KR101221074B1 - Natural anti-bacterial toothpaste composition containing improvement in tooth pain, antibacterial mouth and nerve stability - Google Patents

Abstract

PURPOSE: A natural antibacterial toothpaste composition for children containing water soluble sodium silicate is provided to ensure non-toxicity and non-irritating antibacterial effects, and to tranquilize. CONSTITUTION: A natural antibacterial toothpaste for children with non-toxicity, a non-irritating antibacterial effect, and a tranquilizing effect contains 1-40 wt% of water soluble sodium silicate(Na_2SiO_3-10H_20), 20-25 wt% of water soluble calcium silicate(CaSiO_3), 20-25 wt% of silicon dioxide in a powder form, 10-20 wt% of D-sorbitol of liquid phase, 4-12 wt% of glycerine of liquid phase, 2-8 wt% of corn starch in a powder form, 3-6 wt% of liquid Glycyrrhizae radix, and 1-3 wt% of saccharin in a powder form.

Description

NATURAL ANTI-BACTERIAL TOOTHPASTE COMPOSITION CONTAINING IMPROVEMENT IN TOOTH PAIN ， ANTIBACTERIAL MOUTH AND NERVE STABILITY}

The present invention relates to a toothpaste composition for children, and in particular, water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), water-soluble calcium silicate (CaSiO ₃ ) and herbal medicines containing non-toxic, non-irritating oral antibacterial and natural antibacterial It is safe and harmless even if swallowed, and relates to a non-toxic, non-irritating oral antibacterial and a natural antibacterial children toothpaste composition having a neurological, mental stability effect to have a neurological, mental stability effect.

Toothpaste is a quasi-pharmaceutical product used for brushing the surface of teeth. Especially, toothpaste is mainly used for the purpose of whitening, bactericidal, and interdental cleansing. To this end, toothpaste generally contains ingredients such as abrasives, wetting agents, foaming agents, and sweeteners. It is made to include.

Bad breath is the smell of exhalation through the mouth and generally refers to the smell of people around you. Bad breath is caused by an acquired systemic disease, or by the decomposition of proteins in saliva, food residues, etc., by the microorganisms in the oral cavity, and the resulting amino acids are decomposed by amino enzymes to produce substances causing bad smell. In other words, it is caused by tooth decay or periodontitis as a local factor in the oral cavity. Diabetes and liver function diseases may be caused by oral body diseases, but even in healthy people, bad breath is caused by food residues and bacteria in the oral cavity. Many Koreans who are more likely to be older and more severe during weather conditions, especially those who prefer strong smells like garlic or kimchi, tend to have bad breath and are actively researching ways to effectively reduce it.

In addition, periodontal disease (flavor) is colonized by various bacteria, yeasts and microorganisms cause gingival inflammation and bleeding, the formation of periodontal pockets and destruction of alveolar bone, resulting in damage to the teeth. These periodontal diseases are caused by plaque composed of bacteria that adhere to carbohydrates in the food and adhere to the teeth softly and firmly as moss, and toxic substances which are metabolites of the bacteria, and plaque, which is a substance in which the bacterial membrane is calcified. It progresses without symptoms and sometimes does not cause toothache other than bleeding or bad breath from the gums, and even if the periodontitis is progressing, it is not known. Generally, when the patient feels uncomfortable, the periodontal tissue is already difficult to treat. In many cases, the periodontal disease progresses, the alveolar bone surrounding the root is extensively destroyed and the tooth is lost, and thus the damaged periodontal tissue has a limit in regenerative capacity. do.

In addition, tooth hypersensitivity (such as a symptom of a tooth) may be sensitive when the dentin of exposed teeth is in contact with cold air or irritating food, and may range from mild to intense and persistent pain.

The severity of symptoms depends on the degree of ivory tubules exposed. Teeth can be divided into the enamel of the outer surface (enamel), the dentin immediately below, the dimension of the distribution of nerves and blood vessels, and the gum tissue that protects the teeth. However, if the enamel layer is damaged due to incorrect brushing, gum disease, or tooth decay, or dentin is revealed due to the depression of the gum, external stimulus is transmitted to the tooth nerve through the dentin tubule of dentin, which causes short but intense and intense pain. In particular, the symptom of the symptom is increasing in young people nowadays and treatment is needed from the early stage.

In addition, to prevent dental caries (fluoride) in the concentration of 0.05 ~ 0.1 w / v% is often used in toothpaste or mouthwash. Recently, however, the use of fluoride compounds in toothpaste and mouthwashes can prevent dental caries, but long-term and repeated use of fluoride compounds promotes inflammation in the gums.

Therefore, there is a need for a dentifrice composition capable of suppressing syringe and preventing dental caries without using fluorine.

In accordance with these requirements, Korean Patent Publication Nos. 10-0806050, 10-0815397, and Korean Patent Publication No. 10-2008-0013445 are toothpastes mainly containing sodium silicate having a particle diameter of 1.0 to 2.5 μm in powder form with respect to the total weight of the toothpaste composition. A composition is disclosed.

However, when the above-described sodium silicate aqueous soluble sodium silicate (SiO ₃₎ component is combined with other water or body fluids or blood materials _{Na 2 SiO 3 -5H 2 0,} Na 2 SiO 3 -7H 2 0, ... , Na ₂ SiO ₃ -9 H ₂ 0, Na ₂ SiO ₃ -10 H ₂ 0,. , Na ₂ SiO ₃ -15H ₂ 0, Na ₂ SiO ₃ -16H ₂ 0, Na ₂ SiO ₃ -9H ₂ 0, Na ₂ SiO _3- Except for 10H ₂ 0, there is a problem of being inadequate as a food additive.

In particular, infants and children are weaker in the defense of bacterial forces than adults and are defenseless against contact (kissing, etc.), which is a penetration route, and therefore, natural toothpaste having natural antimicrobial power should be provided.

The present invention is to solve the above problems, including water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), water-soluble calcium silicate (CaSiO ₃ ) and herbal medicines non-toxic, non-irritating oral and natural antibacterial The purpose of the present invention is to provide a safe and harmless to swallow, non-toxic, non-irritating oral antibacterial and natural antibacterial children toothpaste composition having a neurological and mental stabilizing effect.

In addition, the present invention is safe and harmless even if swallowed by non-toxic, non-irritating oral antibacterial and natural antibacterial to the toothpaste composition, non-toxic, non-irritating oral antibacterial and nerve, mental stability effect to have a nerve, mental stability effect It is another object to provide a natural antibacterial children toothpaste composition having.

According to an aspect of the present invention,

Toothpaste composition is characterized in that the water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0) 1 to 40% by weight, water-soluble calcium silicate (CaSiO ₃ ).

Here, the toothpaste composition is 1 to 40% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 20 to 25% by weight of water-soluble calcium silicate (CaSiO ₃ ), silicon dioxide 20 in powder form ~ 25% by weight, liquid D-sorbitol 10-20% by weight, liquid glycerin 4-12% by weight, powder corn starch 2-8% by weight, liquid licorice 3-6% by weight, powder saccharin 1 3 wt%.

According to the present invention, non-toxic, non-irritating oral antibacterial and natural antibacterial children's toothpaste composition having a neuropsychiatric effect, water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0) and water-soluble calcium silicate (CaSiO ₃ ) And herbal medicines can be safe and harmless even when swallowed with non-toxic, non-irritating oral antibacterial and natural antibacterial, and can provide neurological and mental stability.

In addition, according to the present invention, the antibacterial effect of reducing the bacteria in the mouth by the antibacterial activity of the Helicobacter bacteria 11 kinds of bacteria in the oral cavity and the bacteria causing the secondary infection 99.74% of the total 12 species of 99.99 ~ 99.74% antibacterial to cause the plaque The soft properties of the liquid abrasive agent (force) of the inverse trihedral form of SiO ₃ (water-soluble silicon) reduce the wear of the teeth as much as possible and coat the tooth surface for more than 7 hours and at the same time seal the dentinal tubule Subjects can protect their teeth and dentin by preventing ache.

In addition, according to the present invention, semi-permanent sealing of the dentinal tubules can cure the symptoms of ache, and has the characteristics of a safe toothpaste without the irritation of a non-surfactant (SLS), fluorine-free, preservative-free mouth is easy to hurt In addition to dry mouth, the mucous membranes of the oral cavity become thinner and weaker, so it can be used stably for children who are susceptible to small irritations.They can also be used after brushing fruits due to non-surfactant (SLS), fluorine-free and preservative-free. Eating does not feel a change in taste.

1 is a photograph showing the state of the ball bag after 24 hours after administration of the toothpaste composition of the children according to the experimental example of the Syrian hamster (a) is a control, (b) is a treatment tool.
Figure 2 is a photograph (x40) showing the state of the ball bag after 24 hours after administration of the toothpaste composition of the children according to the experimental example of the Syrian hamster (a) is a control, (b) is a treatment It is.
3 is a photograph (x200) showing the tooth surface of a golden hamster fasted for 3 hours.
Figure 4 is a photograph (x200) showing the tooth surface state 1 hour after the administration of the dentifrice composition according to an embodiment of the present invention in a golden sacce of 1 ml each.
5 to 7 are photographs (x200) showing tooth surface states after 2, 3, and 7 hours of administration of a dentifrice composition according to an embodiment of the present invention to a ball bag of a golden hamster, 1 ml each.
8 and 9 are graphs of the tooth surface composition after the administration of the toothpaste composition according to the embodiment of the present invention to the ball bag of the golden hamster 3 hours and 7 hours, respectively, by a surface analysis device.
10 is a photograph showing the teeth of a golden hamster 4 hours after feeding according to an embodiment of the present invention.
11 is a graph showing the component analysis of the tooth surface of the golden hamster 4 hours after feeding according to the embodiment of the present invention.
12 and 13 are photographs before and after ivory capillary sealing of the toothpaste composition according to an embodiment of the present invention.

Hereinafter, described in detail with reference to the accompanying drawings the composition of a non-toxic, non-irritating oral antibacterial and natural, antibacterial children toothpaste composition having a neurological, mental stability effect as follows.

In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions of the present invention, and may be changed according to the intentions or customs of the user, the operator, and the like. Therefore, the definition should be based on the contents throughout this specification.

Natural antibacterial children's toothpaste composition having a nontoxic, non-irritating oral antibacterial and neurological, mental stability effect according to the present invention is 1-40% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), water-soluble silicic acid 20 to 25% by weight of calcium (CaSiO ₃ ), 20 to 25% by weight of silicon dioxide in powder form, 10 to 20% by weight in liquid D-sorbitol, 4 to 12% by weight in liquid glycerin, 2 to 8 in cornstarch in powder form Weight%, liquid licorice 3-6% by weight, powder form of saccharin 1-3% by weight.

Preferably, 17% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 23% by weight of water-soluble calcium silicate (CaSiO ₃ ), 23% by weight of silicon dioxide in powder form, liquid D- 15 wt% of sorbitol, 10 wt% of liquid glycerin, 5 wt% of corn starch in powder form, 4.5 wt% of licorice in liquid form, and 2.5 wt% of saccharin in powder form.

Water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0) is a new material called UMO, which is licensed as food additives, drinking water enhancement additives, etc. to improve the function of toothpaste and enhance the existing UMO It is a water-soluble complex mineral prepared by enhancing the function of. This means that silicon (Si99%), a major component of natural mineral silica, is melted in a liquid state at high heat to be produced in crystal form, and then dissolved in water. Based on this, the toothpaste composition of the present invention further reinforces Si, Ca Na, Ma, Fe and the like to strongly antibacterial to all harmful harmful bacteria in the oral cavity such as teeth and gums, and in particular, SiO ₂ 75 among Na ₂ SiO ₃ -10H ₂ 0. It was developed as a unique toothpaste composition containing more than%.

Soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0) is a product that passed the standard of Article 18, paragraph 2 of the Korean Water Management Act. It contains 63% or more of SiO ₃ and contains natural minerals such as calcium, iron, sodium, and magnesium. It has cell activity, permeability, bactericidal, anti-inflammatory, purifying ability, and neurostable ability.

Water-soluble minerals are one of the five major nutrients along with proteins, fats, carbohydrates, and vitamins that make up the living body, and help maintain immunity by maintaining the appropriate alkaline alkaline PH7.35 ~ 7.45 for normal functioning of human tissues, blood, and cells. Detoxifies various external toxins such as free radicals, metals, soot and environmental hormones that occur in the body, maintains the acidity or basicity necessary for metabolic reactions, controls the balance of water in the body, Perform the function of the catalyst.

Water-soluble silicon is a major component of blood vessels, skeletons, and hair. It is a mineral that supplements mental strength and patience. If various minerals except silicon are important in physical terms, silicon is a mineral that is necessary for mental reasons. Since it is contained in brown rice and barley and is present in the discarded part of the fiber, among others, modern people who use pure white food as a stock tend to be deficient in silicon. Lack of silicon causes aging, forgetfulness, endurance loss, and osteoporosis.

Water-soluble calcium is a mineral that prevents fluid acidification, which is the most important for life support, activates the secretion of insulin, and stabilizes nerves and spirits to promote adaptation to stress. Among the constituents of the body, carbon, hydrogen, and oxygen are the next most, and about 1kg is composed of protein and the ionic state is contained in the body fluid. In particular, water-soluble calcium is essential mineral for pregnant women because it helps the formation of bones and teeth, normal coagulation of blood, absorption of vitamin A, vitamin C, vitamin D and vitamin P, and oxygen formation and breast milk formation.

Water-soluble iron is a mineral that makes blood, and it is an essential element in hemoglobin production that carries oxygen to all organs through the lungs as an essential element in making blood. An iron deficiency leads to anemia, reduced resistance, headaches, paleness and decreased libido.

Soluble sodium is a bactericidal mineral, which regulates the balance of electrolytes, regulates acidity and alkalinity of body fluids, is also involved in the secretion of digestive fluids in the stomach, and is also involved in the infiltration pressure for the entry of nutrients into the blood. Lack of sodium causes heat stroke, vomiting, dyspnea, lethargy, and excessive intake of hypertension, edema, gastrointestinal disease, stomach cancer, heart disease, and atherosclerosis.

Water-soluble magnesium is a mentally stable mineral that stabilizes nerves and mood and improves defense against stress. About 0.02% of body weight, 70% of which is in bones and teeth, catalyzes the activation of enzymes and energy production, and helps to absorb vitamin B, vitamin E, fat and calcium. Lack of magnesium can lead to vasodilation, forgetfulness, convulsive diseases, protein metabolism disorders, arrhythmia, and heart attacks.

The water-soluble calcium silicate (CaSiO ₃ ) is a water-soluble substance, and no deposition or aspiration is detected at all, and is excreted with the substance in the body.

Licorice (Glycyrrhiza uralensis FISCH) is a perennial herbaceous plant belonging to the legume, which is dried and used as a medicinal herb.

Hereinafter, the experimental example of the non-toxic, non-irritating oral antibacterial and natural, antibacterial children's toothpaste composition having a neuropsychiatric effect, as follows. These experiments were commissioned by the Korea Institute of Chemical Convergence Testing.

Single dose toxicity test

17% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 23% by weight of water-soluble calcium silicate (CaSiO ₃ ), 23% by weight of silicon dioxide in powder form, 15% by weight of liquid D-sorbitol Children's toothpaste composition consisting of%, liquid glycerin 10% by weight, powdered corn starch 5% by weight, liquid licorice 4.5% by weight, saccharin 2.5% by weight and prepared a single dose toxicity of the prepared sample In order to do this, 10 male and female rats (control group 5 and administration group 5) were observed for 2 weeks after oral administration of test substance at a rate of 2,000 mg / kg body weight.

1. Test Items

1.1 Sample Classification

Children's toothpaste composition above

1.2 Acute toxicity test

(1) Test system

1) species and lineage; SD rat female and female

2) the reason for the selection of the test system; The SD rats used in this study have been widely used in toxicology tests around the world, so they are rich in basic physiological, anatomical and toxicological data. It also exemplifies the use of rats in the single dose toxicity test of 1999-61.

3) age and weight range; 6-7 weeks old, male 150 ± 10g, female 120 ± 10g

4) Purification and Quarantine; Purified in animal room for about 1 week after animal acquisition. General conditions were observed during the acclimation period and only healthy animals were used for the test.

5) Group Separation and Animal Identification: The weight of healthy animals used in the experiment was measured to separate the groups so that the average weight of each group was almost the same, and the individual identification was the method of marking pigments (picrinic acid) and breeding boxes (Tag). Notation was used.

(2) Breeding environment

1) temperature and humidity ranges; 22 ± 2 ℃, relative humidity 50 ± 10%

2) Contrast Cycle (Cycle); Fluorescent lighting (08:00 lit ~ 20:00 off)

3) roughness; 200 ~ 300Lux

4) types of breeding boxes; Polycarbonate cage for mice during purification, quarantine, administration and observation

5) number of animals per breeding box; Purification, quarantine period-5, dosing period-5

6) feed and drinking water; Rat feed for laboratory animals {Purin Korea Co., Ltd.], drinking water treated with UV sterilization

(3) Composition of test group

The weights of the healthy animals used in the experiment were divided into five groups so that the average weight of each group was almost the same. The individual identification was marked on the tail of the rat, and tag labeling by breeding boxes was used.

(4) Administration method of test substance

1) Recipe

The children's toothpaste composition was applied to test animals by suspending in distilled water.

2) Administration method

Ten female and female rats were fasted overnight, and the sample solution and the test suspension were observed for 14 days after oral administration at a rate of 2,000 mg / kg of body weight.

(5) Observation and Inspection Items

1) general symptoms observation; Changes in general symptoms, intoxication symptoms and the presence of dead animals were observed until 14 days after administration of the test substance.

2) Body weight measurement: Body weight was measured before starting the administration and 7 days after administration.

3) gross organ observation; After 14 days of administration, all animals were necropsied and visually examined for tissue abnormalities. Tissues showing abnormal findings were microscopically examined.

2. Results

(1) General symptoms and mortality rates (Tables 2 and 3)

Observations were observed immediately after the injection and for 14 days after the injection, and no abnormal or dead animals were observed.

Here, N: number of animals examined,-: abnormally undetected.

(2) weight change (Table 4)

As a result of body weight measurement, abnormal weight change was not observed due to the application of the test substance, and both the control group and the administration group showed normal weight gain.

(3) Autopsy Findings (Tables 5 and 6)

At the autopsy of all surviving animals, no gross abnormalities presumed to be due to the administration of this test substance were observed. Table 5 shows males and Table 6 shows females.

3. Conclusion

The test results as described above are as follows.

end. There were no dead animals due to the application of the test substance during the trial.

I. Abnormal symptoms by test substance were not observed in general symptoms.

All. As a result of weight measurement, no weight change was observed due to the application of the test substance.

la. No organs or tissues were observed visually.

Therefore, in the single-dose toxicity test for children's toothpaste composition according to the present invention, when the oral administration of the test substance (2,000 mg / ㎏) by the test substance was no animals showing any abnormal symptoms. Since there were no dead animals in the test animals, the approximate lethal dose was determined to be more than 2,000 mg / kg rat weight. Therefore, in this test, "children's toothpaste composition" is a single dose toxicity test LD ₅₀ is considered to 2,000㎎ / ㎏ body weight (BW).

Oral mucosal stimulation test

17% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 23% by weight of water-soluble calcium silicate (CaSiO ₃ ), 23% by weight of silicon dioxide in powder form, 15% by weight of liquid D-sorbitol %, Liquid glycerin 10% by weight, corn starch in powder form 5% by weight, licorice 4.5% by weight, saccharin 2.5% by weight powder was prepared, and the oral mucosa stimulation for the prepared sample For investigation, 1 ml of test substance was administered to 4 male Syrian hamsters in a ball bag for 5 minutes every hour for 4 hours, and the ball bag was washed with saline. Visual and microscopic examination of the oral mucosa was performed 24 hours after the last administration and washing.

1. Test Items

1.1 Sample Classification

Children's toothpaste composition above

1.2 Oral mucosal stimulation test

(1) Test system

1) species and lineage; Male Syrian Hamster

2) the reason for the selection of the test system; The male Syrian hamster used in this test is widely used in oral mucosal stimulation tests and is illustrated in the oral mucosal test of IS0 10993-10.

3) age and weight range; 7 ~ 8 weeks old male 190 ± 10g

4) Purification and Quarantine; Purified in animal room for about 1 week after animal acquisition. The band was loosely attached to the hamster's neck during the acclimation period to prevent respiratory and feed intake and body weight was observed daily. On the day of the end of the trip, the general condition and oral cavity of the test animals were examined and only healthy animals were used for the test.

(2) Breeding environment

1) temperature and humidity ranges; 22 ± 2 ℃, relative humidity 50 ± 10%

2) Contrast Cycle (Cycle); Fluorescent lighting (08:00 lit ~ 20:00 off)

3) roughness; 200 ~ 300Lux

4) types of breeding boxes; Polycarbonate cage for rats during purification, quarantine, administration and observation

5) number of animals per breeding box; Purification, quarantine period-4, dosing period-4

6) feed and drinking water; Rat feed for laboratory animals {Purin Korea Co., Ltd.], drinking water treated with UV sterilization

(3) Composition of test group

Test material was applied to the right cheek pocket of healthy animals used in the experiment as a treatment, and saline was applied to the left cheek pocket as a control, and the individual identification was indicated on the neck band.

(4) Administration method of test substance

1) Recipe

The children's toothpaste composition was applied to the test animal as it is.

2) Administration method

1 g of the test substance was buried in gauze, and the ball sacs of hamsters were washed with physiological saline and applied to the right sacs. As a control, gauze was soaked in an appropriate amount of saline and then applied to the left cheek pocket. Immediately after application of the test substance, the neckband was worn to prevent the test animal from spitting out the test substance. After 5 minutes of application of the test material, the test material was removed and the ball sacs were washed with physiological saline, and thus, a total of four times were performed once per hour for 4 hours.

(5) Judgment method

1) Visual Evaluation

The test material treatment site and the control site were compared to give a score to each experimental animal based on Table 7 (IS0), and the average value was obtained.

2) Histological Evaluation

The tissue change of the hamster ball bag of the test substance treatment area is given to the scores of individual test animals based on Table 8 (IS0), and the average value is obtained. The stimulation index was calculated based on Table 9 by subtracting the mean of the control site from the mean of the treatment site.

(6) Observation and Inspection Items

1) general symptoms observation; Changes in general symptoms and the presence of toxic symptoms were observed for 24 hours after administration of the test substance.

2) visual observation; 24 hours after dosing, the hamster's ball sacs were examined by visual observation before adding them to the fixative solution (Table 7).

3) microscopic observation; Twenty four hours after administration, the tissue changes in the hamster ball bag were examined and determined based on the microscopic observation table (Table 8).

2. Results

(1) General symptoms (Table 10)

No abnormal symptoms were observed in the test animals for 24 hours after the test substance application.

(2) Visual observation (FIG. 1)

Compared with the control site, no significant gross abnormality was observed in the treated area due to the administration of test substances such as erythema and skin. 1 is a photograph showing the state of the ball bag after 24 hours after administration of the toothpaste composition of the children according to the experimental example of the Syrian hamster (a) is a control, (b) is a treatment tool.

(3) histological observation (Table 11, Fig. 2)

The treatment site of some experimental animals showed only mild edema and vascular hyperemia, and the stimulation index was "0.75", indicating no stimulation. Figure 2 is a photograph (x40) showing the state of the ball bag after 24 hours after administration of the toothpaste composition of the children according to the experimental example of the Syrian hamster (a) is a control, (b) is a treatment It is.

3. Conclusion

In order to investigate the oral mucosal irritation of the children's toothpaste composition, after applying the test substance to the four male Syrian hamsters 5 minutes after removing the test substance, the ball sacs were washed with physiological saline, and thus for 1 hour for 4 hours Four times each one was performed. The general symptoms and local irritation at 24 hours were assessed for 24 hours after 4 treatments.

No abnormal findings due to the application of the test substance were observed in the general symptoms during the study. After the exposure of the test substance, no visible observation and irritation such as erythema and skin were observed in the applied area.Only microscopic observation of the oral tissue showed only slight edema and vascular hyperemia at the site of application of the test substance. The index was evaluated as "0.75". Therefore, the stimulus index is 0.75, which is considered to be nonirritating.

In view of the above results, in the oral mucosal test of the children's toothpaste composition according to the present invention in the Syrian hamster, it is judged to be a non-irritating substance.

<< antibacterial experiment >>

17% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 23% by weight of water-soluble calcium silicate (CaSiO ₃ ), 23% by weight of silicon dioxide in powder form, 15% by weight of liquid D-sorbitol %, Liquid glycerin 10% by weight, powdered corn starch 5% by weight, licorice 4.5% by weight, saccharin 2.5% by weight powder was prepared, and the antimicrobial activity of the prepared samples Evaluated.

Test strains include Streptococcus mutans, Streptococcus Mitis, Streptococcus salivarius, Actinomyces sp. And Neisseria sp. .), Bacteroides vulgatus, Veillonella sp., Rothia sp., Fusobacterium nucleatum, Norcardia sp.), Corynebacterium sp., and Helicobacter pylori were tested by the KS K0693 method.

Prepare 0.4g of each specimen and control specimen, place the specimen in 30ml glass container with screw lid, take 0.2ml of enriched test strain, and spread it evenly on the specimen and control piece in each glass container. Carefully inoculated. After inoculation, the lid was closed and incubated for 18 hours at 37 ± 1 ° C., and the number of viable cells was counted and the percentage reduction was calculated. The reduction rate of the test plot for the control was calculated by the following equation.

Here, A is the number of bacteria in the control, B is the number of bacteria in the test.

The test results are shown in Table 12 below.

In the periodontology department of the Seoul National University Dental College, the above 12 bacteria are strains that cause tooth decay (dental caries) and gum flavor (periodontal disease), which are representative diseases of the oral disease among the oral diseases, and the induced diseases are defined in Table 13 below. It was.

As shown in the above results, the toothpaste composition according to the present invention is weakened by antibacterial to 99.95% to 99.99% of various oral fungi in the oral cavity including Streptococcus mutans, which is a representative bacterium of caries. It is effective in improving teeth by preventive treatments such as tooth decay, tooth pain and acute infection, and alleviating and preventing pain in the oral cavity by antibacterial (antibacterial activity 99.54 ~ 99.99%) of 12 kinds of bacteria causing intervening diseases of oral disease, gum disease (perfection) It can be used to treat and remove bad breath. It is effective for nerve stabilization such as nerve stabilization and stress relief by absorbing oral water such as silicon and minerals of water-soluble sodium silicate.

Helicobacter pylori, which is known to cause gastric cancer and gastric ulcers, is the cause of bad breath and periodontal disease. Recent studies have reported that Helicobacter pylori causes problems in the oral cavity rather than the stomach or intestine, which are known as major habitats.

A research team at the Fukuoka Dental University in Japan has detected the DNA of Helicobacter pylori in the mouth of a person with a bad breath without gastrointestinal disease, the latest issue of the British Medical Microbiology. The team surveyed 251 people with bad breath and found that those who detected Helicobacter pylori had more swaying teeth, a greater gap between their teeth and gums, and more blood leaking than those who did not.

According to a paper published by the University of Buenos Aires, Argentina, published in 2005 by Helicobacter, 40 out of 46 people (87%) who had a bad breath without gastrointestinal tract or systemic disease were found in the mouth of Helicobacter pylori.

Hong Chung-pyo, professor of intraoral medicine at Kyung Hee University Dental Hospital, said, "Tooth shaking and deep periodontal pockets between teeth and gums mean that the gums are inflamed." "When the gums are inflamed and blood bleeds out, the bacteria in the oral cavity can rot the blood cells in the blood and cause a bad breath," he said.

Seol Sang-young (Vice Chairman of the Helicobacter and Upper Gastrointestinal Research Group), Pusan Paik Hospital, said, "Helicobacter pylori is known to live the most in the stomach, but sometimes comes along the esophagus into the oral cavity." In addition, patients with gastroesophageal reflux or vomiting may have Helicobacter pylori bacteria mixed into the gastric juice and into the mouth. However, the bacteria are less active in the oral cavity, which has different stomach and acidity, Seol said.

Kim Kap-kyun, a professor of Oral Microbiology at the Seoul National University Dental Hospital, said, "Helicobacter pylori can be in the oral cavity, but the bacteria must gather and metabolize quite a lot to cause bad breath or periodontal disease."

Teeth surface coating experiment

17% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 23% by weight of water-soluble calcium silicate (CaSiO ₃ ), 23% by weight of silicon dioxide in powder form, 15% by weight of liquid D-sorbitol Children's toothpaste composition consisting of%, 1% glycerin 10% by weight, 5% by weight of corn starch in powder form, 4.5% by weight licorice in liquor, 2.5% by weight saccharin in powder form, and the tooth surface coating effect of the prepared sample Was tested.

Golden hamster was used to investigate the tooth coating (film) formation of the toothpaste composition prepared as described above. Hamsters have a ball sac (sclera) that can store feed in the oral cavity so that toothpaste can be exposed to hamster teeth for a period of time by administering the toothpaste to the ball sac.

1 ml of the toothpaste composition according to the present invention was put into a ball bag of a golden hamster fasted for 3 hours, and extracted after 1, 2, 3, and 7 hours, and then coated on the surface of the tooth with an electron microscope (Scanning Electron Microscope, SEM). Formation was examined.

3 is a photograph (x200) showing the tooth surface of a golden hamster fasted for 3 hours.

Referring to Figure 3, it was confirmed that no coating on the tooth surface of the golden hamster before the toothpaste treatment.

Figure 4 is a photograph (x200) showing the tooth surface state 1 hour after the administration of the dentifrice composition according to an embodiment of the present invention in a golden sacce of 1 ml each.

Referring to FIG. 4, it was confirmed that the tooth surface of the golden hamster was coated (coated).

5 to 7 are photographs (x200) showing tooth surface states after 2, 3, and 7 hours of administration of a toothpaste composition according to an embodiment of the present invention to a ball bag of a golden hamster, 1 ml each.

5 to 7, it can be seen that the coating (film) is maintained on the tooth surface of the golden hamster.

Next, 1 ml of the toothpaste composition according to the present invention was administered to the ball bag of the hamster, and the components of the hamster's tooth surface after a predetermined time were analyzed using an energy despersive X-ray analysis system (EDX).

8 and 9 are graphs of the tooth surface composition after the administration of the toothpaste composition according to the embodiment of the present invention to the ball bag of the golden hamster 3 hours and 7 hours, respectively, by a surface analysis device.

8 and 9, it was confirmed that the silicon (Si) coated on the tooth surface appears.

Next, drinking water was provided to empty the golden hamster's ball sac as an experimental pretreatment process, and 1 ml of toothpaste composition was administered to the ball sac of the golden hamster without feeding for 5 hours, and the feed was fed for 3 hours after 3 hours. . After feeding, the golden hamster's teeth were extracted, and the level of coating (film) retention on the tooth surface was examined by electron microscope, and the surface analysis device (EDX) was used for component analysis.

10 is a picture showing the teeth of the golden hamster 4 hours after feeding according to an embodiment of the present invention, Figure 11 is a component analysis of the tooth surface of the golden hamster after 4 hours after feeding according to an embodiment of the present invention It is a graph.

10 and 11, the coating is maintained on the tooth surface even after 4 hours after feeding, it was confirmed that the silicon (Si) component is detected on the tooth surface.

As a result of the test, the toothpaste composition according to the embodiment of the present invention is a water-soluble toothpaste composition to form a coating on the tooth surface for more than 7 hours, the tooth forming effect is to protect the teeth and have a smooth effect for a long time.

<< ivory customs sealing experiment >>

17% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 23% by weight of water-soluble calcium silicate (CaSiO ₃ ), 23% by weight of silicon dioxide in powder form, 15% by weight of liquid D-sorbitol %, Liquid glycerin 10% by weight, powdery corn starch 5% by weight, licorice 4.5% by weight, powdery saccharin 2.5% by weight to prepare a toothpaste composition of the prepared sample, Experiment.

12 and 13 are photographs before and after ivory capillary sealing of the toothpaste composition according to an embodiment of the present invention.

12 and 13, the result of using the toothpaste of the present invention in the exposed ivory tubules was confirmed that the ivory tubules are sealed after 3 weeks. In other words, the dentinal tubules, which are the cause of ache, are blocked and the symptom of ache is alleviated.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It is to be understood, however, that the invention is not to be limited to the specific forms thereof, which are to be considered as being limited to the specific embodiments, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims. .

Claims (2)

1-40% by weight of water-soluble sodium silicate (Na ₂ SiO ₃ -10H ₂ 0), 20-25% by weight of water-soluble calcium silicate (CaSiO ₃ ), 20-25% by weight of silicon dioxide in powder form, liquid D-sorbitol of 10-20% by weight, liquid glycerin 4-12% by weight, powdered corn starch 2-8% by weight, liquid licorice 3-6% by weight, powder form saccharin 1-3% by weight Natural antibacterial children's toothpaste composition having nontoxic, non-irritating oral antibacterial and nerve, mental stability. delete

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