KR102180886B1 - Extract containing roots fermented and whole body of ginseng cultivated in cold wind and cosmetic composition containing the same - Google Patents

Abstract

The present invention provides ginseng cultivated in cold wind. Disclosed are: a fermented plant extract of ginseng cultivated in cold wind, which uses the whole plant; a novel cosmetic composition having excellent anti-oxidant, anti-aging, and anti-wrinkle effects and including the fermented plant extract as an active ingredient; and a food composition.

Description

{Extract containing roots fermented and whole body of ginseng cultivated in cold wind and cosmetic composition containing the same}

The present invention relates to an outpost extract containing a root fermented product of 3-year-old cold wind cultivated ginseng and a cosmetic composition comprising the same as an active ingredient.

Korean ginseng (Panax Ginseng C.A. Meyer) has 43 kinds of ginsenosides, has a high content and a balanced content ratio, and as a result, its high-potency pharmacological effect is recognized. The composition of Korean ginseng is 3-6% ginsenoside, 0.05% vitamin, 1-2% fat, 4-6% newsletter, 12-16% nitrogen-containing compounds, and 60-70% carbohydrates, whereas American ginseng (Hwagisam) , In the case of Chinese ginseng (samchil ginseng) and Japanese ginseng (jukjeolsam), there are only 19 types, 29 types and 8 types of ginsenosides that have pharmacological effects. This has a great effect on the climate and soil quality according to the distribution region. It is evaluated as a North American region between 33.7 to 43.1 degrees north latitude and 85 to 140 degrees east longitude and 70 to 97 degrees east longitude. .

Ginseng is a medicinal herb plant that strengthens the function of the human body's five organs. Among Heo Jun's 4010 prescriptions for Donguibogam, ginseng as a raw material accounts for 18.5%.

The pharmacological efficacy of ginseng improves the spirit of the five intestines, stabilizes the mind, opens the heart, improves memory, and reinforces damaged organs (Jun Heo, Dong Dong Bogam 1610).

Ginseng is known as a staple medicine among the staple medicines that have only a rejuvenating effect and no side effects even when taken for a long period of time (China, Dohonggyeong Shinnongbonchogyeongpyeon). In addition, according to Donguibogam above, it opens the hole in the heart and replenishes energy from the disease accumulated by fatigue to stabilize the mind and body and brighten the eyes, and ginseng was first used by domestic and foreign scholars to increase the effect of a flu (influenza) vaccine and improve liver disease. In particular, it has announced the efficacy of improving the weak constitution and enhancing the immunity of the human body.

In Chinese medicine, the ginseng chilhyo theory (人蔘七效說) recorded in Chinese medicine is reinforced, remedy for collapse, conscience anshin, bopungjeongcheon (reinforces lung function and stops coughing), removes toxins in the body, and removes toxins in the body, soothes the skin from inflammation. Chorus), increased blood production and mycorrhizal veins (one-blooded veins), supplementing the essence and quenching thirst (Saengjinjigal), and gastrointestinal strength and stopping diarrhea (Geonbijisa).

According to the first research results, saponin is known as a substance called ginsenosides as a physiologically active substance of glycosides present in the leaves, stems, seeds, shells and roots of ginseng plants, and promotes immunity, anti-inflammatory, anti-allergic, and anti-cancer. , Blood pressure lowering, anti-cholesterol, anti-thrombosis, anti-aging, etc.

Korean Patent Application No. 10-2017-0146206 discloses a ginseng leaf fermented extract for skin whitening with enhanced ginsenosides Rh1, Rh2 and Rh3 content, and a composition for treating diabetes using an extract fermented ginseng with Pediococcus pentosaceus strain It is disclosed in patent application 10-2009-0115334.

In addition, the cosmetic composition for improving skin wrinkles by inhibiting the collagen degradation enzyme of fermented ginseng extract using Lactobacillus casei strain is applied for patent application No. 10-2016-0095756, and the cosmetic composition for skin whitening and anti-inflammatory function is applied for patent No. 10-2016-0002796 In addition, antioxidant, anti-allergic, whitening and skin wrinkle-improving functional cosmetic compositions using ginseng sprout fermented extract as an active ingredient have been disclosed in Patent Application No. 10-2015-0113822, respectively.

In addition, ginseng root is inoculated with Pichia ciferrii strain to increase the content of ginsenosides Rg3, Rh1 or Rh2 by inoculating ginseng root with Lactobacillus paracasei strain in patent application 10-2012-0111222 for skin moisturizing and anti-aging functional cosmetic composition. The composition has been known as Patent Application No. 10-2015-0172634, and a patent application for an external application for suppressing atopic dermatitis disease inhibits the activities of MCP-1, IL-8 and IL-6 by inoculating Lactobacillus plantarum strain into ginseng root. It has been known from 10-2012-0045101.

In addition, a case of providing an antioxidant functional cosmetic composition by inoculating and fermenting 72 strains of Paenibacillus genus strain RMKS into ginseng root was converted to ginsenoside RbI->Rd in Patent Application No. 10-2015-0091329, Trichoderma reesei strain The inoculated and fermented ginseng root fermentation extract contains high ginsenoside Rd, Rg3-R, and Rk1, so patent application No. 10-2011-0065605, and Lactobacillus fermentum strain was inoculated into ginseng root to inoculate Rg3, Compound-K, Fermented products containing F1 are known in patent application 10-2008-0009331. Furthermore, Korean Patent Application Laid-Open No. 10-2015-0155911 discloses a method of increasing antioxidant quality by fermenting ginseng fruit extract.

The above published or registered patent documents are technologies for bioconversion of various ginsenosides by inoculating ginseng root, sometimes ginseng leaves or ginseng fruit, obtained from field cultivation or hydroponic cultivation into lactic acid bacteria or yeast.

However, it is an object of the present invention to provide an outpost extract comprising a fermented broth of lactic acid bacteria of the root of a novel cold product cultivated ginseng with improved ginsenoside content.

Another object of the present invention is to provide a cosmetic composition capable of improving skin absorption rate by enhancing the content of low-molecularized amino acids containing the lactic acid bacteria fermented extract of the cold air cultivated ginseng.

Another object of the present invention is to provide a method for producing a fermented extract of the ginseng outpost lactic acid bacteria.

The above object of the present invention is that ginseng plants photosynthesize only for about 5 months from May to September of the year, and the remaining period is only in the underground part, and in consideration of the characteristic of respiration for a year, an average of 12 to 14 per month. Provides a method of early harvesting 6-year-old ginseng through a pesticide-free cultivation method for 3 years by maintaining an optimum temperature of 20~25℃, preferably 22℃, even in the summer by blowing cold air into an underground cave where ℃ is maintained, a green house or a concrete room. And (a);

Among the whole body ginseng, such as the green cultivated ginseng root and the above-ground parts of leaves, stems, fruits and flowers, the ginseng root obtained in the above step is fermented with Lactobacillus plantarum, an intestinal microbial lactic acid bacteria that helps digestion in the large intestine. Obtaining step (b);

The above-ground part (leaves, stems and flowers) is closed in a medicinal cloth to extract hot water at 60° C. for 24 hours in a medicine bath (c);

The ginseng root fermented extract obtained in steps (b) and (c) and the above-ground hot water extract are most preferably blended in a ratio of 1:0.4 (w/w) to prepare a cosmetic composition using the ginseng outpost fermented extract as an active ingredient. It is achieved by evaluating function.

The ginseng outpost fermented extract of the present invention has the effect of moisturizing and enhancing skin elasticity by reducing the expression of the wrinkle generating factor MMP-1, preventing or treating pigmentation such as spots and freckles, exhibiting skin whitening effect, reducing atopic dermatitis and itching, and It has an excellent effect showing antibacterial effect against acne bacteria.

1 is a photograph showing a cold wind temperature (annual average) of 20 to 25°C in a green house for cultivating ginseng cold products using cold wind of an abandoned mine according to the present invention.
Figure 2 is a photograph showing the present invention ginseng cold wind eco-friendly cultivation house.
3 is a graph showing the bioconversion effect by the lactic acid strain of Compound K derived from ginseng grown in cold wind of the present invention.
4 is a schematic diagram showing the basic concept of the present invention.
5 is a graph showing the skin protection (photoaging) effect of 90% or more from UV-B when 2% (v/v) of ginseng outpost fermented extract (Ginactive Ferm) according to the present invention is treated on skin cells.
6 is a graph showing an increase in collagen of 20% or more when skin cells are treated with 1% (v/v) Ginactive Ferm of the present invention.
7 is a graph showing the antioxidant effect of about 23% and 6.3% (v/v) treatment with Ginactive Ferm 0.4% (v/v) of the present invention, and 87% or more.
8 is a graph showing the maximum anti-inflammatory effect of 53.3% when treated with 0.45% (v/v) Ginactive Ferm of the present invention.
9 is a treatment process diagram of the present invention cold wind cultivated ginseng outpost.

According to the present invention, the present invention ginseng fermented extract (Ginactive Ferm) is characterized by using ginseng whole body grown in cold air medium temperature. The optimum temperature for photosynthesis of ginseng is 20 to 30°C, most preferably 25°C. At temperatures below the minimum, the amount of photosynthesis is lowered, and above 25°C, the amount of respiration is greater than the amount of photosynthesis and the growth rate is slowed.

In addition, according to the present invention, the ginseng fermented extract of the present invention is a cosmetic composition containing as an active ingredient a 1:0.4 mixture (Ginactive Ferm) of lactic acid bacteria fermented product of ginseng root and hot water extract of leaves, stems, flowers, and fruits in the underground part of ginseng. It characterized in that it provides.

In the ginseng obtained as a result of eco-friendly pesticide-free cultivation according to the ginseng cold cultivation method of the present invention, heavy metal contamination and residual pesticides were not detected (Table 1).

The present invention of ginseng outpost fermented extract (Ginactive Ferm) prepared according to the embodiment of 3-year-old (about 1000 days cultivation period) outpost ginseng and outdoor cultivation ginseng obtained as a result of the cold wind cultivation method of ginseng according to FIGS. 1 and 2 Table 2 shows a comparison of the contents of each type of ginsenoside.

As shown in Table 1 and Table 2 above, as shown in Table 1 and Table 2 above, ginsenosides Rb3, F2, Rg3, which are free of heavy metal contamination and pesticide residues, and do not exist in 6-year-old ginseng in the open field, are contained, and the components of Re, Rg1, and F1 are about twice as much. There is a characteristic contained above. In particular, when inoculating and fermenting a strain of the genus Lactobacillus to a three-year-old cold wind cultivated ginseng root, it was possible to confirm the bioconversion that can maximize the Compound K content (FIG. 3).

A cosmetic composition comprising an outpost extract containing a root fermented product of cold-air cultivated ginseng may contain conventional auxiliary agents and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, in addition to the above extract, and carrier Can be formulated with.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing It may be formulated as a cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. More specifically, flexible lotion, nutrient lotion, nutrition cream, massage cream, essence, eye cream, BB cream, CC cream, hair tonic, shampoo, conditioner, cleansing cream, cleansing foam, cleansing water, pack, spray and powder It can be prepared in a formulation.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

In addition, the cosmetic composition containing the root fermented product of cold-air cultivated ginseng of the present invention, preferably the outpost extract liposome as an active ingredient, is very safe compared to the conventional chemical cosmetic, so that the cosmetic composition for enhancing skin elasticity or for preventing aging It is expected that it can be used very usefully.

Cold wind cultivation of ginseng

In accordance with the present invention, as shown in Fig. 1, ginseng is sown in a green house using cold air from an abandoned mine, and the basic concept of the invention of the ginseng outpost fermented extract using ginseng outpost grown at a temperature of 20 to 25°C for 3 years is illustrated in FIG. As shown in.

Efficacy of fermented extract of ginseng outpost grown in cold air

A treatment process diagram for obtaining fermented ginseng outpost fermented extract obtained according to the basic concept according to FIG. 4 using an abandoned mine is shown in FIG.

(1) washing

Clean the roots, leaves, stems, flowers, and fruits of Naengpumsam.

(2) Natural drying and storage

Under the sky above 60℃, dry naturally (solar drying), collect the above-ground part and vacuum-pack it, and vacuum-pack the underground ginseng root separately and store it.

(3) above-ground extraction

Use Propanediol as a solvent, mix with the above-ground part so that it is 1:100 (v/v), extract at 60℃ for 24 hours, store at room temperature, and use it as a test material.

(4) fermentation of the underground part

The roots of cold-pung ginseng and distilled water are mixed at a ratio of 1:10 (w/w), extracted with hot water for 2 hours at 60°C, and sterilized for 121°C/20 minutes. Move to a sterile field, inoculate 1% (v/v) of Lactobacillus plantarum strain, incubate at 37°C for 16-20 hours, kill live bacteria at 121°C/20 minutes, and store it, and use it as a public material.

(5) Mixing of above-ground extract and underground fermentation

As a result of a number of experiments, the most preferable mixing ratio of the underground fermentation extract and the above-ground hot water extract was 1:0.4 (v/v) to maximize the compound K content and other Rg3, Rb3, F1, F2, Re, and Rg1 content. Hereinafter, the final product name according to the present invention was named Ginactive Ferm, and the efficacy test was performed.

Efficacy of Ginseng Outpost Fermented Extract (Ginactive Ferm) Grown in Cold Wind

[Experimental Example 1] MMP-1 mRNA expression

Experimental results of the present inventors of the present invention are the characteristics of the "Ginactive Ferm" mixture of the present invention. The representative ginsenosides of the hot water extract of the cold wind cultivated ginseng are F1, F2, and the representative ginsenosides of the fermented extract of the L. plantarum strain are compound K and the basement part. In consideration of the fact that it is Rg3, an MMP-1 mRNA expression experiment was conducted. The experimental results were shown in FIG. 5. It was found that the increase in the oxidative stress of skin cells by UV-B accelerates the expression of matrix metalloprotease (MMP) and suppresses skin aging due to skin collagen reduction. As a result of treating fibroblasts with 2% Ginactive Ferm, more than 90% of the skin protection effect was shown, so it was confirmed that there is an effect of improving wrinkles.

[Experimental Example 2] Collagen enhancement effect

According to the experimental results of the present inventors, it was confirmed that procollagen type 1 was increased in fibroblasts in invitoro. As shown in FIG. 6, when 1% of Ginactive Ferm was applied to the skin cells, a cosmetic composition having an anti-wrinkle effect could be obtained by confirming a collagen enhancing effect of about 20%.

[Experimental Example 3] DPPH scavenging activity

As a result of the experiments of the present inventors' DPPH scavenging activity, a cosmetic composition having an antioxidant effect could be obtained by confirming the maximum 87% antioxidant effect in the 6.3% treatment of Ginactive Ferm. (Fig. 7).

[Experimental Example 4] Anti-inflammatory effect

As a result of the experiment of the present inventors' anti-inflammatory signal transducer, Nitric oxide (nitrite) scavenging ability, a cosmetic composition having an anti-inflammatory effect could be obtained by confirming an anti-inflammatory effect of up to 53.3% in 0.45% Ginactive Ferm treatment. (Fig. 8).

Hereinafter, preferred and various cosmetic formulations for using the present invention are disclosed, but the scope of the present invention is not limited to the formulation disclosed in the specification of the present invention.

[Production Example 1]

toilet water

The lotion containing the outpost extract containing the root fermented product of the cold wind cultivated ginseng obtained in Example 4 was prepared as shown in Table 3 below.

Raw material Content (% by weight) One Outpost extract containing fermented roots of ginseng grown in cold wind 10.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hydrogenated castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance

[Production Example 2]

Lotion

A lotion containing the outpost extract containing the root fermented product of the cold air cultivated ginseng obtained in Example 4 was prepared as shown in Table 4 below.

Raw material Content (% by weight) One Outpost extract containing fermented roots of ginseng grown in cold wind 10.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solbidan Sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbidan Stearate 1.0 7 Subtype monostearinsine glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate/Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxypolymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

[Production Example 3]

cream

The cream containing the outpost extract containing the root fermented product of the cold air cultivated ginseng obtained in Example 4 was prepared as shown in Table 5 below.

Raw material Content (% by weight) One Outpost extract containing fermented roots of ginseng grown in cold wind 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene Sorbidanmosostearate 0.5 6 Solbidansesquioleate 0.5 7 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Floating paraffins 4.0 10 Squalane 4.0 11 Caprylic/Capric Triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

[Production Example 4]

essence

The essence containing the outpost extract containing the root fermented product of the cold air cultivated ginseng of the present application obtained in Example 4 was prepared as shown in Table 5 below.

Raw material Content (% by weight) One Outpost extract containing fermented roots of ginseng grown in cold wind 10.0 2 Cytosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

From the above description, those skilled in the art to which the present application belongs will understand that the present application may be implemented in other specific forms without changing the technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present application should be construed as including all changes or modified forms derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof.

The present invention provides a cold wind cultivated outpost ginseng to provide its fermented extract (Ginactive Ferm) and at the same time provides an antioxidant, whitening and anti-wrinkle, and anti-inflammatory functional cosmetics containing it, so it is a very useful inventor in the cosmetics and health food industry. will be.

Claims (5)

A mixture of extracts obtained by hot-water extraction of the above-ground part of cold-air ginseng obtained by introducing cold air from an abandoned mine into a green house and rapidly cultivating it at 20~25℃ for 3 years and fermented extract of lactic acid bacteria in the underground part (Ginactive Ferm) The cosmetic composition according to claim 1, wherein the mixture (Ginactive Ferm) contains a mixture of 1:0.4 (v/v) of the hot water extract of the above-ground part of cold-air ginseng and the fermented extract of lactic acid bacteria in the basement part as an active ingredient. Health functional supplement composition containing the mixture of claim 1 (Ginactive Ferm) as an active ingredient Antioxidant, whitening, anti-inflammatory or anti-wrinkle cosmetic composition containing the mixture of claim 1 (Ginactive Ferm) The cosmetic composition according to claim 4, wherein the cosmetic composition is any one formulation selected from lotion, lotion, cream, and essence.

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