KR101690144B1 - A method of preparing cosmetic composition for moisturizing the skin and promoting the differenciation of keratinocyte cell comprising the agar and the cosmetic composition prepared therefrom - Google Patents

Abstract

The present invention relates to a method for producing a cosmetic composition for promoting skin moisturization and dermal keratinocyte differentiation comprising agar as an active ingredient and a cosmetic composition prepared thereby, The cosmetic composition for moisturizing the skin containing the agar as an active ingredient is effective not only for moisturizing the skin but also for promoting the differentiation of dermal keratinocytes and restoring the skin barrier function. The cosmetic composition according to the present invention contains agar as an active ingredient to promote differentiation of keratinocyte, restores skin barrier function, and relieves tension and roughness of the skin and increases elasticity. Thus, cosmetic composition for skin moisturizing Can be usefully used.

Description

[0001] The present invention relates to a method for preparing a cosmetic composition for promoting skin moisturization and skin keratinocyte differentiation promoting the skin containing agar as an active ingredient and a cosmetic composition prepared thereby and a cosmetic composition prepared thereby. and the cosmetic composition prepared therefrom}

The present invention relates to a method for producing a cosmetic composition for skin moisturizing using the gelation property of agar and a cosmetic composition prepared thereby. More specifically, the present invention relates to a cosmetic composition for skin moisturizing, , A method for producing a cosmetic composition having an effect of promoting skin moisturization, differentiation of dermal keratinocyte differentiation promoting skin barrier strengthening function, and a cosmetic composition prepared thereby.

Skin plays a very important role as a barrier function to protect individuals from the outside. The barrier function is a protection function for protecting against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and excessive divergence of water in the body through the skin. Such protection function is a function of protecting the stratum corneum The function can be maintained only when it is normally formed.

The outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes, and consists of keratinocytes with complete differentiation and a surrounding lipid layer (Marcelo CL et al., J. Invest. Dermatol. 80, pp 37-44, 1983).

The keratinocyte is a characteristic cell in which basal cells continuously proliferating in the lowest epidermis undergo a stepwise change in morphology and function and are elevated to the surface of the skin. After a certain period of time, And the new keratinocyte replaces its function. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization".

In addition, during keratinization, keratinocytes form natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids) and form stratum corneum, which gives firmness and flexibility to the stratum corneum, barrier function.

Such stratum corneum can easily lose its function due to environmental factors such as excessive washing, bathing and other lifestyle factors, dry air pollutants, and endogenous diseases such as atopic skin and senile skin. In fact, Due to the increasing number of factors, dry skin symptoms and their complaints have been increasing recently.

Therefore, in order to maintain proper skin moisture, various studies have been made to supply water from the outside or to prevent water loss from the body. Various moisturizers having moisture retention ability have been developed. As a skin moisturizing agent, it is common to use a substance capable of increasing water retention in the stratum corneum such as ceramide and essential fatty acid and lipid complex (Rawlings AV et al., J. Invest. Dermatol., 5, pp731- 741, 1994).

However, recently, the risk factors for skin are increasing, and changes in dietary patterns slow down the generation and decline of the stratum corneum, and the function of the keratinocyte is reduced, so that the amount of the moisturizing factor and lipid in the stratum corneum is decreased, There is an increasing trend in people with skin that can not function as a skin barrier. Such breakdown of the skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis and psoriasis. Such diseases can be expected to be alleviated by moisturizing agents having a conventional moisture retention function but it is difficult to achieve fundamental healing.

As cosmetic compositions for promoting skin moisturization, emulsion, lotion, cream, simple oil dispersion type, and high viscosity emulsion type formulations using a large amount of wax have been conventionally used.

In this field, agar is used to make cushion and tension feeling on the surface by hardening the surface of the preparation by utilizing the gelling properties of agar, unlike the formulations generally used in the past, and by the gel ringing in mesh form, The present invention is directed to a cosmetic preparation method for imparting an effect of increasing skin moisturizing power by effectively transferring it to the skin.

On the other hand, agar is a white to light yellowish white translucent product which is dried by freezing and dehydrating mucus from the same genus or other kinds of red algae. This agar absorbs moisture from the intestines and has the property that the volume of the agar itself blows, thereby increasing the amount of feces in the intestines. The nature of the agar fiber, which is not absorbed in the intestines, causes harmful substances such as sugars, By blocking the gastrointestinal wall so that it can be contained in the feces, it has the effect of preventing constipation, preventing overeating by increasing the filling feeling, and preventing obesity.

And agar absorbs moisture, heavy metals and fat in a liquid state in the stomach, and by solidifying it to minute change it prevents absorption of high fat content and converts it to low fat, thereby preventing diabetes, hyperlipidemia, prevention of liver function decrease, And the like.

In addition, agar prevents obesity, hypertension, hyperlipidemia, hyperglycemia, and other four malignant diseases, reducing the incidence of arteriosclerosis to about 40 times. In addition, carrageenan (a complex polysaccharide core), which is contained in agar, is an undigested part of the fiber, which reduces excessive activity of the pancreas and reduces overproduction of insulin, thereby preventing gallstones, preventing cancer of the intestines, have.

However, there has been no report on the effect of agar skin moisturizing or skin barrier enhancement.

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a method for producing a cosmetic composition that is superior in moisturizing and moisturizing using agar.

Another object of the present invention is to provide a cosmetic composition using agar prepared by the above-described method.

In order to accomplish the above object, the present invention provides a method for preparing a cosmetic composition for promoting skin moisturization and dermal keratinocyte differentiation, which comprises agar as an active ingredient.

In addition, the present invention provides a cosmetic composition for promoting skin moisturization and dermal keratinocyte differentiation, which comprises agar prepared by the above production method as an active ingredient.

According to the present invention, a cosmetic composition for skin moisturizing, which comprises agar prepared by using the agar-like gelation property of agar as an active ingredient, is effective not only for skin moisturization but also for promoting differentiation of dermal keratinocyte and restoration of skin barrier function. The cosmetic composition according to the present invention has the effect of eliminating the pulling and roughness of the skin and increasing the elasticity, and thus can be usefully used as a cosmetic for moisturizing the skin.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram illustrating a process for producing a cosmetic composition containing agar of the present invention as an active ingredient. FIG.

The present invention provides a method for producing a cosmetic composition comprising agar as an active ingredient, which is characterized by utilizing the property of gelling in the form of a mesh of agar. Preferably, the method of making a cosmetic composition of the present invention is characterized by comprising the steps of:

(S1) dissolving and dispersing the water-soluble component of the cosmetic at 30 to 80 캜;

(S2) dissolving the agar at 50 to 80 DEG C, and then mixing and dispersing the water-soluble component;

(S3) mixing the oil-soluble ingredient of the cosmetic composition into the dispersion obtained in the step (S2) and emulsifying and dispersing the mixture at 30 to 80 ° C at 1000 to 5000 rpm for 1 to 20 minutes; And

(S4) cooling and discharging the cosmetic composition obtained in the step (S3).

The agar used as an active ingredient in the cosmetic composition of the present invention is a form packed at a high temperature and utilizes the property of being gelled upon cooling, and generally used agar can be added at the time of manufacturing a cosmetic by heating at a temperature of 50 to 80 캜 .

At this time, agar may be added to the cosmetic composition in an amount of 0.001 to 50% by weight, preferably 0.1 to 30% by weight, more preferably 1 to 10% by weight based on the total weight of the composition. If the content of the agar is less than 0.001% by weight, the skin moisturizing effect is insufficient.

The water-soluble component used in the cosmetic composition of the present invention is generally a conventional water-soluble component used in a cosmetic composition, and its use is not particularly limited.

In addition, the oil-soluble ingredient used in the cosmetic composition of the present invention is generally an ordinary oil-soluble ingredient used in a cosmetic composition, and its use is not particularly limited.

Meanwhile, the present invention provides a cosmetic composition for moisturizing skin containing agar, which is produced according to the above-described method.

The moisturizing cosmetic composition according to the present invention can be used for enhancing skin barrier function and inducing differentiation of dermal keratinocyte. Therefore, it is possible to prevent or improve dry skin, atopic dermatitis, contact dermatitis or psoriasis caused by incompletion of epidermal differentiation And the like.

The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a cosmetic composition containing a lipid, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the cosmetic composition of the present invention may further contain a skin absorption promoting substance to increase skin moisturizing effect, skin barrier function enhancing effect, and skin keratinocyte differentiation inducing effect.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The present invention also provides a cosmetic method characterized by applying a cosmetic composition containing agar of the present invention to human skin.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin protection effect according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

When the cosmetic composition comprising the agar of the present invention is applied to human skin, it is possible to obtain a skin keratinocyte differentiation promoting effect, a skin barrier function restoration effect, and a skin moisturizing effect.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

≪ Test Example 1 > Effect of promoting keratinocyte differentiation of agar

Human keratinocytes were cultured in a culture flask and grown to about 80%. The agar was heated and dissolved at 60 DEG C as shown in the following Table 1, and then 1, 10, 20 and 50 And the mixture was treated for 4 days. Then, a denaturant such as urea, sodium dodecyl sulfate (SDS), and a reducing agent such as? -Mercaptoethanol were mixed to remove the dissolved protein. After that, the remaining horny layer was measured for absorbance at 310 nm to analyze the peptide concentration, and the amount of increase by each sample was compared with that of distilled water as a negative control (0%). The results are shown in Table 1 below.

Concentration (ppm) Promoting effect of keratinocyte differentiation (%) One 8 10 24 20 69 50 91 Negative control (distilled water) 0

As shown in Table 1, it was confirmed that the agar used in the present invention is excellent in promoting keratinocyte differentiation.

EXAMPLES 1 to 3 Production of softening water containing agar

The softening water containing agar was prepared according to the composition shown in Table 2 below.

Specifically, the water-soluble components in the components shown in the following Table 2 were put into a tank tank at 50 캜 to completely dissolve and disperse the water-soluble components.

On the other hand, the agar was completely dissolved at 60 DEG C and then added to the tank tank for dispersion. Next, an oil-soluble ingredient in the ingredients shown in the following Table 2 was added to the tank, emulsified and dispersed at 50 DEG C and 2000 rpm for 10 minutes, and then cooled to prepare an agar-containing softening water.

Component (% by weight) Example 1 Example 2 Example 3 Agar 1.0 3.0 5.0 1,3-butylene glycol 3.0 3.0 3.0 glycerin 5.0 5.0 5.0 PIZZY 1500 1.0 1.0 1.0 Allan Thomas 0.1 0.1 0.1 DL-Panthenol 0.3 0.3 0.3 Lee D. TEE-2NA 0.02 0.02 0.02 Benzophenone-9 0.04 0.04 0.04 Sodium hyaruronate 5.0 5.0 5.0 ethanol 10.0 10.0 10.0 Octidodeces-16 0.2 0.2 0.2 Polysorbate 20 0.2 0.2 0.2 Preservative, fragrance, pigment a very small amount a very small amount a very small amount Distilled water Balance Balance Balance Sum 100 100 100

Examples 4 to 6 Preparation of convergent lotion containing agar

The procedure of Example 1 was repeated to prepare a convergent lotion containing agar according to the composition shown in Table 3 below.

Component (% by weight) Example 4 Example 5 Example 6 Agar 1.0 3.0 5.0 1,3-butylene glycol 2.0 2.0 2.0 glycerin 2.0 2.0 2.0 Allan Thomas 0.2 0.2 0.2 DL-Panthenol 0.2 0.2 0.2 Lee D. TEE-2NA 0.02 0.02 0.02 Benzophenone-9 0.04 0.04 0.04 Sodium hyaruronate 3.0 3.0 3.0 ethanol 15.0 15.0 15.0 Polysorbate 20 0.3 0.3 0.3 Citric acid a very small amount a very small amount a very small amount Preservative, fragrance, pigment a very small amount a very small amount a very small amount Distilled water Balance Balance Balance Sum 100 100 100

Example 7 Production of Nutritional Lotion Containing Agar

The nutritional lotion containing agar was prepared according to the composition shown in Table 4 below, and was prepared in the same manner as in Example 1 above.

Component (% by weight) Example 7 Agar 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysolate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butyl glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Example 8 and Comparative Example 1 Preparation of nutritional cream containing agar

The preparation was carried out in the same manner as in Example 1, except that the nutritional lotion containing agar was prepared according to the composition shown in Table 5 below.

Component (% by weight) Example 8 Comparative Example 1 Agar 3.0 - Pro-type glycerin monostearate 2.0 2.0 Cetearyl alcohol 2.2 2.2 Stearic acid 1.5 1.5 Wax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan stearate 0.6 0.6 Hardened vegetable oil 1.0 1.0 Squalane 3.0 3.0 Mineral oil 5.0 5.0 Trioctanoin 5.0 5.0 Dimethicone 1.0 1.0 Sodium magnesium silicate 0.1 0.1 glycerin 5.0 5.0 Betaine 3.0 3.0 Triethanolamine 1.0 1.0 Sodium hyaruronate 4.0 4.0 Preservative, fragrance, pigment a very small amount a very small amount Distilled water Balance Balance Sum 100 100

<Test Example 2> Recovery effect of skin barrier function

In order to examine the skin barrier function recovery effect of the agar-containing cosmetic of the present invention, the following experiment was conducted. The experiment was repeatedly applied acetone to the skin of hairless mouse to deteriorate the barrier function. TEWL (transdermal water loss) was measured with an evaporimeter EP1 from servomed (Sweden) h, the nutritional cream containing agar in accordance with the present invention and the nutritional cream of Comparative Example 1 containing no agar were each applied to an area of 5 cm 2, , 2 hours, 4 hours, and 8 hours after the administration, and evaluated for the extent to which the barrier function was restored. At this time, a known lipid mixture (ceramide: 2: 1: 1 mixture of cholesterol: fatty acid) was used as a positive control. The results are shown in Table 6 below with reference to the time-dependent changes in skin moisture loss at the initial damaged barrier state (100%).

sample 0 hours 1 hours 2 hours 4 hours 8 hours Example 1 85 63 55 39 20 Comparative Example 1 85 80 77 75 73 Positive control group 88 66 60 50 29

As shown in the results of Table 6, it was confirmed that the skin barrier function recovery effect of Example 8, which is a cosmetic composition containing agar of the present invention, was superior to that of Comparative Example 1, and the known lipid mixture ceramide: cholesterol : fatty acid in a 2: 1: 1 mixture.

&Lt; Test Example 3 > Effect of improving skin moisturizing power

In order to examine the skin moisturizing effect of the agar-containing cosmetic of the present invention, the following experiment was conducted. Twenty-five to twenty-twenty patients without skin disease were divided into two groups of twenty persons per one group, and each group was compared with the nutritional cream containing agar according to the present invention and the agar- The nutritional cream of Example 1 was applied to the face and forehead twice a day for one month. The skin conductivity was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The skin conductivity was measured and the rate of increase was evaluated. The results are shown in Table 7 below.

sample One week after 2 weeks Four weeks Example 1 57 63 58 Comparative Example 1 11 13 11

As shown in the results of Table 7, the skin conductivity of Example 8, which is a cosmetic containing agar of the present invention, is much higher than that of Comparative Example 1. In general, the skin conductivity is proportional to the skin moisture content. From the above results, it can be seen that the cosmetic composition containing agar in the present invention keeps the skin moisture content higher than the other cosmetic ingredients. Therefore, the cosmetic composition containing agar of the present invention is excellent in the skin moisturizing power improving effect.

&Lt; Test Example 4 >

In order to examine the skin moisturizing effect of the agar-containing cosmetic of the present invention, the following experiment was conducted. Twenty twenty to forty persons without skin disease were divided into two groups of 20 persons per one group, and each group was compared with the nutritional cream containing agar according to the present invention and the agar containing no agar prepared in Example 8 The nutritional cream of Example 1 was applied to the face and forehead twice a day for one month. Evaluation of skin pulling, roughness, and elasticity changes after using Example 8 and Comparative Example 1 for the test subjects was carried out. The evaluation was divided into 1 to 5 points (satisfaction with the higher number) as the satisfaction of the product effect on each item of skin pull, roughness, and elasticity change. The results are shown in Table 8 below.

As shown in the results of Table 8, the case of Example 8, which is a cosmetic containing agar of the present invention, is superior to the case of Comparative Example 1 which does not contain agar, in that the effect of improving skin pulling, roughness and gloss is superior Could know.

According to the present invention, a cosmetic composition for skin moisturizing, which contains agar prepared by using the agar-like gelation property of agar as an active ingredient, is effective not only for skin moisturization but also for promoting differentiation of skin keratinocyte and restoring skin barrier function. The cosmetic composition according to the present invention contains agar as an active ingredient to promote differentiation of keratinocyte, restores skin barrier function, and relieves tension and roughness of the skin and increases elasticity. Thus, cosmetic composition for skin moisturizing Can be usefully used.

Claims (6)

A method for preparing a cosmetic composition for promoting skin moisturization and dermal keratinocyte differentiation comprising agar as an active ingredient, which comprises the steps of:
(S1) dissolving and dispersing the water-soluble component of the cosmetic at 30 to 80 캜;
(S2) dissolving the agar at 50 to 80 DEG C, and then mixing and dispersing the water-soluble component;
(S3) mixing the oil-soluble ingredient of the cosmetic composition into the dispersion obtained in the step (S2) and emulsifying and dispersing the mixture at 30 to 80 ° C at 1000 to 5000 rpm for 1 to 20 minutes; And
(S4) cooling and discharging the cosmetic composition obtained in the step (S3). delete The method according to claim 1,
Wherein the agar is added in an amount of 0.001 to 50% by weight based on the total weight of the composition, wherein the agar is used as an active ingredient. A cosmetic composition for promoting skin moisturization and dermal keratinocyte differentiation promotion comprising agar prepared by the production method of any one of claims 1 to 3 as an active ingredient. 5. The method of claim 4,
The cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the agar is used as an active ingredient to promote skin differentiation and dermal keratinocyte differentiation. delete

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