JP2017509872A5 - - Google Patents
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- JP2017509872A5 JP2017509872A5 JP2016551812A JP2016551812A JP2017509872A5 JP 2017509872 A5 JP2017509872 A5 JP 2017509872A5 JP 2016551812 A JP2016551812 A JP 2016551812A JP 2016551812 A JP2016551812 A JP 2016551812A JP 2017509872 A5 JP2017509872 A5 JP 2017509872A5
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- Prior art keywords
- strand
- labeled imager
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- specific binding
- sample
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- 238000000034 method Methods 0.000 claims 29
- 238000003032 molecular docking Methods 0.000 claims 11
- 230000009870 specific binding Effects 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 238000003776 cleavage reaction Methods 0.000 claims 7
- 230000007017 scission Effects 0.000 claims 7
- 230000002255 enzymatic effect Effects 0.000 claims 3
- MXHRCPNRJAMMIM-SHYZEUOFSA-N 2'-deoxyuridine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-SHYZEUOFSA-N 0.000 claims 2
- 108091023037 Aptamer Proteins 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 210000001124 body fluid Anatomy 0.000 claims 2
- 239000010839 body fluid Substances 0.000 claims 2
- 230000015556 catabolic process Effects 0.000 claims 2
- 239000013592 cell lysate Substances 0.000 claims 2
- 238000001218 confocal laser scanning microscopy Methods 0.000 claims 2
- 238000001317 epifluorescence microscopy Methods 0.000 claims 2
- 239000003446 ligand Substances 0.000 claims 2
- 239000006166 lysate Substances 0.000 claims 2
- 230000004048 modification Effects 0.000 claims 2
- 238000012986 modification Methods 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 150000003384 small molecules Chemical class 0.000 claims 2
- 102000006382 Ribonucleases Human genes 0.000 claims 1
- 108010083644 Ribonucleases Proteins 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- 238000002144 chemical decomposition reaction Methods 0.000 claims 1
- 238000007385 chemical modification Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 238000003384 imaging method Methods 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461951461P | 2014-03-11 | 2014-03-11 | |
| US61/951,461 | 2014-03-11 | ||
| PCT/US2015/020034 WO2015138653A1 (en) | 2014-03-11 | 2015-03-11 | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020144012A Division JP7264856B2 (ja) | 2014-03-11 | 2020-08-28 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017509872A JP2017509872A (ja) | 2017-04-06 |
| JP2017509872A5 true JP2017509872A5 (enExample) | 2018-04-19 |
| JP6757255B2 JP6757255B2 (ja) | 2020-09-16 |
Family
ID=54072381
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016551812A Active JP6757255B2 (ja) | 2014-03-11 | 2015-03-11 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| JP2020144012A Active JP7264856B2 (ja) | 2014-03-11 | 2020-08-28 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| JP2023027120A Active JP7520172B2 (ja) | 2014-03-11 | 2023-02-24 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020144012A Active JP7264856B2 (ja) | 2014-03-11 | 2020-08-28 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| JP2023027120A Active JP7520172B2 (ja) | 2014-03-11 | 2023-02-24 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US10294510B2 (enExample) |
| EP (4) | EP4030166B1 (enExample) |
| JP (3) | JP6757255B2 (enExample) |
| KR (2) | KR102313982B1 (enExample) |
| CN (2) | CN105980855B (enExample) |
| AU (2) | AU2015229406C1 (enExample) |
| BR (1) | BR112016018314A2 (enExample) |
| CA (1) | CA2932943C (enExample) |
| IL (1) | IL246962B (enExample) |
| RU (1) | RU2662969C2 (enExample) |
| WO (1) | WO2015138653A1 (enExample) |
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| EP4030166B1 (en) | 2014-03-11 | 2023-06-28 | President and Fellows of Harvard College | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
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| JP2024521683A (ja) * | 2021-05-19 | 2024-06-04 | ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 生体試料を分析するためのマーカー、方法および装置 |
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| CN113368899B (zh) * | 2021-07-03 | 2022-05-10 | 太原理工大学 | 一种高酸密度拟纤维素酶树脂固体酸催化剂的制备方法 |
| CN113584133B (zh) * | 2021-08-30 | 2024-03-26 | 中国药科大学 | 基于颜色编码与可编程性荧光探针的多重靶标原位检测方法 |
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-
2015
- 2015-03-11 EP EP22151320.3A patent/EP4030166B1/en active Active
- 2015-03-11 BR BR112016018314A patent/BR112016018314A2/pt not_active Application Discontinuation
- 2015-03-11 AU AU2015229406A patent/AU2015229406C1/en active Active
- 2015-03-11 KR KR1020167021588A patent/KR102313982B1/ko active Active
- 2015-03-11 JP JP2016551812A patent/JP6757255B2/ja active Active
- 2015-03-11 EP EP15762370.3A patent/EP3117214B1/en active Active
- 2015-03-11 KR KR1020217032767A patent/KR102382892B1/ko active Active
- 2015-03-11 RU RU2016139490A patent/RU2662969C2/ru active
- 2015-03-11 EP EP23176015.8A patent/EP4245859A3/en active Pending
- 2015-03-11 CA CA2932943A patent/CA2932943C/en active Active
- 2015-03-11 CN CN201580006969.2A patent/CN105980855B/zh active Active
- 2015-03-11 CN CN201910186444.1A patent/CN110004208A/zh active Pending
- 2015-03-11 US US15/108,911 patent/US10294510B2/en active Active
- 2015-03-11 EP EP20157624.6A patent/EP3686599B1/en active Active
- 2015-03-11 WO PCT/US2015/020034 patent/WO2015138653A1/en not_active Ceased
-
2016
- 2016-07-26 IL IL246962A patent/IL246962B/en active IP Right Grant
-
2017
- 2017-01-19 US US15/410,700 patent/US9944972B2/en active Active
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2018
- 2018-03-23 US US15/934,031 patent/US10190151B2/en active Active
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2019
- 2019-03-18 US US16/357,099 patent/US20190323061A1/en not_active Abandoned
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2020
- 2020-08-28 JP JP2020144012A patent/JP7264856B2/ja active Active
- 2020-09-24 AU AU2020239730A patent/AU2020239730B2/en active Active
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2022
- 2022-07-08 US US17/811,486 patent/US20230027974A1/en active Pending
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2023
- 2023-02-24 JP JP2023027120A patent/JP7520172B2/ja active Active
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