KR102248730B1 - 유전자 표적화를 위한 범용 공여자 시스템 - Google Patents

유전자 표적화를 위한 범용 공여자 시스템 Download PDF

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KR102248730B1
KR102248730B1 KR1020167014602A KR20167014602A KR102248730B1 KR 102248730 B1 KR102248730 B1 KR 102248730B1 KR 1020167014602 A KR1020167014602 A KR 1020167014602A KR 20167014602 A KR20167014602 A KR 20167014602A KR 102248730 B1 KR102248730 B1 KR 102248730B1
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sequence
polynucleotide
dna
plant
gene
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KR20160079090A (ko
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라크쉬미 사스트리-덴트
스티븐 엘 에반스
라이언 시 블루
제후이 차오
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코르테바 애그리사이언스 엘엘씨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020167014602A 2013-11-04 2014-11-03 유전자 표적화를 위한 범용 공여자 시스템 Expired - Fee Related KR102248730B1 (ko)

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Application Number Priority Date Filing Date Title
US201361899543P 2013-11-04 2013-11-04
US61/899,543 2013-11-04
PCT/US2014/063732 WO2015066637A1 (en) 2013-11-04 2014-11-03 A universal donor system for gene targeting

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KR20160079090A KR20160079090A (ko) 2016-07-05
KR102248730B1 true KR102248730B1 (ko) 2021-05-07

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US (1) US10077449B2 (https=)
EP (1) EP3066192B1 (https=)
JP (1) JP6560203B2 (https=)
KR (1) KR102248730B1 (https=)
CN (1) CN105683357B (https=)
AR (1) AR098283A1 (https=)
AU (1) AU2014341928B2 (https=)
BR (1) BR102014027448A8 (https=)
CA (1) CA2926534A1 (https=)
IL (1) IL245313B (https=)
RU (1) RU2687369C2 (https=)
TW (1) TWI669395B (https=)
WO (1) WO2015066637A1 (https=)
ZA (1) ZA201601929B (https=)

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US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
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AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
CN108064129A (zh) 2014-09-12 2018-05-22 纳幕尔杜邦公司 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法
US10900034B2 (en) 2014-12-03 2021-01-26 Agilent Technologies, Inc. Guide RNA with chemical modifications
BR112017015988B1 (pt) 2015-01-27 2024-02-27 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Métodos para a modificação sítio dirigida para um fragmento alvo de um gene alvo em uma planta inteira e para a obtenção de uma planta mutante livre de transgene
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Also Published As

Publication number Publication date
KR20160079090A (ko) 2016-07-05
IL245313B (en) 2019-05-30
CA2926534A1 (en) 2015-05-07
TWI669395B (zh) 2019-08-21
RU2687369C2 (ru) 2019-05-13
EP3066192A4 (en) 2017-07-19
US20150143588A1 (en) 2015-05-21
EP3066192A1 (en) 2016-09-14
TW201522630A (zh) 2015-06-16
WO2015066637A1 (en) 2015-05-07
ZA201601929B (en) 2019-06-26
JP2016534727A (ja) 2016-11-10
CN105683357B (zh) 2020-10-30
AU2014341928A1 (en) 2016-04-07
IL245313A0 (en) 2016-06-30
BR102014027448A8 (pt) 2021-08-24
AR098283A1 (es) 2016-05-26
US10077449B2 (en) 2018-09-18
CN105683357A (zh) 2016-06-15
BR102014027448A2 (pt) 2015-09-15
JP6560203B2 (ja) 2019-08-14
EP3066192B1 (en) 2019-05-01
RU2016122038A (ru) 2017-12-11
AU2014341928B2 (en) 2017-11-30
RU2016122038A3 (https=) 2018-06-21

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