KR102253532B1 - 낮은 ph에서 발효에 의한 유기산의 제조 - Google Patents

낮은 ph에서 발효에 의한 유기산의 제조 Download PDF

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KR102253532B1
KR102253532B1 KR1020157009348A KR20157009348A KR102253532B1 KR 102253532 B1 KR102253532 B1 KR 102253532B1 KR 1020157009348 A KR1020157009348 A KR 1020157009348A KR 20157009348 A KR20157009348 A KR 20157009348A KR 102253532 B1 KR102253532 B1 KR 102253532B1
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genetically engineered
succinate
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engineered yeast
gene
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KR20150055006A (ko
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알. 로져스 요쿰
수드한슈 돌
재니스 지. 페로
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피티티 글로벌 케미컬 퍼블릭 컴퍼니 리미티드
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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KR1020157009348A 2012-09-14 2013-09-13 낮은 ph에서 발효에 의한 유기산의 제조 Active KR102253532B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261701293P 2012-09-14 2012-09-14
US61/701,293 2012-09-14
PCT/US2013/059828 WO2014043591A1 (en) 2012-09-14 2013-09-13 Production of organic acids by fermentation at low ph

Publications (2)

Publication Number Publication Date
KR20150055006A KR20150055006A (ko) 2015-05-20
KR102253532B1 true KR102253532B1 (ko) 2021-05-18

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US (1) US11072807B2 (https=)
EP (1) EP2895593B1 (https=)
JP (3) JP2015528312A (https=)
KR (1) KR102253532B1 (https=)
CN (1) CN104812889B (https=)
BR (1) BR112015005416A2 (https=)
CA (1) CA2884266C (https=)
MY (1) MY182946A (https=)
WO (1) WO2014043591A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2687602A1 (en) 2007-11-20 2014-01-22 DSM IP Assets B.V. Succinic acid production in a eukaryotic cell
KR102311681B1 (ko) * 2015-07-28 2021-10-12 삼성전자주식회사 내산성을 갖는 효모 세포, 그를 이용하여 유기산을 생산하는 방법 및 상기 내산성 효모 세포를 생산하는 방법
KR20190003939A (ko) * 2016-03-02 2019-01-10 피티티지씨 이노베이션 아메리카 코포레이션 유전자 조작된 미생물로부터 개선된 뮤콘산 생산
EP3267345A1 (en) * 2016-07-06 2018-01-10 European Molecular Biology Laboratory Methods for adaptive laboratory evolution
CA3068443A1 (en) 2017-06-30 2019-01-03 Ptt Global Chemical Public Company Limited Genetically modified yeast with increased succinic acid production
EP3645725A1 (en) 2017-06-30 2020-05-06 PTT Global Chemical Public Company Limited Microorganism with stabilized copy number of functional dna sequence and associated methods
WO2019159011A2 (en) 2018-02-16 2019-08-22 Ptt Global Chemical Public Company Limited Microorganisms and processes for lactic acid production
CN108676736B (zh) * 2018-04-20 2020-05-19 华中农业大学 一株可降解柠檬酸的毕赤酵母菌及其应用
US12209236B2 (en) * 2018-05-25 2025-01-28 Danisco Us Inc. Overexpression of fumarate reductase results in an increased fermentation rate in yeast
EP4017515B1 (en) * 2019-08-19 2025-06-25 PTT Global Chemical Public Company Limited Yeast having improvement of lactic acid tolerance and use thereof
CN117467551A (zh) * 2022-07-29 2024-01-30 中国科学院天津工业生物技术研究所 一种高产l-苹果酸的耐酸酵母菌株及其构建方法和应用
CN119875861A (zh) * 2023-10-24 2025-04-25 中国石油化工股份有限公司 一株耐酸马克斯克鲁维酵母菌株及其在生产丁二酸中的应用
CN119162073A (zh) * 2024-11-01 2024-12-20 湖南省微生物研究院 一株柠檬酸杆菌基因工程菌株及其构建方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012103261A2 (en) 2011-01-25 2012-08-02 Finley Kenneth R Compositions and methods for succinate production
US20120225461A1 (en) 2009-11-18 2012-09-06 Myriant Corporation Organic acid production in microorganisms by combined reductive and oxidative tricaboxylic acid cylce pathways

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294728B1 (it) 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
US20070031950A1 (en) 1998-09-11 2007-02-08 Winkler Aaron A Production of D-lactic acid with yeast
US6485947B1 (en) 1999-05-21 2002-11-26 Cargill Dow Polymers, Llc Production of lactate using crabtree negative organisms in varying culture conditions
US7141410B2 (en) 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
RU2182689C1 (ru) 2001-06-14 2002-05-20 Юрий Эдуардович Зевацкий Способ и устройство для охлаждения рабочего тела и способ генерации микроволнового излучения
EP1513939B1 (en) * 2002-05-30 2014-12-31 Cargill Inc. Fermentation process using specific oxygen uptake rates as a process control
BRPI0610951A2 (pt) 2005-06-02 2010-08-03 Cargill Inc levedura geneticamente modificada da espécie issatchenkia orientalis e espécies estreitamente relacionadas e processos de fermentação utilizando as mesmas
JP2009507493A (ja) * 2005-09-09 2009-02-26 ジェノマティカ・インコーポレイテッド 増殖連動型のコハク酸塩生成のための方法と生物
WO2007038130A2 (en) 2005-09-22 2007-04-05 Tate & Lyle Ingredients Americas, Inc. Improved strains for the production of organic acids
ES2527980T3 (es) 2005-10-14 2015-02-02 Toray Industries, Inc. Levadura y procedimiento de producción de ácido L-láctico
US20080090273A1 (en) * 2005-11-21 2008-04-17 Aaron Adriaan Winkler Malic Acid Production in Recombinant Yeast
US8137953B2 (en) 2005-11-23 2012-03-20 Cargill Inc. Lactic acid-producing yeast cells having nonfunctional L- or D-lactate:ferricytochrome C oxidoreductase cells
DE102007019184A1 (de) * 2007-04-20 2008-10-23 Organo Balance Gmbh Mikroorganismus zur Herstellung von Bernsteinsäure
EP2158323A4 (en) 2007-05-18 2011-06-22 Microbia Prec Engineering Inc PRODUCTION OF ORGANIC ACID BY PILZ CELLS
US20100248233A1 (en) 2007-07-23 2010-09-30 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast
EP2220234B1 (en) 2007-11-20 2012-06-27 DSM IP Assets B.V. Dicarboxylic acid production in a recombinant yeast
EP2687602A1 (en) 2007-11-20 2014-01-22 DSM IP Assets B.V. Succinic acid production in a eukaryotic cell
EP2222854A1 (en) 2007-11-20 2010-09-01 DSM IP Assets B.V. Dicarboxylic acid production in a filamentous fungus
US20110104771A1 (en) 2008-02-15 2011-05-05 Rene Verwall Process for the production of a dicarboxylic acid
ES2623932T3 (es) 2008-07-08 2017-07-12 Dsm Ip Assets B.V. Producción de ácido succínico por fermentación a bajo pH
DE102008051727A1 (de) 2008-10-17 2010-04-22 Organo Balance Gmbh Mikroorganismus zur Herstellung von Bernsteinsäure
US20100104771A1 (en) 2008-10-24 2010-04-29 Applied Materials, Inc. Electrode and power coupling scheme for uniform process in a large-area pecvd chamber
RU2422526C2 (ru) 2009-01-30 2011-06-27 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов (ФГУП ГосНИИгенетика) СПОСОБ ПОЛУЧЕНИЯ ЯНТАРНОЙ КИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ ДРОЖЖЕЙ, ПРИНАДЛЕЖАЩИХ К РОДУ Yarrowia
AU2010232533A1 (en) * 2009-04-02 2011-10-13 University Of Florida Research Foundation, Inc. Engineering the pathway for succinate production
WO2010118932A1 (en) 2009-04-15 2010-10-21 Dsm Ip Assets B.V. Dicarboxylic acid production process
CN102575235B (zh) * 2009-07-30 2015-05-20 代谢探索者公司 用于通过发酵生产生物化学品的突变甲基乙二醛合酶(mgs)
US9783834B2 (en) 2009-08-27 2017-10-10 Dsm Ip Assets B.V. Dicarboxylic acid fermentation process
US8945140B2 (en) * 2010-06-18 2015-02-03 Vantage Surgical Systems, Inc. Surgical procedures using instrument to boundary spacing information extracted from real-time diagnostic scan data
CA2811539A1 (en) 2010-09-24 2012-03-29 Dsm Ip Assets B.V. Dicarboxylic acid production process
EP2495304A1 (en) * 2010-12-03 2012-09-05 DSM IP Assets B.V. Dicarboxylic acid production in a yeast cell
WO2012103263A2 (en) 2011-01-25 2012-08-02 Finley Kenneth R Compositions and methods for malate and fumarate production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120225461A1 (en) 2009-11-18 2012-09-06 Myriant Corporation Organic acid production in microorganisms by combined reductive and oxidative tricaboxylic acid cylce pathways
WO2012103261A2 (en) 2011-01-25 2012-08-02 Finley Kenneth R Compositions and methods for succinate production

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JP6879976B2 (ja) 2021-06-02
EP2895593B1 (en) 2019-12-11
JP2020127417A (ja) 2020-08-27
CN104812889A (zh) 2015-07-29
CA2884266C (en) 2022-05-24
CN104812889B (zh) 2018-08-28
BR112015005416A2 (pt) 2017-08-08
MY182946A (en) 2021-02-05
US20150240270A1 (en) 2015-08-27
EP2895593A1 (en) 2015-07-22
US11072807B2 (en) 2021-07-27
EP2895593A4 (en) 2016-06-22
JP2018134108A (ja) 2018-08-30
KR20150055006A (ko) 2015-05-20
JP2015528312A (ja) 2015-09-28
WO2014043591A1 (en) 2014-03-20
CA2884266A1 (en) 2014-03-20

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