JP6757255B2 - プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング - Google Patents

プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング Download PDF

Info

Publication number
JP6757255B2
JP6757255B2 JP2016551812A JP2016551812A JP6757255B2 JP 6757255 B2 JP6757255 B2 JP 6757255B2 JP 2016551812 A JP2016551812 A JP 2016551812A JP 2016551812 A JP2016551812 A JP 2016551812A JP 6757255 B2 JP6757255 B2 JP 6757255B2
Authority
JP
Japan
Prior art keywords
nucleic acid
imager
chain
docking
labeled imager
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016551812A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017509872A (ja
JP2017509872A5 (enExample
Inventor
イン，ペン
アガスティ，サリット
チェン，シ
ユングマン，ラルフ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of JP2017509872A publication Critical patent/JP2017509872A/ja
Publication of JP2017509872A5 publication Critical patent/JP2017509872A5/ja
Application granted granted Critical
Publication of JP6757255B2 publication Critical patent/JP6757255B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/601—Detection means characterised by use of a special device being a microscope, e.g. atomic force microscopy [AFM]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2016551812A 2014-03-11 2015-03-11 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング Active JP6757255B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461951461P 2014-03-11 2014-03-11
US61/951,461 2014-03-11
PCT/US2015/020034 WO2015138653A1 (en) 2014-03-11 2015-03-11 High-throughput and highly multiplexed imaging with programmable nucleic acid probes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020144012A Division JP7264856B2 (ja) 2014-03-11 2020-08-28 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング

Publications (3)

Publication Number Publication Date
JP2017509872A JP2017509872A (ja) 2017-04-06
JP2017509872A5 JP2017509872A5 (enExample) 2018-04-19
JP6757255B2 true JP6757255B2 (ja) 2020-09-16

Family

ID=54072381

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2016551812A Active JP6757255B2 (ja) 2014-03-11 2015-03-11 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング
JP2020144012A Active JP7264856B2 (ja) 2014-03-11 2020-08-28 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング
JP2023027120A Active JP7520172B2 (ja) 2014-03-11 2023-02-24 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2020144012A Active JP7264856B2 (ja) 2014-03-11 2020-08-28 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング
JP2023027120A Active JP7520172B2 (ja) 2014-03-11 2023-02-24 プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング

Country Status (11)

Country Link
US (5) US10294510B2 (enExample)
EP (4) EP3117214B1 (enExample)
JP (3) JP6757255B2 (enExample)
KR (2) KR102313982B1 (enExample)
CN (2) CN110004208A (enExample)
AU (2) AU2015229406C1 (enExample)
BR (1) BR112016018314A2 (enExample)
CA (1) CA2932943C (enExample)
IL (1) IL246962B (enExample)
RU (1) RU2662969C2 (enExample)
WO (1) WO2015138653A1 (enExample)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
HK1221747A1 (zh) 2013-07-30 2017-06-09 哈佛学院院长及董事 基於dna的定量成像和超分辨成像
JP6757255B2 (ja) 2014-03-11 2020-09-16 プレジデント アンド フェローズ オブ ハーバード カレッジ プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
ES3014093T3 (en) 2014-07-30 2025-04-16 Harvard College Method for determining nucleic acids
KR102557419B1 (ko) 2015-08-07 2023-07-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 단백질-단백질 상호작용의 초해상도 영상화
AU2017257597A1 (en) 2016-04-26 2018-10-04 Ultivue, Inc. Super-resolution immunofluorescence with diffraction-limited preview
WO2017197147A2 (en) * 2016-05-11 2017-11-16 Atkinson Robert G Molecular beacon comprising prefabricated components and associated products, processes, and methods of use
EP4047368A1 (en) * 2016-05-19 2022-08-24 Toppan Printing Co., Ltd. Method and kit for target molecule detection
JP2019526034A (ja) * 2016-06-02 2019-09-12 アルティヴュー， インク． 抗体ベースの交換イメージングを迅速化する組成物及び方法
CN116064727A (zh) * 2016-07-27 2023-05-05 斯坦福大学托管董事会 高度复用荧光成像
JPWO2018038076A1 (ja) * 2016-08-22 2019-06-20 日産化学株式会社 Ｅ−カドヘリン結合型核酸アプタマーを用いる細胞足場材料
AU2017370751B2 (en) * 2016-12-09 2023-11-09 Ultivue, Inc. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
WO2018183876A1 (en) * 2017-03-31 2018-10-04 Ultivue, Inc. Dna-antigen exchange and amplification
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
US11935240B2 (en) 2017-12-08 2024-03-19 Ultivue, Inc. Validation methods for multiplexed imaging method
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
EP3821251A4 (en) * 2018-07-09 2022-08-24 Ultivue, Inc. MULTIPLEX MULTICOLOR IMAGING PROCESSES
CN109628556A (zh) * 2018-11-27 2019-04-16 山东师范大学 基于自催化复制介导的循环信号放大检测人8-羟基鸟嘌呤dna糖基化酶活性的方法
CN113366116A (zh) 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统
KR20210104030A (ko) 2018-12-14 2021-08-24 얼티뷰, 인크. 표적을 순차적으로 검출하는 방법 및 조성물
EP3903104A4 (en) * 2018-12-27 2022-09-21 Bio-Rad Laboratories, Inc. SEQUENCE MULTIPLEX WESTERN BLOT
WO2020252488A2 (en) * 2019-06-14 2020-12-17 Akoya Biosciences, Inc. Multiplexed tissue imaging
CN111004622B (zh) * 2019-11-28 2023-08-15 郑州轻工业大学 一种检测多巴胺的高灵敏荧光探针的制备方法及其应用
US20230221326A1 (en) 2019-12-30 2023-07-13 Ultivue, Inc. Methods for Reducing Nonspecific Interactions on Biological Samples
US20220064698A1 (en) * 2020-08-26 2022-03-03 Akoya Biosciences, Inc. Multiplexed imaging reagent compositions and kits
CN112014563B (zh) * 2020-08-27 2022-02-15 武汉大学 直接检测血液中循环肿瘤细胞的分子信标传递纳米探针、其制备方法及应用
AU2021343471A1 (en) * 2020-09-16 2023-05-04 Bruker Spatial Biology, Inc. Chemical compositions and methods of using the same
GB2603935B (en) 2021-02-19 2023-11-01 Micrographia Bio Ltd Method and kits for multiplex fluorescent microscopy
JP2024521682A (ja) * 2021-05-19 2024-06-04 ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング 生体試料または化合物または化学元素を分析するための方法
CN113368899B (zh) * 2021-07-03 2022-05-10 太原理工大学 一种高酸密度拟纤维素酶树脂固体酸催化剂的制备方法
CN113584133B (zh) * 2021-08-30 2024-03-26 中国药科大学 基于颜色编码与可编程性荧光探针的多重靶标原位检测方法
US20230408382A1 (en) 2022-05-31 2023-12-21 Ultivue, Inc. Methods and Systems for Removing a Histological Stain From a Sample
GB202212055D0 (en) 2022-08-18 2022-10-05 Micrographia Bio Ltd Method and kits
EP4662234A2 (en) * 2023-02-08 2025-12-17 Toreador Therapeutics, Inc. Super-resolution microscopy (srm) multiplexing with cleavable moieties
EP4450971A1 (en) * 2023-04-21 2024-10-23 Leica Microsystems CMS GmbH Marker for analysing a biological sample
CN116686824A (zh) * 2023-06-07 2023-09-05 晶准生物医学（深圳）有限公司 血液样本保存液及其制备方法和应用
CN116990271B (zh) * 2023-06-29 2025-09-16 四川大学 基于比率荧光的四氢大麻酚及其代谢物的可视化检验方法
CN116794290B (zh) * 2023-07-03 2025-04-08 上海市第十人民医院 一种基于可逆dna自组装的组织切片靶标蛋白多重分析方法
CN117347621B (zh) * 2023-08-25 2024-03-12 广东省农业科学院农业生物基因研究中心 一种用蛋白模拟抗原-纳米抗体检测黄曲霉毒素b1的方法
CN120334329B (zh) * 2025-06-18 2025-08-22 徐州市中心医院 一种雌二醇生物传感器及其构建方法和应用

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4421891C2 (de) 1994-06-23 1996-05-15 Helmut Prof Dr Med Feucht Verfahren zum Nachweis von komplementaktivierenden IgG-Antikörpern gegen HLA-DR-Antigene
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE19709348C2 (de) 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
DE19717904A1 (de) 1997-04-23 1998-10-29 Diagnostikforschung Inst Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie
US20100081134A1 (en) 1997-07-21 2010-04-01 Mirkin Chad A Bio-barcode based detection of target analytes
US6083486A (en) 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
US6635435B1 (en) 1998-07-10 2003-10-21 Chromagen, Inc. Fluorogenic substrates and their use
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
AU2246601A (en) 1999-08-30 2001-04-10 Illumina, Inc. Methods for improving signal detection from an array
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
US7534568B2 (en) * 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
DE10014685B4 (de) 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
US20020142310A1 (en) 2000-04-07 2002-10-03 Leif Andersson Variants of the human AMP-activated protein kinase gamma 3 subunit
WO2001086296A2 (en) 2000-05-05 2001-11-15 Agilix Corporation Highly multiplexed reporter carrier systems
EP1313881A2 (en) 2000-06-06 2003-05-28 TM Bioscience Corporation Capture moieties for nucleic acids and uses thereof
US20030044353A1 (en) 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US20050230255A1 (en) 2001-03-30 2005-10-20 Sumner Lloyd W Silver destaining method
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
US20020173053A1 (en) 2001-04-27 2002-11-21 Bassam Damaj Multiple simultaneous antigen detection by immunohistochemistry
AU2002319613A1 (en) 2001-07-19 2003-03-03 Signet Laboratories, Inc. Human tissue specific drug screening procedure
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
WO2003061711A2 (en) 2002-01-16 2003-07-31 Visen Medical, Inc. Chromophore probes for optical imaging
JP4061401B2 (ja) 2002-03-07 2008-03-19 国立大学法人九州大学 Ｄｎａの自己組織化によるｄｎａナノケージ及びその製造方法、並びにそれを用いたｄｎａナノチューブ、分子キャリアー
EP1546380A4 (en) 2002-05-28 2007-02-14 Us Genomics Inc METHODS AND APPARATUSES FOR ANALYZING SIMPLE POLYMERS
AU2003287527A1 (en) 2002-12-18 2004-07-29 Aclara Biosciences, Inc. Single cell analysis of membrane molecules
WO2005017485A2 (en) 2003-05-22 2005-02-24 Agdia, Inc. Multiplex enzyme-linked immunosorbent assay for detecting multiple analytes
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20050074781A1 (en) 2003-10-02 2005-04-07 Herbert von Schroeder Nucleic acid braided J-probes
US20050287578A1 (en) 2004-06-28 2005-12-29 Exagen Diagnostics, Inc. Methods for RNA fluorescence in situ hybridization
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
EP1886145A2 (en) 2005-05-03 2008-02-13 Applera Corporation Fluorescent detection system and dye set for use therewith
US20070077570A1 (en) 2005-05-31 2007-04-05 Applera Corporation Multiplexed amplification of short nucleic acids
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US20060292616A1 (en) 2005-06-23 2006-12-28 U.S. Genomics, Inc. Single molecule miRNA-based disease diagnostic methods
US7494776B2 (en) * 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
EP1963531B1 (en) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
US7838302B2 (en) 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
US8305579B2 (en) 2006-11-16 2012-11-06 Thomas Pirrie Treynor Sequential analysis of biological samples
US7741045B2 (en) * 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
WO2008130585A2 (en) 2007-04-17 2008-10-30 Dana-Farber Cancer Institute Wireframe nanostructures
US20080287668A1 (en) 2007-05-14 2008-11-20 Tihamer Thomas Toth-Fejel Nanostructures and methods of making
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
JP4940311B2 (ja) 2007-11-19 2012-05-30 国立大学法人北陸先端科学技術大学院大学 光クロスリンク能を有する光応答性人工ヌクレオチド
CN101918816B (zh) 2007-12-21 2015-12-02 哈佛大学 三维中的亚衍射极限图像分辨率
US9340416B2 (en) 2008-08-13 2016-05-17 California Institute Of Technology Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems
US9677125B2 (en) * 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
US9315860B2 (en) * 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
CA3139049C (en) * 2010-10-14 2024-10-15 Meso Scale Technologies Llc Reagent storage in an assay device
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
US20120258881A1 (en) 2010-11-22 2012-10-11 The University Of Chicago Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
AU2013202354A1 (en) 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
WO2014028538A2 (en) * 2012-08-13 2014-02-20 William Marsh Rice University Multiplexed in situ molecular analyses and programmable molecular probes for regulated signal amplification
CA2889862C (en) 2012-11-05 2021-02-16 Rubicon Genomics, Inc. Barcoding nucleic acids
ES2900102T3 (es) 2013-02-20 2022-03-15 Univ Emory Composiciones para la secuenciación de ácidos nucleicos en mezclas
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
WO2015089506A2 (en) 2013-12-15 2015-06-18 President And Fellows Of Harvard College Methods and compositions relating to optical super-resolution patterning
JP6757255B2 (ja) 2014-03-11 2020-09-16 プレジデント アンド フェローズ オブ ハーバード カレッジ プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング

Also Published As

Publication number Publication date
EP4245859A3 (en) 2023-11-08
KR102313982B1 (ko) 2021-10-18
IL246962A0 (en) 2016-09-29
US10190151B2 (en) 2019-01-29
JP2017509872A (ja) 2017-04-06
KR102382892B1 (ko) 2022-04-08
AU2015229406C1 (en) 2021-01-07
BR112016018314A2 (pt) 2017-10-17
KR20210127799A (ko) 2021-10-22
AU2020239730A1 (en) 2020-10-15
US10294510B2 (en) 2019-05-21
US20170137864A1 (en) 2017-05-18
JP2023067901A (ja) 2023-05-16
CN105980855A (zh) 2016-09-28
EP3117214A1 (en) 2017-01-18
EP4030166A1 (en) 2022-07-20
CN105980855B (zh) 2019-04-09
IL246962B (en) 2020-10-29
WO2015138653A1 (en) 2015-09-17
AU2020239730B2 (en) 2024-01-18
RU2662969C2 (ru) 2018-07-31
NZ720849A (en) 2024-04-26
CA2932943A1 (en) 2015-09-17
EP3117214A4 (en) 2017-12-20
EP3686599B1 (en) 2022-01-19
US9944972B2 (en) 2018-04-17
JP7264856B2 (ja) 2023-04-25
EP3117214B1 (en) 2020-05-06
AU2015229406A1 (en) 2016-06-23
EP3686599A1 (en) 2020-07-29
JP7520172B2 (ja) 2024-07-22
KR20160130993A (ko) 2016-11-15
EP4245859A2 (en) 2023-09-20
US20190323061A1 (en) 2019-10-24
US20160319328A1 (en) 2016-11-03
CA2932943C (en) 2023-09-26
AU2015229406B2 (en) 2020-07-09
CN110004208A (zh) 2019-07-12
EP4030166B1 (en) 2023-06-28
JP2020193988A (ja) 2020-12-03
US20230027974A1 (en) 2023-01-26
US20180216159A1 (en) 2018-08-02
RU2016139490A (ru) 2018-04-11

Similar Documents

Publication Publication Date Title
JP6757255B2 (ja) プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング
EP3696277B2 (en) Quantitative dna-based imaging and super-resolution imaging
HK1227106A1 (en) High-throughput and highly multiplexed imaging with programmable nucleic acid probes
HK1227106B (zh) 利用可编程核酸探针的高通量且高度多路复用的成像
HK40011429A (en) High-throughput and highly multiplexed imaging with programmable nucleic acid probes

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180309

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180309

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190131

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190305

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190604

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191129

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200110

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200630

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200722

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200828

R150 Certificate of patent or registration of utility model

Ref document number: 6757255

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250