JP2017509872A5 - - Google Patents
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- JP2017509872A5 JP2017509872A5 JP2016551812A JP2016551812A JP2017509872A5 JP 2017509872 A5 JP2017509872 A5 JP 2017509872A5 JP 2016551812 A JP2016551812 A JP 2016551812A JP 2016551812 A JP2016551812 A JP 2016551812A JP 2017509872 A5 JP2017509872 A5 JP 2017509872A5
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- JP
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- Prior art keywords
- strand
- labeled imager
- labeled
- specific binding
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 29
- 238000003032 molecular docking Methods 0.000 claims 11
- 230000009870 specific binding Effects 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 238000003776 cleavage reaction Methods 0.000 claims 7
- 230000007017 scission Effects 0.000 claims 7
- 230000002255 enzymatic effect Effects 0.000 claims 3
- MXHRCPNRJAMMIM-SHYZEUOFSA-N 2'-deoxyuridine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-SHYZEUOFSA-N 0.000 claims 2
- 108091023037 Aptamer Proteins 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 210000001124 body fluid Anatomy 0.000 claims 2
- 239000010839 body fluid Substances 0.000 claims 2
- 230000015556 catabolic process Effects 0.000 claims 2
- 239000013592 cell lysate Substances 0.000 claims 2
- 238000001218 confocal laser scanning microscopy Methods 0.000 claims 2
- 238000001317 epifluorescence microscopy Methods 0.000 claims 2
- 239000003446 ligand Substances 0.000 claims 2
- 239000006166 lysate Substances 0.000 claims 2
- 230000004048 modification Effects 0.000 claims 2
- 238000012986 modification Methods 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 150000003384 small molecules Chemical class 0.000 claims 2
- 102000006382 Ribonucleases Human genes 0.000 claims 1
- 108010083644 Ribonucleases Proteins 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- 238000002144 chemical decomposition reaction Methods 0.000 claims 1
- 238000007385 chemical modification Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 238000003384 imaging method Methods 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461951461P | 2014-03-11 | 2014-03-11 | |
| US61/951,461 | 2014-03-11 | ||
| PCT/US2015/020034 WO2015138653A1 (en) | 2014-03-11 | 2015-03-11 | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020144012A Division JP7264856B2 (ja) | 2014-03-11 | 2020-08-28 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017509872A JP2017509872A (ja) | 2017-04-06 |
| JP2017509872A5 true JP2017509872A5 (enExample) | 2018-04-19 |
| JP6757255B2 JP6757255B2 (ja) | 2020-09-16 |
Family
ID=54072381
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016551812A Active JP6757255B2 (ja) | 2014-03-11 | 2015-03-11 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| JP2020144012A Active JP7264856B2 (ja) | 2014-03-11 | 2020-08-28 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| JP2023027120A Active JP7520172B2 (ja) | 2014-03-11 | 2023-02-24 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020144012A Active JP7264856B2 (ja) | 2014-03-11 | 2020-08-28 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| JP2023027120A Active JP7520172B2 (ja) | 2014-03-11 | 2023-02-24 | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US10294510B2 (enExample) |
| EP (4) | EP3686599B1 (enExample) |
| JP (3) | JP6757255B2 (enExample) |
| KR (2) | KR102382892B1 (enExample) |
| CN (2) | CN110004208A (enExample) |
| AU (2) | AU2015229406C1 (enExample) |
| BR (1) | BR112016018314A2 (enExample) |
| CA (1) | CA2932943C (enExample) |
| IL (1) | IL246962B (enExample) |
| RU (1) | RU2662969C2 (enExample) |
| WO (1) | WO2015138653A1 (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| EP3027773B1 (en) | 2013-07-30 | 2020-03-25 | President and Fellows of Harvard College | Quantitative dna-based imaging and super-resolution imaging |
| US10294510B2 (en) | 2014-03-11 | 2019-05-21 | President And Fellows Of Harvard College | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| CN107075545A (zh) | 2014-07-30 | 2017-08-18 | 哈佛学院院长及董事 | 探针文库构建 |
| US11092606B2 (en) | 2015-08-07 | 2021-08-17 | President And Fellows Of Harvard College | Super resolution imaging of protein-protein interactions |
| AU2017257597A1 (en) * | 2016-04-26 | 2018-10-04 | Ultivue, Inc. | Super-resolution immunofluorescence with diffraction-limited preview |
| WO2017197147A2 (en) * | 2016-05-11 | 2017-11-16 | Atkinson Robert G | Molecular beacon comprising prefabricated components and associated products, processes, and methods of use |
| CN109477834A (zh) * | 2016-05-19 | 2019-03-15 | 凸版印刷株式会社 | 目标分子的检测方法及目标分子检测试剂盒 |
| CN109891218A (zh) * | 2016-06-02 | 2019-06-14 | 乌尔蒂维尤股份有限公司 | 促进基于抗体的交换成像的组合物和方法 |
| US10370698B2 (en) * | 2016-07-27 | 2019-08-06 | The Board Of Trustees Of The Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| TW201821609A (zh) * | 2016-08-22 | 2018-06-16 | Nissan Chemical Ind Ltd | 使用ｅ－鈣黏蛋白結合型核酸適體之細胞貼附材料 |
| EP3551765B1 (en) * | 2016-12-09 | 2026-02-04 | Ultivue, Inc. | Improved methods for multiplex imaging using labeled nucleic acid imaging agents |
| EP3601339A4 (en) | 2017-03-31 | 2020-11-04 | Ultivue, Inc. | DNA ANTIGEN EXCHANGE AND AMPLIFICATION |
| WO2018218150A1 (en) | 2017-05-26 | 2018-11-29 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
| US11935240B2 (en) | 2017-12-08 | 2024-03-19 | Ultivue, Inc. | Validation methods for multiplexed imaging method |
| WO2019200326A1 (en) | 2018-04-13 | 2019-10-17 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| AT521238B1 (de) | 2018-05-09 | 2020-02-15 | Lifetaq Analytics Gmbh | In-situ zellanalyse im zellkultursystem |
| WO2020014036A1 (en) * | 2018-07-09 | 2020-01-16 | Ultivue, Inc. | Methods for multicolor multiplex imaging |
| CN109628556A (zh) * | 2018-11-27 | 2019-04-16 | 山东师范大学 | 基于自催化复制介导的循环信号放大检测人8-羟基鸟嘌呤dna糖基化酶活性的方法 |
| CA3122569A1 (en) | 2018-12-13 | 2020-06-18 | President And Fellows Of Harvard College | Amplification methods and systems for merfish and other applications |
| US11674178B2 (en) | 2018-12-14 | 2023-06-13 | Ultivue, Inc. | Methods and compositions for sequentially detecting targets |
| US20200209229A1 (en) * | 2018-12-27 | 2020-07-02 | Bio-Rad Laboratories, Inc. | Sequential multiplex western blotting |
| WO2020252488A2 (en) * | 2019-06-14 | 2020-12-17 | Akoya Biosciences, Inc. | Multiplexed tissue imaging |
| CN111004622B (zh) * | 2019-11-28 | 2023-08-15 | 郑州轻工业大学 | 一种检测多巴胺的高灵敏荧光探针的制备方法及其应用 |
| WO2021138312A1 (en) | 2019-12-30 | 2021-07-08 | Ultivue, Inc. | Methods for reducing nonspecific interactions on biological samples |
| US20220064698A1 (en) * | 2020-08-26 | 2022-03-03 | Akoya Biosciences, Inc. | Multiplexed imaging reagent compositions and kits |
| CN112014563B (zh) * | 2020-08-27 | 2022-02-15 | 武汉大学 | 直接检测血液中循环肿瘤细胞的分子信标传递纳米探针、其制备方法及应用 |
| JP7844447B2 (ja) * | 2020-09-16 | 2026-04-13 | ブルカー スペーシャル バイオロジー，インコーポレイティド | 化学組成物およびその使用方法 |
| GB2603935B (en) | 2021-02-19 | 2023-11-01 | Micrographia Bio Ltd | Method and kits for multiplex fluorescent microscopy |
| EP4341671A1 (en) * | 2021-05-19 | 2024-03-27 | Leica Microsystems CMS GmbH | Method for analyzing a biological sample or a chemical compound or a chemical element |
| JP2024521683A (ja) * | 2021-05-19 | 2024-06-04 | ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 生体試料を分析するためのマーカー、方法および装置 |
| JP2024521684A (ja) * | 2021-05-19 | 2024-06-04 | ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 生体試料を分析するためのマーカー、方法および装置 |
| CN113368899B (zh) * | 2021-07-03 | 2022-05-10 | 太原理工大学 | 一种高酸密度拟纤维素酶树脂固体酸催化剂的制备方法 |
| CN113584133B (zh) * | 2021-08-30 | 2024-03-26 | 中国药科大学 | 基于颜色编码与可编程性荧光探针的多重靶标原位检测方法 |
| EP4533057A1 (en) | 2022-05-31 | 2025-04-09 | Ultivue, Inc. | Methods and systems for removing a histological stain from a sample |
| GB202212055D0 (en) | 2022-08-18 | 2022-10-05 | Micrographia Bio Ltd | Method and kits |
| EP4662234A2 (en) * | 2023-02-08 | 2025-12-17 | Toreador Therapeutics, Inc. | Super-resolution microscopy (srm) multiplexing with cleavable moieties |
| EP4450971A1 (en) * | 2023-04-21 | 2024-10-23 | Leica Microsystems CMS GmbH | Marker for analysing a biological sample |
| CN116686824A (zh) * | 2023-06-07 | 2023-09-05 | 晶准生物医学（深圳）有限公司 | 血液样本保存液及其制备方法和应用 |
| CN116990271B (zh) * | 2023-06-29 | 2025-09-16 | 四川大学 | 基于比率荧光的四氢大麻酚及其代谢物的可视化检验方法 |
| CN116794290B (zh) * | 2023-07-03 | 2025-04-08 | 上海市第十人民医院 | 一种基于可逆dna自组装的组织切片靶标蛋白多重分析方法 |
| CN117347621B (zh) * | 2023-08-25 | 2024-03-12 | 广东省农业科学院农业生物基因研究中心 | 一种用蛋白模拟抗原-纳米抗体检测黄曲霉毒素b1的方法 |
| CN120334329B (zh) * | 2025-06-18 | 2025-08-22 | 徐州市中心医院 | 一种雌二醇生物传感器及其构建方法和应用 |
Family Cites Families (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4421891C2 (de) | 1994-06-23 | 1996-05-15 | Helmut Prof Dr Med Feucht | Verfahren zum Nachweis von komplementaktivierenden IgG-Antikörpern gegen HLA-DR-Antigene |
| US5491063A (en) * | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| DE19709348C2 (de) | 1996-05-29 | 1999-07-01 | Schubert Walter Dr Md | Automatisches Multi-Epitop-Ligand-Kartierungsverfahren |
| DE19717904A1 (de) | 1997-04-23 | 1998-10-29 | Diagnostikforschung Inst | Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie |
| US20100081134A1 (en) | 1997-07-21 | 2010-04-01 | Mirkin Chad A | Bio-barcode based detection of target analytes |
| US6083486A (en) | 1998-05-14 | 2000-07-04 | The General Hospital Corporation | Intramolecularly-quenched near infrared fluorescent probes |
| EP1095161A2 (en) | 1998-07-10 | 2001-05-02 | Chromagen, Inc. | Novel polycyclic aromatic fluorogenic substrates for hydrolases |
| US6573043B1 (en) | 1998-10-07 | 2003-06-03 | Genentech, Inc. | Tissue analysis and kits therefor |
| WO2000034527A2 (en) | 1998-12-11 | 2000-06-15 | The Regents Of The University Of California | Targeted molecular bar codes |
| AU3567900A (en) | 1999-03-30 | 2000-10-16 | Solexa Ltd. | Polynucleotide sequencing |
| EP1212599A2 (en) | 1999-08-30 | 2002-06-12 | Illumina, Inc. | Methods for improving signal detection from an array |
| WO2001023610A2 (en) | 1999-09-29 | 2001-04-05 | Solexa Ltd. | Polynucleotide sequencing |
| US7534568B2 (en) * | 1999-10-29 | 2009-05-19 | Hologic Inc. | Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe |
| GB0002389D0 (en) | 2000-02-02 | 2000-03-22 | Solexa Ltd | Molecular arrays |
| DE10014685B4 (de) | 2000-03-24 | 2004-07-01 | Schubert, Walter, Dr. | Verfahren zur Identifizierung von zellspezifischen Zielstrukturen |
| US20020142310A1 (en) | 2000-04-07 | 2002-10-03 | Leif Andersson | Variants of the human AMP-activated protein kinase gamma 3 subunit |
| WO2001086296A2 (en) | 2000-05-05 | 2001-11-15 | Agilix Corporation | Highly multiplexed reporter carrier systems |
| WO2001094625A2 (en) | 2000-06-06 | 2001-12-13 | Tm Bioscience Corporation | Capture moieties for nucleic acids and uses thereof |
| US20030044353A1 (en) | 2001-01-05 | 2003-03-06 | Ralph Weissleder | Activatable imaging probes |
| WO2002079771A1 (en) | 2001-03-30 | 2002-10-10 | The Samuel Roberts Noble Foundation, Inc. | Silver destaining method |
| US7219016B2 (en) | 2001-04-20 | 2007-05-15 | Yale University | Systems and methods for automated analysis of cells and tissues |
| US20020173053A1 (en) | 2001-04-27 | 2002-11-21 | Bassam Damaj | Multiple simultaneous antigen detection by immunohistochemistry |
| US20040241688A1 (en) | 2001-07-19 | 2004-12-02 | Cuneyt Bukusoglu | Human tissue specific drug screening procedure |
| US8323903B2 (en) | 2001-10-12 | 2012-12-04 | Life Technologies Corporation | Antibody complexes and methods for immunolabeling |
| WO2003061711A2 (en) | 2002-01-16 | 2003-07-31 | Visen Medical, Inc. | Chromophore probes for optical imaging |
| JP4061401B2 (ja) | 2002-03-07 | 2008-03-19 | 国立大学法人九州大学 | Ｄｎａの自己組織化によるｄｎａナノケージ及びその製造方法、並びにそれを用いたｄｎａナノチューブ、分子キャリアー |
| AU2003238796A1 (en) | 2002-05-28 | 2003-12-12 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
| AU2003291249A1 (en) | 2002-12-18 | 2004-07-29 | Aclara Biosciences, Inc. | Multiplexed immunohistochemical assays using molecular tags |
| US20040241776A1 (en) | 2003-05-22 | 2004-12-02 | Agdia, Inc. | Multiplex enzyme-linked immunosorbent assay for detecting multiple analytes |
| US20050064435A1 (en) | 2003-09-24 | 2005-03-24 | Xing Su | Programmable molecular barcodes |
| US20050074781A1 (en) | 2003-10-02 | 2005-04-07 | Herbert von Schroeder | Nucleic acid braided J-probes |
| WO2006012302A2 (en) | 2004-06-28 | 2006-02-02 | Exagen Diagnostics, Inc. | Methods for rna fluorescence in situ hybridization |
| US20060024678A1 (en) | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
| EP1886145A2 (en) | 2005-05-03 | 2008-02-13 | Applera Corporation | Fluorescent detection system and dye set for use therewith |
| WO2007117256A1 (en) | 2005-05-31 | 2007-10-18 | Applera Corporation | Multiplexed amplification of short nucleic acids |
| US20070048759A1 (en) | 2005-06-10 | 2007-03-01 | Dan Luo | Detection of target molecules with labeled nucleic acid detection molecules |
| US7842793B2 (en) | 2005-06-14 | 2010-11-30 | The California Institute Of Technology | Methods of making nucleic acid nanostructures |
| US20060292616A1 (en) | 2005-06-23 | 2006-12-28 | U.S. Genomics, Inc. | Single molecule miRNA-based disease diagnostic methods |
| US7494776B2 (en) | 2005-07-07 | 2009-02-24 | Beckman Coulter, Inc. | Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands |
| ATE525482T1 (de) | 2005-12-23 | 2011-10-15 | Nanostring Technologies Inc | Nanoreporter und verfahren zu deren herstellung und verwendung |
| WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| US7838302B2 (en) | 2006-08-07 | 2010-11-23 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
| US7741045B2 (en) * | 2006-11-16 | 2010-06-22 | General Electric Company | Sequential analysis of biological samples |
| US8305579B2 (en) | 2006-11-16 | 2012-11-06 | Thomas Pirrie Treynor | Sequential analysis of biological samples |
| US20100216978A1 (en) | 2007-04-17 | 2010-08-26 | Dsna-Farber Cancer Institute Inc. | Wireframe nanostructures |
| US20080287668A1 (en) | 2007-05-14 | 2008-11-20 | Tihamer Thomas Toth-Fejel | Nanostructures and methods of making |
| US9217151B2 (en) | 2007-05-16 | 2015-12-22 | California Institute Of Technology | Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways |
| WO2009066447A1 (ja) | 2007-11-19 | 2009-05-28 | Japan Advanced Institute Of Science And Technology | 光クロスリンク能を有する光応答性人工ヌクレオチド |
| CN105403545B (zh) | 2007-12-21 | 2019-05-28 | 哈佛大学 | 三维中的亚衍射极限图像分辨率 |
| US9340416B2 (en) | 2008-08-13 | 2016-05-17 | California Institute Of Technology | Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems |
| US9677125B2 (en) * | 2009-10-21 | 2017-06-13 | General Electric Company | Detection of plurality of targets in biological samples |
| EP2494073B1 (de) * | 2009-10-26 | 2017-11-29 | AGCT GmbH | Konjugate von nukleotiden und methoden zu deren anwendung |
| US9315857B2 (en) * | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US20120004132A1 (en) * | 2010-07-02 | 2012-01-05 | Affymetrix, Inc. | Detection of Nucleic Acids and Proteins |
| US8877438B2 (en) | 2010-07-20 | 2014-11-04 | California Institute Of Technology | Self-assembled polynucleotide structure |
| EP4300100A3 (en) | 2010-10-14 | 2024-09-04 | Meso Scale Technologies, LLC | Reagent storage in an assay device |
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| WO2012071428A2 (en) | 2010-11-22 | 2012-05-31 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for assays and detections |
| US20120178081A1 (en) | 2010-12-31 | 2012-07-12 | Affymetrix. Inc. | Methods of Labeling Cells, Labeled Cells, and uses Thereof |
| US8759036B2 (en) | 2011-03-21 | 2014-06-24 | Affymetrix, Inc. | Methods for synthesizing pools of probes |
| AU2013202354A1 (en) | 2012-06-18 | 2014-01-16 | Speedx Pty Ltd | Target detection and signal amplification |
| US10150988B2 (en) | 2012-08-13 | 2018-12-11 | William Marsh Rice University | Multiplexed in situ molecular analyses and programmable molecular probes for regulated single amplification |
| CN104903466B (zh) | 2012-11-05 | 2016-11-23 | 鲁比康基因组学公司 | 条形编码核酸 |
| AU2014219180A1 (en) | 2013-02-20 | 2015-10-08 | Emory University | Methods of sequencing nucleic acids in mixtures and compositions related thereto |
| BR112015021788B1 (pt) | 2013-03-08 | 2023-02-28 | Oxford Nanopore Technologies Plc | Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit |
| CA2938082A1 (en) | 2013-12-15 | 2015-06-18 | President And Fellows Of Harvard College | Methods and compositions relating to optical super-resolution patterning |
| US10294510B2 (en) | 2014-03-11 | 2019-05-21 | President And Fellows Of Harvard College | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
-
2015
- 2015-03-11 US US15/108,911 patent/US10294510B2/en active Active
- 2015-03-11 KR KR1020217032767A patent/KR102382892B1/ko active Active
- 2015-03-11 RU RU2016139490A patent/RU2662969C2/ru active
- 2015-03-11 CN CN201910186444.1A patent/CN110004208A/zh active Pending
- 2015-03-11 CA CA2932943A patent/CA2932943C/en active Active
- 2015-03-11 EP EP20157624.6A patent/EP3686599B1/en active Active
- 2015-03-11 BR BR112016018314A patent/BR112016018314A2/pt not_active Application Discontinuation
- 2015-03-11 EP EP22151320.3A patent/EP4030166B1/en active Active
- 2015-03-11 EP EP23176015.8A patent/EP4245859A3/en active Pending
- 2015-03-11 EP EP15762370.3A patent/EP3117214B1/en active Active
- 2015-03-11 KR KR1020167021588A patent/KR102313982B1/ko active Active
- 2015-03-11 CN CN201580006969.2A patent/CN105980855B/zh active Active
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- 2015-03-11 WO PCT/US2015/020034 patent/WO2015138653A1/en not_active Ceased
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2016
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