KR102196459B1 - A toothpaste composition with improved antibacterial performance and a toothpaste comprising the composition - Google Patents

Abstract

The present disclosure is a toothpaste composition with improved antibacterial performance, wherein the water includes ionic mineral raw material water; abrasive; Humectants; Thickener; Tocopherol acetate, aminocaproic acid, or a combination thereof; And an additive, wherein the toothpaste composition includes a toothpaste composition with improved antibacterial performance that inhibits the culture of oral bacteria by 95% or more when the toothpaste composition is applied to oral bacteria and 24 hours elapse, and the toothpaste composition Provides.

Description

A toothpaste composition with improved antibacterial performance and a toothpaste comprising the composition}

The present disclosure relates to a toothpaste composition with improved antibacterial performance, and more particularly, to a toothpaste composition that effectively inhibits the growth of oral bacteria for 24 hours after brushing as well as immediately after brushing.

In general, toothpaste is an oral cleaning agent used for cleaning the oral cavity and teeth, and is manufactured in various forms such as paste toothpaste, liquid toothpaste, and powder toothpaste. Toothpaste is used in the oral cavity to disinfect, remove odor, dissolve tartar, and prevent tooth decay.

In order to meet the needs of consumers, modern toothpaste is being commercialized as a functional toothpaste that has not only the general effects of the conventional toothpaste, but also additional effects such as prevention of periodontal disease and promotion of human metabolism. Research and development for such functional toothpaste is being actively conducted.

However, in order to achieve the above effects, a number of chemical components that may be harmful to the human body are contained in toothpaste. There is room for improvement in that the use of toothpaste in general consumers, especially children whose impact on harmful substances to the human body is fatal, has the potential to have adverse effects in the future.

On the other hand, in the functional toothpaste for preventing periodontal disease, the antibacterial activity to inhibit the growth of oral bacteria and the persistence of such antibacterial activity correspond to important factors. Conventional toothpaste claims an effect on the antibacterial activity immediately after brushing and on the antibacterial activity after a short period of time, but this is only an experiment performed in vitro or a non-human animal. In addition, there is no known toothpaste with antibacterial activity that is maintained for a long time, such as 24 hours.

For example, prior art KR 10-1221074 discloses a natural antibacterial children's toothpaste composition that provides an oral antibacterial effect. However, in the case of the prior art KR 10-1221074, the results of clinical trials in humans were not disclosed, and the course of the antibacterial test in the body was not observed, and the observation time was only 18 hours.

In addition, in the case of the prior art toothpaste composition, the SiO ₂ component is contained in an excess of 63% or more.SiO ₂ is added in the form of dental-type silica as an abrasive in the conventional toothpaste, but the excessive addition of SiO ₂ It can cause wear of teeth, which is not desirable for the health of teeth from a long-term perspective.

Under this background, development and research of toothpaste with improved eco-friendly and antibacterial performance has been conducted while being freed from the fear of chemical substances.

KR 10-1221074

Accordingly, a first aspect of the present disclosure is to provide a highly functional toothpaste composition that is not harmful to the human body, is environmentally friendly, and has improved antibacterial performance.

A second aspect of the present disclosure is to provide a highly functional toothpaste comprising the toothpaste composition of the first aspect.

A toothpaste composition with improved antibacterial performance for achieving the first aspect of the present disclosure is water, wherein the water comprises ionic mineral raw material water; abrasive; Humectants; Thickener; Tocopherol acetate, aminocaproic acid, or a combination thereof; And an additive, wherein the toothpaste composition inhibits the cultivation of oral bacteria by 95% or more when the toothpaste composition is applied to oral bacteria and 24 hours elapse.

According to one embodiment of the present disclosure, the ionic mineral raw material water includes magnesium, silicon, calcium, and potassium.

According to an embodiment of the present disclosure, the ionic mineral raw material water includes 2 to 5 mg of SiO ₂ per 1 L of the ionic mineral raw water.

According to an embodiment of the present disclosure, the dentifrice composition comprises 10 to 20% by weight of an abrasive, 60 to 70% by weight of a wetting agent, and 0.5 to 2% by weight of a thickener based on the total weight of the toothpaste composition, wherein the The abrasive is dental type silica; The humectant is D-sorbitol liquid, concentrated glycerin, or a combination thereof; The thickener is xanthan gum.

According to an embodiment of the present disclosure, the additive is selected from the group consisting of grapefruit seed extract, matricaria extract, aloe extract, eucalyptus extract, sage extract, xylitol, ascorbic acid, sodium cocoyl glutamate, and combinations thereof. It is one.

According to an embodiment of the present disclosure, the toothpaste composition further includes other additives, wherein the other additives include pogongyeong extract, propolis extract, sodium chloride, mastic oil, green tea extract, mint flavor additive, and combinations thereof. It is 1 type selected from the group consisting of.

According to one embodiment of the present disclosure, the toothpaste composition further includes other additives, wherein the other additive is one selected from the group consisting of calendula extract, spearmint oil, fennel oil, and combinations thereof.

According to one embodiment of the present disclosure, the toothpaste composition does not contain harmful ingredients, and the harmful ingredients are fluorine, synthetic surfactants, triclosan, MIT (methylisothiazolinone), CMIT (Methylchloroisothiazolinone), PEG (Polyethylene glycol), precipitated carbonic acid. Calcium, parabens, saccharin, and tar pigments.

According to an embodiment of the present disclosure, the oral bacteria are Campylobacter rectus (Campylobacter rectus), Fusobacterium nucleatum, Porphyromonas gingivalis (Porphyromonas gingivalis), Agregati vector Actinomycetemcomitans (Aggregatibacter actinomycetemcomitans), Streptococcus mutans, Streptococcus sobrinus, Eikenella corrodens, and Prevotella intermedia intermedia).

The toothpaste for achieving the second aspect of the present disclosure includes the toothpaste composition of the first aspect.

The toothpaste composition according to the present disclosure does not contain any harmful ingredients and guarantees enhanced safety for the human body by using natural ingredients. In addition, it has an advantageous effect of excellently removing oral bacteria from the viewpoint of antibacterial properties, and inhibiting the cultivation and reproduction of oral bacteria for more than 24 hours. From a long-term perspective, it has an advantageous effect of improving the user's oral environment to strengthen the gum tissue and prevent periodontal disease.

In addition, in the case of toothpaste containing the toothpaste composition according to the present disclosure, the toothpaste containing the toothpaste composition according to the present disclosure, unlike the conventional toothpaste that makes the tongue feel excessively spicy in order to achieve disinfection, odor removal, tartar dissolution, and tooth decay prevention. It has the advantage of easy accessibility to users, especially those of the lower age group, who have strong resistance to spicy taste, by minimizing the roughness after brushing without feeling any spicy taste at all and allowing a sufficient refreshing feeling to be felt.

1 shows positions of photographed images of the dishes of FIGS. 2 to 9 taken by the antibacterial activity test of the present disclosure by time;
2 to 9 are photographed images of a dish after 2, 4, 6, 8, and 24 hours have elapsed in the antibacterial activity test of the present disclosure;
10 to 12 are graphs plotting the results of the Human saliva test of the present disclosure; And
13 shows the results of the Human-derived micro-organism test of the present disclosure.

Objects, specific advantages, and novel features of the present disclosure will become more apparent from the following detailed description and preferred embodiments associated with the accompanying drawings, but the present disclosure is not necessarily limited thereto. In addition, in describing the present disclosure, if it is determined that a detailed description of related known technologies may unnecessarily obscure the subject matter of the present disclosure, the detailed description thereof will be omitted.

The term "mineral raw material water" as used in the present disclosure means mineral water containing minerals included as a raw material in the toothpaste of the present disclosure.

The term "antibacterial activity" as used in the present disclosure generally refers to the ability to remove bacteria or to inhibit its reproduction and development, and more specifically, in the present disclosure, refers to the ability to remove or inhibit the reproduction and development of oral bacteria. do.

The present disclosure provides a toothpaste composition with improved antibacterial performance. The toothpaste composition is water; abrasive; Humectants; Thickener; Tocopherol acetate, aminocaproic acid, or a combination thereof; And additives.

The toothpaste composition generally contains water. In the conventional toothpaste composition, purified water has been generally used as the water, and a case of utilizing mineral water as water included in the toothpaste composition has not yet been found.

In contrast, the toothpaste composition according to the present disclosure includes water, and the water includes mineral water. The mineral raw material water means water containing minerals such as calcium, magnesium, potassium, and silicon. Minerals can be largely divided into ionic minerals in an ionic state and inorganic minerals in a monoatomic state. Humans cannot convert inorganic minerals into ionic minerals in the body, and therefore, inorganic minerals are difficult to be absorbed in the body, and ingestion of inorganic mineral water has a limitation in that it is difficult to see beneficial effects on the human body. In contrast, in the case of ionic minerals, since the mineral is completely dissolved in water in the form of ions, it has an advantageous advantage that it can be immediately absorbed in the human body. Accordingly, the toothpaste composition of the present disclosure uses ionic mineral raw water among mineral raw water.

The ionic mineral raw material water may include magnesium, silicon, calcium, and potassium as minerals. The magnesium may be contained 1 to 5 mg per 1 L of ionic mineral raw material water, such as 1.5 to 4 mg, 2 to 3.5 mg, 2 to 3 mg, and 2.5 to 3 mg. Magnesium in the ionic mineral raw material water has the effect of strengthening the enamel (enamel) of teeth.

In addition, the ionic mineral raw material water of the present disclosure may contain the silicon in the form of silicon dioxide. The silicon dioxide may contain 2 to 6 mg, such as 2 to 4.5 mg, 2 to 4 mg, 2.5 to 4.5 mg, 2.5 to 4 mg, 3 to 4.5 mg, 3 to 4 mg, per 1 L of ionic mineral raw material water. have. Silicon in the ionic mineral raw material water is advantageous in terms of enhancing the oral antibacterial activity of the toothpaste composition and reinforcing the bone density of the user's teeth.

In addition, the ionic mineral raw material water of the present disclosure may include 4 to 10 mg, more preferably 4 to 9 mg, of the calcium per 1 L of the ionic mineral raw water. Calcium in the ionic mineral raw material water may have a positive effect on the maintenance and recovery of tooth strength of a toothpaste user.

In addition, the ionic mineral raw material water of the present disclosure contains the potassium per 1L of the ionic mineral raw water, such as 0.2 to 1.9 mg, 0.2 to 1.8 mg, 0.2 to 1.7 mg, 0.2 to 1.6 mg, 0.2 to 1.5 mg, 0.2 to 1.4 mg , 0.3 to 1 mg, 0.3 to 0.9 mg, 0.3 to 0.8 mg, 0.3 to 0.7 mg, 0.3 to 0.6 mg, 0.3 to 0.5 mg, may contain 0.2 to 2 mg such as 0.3 to 0.4 mg. The potassium has the effect of strengthening the oral immunity of the toothpaste user.

The ionic mineral raw material water of the present disclosure may further contain strontium. The strontium has a positive effect on bone density and cartilage of toothpaste users, and contributes to the prevention of osteoporosis.

Table 1 below shows the mineral content per 1L of the ionic mineral raw material water contained in the toothpaste composition according to an embodiment of the present disclosure. The unit of each of the mineral components is mg. It should be noted that the specific examples in Table 1 below are exemplary, and the composition of the ionic mineral raw material water of the present disclosure is not limited thereto.

ingredient Content(mg) K 0.33 Na 4.08 Ca 8.69 Mg 2.72 SiO ₂ 3.83 Li Less than 0.01 Sr 0.17 Fe Less than 0.03 Mn Less than 0.01 Cu Less than 0.03 Pb Less than 0.02 Zn 0.25 F ^- 1.32 Cl ^- 5.34 SO4 ^2- 36.8

According to an embodiment of the present disclosure, water included in the toothpaste composition may be ionic mineral raw material water. According to another embodiment of the present disclosure, the water may be a mixture of purified water and ionic mineral raw water. In this case, the purified water and the ionic mineral raw material water may be mixed and used in a volume ratio of 10 to 14 to 1, preferably 11 to 13 to 1, and more preferably 12 to 1.

The toothpaste composition of the present disclosure may include an abrasive. The'polishing agent' refers to a material that functions to remove plaque from the oral cavity, and refers to a material commonly included in toothpaste to increase removal efficiency of tooth plugs and to remove hard foreign substances. The abrasive is, for example, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, calcium pyrophosphate, insoluble metaphosphate, silica, dental type silica, silica gel, precipitated silica, fumed silica, colloidal silica, zirconium silicate, aluminum silicate, zeolite, Calcium silicate, aluminum silicate, aluminum oxide, aluminum hydroxide, zirconium silicate, calcium carbonate, hydrous alumina, kaolin, and mixtures thereof. In the present disclosure, the abrasive may preferably be dental type silica. The dental-type silica has no salty taste and has a neutral pH, so it does not irritate the oral mucosa, so toothpaste users can use it without any particular limitation. Furthermore, the dental type silica has a positive effect on enhancing the antibacterial activity of the toothpaste composition and preventing gum disease of the user.

According to one embodiment of the present disclosure, the abrasive may be included in 10 to 20% by weight based on the total weight of the toothpaste composition. The abrasive may preferably be 10 to 18% by weight, more preferably 10 to 15% by weight, most preferably 12 to 15% by weight, based on the total weight of the toothpaste composition. If the abrasive is included in an amount of less than 10% by weight, the amount of the abrasive contained in the toothpaste composition is too small, and thus a problem may arise that it is not effective in removing the plug of the tooth. On the other hand, when the abrasive is included in an amount of more than 20% by weight, the abrasive may wear not only the tooth plug but also the tooth itself, thereby causing a problem of damaging dental health. In particular, in the case of children 12 years or younger, it is preferable that the content of the abrasive is about 12% by weight for long-term dental health management.

The dentifrice composition of the present disclosure may also include a humectant. The'wetting agent' serves to maintain moisture in the toothpaste composition by absorbing water contained in the toothpaste composition. The wetting agent applicable in the present disclosure is not particularly limited as long as it can perform the above-described role and does not negatively affect the effect of the toothpaste composition of the present disclosure. For example, as the wetting agent, concentrated glycerin, glycerin, sorbitol, amorphous sorbitol, polyethylene glycol, propylene glycol, ethylene glycol, polypropylene glycol, or a mixture thereof may be used. Preferably, the humectant may be D-sorbitol liquid, concentrated glycerin, or a combination thereof.

The toothpaste composition of the present disclosure may contain 60 to 70% by weight of the humectant based on the total weight of the toothpaste composition. The humectant may preferably contain 62 to 68% by weight, more preferably 65 to 68% by weight, based on the total weight of the toothpaste composition. According to one embodiment of the present disclosure, the toothpaste composition includes D-sorbitol liquid as a humectant, and the humectant may be 68% by weight based on the total weight of the toothpaste composition. According to another embodiment of the present disclosure, the toothpaste composition comprises D-sorbitol liquid and concentrated glycerin as a wetting agent, and the content of the D-sorbitol liquid based on the total weight of the toothpaste composition is 65% by weight, and the concentrated glycerin The content may be 3% by weight.

The toothpaste composition of the present disclosure may include a thickening agent. The'thickener' serves to prevent the separation of the solid component and the liquid component of the toothpaste, and is not particularly limited as long as it can perform the same role and does not interfere with the achievement of the effect of the toothpaste composition to be provided in the present disclosure. . The thickener may be, for example, methyl cellulose, carboxymethyl cellulose, ethyl cellulose, hydroxypropyl cellulose, and xanthan gum. In the toothpaste composition of the present disclosure, in particular, xanthan gum, which is a thickener of natural ingredients extracted from sugar cane, is environmentally friendly and is preferable from the viewpoint of minimizing harmful ingredients to the human body. The thickener may be included in an amount of 0.5 to 2% by weight based on the total weight of the toothpaste composition. The thickener may preferably be included in 0.5 to 1.5% by weight based on the total weight of the toothpaste composition, more preferably 0.7 to 1.0% by weight, such as 0.8% by weight, 0.9% by weight.

The toothpaste composition of the present disclosure may contain tocopherol acetate, aminocaproic acid, or a combination thereof. The tocopherol acetate (vitamin E) helps hemostasis by activating proteolytic enzymes that generate collagen in the gums to help restore wounds on the gums, and to coagulate blood. Tocopherol acetate contained in the toothpaste composition of the present disclosure contributes to alleviation of symptoms of gum disease such as inflammation, swelling, pain and bleeding of the gums, and further contributes to the prevention of tooth decay and gum disease. In addition, since the aminocaproic acid has excellent anti-bleeding and anti-inflammatory effects, it may be included in the toothpaste composition of the present disclosure, thereby contributing to preventing gingival bleeding and gum disease. According to one embodiment of the present disclosure, the toothpaste composition may include tocopherol acetate. According to another embodiment of the present disclosure, the toothpaste composition may include tocopherol acetate and aminocaproic acid.

The content of tocopherol acetate, aminocaproic acid, and a combination thereof in the toothpaste composition is 0.05 to 0.5% by weight, preferably 0.05 to 0.3% by weight, more preferably 0.05 to 0.2% by weight based on the total weight of the toothpaste composition. I can. According to one embodiment of the present disclosure, the toothpaste composition may include 0.1% by weight of aminocaproic acid and 0.01% by weight of tocopherol acetate. According to another embodiment of the present disclosure, the toothpaste composition may contain 0.1% by weight of tocopherol acetate, and may not contain aminocaproic acid.

The toothpaste composition of the present disclosure may contain an additive. The additive may be one selected from the group consisting of grapefruit seed extract, matricaria extract, aloe extract, eucalyptus extract, sage extract, xylitol, ascorbic acid (vitamin C), sodium cocoyl glutamate, and combinations thereof. Preferably, the toothpaste composition of the present disclosure may include grapefruit seed extract, matricaria extract, aloe extract, eucalyptus extract, sage extract, xylitol, ascorbic acid (vitamin C), and sodium cocoyl glutamate as additives.

As an additive added to the toothpaste composition of the present disclosure, the grapefruit seed extract functions as a naturally derived preservative that inhibits oral bacteria and prevents plaque, tooth decay, and gingivitis. In addition, Matricaria extract is extracted from chamomile flowers, has a function of anti-allergic and midnight, and serves to relieve irritation caused by toothpaste to users. In addition, the aloe extract extracted from aloe leaves has antibacterial, antiviral, analgesic, wound healing and anti-inflammatory functions. Eucalyptus extract functions to prevent tooth decay and gum disease, and sage extract functions as an antiseptic, antibacterial, and anti-inflammatory. In addition, xylitol contributes to the prevention of tooth decay, and ascorbic acid, vitamin C, prevents gum disease and has an antioxidant function. In addition, sodium cocoyl glutamate functions as a tooth cleaning and natural foaming agent.

The toothpaste composition may contain an additive in an amount greater than 0 to 6% by weight or less based on the total weight of the toothpaste composition. More preferably, the additive may be included in the toothpaste composition in an amount of more than 1 to 5.8% by weight or less. Specifically, according to one embodiment of the present disclosure, the toothpaste composition is a grapefruit seed extract greater than 0 to 0.3% by weight or less, Matricaria extract greater than 0 to 0.1% by weight, aloe extract greater than 0 to 0.1% by weight or less, eucalyptus extract More than 0 to 0.1% by weight or less, more than 0 to 0.1% by weight of sage extract, more than 0 to 2% by weight of xylitol, more than 0 to 0.05% by weight of ascorbic acid, and more than 1 to 3% by weight of sodium cocoyl glutamate. Can include.

The toothpaste composition of the present disclosure may further contain other additives. The other additives may include pogongyeong extract, propolis extract, sodium chloride, mastic oil, green tea extract, mint flavor additive, calendula extract, spearmint oil, and fennel oil.

Pogongyeong contains substances such as taraxin, choline, inulin, pectin, achillin, and leucodine, among which achillin and leukodin have the effect of suppressing allergic reactions. In oriental medicine, it is known that pogongyeong was used to cool down heat as a treatment for boils. The pogongyoung is included in the toothpaste composition of the present disclosure and thus functions to relieve allergy and heat.

Propolis extract strengthens gums and has anti-inflammatory properties. By being included in the toothpaste composition of the present disclosure, the propolis extract contributes to the prevention of tooth decay, gingivitis, and periodontitis, and further contributes to additional effects such as plaque removal, whitening effect, and bad breath removal.

Sodium chloride contained in the toothpaste composition of the present disclosure contributes to the prevention of gingivitis and periodontitis, and mastic oil serves to prevent Helicobacter bacteria and periodontal disease bacteria. In addition, green tea extract contains an antioxidant component called polyphenol, which is effective in removing bad breath, and the flavonoid component suppresses the growth of oral bacteria. The mint flavor additive can provide a refreshing feeling to the user.

The calendula extract, which may be included as other additives in the toothpaste composition of the present disclosure, has anti-acid and antibacterial effects, and contributes to the improvement of transparency, foaming power, and stability of toothpaste, as well as caries and gum disease prevention properties. Furthermore, the calendula extract helps to heal wounds on the gums. In addition, spearmint oil provides a refreshing sensation, and is milder and less irritating than peppermint, so it may be more suitable when a young child is a user. In addition, in the case of fennel oil, it is possible to replace the conventional artificial fruit flavor while providing a refreshing feeling as a natural herbal component, so that the content of artificial additives in toothpaste for children can be further reduced. Furthermore, the fennel oil may provide side effects such as loss of appetite and improvement of cold hands and feet.

According to one embodiment of the present disclosure, the other additive may be one selected from the group consisting of pogongyoung extract, propolis extract, sodium chloride, mastic oil, green tea extract, mint flavor additive, and combinations thereof. According to another embodiment of the present disclosure, the other additive may be one selected from the group consisting of calendula extract, spearmint oil, fennel oil, and combinations thereof.

The toothpaste composition of the present disclosure may include more than 0 to 2% by weight of other additives based on the total weight of the toothpaste composition. Preferably, the other additives may be included in an amount of more than 0 to 1.75% by weight. More preferably, the other additives may be included in an amount greater than 0 to 1% by weight. Specifically, according to one embodiment of the present disclosure, the toothpaste composition is other additives, pogongyoung extract 0 to 0.05 wt%, propolis extract 0 to 0.05 wt%, sodium chloride 0 to 0.2 wt%, mastic oil 0 More than 0.05% by weight, more than 0 to 0.1% by weight of green tea extract, more than 0 to 1% by weight of mint flavor additive, more than 0 to 0.1% by weight of calendula extract, from more than 0 to 0.1% by weight of spearmint oil, and from more than 0 to fennel oil It may contain 0.1% by weight.

The toothpaste composition of the present disclosure does not contain harmful ingredients. The harmful component is not particularly limited as long as it is a component known to be harmful to the human body, but in the present disclosure, in particular, fluorine, synthetic surfactant, triclosan, MIT (Methylisothiazolinone), CMIT (Methylchloroisothiazolinone), PEG (Polyethylene glycol), precipitated calcium carbonate, paraben, Saccharin, and tar pigments.

Fluoride has been contained in conventional toothpaste to prevent tooth decay, but this fluoride is not preferable because there is a concern that it may cause tooth fluorosis to stain the tooth surface or severely discolor when excessive intake by users, especially children. In addition, synthetic surfactants have a function of foaming in conventional toothpaste, but if these synthetic surfactants are not completely removed through washing in the oral cavity, there is a problem that it may cause bad breath by drying the oral mucosa. Triclosan is a fungicide used to inhibit the proliferation of microorganisms in the oral cavity, and has been widely used in conventional toothpastes, but this triclosan is an endocrine disrupting substance that lowers immunity and interferes with the correct functioning of hormones, that is, environmental hormones. The triclosan may cause infertility, breast cancer, liver cancer, and hypothyroidism. MIT and CMIT are harmful ingredients that are well-known as raw materials for humidifier disinfectants, and are highly toxic and have a problem of causing skin corrosion. PEG is used in conventional toothpaste as a surfactant, but it is a problem in many cases where carcinogens such as 1,4 dioxane remain in the process for improving the cleaning and emulsifying power of the PEG. In order to add color to the toothpaste, tar pigments were used in conventional toothpastes, but the tar pigments often contain carcinogens, causing a problem. The toothpaste composition of the present disclosure essentially excludes the above harmful ingredients.

When brushing teeth using the toothpaste composition of the present disclosure as described above, it is possible to not only remove bacteria in the oral cavity with excellent performance, but also to maintain the antibacterial activity in the oral cavity for a long time compared to the conventional toothpaste.

In the present disclosure, oral bacteria refers to harmful bacteria that are present in the oral cavity of humans and can cause tooth decay and periodontal disease. In the present disclosure, the oral bacteria are Campylobacter rectus, Fusobacterium nucleatum, Porphyromonas gingivalis, Agregati vector actinomysetum Comitans (Aggregatibacter actinomycetemcomitans), Streptococcus mutans, Streptococcus sobrinus, Eikenella corrodens, and Prevotella intermedia. can do.

Here, the Campylobacter rectus causes periodontal sac, periodontal disease, acute periodontal disease, and Fusobacterium nucleatum, Porphyromonas gingivalis, Agregativector actinomysetum comitans, Ikenella coroden S, and Prevotella intermedia are known to cause periodontitis, and Streptococcus mutans and Streptococcus sobrinus are known to cause tooth decay and dental caries.

On the other hand, too frequent brushing may cause abrasion of teeth, which may be rather negative for dental health, and the user has to brush teeth. Therefore, not only the antibacterial activity immediately after brushing, but also the persistence of the antibacterial activity that maintains the antibacterial activity after brushing are important considerations. In the case of conventional toothpastes, it has been shown to have excellent antibacterial activity, but this is only observed for less than 10 hours or less than 15 hours, and there is no suggestion for a toothpaste composition having excellent antibacterial activity even after 24 hours or more.

When applied to oral bacteria, the toothpaste composition of the present disclosure can inhibit the culture of oral bacteria by 90% or more, preferably 95% or more, and more preferably 97% or more.

According to one embodiment of the present disclosure, the toothpaste composition can inhibit the cultivation of oral bacteria by 99% or more when 2 hours elapse after application to the oral bacteria. In the present disclosure, the degree of inhibiting the cultivation of the oral bacteria is expressed as "antibacterial activity", which is "antibacterial activity = OD (Optical Density) / (toothpaste composition) of a mixture of a toothpaste composition and a specific oral bacteria, measured after a certain period of time. The initial OD of + the initial OD of a specific oral bacterium)". Preferably, the antimicrobial activity may be 99.5% or more, more preferably 99.9% or more.

According to an embodiment of the present disclosure, the toothpaste composition may inhibit the cultivation of oral bacteria by 95% or more when 24 hours elapse after being applied to oral bacteria. The toothpaste composition can inhibit the cultivation of oral bacteria by preferably 97% or more, more preferably 97.5% or more, and most preferably 97.9% or more when 24 hours have elapsed after being applied to oral bacteria. Through this, the toothpaste composition may reduce a user's use of toothpaste and reduce excessive brushing frequency.

The present disclosure provides a toothpaste comprising the aforementioned toothpaste composition. The container containing the toothpaste is not particularly limited, and may be provided in a tubular shape, such as a conventional toothpaste.

In the tubular toothpaste, the amount of the toothpaste composition provided to the general consumer is not particularly limited, but according to an embodiment of the present disclosure, the amount of the toothpaste composition in the tube of the tubular toothpaste may be 25g. According to another embodiment of the present disclosure, the amount of the toothpaste composition in the toothpaste tube may be 95g.

Hereinafter, preferred embodiments are presented to aid in the understanding of the present disclosure, but the following examples are provided for easier understanding of the present disclosure, and the present disclosure is not limited thereto.

Example

1. Examples 1 and 2. Preparation of toothpaste

To prepare a toothpaste composition having the component content of Table 2 below. The component content is in units of weight percent. Water was used by mixing purified water and ionic mineral raw water in a volume ratio of 12 to 1, and the composition of the ionic mineral raw water was the same as that of the specific example of Table 1 above.

ingredient Example 1 Example 2 Dental Type Silica 12.0 15 Aminocapronic acid 0.1 Tocopherol Acetate 0.1 0.01 Concentrated glycerin 3.0 D-sorbitol solution 68.0 65.0 Xanthan gum 0.8 0.9 Grapefruit seed extract 0.2 0.2 Matricaria extract 0.05 0.05 Aloe extract 0.05 0.05 Eucalyptus extract 0.05 0.05 Sage extract 0.05 0.05 Calendura extract 0.05 Green tea extract 0.05 Pogongyoung extract 0.01 Propolis extract 0.01 Sodium chloride 0.1 Xylitol 0.2 1.0 Mastic oil 0.005 Spearmint oil 0.05 Fennel oil 0.05 Ascorbic acid 0.01 0.01 Sodium Cocoyl Glutamate 0.2 1.5 Mint flavor additive 0.4 water 18.14 12.505

2. Antibacterial activity test

The antibacterial activity of the toothpaste composition of the present disclosure over time was tested.

First, as Comparative Experimental Example 1, 8 kinds of microorganisms inducing oral diseases derived from human bodies having a Spectrometer optical density (OD) value of about 0.002 were prepared. The microorganisms were plated on each 100 Φ dish and cultured for 2, 4, 6, 8, and 24 hours, and then the culture state of the dish was photographed for each hour, and OD was measured with a spectrometer.

As Experimental Example 1, the toothpaste composition of Example 1 was applied at a concentration of 10 mg/ml to each dish on which each microorganism of Comparative Experimental Example 1 was smeared. The OD value of the toothpaste composition of Example 1 observed at this time was about 2.3636. After incubating the dish to which the toothpaste composition was applied for 2, 4, 6, 8, and 24 hours, the culture state of the dish was photographed for each hour, and OD was measured with a spectrometer.

As Experimental Example 2, an experiment was carried out in the same manner as in Experimental Example 1, except that Example 1 was changed to Example 2. The OD value of the toothpaste composition of Example 2 was about 2.575.

The OD value was measured by a Spectrophotometer (600nm).

The cultured state of the photographed dish is shown in FIGS. 2 to 9. As shown in Fig. 1, each quadrant of the photographed dish of Figs. 2 to 5 represents the culture state of the dish according to the elapse of 2, 4, 6, and 8h. In addition, Figures 6 to 9 show the culture state of the dish 24h.

In addition, the antimicrobial activity of Test Examples 1 and 2 over time is shown in Table 3 below. The antibacterial activity is calculated as dish OD / (initial toothpaste composition OD + initial microbial OD).

After 2 hours Experimental Example 1 Experimental Example 2 Campylobacter retus 99.477 99.447 Streptococcus sorbrinus 99.867 99.940 Eikenella corrodens 99.596 99.533 Streptococcus mutans 99.501 99.456 Prevotella intermedia 99.809 99.906 Porphyomonas gingivalis 99.793 99.507 Aggregatibacter actinomycetemcomitans 99.485 99.472 Fusobacterium nucleatum 99.875 99.917 After 4 hours Experimental Example 1 Experimental Example 2 Campylobacter retus 99.71 99.465 Streptococcus sorbrinus 99.548 99.907 Eikenella corrodens 99.584 99.881 Streptococcus mutans 99.619 99.856 Prevotella intermedia 99.560 99.619 Porphyomonas gingivalis 99.649 99.609 Aggregatibacter actinomycetemcomitans 99.462 99.592 Fusobacterium nucleatum 99.620 99.535 After 6 hours Experimental Example 1 Experimental Example 2 Campylobacter retus 99.478 99.482 Streptococcus sorbrinus 99.509 99.499 Eikenella corrodens 99.763 99.501 Streptococcus mutans 99.678 99.975 Prevotella intermedia 99.918 99.962 Porphyomonas gingivalis 99.614 99.516 Aggregatibacter actinomycetemcomitans 99.458 99.643 Fusobacterium nucleatum 99.840 99.681 After 8 hours Experimental Example 1 Experimental Example 2 Campylobacter retus 99.949 98.424 Streptococcus sorbrinus 98.425 98.316 Eikenella corrodens 98.537 98.451 Streptococcus mutans 98.883 98.752 Prevotella intermedia 98.845 98.826 Porphyomonas gingivalis 98.827 98.381 Aggregatibacter actinomycetemcomitans 98.618 99.008 Fusobacterium nucleatum 98.715 98.569 After 24 hours Experimental Example 1 Experimental Example 2 Campylobacter retus 98.395 98.068 Streptococcus sorbrinus 97.087 97.050 Eikenella corrodens 97.407 97.622 Streptococcus mutans 98.669 98.701 Prevotella intermedia 98.371 98.726 Porphyomonas gingivalis 98.285 98.292 Aggregatibacter actinomycetemcomitans 98.299 98.714 Fusobacterium nucleatum 98.510 98.761

As can be seen through Figures 2 to 9, as a result of observing the microorganisms for 2, 4, 6, 8, and 24 hours, no microorganisms were observed during 2 to 8 hours, but after 24 hours, Comparative Experimental Example 1 In the case of, it was confirmed that there are cases in which microorganisms are observed with the naked eye.

In addition, from the results of Table 3, the antibacterial activity after 2 hours of the toothpaste composition of the present disclosure was measured as an average of 99.9% for each microorganism, and the antibacterial activity after 4 hours and 6 hours was measured as an average of 99.6%, and the antibacterial activity after 8 hours It was measured as 98.7%, and it was confirmed that the antibacterial activity after 24 hours was measured as an average of 97.9%.

3. Oral environment improvement test

(1) Human saliva test

Using the toothpaste consisting of the toothpaste composition of Example 2, brushing was performed for 3 minutes each, 3 minutes a day, 3 times a day for 2 weeks. Saliva was sampled every Friday at 10 am. Ingestion of tobacco, coffee, and beverages was prohibited only prior to sampling of saliva. Saliva was collected using a gargle solution, and the analysis was performed by real-time PCR of Periogen CO., Ltd. Data analysis was performed using ANOVA analysis of variance by STATVIEW software.

The change in the number of microorganisms in the collected saliva was plotted in FIGS. 10 to 12.

(2) Human-derived micro-organism test

Aggregatibacter actinomycetemcomitans (Aa), Streptococcus sobriums (Ss), Streptococcus mutans (Sm) culture solution each 0.2 ml and 0.5 mg / ml of the toothpaste compositions of Examples 1 and 2 diluted in distilled water were reacted for 3 minutes, and then each culture solution was 1 Reincubated for minutes and 5 minutes. Cultures that did not react with Example 1 or 2 were cultured for 1 minute and 5 minutes as a control.

The number and reduction rate of microorganisms measured for each time are shown in FIG. 13. The reduction rate was calculated as (A-B)/(A) * 100, where (A) is the number of microorganisms in the control and (B) is the number of microorganisms in the experimental example.

As shown in FIGS. 10 to 12, a total of 10 types of oral bacteria were detected as a result of the test of (1), and it was confirmed that each oral bacteria decreased for 2 weeks.

In addition, looking at the reduction rate shown in FIG. 13, it can be seen that when the toothpaste composition of the present disclosure is treated, the number of microorganisms in the medium in which human-derived microorganisms are rearranged decreases.

In conclusion, from the test results of (1) and (2) above, it can be seen that the toothpaste composition of the present disclosure has a significant effect on not only temporary reduction of oral bacteria, but also reduction in long-term perspective.

All simple modifications to changes of the present disclosure belong to the scope of the present disclosure, and the specific scope of protection of the present disclosure will be made clear by the appended claims.

Claims (10)

As a toothpaste composition with improved antibacterial performance,
The toothpaste composition is water, wherein the water contains ionic mineral raw material water; abrasive; Humectants; Thickener; Tocopherol acetate, aminocaproic acid, or a combination thereof; And an additive,
The ionic mineral raw material water includes magnesium, silicon, calcium, potassium, and strontium, wherein the ionic mineral raw material water is 1.5 to 4 mg of magnesium and 2 to 5 mg of silicon per 1 L of the ionic mineral raw water, wherein the silicon Is silicon dioxide, which is present in the ionic mineral raw water, and contains 4 to 10 mg of calcium and 0.2 to 1.9 mg of potassium,
The additive is one selected from the group consisting of grapefruit seed extract, matricaria extract, aloe extract, eucalyptus extract, sage extract, xylitol, ascorbic acid, sodium cocoyl glutamate, and combinations thereof,
The toothpaste composition is applied to oral bacteria and the antibacterial performance is improved to inhibit the cultivation of oral bacteria by 95% or more when 24 hours have elapsed. The method according to claim 1,
The toothpaste composition includes 10 to 20% by weight of an abrasive, 60 to 70% by weight of a wetting agent, and 0.5 to 2% by weight of a thickener, based on the total weight of the toothpaste composition,
Wherein the abrasive is dental type silica; The humectant is D-sorbitol liquid, concentrated glycerin, or a combination thereof; Toothpaste composition with improved antibacterial performance, characterized in that the thickener is xanthan gum. The method according to claim 1,
The toothpaste composition further includes other additives, wherein the other additive is one selected from the group consisting of pogongyeong extract, propolis extract, sodium chloride, mastic oil, green tea extract, mint flavor additive, and combinations thereof Toothpaste composition with improved antibacterial performance. The method according to claim 1,
The toothpaste composition further includes other additives, wherein the other additive is one selected from the group consisting of calendula extract, spearmint oil, fennel oil, and combinations thereof. Toothpaste composition with improved antibacterial performance. The method according to claim 1,
The toothpaste composition does not contain harmful ingredients, and the harmful ingredients are fluorine, synthetic surfactants, triclosan, MIT (methylisothiazolinone), CMIT (Methylchloroisothiazolinone), PEG (polyethylene glycol), precipitated calcium carbonate, parabens, saccharin, and tar pigments. Toothpaste composition with improved antibacterial performance comprising a. The method according to claim 1,
The oral bacteria are Campylobacter rectus, Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregati vector actinomycetum comitans. ), Streptococcus mutans, Streptococcus sobrinus, Eikenella corrodens, and Prevotella intermedia. Toothpaste composition with improved antibacterial performance. Toothpaste comprising the toothpaste composition according to any one of claims 1 to 6. delete delete delete

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