KR20050031211A - Cosmetic composition having anti-inflammatory, skin-protecting, skin-elastic effects which comprise mixed plants extract - Google Patents

Abstract

A cosmetic composition comprising mixed plant extracts is provided, which composition has anti-inflammatory, skin membrane-protecting, skin-elastic and skin cell-growing effects, so that the cosmetic composition shows improved skin elasticity enhancement, stratum comeum removement, and skin moisturing. The cosmetic composition comprises 0.01 to 10 %(w/v) of mixed plant extracts containing extracts of 35 to 45 wt.% of Angelica gigas Nakai, 35 to 45 wt.% of Cnidium officinale Makino, 15 to 25 wt.% of Rehmanniae Radix Preparata and 1 to 5 wt.% of Paeonia lactiflora PALL., wherein the mixed plant extracts are produced with vegetable squalane as an extraction solvent using supercritical extraction method; the supercritical extraction method employs carbon dioxide as carrier gas; and the composition has a formulation selected from skin lotion, nutritive lotion, nutritive cream, massage cream, nutritive essence, pack, make-up base, foundation and moisturing oil.

Description

Cosmetic composition having anti-inflammatory effect, skin protection effect and skin elasticity effect including mixed plant extracts {Cosmetic composition having anti-inflammatory, skin-protecting, skin-elastic effects which comprise mixed plants extract}

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition exhibiting an anti-inflammatory effect, skin protection effect and skin elasticity effect containing an extract obtained from the vegetable mixture of Angelica, Sukji, Cheongung, Peony.

Skin is the most important organ in our body that most closely contacts the outside environment. Skin can be divided into three categories: epidermis, dermis, and hypodermis. As the skin ages, typical symptoms of aging such as wrinkles, dry skin and sagging skin appear due to ultraviolet rays, pollution, and stress.

The skin is a complex organ composed of cells of various functions and corresponding physical properties. The dermal layer combines elastic solid materials (fibres) with viscous liquid materials to maintain inherent skin elasticity. . Thus, the skin responds to mechanical tension due to its unique properties, which is called viscoelasticity. Viscoelastic materials have a nonlinear functional relationship in the stress-strain function relationship, and viscoelastic properties in which the deformation gradually occurs over time in the time-strain function relationship. The skin has such viscoelasticity because collagen fibers and elastin fibers form a unique three-dimensional structure in the substrate of proteoglycans. Skin ages due to intrinsic and external factors (especially photoexposure). Photoaging that appears on the face, nape, back of the hand, etc., which are exposed to a lot of sunlight, results in thickening of the skin and elastic fibrosis in which deformed elastic fibers accumulate. On the other hand, natural aging that occurs in areas that are barely exposed to daylight, such as the abdomen or back, usually results in decreased skin function and atrophy, and decreases the number of skin cells or the thickness of the skin. As the aging progresses, the elastic fibers of the skin are deformed and the elasticity of the skin is reduced or the skin is stretched. Therefore, this research has made a lot of efforts to develop natural products that can help desquamation of the stratum corneum, hydration and elasticity of the stratum corneum. When it was not good, it was judged that the prescription of four kinds of medicines, Mt. Tang, Sukji Hwang, Cheongung, and Peony, would be useful for skin health.

Angelica is known to have a good effect on the blood circulation, and Angelica is recognized as a very useful substance for the skin containing vitamin B12, vitamin E, beta sitosterol (β-Sitosterol). Sukji sulfur contains Fe to help hematopoiesis and vitamin A to prevent skin keratinization and soften skin to soften keratinocytes. Cheongung contains ferulic acid, essential oils and caffeine to soothe the skin. Peony contains a lot of sugar, mucus, organic acids and trace minerals to help the skin's astringent action and moisturize the skin.

In addition, the present inventors have sought a method of extracting the components useful for the skin without destroying them, such as antioxidants possessed by four herbs such as Angelica, Sukji Hwang, Cheongung, Peony. As a result of the research, it is possible to develop a clear and clean natural oil using supercritical (ultra high pressure) extraction method using carbon dioxide and vegetable squalane without extracting using a solvent extraction method using methanol, ethanol and glycerin.

Therefore, the present inventors were able to develop various cosmetic products using natural oils obtained from the four natural products, and applied these cosmetics to the tester to compare the skin improvement state and the skin elasticity before and after use to confirm the very good experimental results. Thus, the present invention can be completed.

Accordingly, it is an object of the present invention to provide a mixed plant extract having an anti-inflammatory effect, skin protection effect and skin elasticity effect.

Another object of the present invention is to provide a cosmetic composition comprising an extract having such efficacy.

In view of this, the present invention is to search for a natural plant having an anti-inflammatory effect, skin protection effect and skin elasticity effect in search of useful natural products that make the skin elastic among various natural products, Angelica, Sukji Hwang, Cheongung It has been found that the plant mixture of peony is useful for inhibiting aging and improving elasticity. In the present invention, as a result of testing the anti-aging effect of the mixed plant extract obtained by extracting these four natural products in a constant mixing ratio, it was found that the anti-inflammatory effect, the protective effect of the cell membrane. In addition, it was found that even in a variety of cosmetic compositions, four natural substances were formulated at a certain concentration or more and applied directly to human skin, thereby exhibiting a distinct skin elasticity improvement effect, keratin improvement effect, and moisturizing effect.

In order to achieve the object of the present invention, the present invention has an anti-inflammatory effect, skin protection containing a mixed plant extract extracted from Angelica, Sukji Hwang, Cheongung, Peony which can prevent skin roughness, loss of elasticity and dry skin It provides a cosmetic composition having an effect and skin elasticity effect.

Preferably, the composition of the four medicinal ingredients in the present invention, the weight ratio of the Angelica 35-45%, 35-45% cheongung, 15-25% peony, crushed sulfur 1-5% by dry weight ratio The mixture is extracted so that the sum of the medicines is 100%.

Preferably, in the present invention, the mixed plant extract is characterized in that it is extracted using a vegetable squalane as the extraction solvent and using a supercritical extraction method. Phytosqualan is a highly concentrated nutritious oil extracted and purified from natural plants such as olives. Supercritical extraction method, unlike conventional heat extraction or solvent extraction, extracts only the necessary components of the plant at a high concentration at a high temperature at a low temperature to preserve the freshness of natural plants.

Preferably, in the present invention, the supercritical extraction method is characterized in that the extraction using carbon dioxide as a carrier gas. In the present invention, because it uses carbon dioxide harmless to the human body instead of the organic solvent, it is not toxic to the skin.

Preferably, the cosmetic composition of the present invention is characterized by containing the mixed plant extract in an amount of 0.01 to 10% (w / v) based on the whole composition. If the content is less than 0.01%, it is difficult to obtain the anti-inflammatory effect, skin protection effect and skin elasticity effect of the present invention, if it is higher than 10% it is difficult to maintain the properties of the desired cosmetic composition.

The mixed plant extract of the present invention is added to nutrition lotion (lotion), nutrition cream, essence, eye cream, pack, etc., lotion (skin lotion), nutrition lotion, nutrition cream, massage cream, nutrition essence, pack, makeup base, It provides a cosmetic composition having an anti-inflammatory effect, skin protection effect and skin elasticity, characterized in that the foundation, moisturizing oil.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Examples. These examples are only intended to illustrate the invention, and the scope of the invention is not to be construed as limited by these preparations and examples.

Preparation Example 1 of Plant Extract  :

Angelica 100g, Sukji sulfur 3.0g, Cheongung 100g, Peony 50g was ground and mixed with 150 g of vegetable squalane (phytosqualan) and stirred and left for 1 hour. The mixture was introduced into a supercritical extraction tank having an internal volume of 1.5 L, the extraction tank was sealed, and carbon dioxide was supplied to the extraction tank using a high pressure gas pump to increase the pressure. When the pressure of the extraction tank reached 300 atm, the supply of carbon dioxide was stopped, and the temperature inside the extraction tank was raised to 40 ° C. at a rate of 0.5 ° C./min using a heating wire wrapped around the outside using a PID temperature controller. The pressure of the extraction tank due to the elevated temperature was partially discharged using a pressure regulator attached to the outlet of the extraction tank to adjust the pressure to 300 atm, and the temperature and pressure of the extraction tank were maintained at 40 ° C. and 300 atm for 50 minutes. The high pressure gas pump was restarted and carbon dioxide was continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the extract was released using a pressure regulator attached to the outlet side, and the temperature and pressure of the extraction tank were kept constant. Due to the pressure and temperature drop released through the pressure regulator, carbon dioxide is transferred to the solid dry ice, which causes clogging of the tube on the final outlet side. Thus, the temperature of the pressure regulator was maintained at 70 ° C. The extracted extract was decompressed to atmospheric pressure to release carbon dioxide into the atmosphere, and the active ingredients of the four natural plants and phytoscualan were recovered together into the collector. The process was carried out for 8 hours to obtain a clear transparent extraction oil, weighed and finished the extraction operation. The final extract weighed 104g.

Preparation Example 2 of Plant Extract :

Angelica 80g, Sukji sulfur 3.0g, Cheongung 120g, Peony 50g was ground and mixed with 150g of vegetable squalane (phyto squalane) and stirred for 1 hour. And 110g was extracted by preparing a plant extract in the same manner as in Preparation Example 1 of the plant extract.

Preparation Example 3 of Plant Extract :

110g of Angelica, 3.0 g of Sookji sulfur, 100 g of Cheongung, and 40 g of Peony were ground and mixed with 150 g of vegetable squalane (phyto squalane), followed by stirring for 1 hour. And 115g was extracted by preparing a plant extract in the same manner as in Preparation Example 1 of the plant extract.

Preparation Example 4 of Plant Extract  :

Angelica 100g, Sukji sulfur 10g, Cheongung 110g, peony 33g was ground and mixed with 150g of vegetable squalane (phyto squalane) and stirred for 1 hour. In addition, 99 g was extracted by preparing a plant extract in the same manner as in Preparation Example 1 of the plant extract.

Experiment 1. Anti-inflammatory Experiment

The anti-inflammatory effect was measured for the preparation example of the extract mixture.

Experiment method

Before applying the sample containing the mixed plant extract with the left ear of the mouse as the control site and the right ear as the test site, the ears were washed thoroughly with ethanol, and 20 µl of the sample was continuously applied once a day for 4 days and after 1 hour of the last application. Ethanol was applied to the left ear and 2 mg / ear of arachidonic acid was applied to the right ear. After 1 hour, the degree of ear edema was measured three times by using a micrometer. The anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group and the results are shown in Table 1.

※ Inhibition Rate (%) = (A―B) / A × 100

A: average thickness of control ears (thickness of arachidonic acid-treated ears-thickness of untreated ears)

B: thickness of the ear of the mixed plant extract application group (thickness of sample-treated ear-thickness of untreated ear)

Anti-inflammatory Effects on Mixed Plant Extracts sample Was contained in a final concentration of half eungaek (㎍ / ml) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Indomethacin 1.0 ethanol 296 475 34.4 Arachidonic acid 2mg / ear ethanol 288 561 - Preparation Example 1 1.0 ethanol 320 522 26.0 Preparation Example 2 1.0 ethanol 297 513 20.8 Preparation Example 3 1.0 ethanol 266 499 14.6 Preparation Example 4 1.0 ethanol 310 509 27.1

Experiment 2. Cell Membrane Protection Experiment

The membrane protective effect of the extract mixture was measured.

Experiment method

Erythrocytes (leukocytes removed) used in the experiment were collected from Albino rabbits from New Zealand. The erythrocyte suspension had an OD of 0.6 at 700 nm and an erythrocyte count of 1.5 × 10 7 cells / ml. 50 μl of the mixed plant extract was added to the red blood cell suspension, pre-incubated in the dark for 30 minutes, and then light-sensitized (rose-bengal) was added. After 15 minutes of light irradiation, the degree of erythrocyte destruction by post-incubation was calculated from% transmittance at 700 nm at 15 minute intervals. The effect of the mixed plant extracts on photohemolysis was represented by τ50 indicating post-incubation time and degree of hemolysis. The τ50 value is the time taken for red blood cells to 50% hemolysis (minutes). The control is 1,3-butylene glycol.

Membrane Protective Effects on Mixed Plant Extracts Manufacture example Final concentration of the sample in the reaction solution (µg / ml) Cell membrane protective action ( τ ₅₀ : min)   Control 100 20  Preparation Example 1 100 223  Preparation Example 2 100 245  Preparation Example 3 100 220  Preparation Example 4 100 250

Experiment 3. Cell Proliferation Effect Experiment

The effect of cell proliferation was measured on the extract mixture.

Experiment method

100 μl of 5% fetal bovine serum (FBS) solution was added to a 96 microwell plate, and the sample was dispersed in a predetermined concentration in DMEM medium containing 2% FBS. Hemocytometer was used to disperse fibroblasts from neonatal foreskin tissue in 100 μl medium per well and incubate for 7 days. After incubation, the supernatant was removed and washed again by adding 200ul of 5% PBS (phosphate buffered saline), and 150 μl of DMSO was added per well, followed by shaking for 10 minutes to measure absorbance at 570 nm. At this time, the experiment was conducted in the same manner without adding the extraction mixture as a control group and the cell proliferation effect was compared from the difference in relative absorbance.

Cell Proliferation Effects on Mixed Plant Extracts Manufacture example Final concentration of the sample in the reaction solution (µg / ml)  Cell proliferation effect (%)  Preparation Example 1              10 104  Preparation Example 2              10 106  Preparation Example 3              10 103  Preparation Example 4              10 108

Example 1: nutrition lotion

Prescription examples of nutrient lotion in cosmetics containing mixed plant extracts are as follows.

number Raw material Example 1 Comparative Example 1 One   Mixed Plant Extract 3.0 - 2 Beeswax 1.00 1.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.5 0.5 5 Liquid paraffin 8.0 11.0 6 Squalane 3.0 3.0 7 Sorbitan stearate 1.0 1.0 8 Glyceryl Stearate / Pig-400 Stearate 1.2 1.2 9 Lipophilic monostearic acid glycerin 0.50 0.50 10 Stearic acid 1.50 1.50 11 glycerin 5.00 5.00 12 Butylene glycol 5.00 5.00 13 Carboxy Vinyl Polymer 0.1 0.1 14 Triethanolamine 0.2 0.2 15 antiseptic a very small amount a very small amount 16 Pigment a very small amount a very small amount 17 Spices a very small amount a very small amount 18 Purified water Remaining amount Remaining amount

<Manufacturing method>

Example 1: After mixing 11, 12, 13, 15, and 18 and heating to 70-75 ° C. and putting it into the manufacturing part, the emulsifier was activated and 1,2, 3, 4, 5, 6, 7, 8 , 9, 10, 14 is heated to 70 ~ 75 ℃ and added and emulsified. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, 15, 16, and 17 times are added thereto, re-emulsified, degassed, cooled to 25 ° C., and aged.

Comparative example 1: 11, 12, 13, 15, 18 while mixing and stirring, heated to 70 ~ 75 ℃ and put into the manufacturing unit, then the emulsifier is activated and 2, 3, 4, 5, 6, 7, 8, 9 , 10, 14 is heated to between 70 ~ 75 ℃ input and emulsified. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, 15, 16, and 17 times are added thereto, re-emulsified, degassed, cooled to 25 ° C., and aged.

Example 2 Nutrition Cream

Prescription examples of nutrient creams in cosmetics containing mixed plant extracts are as follows.

number Raw material Example 2 Comparative Example 2 One   Mixed plant extracts 3.0 - 2 Beeswax 1.8 1.8 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.5 0.5 5 Liquid paraffin 10.0 13.0 6 Squalane 5.0 5.0 7 Sorbitan stearate 1.00 1.0 8 Glyceryl Stearate / Pig-400 Stearate 1.00 1.0 9 Lipophilic monostearic acid glycerin 0.50 0.50 10 Stearic acid 1.50 1.50 11 glycerin 5.00 5.00 12 Butylene glycol 5.00 5.00 13 Triethanolamine 0.2 0.2 14 antiseptic a very small amount a very small amount 15 Pigment a very small amount a very small amount 16 Spices a very small amount a very small amount 17 Purified water Remaining amount Remaining amount

<Manufacturing method>

Example 2: After mixing 11, 12, 14, and 17 and heating to 70-75 ° C. and putting them into the manufacturing part, the emulsifier was activated and 1, 2, 3, 4, 5, 6, 7, 8, 9 , 10, 13 is heated to 70 ~ 75 ℃ to emulsify. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, and 14, 15, and 16 times are added thereto, re-emulsified, and then cooled to 25 ° C. and aged.

Comparative Example 2: 11, 12, 14, 17 while mixing and stirring to heat between 70 ~ 75 ℃ put into the manufacturing unit, and then actuated emulsifier 2, 3, 4, 5, 6, 7, 8, 9, 10 , 13 is heated to 70 ~ 75 ℃ to emulsify. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, and 14, 15, and 16 times are added thereto, re-emulsified, and then cooled to 25 ° C. and aged.

Example 3: Massage Cream

Among the cosmetics containing a mixed plant extract, a prescription example of a massage cream is as follows.

number Raw material Example 3 Comparative Example 3 One   Mixed plant extracts 3.0 - 2 Beeswax 2.0 2.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.8 0.8 5 Liquid paraffin 40.0 43.0 6 Squalane 5.0 5.0 7 Caprylic / Capric Triglycerides 4.0 4.0 8 Carboxy Vinyl Polymer 0.2 0.2 9 glycerin 5.0 5.0 10 Butylene glycol 3.0 3.0 11 Propylene glycol 3.0 3.0 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Remaining amount Remaining amount

<Production method>

Example 3 8, 9, 10, 11, 12, 13, 16 while mixing and stirring, heated to 70 ~ 75 ℃ to put into the manufacturing unit and then actuated emulsifier 1, 2, 3, 4, 5, 6, 7 is heated to 70 ~ 75 ℃ and emulsified. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, and 14,15 times are added thereto, re-emulsified, and then cooled to 25 ° C. and aged.

Comparative Example 3: 8, 9, 10, 11, 12, 13, 16 while mixing and stirring to heat between 70 ~ 75 ℃ put into the manufacturing unit, and then actuated emulsifier and 2, 3, 4, 5, 6, 7 It is emulsified after heating to 70 ~ 75 ℃. After emulsification, the mixture is cooled to 50 ° C. while stirring using a stirrer, and 14,15 times are added thereto, re-emulsified, and then cooled to 25 ° C. and aged.

Example 4: Nutritional Essence

Among the cosmetics containing a mixed plant extract, a prescription example of a nutritional essence is as follows.

number Raw material Example 4 Comparative Example 4 One   Mixed plant extracts 3.0 - 2 Squalane 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.0 1.0 6 cholesterol 0.2 0.2 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 12.0 15.0 10 Carboxy Vinyl Polymer 0.2 0.2 11 Xanthan Gum 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Example 4 Raw materials 1, 2, 3, 4, 5 and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 1, 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 9 is gradually added and homogenized at a constant temperature, and then again the microflu Pass it through the die again. 10, 11, 12, 13 is added to disperse to stabilize and mature.

Comparative Example 4: The raw materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and the raw materials 7, 8 and 14 are mixed and homogenized at a constant temperature and passed through a microfluidizer, and then the raw material 9 is gradually added and homogenized at a constant temperature, and then again added to the microfluidizer. Pass it again. 10, 11, 12, 13 is added to disperse to stabilize and mature.

Experimental Example 1. Experiment to confirm the effect of improving skin condition

When applying the skin to the nutrition cream of Example 2 and Comparative Example 2, the following clinical confirmation experiment was carried out to confirm the skin condition improvement effect. Fifty-five owners of normal, oily, dry, and combination skins were composed of 25% of women aged 20 to 55 years. To the same person, the nutritional cream of Example 2 was applied to the upper part of the face, and the nutritional cream of Comparative Example 2 was applied to the skin once a day for 20 days once every day after washing the face, and then the skin condition was improved to confirm the improvement of the skin condition. The degree was measured. The test results are shown in Table 8.

Confirmation of skin condition improvement effect                  Survey Items Skin condition improvement Remarks Number of people % Example 3 very good 25 50.0 good 10 20.0 is average 15 30.0 so so - - Comparative Example 3 very good - - good 9 18.0 is average 15 30.0 so so 26 52.0

It can be seen that the skin improvement effect is superior to Comparative Example 2 prepared without adding the cosmetic of Example 2 prepared by adding the mixed plant extract as shown in the survey results.

Experimental Example 2. Skin elasticity increase test (1)

In order to measure the effect of increasing the elasticity of the skin when the nutrient lotion of Example 1 and Comparative Example 1 was applied to the skin, 20 to 55-year-old females face the owners of normal, oily, dry and combination skin. On the left part, the nutrition lotion of Example 1 was applied, and on the right part of the face, the nutrition lotion of Comparative Example 1 was applied twice a day in the morning and evening, and then the skin elasticity after 1, 2, 3 and 4 weeks. The elasticity of the skin was measured using a ballistometer. The measurement results are shown in the following Table 9 and FIG.

Skin elasticity increase test (1) result            Sample Survey Items Cosmetics of Example 1 Cosmetics of Comparative Example 1 ratio Go T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄ Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 amplitude first 1.6 5.3 6.5 6.9 7.5 1.6 2.9 3.5 3.8 4.0 second 1.5 3.6 5.3 6.3 7.1 1.6 2.1 2.6 2.9 3.0 third 0.0 3.9 4.8 5.4 6.0 0.0 0.0 2.2 2.4 2.6 fourth 0.0 0.0 2.1 4.9 5.3 0.0 0.0 0.0 0.0 0.0 Average 0.77 3.2 4.675 5.875 6.475 0.80 1.25 2.075 2.275 2.40

(Note) T0; Before use T1; T2 after 1 week of use; After 2 weeks of use T3; After 3 weeks of use T4; After 4 weeks

As can be seen from the above results, the skin elasticity is improved by the skin condition improvement effect of the plant mixture extract compared to the nutrient lotion of Comparative Example 1 prepared without the nutrition lotion of Example 1 containing the plant mixture extract according to the present invention. It can be seen that there is an effect.

Experimental Example 3. Skin elasticity increase test (2)

Example 4 was applied twice a day to the left eye edge of 20 people to test the effect of the plant mixture extract on skin elasticity. Comparative example 4 was applied to the right eye edge. After 14 days of treatment, skin elasticity of the skin surface was measured by Cutometer SEM 474. Skin elasticity measurement by Cutometer SEM 474 is a measure of the depth of wrinkles, the smaller the value is the better elasticity. The elasticity value was shown as a percentage decrease compared to the control, and the average value of 20 subjects was shown in the results. The control is the measurement before processing the sample.

Skin elasticity increase test result sample Skin elasticity (wrinkle depth reduction) effect (%) 15th 30 days Example 4 8.0 17.3 Comparative Example 4 2.1 5.8

As described above, according to the present invention, due to the excellent anti-inflammatory effect, cell membrane protection effect, and cell proliferation effect of the mixed plant extract obtained from Angelica, Sukji Hwang, Cheonggung, Peony, the skin elasticity improvement effect in various cosmetic compositions containing the same , Exfoliation effect, moisturizing effect can be exhibited.

1 is a graph showing the anti-inflammatory effect on the mixed plant extract,

Figure 2 is a graph showing the cell membrane protective effect on the mixed plant extract,

3 is a graph showing the cell proliferation effect on the mixed plant extract,

Figure 4 is a graph showing the skin elasticity test results of Example 1 of the present invention.

Claims (6)

Cosmetic composition having an anti-inflammatory effect, skin protection effect and skin elasticity, containing a mixed plant extract extracted from Angelica, Sukji Hwang, Cheongung, Peony. The method of claim 1, wherein the weight ratio of the dried Angelica 35-45%, Cheongung 35-45%, Peony 15-25%, Sukji sulfur is characterized by containing a mixed plant extract extracted by mixing 1-5% Cosmetic composition having anti-inflammatory effect, skin protection effect and skin elasticity effect. According to claim 1, wherein the mixed plant extract is a cosmetic composition having an anti-inflammatory effect, skin protection effect and skin elasticity, characterized in that extracted using a vegetable squalane as the extraction solvent and using a supercritical extraction method. The cosmetic composition having an anti-inflammatory effect, skin protection effect and skin elasticity effect according to claim 3, wherein the supercritical extraction method uses carbon dioxide as a carrier gas. The cosmetic composition having an anti-inflammatory effect, skin protective effect and skin elasticity effect according to claim 1, wherein the mixed plant extract is contained in an amount of 0.01 to 10% (w / v) based on the whole composition. The anti-inflammatory composition according to any one of claims 1 to 4, wherein the cosmetic composition is a lotion (skin lotion), nutrition lotion, nutrition cream, massage cream, nutrition essence, pack, makeup base, foundation, moisturizing oil. Cosmetic composition having the effect, skin protection effect and skin elasticity effect.