta gctaagtgga gagtagttca ctggaaagaa cttcctataa ccagactttt 39060 gtaaaactcg aatttaattt aatttgattt tttcagaata agatgatata atattattat 39120 aataaatgat aaaaaggaga aaaacattat gaagaaaaca aaattttatg gagtcgtctc 39180 tacaagtaaa aaagctcgtg aggtagtgcg aacaaaagat tcaaaaggac gttggacttc 39240 tgaggttcaa gaaatttctc gtccaattga tatcttagta acttctgatt ttactaatcg 39300 tattgatgct ttaaatgata ttgaagcaca agcagctaaa ttaggtacac taaaatttac 39360 tagtgctttt aaataacata taatattgaa agggaatttt tataattccc tttttacata 39420 gaaatataaa aataaaggag agtggagttc catgaaaatt aaaccttctc ataaaatgaa 39480 attggatgaa gtaggagatt acctacaaaa ggtttctcgt ggagtaggaa ctattaagcc 39540 aaaaaagggt aaaggctcgt ataaaagaaa gcctaaacat aaagggaagt gggattaatg 39600 gataaagata taaaatatac gatctcaagt ggaggcgaaa caattggcta ttggacgcaa 39660 gaagaatatg aaatctggat ggcgaagatt catggtactc acgaatactc tgaaggagaa 39720 caaagaaaaa ttgaagaagt tagaaaaaga ttaaataata tatctatgat aaaaaatagt 39780 gatggtagtt atagatttat taaagaggag gaaaacagtg tcaattaatt tattatatgg 39840 taaaccggta gcagattcaa tttataaaga tattcattat ccaaataaaa aagttttgca 39900 tattttccag gttggagatc tagaggaatc taaccgctat atcaaaaata agattaaaaa 39960 ggctgaatct ttaggtattc caactcagct acacaaggct gaaactaaag aaaaggtcga 40020 taaaatttta aaagaacttg tatctttaac aaagattcct aatcgagata aagtatctgg 40080 tatatttgtt caacttcctt ttgaatttga tcgccttatg gaagatacat ttttgcgtgt 40140 aattccccca gaatttgatg ttgatggttt aacacattat aatttaggac gtctactaga 40200 tggaagcaga actgacctaa attttgtccc agcaacagcg catggggtag tagatatgct 40260 aaaatattat aacatcccaa tagagggaaa aactgtaggt attattggtc gttccaattt 40320 agtcggaaag ccacttattc ctcttctttt ggaagaaaat gcaactgtca ttagtatgaa 40380 ttcctatact gataaaaaga atttaatttc tctcagcaaa caatgtgata tattggtggt 40440 agcaattgga aaagctaact ttatagacag caatttcatt aaagataatg ctactgttat 40500 agatgttggt attaattttg atgaatctgg gaagttagtt ggagatgtag actctaattc 40560 tttgaaaaaa gaaggttgga atggcaatct ttctccagta ccaggtggag ttggaccttt 40620 gacaatcgct aatttaatga gaaatgtaaa tataggagcg tggagataat gatggattat 40680 tttagtgtag agcaagttaa acatatttta ttattaaaag atttacaggg gtatactatt 40740 gatgctattt ttcaagatga tactccagag cagagatata gtaacttaat caaagtattt 40800 ttgggattag aaaatgtttt tatttgccaa aaaaaagatt atcttactaa gggagattta 40860 tattccgata attctatggt agaatctatt gccaaaaaaa ttaaagaatg cctgtaaaag 40920 ggcatttttt aattgaattt attaatttaa aatgatataa ttaattataa agttaaataa 40980 ggaggtaaaa atgatagagt tagcctctca tattgtagat ttaagtttag aacataaaaa 41040 atatatttgt catttacagt tacaaaaaat aacgtactat actgtattgc aggggttaaa 41100 attaaattta atagataaac acactctcga agaattatat aaatataagt ctgatagatt 41160 tacaccatca agatatggtc ctcgatccga agctatattt cttcgatact gtgcatttgg 41220 ggcggaaaga atttttacta attcctttag cagttcatat ttaagaaata tggatttctc 41280 ccacttgaac cctattattc ttcctctttt ggaagaacag acaatggatt tatgtaggaa 41340 aagtatgaaa aatcctattt ttgaagaacg gtttaaagat aataaagaat ttagtattga 41400 agatatattt gaacttagtg aaaaatattc agatttgaaa taaaaaatat aaaatgatat 41460 aattaattat aaagttaaat aaggaggaaa ttttaaatga aaaaaagttt agtaggattc 41520 tcaattgttg caatgtttgg tttaggttta gtaggatgta ctaaaggaga agttcctgat 41580 ggacaatgga aaagcgaaga ttctactgaa cagacagtag aaagtagcac agtagaatcg 41640 tctgaacaaa aagaacctta taaaaaagat gaggttcaat tatctacctt agaacaacaa 41700 gttttagatg cttttcaaca aagtttccaa tcaacaggac cggttcgttt tgattctgta 41760 aaaaaatatt atattattac accaacagat cctcaattta cgcaagaaat ggcagctatc 41820 atggatggca cattaagttt agacgtatgg actaatttgg ttcaaaatgt tcaacaaaca 41880 tctttaggtt tacaagatac tcttggtagt ggatatacaa ttattattga aaatccattt 41940 gaaacaggtc agatgttttt ggctgtaagt gatggagaag ttgttttcga tggtttgaca 42000 gagtatatga ctggtcaaaa ctcataactt gaaaaaatat gaataacatg ttataatatt 42060 tatataagat aaagttactt gagtggtcgc aaaaaggaaa gacgcatggt ttaatcttcc 42120 atgagacgtt ggttcgagtc caacctcaag aatatgagga ttcgctatcc cttcacctta 42180 tgcatgacga tgcagtgtaa gttcgaatcc tactatctca gttaaacagg gacttgagct 42240 aattcatttt agccagtcct tatcccgaaa gggaaaaata atttgacatt ttaagcataa 42300 tatgttataa ttattatata ataaagataa gaaatatgaa acggcttgtt ggtcaagaag 42360 ttaagacgct cgactttcac tcgggaatca caggtgcaaa tcctgtacaa gtcattaatt 42420 ttattaaaag gaggagtaca atgtcaacta tagttgggaa ttttagttta gattatccaa 42480 gaactattag tggtaaacag aaaattgaag ctttagaaaa gatcaatgaa atgggagata 42540 aaatcattga tcaattcttc tctagtcaag agacaaatgc tccaactttt tttatcaata 42600 accgacttac tagaacatat ggtagatatt tccctagtga aaataaaatc gaaatgtctg 42660 gtcagtttac aaagatcgct ctatatcttg caaaagacga atctttattt gaaaaagttt 42720 tgaaacacga acttgctcac tggtacttac ataaaagtgg gcgtaaatac agcgatggag 42780 agcctgaatt tgaaaaattg ttgtcttata ttgattcact ttcgtcagga gcaactaata 42840 aaaagctaca attagctcca acaacacctc ttttatcatt taaagttcat tctgaatgtg 42900 ataagtgcgg agcttctaat ttttcaaaca gacgattaaa taatcgttat acccatgttg 42960 attgtggtgg cttgctagtt gataaagaat tggttgtagt taaatcttaa aagatatttg 43020 aaaaaaatct ttcaaaatgt tataattaat atgtaaaaag aaataaaaaa acttatttta 43080 aaaggagaga ttcatcatgg aaaaacaaac taaagcagat atgtatcgta gcgcagcaaa 43140 attgttggaa gtaaatgctc ctgaatatgc agaaatgtta ttggaagatg ctcaaagaat 43200 tgaagctcgt aacgcaagtc gtcgaaacgt tgatactaag gttcaaaaag aaaataaaca 43260 catcgctgct ttagtaattg agtttttcca caatgacgct gaagatggaa tcgcttatga 43320 cagtggcgaa attgcaaatg gagttggaat cgaagtttct cctcaaaaaa tgtctgcaat 43380 tatgcgttta gttggagacg aagtagataa agtaggtcaa gctacgaaca ataaagatca 43440 tgttggatat aaaaagaaag gaactgaatt agtttctgat gaaaagtagg acaaaatgtc 43500 ttaattaaat atccccatat tttattgggg atacaccagg gtataggaga atttggctaa 43560 tccgctgcat ttgggatgca gagattgttg gttcaaatcc agctaccctg attgaaacaa 43620 cgtgttagaa cgttgggcgt gaaggaattt attccaacac gtttaaacat tcacctatga 43680 tgaaatggta tcatgattga ctgttaatca attcttgcag gttcgaatcc tgctgggtga 43740 gttaaagaat cttacagcaa atattaatac ttttaataat atttgatgag aaaaataagg 43800 tctaggttcg acccctagta taagttcggt acaacttata tggtgtaatg gtagcacaca 43860 aaaagcagat tcttgttttt ggggaaggta cctccccagt atttggagga ttatgcaaat 43920 tggtgaagca agtggtctgt aaaaccatga cgtaagatac attgggggtt cgaatccctt 43980 gtcctccatt ttggcgctat ggtcaagcgg ttaagacact ggtctttcta accagaatcc 44040 cgagttcgaa tctcggtagc gtcataaaga acgatacagc aaaaccttat tcaaatatta 44100 cagaagagat tatgtaagga gccgtagata ccaaccgaaa aatctacgag ttcaactatg 44160 gttggcgttt gagttttttc taggtgcttt tcttgagttt acgttagaac tcgaaaaagg 44220 tctaaacttc gttcttgata aataaacaca actttccttt cttcctgtcc atgtcactgg 44280 acaggtaaca cgggtatggt gggattggca gacacatcag acttaagatc tgacgagaag 44340 tatctctctc atgcaggttc gactcctgct atccgtataa agaacctaac agcaatcaca 44400 tttaatggga taatagtata tctaataata tataggttct tgttttttat aatggctact 44460 gcccatcgca acctacatac ggttcgatga tagtcttccc tctgctaagg gtttatcttt 44520 acctgtgtgt agtagcttaa aagaccaaat taaacaggta atccggaaag tagctcagct 44580 tggtagagcg caggctttgg gagtctgatg tcgcaggttc aagtcctgtc tttccgattt 44640 gcgtaagtta atcattaaaa ttagccccta gtcaaatttc cactttggcg gctaggttga 44700 ggcgcacaat aggcataaag acacctcgta aaagggcaag cgcagtgtcc ttacccaaat 44760 aaaacagcgg cagcctttac gctggtttcg tataatggct attacagttg acttgtaatc 44820 ttcaaatggg agttcgattc tctcaaccag catttataaa tgaatgaggc attggttagg 44880 tctaaaatag atatagggat atttagttat ctacgggtgc tagatattgt gtaattcatt 44940 ttatttttcg atgattcgtt caaatggata ggacgctaga ctacgaatct agtaatagag 45000 gttcaagtcc tctatcgtcg gtagtacaag gaatgatatt tcgcttatac gaaacgttaa 45060 gagattctca cactgacgtc ccaatagaga aggatttgaa tatcttcctt ctcttggttt 45120 tcaatactat taggatttat tagagcctgc cagtcaggct cttaatattg tcaaaaataa 45180 ggaaaagtta gaggagatgg ttatgataat tacattagcc ggagttattg gtgtaggtaa 45240 atcatcaatg gcagaaatat tagcaaatct tttaaataca aaagcagttt atgaaccggt 45300 agaggaaaat ccaatgcttg aattgttcta ccaagataaa gcaaagtatg gactactttt 45360 ccaaatagat atgttgtcta aacgcttcga aatgattcaa gaagctatga gtgtagataa 45420 cggtatttta gaccgttcta tttttgaaga cagtatcttc ttagaccaac tagtacaaga 45480 gggtcatgtc acagaaatgg aacagggagt ttatcaccga ttgcttaatc gtatgttaaa 45540 agaattagaa ccattaccta aaaagactcc cgatttaatg attgtattaa attgttcttt 45600 tgaagaagaa atcaaacgaa tcaatgcacg tgctcgtgag tttgagaaag tagaagaggg 45660 tactgaatta tacgaatact ttaaacacca tcacgagaac tatcaaaatt ggttacaaaa 45720 agatatggga ttccctaaaa ttgttttaga tgtaacagat aaagactttg tgaatgataa 45780 aggagaccgt gtttacttgt taacaattat tttgagtaag ttgtgtgaag tcggggcact 45840 atctactgta gatacggtta aatgtttatg tgagttaaat ggcttacctt ggtgtaaaga 45900 ccacgctaca caagttgcat tgaagctata caatagatta gagggtaaac tacctttcca 45960 tgaattaagc aagtttactg acaatcaaga cttatacaca ctagagaaag ctgtttaatg 46020 gaggaaaaat aacaaatgga caagttaaaa aagttttgga aggataacaa agaaaatatt 46080 gaaaaattat tcacttacga atattataaa gatggtttaa ctggttggaa caaatcagct 46140 cgtgtattgt ggtttgtcgg aatggcagtc caagtattta ccggtattgt tctacctatg 46200 ctacaaggtc aagggttaac aatgacaggg tttactgcta caattgcagg aattattgga 46260 tttacatgta ctgtagcaat tacaatgggt aaatcaatta atggtatttt aggattcatt 46320 tctgctattt tattaatcgg tgtagcaact gtaacaggta actatagtga tattattatg 46380 caaactgctt atattgtttt gttagacatt cctatcatct tctctaaaca atggaatgag 46440 ggtttgaaac ctcgtaaaat gcaacctgta cacatgctta aaacattagg tcttattgtt 46500 gttttctggg ttgctttata cttcttagat acagttgttt tacactcacc gcaggctttc 46560 ttagatgcaa caagtgcaat gatcggttta gttggagctg tactgtgtgt gaaaggcttt 46620 agagcgcaat attacttctg gacttttcaa ggtctaatgt cggtagctct atggattcaa 46680 acagctttac atggacatgc ggtttatgta ctaatgctaa cttacatgtt atatctggca 46740 aatgatgtat tagcattcac aaactctaaa tggtttaaaa atggtaaaat caaagaaatt 46800 aaataattta ttagacagat aggagagctt gaacaatatg aagaaaagat tgaataaact 46860 ataaatatct tataaaggag gtatttatag tatgagtaga agcagaaaaa aacaaccata 46920 ttcaagatat gtttgtatga agaattcttc tgtcagaaaa tccaagaaac aagtaaatcg 46980 tctttatcga agaagaatga aacaaggtcg atatgatgaa gatgccaaaa ttccaagttt 47040 atataaaaga catgtagatt tggattggga ttatgaaatg ataaaaggat attttcccaa 47100 agaaaagtat cctgattttt atgaaaaact tatgagaaaa taaaactccc taaataaggg 47160 agttttttat ttgaaaaaat taatttaaaa tgatataatt aattataagg ttaaataaag 47220 gagattttaa aaatgaatga aagagaaaga atatttcata aagctaaaaa tatcactgcc 47280 aaaactgaat taattagagg cgtagtacaa aatccaatgc tccccgaaga agtagattta 47340 gaagaattca aaaaagaaat tcagcgtatc aaacgagaat taacagatta tcaaaatgaa 47400 gttctcttat attttgcctc tattggggat gaagagaaag aaagaattaa gcaagaacag 47460 ttagaattat ctgaatactg ggaacaaaag agggaaacta tactatgaaa gtatttgtag 47520 atgatttaag gaagtgtcct gaaagatatg aattatttag gagcgcagaa gattttcttg 47580 attggagaaa gaaaaataat gatgtagaaa ttgatatttt ggcattggat catgacctag 47640 gagaaaactg tttgaatggg tacgaattag ttcaagaatt agtaaaatct actagaatgg 47700 acaatattaa aactattatc tttcacacag ataatttaat tggtatgaaa aatatgtatt 47760 attatttaag taatgcccaa aagcatggga ttattagtaa aaatattgta ataaacccaa 47820 ataaatataa ttgtttagat ggtaagttta ctcatagtct atatcgtatt attaaataat 47880 ttgaaattat taatataata tattataatt atattataag ataattaatc atatttaata 47940 ttattaatga gattaaaaag ttgttgaaga agaaaatttt gaagatgaag aaagttggtt 48000 agataaatga aagtagggga cactgttctt attcttaaag actttgaaga ttttgcttgt 48060 aaaggatgta ttggagtaat tgaagaaata aatatagtct tcaataaagt acgttttgtt 48120 tcaaaagaag gcactgaaat gatttgtcct ttttttgacg aagaactgga ggtaatctaa 48180 tggtatttgt atttttattg ttaaatggag gagttatatt aggaacgatt ggtttaattt 48240 ttcttattat tgctacaatt ttatccatta agccaagcac tagagaaaaa tatattgata 48300 taattattaa attttattat gttgcaggta tactttatat tgtttcagcc tttttctctt 48360 ttgaagtttt tgctatattc ttaaaagttt tggttggatt aatatgctta tataatgttt 48420 atttaatcaa aaaaaataaa aattgacttt tttagcataa catgttataa tatttatata 48480 agttaggaag aaaaaacttc ctaatatgtc gctatagtgt aatggatagc acaagaaact 48540 tctaatttct aagtttaggt tcgaatccta atagcgatgt tcacagtaga atagcttatg 48600 tttggttaaa gcactcacct tataagtgag agagcgtggg ttcgaacccc acttctacta 48660 taaactatac aaagtaagga agctctttgt aattaggaca aaatgtctta aaagacatgt 48720 aaattatttt aaagacttat acaggtgcga aattaattca acaggtagaa ttatttagat 48780 gggttcaaat cccatatttc gcttaggtaa agaatcttac agcaaatatc atatgtttaa 48840 taggctaatt caaatatatt atataagatt cttgaagtta tttttagccg attaagagat 48900 aaaggagata aataactatg acatttttag ataatttgaa agaggaagca acagttggac 48960 gcacttttaa tggtgcaact acctatacta cttcactaaa tgctaatgtt gacttttttg 49020 cattaggtgg atctatgcga taccaaagtg ttggagataa ggtttcatta tttgaaaaag 49080 catataatga agatagagct gttgcattga gaaatctagt ttatatgcga gatattcgta 49140 atggtggact aggtgaaaga gatatgttcc acgcaggaat ttggttctta gctaaaaaga 49200 aagatgaaaa agcacttcat gttatcatga ataatgtagc tgattttggt cgttgggatg 49260 atctattttt cattatgaaa caagatgaaa aattgtttga ttatggacta attatcatta 49320 aaactcagct tctgaaagat atggataata tgcgaaaggg caaagaaatt tctttattag 49380 gaaaatggtt gcctaatatc aatgctaaaa acgaagaacg aagagctttt ggtcttagag 49440 ttgctaatgc tttaggatat actaaaccta gtgattataa ggtttaccga aagatgttgg 49500 ttaatctaag agaaaaatta gatttagtag aagtaaaatt ggctgctaaa gattatgatt 49560 caattgattt ttctaagcta ccttctcgag caggatttaa gtatcgctca gcattttggc 49620 gccatatttc agatcgttat tctgaatttt tggatgctgt aaataatggt gaagctacaa 49680 tgaatggaaa tttggtaatg ccagatgaaa ttattaaagc ttatggatat cgtagttgta 49740 tttcttatga agaacaacct gaattagaag ctgcatggaa atctctaaaa gatactgtta 49800 agggttctga cgagaatgta attgtagttg cagatacttc tgcttctatg tggggtaaac 49860 catgggaagt agctgaaggc ttggcaattt atactgctga acgcttggaa ggaccattca 49920 agaataattt tatcactttc tctgaatgcc cacaattagt aactttacca aatgattgct 49980 ccttacgaga taaaatgaat gaatattctc gtcattctat tatcgaaaac acaaatattg 50040 aagcagtatt taatcttatc ttagatactg ctatcaagaa taacactcca aaagaagaag 50100 ttccatctaa gattatcatt atttcagata tggaatttga ttttcaacga ggaaatggcg 50160 ttacacattt caaacttgca aaatctcgtt ttgaggacgc tggttatgaa atgccagatg 50220 tagtcttttg gaatgtagaa gctagattag gaaacttgcc tgttcgttat aatgaacaag 50280 gagccgcatt agtatctgga ttcagcacaa atattctaac ttccatctta aatgggaaac 50340 tagaaactcc agaagctatg atgttagaaa cgttatctaa tccagcattt gacttcattg 50400 atgaagccat ctaaaatgcc cccttttcta cctcttaatt gaggtagtta cgcttcttta 50460 gctcagtagg tagtagcgcc agtctgaaga actggaggtc cctggttcga ttccaggagg 50520 aagcattaat ccagcccagg aaagtgccaa cactatattc gctctcttgg gctttttcat 50580 atttgaaaaa atatattaat catgttataa ttaattataa agttaattaa gaactttaaa 50640 aatgtccctg tcgccaagag gcttaaggct atggattgca aacccataat acgtgagttc 50700 gaatctcacc agggacttat ttcttatgaa agaaggggaa aaacttgact aaatttatta 50760 cgagtgattg gcatttcttc cataataatg cttgtggtcc agaaggattc atttctacta 50820 gaaaaaagtt ttcctctgta gaagagatga acgataatat tattaaaact attaatcaaa 50880 aggttggtag agatgatact attattcatt taggagatat tggttttggt aaacctaaaa 50940 aattgtttga aacattagaa aagatcaatg gacaaattat tttaattaag ggaaatcatg 51000 atagttcaaa attatttaag tatattgtaa ataataatta tcattatcca tatagtaatc 51060 ctaaaaaacg taaacaatct cctgacagag ttaaattttt agttgaagaa gttggtttaa 51120 gggaaaaaat gaatggtaaa gtttactatt taactcacta tccatttcaa ttaggagagc 51180 aacgtaaaaa tatgcgttca atttgtgggc atattcatga gcaaattgcc cctgacgcta 51240 atatgattaa tgtaggcatt gattctcctg aaattacttc aaagatttct aactttggag 51300 aacctcttct tctggaagat gcaatagaat tagttgataa aaaatgggaa aaatggtata 51360 atactataag atttttggga agttaatctt cccttttacg gagctatgac tgagaggctg 51420 aaggtgacgc actgctaatg cgttagtcga cataaagttg gctcatgggt tcgaatccca 51480 ttagctcctt ttattaaaaa tagggggttt ttatatgttc ggtgctaaat tagcattttt 51540 tctatatgtt gtcgcaataa ttacatttat ggttttactt gctccaattg tattatatct 51600 aatttggaat tttgtagtag caatagcatt tccaggacta tttacattaa cttttttcca 51660 atgttttagt ataacattat tgcttacttt tataaaagca attttcatta ataaaaaatc 51720 taacgactaa aaattaaatt taaaggagaa tgatataaat atggcgaaaa aacaaaacac 51780 tactaaagaa ccagttgaat tgacacaaaa gattgatttt actgaatggt tgaaaaaaca 51840 ggaagcaacc tttagaagta aagtttataa agaacataaa aaatctaagc ccgttaaata 51900 gggctttttt tcttgacttt atttgagata tatgttataa tataagtata ataaaaataa 51960 ggaggagtat cacattgtca gataaaacta atacagtaga cgttgaatat ttaacgtatt 52020 ggaaactaac tccaacagaa aaagatagat taaaaacaga ttatggtatt tctagaaata 52080 aagctaaata tgatattgat aaacatactg tcgtaccaca gccagtaaat gcagaagatt 52140 tagatttctt ccctggtgaa tttgtagctt cttttgttat ctatgaagga caaaattatc 52200 cagtattttt aaaaattaat gatcctaatg aatacgttga tttaccaatt gaatcaacac 52260 caaaaggaga tgagctaatt gattaatgac aaatacgcct ttcaacaaga aatgaatgcc 52320 cgcacaaaat tagtatttaa aagtcctatg gatggaatta atttctatga tatagctttt 52380 gttgaagcta aaaaacttga taaaaagttt aaatctgaag gaaatccatt aaaatcttgg 52440 gatgatgaac gattattgtc gttttttgaa gaaattgcta atagatatga tgtaaacatg 52500 gggtagtata agctaccctt tttttgattt tttatatata aaatgatata attaattata 52560 aagttaaata agaaatcctt cctttttcat aaagagtata agaacgtgta aaaggagagg 52620 gatttatatg gctataaaaa aagattataa ttggctttct agtaaagcta ataaggaagc 52680 cctagaagaa aaacttcttt tctttaaaga aaaaacaggt cttgatatca aacattatta 52740 tgacaaagga aatttttgtt ctgggtttgc tcacgatcat gtagttatag aagcaaataa 52800 acaaattttt gctatctctg acgataaaac tatttatatc aatagaaaga ataattggga 52860 ggtttttaaa tatgaaggtt agtttagaat taaataacgt agaaccagga ttgcttgatt 52920 caattgtaag attagtagga gataatcaag tagaatttga tatgacagaa gaagtagaag 52980 cctccttaga agaaatcatt ttagatccat cttatttata tcaaattgat ggagaatatt 53040 attctattgt agatattgag caagaagtcc aaattgttgt tttttctgat gtatatgcga 53100 atgttcgcac gtttttctta gatactttac aattttataa catcaaaaga atggcagacc 53160 caagtttatt agataagttt ttagaaaaac aaattgaact tgatagtgga ctagaatgaa 53220 agtaattaca gacccagtaa gttcaacgcc atatatagtt ttgaaagaaa atcccaatat 53280 tcttgctctc ttggttttta ataaaaatgt ttttagcgat ttagatatta tatatttaga 53340 acctgagaaa gttaaacatt ttcataaagt agaattaaat gattttttca tagaaattat 53400 gaaatttact cgagaacgag aaggtaagat agtaaaaagt attgaatcgt atagaatttt 53460 gccaaaggag gataattaat ttgagagaat atcttgattt tattatgtat aagattaatc 53520 atttaattgg accaatttta attggaggaa tagcttttgt tgcattaatt gcttttgctt 53580 tcccacaact agcttctatt actgtatttg gatatgcttt agtattttta gtaggattga 53640 taatttcaat gttaggagtc gctttagcta atacaatata ttttcttgta gtaaaacaag 53700 aacaagaaaa agaaaagagg cgaggaaaat aatgagagat ttagatttgg aaagcctaga 53760 ggaacataag cccccacgga caattacaat tcctgatgga atcaatatta aacttgattt 53820 ctttaaaaga tatgaagagg ataaaaaacc agttatacta tcaaaaaatc cagggttgag 53880 cgaattagaa aaaatgttta cagatcctaa tacaaaaatt tatggatgga aaaaagacta 53940 gattttttct agtctttttt attttgccaa tattctttta tacctacatt aatttcttct 54000 tcgtcaggaa tatagatatc cccaaaaact tcttcaatag ttggaaaatt acttctaatg 54060 atttcatctt ttacttcaat aacagcatat accttatatt ctccaaattt accttttttc 54120 ttttgacatg ctaatttaac taagcccaat ttatctagta aatctaaaat agagtccata 54180 gcgtcataag ctgaacctga agattttttg cctaatatct cagtaattaa ataacgtttc 54240 gtaaagaaga agattttgtt cttatcttga taataccatt gcattgctcg aagaaaagaa 54300 tatattgtga taacattatc tgtttgtatt tgatatagat atttaagtgt ttctggatga 54360 atagtagtaa ataaatgttt tataggaagc ttcattttat cattttcttc caaaataaaa 54420 ccgtattctt ttaacttatt aatttgacga tttatagttc gacttgatac acataattca 54480 ttcgcagcct ctcgactaga aaagtctttt ttattaatat atctcggctc tccttttttc 54540 ccattccatt tagaaattac ttgtaaatat ccatataatc tatcaatatt tcttaaactt 54600 gtaaaatctt gtgtcgttgg aatttgattt ttatgattgt tcaaactttc tgacctcctc 54660 tctactgaaa tacaaaaaaa ttaggacagt ttgtcgtgag atacccattt gattttttat 54720 atatagcatg atataattaa ttataaagtt aaataaggga gggcaaacag taaatgatat 54780 tttttattat agtatttgct gcaatatttg tcaatatgta tcgacgcgga cgaaaagtaa 54840 ttgataaaga agaccagggc gtaacttttc tattttctgt cgctattggg agtatatttc 54900 aagctaccgc tgtttcttta gtggctatat ttttaacatt aggctattat aaaataacta 54960 atagttatga attatctatg gtagttaccc cattgacata tattaattca gagcaagact 55020 atattgttca taaaaatgga gaatcagatg aagttgattt ttatactttt gctacattag 55080 aagtggatgg gaatacaaaa ttatataaaa acatagaaat gaatgaaaat actgatgtta 55140 ttatagataa tagaacaaaa agacctaggt tagaaagagc agtgggttta attccaacaa 55200 ccctatggac atatattata agtttaaatc gtaaagaagc tactactatt agttataaat 55260 taatagtacc agaaggagct attatagaaa tgcatgaaca ggagaaatta gatgcgggta 55320 gaaaataagg caattaaaga actaattaaa gaaatggatc aatcatttga agagtttgaa 55380 aagaatatga gttatacttt atctgcaaca cctcttattc aagaaattaa agaagtaata 55440 gatcagcaaa aagaaaccca aaagagcaag aaaaggagtt ggcgtgattg gttaaagaaa 55500 ttgtggcatt aattactgtt ttgtttttag gtatcattgg tggttttatt attttgttta 55560 gtatagttgg aacgcttatg ttggtaccga ctaatgctat tggaatggtc attgttgtta 55620 ttgttggact agctatgagt aaattatatt cagttgcagt tcaaaaaatt atgccttata 55680 taagtaataa attaatcaac taagagtgat tcttagttga tttttctttt ttaaaatgat 55740 ataattaata tataaggaaa tgaaatggag agaagaaaat gaatttttta gattttattt 55800 ttaaaaaatt aaaccctaaa gaagcacaaa ttaaaaatat ttctctaaat gaagaaggca 55860 gagatttaat agttaaatta tatactgaaa tagaaaatga aaatgacatt gtttttagaa 55920 aaatatttca ttatgaagta catttattag aataaggtga caatcttatg ttattaaaga 55980 tattttgtgt gataggatta atgttttgtg ggtatattta ccttatgttt acagccgaag 56040 aatttcttaa gagaatacgg aaacgaccat atcgtagaaa gtgagctgat tctataagtg 56100 atagaatttc tattgttaat ctttattgct attatcgccc cgacgtttct cttaatatct 56160 ttacttcctc ttggaatagc atttatttta atgaatttag tgcatttctt tattttaaaa 56220 gcaataattt taggaatagg tttttattta acaataaagt taggaaattt aatggggatg 56280 attttatttg taggatgaat tggaggattt tctatgataa attgtaagtt tcataaagga 56340 gatataattg gttctacgac tgaaccaggc aagtattata ttattacaga aattaatgtt 56400 gttgacaaaa tatatattct gcgaaaaatt gaaatagatt tatttgatga gagaccaact 56460 tttactgaag gcttaatttc agtaattgat aaagattttt atagagtaat tacataaatc 56520 aataaggagg aaaaataaaa tgttaaaaaa taaaatgtta atgattagtg gttcaagagg 56580 tagtggaaaa acttttacgg ctttattata tgctagtaaa atggcttatc ctttggtggt 56640 tagagatgaa cgaatggtta aatatgtaag aaccttagca aatgcaacga atattccttt 56700 aactaaggaa ccaattacct ttaaagaata tgctaaaatt tgtaaaagag aatttggtga 56760 atctaatgat gagttagagt ttggttatgt agttgatgaa cttcaagatt tcgaacgatc 56820 tttttctgat gttaaattac ttaattgtca agcctataca tgtactttag tagagagttt 56880 tttcaaaaat gaaaaatgag ggaaataaaa atgacaattg agcagataat taaacaatta 56940 ggatccctac cagaggacga aaagaaatta aagttatatg tttgtgatat gaatggagac 57000 aatttcgcta tatgatgaag aagctaaaca tagtcctact aatcctctgg gtcttaatta 57060 ttcaatcaaa agaggtaatg aaaaatgaaa tatgtagcaa tttttatgcc agatttgctt 57120 tatatggacg ataaagtagg ggacttccat ttcatctgga atccccagac aagaatgttt 57180 caccagcgtt gtgattttga cacaacttat ccaccacttg ttattctcga aggttctgat 57240 tggttattat caaatttctt catttagaat ggattaccca aaaataaaat ttaaatcatg 57300 aaaaagaagc cacaaattat aatagatagg aagaattaaa atgtatattg tagaaattat 57360 tgatccagcg agagcagagt tagcttatag tccaaaagtt ttcagcactc aaacagaagc 57420 agaagagtat ctacaagaag cttgtggtaa tacgcatgaa ctagaaggcg ttatcgtatc 57480 agtattacta gaaaagaatt tcagtgataa tcgaatgatt ctggtgtcag cggatatttt 57540 ctatcacacg taacagaaga ggatgataaa ataatgtaca ctattatact aaccaaatcc 57600 aatggctatg gcggtatcac agaaacagaa tataacaata ttggtaatta tagaattaca 57660 gacgattttc taattatgga agattctaac atgggcggtt cgtactatat accaaaagat 57720 caaattttag aatttgagct aaaccgacag gtataaacaa tgagctatat tggaaatttc 57780 tcgagacata tttctcgcac atgaaagcac ttttgaagac atatttcgca actaagtaat 57840 atatagcgtt cccttcggga acctaaataa tagccttata ttttactcac ttcgttcgta 57900 aaatataagg tactgggcgg cttcgtacgt ggtgattttt atttgaaatt tttagtttta 57960 cgtgttataa ttaattataa agttaaataa ggggttgaaa atgttgagga aatatttcat 58020 tgattcacta ttaagcagtg tgccacgagt tcgtggtaga aaaagaatta ttttttatgt 58080 tgatgatgat agtgcattgg aagaagattt attagaagcc ttgcaagaat ctaaattcga 58140 tgttaaatgg caatctggtg cttctccaac actttttagt gctattaaaa tgaatggatc 58200 agttgcgatt ctttgtactg aaagagaatc aatggataac tttgtattaa ctttttgtga 58260 tttatattct gctttttctt gtgaagagaa agaagtctct cgtaaacttc agtatgagct 58320 ttttgctaga gcttgggggg taaagtaata tgagcatgtt acaatattat ttatcaaatt 58380 tattgaagag atgcagaaaa gatggcattt tttatgtgca atttgatgta ggtaataata 58440 gaagggtaat ggtgaaacta ttagcttcgc ttgcatcctt agaaggttat gatattgatt 58500 ggggcggctc aggaatgcct cttacaagtt actgtcctga gattgcgcaa ataattgttc 58560 tagttaaaga gaaatcggta tttacaactt gttttatcgg tctcgatgat cattctacct 58620 atgttccatt tcatttaata agtaaagtat tggaggaagg ataatggcaa ttaagatagt 58680 aggccccatt aaagaaataa cggaaaatga aaatcttata ggttggattt ctcctacgat 58740 ggatattttt agggatgaga tttttcaaaa aggcgatttt gagaaaacaa aggcttggag 58800 ggctaatata gagacatata ctcctagggc tggtggctat tcttttgcaa aaagatgggt 58860 cttggacaca gaagatatta ggtctgattg gttcaaacac gagtaaaatc ctccgttttg 58920 gtaaaaaaac aaggaaaaat aaaaaagaaa aaatccgtgt aatagacggt taagacgcac 58980 aaaacctagc ctagaggaca cattagacgg gaaaatatac gaaaaaatat tcgagagcgt 59040 cgaaaaatct taatattcga gagaaaatcc tcaatattga aatgaaaacc ctcaatcaaa 59100 aataaaaacc ctcaaaaacc ctcaatgttg tgtggcaacc cccagtcaca gtttaacgca 59160 aggctggccc cgtcctccct tacagcaaac acgacgatac gcgacaaacc tagaaaggat 59220 gatttatttg agtaaacagc ttaaaatggc agtaatccac ggaggtaaaa tggtatgcta 59280 ctttgatgat ggcactagtg aaactcatcg ctccgacgaa tggaatgtag accagatgag 59340 actccatgca attacacagg gcgccacctt cgtcagtgca ttatcggccc ccactggacg 59400 catattagaa tattatgtta atgataagcc aaaagtgcag agtatatcta tgagcgagct 59460 aacagatccc tactgggagc cccaaacaaa gagtaacggc cccactatat aaagaacgac 59520 aagcccctac tcttctctct tgggtgacaa cgtttgaaaa aatatattca taatgatata 59580 attaatatat aaatgagata tgaaaaataa ataaaaacat agggggcgga tatgtccccc 59640 tagtttactc ccccagtttc gggaggagca tttaggattt tcaattttcg tttttcaatt 59700 ttgaaagggg attttagatt atgaaattat cacgactaac tcaagaagat gttattaaaa 59760 ttatagaagg tactccaaaa gcacaagttc aaagaaaaaa atctggagaa ggctttacgt 59820 taggattaat ccttaataaa ttgactcgta atatggtagt agcagaaggt caagaaggtt 59880 tttctactta tcgagctaat gcagaaggat taatcactgg aaggaaacga tatagtgagt 59940 ctactggttt ctatattatc gctgaaaaag atgttgatgg acatcacatc aatattagtc 60000 aatctgataa tatctctaat ttaatcttgt atcctcaagc ttatactcgt gaagaagcta 60060 aagtaattgg aatgcgtctg cgtggagtag tcaatgattt attatcttat agcactgatc 60120 ttacagtaga aacaaaagta gatccatatg caattattca aaaaggatta ttaaaatatc 60180 taatgcctaa agaaagtgga gtcatttcag gcgcccatca atatatgcac aataatttaa 60240 cagacaaaaa gattggagat atagttagcc ctctctatct atctgaagaa gtacgtgcag 60300 gagctccatt ctctgttaaa aaatcattag aaaagattga ccctgacttt aaactagaga 60360 gtcagattgg cttatttgca atctggtcat ggtcacaact atttacttct atcgaggaaa 60420 atggttgggc aggttcacct gtattaaatg acctaggttt tgatcgagac tttatccaag 60480 aaacacttaa gaaatttgat gaagacgtta aaaaacatat tgtagagatg caatatgcat 60540 atacttctca gaaatatatg ggacgtgaat acctacgtga taatctggat caatataaaa 60600 cccctacaag aatcagttca tttgagtatg gaatgctagc tacctatagt ggcactacag 60660 gagcctatac tttggtatct attcaaggca aaaatgcatt cactaatcct tgtcttatgg 60720 caggtttcct tcaacctgca acctatgagg aagcaattga tgatctaact attggaaata 60780 ttatttcaac tgagccttat ggacaaaaca aaattatctt tgaaaaagca agtactagtg 60840 tatctcgtta tatcttagac aacttaaatt ctactgaaga agaagaataa aggtaaggac 60900 ccctcgtggg tctttttttt gtattagaca agataattta tcctctctgt tttattataa 60960 cattttatac agaaaaaatc aaggggtgct gagttttagg aaaaagtcct ggctttacga 61020 ggcaagcctt taacggggaa aaaatctagc gtggtggtcg acattaggct gggttccggc 61080 cgtgcacgac cggccaaaaa ataacgtccc cttatgagag aattctaatt ttcacaaatt 61140 aaaataacag ttttgggatt ttgggttttt attcatgtta ttgaatatta attcagggag 61200 ctgaaaagcc catcatatca acgttttggg gcggtgttat tttataaatt aagttttttc 61260 tgttatttta tgttttttta ataacaagag aattttaatt tttcacaaac tattattaaa 61320 aaataacaaa aaaattatta gaaatctcta attttacaat tttgagaaaa atttgatttt 61380 cagaaaattt catgaaaact ttcaaaaata ttttcatttt catagattaa aaaatagtga 61440 gatttctctc accattttct catttattga ttttgaaaac ttgcttcaaa atctttcatg 61500 ttaatttcag caattttttc agaatgaact tttgtaggga tacgccagca cattctattt 61560 atttttcgat tcattttttt atgttgttta taacaccatg ccataatttc cgtttctata 61620 tatacatcag caagccctgt atgttcttcc acaaaatcgg ttgttttcat catccaatta 61680 taagcgactt ctgcattagt tttcagattt ccagcttttg agtaataacc attttcaaac 61740 gcccactctg gaaagctctt ttgtgaaaaa attgtctggc aagccatatg ccaaatgcaa 61800 ttgatttcta aattttttcc aacttcataa ggaaagaaaa acggatagtt tgtcaattct 61860 tgccatgtgt tatttaaagc tttaacaaaa tcaaaatacg cgttgtatgc tgctacggtt 61920 ttgatattgt atttttctat aatgttaaga ataacttttc tcacatatct gaacggcttc 61980 acttccataa ttcctttaga aattttttca cgatacaaag gtaattttgt atagtaataa 62040 gcgctacgca tcaaagcatc atcgtcaaaa atttctgaaa ttgcgaacga atgttcttcg 62100 taaatattcc cttttttatc agataccaca aagccaacat cataacataa tggatttttt 62160 tcaacgtcgc cagctgtttc tacgtctaaa actaaaatgt atttttttct tttgtcaatt 62220 ttcatgctta ctgtatccac ctttttaata aaatattttt ttgaaaaaat aaaaggctag 62280 gcttttacgc ctagccttag acgttcctta gataatttta tagcccactt ttttcttgtc 62340 gcttgttgcg attccttttt ctaggactcc ctcctcaact agacctttta ggcgtggggt 62400 cattttttga ggtgtatagt caaactctaa agcttcattg acttcttggc ttgtgtaaaa 62460 tgtttctgga tcagcttcct caaaaaacca ttcttgaatt ttttgcatgt ctgcaagttt 62520 ttctttgttt gtcttgcttt gactaggttt gcggttggcg ttacgttttc gagcttgttc 62580 gcctttttct actacaaaat cgataacttc ttgcggtgct ttcacgtttg acaagatacg 62640 agcaacctct gcaaattttt cagctacgac cattttttcg aattgtgttt tttccatgat 62700 aaattccacc tttttaaaat tttgttagta agttgttccc ttactactct ttaagtataa 62760 acgattttca attattttgc aagtcttttt ttgctttatt agaaaattct catttaagct 62820 taaagagtaa caagtgaacc tccctctcaa gtataccata ctttttttaa ctttgcaagc 62880 ataaagttta tttatttttc aaaaaacttt ttacgtcttt ccttatttct ataatactat 62940 tataacaaac ttgaaaagaa attgcaagca taaagtttat ttatttttca aagaactttt 63000 tgcaatctct ttccttatct ctaatactat tatagcaaac ttgaaaagag attgcaagca 63060 taaagttcta aataatttta tcaattgtga tttttttgat tagtgtttca agtgtttgtt 63120 tggttggttc gactagtaaa cgatagtcag catgtttgaa taaaccgaaa tcgctcgcag 63180 tatctcccca cgcctctata taactatact cgtttgtgtt tatgttccat tgaatgcagt 63240 cgtctttttg tgattctgaa aacattccct taacttttcc agtaaaccga tcatttgttt 63300 tcaaatattc cgttgcataa tatgaacagc ctaaaaagtc agctaaatgt ttaactaaaa 63360 aatcgctcga acctgtaaca agaataacct caaagccttg taaaattttt tcttttaaat 63420 cggtcaaagt ttgataccaa tttttttcat tttctaaaaa ttgagaaaca aactcttttg 63480 catacatttc tttttctgtt tttcctgtta tctcattgcg ataattttcg gcaaccgcaa 63540 cgatcaaact ttcatttttg acatcttttt tccatgcttt ataagaaccg tcgtccttga 63600 taattccttt ttcaattaaa aaacccgcat gagataaaac aacgcttttc cttgtaagtg 63660 ttccgtctac atctgttaaa attaaatttt tcatttcttt cgtcctccct ttatcttata 63720 tacttattat aagggagaaa cgacaaaaag taaagcattt tatacaccaa aaatcattaa 63780 agtttaatga gaattgtttc taatttacac acaattcacc aggcgttatt ttataattaa 63840 ataacagctt ttcttcggac acatgttatt ttataaacct ctaagcgctt acaaactgcg 63900 tcagatcaag ctttcaaaag attttcagaa attttcattg ttgtttcaat aacaagcaaa 63960 aattacacgt gaaataattc acaaacataa aataacaccc aagagcagaa aaataacaca 64020 ctctggcttg tgcgcacatt cacattctct tgctctcttg gaatgaaaag gctatgaaag 64080 cttgatataa taggctttct ggattgtgaa aaaattcaca tgcatggact tttgggatat 64140 taaaataaca ttaaaaaaca attaaataac aagccaaaaa cttgacaggg ataaaataac 64200 acagtaaaat ggcgctcgcc agcgagcggc cgaaaaataa cgtcctctat ttgttatttt 64260 attcatttca caaactttag tttcaggatt ctgttatttt atttttgaaa atcctttttc 64320 aatttatgaa aatggaaatg aaagcttgat agatcaaggt tttgggaacc tcgccccaca 64380 atgttatttt atcttattcc ttttgggctg ttattttatt ttatacattt cacaaacttc 64440 acttttaaaa taacacgtat ttctttgtga aaaaattcac aagcaaaatt aaaaaaagat 64500 gagaaaaatc tcatcttttg tttttatgat aatcactaat tttttttagc tctgcggtgt 64560 taataatata ctcattacat aaatccaaaa ggaaatcctc aaagctttct ttttcgcttc 64620 ctcttgtttc ccaaagtctt tctaattttt gatacatctt tttattactt aaaataatca 64680 tttaaattca ttcctttcct ttactatata tatagtataa catgtaagta aaagggagtc 64740 aacccttttc ttcaatttct tttatgattc ttttttcgtc ctctggtaac atttggataa 64800 cagtttttac tactatagtt ttatctgttt cattacaaac atataagcga gaaccgaaaa 64860 cccacaattt aaattcattc tgttcatttt ttggcgtttt ctttcttacg ttcttaattt 64920 gaaaatcgtt aagaattgcc gaacggactt gcctattatt cattggcttt ttggctactc 64980 tttctaagta gcgttctttg gcgtgttgtg tgataataac ttttttagta aacaaaagca 65040 tttgatccaa ctcctttcta tattcttatt atatattata agggctgaaa agtcaaccct 65100 tatatttttt taattttttc gtttgttata attttttcca tatttttttc tacttcaaat 65160 acatggaaaa aattccaaag cctatgattt tctgtgttat cataatactt cacaccgtca 65220 aaaaagcagc acaattcagt cagttctgta gctttttcat catttaaaaa atcaattatt 65280 ttataaatat attcattgtc aactttaaat ttgttttttg aaacaattgc attcctaaaa 65340 taaaccttga ctgttaattt tttagtaatt aagtttagtt tcgtttttat gatacaagct 65400 tcatctgttg acttttttaa atataatttc ttttcttgaa atgttttaaa aaatctcatt 65460 ttattcactc cttattcttt atattattat tataacaggt ggtaaagggt ttgtcaaccc 65520 tttaccttat attttttagg acttccgccc aaatcttcta aatatccctt ttctttcatt 65580 tttttcaatc tgctaggtgt ctggcggttt gtgtatttat cagcaactaa caaatgaaag 65640 tccttatttt ggaaatactc tttcccacca aaagcaattt ttactacatt gtgaaatagg 65700 tcatcaattt catcatctgt aatttttgat tttgcatttt tggctttctt gatttctttt 65760 tgttcttttt cgaagtctgt ttttttgtca ctttcaaact tatcgaactc tctcagctca 65820 atagcttcgg caagattcaa ctctaatttt tcaaccattt catcaattaa tttccaatca 65880 tgagaagtaa tcttttcgcc attagaaaat gtttcttgca tataacacca ttcctttcct 65940 ttactatata catagtataa catgaaaatt ttttcgtgtc aactattatt ttttatttta 66000 tttatttttt tgttccctcc ttaactttct ataacaatta taacagataa aactaaaaaa 66060 atcaaataat aaatacatga gttttaggat attttttctc attaaacttt tagaaaaatt 66120 tttgattttt ttcttgacat agacctaatt ttgtggtaaa atttccgccc gaaaacggca 66180 aatttgctca ctatttcaca agccttcaac acaaaaaaag atagcttttt taagctatcc 66240 taaaatataa tcataccaaa gaaaaagagt tatgtcaact ctttttctta tttaaatttt 66300 cttcattcac aattcgccac gcttgagggt taatagaata ggcatgaata ggaatttctt 66360 tttcttgatt taatagaaaa tgcagaattt ttgtttcatc tatgctatct tgcaaagcgg 66420 tatgttcttc aatgtaattc gtatcattga acaaaaattt ataagttgct tctgcaccgg 66480 tttggtaatt tcctttttct gttaaaaggt tgtgagtttt tgcaaactct ttatactctg 66540 gcgtattcaa tacagtataa gcggctactg gataaatatc aatcaaaaag aaattatcaa 66600 agaaatcaaa atcttttccc gaaaaaaaga aatgggttct tgcaattaca cgagtatcaa 66660 aacctaaatt gtaagcgcca atgtaagcaa cattatattt tttacaatct tttttaagtt 66720 gtccgataat gttcgcccac ttgctaacaa tgattctttt ttcaagtaat tttgtaaaat 66780 agattttccc atcaactaca ttttcatcaa ttaagaaagc acgtttttta tttaatgcct 66840 gaaatttgaa ttgttccacc atgtaagaac gttctactaa aatatttccc cctttgtctg 66900 aaataaccca accaatatca aaaactgact gataaggcat tccctcgccc tcttttgtcg 66960 tttcagtatc taaaatcaaa aatgtttctt gcatgtaatt ttccaccttt tcaataaatt 67020 tatttgttcc ttaactacta tttaagtata acaaaagggc ttgagcttgt caagcccttt 67080 tactaactta tttcaatttg taagctactt ttttatcctt tacaactttt tcaaattctt 67140 cgttgtcgac taatggcttc atgattgccg tcaatttttg aggtgtagca ccaacaagcc 67200 caattaattc agaaatttcg ttcagtgtca aggctgtttc tggttctagg tcaagaaatg 67260 cttttcgtac tttgtccata ttttcagctt tttcttttgc tgttttgctt acgccttttt 67320 tgccacgttt tgccttgtct tgtttcactt gttgagcgtg catagcttcc aatttgttta 67380 gtaattcaat atcaactttt tcaaataagt cagtatttaa aaaagttttg tcctcgattg 67440 ctttgcttac tactttactt gctaattcca atgcttgtgc ttttgtgatt tgtttttcca 67500 tgataaattc caccttttta aaatattttt ttgtaataat ttcttattac attcttagta 67560 taaaacattt tgatttgttt gtcaagtgtt ttatactaat ttctaacaag aaatttttaa 67620 attaaatcat tttcaaaatc agaaattaaa ttatctaatt cagtttcttt atactctttt 67680 ttatttttta aagattccaa aataagaact tgccacaagg ttaaatctgt ttgtgtgagt 67740 tgactacaat accaacaagc ttcttttaaa gagaaagttt tttctttcat ttatttcaac 67800 tcctttctat actcttatta tagagtattg agaaaggctt gtcaagcctt ttcccaattt 67860 tcttttaaaa aagtaacaac gctataactt gtaatagttg ttagagcaat aagtagcaaa 67920 ctttgaatag agaaaaatcc agtatataaa atagcttgat aagtaaagta gaatggtagt 67980 aagatacctc cagcgaccaa aattcctaca aaaacaatta ataatactaa ttcagataat 68040 ttcattttaa aacttccttt ccttttctat atattaagta tatcattact aaaatgtttt 68100 gtcaatactt ttttgttaaa taatttcaaa ttttctattt ttgtattctt ctttgaaatc 68160 ttgaacagct tgttcataag tatcagcttc aacagaaatt tcttcgattt taggataacc 68220 actatccaaa ctaatttcat ctaagaattt aacaagtaaa atatatttat tcattttttt 68280 catccctttc cttatcttga ttctattata acacgactta attcatctgt caacactttt 68340 atcattttat cagctttttc ttttcggtca atttcttttt ctctttggtt ctttctagca 68400 atcaatttca atcttaaata tttatttttc atttcaatcg ttcctttcta taattatata 68460 ttagcaaacc ttagaacttt tgtcaagcat tttattaaat ttattttttt tattttattt 68520 tttttgttcc ctccttaact ttctataatt attatagcat agaccaagag gaaagacaat 68580 aataaaatac tgtattttta attagaattt tctcattttg tataacagat gaaagcgctt 68640 tcattttatg gttccctccc tgtttgcggg gtgttctcac ctctccttat tctttatata 68700 tttattatag catgagtttt gggacaagtc aacagaaaaa cgccaaaata aataaaaata 68760 ttttttcaat gaaagcgctt acatttcttt cctctcttgg tacaatatta ttatagcaca 68820 aaaaaaggac ttttgcaagt cctttatact aattcttttc cgattatatt tctgtctaat 68880 acgtcaattt tttcttttac ttcttcatta tctaaaaatt tatttaagtt ttcttcattg 68940 aaatttttag ataattcaag agctaaaatt tcactatctc tcaatggata gttataattg 69000 tttattcttc caccaactac tggaaaacat acctgttcgt tagtatcaaa atctactaaa 69060 tatatatcta aaattgaaac attattccat gcaaatgagc cactagcgcc agcattcctt 69120 acaatttttt cggaagcttc ttgatttgat cgaaacatta ctactttagt aactcgatta 69180 tttattgttg tcactgtttc ttttctatcc attaattttg gatcatgatt gtatattctt 69240 cttctgtacc acgcttcact aatactgtaa ccaattataa acaacatcat acctaatata 69300 acaattaaca taagactacc aaaaattatt ctcatttaaa aatcattcct ttcttttaac 69360 tataattagt ataccatagt ttcggggtgg tgtcaacaac tattttcatt taaccattta 69420 acaaaatcat tatagacttg atgaatgtca aatctatttt gaaattgcat actaaaatca 69480 tatttgttcc gtaaccattc actaagtaca tagctatcaa ctaaagggct attaagcttt 69540 ttcttatatt tagataggta ctttcgttct aggtcatact ctaaagcaat ttctaaaatc 69600 tcaatagctt caagagattc attattcatt tttaaacacc tctttccagt aagttttagg 69660 gctattcatc taacccctat aaatagtata atatataaaa ccctaattgt caaatgattt 69720 ttaacattag acaaaatatt tttatataca ttatgtatgt atatatgcat ttgataacag 69780 atgttatttt aatagactat cgatagatgt aagcgcttac aaaagattta cttaaagaga 69840 tgaattgaaa gcgctttatt ttgcgtttta agggcttttc atattatttg gcataattat 69900 ctattaagca ttataaaacc caagagaaga gcaaagaagt gtattgtggg cgctattatt 69960 gtcttttatt aaggtgctga aataaatagt cttatataaa ataacattta tcagttatta 70020 taacaagtgt tatgtttggt gcagagatgt gatgaactaa tacattgtga aacatttcac 70080 acatgtacaa tcattcatat aataatttat tcatatgtat agatgaacag atgaacattt 70140 attcatatgt actcactgct agtctgtgag cattgtcaca gatgtaagcg cttacaactc 70200 actgacgcaa ggtgtcaagc aaagatactt ggcgcacata tgttcggttc gttcccagca 70260 acttgatgaa taacaaaggt tatttaattt tatggttcgg cgtttaaaat aacaagtgtt 70320 atttaaacat acttaaatac ttgaatagat atacatatga acatatattc atatattgtg 70380 gcagtaggta cagatggact agaccaatca caaccataaa cgattgtgaa atatttcaca 70440 aggattccac tcaacgttag aaattagttg atattattaa ataacaggtg ttatttttgg 70500 tggcagcaac gcagatgaat gttatttaat aaacaaggga gtaggtggca aggggtatac 70560 aggtcgttat ttaactaggt caataagttt ttacaatagt ggtaaaaggc ttgttgtcat 70620 tagggtttgg gcgttttcag gaatggaatt gagattggaa ttggatttac aattgttatt 70680 tccgcccatg ggggttgggt tcatgcgggt cttacaggct gttatttttt ctgaaatagc 70740 ttttggtggg gatggttttg ggatttgtga aaatattcac aagtcaatca gggaagccgc 70800 ggcaacaaca cttaatcaga gcttcaaaat tgaaaatcgc atgactgtct tttttctacg 70860 gcgtctcacg gttggggtag atttatctat agttctgccc agtttttata ttgataccag 70920 agtaaatgta aataaaggag gagttcgaca tggcaaaaca cgtcatagaa gaagagcttc 70980 tgcatggttc gggctttaac gtcgtaacta atggagaagt agaaattaca gctgataacg 71040 ccgagcggat tttaaaattt attaccgatg gcacaattga tattgacgtt attaatcata 71100 atattaaatt tagctctcga gttccagtat tcacaacatt agattggtaa aggaggatgt 71160 gaataaatga ttaagtttca caaggttagc ggcaaacccg ctaaagtaga atctggcgat 71220 gaaaatattt ggttttctta tccaaatggt atttatgttg ctgataatca aggtaaccca 71280 cgattaattt ccccagggaa aatgattggc gatgctgttc ctgtgggagt accaaatgag 71340 ccaggtctat tttattataa tacatcaaat aaaaagaatt atatttccac cgccgaaaaa 71400 tgggttgaaa ttggcaccgg cagtattcca gggacaggtg gaggcagcaa catcgctggt 71460 aatgttttaa tcaatgatac cgccgataat tatacctcgg tagatgttga aggcgctctt 71520 gctgaaatcg ccgaaaaatt cccatggtat tttggcagaa tgcaattaaa agattttaat 71580 ggtgacgtta atactctatt aaaatccact gaaaattata ttacaactac tgccgcaaat 71640 cgccccaatg gcaaaacagg ttggctcatc caacgcaagg cttctaataa caatacttat 71700 ggattcgtca ttggcgatga tggcaccgcc caaacaaggg tcaacgatag gttcacccca 71760 ctagcggccc aatcggacct tgccaaacta caaaatactg ttgattcctc tcttggggaa 71820 ttaaaggtaa atgtaggaaa gggattagct tctgatggaa agacattggc aaatggacaa 71880 actatttcat tagcacaaga tattttagat aaaattgatg ccgccggagc tggagctaat 71940 tttgttaaaa aagctggaga tactatgact ggttctctag taatgaaagc tggttctggg 72000 caggtttctc aagtacaatt tagtagtaca ggttctgcta ataatgtggc aatattggat 72060 gacccaagag gaacgtccaa gaattctcct tatggaggaa aagaaggcgg ttctcgtttt 72120 agaattgcta atgtgaggaa gaatgtcgat atcatgtcag cttatagcga tgggtcggtt 72180 gatattcatg cacatggtcc tcgtgcggta tttggtaatg taagtactaa tggacgaact 72240 actattttga ataataataa taatggtggg acttcagttg atatcgttgc gccttttagt 72300 aacgttccat tatttgttaa gggtaaaaat ggacagctac attataaggt taatggtgga 72360 atgaatagtt tgctttctgg taacagtagc ggtggtcgta aaaatttacg tattgctggt 72420 atgggaagta attctgaatt ggctacattg tattttgaag catggaatac aagagctaaa 72480 ggtaaaattc attctgggtc agctattaat gttggtttta accatgaaac ttctaagggt 72540 actggtgatg gggatgtatg tttacgtatc ttcccaagaa aagctgggct taatggtgag 72600 agaaatgctt tgagaaacta tgctgtatta tattatgagc aatatagtca aactttagtt 72660 gttgactcat tcaagggtaa tgcaaacttt acaagcaatg aacgtaataa ttttgtacgt 72720 ttatcttctg atggttttat tacacgttct tctgtaaaat ataagaatcc tattaagaaa 72780 tttgaagaag atgctttatc aaaagttaaa gaagttacac ctgaattgta tgcttataaa 72840 aatgatccaa aacaaagtac tcaattagga tttattattg agcatggtct tccccaagag 72900 gtagtagaaa tggaagggcg aggcgtgaac gcttatgcgc tgacagctta tttatggaga 72960 gcgacacaac aactgtctca agaagtagat tcattaaaag aagaattgga aaatttaaga 73020 aatagttaag attaaaaaga gaacttgaaa aagttctctt tttttacttg acattattta 73080 gtctatatga tataatataa atatagaaag acataaaata ttggaggaat aactatagat 73140 ggcatcatta caattaaata atgctttaca aacctatgaa gaaagattag aatgggttaa 73200 taaagagatg aaaagaattg gagaagataa attaactcca tatcaacgag ataaaattgc 73260 tacatattta atagatggaa ctaatccaga atctcgtgga gaaattatta cagctaatcg 73320 tttagttaca gtaaataaaa gagaaacttc aagagaagga ttaatggaaa agcttgaagg 73380 aggagaaagt gctttccatc aattaattaa acaagataaa aatgtaattt taacaccaaa 73440 ggtagaaata acagaaaaag atattgaaga agtacctggt ttggcacaac taagagaaga 73500 aattgataaa ttacaaaaat atttagatga aaatccagat atgcctaaaa aattaacagg 73560 gaaaattaaa caaacaatta ttgaaatgcg tagagaccaa tatgttctta aaaattctca 73620 tcgtcaacca atttttggaa gaggtaatac aggaaatatt gaacaagata ttcattacga 73680 tactattaac ctacaacaac gagatcaagt attatcatta ttaatcaact atagtagctt 73740 aaaaattgaa tttaaagata ataatgattc agatattaaa tgggttttag aagatcttga 73800 tagattaatt caaaaatctc ttgctgaaaa acctacttta ctttatattt taaatgaaaa 73860 aattattggg tcacgcaatg atgaaataag agaaggactt attgaaattt ttggagtaga 73920 tcatacacaa gaatatattt catctttata tagaaataaa atcccacaaa ttattgcaga 73980 cgctgcaaca gaagagtgga ttgattatat gtacatgaac aaacttaagg gaacttacaa 74040 acgttgttct cgttgtaaag aaattaaatt agcaaataat cgtaatttta gtattaatag 74100 aacatcttca tcacagtttt attcaatttg taaagaatgt cgtaacagca aaaaataacg 74160 aggagttact aatatgacag ataagaaacg agtaattttg gatacaaata ttttgatgag 74220 tacaaaaaga attaacagtt taattgagga atttaaagat tgtgatttat tagttacact 74280 tggaactcta ggagagctag atagattaaa aactgctgaa ggtcagcgag gatttgaagc 74340 tagaaatggc ttaagaatgc tccgagaaaa tgaagaccta ttcacaatta tcgacacaga 74400 aaatccagaa agaccctctt atgataaatc tactgtagat ggggatatca tattcttggc 74460 gcaaaaagag aatcgtttgc tttccgagat cgaagtaata actaacgacc tatccatggg 74520 aacagtagct aaagcaaata gggttaatgt acgaaccttc tatgaagacg cagacaaata 74580 tcgagaagga tatgcagttg ttgatgtctc cgacgaatta gaagatgata caaacgcaat 74640 gctatttgcc ctagctaacc aatatatttc ctctcttggg ctattagaaa atgaatactt 74700 agtattagaa aaacatggag aaccttactt ctttgttgta gaagaaggtc gtgccacaaa 74760 aatctttgat aataatccag taagatacaa taaacatatt aaaatatgct cttctgaatt 74820 tggcacttta aatcctaaag aaaaagatta tgctcagttt tgtgcttttg atatgttagg 74880 caagcatgat atttcgctat taactggtcc tgcggggtca ggaaaaactt atttaatgct 74940 tgcaaaacaa tttgaaatgc tagaaaaaga agagatttgc aaaattacta tatttgtcaa 75000 tccagaaaag gctcgtggag ctaaaacact tggtttttat aagggtgata gaaatttaaa 75060 attgatgcaa gaatctattg gaggaatttt agcatcaaaa attggtgaac gagaaaaagc 75120 aattgaatat attgaatctg gacttattga aattattcct atttcagata ttcgaggtta 75180 tgaagttaca gaaaattctt ctttatatat tacagaagct caaaatttaa ataaagattt 75240 aatgcaatta gccttacaac gtgctggaga aggaaccaaa gtttttattg agggagatcc 75300 aacaactcaa ttggattcgt gggcttatga aggtgataat aatggaatgc ttaaattaat 75360 tgaagttttt gccggaaccg aagtttttgg caatattcat ttaagtaata tttatcgctc 75420 gcgtgttgca gacctagcag aacgaatgac aaaataatat aagacattat gtccagcttt 75480 caattattct gaagctggac atttttatta aattaaggag gtttaaaaat ggctcaggaa 75540 gaaaaacaaa agtcagtagt agaaatgcaa gcagaaaaaa ttcactgcga acattgtaat 75600 agattttatc ctgcaaagga tttttataaa gataaaaatg gagacccaat gaaaaaatgt 75660 aagaaatgta tggcatctct tattgattta aaatctccat caacagtaat gaagattatg 75720 gaagaaattg atattccata tattccagat gaatggaatt cattaagaga acgatacgaa 75780 ttctcaacat ctaaagatgg taaaactatt cgtaacaaaa atgcaaacca atctgtctta 75840 ggacgttata ttggtaaaat gcgattagaa caatataagg gttataaatt ctctgacact 75900 cctcaattta tggaacaaca cgatcaagaa attcagcaaa aacgtgatat acttcatcaa 75960 aaactagaag atttattaga caaaggttat gatcctgata ctgctatgca actaatggca 76020 gggagaattg atacagaaga tgaagatccc gcattaacta aaactcaaat aaaagaatta 76080 aaaattaaat ggggaacttt atataacgaa gaagaattaa ttcaattaga aactttttat 76140 ggggaaatgc acgaatctta tgatatcact tctgcttccc atgaagatta tttaaaacaa 76200 atctgtaaag tttctctacg tatgcacagt ttaattgatt caggaatgta tgatgaatac 76260 caaaaattat ctaataccta tgataaaatg atgaaatctg ctaaattcac tgcttcccaa 76320 gagaaggaag aagataaatt cattgatagt atctctgaaa tggtgcgttt atgtgaagaa 76380 gaaggattta ttcctgtata ccatacagat gagccacaag atatcgtgga cgttacattg 76440 cgagatttca caaactacgt acgtaattta gttgaaaaag aattaaactt agattctcta 76500 attgaaaaag gcttagaaca aattaaattg gacgaagaaa aagaacaaat gtctgcagat 76560 gatgctctat ttgtggaaga agacatgatt tctgatgatg atttattgtt tgaagctgtt 76620 aatactgagg ttctaccaga acttacggat gaggctaagt tattgggggt agagcttgat 76680 ggcgaagaat aaaaaacatg aagataaaag tacaactatt attgatatta aatcagattt 76740 tatcaactct aaaaaaaatc gagaagtatt tttaacagaa gagagagtgc gcgcaagcat 76800 tcctactttt acagaatatg tctccttttg gagagaatat ccagatatgt ttattgatat 76860 gataaagggt ccagatagca aatttaattt tttcttttat caaagattat ttttacgtgc 76920 tgctatgagg cataaatact ttttcggtac ttttacacgt gccttttcta aatcattttt 76980 atctgttatg ctgatgatgg aaaaatgtat tttatatcca ggaattaaag tatttattac 77040 ttctggtggt aaagaacagg ctgctggtat tgctgcagaa aaaattaatg aaatttgtga 77100 tatgatacca tcaattaaaa aagaaatcaa ttgggcgcca gggcaaacga agcttaatgg 77160 taaagattat gtacaggttg tatttaaaaa tggaagtcga ttcgacgtag tagcagctaa 77220 agaaagttct cgtggtggac gtcgacattc aggacttatt gacgaggtta ttttggttga 77280 cggagaaaaa ttctcacaag ttatattgcc taaacatatc tgggcctctt agtagtaata 77340 ctaaagagat aaggtggtga acctcgatta caagagggtg tccgaaaggg ctaacgaggg 77400 aaaccttaga atcttgatac aattctaaga taatctcgtg ccaagccttt gtgaagatca 77460 taaaggaagg tgtagagact atccgaaagg agtaagatgg agatgttacc atctgaagcg 77520 ccacccatcc taatttaaat aacaagagag gatgaagaaa tagtccaatt attatcaata 77580 attgatgatg aacgtatcac gccgtgcggc gaatgggaag gttgacccaa atgataaaat 77640 gaataaatct caagtttata ttaccagtgc gggttgacat acaagctctc actataaaca 77700 ctcactctaa ttgctgggaa atcctttata aaaggacaat cagcagcgaa gcctattaaa 77760 aatataatag gaacgttcaa cgactattcc gaaaggaagt acattcaagt gaatggaaac 77820 gggtgactcc tgaaaaggga gaagatatag tctcttctat atggtaacat atagcagttc 77880 ataagagaac gctgcaaaat taacgacttt gcgggaagat aaagtttaaa gatcatttta 77940 gttaccaaaa acaaattcag ctattagtat ggcaattaat taaacctggt accgctatta 78000 ttatgggggg aacttggaga actcctgtta aaatgggatt attagatcca ggttttgtca 78060 acgatctaaa gtctgatggt acttttgacg aggtatcatt taaccgtgag tgaatctctc 78120 atgctcacgt aaaatgcaat taattgctgg gatatcctaa agctttttaa actacaacat 78180 aagaagaaat tctaagtgtg aatgttaaaa attaaaaaga taaatggaca atcagcagcg 78240 aagccccttt cacatgggga acgttcaacg actatccgta aggagtaggg aaaccgaaat 78300 attgcataaa tatattaaat atgatataat catagtaaga ggtgaattac tatgaatgga 78360 aaaataatag atattgctcc aaattatatt atatatccag atggaaatat taaaaataaa 78420 aatacaaata aaatgttagt tggacaaata gggaaaaatg gctatcgttc agtgaatata 78480 agaactctta atggaaaaaa acgggtttat attcataggt tagtagctat taactttatt 78540 cctaatccgc atcacaaaga acaggttaat catattgatg gaaataaact taacaataaa 78600 gttgagaatt tagagtgggt ttctccgaaa gaaaatattg ctcatgcaaa aaaggaatta 78660 ggttttactc ctccaccttt ttctgaagtt acgagagaaa aaactaaaaa attaaattct 78720 aataaaatag ctaaatatga ttgtaaccaa aatttaattt gtatttatcc ttctataatt 78780 gaagcatcaa gacaagaaca tgtttctcgt catagcatta aagatgtttt aaaaggaaaa 78840 aaaggaaatc acaaaggttt catttggaga tatatttaaa gatatagtct agttccaacc 78900 gaaagtttgg atgtatatta gatgagagtg tttgggctgg ttctgcgaca gaatcattct 78960 ataatggaga cgttttcgat agatgtagag ttttaaaaca ggctgaattt acaagaagtg 79020 gtcgaggaaa tgatgaagct tattatgtac tcggagtcga tgtaggtcgt aatggttggc 79080 aaactgttat tacagtggtt aaggtaaatc ctcagcgtaa tggtgtagga attaaatctg 79140 tagttaatat gattgttatt gaatcggagc atttcggaat ccaagctaac gaaatcaaac 79200 gtcaatattt aaattatatg cctcgttatg ttgtagtcga tggtaatggt ttagggattg 79260 ggttggtcga ttatttagtt atgccttctt cagatagtaa aacaggagaa agtttttctg 79320 cttttgaggt tgttaacgat gataaaggtc tttataaaaa aattgataat catggcgata 79380 gctatggtaa agttttatgg attgttaaag ccgactcaga attaaacttt gagggttata 79440 ctgcactact tcaacaaatg ggttctggta aaattcgata tttacaaaac gagcgtgatg 79500 caaaaggtga tttagaaagt aaaaaagcat ataaaacaat gtcagcagga caaaaagctg 79560 acgctattag accttttgta ttaacgtcta cactaaagaa tgaaatgatg aatttaacta 79620 gacctgaaaa taatagcact aaatttagct tagatagaat taacaaggga atgggtaaag 79680 ataaggtttc atccttaatg tatgcaattt atgtaattaa gttacaagag gacaaagaac 79740 ggaataagaa gaaaagtagt cttgctaact ttgcattctt taactaacga aaagaggtgg 79800 aacaatgagt acattttctg gagtaagtag aggacatcta agaattcgtc atatcttaga 79860 agaaaatggt tttaattggg aagaagaata cagctttcct gatttagtcg cttctagcgg 79920 ccgccaatta gcttttgact ttatggtaat ggacgacgat ggaaatatag attttgctat 79980 agaagtcaat ggtgagcaac attatgaacc agttgctgct tttggaggaa aaaataaatt 80040 taaacgtcaa caatacaacg accatcaaaa acggttatat tgtcataatc atggtatccc 80100 attagtagaa attccatatt gggaacttga aattgttgat attggatatc ttttggaaaa 80160 agcaggtata taaaaactag aggccgatat tgcataatat cggtctttaa tatatagttt 80220 tataaggaaa gatttcattt tccaagagat aaaatagaga aaaggaggaa tataattcat 80280 gttggacaaa tcaaaaagac agagaagaaa gccaactcca gtcaaaaaag aagaaagaaa 80340 actttacgaa aataaaaaac cttcagtaga tttttcttat tatgctgaca gaagcttcac 80400 taattttggc ttcgggtctt atacttcaga attaggagaa tcatttgtaa caactgacga 80460 cattcgtaaa gctttggata aagcttttag acgaaaagat gttaaaggca ttaaaacttt 80520 gtcaagacat ttttatagag tttctggtgt gtattctcgt gcggctgaat atttagctta 80580 ccttccaact tatgattata tgattactcc tcgtgtagta ggcacaaaaa ttaatgaaga 80640 tactattgta cgagaagttg ttaatcaact attgttctta gaaaaagcaa aattaaagca 80700 atctcttcaa tcaatttcct tagatgttat tgtagatgga gtagcttatg tatattttag 80760 acgtagagga aagcaagcag tttttcaaaa attgccaaca tcctattgca gaactagaag 80820 tgttctaaat ggattcccta cagtagaatt taatttagat tattttgaac aatttggaac 80880 agaagaagaa aaagctatta aattaaataa cttcccacca gaaattgtac gtgaatataa 80940 tatttggaaa aatgataatc atcgaggaag aaacagttct aagagacgtc aaaatcaaag 81000 tttaaccaat tctggtacat ggatcttatt aaacccagat acttgtacag ctttttattt 81060 tagcccttca ttacaacctg tactagctaa tagttttttt gctattttag atgtaatgga 81120 actaaaagga attgaaaaga aaaaagctga aaatgaacta tataacttag tagttcaaaa 81180 atttggtttc ttggatgatg gagaacctat cttagaatta cctgaaatgc aagctttcca 81240 cgaaagtgcc aaaaaaatct ttgaaaacag taaccaaaca gacttactta ctactcttgg 81300 agaaatccaa aatgttaact taaatgaggc tgctgctgat ccgattgatt ttgagccttg 81360 gactaaatct atctatgcag aacttggtgt ttcaccacaa ttattctcta cagagggtaa 81420 tatggcttta gaaaaatctg tgaatattga tgaagctatg atgtttactc ttgttgaaaa 81480 atatcaaaat tggttaaatt tcctattaga taatgagttc ttagaagatg atgatgatat 81540 gtttgataca tcactttggt tcccaccaat tacaattaat aatcgtaatg aattatctac 81600 aaaatataaa gatatggcaa ctttaggata ttcaaaatta ttaccagctc tttcactagg 81660 tcaatcacaa ctagatatta tggcttcacc tatctttgaa aattctattt taaacttagg 81720 aagtattatg aaaccattac aatcatcaca cacagcatca ggaaaatcta attcttcagg 81780 ttcaagcagc ggtggtagac caccattgcc agattcacaa aaatctgaga aaacaattca 81840 aaatgctgga ggataaggag gaaataaaaa ttgaaagatg aaagactatt attttttaca 81900 gatattcaat tcttagaacc tgtcgcaatt gaagaaaatc caatgctttc taaggttaaa 81960 attaagattg cacagtcagg gattaataga aatggatatg atattcctaa agcagtatta 82020 gaagatgctg caaaaacaag cttaggtctg acaccaattg ttgcttatta caatcaatac 82080 aaaggagatt ttggtgagca tggaacccaa gcagtatata atcaattagg agaatatgta 82140 actacagccg atacacaagc agtaggtgta attccagaaa atcctattat ttattgggac 82200 gaagacaatt atttagtaac ctatggctat ctatggacaa gtcgatatgc agaattaatt 82260 gatgcattag atgggcgtcc tcaatcaatg gaattgagtt ttgaaaatac aattatgcac 82320 caaaagggca gaattatgga aattactaag actgcttttg ttggtttatg tatacttgga 82380 aatgatgtta cgcctgcttt cgctgatgcc tctattgaag gagttaattt ctcttatcaa 82440 ccattgacag aacaggaaga taaggtagaa gaaggagtag atgaacttat ggatgcatta 82500 aaatttgccc tagataacaa ctttgatgat tctgctcttg gtactcctct tgtagttgat 82560 acagatggag aagagagaga caaggttaat cgtgaaaaga ttactgaagc tgttgattta 82620 ttagatgaag cggctgattt agttgaggat gaagaggcta gaaatagaat tgatgatgct 82680 atttcaggtt tagttgacgc agaaattgat atgaaacgtg aagctgatat aatccctgta 82740 acagaggcag ctcgtcgtgg attacaaggt caaccaggct ataaagatgg tatcgttagt 82800 gtagaaaacc tcaattataa aaaagcaagt aattctttat tgaaaaaaaa tgaagacgaa 82860 aaagaggagg aaaaggaatt ggatatcaaa aagaaaaaag aagaggaaat caaaaaagat 82920 ccagttactc aagaacctgt agaaaaggaa acaaaagtag aaactgaagt taatcccgaa 82980 gacaaaactg ctgaaactaa acaggtagaa gaaacaaaag aacctgtgag tcaagaagaa 83040 gaccaaccca gtgaagaagt tcaacctgga ggacagcctg gagcagagca gacagttgct 83100 aaaccagaga agacagcagt tgaaccagaa gcaacgggag ttcaaacaga tacagttgag 83160 gaagaagcag gacagcctgg agaggaagta caagaagacc ctcaaggagc aaccgctgct 83220 acaattgcag aagaacgtga aaccgctaaa gataaacgta cgagcgcttt attatctgat 83280 gttggcgata atgaattatt tgattatctt attgaacgtg tagctgccgc agatgaaatg 83340 cgtactaaac tacaagaaat gttgggaaca gctgtaccag aaggtttacc agaaggtgga 83400 gatgtaacag tcgaaggaga aacaactgaa gaagatttag gtactgaaac agtaagtact 83460 cctgcagatg ctacagaacc agaaattgat gttaaatctg acaaacctga ttcagaacta 83520 gctgctgaag gtgaagatat gactgatgaa gaaactcttc aagaagatgg taaagaagtt 83580 gttaaagccg ataaaactat tgaagttacc gatggtggag caactgacga aggtggggaa 83640 acttctccag caccagaaca ggcagaagaa aaatctgagg aatctgcacc agaagataaa 83700 actgtagttg aagaatcaga agaagaaaag aaaaagaaaa aattagatta tagtttagat 83760 tttaaagcta ttattgcaga aaatcaacaa ttaacagttg aaaacgaaaa tcttcgtcaa 83820 caaaacgaag cattgctaca atttaaacta aaagctgaac gtgaagcaaa agaaagctta 83880 ttattagaat ttagtctttc cgatgaagga aaagataaaa ttcgtgctaa atttgatgag 83940 ttaactttag aagaggttga agctcaagca gcattagcac aacaccgaga atttaaagaa 84000 gctcatggtg gtcaaaaaga gtctggtgaa ggtattcaat tttcacttga atcagaagaa 84060 actgaactag atgcgttaga tgaagaagat agtttagcgc ttttcttaga aaaagtttct 84120 aagaaaaata aacgttcacg tttttaattt aaaataggaa attatttcta tcaaaatatt 84180 gcaatcaagg tttacttatt ttcatttgaa aatgagtaaa ccacgcaata tttcaaaaat 84240 aaggaggaaa tagaaaatgg cacaacaatt attaaaaaaa gtcggctacg ggcaagttga 84300 gaaaaaccgt attcaaggta tccgagctgg tcgagtttta gcagacctac ctgttaaacc 84360 agaggttgtt aaatctagcg gagatcgtat tgaaaatggt atgttcttag atgcagttta 84420 tggcacaggt tttgaaggaa atttaaaaac tggtcaatta gaattgccaa cagcacaaag 84480 taaaaatgtt ggtttggtat atagtgaggt taaattatat agtgaatata cttcaaataa 84540 agattttgct ttatttacag taaacccttc tattaaccaa atgcgtcaag caccaattta 84600 tgacaaaaaa gaagctccaa aagctacagt tatccctcgt ttgattttcc caacgcctgg 84660 tgatatcttt actactaatt taatcgaaac ttctgatggt gaacttccag aagtaggaac 84720 aacattaaaa ttaaatgata aaggtatttt atctacagca ggaactttaa acgttgttat 84780 tgcaaaagta gttgaaaaaa caacaatggc tgatggacaa gttgcagcaa aacttgctat 84840 cgttgaagta aatccagcag ctacaggcgc gtaattagat aggaggaaat ggaaaaatga 84900 atttaaaacc tgaacaaatt agaactttaa tgttccaagc agcagacatt caaactggag 84960 aatctattga attctcagat aaatcatatg attctcatac attgaatgaa gctcttcgtg 85020 aagaaatgaa tagtttaatg ggaaatggtg gacatcgttt cacagctaac cgtgagttag 85080 tttatgcttt attatcagaa gtcgttgatg atttactacc taaacgagta tttgattcaa 85140 tgaatcgttt cgcagaaatt aaaaatgttg cagatggaga taaagtaaca tttaaacgtc 85200 gtaaaggtaa catccgtgga cgtaactttg tgacacaagt tgctcatgct ggtctatacg 85260 aagtattccg tctagacaga gaagtattcg atatgccaac aacagcttat ggtgctgctg 85320 taggattgga cttagaagaa ttcttagaag gtcgtattga ctttgcagaa ttaatccaat 85380 gcattactga aggctacgaa gaaattcttt ataaagaaat tctacgccat atggtagcgt 85440 taaactctaa cacaatttta cctgctaaca acatctatag tgtagctggt tggagcccac 85500 gtcgttttgc agcattactt ggtatttcaa gtgcttatgc aacaccaaca atctttactt 85560 catatgtatt tgctgctgaa atgatccctg aaggacattt agcaacagaa aaaatgaaag 85620 aagaatataa ccgtaatggt ttcatcggaa actataaagg tgctaacatt gttgtactac 85680 cacacagctt ctacaacgaa acaaacgaag cagaagcagt aacattacca ttaggtatgg 85740 cttggatcct tccagatgta gacaacaaac cagttaagat tgcttttgaa ggcggaatgc 85800 aaactaaaga agtagattta gacgactggt ctaaagaaat gcacttctac aaaaaaatgg 85860 gtactatgtt gatggctaac ccagctatct gtatctatga aaacactgcc cttaatgaat 85920 ggcctgtagt taacggacca attattaaag aaacagaagg caatgttaca ccaacatata 85980 cacgtttggt ttaatataaa aatccgaaga ggttcgagtt taagactcgg gccttttctt 86040 ttgctctttt ggggcatatt tgtttaagta tactttagtt agttctaaat agattagaac 86100 agagaaaaag gagagaaata aatatgaata attcatacct aactattgat tttagttatc 86160 ttgtcgaggg gttaaaagga gatacaatga ttcctgtctc aaatatgggg cgaaaaagag 86220 ttgcttatat cattgaagaa attggagtaa caagagattt tcgtcaattt caagaaggtc 86280 agcgaccaga tactaaaaat attccatttg gagaacttta tgctttaagt aatatgcctg 86340 gaggcatgca attaatttgg gacaacctaa aaattgatag caatgacgct cgtcaagctt 86400 taggattacc attagcagaa gatactccag aagtagaata tgaccgagaa actgtagcta 86460 acattgtaca aaaaggtaca gaagatgaaa tcttagacat gctagagttt ggtccttatt 86520 atattgcaga atggattaaa gaagaagcta ttaatgtaga tagttctaaa cgtcgtcatt 86580 ttattggaca agttctacaa attaatattg atgctcttga agagaacgtt aaatgggctg 86640 ctggggacga agatgctgga cgactacaat accaaacaat taaaggtgtt aaaactaata 86700 ctgctgcaag aagcgcagga cgtcgttcaa ctggaaaacg agcagaaggt aaaacaaaaa 86760 ctggaacagc aagctcaggg cgtaagcgca gaacataaaa ataagataaa atagaatggc 86820 tggcttacga gccagtcatt ttttttagga ggaaaaggaa atggagaatc aacaagttgg 86880 aacaagtttt tttgaaattt attgtcgttt tttagataaa atcactgatg atatgtattt 86940 agaattaagt cttgaagaca ctctaaaaat tattgaatct atttttatgg attccttacc 87000 agaatatagt tatccaagat ttagaattgg attatacgat ccagatattg ttacctctga 87060 tgctttagat gaagaaggga atccagttgt aacaggagcc ttcgttgata ctttaaccaa 87120 tgaagaaaaa gatattattg ctgaaattat gttattaaat tggttaagac gtcaattaaa 87180 tacaactcgt attattcaaa tgagatattc tacatctgat ttcaaacaaa cttcacaagc 87240 agcacatatg caaagattaa atgcagttat tttagaccaa caaaaacgta ttaaacataa 87300 attgaattta tactctaaac gtaaaattga taaagatggt tatattgttc ctaatgcgga 87360 taatctttct ggagttggtc cacgtgctcg ggatattatt atcacacagt taggagttgt 87420 aaaacgtcat gactaataca gaatttaaaa atggctttgg tattaatgaa gtaccatggg 87480 gaatatatat taaacaaaag aaaaatcaag tatttaaatt actacctctt aaagaagaaa 87540 atggcgaatg ggagaaacag ttacaaacta tcctacttga aatgggagga ataattaatt 87600 tatctcccca agaggaagta gttgctataa caattatggc taaattagct gggctcgaaa 87660 atgaaaatga ttttatgcta tatagaaaaa cagtatttga aattatctcg ctcttggagg 87720 actggaaaaa agaaaggagg tagttaataa atgaagatca ctgatattgg caaagcaaac 87780 aaagtaaaag ataatcatga ccatcgaata gagagtggct atcaagttaa acctagtgat 87840 cctgtagata caaatactgc ttgggactgt ttaaataatg caagaaaacg tatggatagt 87900 agtttaaagt taaatatgct agacaaaagt tttcaacaag cgttagattc agcagaagga 87960 acagaattgt ttacagtatg ggaaaaaggc gacactgacg tttggaaggt tatacttaaa 88020 aaccaaaaag ataattggca acaagatgtt aaatatatta atgctttgaa tggagtcggt 88080 tttgaagtag gtaatacagt aacttggcaa agacttggga ttagatggtt aattacatgg 88140 caagattata atataaatga atttttcaga ggagaaatcc aaaaagctga acatgtggtt 88200 agatggaaaa atcaacatgg acaaattcaa caacagtggg ctgtagttca aggaccgatt 88260 gaaactcgtg ctaaatatga acaaactaga ggaaatacta ttgtaggtcg tcaaaatgat 88320 actgtagaaa tatggatggg atctaatagt cctaaagacg ttaatgattt aacaagatat 88380 gataaaataa atattaaagg aagatgttgg agaattcaag taattgatga tatttctaat 88440 cctaatattt tacgtttttc atgcgttgaa gatttcaaca atccggtaac agatgatatg 88500 gtagctttaa ttcctaatgg taaaattgat tttgccccta acgaagaaga acctaaagaa 88560 aataaaattc gtattgttgc acctgctaag attaaagaaa aattagttag cagagtttat 88620 gcaattaatg aagagacgtc tgaaaaagtt aaaggaacat gggaaatttc tggcgcaacc 88680 tttaataagg tagatgaata cgaggtcgat gtaaaaggaa cgaaaattgg gaacataatt 88740 actataatgt ttacagacga agaaggaaat tctaatatag taacagctaa aacagtatct 88800 atgttttctt gataaaataa tgagttaaag gagagaattt taatatgcct atttatgata 88860 gtaaaaccaa aaaagctaca aaagatatgt atataaaaga tagatttatc acaagcggcg 88920 acaacctaaa tattattgct aatcgccttt tggatagcga cagattaatt aaattgctta 88980 ctcgcaatgg ttcagatgtt ttaacagatg acagacctgt tacagacgaa gaacgagctg 89040 aagcaatgag aactaatatt ggtgttatac caattataga taaagatatt gaagtaaata 89100 cttttattgc agtacaaatt actgatattg tgcctacaat acaaggatta acatataatt 89160 ttgtgtttga tattttgtgt aacatagaag tttggaattt agatggatat aaccaacgcc 89220 catatttaat aatgaatgaa ttagaccaac ttttttcaaa tactaagatg aaatctttag 89280 ggccagcaac attcttagga gcaactagct tgaaaattaa tgaaaaaatg cttggttata 89340 ctatgatgtt ttcattttcg gaaatacaat agtaatggat attttaacaa aagttgctgg 89400 cacagacttt tattttatac cttttgctac attaattcat caaccaacaa taaaagaaat 89460 agctttatta ggagaaaaaa ccctatttga tgccttagca atctttgcgg gttcttctcc 89520 agactatttt aaaaaacaag tacttaaagg aatgcccgaa gaagaacgtg gagcaacaga 89580 agttgaatta aattatacga taacatcaga gctagatgtt ttttataaat attgtttggg 89640 aactcaagaa aaaagagtaa ttgaatcctt tttattttta atttttgaat cattacgagg 89700 agtaaaattt attcccatag gagaaacaaa aatgttgctt caactatctt ttgtagatat 89760 tcttagcaaa gaagaaaata aaataaatac tattactctt gatgcagaaa atttctcaga 89820 attaaaagaa actatttctg atatgtttac ttatcaatca gaagaagatc aagaatcgaa 89880 actaaatcct gctggagaaa tggctcaaaa aattgctgat aaaatggctg cagcagcaga 89940 aagaagagct agaatttatg ggaaaagtaa ggctaaaaaa gacgaagagt ctgtaatagg 90000 aacaatggct tcaattttag ctacgagtga tggtatacca ataacagagg tcttaaaatt 90060 aacatttccg caggttctta ttcagttaaa tcgaacacaa atgttccaac aatatcgtac 90120 acagattaca cttggggctt ttggaggttt agaagcagat gatgtagtaa actggcaaca 90180 gtcagtttaa ttttactatc aaaactttat aatacaagct tggttttttc tagatgtatt 90240 agggaatata attccaagaa caactcatat aagaggagga aaaacaaatg aatgaaaaat 90300 ttggcgttcg agaaatagta gacgtagtat ttaaggctaa aagtgctatg actgttggta 90360 acactaagta taaaaaaggt gaaccagtta tctactttga ctcagctaaa acttcaactt 90420 tggagtctac aactgctact gtttacgctc aaggtggacg tggtaatgct cgtttacttg 90480 cttgggaagg tgacaaaaca gttactttca actttgaaga agctctatta tcaacacgta 90540 gttttgcttt gttatcaggt ggtaatttaa cagaaaatac tcgtgtaaat atgcacgtta 90600 cagaacgtgt agaagttaca aaagcatcta tccaattggg agaaaaaact gctgctgaag 90660 taccagtact tgatttagct ccatatctac ctaaaaatgg acaaatccta agtgctgcag 90720 accctacttt ggtaacagaa ggtggaaatc ctaaataccc tgaagctggt atttatgtaa 90780 tggaacttaa cgaacatggt gaaattactc gtcgttttga tgttacagca ggcgctttac 90840 aaacagctcc ttctacacca gctctatcta ctttggctga gtcacaacaa ggtgacgata 90900 ctgaattaaa tgtaactaaa aaattctatt tagcaagtgc ttatgctgga gatagtgcaa 90960 ctacagctgt ttcaggcgca aatgctttag atgagtctaa agtttactta gttgacttct 91020 atgtagctca accaggttca aacttgacaa tcacaccagg taaatttgca ggaagcttct 91080 taatcgaagc aacaacattg ttccgtcgtc aagctgatgg acgtgacttc ccagctcaat 91140 ttactattcc aaatggtaaa atcagttcta acttcacatt cacaatggca tctactggtg 91200 atccaagtac attcccattc caagttgaag cacaacctga ttatcttcca tttaacaaaa 91260 aatgtaaagc attatttgca ttggatgttg ccgaagaacc aattggcgaa gatgtgtgct 91320 aataattata aaagacctag tatttttact aggtcttttt tttgtaagta attcatattt 91380 tttattattg ataaagacat acgagaaaaa ggagagaaat atactatgag taaaatatct 91440 tttagtaaac ttaaattaaa ggttgatgat agtgtcaaca ctgtagagat tgaaggtgca 91500 gatggtacaa tttatgagat tgaagtaaaa caatatcttc caattgaaaa caagattaaa 91560 cttgtttctt atgtaattgc gaaatctgcg aatggtggcg ttattcgaga agatatgcta 91620 gatgcctatc ttggtgtaga attagttaaa gaatatacta atttttcatt tacagaaaaa 91680 atgctccaga atgatgcaga tttatttgat gtactggaat ctaatgatat tgtgggtaaa 91740 gtacaagatg caatgaaccc tgcagaatta gaagacatta ttcgttatat taattcttat 91800 tcagaccaaa tgcaaaaatc tattcaatct tctgtatcag gatatactgc tcaagagaaa 91860 gctattgaag gcatggtagc taaatttatg agtgaaattg tcagcaacga atcagaaaca 91920 gtgtcagata actaacatta caccttctta tcacaaggag gaatgagtac gaatgtcttt 91980 atcagatgtt ttaaatgcgg ctcctggttt gtcagattta acgattggaa tagttgctat 92040 ccttttggta gtctggttag ttgggaaatt tttagataaa caacaagaac aaactatgtt 92100 acacaacgaa caaatggaca aaacgttcaa aattatagca agttttaccg aaacattatc 92160 tgaactagtc gcacttatcc gagaaggaaa tgaaaagaca gacaacgttt ccgataaagt 92220 tgatgaaata aatcgtaagc tagaacactt tgaaattcat gacgaaaaag agcatagcga 92280 gaaaaaacaa ggagacagga acgatagtta atctatctcc tgtctctatt tttatcccaa 92340 aaggagggaa taggatggcc gataatctgc caaagttacc ttatacgtta caaaacatac 92400 gagacttaaa atttaattct gtctataatt tacagccttt acaatcatac tctttaagag 92460 attttaattc aggcgataca aagcatagaa tatatcaaaa ttatattaat gcttatgaag 92520 cagattttac ttctagggca ggaatgaatg ccgaagaatt ttctaaaaaa gctggtcttc 92580 atagtaaatt agttacagaa aatacagatt ttattaaaaa ttttaaaata gcacaaaata 92640 atttcagtat aaataataca gagttaaaca aatatgttaa tgatttatta tctggagatt 92700 cagatagact acaaaaatta gctggcaaag aaaaagaaat tattaaagta ttagaaaatt 92760 gggctaactc agttcaagaa tatcttgatg tactttcggg ggtagatgat ttatattatt 92820 ttagaaatat ggatcaagct cagataaatg gttctatttc tatggcaagt ggaagaaata 92880 tttctcaagc ctttagatta gaacgaggtg ctatgacagc gatgcaaaat gcagacgctt 92940 tgatagtagg tttaaaacaa tctattgata aaatgaatca agaaggatta tctgttaaac 93000 aattatcaaa tatggttatt acaactccta gttatgaagg agctaaagga caagcaccat 93060 caacaataaa aacattggga aatttaaaga caatggttgg gtcattgagt aatttaatgg 93120 gttttgcctt cgaagcacaa ttagcaagtg gtttggaacg ccaagtcaat gaagcactat 93180 ctgacattgc cctcttggga ggagcagaag gagtttcggt aaccttgagt gatggacgac 93240 cttttaaagt taaacaaagt aaaacagacc ttagagcaac tgttgaaggg attgacttag 93300 gtttttcttt aaagaattta tctaaaaatg gtaaaactaa acgagtttca ttaaatactt 93360 ctaatacaaa tcagcttatt gctttaataa gtaataatca agatttccga tcacagttag 93420 ctggaacact aatgtggcgt tctaattcta taaaacgaac tcgtgaccaa ttaacaatgt 93480 ttttagcttc cttgtctgca gactatgcaa ttgcaatggg aggaaatgat cgtattgatt 93540 atatggtttt taatgacaaa gttattagct taggagagta ttattcaatt atggagaaaa 93600 gattatctct taaaattcat cctagtgcta gtcagagttc ttatcaaaat caacttagag 93660 agttgagaga acgggaaata ttagctaata tgcctgttaa atttacaaca tagagagcaa 93720 aaggagagga attaaatggc aaataatgta gacgttggtt taaagtttaa aactacctac 93780 gaaggtcttg atgccggtaa acaggaccta aaagagctaa aaaacttaat ggactccatt 93840 ggtaaagatg gagttaaact taacttaaaa gatggagaaa ttgaacaagc taaattacaa 93900 atttcagctt tggagcgctc aattagagaa gctgaacaaa gtggtggaga ttttggtaga 93960 attttccagt ctaatttaaa tgcggtacaa aatgaagcta aacaaactgc aactagtgtt 94020 tcaaaggtag agaaagaaat taaatcttta aaccaaacat ctaataatgg tggaggattt 94080 aaatctatct ctaacgctgc tcgtctagca acaaaagaaa ttcaaggaac aacaggtcaa 94140 attgatcagt tacgaatgaa tttacaacaa ggtatgggac aaactattgc ttttggtgct 94200 attggagctg tgacaggcgc agtaaccgat gctttaaatg taacaaaaca attagatgaa 94260 atttcaacag atattagtat cgtttctggt aaaactagaa aagaaatgga aggatatcgt 94320 gatgccgctg tagatgctgc tgatgcctta ggaaccacta caaaagatta tttagatgct 94380 tctttaatct ttgaacaaca aggtggtcaa gcagcttatt acgcaaaaga attagcagaa 94440 gctactgtaa cagccgctaa tatttctaac gtagcaacgg atcaaatgtc tgaatatctt 94500 acagctacaa ttaatggttt ccaactatta aaagaaaaag gtggagaagc tggtacttat 94560 attactgatg taatggctaa attaggagca gcatctggtt ccgacttagc tgaaattgct 94620 acaggtttaa cacgtactgc taatacagca aaagacgttg gttttgaatt tgaagaaata 94680 gcaacaatga ttgctactgt ttctgaagta actcgtcgta cgccagaaac aatcggtaat 94740 gcatttaaat caatgcttac ttcgttcacc caattacgtg aagctggaga agaagaagta 94800 gaagctttca caaataaagt tcaagaagct tttaaacttg gtggaattga agatatttct 94860 ttatttgata atgggaatct aagagatgct tcagatatct ttaaagatat tgctgctcgt 94920 tggcaaacaa tgaataaaga acaacaatct ttagtttctg aagctgttgc tggtaagtat 94980 caagcagaaa ctttccgtgc gtttatgaac aaccaagagc gatacaatca attacttggg 95040 gaagcttatg attctgctgg aacatcggcg caacaacaat tagtatatat ggactcttta 95100 aaagctaaag gagaacaact taaaaatgct tggcagggcg ctgtaaatac agttgttgac 95160 tcagatatgt ttaagggtgt tttggaagac gctactaatt tcttaaaaat tattggtgac 95220 gcaaaaagtg gacttggtgg tttagctaca gcaattgctc ctattgttgg tattggcgga 95280 caaatgtttg gcgctaatca ggttggagaa ctttattcca ataatagact aaataaacaa 95340 gccaaagaaa ttacagcaag tttagagaaa atgaaaattg cacaagaagg taatttgact 95400 gcgttacaag aagaatacaa atctgcagca gaagtgaacc agattatgtc tactcttggt 95460 ccagaggctg cgagaaacta tgaagaagta actaaagaaa ttactaaact tgatgaaaaa 95520 ttaaaagata ttccgaaaaa tgctgaagag gtaaagaaaa aagctgaaga agctgcacaa 95580 gcgattaaat taaatggtta ttataagggt gtagacgaaa aaaatgttac tagagcagca 95640 aaaggattag aaaatgaagg taaagacgct cgaattttag aaaatattac caaattaaat 95700 aaagctagag aagagttaca aagagcagaa caagtatcac ttaataatct ttctacttta 95760 gaaggagcag ttagtttact tgattctaaa gcttctggag ttagaaaaga actgcaagaa 95820 gaattagttt tatgggaaga tgatattcga agcgctttat atgaaaatga aaagttatct 95880 aaacaattta ctggagacct tgataaaatt cgacaaggaa tagatagcca atatgatagt 95940 catgccagcc ttttaaagat tcgtaaggaa gctaaagtta tagaaaatca aattcgagat 96000 gcaatgatgg ctgaagaata taatgccagg gaaaatgtta ctaaagctga aaaagaatta 96060 gaaagcaaag ctaaaaaagt agtattagaa gaacaatata ctaatgaaat gaaagaacaa 96120 aataaaatta ttgctgatag aaaagtatta gaagacaaag ctcaaagtac tgctcaaatt 96180 gcaaaacgaa cagaattaat tcaaaaaggt gttagaggaa tgagtttggc ttatagcaca 96240 atagttccta ttatagctac ctataattct gttcaagaaa aaagtattag caaacaagat 96300 ggtgtaattg caggtttaca aactacttct gcaatgttaa tggcatctat gaatccgtgg 96360 ggtatggctg caggggccgc tgctggagca ttttccttaa tagttgataa atttgactta 96420 ttcaaatctc gtggagaaaa agcaaaagaa gttaatgaac aattgactaa aacttttatg 96480 tctcttcaag aaacagttaa tgggaatatt tctaacttaa atgatattgc agatacctat 96540 aaacgttttg agggtgttaa tgctgaagca tttattaata atgcaccaga cccaagtgat 96600 acagaagctt ttgaaaagta caataaaaat ctagaagatt atgataattt agctggtaaa 96660 attgctcaaa caaatccaga attagtcaaa ggttacgatg atactggtag agcaattata 96720 gatctatcta aagattttga aactttgatg gagcaacaaa aaactgctag aacagataat 96780 tatcaaatgc tttccgcaaa tagaaattca tttattgtac aacaagtttc tgacttacaa 96840 aatgctaaat ctagtatgaa tgattattct gttgaagttg ctaaattaac caaagaactt 96900 gaaaatgcaa aaaaacaggg caaaggtgaa aaagtagatg atattctaaa agatttaact 96960 aatgctcaaa ataaaatttc agaagtacaa aaaaccttca gtgaaactaa agctaatatt 97020 caaagtagta ttgtagtacc ttttaatgaa gcaaacgatg aatttaataa attagttgac 97080 aaagctcctg aattaaaaga agcatttgaa actttttctg atacttatat gaactctaat 97140 ttcctatcag gaatggcatc tgagaatgac gaagaaggaa tgaaaaatct tgttcaaaat 97200 atgggtctta ttaaagatga gtttactgaa atagctcaac aagaccctaa aaaagctcaa 97260 gaattcttag ataaaattgc caattctagt gaatgggcta aattagcttt atatgatgtt 97320 caaggaaata ttgaaacttt acaagaaaaa atgtcggaag ccacagatcc aattacagat 97380 gaagtacagt ctcaagcatt aaaatttgta gataaaataa ttccagagga agatgttgaa 97440 gaagcccaaa aacgtattgg agagcttgct tcagatctcg aagaaaaagt tgaagacgcc 97500 aataaaatga aatttgattt ttctaaagca gaaggcgctg actttactga ggatagaaaa 97560 gaaatcgaaa atattaatga taaaattaaa gattacgatg aacttcaaaa tacaatgtat 97620 gatgtttctg aaaaatacgc tagaatgtct aggactgtta aaggttttaa tgaaagtcta 97680 agtgatgttc aagatttaaa tactgcaatg gaagcattaa aaaatcttca aaaaaatggt 97740 tttgacgaaa gccaattaga aatattaaaa caaaaattcc ctgaactaga aaaaactatg 97800 agagtttctg ctgagaaagg tactgaggct tttcaaaaaa atgcttcaga tttatatacc 97860 ttcctatcag aaaggcaaca agccgctata actggtatgg tcatgaataa tgagcagttt 97920 tatggagtat ggatgcaaaa taattctaag acaattaata aaattgctga agattatggc 97980 attgatgcat ctaactacca aacgttggct gaatataaaa atgctctaca atcactaagt 98040 cttgaaaacc tacaagttat tgcaggagaa gaagtaaaag agaatgataa aaaaaataaa 98100 gaaattttag ctggggaagc agttcatgta agtaatatga tttctttaac cgacatctgg 98160 tctaattcaa atcttactta ctcccaaaaa cttgtagctt catttttaaa tgcttatgac 98220 gatatggcta atggactagg aaacgcctta agttatatgg aacagcttct aaaaggatgg 98280 ttaaatacag ctcataaagc attaggtgat ttttttggag gtatggtaaa aaaacttcca 98340 aaaggaatga gggacgctct tggtcttgat gagatagaca aatggggcga aggtggagtt 98400 tctgaaagct tctttaaaaa attagatcct tccactttag caaaagatta tgtagataaa 98460 aagaataaag aagctcaaga tgaacaagag aaaaaagatt ctttgattca acaaattctt 98520 gaaggtaaat tcaagaataa gaacggtctc tcaaatgaag acttgatgaa aaagttagat 98580 cttaataaac cacagtcatt agaaggtctg aatggtgttc ctagagaccc agaaaataaa 98640 gatgttgaaa aagatggcaa aattgaaaag gaagacaaag ataaggataa aaaagatgtt 98700 gataatctag aattagaatt agatagatat tacaaattag atcacatctt aagtgaaatt 98760 gaaaaacgtt attctgaatt atcagatgct aaagatgctg cttatggtaa tgatagatta 98820 aatatcatga agcaagaaga gcaattatta gctcaaaaag caggagtatt aaaacaatat 98880 acggctgaat taaataaaga gcaagacgaa ttacgaaata aacttgcctc tcaaggattt 98940 tctttttcag caaatggaga tattgataat ctaaatcaaa ggttaactga tttgcaaaac 99000 gctgcaaatt ctaaaaccgg agacgctaaa gaagatgcaa ttgacgctgt taaaaagatt 99060 caagaagagg cttcacgtta ccaagatatt acatttaatt taattccaga taaacagaaa 99120 acattaaatg aaattaaaaa acaactttct gaaattgcta gagaaagaat tgaatatact 99180 gttaaactta gagttgacaa agatgaattc aaatctgatg ttctagatgt tgttaaagaa 99240 atgcaagatt cttatgcaga ccttgatgaa aaagctcaaa ttgtcggcaa agaaatgaaa 99300 caagctttag ataatgtttc ttattggcaa tctatgatga atcaagttag aaacgaccgt 99360 ggtttaacag attctgacag acaagattta ttacaagaat acaataaaaa cttgctcgat 99420 gcagtttcaa aagcaagaag tggttataaa gaattattag aagtacaagc tgattttgtt 99480 aaacaatcag ttgaagctat taataaggta aatgatagat atgataattt atttgaaaaa 99540 gctggacaac tagcagctaa aacagaagag ttatatggta caaaagctta tggtaaaatt 99600 gttggttatt tcgataccca agagaaggcg attaatggtc aggttaaaaa tcttcaggaa 99660 gctcaacgta gaatgattaa atatcgaaat tctgttaaac aagggtcaga tgcttggaat 99720 gaggcaaatg atgctattaa cgaaatggct gattcaatta gcgatgcttt attgaaaaaa 99780 cttgagttag ctcaaaaacg ttttgaatta ttcactcaat cagttgagaa tggattccaa 99840 aaaatgtttg gtgcttgggg attagatgga gtcattgatg attttgataa acttactgaa 99900 aaacaagata aatatttcag caactttgaa aaatttacaa atatcagtag taaaatcaaa 99960 gaaattaacg acgaaattgc taaaacacaa gaccctaaac gcgccaaaga attagcagac 100020 tttagagata aagaattatc cgctctcttg gctcaagata aagtttcaca agatgattat 100080 gatcgtgcaa tgaaaatgta tgatattaaa caaaaagaat tagctttaca agaacgagaa 100140 aatgcaaaac gtacagctca attagttcgt gatgctaatg gtaacatgac ttatgaatat 100200 gttagaactg aaacagaaga tttatcaagt gaaatcgcag acttacagaa acaaaaagat 100260 gaattatatg cttttgattc tgataaagta aaagaatctg ctaaaggtgt atttgatact 100320 atttctaaat accaagataa aataaaagaa ttacaagata aaggattaga tcctgcagaa 100380 tataagaaag agttacaaaa acttcttgat gaagcagaag atgaaattaa agataaaact 100440 aatgaaatga ataaatggtt agaaaatgct ggaaaagatg gattaagtaa tctacaaggt 100500 atggttgcaa atggtacgat tacaccagat caactaggag tagataaaaa tacgttatca 100560 agtattttct cagcaatgaa agatggtact ttaacagtac aagatatgtt aagcggagac 100620 tttgatgatt ttgcaagttc tattggtatg acagccgatg aacttagtgg tacaatggat 100680 aaattattag aattaatctt aggagataac atggatattg cagaagcaat gatggacgca 100740 agcaataaat ggacttctac tgctcaacaa aatgtaaata acttaggaaa tgcttatgct 100800 gagtatatgt ctcaagctac aaaagtttta aatgattata gcagctctac aggaagatta 100860 aatgatttgt taaaccaaac aaatcaagct tctcaacaag ttatttcaac tattgataaa 100920 caaactcagg caatgctaag aagtcaaaga caaactgatg caacatcaaa tagtgtttta 100980 aatcttcaaa atagattaat tggttcaaat ggaaattcag gactgtttgg ttcaatggtt 101040 caattgcaac gtactatgaa tgagcaatta caaccttcaa tgataaacac tggcaaacag 101100 accgatatat taagtgggaa aacttataat tcagcagtaa aatataatgc aatgggaaac 101160 gaagctaaat tcgcttacca acgagttaac gcatttagtg accaacctac aaaagtagcg 101220 atgtcaaata ttgatagaat tactggtaaa accaataata ctgctggagc atttagaaat 101280 gttaaacgtg atgcaaatga aggtagaaat agtattattg acttcagttc agcaattagt 101340 actttagctg gatatggttc ttataaagtt tctgagggct ctaagaagaa agctgcttca 101400 ctagatagtg gaggttatac aggaacatgg agtaattcaa caaataatgc tgagggtaga 101460 atggcaattt tacatgaaaa agaattaatt cttaataaag aagatacaaa aaatatttta 101520 gaatcagtta aaatgcaaag aggactaatg gaatctatta agggcagagg ctcttctgta 101580 aaaaatagta ttcaaaatat gagttctaat atttctaata ttcaaaatag taacagaatg 101640 agtacagttg aacaaaacat tactctttca ccaaacttcc ctaatgctag agatcgaagt 101700 gaaattgaag cagcgttcga aggcttatta ggcaaagcat caacttatgt tggaaagaaa 101760 tagaatataa aaactagggg cggaaatacg actattttcg ccctttcttt ttaatctcaa 101820 taggaaaaaa gagaaaaagg agctgacatg atatggataa aagccaagag ctttttgatg 101880 cgattcgatt aattgcaagg aacgaaatag aaaaacaatc atccacagat actgtgattg 101940 gtacagtaat tgaacgtgtt gaatcaagcg acgcttataa agtttcttat caaaatattg 102000 aaattctagc aagttctatg ggtggacgat ataatgctgg agattctgta tatgtaatgc 102060 ttcctaaagg aagattagat ggggtcaaat ttattattgg taaaaccaat gataggactc 102120 cgactataac aacaaatgca aatggactat ccgattctac tcttggtatg attcaggata 102180 ttattaacaa ttttaacgat ttaatttcag ataataaagt tactccagta gaaaaacaat 102240 ctttacaagt tcaatgggaa caaattaaaa agtcttatca agagatttta aataattctg 102300 caccttatcc agaaattgat ttaactggtt taaattctaa atataaaact ttagaatcat 102360 taatgaatgt tattttagga aatatggaaa ctactacaga ggttgatgga aatacagtaa 102420 gacaagtttt atctaattat ttaactgaag atacctcagt aagagtttta atccaagagg 102480 cactaagaaa tgaaattact tataaagctg atattgtttc tacaaatggt gaaagtttta 102540 aaaatggtgt aattaatact attttaaaag taattgctat gagaggtaaa aaaactataa 102600 cttcatcatt tgagccagaa aatattagat ggtataaaat gaaagatgat ggttctattt 102660 tacctaattg gtctaggact ggtaaaagta tccctattac atcagaggat gttgacatta 102720 aacaagtatt tgtggtacaa ttatatgtag aagaagcaat ggtagcacaa gatacaataa 102780 ctatagttga tttaaatgat atagaaaata ttaaattaag tttaactcct aagttaagta 102840 gaactcaaac gtttgaccca accactaaaa atattatacc agattatact atacaaaatc 102900 aagtcattca agccaagact ctaaaaggag cagatgatat tacttcttta gtaacgcatc 102960 aatggtatta taatggagaa ttaattacca atgatggtag gtttgaagtt caaaacaacg 103020 ctttgtttat caaaagaaat ttaatggact cagaaaatcc tacaatgatg attgattgta 103080 gagtatctta ttatagtgaa gaatataatt taacccttga ggattctttg tctttagact 103140 tctcttatgt atctaatgga gaagatggag aggatggaaa gaatgctctt ttagttaacg 103200 ttcttcatcc atatggcaca accatcaaga ataaacaaca atctcttctc ttggtatcac 103260 tacaagcata ttttgctgat aaagatgttc tttctttatt aagcaataga aaatggtttt 103320 atgcagatga atcagttaat tcttctagtc cttattatga taaagatggt ggagtaggct 103380 ggtctctaat tagcgagaca aataatttga atggagcttt aaatggatat gatacaaata 103440 tcctatctat ttctggaaat ggctttaacg gcagcatttc tatcaaggtg gtagcttact 103500 ttgaagaatt taaaggagct gcaacaacta ctttaattga ttctactgat ccaattacag 103560 gagttatctt gggcaatacg caattaaaaa atggtgagcc aacttcatta caaatagaag 103620 cttatttagg acaaatgaaa ataacagata cttctaatta cacttttgtg tggaatttag 103680 aattggtaga taatagttct agaataattg gtccaatgga acctttcact aaaacgacag 103740 tatggcccaa gtatggagaa tctattatat tagaatgggg agatatccct gaaaacgata 103800 atgtttatgt aacatgtaaa atatatcgta atgagtagat aaatatagag ataaaggagg 103860 gaacaagatg tcattaactc aagtaggtac agcttctgct ggtatttcaa aagtaattga 103920 cggacaaagt caatatatat tctggagatt ttcagcaaac ccagatggaa atcctatgac 103980 agaaagacca acagaagata caaaatatat gggggtttat acaagcacaa aaaataaacc 104040 ttccgagaac ccaagtgatt atatctggac taaaattaag ggtgaagatg gttcagatgg 104100 acaaaattct tatagtggtc tcttaactga tgaatttatt gctgttaata cagatgcaga 104160 tgggaataat ggtaatttct ctaatgctag tacgtcaatt attttgtttg aaggaacaag 104220 agaaaataca tcagagtggg atatttctgt tgaagtaact gcaggaattc aagggagttt 104280 aagttcttca aaggacactt ataaagtatc aggaatgact gtagactcag gagaaataac 104340 ttttattgct aggtataaaa caactacttt gacaaaagta gctaagattg caaaagtaaa 104400 acaagggact cctggagaag atggggagag tccatacaac gtagaactta tatcatcaaa 104460 tggtttaatt tttaaaaatg gggtaatttc aacaattatt catgcgatcg tttttaaagg 104520 aaatgaaaat attactgatt tattagacgc gaatcagttt agatggacta aaacaaatgc 104580 tgatggatca ccagatttaa tatggaatgc aatgcatgct ggtggtacaa aaagcataat 104640 aattgaaaca gatgatgtta acaaacgtgc aacatttagc tgtgacatta tcgaagagat 104700 ataaaggaga tggaataata atggcaacaa tttcaagcgg tcaaattaca attattgatt 104760 tatatgatgc tcctgcatta aatgcgtgga tttcagcaag tcaaacaaca actcaaacat 104820 acaacaatac aactaactca tatacaccta attttgggtc ctctgcacag gttttaacac 104880 ttaacttaac aaaagcaggt ggaacaggaa gtttaattgg ttctgctgta tcaggcgtta 104940 aatggaaaaa aacagtaggg tcaacaacta ctgaaataac ttctacaaca aatactgata 105000 gtgaatataa gagcggtaca tcaaacagtg tgttaacaac aaaagtaaac gttccaacaa 105060 cagataatgc tgtacgttgg acagttgaag gagtatatac agacccagat acttcgctac 105120 caattacttt ccaagcgact atcgacatta ctcttgttca actagctaaa gctgcggtag 105180 tagcagtttt gagtacacct aatggaaact acttccgaaa taatacgcct tctagtctga 105240 agttaaccgc tgatgtttat aaggatggaa acttaagcaa tggctctcga aaatataaat 105300 ggttcgcagc agattcaagc gttgtaacat ctcaagattc tgacgctgga gttggctgga 105360 gaaaaattac agcaactaca ggaacatcag gagctgttgc aaattcagga tttgatactg 105420 cagttacagt tcagggggtt ctaacggtct atccagacgc agtaacaaat ggacaaacat 105480 tcctgtgtgt cgtcacagat aatgttggag gaacttctgg aactaaaatg aagcagtatg 105540 taaccttgat ggatatggac gacccaatta tggtagtcat tgagtcctcc ggagggaaca 105600 ttttgaagaa tggcgtaggg tctactactt tgaaagcacg attgtatcaa aatggtgaag 105660 aaatcgattc aggcggcaca ggctatactt ataaatggtc taagtggcag aataatgtaa 105720 tggtaccaaa cttcggcggg acaagtaatg cttataaaac tgggaaatcg ctgtcagttg 105780 gaagtgctga tgtaaataat tcaacaacgt ttaaagtgga ggtagaaagc aaatgacaaa 105840 ctacattgct tatatcattt taaataatga acggacaaaa attgaattta gtacagatag 105900 gaatcctatt gaatatttgt ggactaagta cggcatggat acgtatatcg aatccttcga 105960 ggaaatcaaa gaagagggtg attagatggc tacagtttca cgtggtgaaa tcactataac 106020 aaacgttaat gacggacgta ctccatactt ccacacggct tataccaaca gcactgactt 106080 tggtagttac gactactccg gaaatcctaa cttaatggtt ccattgaaag ctagtgattt 106140 ttatccacaa tctggggcta ctatagaaga ctatagggat gccttaaaag taactttcac 106200 taaaactgat ggggctgttt ttttaggaac tagcacaaat attccagctt tattgccaaa 106260 tactcaatat acattaagcg cagatgttac tgtttctgag ggatacacag gaaaactaga 106320 aaatttaagg ttagggtata ggaaatttcc tggcggaaca attatattac cactgacagc 106380 taaagatgcg gttgtgggtc agaagactag aatatctata acagctaatt ctagcgctgc 106440 aacagactca tcaaagtttg atagaatgta cttcacaatc aatacaacat caacagagcc 106500 atttgtcgga acggctttaa tcgaaaatat taaagttgag caaggaacag ctgccacacc 106560 ttaccaacct aacatcaaag gtgccccatg gtactttagt actgtttctt taggtaagaa 106620 tttgataacg aatgctcagt tcccaattaa aacatcaaat gaccctattg ctgggtttga 106680 tattttagaa gaattaacaa taggcgaaac ttatacggta tctttaaaag ggactaagct 106740 agctgataaa gaatttcaat tatactatgg agacgctggt aactaccaag caactttacg 106800 ccctgtggaa ggtctaacag atgtgtggag tgccactttt acagccagca attctactgg 106860 aacgtcaaat ttacgtaggg tagctttatg gcaaaaacct aattctgtaa caaatggtaa 106920 tgttcaaatt gactggctca agattgaaaa aggagataca agaactccta acatcgataa 106980 ttataactac ctaggaaact atactgactt cgttcaagca gactcgctta accctactaa 107040 gtacgcttgg agccttatca aaggtacaga tggagaaact tctcacatac acacagctta 107100 cgcttggtct gctgacggta ctgacaggtt tactaaagtt taccctcagg tcaacctgtt 107160 agagaacact aagaacccta ttagctccaa aacagttaga gtgaaaggtt ctacacactc 107220 aaacactgat gatgtaactt tcaaaagcgg tataacacac caagaaagaa aacccaatta 107280 tccaatcgga gaaatgtatt atagatttat gagtacagct gatggtaact tggcttctac 107340 taatctggta aagggggaga cttactatct tggtttagat ttgagagccg aactagctga 107400 gggagattac gttacatgga gagctcagta taaaaaatcc acaggaagct ggattgatgt 107460 tgatggttgg aaaactggag tatccaaaat cgagggagtt acttccgagt ttaaacgctt 107520 tgaaaatgct ttgacaatcc cgaaagatgc tgtaaccctg tacttctctt atcaagtata 107580 ccctaaatcc ggaaaaggtg ttgcaacagg ttcaatagtg gagttcaaga atgctaaatt 107640 tgctttgaag agtgctaccc catacactcc tactcccgaa gaagactatg agaatgctta 107700 tccgtcatac actggaacat actctgactt caatgtgaaa ggtagcgaca accctaagga 107760 ctacacttgg gcacgttcta tgggctctga cggtgaatct gcaaaaacat taattttatc 107820 aacttcttca aatactgtta aaattaatca agattcttct atccaatcag acactataac 107880 tcttcaagct aatatgcaaa atatgacagg aattgctact ttcagcgcca ctccatatat 107940 tggagcaaga ccacaaacag caataacatt gggcgggaca ggaaatacaa gaacattttc 108000 tacttcctct tggaataaaa attggacttc tttggtagtt actgctacat ttgaatcatt 108060 tattgataag caaacaatta tttctgttag agaaggtaaa gatggtgctg attcaataac 108120 tgggttatta acaaatgaat ctgtaacatt acctgctgat aaaaatggta ttgttgagag 108180 cttctcagag gcctcaggaa cgtttaaggt atttgatgga ttatcagatg ttactggtag 108240 aaacgttgtt tatacattaa agaataagtc aggaggaacc tttaatatta atgcttctac 108300 tggagtatat aatatgactg aaatgagcaa agattctgct atagcagtct ttgaagctac 108360 ttataaaaat attaaaattg aaaaaatatt aagtgtagct aaatctaaag caggaaaaga 108420 aacttatacc tatacaaaat attctaattc aaaagatttt ggaaattatg actattcagg 108480 gaatcctaat attgcaccaa taattaatga tgataatgta aaatcattaa ataatagtat 108540 gactactgtt acaaatcatg ggacttatgc aacagcagaa aaaattggaa atgatttaac 108600 tgtaggtgta ggtatgttcc catggtctcg atatgatttt agtaaattaa ctgtaggaaa 108660 acaatatact ttaactatac ctataagaat caatgaatca tataatggag atatttctag 108720 attatttatt agactgagat gtttagacaa agataataat gctctaatta ttattactgt 108780 aatggtgccc aaaaacaccc caaaaggtgt atttacgaat atatcagcga catttaatgt 108840 tcaagcatca acacaagtat ctcattcttg gtatgcacaa gttgggtcta gcacggacta 108900 tagttctaga ggctcaatcg atattggtta tgaagttaag ttagaagaag gtgctgtatc 108960 tacaccatat cagcctaact tacttgatga accgtattat ttgagtaagg tggctttggg 109020 tgaaaatcta attaaaccag aatcacaaca accagttact aatagtaact atcttattaa 109080 aatctataac accaaaccaa tggtaaaagg caagaagtat accatcacac tggaaggaac 109140 taagccagca acacaggttt ttagaccatt ttttacgcaa gctacaggaa gtccatgggg 109200 ttttggtgat ttgaagccag tagaagggtt aactgatatt tggtctgcaa catttaccgc 109260 aagtactgat tcacacccta ctagcccaca agtgcagatt tatcaggtac caaacacaac 109320 tgtaggacaa tgtacaatta agtgggtaaa actagaggaa ggcgacacac gaacaccaaa 109380 tattgattca tataaatata tgggaatcta tgttggggga gaaagcgaac aacctcataa 109440 tccaactcaa tatgcctgga gtcagattaa aggtaacgat tctacgtctt attcattatt 109500 attaagcagc caagtaatgt cattaccaag aggagcaagt aaactagttc ctgaaaatat 109560 tactatttct ggtatggctc aaacaggtga aaaatctatt gagaacatta aatctcgtat 109620 tgttgtagaa gaaactacta atggaactac atggactact aagtatgatg cagacgcatt 109680 aacttatact tatactcctt cttcattaaa cgttttatct gcacgagtta agatgtatgt 109740 tgctggagct aaaaatgtac aattagatca aaagagtatt aatattgtca aagatggttc 109800 gaacggttct aatggttcta atggttcaag tagttataca tggattcgtt attcagaaaa 109860 tgccaatggt aatccaatga ctacaacacc taatagtaaa actaaataca ttggtattgc 109920 tattacccaa acttcaactg cgccaagcgc ctatagtagt tacacttgga gtagaatcaa 109980 gggaagtggc ggacttgtag ctaaagttta ttctaatggt ggaacaaaca ttgaacaatc 110040 tccaattcaa attaatgtag aaacacaatc tgatggagat atagtagaac caaataaagt 110100 aacatggtat atgtcaaaag attcttcagc agaatggaca gcaattactg ctggaaatgg 110160 aattacctta aataaaaaat atatagttgt tgaaccagta gtattcttag aatcaaattc 110220 aattagtttt aaagctcaag tagaatataa ttctatgaca gcttttgact atatgactgt 110280 gaataataaa agcttcgatg caacgaaacc attaatttta cgacaaccag ctgctccact 110340 taatccttat cctggattat tatggctaga tacttctagt ggtagtggtg accctctaga 110400 taaaactaat ccaagcttag tttatcgttg gggtaaatca gccattgaaa gtgtaaatgg 110460 aactgaatat gcacattatg cttatgcaaa tacgccaact ggataccagg aagatggtgt 110520 tacaccagac ccaagtttta gtgttactta taatagtagc caaaaaccta tgtatattgg 110580 acattgcgta acagatacag cgtcagatcc aactactcct agttcttata attggaagtc 110640 aatcactaca gaaaatggta gcgaagacgt taatagtgcc aaaggtattg cacaagaaaa 110700 aatagaaaca acttacgcat ggggatgggt tgttctaagt gcttctgatt tagaaatgct 110760 tccatggcta aataatgaag acggttctac aattattgac tggatttatc aaattactca 110820 aaaaacttct gatgattcta ttgttcaaac tgtagttggt tcagaagata tgtcgaaagt 110880 atatgctaaa actgatgcgg ttgatgagct aagtagtgcc aaccatgaat tatcagataa 110940 aatagaagaa tataataagc agatgcaaga cgcttttgct ggaattgata caacgtatgt 111000 aaacaaatca gaatttgaac aagagatgaa taaattcaat tttggtattg ataaagctgg 111060 tggggttaac ttaattagaa actcaacagg tttccaatgg gaagatacag cacctagcga 111120 tgaaatgtta acaaatccta aatttgaagg aacaatctca actactgtta ctggattaaa 111180 taataatggt tgggtgtata atggcgcagg cataggtcaa gttataggag cagaaagtga 111240 tttcccagaa aacaaaatta tgcgtattgg tggaacaaat tcaccaacta aatacagcta 111300 tagtaaagac attcctgtct cgccaggaca gttaattaat atttctgggc aagtacgtat 111360 tccaaataca atcggaaata ctaaaaatgc ttatctatca attagatttt atgctagaag 111420 tgatccatca tttagcgctg cttcacaaac aattggcaat ggacaaggtg ttattactct 111480 atttacagat aaaacagatt ttgctggaac agtatttgga acagactcct atactgtaaa 111540 aacaaataat attgttcaag atatctggag taattactct attaatataa ctataccaac 111600 tggagcagca tttatgaaag tactattcta taacgattca acaactccta ctaattattt 111660 tgactttagg aaacctagtg tagttctata ttctggaatg tttgatttct ggcataaaaa 111720 tgattcagcc aatgcccaaa ttagacaaat tttaggtgac gaatcactag aagaacttgg 111780 tttaattgct ggttttgaat taaaaggttt aagatataag ctagaccaag atattcttct 111840 acctaaccca aatacagaat atactctaag ctggtatgct aataaaatta gtcgatcagg 111900 gaagagcaaa tattatgtaa aagtattaaa tacaaaaact ggagcagaaa tagctgtttc 111960 agaagcctct gccgatacta tcggatacca acgaatgtct gttacttttt cagtaactaa 112020 cacaagtgca gttactatta gaattgggct agaaggagaa ggaagcgcag atgcaacatt 112080 gtccgcagca ggattaatga ttaatattgg tccttatcct ctctcttggc aatcccatca 112140 tgaagaagta tattctacaa atgttcgaat tgatagtagt ggtttgaaag tattcaattc 112200 tgattacgat ggatataatg ttatgactcc tagagagttt gcaggttatt atcgtaatgg 112260 agaagtttat gaaagagtat ttactttaaa taaagataca acgaaagtta aaaaattaga 112320 agcgcaagaa agatttatga tgaaaccagt acaaattgta tcggttgact ctccaactat 112380 tcaaggctgg gcctttgttg gatacaatgg tgaagatact gactaaaaat aaatggtcat 112440 tcctaattat ttgggagtga ccatttctta tttcccaaga gagaagagga aaaggaggaa 112500 atttaataat ggcaacaagt ggagagaaat atactgcttt tgctcgacat cgtttaaatt 112560 gtagatggtc gataaaccga caagatattg ctggtaacta cacaattgtc accggttttt 112620 tatatttaca atctatggac ggctatggag ctatttcagc ttctgccgtg ggtcaagcac 112680 aagttacaat taatggacaa aaacaaactg agaacgcaac ttctcaatta agtgcgcatc 112740 aaaataaatt attattagct aaagattatc gagtaggaca taataatgac ggttcaaaaa 112800 cgactactgt ttctggaagc tattttgtaa atgttacatt cgcaggagtt tattatggaa 112860 cgatatctgt tgggaacttt aatgcaagtt taccaaaaat tcctagacat agttctttga 112920 atgcggtacc aacatttaca gttcctaatg gatgttcttt ttctattagt agacaatcta 112980 attttaatca caacttaact ttttgggttc aaaatagagc aaatcctagc ttatcagatg 113040 atagtcattg gacttattta acgaacgtag ataatgttgc tacaagtggt aaatttaatt 113100 ttacagacgg gcaattagca actattgcac aaagaattaa acaatttgct ggtaatggtg 113160 gaaatatttt ggggaaggtt aaactatgga caaatggtgt gtccggcctt agtcaacaac 113220 gaacagttca gattgctcat ccagcaccag taacacccaa gttagcaagt tttaagttaa 113280 cagcaggcaa ccaacttgct atacaattag ataattttca atcaaataat aaatttaaat 113340 atgatattac atttaagtgt gcaggttgga ccaagtcttg gagtaatgta actaccaata 113400 aaatagattg gactctaact caagacgaag ttaacaatat acttcaaaga tttactggcg 113460 cctcaaaaaa cggttttgct attgaggcaa attcaaaatt agcagggagc caatatagga 113520 atactgttgg ttctagtggt tctgcagaag ttgatacaaa tacagcctct cccctaataa 113580 ccgaaggttt ttctcatgaa gatacaaatc aggtatctat tgctataaca gggaataaaa 113640 aaatactttt agataaaaat tctactttaa gagtaacagt ccctagttca ataataacaa 113700 ctagagatta tggcaagcca gctagtgttg aggcatcatt tgcaggaatt aaaaaaactg 113760 ttaattatgt agaaggaaat atggttttag actttggtat tattacagga acaacaggaa 113820 atctaactgt aacagctatt gatagtcgag gattaaaagg aagtgcttct tacgaagtta 113880 cggtacttcc atataatatg cctgtaatta actttaatgt aagaagaaat aatagttttg 113940 agacagatac tttagtttca ggtacttcta tttggtctcc tgttgatgga aaaaataaaa 114000 ttttatctgt acaataccaa ataagtggtt cagataaggt agatattcct tttacgacag 114060 cagcaaatgt tttaagtatt acagcacaaa cattaccatt agataattct aagtcttgga 114120 cagttacaat attcattaca gataaaaccg gaacatattc tagatcatat aatattacag 114180 aaggtaagcc cttaatgttt atcgatccaa accgtaaatc tgtctctttt ggggattttc 114240 caacaactgg ggaagaaaga ggtttgtctg gtgttctaga aattgaagca gaaaaatatc 114300 atgatactgg taaaacagga attcaaatga ataatagtga tatatcagga ttaaatggtt 114360 tatttttctc taatgataca atgaataatt ctggagaagg tttgcatttt ataaaatctg 114420 gaaaagatat taattctcct agacatgaag attatgatta tatgtacatg agagataatg 114480 gtttttttgt aaatagtgat tcaagaccat tatttaaggt aaatgacaac ggataccaaa 114540 ctacatctag tttatattat tatgaacaaa acattgcaag taatgccaat tttaaatcgg 114600 cttttataaa ttattctcga tggggtcaat tagtagtggc tgctgtaggg tttaatttat 114660 taaaggatga aggttggaaa gtattaacta aaccaccagc aggtttcaca ccatcgaatg 114720 gaggagctac tacattaagc tctcaatcct atcgaggtaa gactgttggt ttatatgtag 114780 gtaatgaagg ttttgtcata gttcctgcta ttggagcagg caatccaaac ggagatacgt 114840 atagagggac attggtatac tttacaaacg ataattatcc tgtatagaaa ggagaaaaaa 114900 taaatgtata cagtatataa aatactatca gaaaaaaatg aaaaaggaaa ctggtatgaa 114960 aaatatgata tacccattgg cattccagtt ccaccagact gtgtgttgac cccaattcca 115020 gaaagtataa aaaatcctaa atatgatttt gcaaaaaatg agtgggtaga agaccaagaa 115080 gctttaatta aaaaattaaa agaagaaaat caatctttaa gagaaaaaat agaactaaac 115140 gaaatggcat tggtcgatgc tattaatatg ttatctaata tgattattga aaaaacttaa 115200 aggaggtgat tattaatgta tccatattta gcaatttttt acgcaagtct tatcatcaaa 115260 gacccagaag gctggaaagg gaaagaaagc caaattccag ctatgattcg ggacgatgtt 115320 atgaagcttg tagatgaaat ggcaaaaaaa gacgtgccag ccgagtaata ttatttggtt 115380 taggaatgtt agtaggtttt ttactagctc ttctaatctt ttaatctcgg caaaaattaa 115440 ggaggaaata aaattatggc aaatattgat atgagtcaaa ttcaagatgt tttcgctgct 115500 ttggtagcat ttggtggtat cttggcacca atcgtatttt atattattca actaatcaaa 115560 ccttttattc ctagcgatta tactaaatta atcgcagtag ttctaggagg aggtatcgga 115620 gcagtactag tggcaattgc tcctgctctt ggaattgtag gacttaacta cattgttggt 115680 ataattggtg gtttaatcgg tggttatatt gcaactaatc aatttgatac tgctgctaaa 115740 aaaggctttg aaaaaggtct agatgaaact aaacaagaaa aataaataaa ataaatcaat 115800 aggctgtcat tgcacagcct atttttttac attaaagaga gtatttataa ggaggtatat 115860 aaatggcttt tacagatgat tttttaaaaa aaataaaagg tggagcttat gctagttgga 115920 aaaactataa ggtcttacct tctgttgttg ccgctcaggc agcattggag tcaaactggg 115980 gtagaagtgc cctagcaaca caagggaaaa atctgtttgg ggtaaaagga agttataaag 116040 gacagtctat tgtttttcct accctagagt ggactggtag cggatatgta actattaatg 116100 atgcctttgc taagtatcct agctgggatg tttctattct ggaacatggt aaattaattg 116160 gagaagccac taattatcgt gctgctattg ggaaaacaga ccctttagcc caaatcacag 116220 caatttggaa tggtggtttt gcaacagatc ctagttatcc aagcaaagtt atgtcagtta 116280 ttaatgctaa taatttaaga gcatgggact tagatgcttt ttctggtgga gatggcggag 116340 gattcagtgg agatatagat gggggaaatg gttataaaaa cttttctgaa aaccttatca 116400 agaaaaatgg tactacaaga ccagggttta aattagaagc catgcaagca attgtattgc 116460 atgatattca aagtggttct aatttaggtg gaataaggaa tactcttaat tctggaaatg 116520 gtggacaaaa aatgggctat cacgttattg tatcagagaa tgatgcccaa ttagtagtgc 116580 cttttacaga aggagtttat catgcagaga gaggcaaaag cttagttgct ggtatgagta 116640 atccaaataa aaaaactatt tctattggag tagtaacaaa acgttcatta tctaattttt 116700 ctactaattt aaatattaaa ttagctttag ttattgcaga aatatgtaga gcatataaaa 116760 tacctgcaac cgctgtattg ccagcttggc aagtagatgg cgtaaacgaa cctgttagct 116820 ggtataataa tcctttctta tatactgctt ttgtagggat ggtaagtgat gccattaaaa 116880 aaggagaaga tgtaattaca aatcctgact atgacagtgg aggaaccagc ggaggaggaa 116940 ctggcgctga tggtttaata ccaaatggag aaggcacaat taagaatatt attaaagaag 117000 ccaatgattt gcttggatca atgacctatt cttggacaag acctgcccaa ataagaaaag 117060 gcggttttgg agactgttct tcattctgtc aatatctata tcaaaaacat gctaaggttg 117120 atataggaag ttatactgat gcccaatggt ttggaaattg gggtaaaaaa gttaatgtta 117180 aagatgctag agcaggggac ttaatattct ttgctggaac ctatgctact aatatgacaa 117240 ccactcatat tggaattgtt gttggaggag gaaaaatgat tgactttggt tctactccag 117300 gtccaaaatt aaataattat aatgatagtt attggggacc aaaaatctat ggtgtgaagc 117360 gaattttttc tgatgcagat tataaccaat cccaatcagc aggagaaaaa ccttcaaaac 117420 ctacaattga tcctaaagga acatatgttg taaatgttaa aaaaccagtt gttgcaacca 117480 acgctgatgt cggtggagta tctcaaagaa gactatcttc caatgaagtt tatagagtac 117540 aagaaattgg taaaactagt ttacaattag cttctaatga attatgggtg cctaagagta 117600 gtggcgcgat aacagtttca cgattatcca ccccatcctc tcctattggg gctatttcaa 117660 ctaaattacc tacaaaagta tattctgctc ctacttatgt agcagaacca gcaatagaga 117720 aaggaacgcc aagaattctt cctgaaaaaa caagtatgaa tatttacgct gtagagaatg 117780 ggtttgctca agtggattta gaaaaaaatc attgggctgt agctaattct acatatgcga 117840 ctttgaatat tgatttatct gaagaatttg tagaagatat taattttgaa cagggcattc 117900 caacttcaac tactgtaaga ggacggtatg aaccagaaga ctttattaca gtagagaata 117960 gtttgccaat taaaaatgga ttagctgtag ttgctcatcc agatttattg gatattggtt 118020 cagtaattaa tatagaaatt ccatcagcaa ctaaatataa tagaaaagca gttgtggtat 118080 caaataaatt aaatgattca gatggaaatg tattagaatt gatatttaca aatcaaggag 118140 atcaatataa ttttggagga agaactggaa ttattacatt attagaagta ttagataatg 118200 atgtagatat tacaaaattt atgaataatc cagaagatta cgaacaaact aaagctattc 118260 ttccaaaaac tccagaagga ggtatgttag gtgagccaaa gtaaatcttt aattttaaga 118320 gacccaaaag gacagaattg gttggtccga gtataccatg aagatggtat ccgtcaattg 118380 tatacaacaa aaactgacga agagggagaa aattcagtaa gaattaaaac ctatcaagga 118440 tattataaga ttaaaataag tactaaagga caactaataa catttccaat tgattcaatt 118500 gggtaatata taaaaagaat tagagggtat gcaataacaa tgtgcttatc ctctttttca 118560 atacttttag agcaaaaaac aaaaggagga aaaggataat ggaaggattt aaaaaacaca 118620 cttttcgtga ccatcaaggg aatattatac ttcctgagac agtctcagaa atggtaagag 118680 aaacaccaga tagacgtttt gtagataatt cagaaaaaca aattttgact agatttggtg 118740 gcaaaatcga tattgttgat atgttaatta ctaatgcaga ggctattcgt gcattagcta 118800 cacaagaggt tgcattaacc caattaattg ataaagtacc aaataatttc gaggacattg 118860 ttttaactac agatgagcta tcacgtttag ttccttatat ttcacaatta attgaaaact 118920 taagttatcc accagttgta acttctacaa cttcaggttt atcttatgct ttacaaattg 118980 atgatacaga tccagataat ttaaaactta aattcgcaca acaatctatt aatacaggtc 119040 aaccactaac ttatgttaag ggagataaat ggaaggttca accaggagta ccagatgtat 119100 tagttgaatt agcaccaact catattgctt ttgataactc cagaaaaact gttttagtct 119160 ttaacgaagg caaaaatttg gtggacgtac cagaagatgt atttacaaat atagttaatg 119220 gtgtccaaga agaaactatt gctgcagaag atgataatat tccacaagca tttgtacgat 119280 ttgcggttcg tcatcaattg caggaattat tcgctacttc taaaagttta gaaaatacta 119340 tttcaaactt tactattgaa gtattatcac aaccaatgga aggtacaaca agtgcattaa 119400 cgcaagctta tatgaacggt aatgtagtac aaccagtgcg agaagcaaat aaaaaagaag 119460 attatactgc taatactttc caaaatatta catggacaaa tagtttagat actaacttta 119520 ttagagaatg gctaaacgac tctggcgaag cagcattggt tacttatgga aaagctaata 119580 attctattag tatcgcaaag ccaacattaa aagtttatat taaaaatcca tatattggaa 119640 aaacattagt tgctcaaaat gatatggatg gcaaatttaa tatcttagat tgggctatta 119700 gtgaagaaga ataaaagaga aaaaggagga aagaactaaa tggctattta ttataatatt 119760 ttatatccac cattagttcc tattacgcat cctgcctttg aattaggaaa accaactgac 119820 acgtttagac ttttttttga accagcagtt ggtaacaaaa tttctgattt taaaggagga 119880 tttattagaa tcagaaatgc tgagacagaa aaaagtgcgc tattaccagt tggagaagga 119940 tacctagatg attttattcc ttttagaaat ccatttgctg aatatattga tttaaaagat 120000 ccttcaaaaa atataattca gccaactgga tataatacta caatgcctta tgtacaaaaa 120060 aatgctaatg gagaatattt cattgatata aagcaagaag cttttactat gtctggcgca 120120 gaaaaagatg ttagatataa aatgcaaatt atgttaacaa cagactggct ttcttctact 120180 aagccaaatg gaagaggaca tattcaaact tactctagtg atgtacaaca gtatgtaaat 120240 attgataaaa ccacatactt tggaggaaat ttaatttcta agggattatc tgagtggtct 120300 actatttctt taatttctcc tgtaagtgaa gcaatttatg aattacaatt tgatggtaac 120360 aatatttttt ctccaatatt tgaatttgtt ggttctaata tacaagaaaa tattagaaat 120420 aatacaatgg ctaattatct taaagcttat agaattgata tttatagagc taacggagac 120480 gaaaaagaac tctttattga ttcaagtgat tggattatcg gtcaagaacc ttcaaatatt 120540 gaaattagat ggcaaaatgt agtagaatta gaagataaga ataagtatat agttgaatta 120600 tcaatacaaa ctgcctggga cctaagaaaa acatttactt atcatgttac aacacaattt 120660 gaaggaagct tatttagagg tacagttagt gttgaaaacg atcatgataa tgctcgttct 120720 aaaatcaaac taaatataaa aacacctcta caatggggac caaaggaaaa ctttgaaatt 120780 tctcttaacg atagagaatt tgctgacgca aatggagaag tatctgtact tgaaggaata 120840 gacttcttta ataaacaagc tgctatagca ggggaaatga tagtttctgg aattaaccca 120900 ataaaaactt gggaagcgaa agaagaccga tggttcttca gactaaaagg accagagatt 120960 tctacaatta acccctatca agaagaatat ctaatgtatg cccatgccgc tcctcttggg 121020 aaagataaag cttctgaagg tccgtacgaa gatgatatta ttattaatcc tgttattgaa 121080 agtccatcag gagatatata tctaacttac ctagacagtt ctaaaactcc tacaacagaa 121140 ggaggaattg ttggaggtat gggaggtatc gttactattc cgagcgaaga tttaccaagt 121200 tcgcatacct tcttctattt agaagatgaa aatcaacaat tatggaaaac aactgtcaca 121260 attaatggag aattcgttac agaaaaatct catgaaaaag atccaacaga atttttaaaa 121320 cctgtttgtt tctttgattc atatcataat attttagtag tgccaagagt agagacagat 121380 ggaactattg ttcttgatac agtttacgaa aattatgtat tgggcgaaaa cgtccgtcca 121440 atgtatctta atgaatttag atttgttaaa cgagtttatg ctcttgagct tggaagaaaa 121500 actctattaa ctacacaaac ttataaggct tacatgaatg attttaataa gaagttaaat 121560 aagtgggtta aaatacaacc aacaagaaaa tattatatgt atttttcttg tattgatgga 121620 cagataagat taatagtacg agatttatcc gctgatgata gccaaaatag cttagataga 121680 tttacattaa cttattctga aggattcatg aacagtgttg gaataagttc tgatatgttc 121740 ttaacaacaa ctgggttagg ttcagatttc ctaccaaaag ttgatttaag tggtaaggaa 121800 gttccttata caattacagc agatgaacga ggagtcttag gaacggatgt tggaaacatc 121860 tctgctgcaa caacacgttc gagagacttg ttaaccagtg acattgaaga attaaagagt 121920 aaaggagaaa tagaataatg gataaagtag acttaaaaat acaagtaccc agacgattag 121980 aaattgatcc tccagctaat gtgggacctt ctaaattccc tagtgttgta gatcaattaa 122040 aagtaattga atattcaagt gttccttctg ggggacaact agaaaaagac ttacatgatt 122100 attatgctat cttagcgaat gtatcaaata atacagcatc agagcaagac tttgtaaaac 122160 ttaaagagtt gatgttgaga gttcgtaatt atgttttgac ggaagatgat tttaatctaa 122220 tggctgatgc tattagaact acacagtctt atttaaaagc atcaattgaa gcggcagatg 122280 gcaactatga actaatttca attgttgctc aacaactagt agaccaaatt aatgaatgga 122340 gtctatggct tcaagatgaa ctagcaaaag tagcagctaa taaaaattgg ggtgcgccag 122400 taatttatag cgaaaatagc ccaggacctt ctgctcttgg atatttgtgg gctcaaccac 122460 aagacgatac ctatattgct ccggcaatta cttttaaccc tgatgaagac ttaaatgatt 122520 atccacaata aggaggtaga tataaatggc agtaccattt gcttcattta aagaatatgg 122580 taatgacgac ttacaactat actatccctt attaatgact tctccagcta ttccacaggc 122640 tggagatagt tatctttctg atatagcttt aagagatatt tcaattaaat ctatatatat 122700 ggaaggtggt catatggagc ctaatttaga ttgggatggt ggtcgttatg attgggcttc 122760 tggagttaca gaaatacaat cagactttgt tgacgcagga ggagaatctc caacaggtaa 122820 ttttaaccta ttaaagaata cagctttatc aacctttcaa agtactcatt ggacctcagt 122880 ttttgaaaaa ccttatgtag agggttctga tgatattgaa caaccttata attggattta 122940 ttctaaatct gaaccttccg accatagtaa aatttgggta gatacaagag aagaaactat 123000 agaacctaaa tattatcaaa atggctggaa aaattattat gattttaaac aaaaagagtt 123060 taaaggtgag gttggattag tagaaaataa aagaaatgga aacgccttta gaatttataa 123120 agctcctaaa attaatgcta acataggaat cgctcaattt gtaccattaa aaatagttct 123180 gtggcaagga gatgctatta cagttggctt taaccttagt gagaaaactg attcaggtgg 123240 agcagtaatt cgtatagaat tctatgataa taatagacaa ttaattcaaa caaaatattc 123300 tcctagattc tttaataaaa ctgaagaaag taaacatttt ggacatcctt ttatttttac 123360 agaccgaaca aaagcagctg cttttattag agtttctatt cttggagtag aagatcgaaa 123420 aagtgaatta caagtaaata gaattaaagc agaaataggt atggaagata caggatggaa 123480 tttgcatgaa gaagaattat tgttaaatct taatgcagga gaaatagacg ttccaatgct 123540 gaatggagag gttgataaat ctaaattaat ttctgaaatt gcaatcttgc gttctgtttt 123600 gggagaagga ttgctgaaat gggaagaagt aactagacta aaatctagtt cagtacaaga 123660 agcaatctta tatgattatt ttatagaaaa tggaaattac tatcggtatg ctttgcaacc 123720 tatattagcc aatggaatga aaggtgctat tacttcattt tatgatactg taactacttt 123780 tgatggtttc tggttgctag gagaaaaaga tgaacagttt tcatttattt acaatggtaa 123840 aattggagaa atctcctatg taaaacctag agatgttatt caaactattg cagggcaata 123900 tccatatttt gtaacctctt ctgaattaga ttatagaaca tatcaatttt ctggtacgtt 123960 aacctatcac caagatgtcc acaaattact tacttctgat agttattctg tagcaatatc 124020 tccagatcca actattccta ttagttatgt agaactaaaa tatggagatg aaatgttgct 124080 taatatgaag aacgatttag aagaagtaca agatggcatg gtaatgcaaa gaatttggag 124140 aaacaaaatt ctagcatggt taaatgatgg aaaacctaaa attcttaaat cagaagctca 124200 aggaaatatg ttagtaatgt tgacaaatat taaggttact ccaaatgaac caacgtttgg 124260 tttaatttct gattttgaat gtactgttac tgaaattgga aaacttgatg aaaaagcatt 124320 acaaaaatat aaacttcgta aatcttctat tatgaaggat gagcttgtaa aagaagctat 124380 gaaaaatact tcgttctaat aaataaaatt aagctgggga acttgtgata gtacccagtt 124440 taattttttt atataaaaca taccaagagc aaaggagaga aaataatgaa tgaaaatatt 124500 aatgttaagc agtatcctac ttcttttcta gaagagcttt tcgagcataa tattcgagta 124560 gtgcatacta agatacagtt acttaattgg gacgaacaag ttttaaaaga aatacaaggt 124620 attgtaaaaa gcggaagtta taatgctgac ggaacaagtc gagtaagacg aaatttatct 124680 ttaaccttta gtgtaaaaga tagagacgat gaattagtct ataaatatct aactccagat 124740 aaaaaaataa aattatatat aggattggaa aaccaaactt ctcgttatca agaagatgaa 124800 attatatggt ttaatatggg gatttttatt ttaaccgaac catcttattc tcattctgtt 124860 gatacagcga ccatatcaat taacgcgcaa gataaaatga ctatgctaaa tggtacatta 124920 gggggtcgcc ttccagcccc agtaagtttt gtagaaaaaa ggaatggtaa gaattattcc 124980 ttctcttgga gagatatttt cttaagcgca gcagtaaatt ttgggaatga gaatccagca 125040 aaggttatta ttgattctgt cccagattat ataaatgaat atacgcaagt aaaaaatgtg 125100 gctggactaa aagataaatt tattcatatt aacgctcctg gagatgtaga aggagaaaga 125160 attattacta gggcgtggag tccaactgtt ccagaaacag aaattaattt ttcaaaggga 125220 gatagagtct acaaattacg cagatttggt ccacctgacc cttcagcagg aacagtaagt 125280 acccaagaat cttatcaaaa gaatgtaaat gaaccattaa cctctatttt tgaagatatt 125340 gtagaagcta tgagcaatac tcatgaattt ttctatactc gtgatgggga tttaatgttt 125400 caacctatca agaattatgt taatgaagta tttgaccctg aaaaagatac agatttagga 125460 tattttgctt atgaattaga catggatgat tttatcccta attatttagg tctccctttt 125520 acctataatt ttgccgataa aaagactatt attagatata acaacaatcc tagttataca 125580 aatatcaaaa atgattttat agcaactaga gaaacaggag aaattttaga aatagcaata 125640 gaccataaac caacaattag agagattaaa gaatggtttg ttgggattgc agaagatttt 125700 aatatggatt ctccagaaat ggatttttta gcaaaagatg gaagaagaag ggagccatat 125760 aaccctcaaa ctaatacagt tccttttgaa tttaaagaag ccacagcagg gaaaccaaca 125820 caatatattg aagttccttt agataaaatc ccatggcaaa ttggtttagg acttaaaaat 125880 tattttatta gaaatatcta tggcgcttct actgcaagag ttctgcctag atggggtaag 125940 gaatgcgaat caatgatttt taaatggatt gcatctgaag ataaaaaaac tttgttgcca 126000 aatactggaa tttttaatcc atcctttatt tctgtcggaa ctccatggtt agcaggttat 126060 cctatggctg caagcgcaaa cacagaaaat gatgttgagt ctttagataa acagaatcct 126120 atttttactg acaaaggtga ttcggcttat tggacttact ttcttgatat tataccaaca 126180 gaatctaaat tgggtaaata ttctattgag ttaattggta aacgttctat tggtgtttct 126240 aataaacagg caactaccat ttttagaaca aaccctaaag aattaattgt tattacagaa 126300 acagaattac aagatttagg cggagatatg atattagata atttaaaagc acaaggagaa 126360 gcttatgcag taattagaga tatacaagat caattcttta tgccaacagc aataacaaaa 126420 aataaaaatg ttatgcctta tagtgctata gttggagacc cagggcggtt tgaacaatta 126480 agatatatgt ttgcagaagg caatggaaca gttcatgaat atttagctgg tggtaaacat 126540 actggtacta ttggggtaaa taagaatatg caagatcaag ataagcctgg atatgttaca 126600 atttctgctg gagtatacac caatccagtt acaaaaagac agtttactca aacagatttt 126660 gcaaccataa atactccttt tggtagtgaa aaagatttaa cggaatatgg ttttattgct 126720 tatctattaa acaaaaacag ccgctgtcct gatagtggaa atcataatta ttttgctgta 126780 gttaaaaata taggtactca atggtactat gctaaacaaa ataatggaaa acctgcatgg 126840 actaaattta attgggataa aaccaacgac tttttggtag cagtattaag taaaactgtt 126900 tataaaggaa gtgcagattt tcctgattgg tcaagtggta gaattgatga ttttcaagag 126960 ttatttaata ttagagattc taaaatggat aatttattta ctattgatgg agcggttgat 127020 ttattttctt ctgttaggaa tttaatctat caacatacta atacttcaga tgtagtaaca 127080 atagatgtat taccagttta tcatttagaa ccaaatacat taatttatgc agaagatgaa 127140 atttcaaata tatcaggaat gtttatgata acaggatatt ctatacaatt aaacacagaa 127200 ggaagtccaa caatgaatat ttctgcaata caaacaaatc cagctatata aaaaaagaga 127260 cctatgtggt ctctttttta gttccaatta atttcttggt gtgaagcata atagccaatc 127320 ataattgcat ctgcctcgtc ttgtgttgca gtaatatcaa atagttctaa tactttagcc 127380 tgagcatttc ttttttgttc tattctattt ttacctttaa tcttagcaaa tcctttccat 127440 tgagaagcgt agataaaatc aaaatgaatt tctggatgag cctctaaaac cccaacagca 127500 agagcgcctt gtagttgagc taattgttta aaagtagttt tattttgctg catttgaata 127560 tcttcaaaca ttatttttat ttcttcttta ggatattttt ctttagtata ttcaattaac 127620 gctaatacat ttgctttctg ttttgaatat cttgagaaat catctccttc agcagaaaat 127680 tttccatagg caattaattc ttgattctta taaacagaat aacctgttac aaccgttgct 127740 tggtctaaac ctataaaaat catattaaaa cacctcccta ctaataaata gcaaagaggt 127800 gagacatgtt gtcttttttt gtcctatata agagaagaat atctttctta attattttac 127860 tcctgtactt ccgtgcccgc caagacgttc tccttgagca ttatcgtcat ctaccaacaa 127920 gaattttgaa aatactcctt gtccaatacg ttctcctttt ttaatataga tagtttcagg 127980 accaaaattt agaaattgga acataatatg accatcatta tcaggattgc tatagtagtc 128040 accatcaata actcccacac catttgttaa aactaagaaa cgttttaatg gatttgaaga 128100 acgattaaat aaagctaaat attcatctgg ctccatataa gcttttactc ctgttggaac 128160 taaaactggt ttaaattgct tacttaaagt tttaatttct gaaatagctt catcacttaa 128220 taactctttt agaatttcat tgttttgatt ttctaaattt agagtattaa ataattctgt 128280 ataagaggca gggtctgaag ctaattcttc taaatccata cccttatctt ttaaaagctg 128340 agtaatcttg gtaaggaaat ctgtaacttt agaaaatagt tctaaagatt caacttctgt 128400 taaatgttcc ataacttgac taattgaatc tggatcttgt aaatcaatat taaacccaaa 128460 tagatcttta gaaaggatac ttgaaattga tttcttatga atttctttaa tatcccaaac 128520 tgaaggaatt gcaatatcat ctgatgcttc aaaatcatat cctgctgcac gagcagttgc 128580 tcgaacaggc aggttaatat ttttatcttc aaaacctttt gctacttcaa atccacgtac 128640 ttttgtcata attatttagt ctccttttta tctttttgcg taaaatcttt aactgactca 128700 acaatccaat cgttgctaac taacttaaaa taagtaagtc caatatagag tccaagctct 128760 ccatgagaag tatcatcaat atctcgtaca tccatcatat cgatcatatc agcttctaca 128820 atatcatttt catttttatc tttaatattt tcttttggtt ggcaaaaact agaatctttt 128880 aaataaccta agatatgttg agccacttct ttatctgtag actcagttcg atcaaaaact 128940 acattatagt taatatctcg tgaattaatc caaaaatatt ttccaaaaga ttttctaaca 129000 aaagttagaa cggcttgata aatatcttct tctgataatt tcggtgcgtc ctttaaagct 129060 tgtgtaatta aatccgattt tttcattgtt tgtccattat ttttcttttt catattaatt 129120 attctccttt aatttttcca taattttatc ttgtaaagca cttaatgcca tccatacaaa 129180 gggtgtatct gaaaaatagt aagtatctgg ctttctatca atttcagcat aatctttttt 129240 atctgcttca attcgtcgtt taatttcatt tacgtcagga ttcttttgtc tgccctttgc 129300 tcgttccatt cgaatctttt catccgctac aatttctatt aacataacat ttgtatcctc 129360 tgatttacct acccaatcag ctacttcaaa tgcagtctct ttttcacaag tcattaaata 129420 gatagattct ttatctcgag cctgagcagt aataccaaag taaacaccat aattccaatt 129480 attaaatacc ttaggaaata gaatttcttt tttttctact cctctttgaa aaacttcatc 129540 agttaaaaat tgataataag attcttcttt gtcggaacgc ttgggtcttg ttgttggtcg 129600 tttggctaga taagcatcta caccaagaga gccaagtaat ttccttgcaa tataatcttt 129660 accagctcct gtttgtccat ataagattac taacatttta tctctccttg ttttaacttt 129720 ataattaatt ataacatatt atatattatt tgtcaatctt tcgccaagat cccagtttta 129780 agtactgaga cctttaacgt aagataatta acctcgtcgg attaggctgc tcgttctgtt 129840 ttcaaaacaa tacttccatc atcacgagtt tcagtaatta atccaatctt tggagtcgct 129900 gtatcattat atgctctagg aataaattga tcgtcacgtc taaaaccagt aataaataat 129960 ttgtttcctc tttcaaagaa agatttttct acaacagttt tcttccctgt tgatgttctt 130020 tctgagattt gtttatccca gtaagaaaaa tctcctttgt acattttaac taaaacaaca 130080 gttccttctg gagttaaaac atcgatactt gctttaacct tatttttacc aataatagtt 130140 ccaacaattc tatttaattt tagcttagaa aaacttgcgt aaggattgat tttttgtgcc 130200 tctcttggaa gaagctcaaa attatcaatt ccatatctat ctacatccac acaatttaat 130260 tcatgttctg tataatagaa gttcatagat tccatttccc atcttgcata tgaatcgtga 130320 ccaccatatg aatcaaataa ttctaacatt tcatcaaaaa atatttcttc taataaagac 130380 tctttattct ctctcaacca atttctaaca gtatccattt ttttgtcata aaacttactc 130440 catctatctc gactcatcac agtccaataa cgatcctcat cactaaacgt ttcaataaat 130500 tcatctaaac caatcgaaga taaaaagttt tgcgcccggt catcaagtct aacaatttgt 130560 ccgctcttgg ttagaacttc atttatataa cggttaaact cataaattcg tttttcttct 130620 tgtaatgatt cagggaatct attaattttc ataaccaatg ggacttgtgt cattgtgaga 130680 ttttttctaa gccctgtcat cattttagca tattttgcta aagtaatctt tctatcagga 130740 ctatctattt ctctaaaagc tcctgcctta attaatgtta gcatttgcat tttatttggg 130800 tttgtttttc tgacaaaatc tgctaaagaa ttaaatggtc ttccctcgaa aattttctga 130860 ataaattcat aaccaatccc agttaatggt cttaaaccgt aagaaataaa attaccttct 130920 acattaggag aaaagtttga ttctgattta tttatatcta ccatttctac tttaatccca 130980 tgtttaatag tatttcctag tgctactgcc attttagaat aatctgttga agttcctgtt 131040 tcatctcctg agttgacaat taaacaacca cagttccaat aaatagatgg ataatgatta 131100 aataagttta attcttgtag tgcaataatt gtatatccaa tcgtatgcaa aattgagaaa 131160 gaatcgtgta tatcctatat tactataggc actgactata tcttaactca cgttatgtga 131220 gcaatggcta tttcggcatt taaatgactt cgtttcctaa aatcatgcta cgtatcaata 131280 gtagccctac ttccctgctc ttcacttagg gaatagtcga tacaggtttt ttagtatatg 131340 ggcgaatagg ataagtatca ctcaatcctt ttcttagttt tccataatta atttttttaa 131400 cggtagaaac acttatacca agtctctcag ctattttcgc aaaagataag tttgtttctt 131460 ttaataaaac aattaattct tcgtagtcag aagaagttct ataattttta attaaaggat 131520 aactccaatc tttattaaaa tatcttaatc ctatattgat attagaaatt aaagtttttg 131580 aaatagagta ttctttggaa atttctgtat aactaactcc attttctaat cgctctttaa 131640 tttcttctac ctgagaaata ctaagagaac cttctataat actttcgttt cctccacgat 131700 gaatattgta tccattctta ggagaagtag tattatattt attaatatag tatatttctc 131760 tttcatcaag ataatcagtt tcctccaaaa taacatgaac aaagttttca tatccatatt 131820 ttctaaaagc tctatacaat ggataattat aacatttagt tttttcatta aaagccattg 131880 agaaatgttc tctctttctt cgctctatat tatttgtttg accaatatat ctttttccgt 131940 taaacatgtt tatatacgca tatatataac ccatttttat atcctcctat tgaggatacc 132000 atactaaaac ttcccacggg attgtcctca gcattacctg gtaggatttc cccgttagca 132060 cagtatactc attttgttaa aataagtaaa actgtacccc acttgattac agtggaaaaa 132120 ccatttaagg gcgttcttat tcaccccatt tgaacactaa ctgctgaatc ccaaatataa 132180 tgaattgttt catttgaaca tactgcttgt tcaaaaattt tagctctaag ttctggaatt 132240 ttatccatta gcttcttacc aatgatttta cgtgctgcat tagcttctgt ttctgtaaaa 132300 ttacaaatgt ttttatccat taaaatcaac attaattctt cctgtgtatt tggaacccca 132360 taagaagata agaaatgttt ctctaggata ggaatttcat ctgctggaat attatatttt 132420 tctaattctt cataccaaag ttggatattc tctttaaatc ttgcatattt ttctagtggc 132480 atttcaccat tttcagttgc cataagcctc atcaaagaat tcaatgctgc tgcatctttt 132540 acatcttctg gtttaccctt ccttacagct tcttttccta caggtgtatc aagctgaaaa 132600 atatccaaaa ctttgttatc ccatgttggt tcccataatt ctttgctata atctaataca 132660 tcgggatgca atgtattaaa atatgtttct tttaaagaac ctttccattc cattctattg 132720 tctttaatca ataaatccat tgtttgacga attttatcta aagcttcgat agacaacata 132780 tccattttta atgctcccat atcatcgcta tcatgataat cccattgagt tacttctaag 132840 tcgttaggag ttttcatcat agcattaata tctgtataag gatgattaaa aatataaact 132900 cctgaagcat gagaacctct actttttacc attccttcaa tagctaaagc tgtttctaaa 132960 taattttcgt atttattaat ttcattaata aattcagaaa ctggtttacg acctttttct 133020 ggattcccat aaaccatatc ttttaaattc caatcaaagc cacgttcatt tggaatcaaa 133080 ccacttaaat aacttgaaac atcatcactt ataccaagtc ctcgtgcagc agtagctaat 133140 gctgattttg ctttttcagt tccaaaggtt gcgatattta aaactctatc atgaccaaaa 133200 aagtttttta acgccaataa aatttgttct cgtttagaac cttgtgaatc tatatcaaca 133260 tccatgttct ccctagtttt cactagggca cagactatat cttccttacg tatatacgta 133320 aagtgaatgc acttcggaat ggtgcttatc tccatcccta cgagtataaa actctagtcg 133380 ttacacgttc tttatttcaa agtctccagt ttttctatat ttttctaagc ttgtaaaact 133440 tgctcctaat tcttgagctt ttctacatgc ttcttttacc gaaccatata ttcctaaaga 133500 cacactattt tgaaaaagct caacttttcg aaaatttcgt gtggatccaa tcccactagt 133560 ctcataagac ttatttatat tttcttgtgg agagacccac tctaaatttt caacacaatt 133620 gtttaaacaa tttccatcta tatggttaac ttgaggaaga ttattagggt ttggaattaa 133680 agtttcagct attaaacgat gtaccaaata ttttttaagt ttcccatttc gagacaactg 133740 gacttgtttg taaggacttg ttgttccaga ccaagttgct aaaatttttg gttttgtcat 133800 tttaaagctc caaacatttc cctctctatc taccttataa tctccattat atccgtcaat 133860 atcatatagc aatttatcat ctccaattaa ttatttggag aactttgaaa taaagcttcg 133920 cacggtatta tccagctatc ctattctcta ggaccttaga ctctcttaga aagctgcttc 133980 gtctttgtta tatctccacc atctcgttgt aacgcttgtg tggaagtctt attcaacttt 134040 taccgttagc acgatttatt taatcgtaca ccctttagca aggttcacat tcaatgccca 134100 ataagtttag gcaattctgg acgactttca gctagatgtc tccaactcat atctcctaca 134160 tatgggatag gatctacttg tgtaacgtcc aacaaccagt ttgacataaa tcccatagct 134220 gaaccacgag agactccaac caatgaatct ccttcatccc aaattaattc aataattttt 134280 tgagctgtat tataataaga tgtcataggt tgttttaaac gttcagaaat ttttactaat 134340 gtagtagctt caatttccaa ttgttctaag tatctttcat ttaatacatt cctaatttct 134400 aattggttta gacaagttct gctccaatat aaatcttggt cttcatcaga atttaacaac 134460 attgttaaat atttatattt gcttaaatca atatcattat atgttttact taattctggt 134520 acatcaacag gaacatatgg aatttcttgt tccttaaaca aagaataatg tttgattcca 134580 cttctaattt tttctaagtt atctaacatt tcctgaaatt cttcttctga aaaatctaat 134640 aaaaaatatt ccttaacttg ctcaggtggc atcatataag cagtataata gaaatcgtca 134700 acttctcgtt ctgcttgctt actattcaaa taagctttat gaatctctct gtctgcttca 134760 gtaagataat gactatctgt actaaataca actggaacat tcattgcttt cccaagtttc 134820 caaactcttc tatttacata tctttgttct tctgaagtgt ttggagcaat ttctaaataa 134880 aaatcttctt cgccaaaaac ttcttgattc cacaaaacaa aatcaacaat tttttgtttt 134940 tcttctaaga ttttttcttg attaattttt tctccaaatg ggacttcttc tgccgcttta 135000 attttcaaaa aagcttgagc ttcaaaactt ccaagacaag ctgtagaaga tacgatatgt 135060 ccttttccat tttcttgttc cataatttct tttacttgtt cacgagtaat cggagttcgt 135120 tgtaaacctc tatcccaata actattttcc caagccttag aagacaacat tcttaaaatt 135180 ttgtgtcctt ttttatcttt tgcaattaat aaatgatgat aatatttttg tcccatttct 135240 aagtcttcga ctaaataaat ttcatctcca aaaccaattg taaaagattc atggtctata 135300 cgaccttctt tttctaattt atctctataa tgcattaatt gagcgtgacc agataatcct 135360 tcgtgatctg ttaaaacata accacttaaa cctaaatcta aagcataatc aactgattgt 135420 ttaatatcat taattgaatc agcgaatcct aacaatgcat tactaaaact actatgatta 135480 tgaattgaat agtatcccaa taaatataac tcctttcata attccttata tattaattat 135540 atcatttaaa atataaaaag tcaagtaaaa taattacttg accaattcat cataattaaa 135600 ataacatttt ctacatacag gaatatattc gttatctcca attaaaattt gttcacctct 135660 ataaatagct tctccattaa ttgtacgtaa attcatagtt gcttttttat cgcataatgt 135720 gcatacagtt tttagttctt ctattttatc tgcaaattct aacaatgctt gagaaccttc 135780 aaataaatta ttttgaaaat cattttttaa cccccaacag aggacaggaa taccgccctc 135840 atctactact ccggctaaag taattacctg ctctcttgac aaaaactggg attcatctac 135900 taagatagca tggactttct cttttttagc cttttctaaa atttcttctc tgctatcatt 135960 tagatactct gcctcaatat taaaaccagt tctagaaaca atctttttaa aaccgcttct 136020 agtgtctaaa tcagaagtat aaattaatac atttttgcct tgctgagcat aattataagc 136080 agttgtaatt aaagataaac ttttccctga gttcataact ccatatttaa aatataattt 136140 tgccatattt atttctccca aattccacta tttttatcat aagctaatac taatttttct 136200 gaagccctag taaccattgt gtataaccat tttctatgat tttttccatt taatcgttct 136260 tcgattccta aaaccttaga atattcacta ccttgagatt tatgtgtagt aatacaatat 136320 ccataatcaa tataattaat tctagcatca ccttttgcaa actgatttac aatataagat 136380 ttttggtttt ctttaataaa agggagcata tcatacttga tagaaaactc tccaccacca 136440 aattctggaa caaaacgaac tcgagcgatc tcaatatctt ttcccattgc atttcccatt 136500 aatttttcac ttttaaatac accactagaa aaattaactt cacctaacat accattaata 136560 agagggaatc cttcttcgtt taaaatatcc caattattat tagttacaat gattttatct 136620 ccttcttgag gagtaggtga aacaaaaccc ttagctaaac ggatttcatc attgtaaagt 136680 tttctggtcg cgtttcttcc acacaaaact tggtctgccc ataaaagcat acctacagaa 136740 atctcttctt ttggaaccat cttaacaaaa tcatcagaaa aaggttttat aatttcccct 136800 tctcgaatca ttttagataa ttgaataata ctattccctt cctcttggcg catgatttca 136860 tctaagaaaa tgtgaggatt ctttaacata ccattatctg ctcctacagg aggtagctgc 136920 cctgggtctc caagcattat aatgtgaata cctaaatctg ctatatcttt caacaggtct 136980 tctggaacca ttgaaatctc atctattaga attaatttaa ttccttgata gttttcaata 137040 ggaagctttt gatgaataaa attattatta attttgatag acttgtatcg caatttgtgt 137100 aaagtttgtg ctgctgggaa tcctgtttgt tgaagacgat aagctgcctt acctgtaaaa 137160 gctccaacca tatattgtaa tggtgatagc tctagtgcat taataatttt ttttgctaaa 137220 aaagttttac ctgttccagc aaaaccagcg attgtagtat aacgctctcc atttttatat 137280 cgttctacag caatttctaa accttgtttt tgtttacttg ttaatgacat ttaatcattc 137340 cttttttatt cattatattt atattatacc attataaaaa taaaaagtca agttatttac 137400 ttgacttttt atttcgttta gttttaattt gttcccaagc aattaaagca tttttcattt 137460 gacctgggta gcgagttaaa tattctgaaa caaattcttt tttggttatt ttgcgtttca 137520 tatctatcac tccttaaatt tatatgtaag ggggggtggt tttaggaaaa gaaaaatagg 137580 tagaaggtaa atatggttca attcaaaaaa tagcatgaac catatttttc tcctagatta 137640 gcgtccgtag acgataaaat cctttaatat tagaaaatgt atttttgcgc tgatttcaat 137700 tctaaatctg caacaaacat ttgtaggaac gtacgacctc taaaagtact cattttaata 137760 gttccaacta catcaatttt tgattgctgg tttttcataa gtttttgagc tgtttcagtg 137820 ctaacaccct taaacttaat aaactctagt cccccagcag aaaatgttat cttgtgatta 137880 tcaaattctc cacccacaac tatatcatct tttttaacag gaacatctct taaaacaaag 137940 gtaggttctt ccaaaccttt tccccaaaac gtagcataac gagtaatttc tgttagattt 138000 tctttagaca tttgctgcca agaaatatca aaatctacag aatatattgg ttcaaagaca 138060 atttctgata attgtttatt aaaatattct acaatttcat cctttttgtc agcagcaaaa 138120 gcaaatccat gagctccctc atgaccttct gcaaattcaa ataatccact ttcttgctca 138180 aatcgtttta attctggtaa atcagattta tcatctccac gagctgaccc accaaagaaa 138240 gtttttccat ctttttcaaa atacgttcct actaaagttg gcttcttata ttttgaaaca 138300 aattgattag caattaatcc tgtaaaggtt tttggaaatt cattttttgt atctaaaatt 138360 agaatctggt tgtcagttaa tttattttct tcaatttgag tttctaatga attaaaggca 138420 ttatctctca atctatcctg tcgagcttta atattattca ttttccgaac aactgcttca 138480 tggaatactt ctgtatctcc ctctttagct ccacgtttag ttgagggtac atcataaggt 138540 ttgtcactaa ccatggcttt tgtaacttct tttttctcat ccattgttcc tactcgtgtt 138600 actgcattaa tcattgggac aatagaaaaa gataaaccga ttggtgttac ttgtcctcca 138660 agattaaatg acttagcctt gtataatgct tttaaaaaag tattgtgaat atttctcatt 138720 ccttgctgaa caatataaat tgtttctaaa gtattaattt caatcatatc tcctacaatc 138780 ccaatagcag caagatcata gtatttttct gagtggtcga agccatacat tttatcatac 138840 aacgcagcaa attttaaaca aactcctgca cctgacaatt tcttattagg gtaatcatct 138900 aaagaaatat ttaccattgt agcatattct gaaaatttag gtgcatcgtg gtgatccaaa 138960 attacaatag gtatatttaa cttttctaaa atatacttat gaacatcata ttcatttgaa 139020 gaacagtcag gagctatgat taaatctact tcatgttccc ccataatatc ttctactgtt 139080 tttacaggaa ttccatgttc tttgttctct tggaaaataa ttattattct atttgttatt 139140 tcttctttat aacaatcttt taaataatta tagataatag ctgtagatgt ataaccatct 139200 gcatcagggt cttgaattaa taatatttta tcttttgaat ttacgatttt gtcgtgaata 139260 atttcagcaa cctctttcaa attttttatt aaaagaggat cgtgcaacgc tgaatcattt 139320 aatctaaaaa ttttatctgg gtttattcct ctatttatga aaatgttaat aatattgtca 139380 tcattttttc caattagttg cgttctcaat atatatcata ctcctcttcg tctgttccta 139440 tggaatccca tagctctaat ttttctacaa aattttcatc tatctctttg tatgtttttc 139500 gttctccata taattctgca aaaacttcaa aaccacaatc tactggagaa tctttatatc 139560 ctgaaatcat attagtatct aaaataaaac ttattttaat accatcttcg ttagttccaa 139620 accgttcttg tattgtttta aaaattccta ttaagttatc atattctcta tcatacttca 139680 tagattgaaa ttgtctatca aaagcgaaaa taatttcttc tacacctaat ttttttaatg 139740 cttcatattg gtgtcgtgaa aagtgcatac caaatgctgc tacaccaata tttttctttg 139800 gggataataa atcatccatt ttcatgaccg atttctccgt attattctac tatttctagt 139860 agtactgact atctcttgtt caaacctaag tttgaatcac catttcgaac tacgtatcaa 139920 tagtagccct actcccctca aggggatagt cgatacaggt tattaatgtt ttttcttggg 139980 tcttatagga aagtttgaat agctactttt tctaactccg ctgtttattt tacttacagt 140040 tgtttgactt atattatatt ccaatgctat gtcgtcaaaa gattttattc catcttttat 140100 ttcatcagca attttatcaa cttgttcttg agtaagtttt cttcctcttg tcccaatttg 140160 atttttccta attggatatt caatatctga aaactttctt cgtttaccat tatttatatt 140220 atctatatga gcaaacgata atccagtttt ttctgatata tccttatatg ataagtcagt 140280 ttttagaagt ttaataacct ctaaaataat ttcatctttg taagttattg tcggaggaat 140340 acttcctcca ggagtttgat tgtaaccatt tctaaaggta tcatatttag aaatataaaa 140400 catttctttg tcgtttaata gacaattatc aatattttcc tctagtataa ctaaagaata 140460 atatttttct ccatgttttc taatgcctct gctaagagga tagttgtatt ctagaacctt 140520 ttcgttataa gggtcgtgtt taaaatgttt gttatgtctt tgctccaaat taacagtttg 140580 accgatatat aaaatctttc cttttggatt tgcatatcca tatataattc ccatatttca 140640 ttcctcattt ctctttcttt aaagaaatga ataatcagga atgaaatcta cattaatctt 140700 cccacgggat taccatatca tttctgactt aggcttcccc gttagctagc ttatgctaac 140760 cccactgatt agtggaaaga tgaactaggg cgatgttgtt caccctcaaa aacaatagct 140820 tttctttctc ttctaatatt gttagcatta aaaaataaac cgaataaata aaatgatagt 140880 ggtgaagaat acagtatccc ttcacgttca acgggtctat attttccata ccgttctatc 140940 atttcgctat tagctattct ttgtcgtata cctaatagat ttccttcttc gtctaaatga 141000 ggaaacatta aaccagttgt tgcataatta tatctgatat tatatttatg ttgagtttct 141060 gctgatatac cttctcgttc ccaatcattt acaataattc taggataacg taaataaggt 141120 tctttatcat aaaacataaa acttggttct tgatattcaa tatctaaatc atcattatgc 141180 aacatatcat tatcgcctaa agtcataaac tcttgtgatt gaatataaat atcaatagcc 141240 tcttctagta aaagttcttc atgatattca ttcatataca ttttagataa taattcaaaa 141300 atatcaaatc taccacaacc agtgtaacaa ttaaataatt ttgtattatc ataataataa 141360 agtttatgac taccctcacc tttatgatta tgacagacag tttcaaagac taattggtgt 141420 tcatcaaccc aattaccttg aataccttgt cctaccaaaa aatcatatac ttgttgaata 141480 gacattccag cttttatagc attcttatca cgtccatatt ctgccatata cctacaacct 141540 acttttttaa ttatttttta tacatttata ttatatcata ataaaaataa aaagtcaagc 141600 aattagcttg actttaaaaa gcactttttc gatcatctac agtttgatta cgacgcttaa 141660 catttttttc tgctgctttt tgaaccttaa tagaagtagc atcaattcca atagggatat 141720 tattactatc tgtaataaac aaacccttac atctacaagt tcccaaatct acgtcgcacc 141780 acaatttaac accagcatat ttacctcgtc gtactttgta aaagctaata atatagtttg 141840 gacgaaaata tccttgactt acaaatgttt ctacagcttg ttcatcaata ggtctaattt 141900 taactgcaat cattcccacg tcaatacgat ccgcaatagc tttagatcca cgaagcaaat 141960 tttgattaag ttctccctca agataatctc cattca 141996

Claims (9)

  1. 장알균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 EF1.
  2. 제1항에 있어서, 상기 박테리오파지는 수탁번호 KFCC11692P인, 박테리오파지 EF1.
  3. 제1항에 있어서, 상기 장알균은 엔테로코커스 패칼리스(Enterococcus faecalis)인, 박테리오파지 EF1.
  4. 제1항의 박테리오파지 EF1을 포함하는, 항생용 조성물.
  5. 제1항의 박테리오파지 EF1을 포함하는, 사료 첨가용 조성물.
  6. 제5항의 사료 첨가용 조성물을 포함하는, 사료.
  7. 제1항의 박테리오파지 EF1을 포함하는, 소독제.
  8. 제1항의 박테리오파지 EF1을 포함하는, 세척제.
  9. 제1항의 박테리오파지 EF1을 인간을 제외한 개체에 투여하는 단계를 포함하는, 장알균에 의한 감염성 질병의 예방 또는 치료 방법.
KR1020170169607A 2016-12-23 2017-12-11 신규한 장알균 특이 박테리오파지 ef1 및 이를 포함하는 항균 조성물 KR20180074578A (ko)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022264035A1 (en) * 2021-06-15 2022-12-22 Ferring B.V. Bacteriophages against vancomycin-resistant enterococci

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022264035A1 (en) * 2021-06-15 2022-12-22 Ferring B.V. Bacteriophages against vancomycin-resistant enterococci

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