KR102272851B1 - 항체를 정제하기 위한 연속 다단계 공정 - Google Patents

항체를 정제하기 위한 연속 다단계 공정 Download PDF

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KR102272851B1
KR102272851B1 KR1020157032904A KR20157032904A KR102272851B1 KR 102272851 B1 KR102272851 B1 KR 102272851B1 KR 1020157032904 A KR1020157032904 A KR 1020157032904A KR 20157032904 A KR20157032904 A KR 20157032904A KR 102272851 B1 KR102272851 B1 KR 102272851B1
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chromatography
chromatography column
protein
passing
buffer
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KR20160005047A (ko
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디디에 두테
셀린느 에메
로레 랑드리끄-부르탱
베누아 모테
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사노피
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Water Supply & Treatment (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
KR1020157032904A 2013-05-06 2014-05-06 항체를 정제하기 위한 연속 다단계 공정 Active KR102272851B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13305593.9 2013-05-06
EP13305593 2013-05-06
PCT/EP2014/059246 WO2014180852A1 (en) 2013-05-06 2014-05-06 Continuous multistep process for purifying antibodies

Publications (2)

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KR20160005047A KR20160005047A (ko) 2016-01-13
KR102272851B1 true KR102272851B1 (ko) 2021-07-02

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US (1) US10793622B2 (enExample)
EP (2) EP2994485B1 (enExample)
JP (2) JP6463734B2 (enExample)
KR (1) KR102272851B1 (enExample)
CN (2) CN116003579A (enExample)
AU (1) AU2014264697B2 (enExample)
BR (1) BR112015027812B1 (enExample)
CA (1) CA2911462C (enExample)
CY (1) CY1125281T1 (enExample)
DK (2) DK2994485T3 (enExample)
ES (2) ES2665645T3 (enExample)
HU (2) HUE057119T2 (enExample)
IL (1) IL242408B (enExample)
MX (1) MX368177B (enExample)
NO (1) NO2994485T3 (enExample)
RU (1) RU2662668C2 (enExample)
SG (1) SG11201508968XA (enExample)
TW (2) TWI675041B (enExample)
WO (1) WO2014180852A1 (enExample)

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KR20220069043A (ko) * 2019-09-23 2022-05-26 머크 샤프 앤드 돔 코포레이션 감소된 숙주 세포 단백질 및 증가된 폴리소르베이트-80 안정성을 갖는 항-ctla4 모노클로날 항체를 포함하는 방법 및 조성물
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WO2014180852A1 (en) 2014-11-13
AU2014264697A1 (en) 2015-11-26
US10793622B2 (en) 2020-10-06
RU2662668C2 (ru) 2018-07-26
EP3301115B1 (en) 2021-11-10
CN105358572A (zh) 2016-02-24
DK2994485T3 (en) 2018-04-23
EP2994485B1 (en) 2018-01-10
US20160083454A1 (en) 2016-03-24
JP6463734B2 (ja) 2019-02-06
HUE038741T2 (hu) 2018-11-28
IL242408B (en) 2020-04-30
ES2905675T3 (es) 2022-04-11
BR112015027812B1 (pt) 2021-03-30
TWI631132B (zh) 2018-08-01
ES2665645T3 (es) 2018-04-26
AU2014264697B2 (en) 2019-08-22
BR112015027812A2 (pt) 2017-07-25
CA2911462C (en) 2021-04-06
CN116003579A (zh) 2023-04-25
TW201908331A (zh) 2019-03-01
SG11201508968XA (en) 2015-11-27
CY1125281T1 (el) 2023-06-09
RU2015151869A (ru) 2017-06-13
HUE057119T2 (hu) 2022-04-28
MX368177B (es) 2019-09-23
JP2019104735A (ja) 2019-06-27
MX2015015482A (es) 2016-03-21
EP2994485A1 (en) 2016-03-16
TWI675041B (zh) 2019-10-21
EP3301115A1 (en) 2018-04-04
JP2016519137A (ja) 2016-06-30
CA2911462A1 (en) 2014-11-13
DK3301115T3 (da) 2022-01-31
NO2994485T3 (enExample) 2018-06-09
KR20160005047A (ko) 2016-01-13
TW201522363A (zh) 2015-06-16

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