KR102313982B1 - 프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화 - Google Patents

프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화 Download PDF

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KR102313982B1
KR102313982B1 KR1020167021588A KR20167021588A KR102313982B1 KR 102313982 B1 KR102313982 B1 KR 102313982B1 KR 1020167021588 A KR1020167021588 A KR 1020167021588A KR 20167021588 A KR20167021588 A KR 20167021588A KR 102313982 B1 KR102313982 B1 KR 102313982B1
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펭 인
사리트 아가스티
시 첸
랄프 정만
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프레지던트 앤드 펠로우즈 오브 하바드 칼리지
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/601—Detection means characterised by use of a special device being a microscope, e.g. atomic force microscopy [AFM]

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
KR1020167021588A 2014-03-11 2015-03-11 프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화 Active KR102313982B1 (ko)

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US201461951461P 2014-03-11 2014-03-11
US61/951,461 2014-03-11
PCT/US2015/020034 WO2015138653A1 (en) 2014-03-11 2015-03-11 High-throughput and highly multiplexed imaging with programmable nucleic acid probes

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KR1020217032767A Active KR102382892B1 (ko) 2014-03-11 2015-03-11 프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화

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US (5) US10294510B2 (enExample)
EP (4) EP4245859A3 (enExample)
JP (3) JP6757255B2 (enExample)
KR (2) KR102313982B1 (enExample)
CN (2) CN105980855B (enExample)
AU (2) AU2015229406C1 (enExample)
BR (1) BR112016018314A2 (enExample)
CA (1) CA2932943C (enExample)
IL (1) IL246962B (enExample)
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Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
CA2919833A1 (en) 2013-07-30 2015-02-05 President And Fellows Of Harvard College Quantitative dna-based imaging and super-resolution imaging
US10294510B2 (en) 2014-03-11 2019-05-21 President And Fellows Of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
EP4273263A3 (en) 2014-07-30 2024-01-31 President and Fellows of Harvard College Systems and methods for determining nucleic acids
JP6858744B2 (ja) 2015-08-07 2021-04-14 プレジデント アンド フェローズ オブ ハーバード カレッジ タンパク質−タンパク質相互作用の超分解能イメージング
US11079388B2 (en) 2016-04-26 2021-08-03 Ultivue, Inc. Super-resolution immunofluorescence with diffraction-limited preview
WO2017197147A2 (en) * 2016-05-11 2017-11-16 Atkinson Robert G Molecular beacon comprising prefabricated components and associated products, processes, and methods of use
EP4047368A1 (en) * 2016-05-19 2022-08-24 Toppan Printing Co., Ltd. Method and kit for target molecule detection
JP2019526034A (ja) * 2016-06-02 2019-09-12 アルティヴュー， インク． 抗体ベースの交換イメージングを迅速化する組成物及び方法
WO2018022809A1 (en) * 2016-07-27 2018-02-01 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
EP3502232A4 (en) * 2016-08-22 2020-04-01 Nissan Chemical Corporation CELLULAR SCAFFOLDING MATERIAL USING A NUCLEIC ACID APTAMER BINDING E-CADHERIN
KR102606620B1 (ko) * 2016-12-09 2023-11-28 얼티뷰, 인크. 표지된 핵산 조영제를 사용한 다중 이미지형성을 위한 개선된 방법
CN117187346A (zh) 2017-03-31 2023-12-08 乌尔蒂维尤股份有限公司 Dna-抗原交换和扩增
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
US11935240B2 (en) 2017-12-08 2024-03-19 Ultivue, Inc. Validation methods for multiplexed imaging method
US10871485B2 (en) 2018-04-13 2020-12-22 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
US20210223254A1 (en) * 2018-07-09 2021-07-22 Ultivue, Inc. Methods for Multicolor Multiplex Imaging
CN109628556A (zh) * 2018-11-27 2019-04-16 山东师范大学 基于自催化复制介导的循环信号放大检测人8-羟基鸟嘌呤dna糖基化酶活性的方法
WO2020123742A1 (en) 2018-12-13 2020-06-18 President And Fellows Of Harvard College Amplification methods and systems for merfish and other applications
CN113166799A (zh) 2018-12-14 2021-07-23 乌尔蒂维尤股份有限公司 用于依次检测靶标的方法和组合物
WO2020139757A1 (en) * 2018-12-27 2020-07-02 Bio-Rad Laboratories, Inc. Sequential multiplex western blotting
JP2022536397A (ja) * 2019-06-14 2022-08-15 アコヤ・バイオサイエンシズ・インコーポレイテッド 多重組織撮像
CN111004622B (zh) * 2019-11-28 2023-08-15 郑州轻工业大学 一种检测多巴胺的高灵敏荧光探针的制备方法及其应用
US20230221326A1 (en) 2019-12-30 2023-07-13 Ultivue, Inc. Methods for Reducing Nonspecific Interactions on Biological Samples
US20220064698A1 (en) * 2020-08-26 2022-03-03 Akoya Biosciences, Inc. Multiplexed imaging reagent compositions and kits
CN112014563B (zh) * 2020-08-27 2022-02-15 武汉大学 直接检测血液中循环肿瘤细胞的分子信标传递纳米探针、其制备方法及应用
CA3192943A1 (en) * 2020-09-16 2022-03-24 Nanostring Technologies, Inc. Chemical compositions and methods of using the same
GB2603935B (en) 2021-02-19 2023-11-01 Micrographia Bio Ltd Method and kits for multiplex fluorescent microscopy
US20240255497A1 (en) * 2021-05-19 2024-08-01 Leica Microsystems Cms Gmbh Method for analyzing a biological sample or a chemical compound or a chemical element
CN113368899B (zh) * 2021-07-03 2022-05-10 太原理工大学 一种高酸密度拟纤维素酶树脂固体酸催化剂的制备方法
CN113584133B (zh) * 2021-08-30 2024-03-26 中国药科大学 基于颜色编码与可编程性荧光探针的多重靶标原位检测方法
US20230408382A1 (en) 2022-05-31 2023-12-21 Ultivue, Inc. Methods and Systems for Removing a Histological Stain From a Sample
GB202212055D0 (en) 2022-08-18 2022-10-05 Micrographia Bio Ltd Method and kits
EP4662234A2 (en) * 2023-02-08 2025-12-17 Toreador Therapeutics, Inc. Super-resolution microscopy (srm) multiplexing with cleavable moieties
EP4450971A1 (en) * 2023-04-21 2024-10-23 Leica Microsystems CMS GmbH Marker for analysing a biological sample
CN116686824A (zh) * 2023-06-07 2023-09-05 晶准生物医学（深圳）有限公司 血液样本保存液及其制备方法和应用
CN116990271B (zh) * 2023-06-29 2025-09-16 四川大学 基于比率荧光的四氢大麻酚及其代谢物的可视化检验方法
CN116794290B (zh) * 2023-07-03 2025-04-08 上海市第十人民医院 一种基于可逆dna自组装的组织切片靶标蛋白多重分析方法
CN117347621B (zh) * 2023-08-25 2024-03-12 广东省农业科学院农业生物基因研究中心 一种用蛋白模拟抗原-纳米抗体检测黄曲霉毒素b1的方法
CN120334329B (zh) * 2025-06-18 2025-08-22 徐州市中心医院 一种雌二醇生物传感器及其构建方法和应用

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4421891C2 (de) 1994-06-23 1996-05-15 Helmut Prof Dr Med Feucht Verfahren zum Nachweis von komplementaktivierenden IgG-Antikörpern gegen HLA-DR-Antigene
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE19709348C2 (de) 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
DE19717904A1 (de) 1997-04-23 1998-10-29 Diagnostikforschung Inst Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie
US20100081134A1 (en) 1997-07-21 2010-04-01 Mirkin Chad A Bio-barcode based detection of target analytes
US6083486A (en) 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
JP2002520448A (ja) 1998-07-10 2002-07-09 クロマジェン インコーポレーティッド 加水分解酵素の新規蛍光原基質
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
AU3567900A (en) 1999-03-30 2000-10-16 Solexa Ltd. Polynucleotide sequencing
US6942968B1 (en) 1999-08-30 2005-09-13 Illumina, Inc. Array compositions for improved signal detection
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
US7534568B2 (en) * 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
DE10014685B4 (de) 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
US20020142310A1 (en) 2000-04-07 2002-10-03 Leif Andersson Variants of the human AMP-activated protein kinase gamma 3 subunit
US20020106648A1 (en) 2000-05-05 2002-08-08 Lizardi Paul M. Highly multiplexed reporter carrier systems
JP2003535599A (ja) 2000-06-06 2003-12-02 ティーエム バイオサイエンス コーポレイション 核酸の捕捉部とその使用
US20030044353A1 (en) 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
WO2002079771A1 (en) 2001-03-30 2002-10-10 The Samuel Roberts Noble Foundation, Inc. Silver destaining method
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
US20020173053A1 (en) 2001-04-27 2002-11-21 Bassam Damaj Multiple simultaneous antigen detection by immunohistochemistry
US20040241688A1 (en) 2001-07-19 2004-12-02 Cuneyt Bukusoglu Human tissue specific drug screening procedure
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
WO2003061711A2 (en) 2002-01-16 2003-07-31 Visen Medical, Inc. Chromophore probes for optical imaging
JP4061401B2 (ja) 2002-03-07 2008-03-19 国立大学法人九州大学 Ｄｎａの自己組織化によるｄｎａナノケージ及びその製造方法、並びにそれを用いたｄｎａナノチューブ、分子キャリアー
WO2003100101A1 (en) 2002-05-28 2003-12-04 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
WO2004061446A1 (en) 2002-12-18 2004-07-22 Aclara Biosciences, Inc. Single cell analysis of membrane molecules
US20040241776A1 (en) 2003-05-22 2004-12-02 Agdia, Inc. Multiplex enzyme-linked immunosorbent assay for detecting multiple analytes
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20050074781A1 (en) 2003-10-02 2005-04-07 Herbert von Schroeder Nucleic acid braided J-probes
WO2006012302A2 (en) 2004-06-28 2006-02-02 Exagen Diagnostics, Inc. Methods for rna fluorescence in situ hybridization
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
EP2312317A3 (en) 2005-05-03 2011-06-08 Life Technologies Corporation Fluorescent detection system and dye set for use therewith
AU2006341607B2 (en) 2005-05-31 2011-03-17 Applied Biosystems, Llc. Multiplexed amplification of short nucleic acids
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US20060292616A1 (en) 2005-06-23 2006-12-28 U.S. Genomics, Inc. Single molecule miRNA-based disease diagnostic methods
US7494776B2 (en) 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
AU2006330830B2 (en) 2005-12-23 2013-01-10 Bruker Spatial Biology, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
US7838302B2 (en) 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
US7741045B2 (en) * 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US8305579B2 (en) 2006-11-16 2012-11-06 Thomas Pirrie Treynor Sequential analysis of biological samples
US20100216978A1 (en) 2007-04-17 2010-08-26 Dsna-Farber Cancer Institute Inc. Wireframe nanostructures
US20080287668A1 (en) 2007-05-14 2008-11-20 Tihamer Thomas Toth-Fejel Nanostructures and methods of making
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
EP2216338B1 (en) 2007-11-19 2013-03-20 Japan Advanced Institute of Science and Technology Light-responsive artificial nucleotide having photo-crosslinking ability
US8564792B2 (en) 2007-12-21 2013-10-22 President And Fellows Of Harvard College Sub-diffraction limit image resolution in three dimensions
US9340416B2 (en) 2008-08-13 2016-05-17 California Institute Of Technology Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems
US9677125B2 (en) * 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
US9315860B2 (en) * 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
WO2012051386A2 (en) 2010-10-14 2012-04-19 Meso Scale Technologies, Llc Reagent storage in an assay device
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
US20130184184A1 (en) 2010-11-22 2013-07-18 The University Of Chicago Methods and/or Use of Oligonucleotide Conjugates Having Varied Degrees of Labeling for Assays and Detections
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
AU2013202354A1 (en) 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
US10150988B2 (en) * 2012-08-13 2018-12-11 William Marsh Rice University Multiplexed in situ molecular analyses and programmable molecular probes for regulated single amplification
AU2013337280B2 (en) 2012-11-05 2018-11-08 Takara Bio Usa, Inc. Barcoding nucleic acids
AU2014219180A1 (en) 2013-02-20 2015-10-08 Emory University Methods of sequencing nucleic acids in mixtures and compositions related thereto
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
CA2938082A1 (en) 2013-12-15 2015-06-18 President And Fellows Of Harvard College Methods and compositions relating to optical super-resolution patterning
US10294510B2 (en) 2014-03-11 2019-05-21 President And Fellows Of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jungmann 등, Nature methods. Vol.11, No. 3, 페이지 313-318 (2014.02.02.)*

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