KR20100057157A - Cosmetic composition containing the extract of prunella vulgaris and artemisia abrotanum for improvement of wrinkle - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Prunella vulgaris and Artemisia abrotanum extract for anti-wrinkle is provided to promote elastine biosynthesis and triglyceride synthesis and to suppress elastase activation. CONSTITUTION: A cosmetic composition for anti-wrinkle contains 0.005-20 weight% of Prunella vulgaris and Artemisia abrotanum extract. The Prunella vulgaris extract promotes elastine synthesis and suppresses elastase activation. The Artemisia abrotanum extract promotes adipocyte differentiation and triglyceride synthesis. The composition is used in the form of skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, massage cream, hand cream, essence, pack, shampoo, cleansing foam, cleansing lotion, body lotion, loose powder, or eye-shadow.

Description

Cosmetic composition for improving wrinkles containing Prunella vulgaris and Prunus locust extract {Cosmetic Composition containing the Extract of prunella vulgaris and Artemisia abrotanum for Improvement of Wrinkle}

The present invention relates to a cosmetic composition for improving wrinkles containing Prunella vulgaris extract and Artemisia abrotanum extract, and in particular, contains a Hachocho extract, which promotes elastin biosynthesis and inhibits elastase activity. Containing the extract of R. japonica, it relates to a cosmetic composition for improving wrinkles having an excellent effect of promoting differentiation of adipocytes and synthesis of triglycerides and activating G3PDH (Glyceraldehyde-3-phosphate dehydrogenase), which is a major enzyme of fat accumulation. . In addition, Hachocho extract and P. locust extract have excellent antioxidant effects.

Cosmetics are deeply involved in our daily lives and used by many people. Before the development of human civilization, the purpose of cremation was to protect the body from nature.However, with the development of science and civilization, the purpose of cremation is to keep the body clean and beautiful, and to delay the aging to make a beautiful and pleasant life. Changed. The function of cosmetics is not only clean and aesthetic, but also free radicals and free radicals caused by external factors such as moisture, pollutants and stress, ultraviolet rays, and internal factors such as deterioration of immune cells and deterioration of cell activity. This is to remove or suppress the cause of skin aging such as protein, nucleic acid, and cell membrane lipid destruction by peroxides. In addition, cosmetics having whitening, skin wrinkle improvement and suppression, and sunscreen functions to meet the consumer's desire for high-performance cosmetics continue to be developed. In particular, wrinkle improvement and suppression products are of great interest to consumers seeking to stay younger.

Wrinkles are formed by repetitive movements of muscles in a specific direction for a long time, affected by age, external environment, and UV irradiation. Damage to skin cells caused by these factors causes various aging phenomena. The collagen, elastin, and GAGs constituting the dermal matrix are not easily produced and degraded, resulting in poor firmness and wrinkles of the skin.

In addition, the subcutaneous fat layer that supports the skin under the dermis decreases the production of fat as it ages and becomes thinner, thereby making the skin thinner, less elastic, and affecting wrinkle formation. To maintain the subcutaneous fat layer, which plays a large role as a supporting layer, it is necessary to activate fat cells and promote fat production.

Currently, wrinkle improvement cosmetics are known as retinoids, adenosine, animal placental protein, chlorella extract and the like. The most well known retinol is a substance that promotes collagen synthesis, but it is unstable and has limited use due to safety problems such as irritation and seizures when applied to the skin. It is known to be difficult.

As a result of the study of various herbal extracts to solve the above problems, the object of the present invention is to contain haegocho extract to promote elastin biosynthesis, to inhibit the elastase activity, and to differentiate the adipocytes by containing the extract And to provide a cosmetic composition that not only exhibits the effect of promoting the synthesis of triglycerides and activating G3PDH, a major enzyme of fat accumulation, but also shows an antioxidant effect.

In order to solve the above-mentioned problem, the cosmetic composition containing the hachocho extract and Gaechuldae extract according to the present invention may include the following means.

The cosmetic composition for improving wrinkles according to an embodiment of the present invention contains a hyacinth extract and an extract of Sageumja.

Said Hagocho extract is characterized by promoting elastin synthesis and inhibiting elastase activity.

In addition, the extracts of R. oleracea promote the differentiation of adipocytes and the synthesis of triglycerides and are characterized by the activation of G3PDH, a major enzyme of fat accumulation.

On the other hand, the cosmetic composition is characterized by having an antioxidant effect.

On the other hand, the content of the haegocho extract and Gaeoksae extract is characterized in that 0.005 to 20% by weight relative to the total weight of the cosmetic composition.

The composition includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam , Cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder and eye shadow, characterized in that it is formed of any one formulation selected from the group consisting of.

The cosmetic composition containing the extract of Hagocho extract and Gaejanggyeom extract according to the present invention is effective in improving skin wrinkles, and in particular, it contains the Hagocho extract, which promotes elastin biosynthesis and inhibits elastase activity. It promotes the differentiation of adipocytes and the synthesis of triglycerides, and activates G3PDH, a major enzyme of fat accumulation. In addition, Hachocho extract and Prunus locust extract also have antioxidant effects. The cosmetic composition containing the Hachocho extract and Prunus locust extract is useful for the cosmetic composition for preventing skin aging through the wrinkle improvement effect.

Hereinafter, the present invention will be described in detail.

Hagocho is also called nectar and is a perennial herb with nectar. It is native to Korea and Manchuria. It is a perennial herb and grows in mountain fields and roadside grass fields with a height of 20-30cm. The main stem is square, with white hairs all over, and the flowers are purple and close to the flowers. The leaves face each other and have a serrated tooth shape. Use dried herbs or flowers of nectar as a Chinese medicine. Ingredients include triterpenoids, oleanolic acid, ursolic acid, rutin, hyperoside, and caffeic acid. In civilian use, it is used for tonic, hypertension, uterus, menstrual irregularity, diuresis, eye disease, goiter, gonorrhea, nausea, head, fever, playing window, fever and fever. It is used for various treatments such as adenocarcinoma and lymph node tumors. It also has the effect of inhibiting the growth of pathogenic microorganisms such as tuberculosis bacilli, dysentery bacilli, staphylococci, and hemolytic streptococci. According to <Agreement Relief>, it is effective for urinary stones and removes the heat of the liver and phlegm and improves eye diseases, headaches and dizziness. In <Dictionary Synonym>, there are detoxification, coercive action, diuretic action, bacteriophage, etc. It is said that it can be used for playing window, permanent, yeastitis, head window, carcinoma, hepatic, edema, lobster, hypertension, pulmonary tuberculosis, epidemic lobe. Chinese medicinal dictionaries published in China have been shown to be effective in Nag, permanent, acute mastitis, breast cancer, eye pain, and dizziness. Jeonnam Herbal Herbs is said to be effective in the treatment of wounds cut in guan wasa, bruises and knives. Korea Herbal Herbs Book is said to be effective in tuberculous lymphadenitis, boils, and hypertension.

Gaesae Chrysanthemum is a biennial plant of the dicotyledon plant Lantern Asteraceae. It grows in the sandy grounds of streams. It is 40-150cm in height. Hairless and smelly throughout. The leaves attached to the roots are clustered and finely divided, and the pieces are string-shaped, uneven and disappear when the flowers bloom. The upper leaves split again and the pieces are threaded. The leaves on the stem are shifted, long oval, and deeply divided into feathers. It is 9-15cm long, 3.5-5.5cm wide, with a deep toothed tooth, and the leaf size decreases as it goes up. Flowers bloom greenish yellow in July-September, 3.5-4 cm long, 5-6 mm in diameter, hanging on the ends of branches and stems, with flower beds 2-6 mm long. The gun pieces are rounded and arranged in three rows. The outer piece is slightly shorter in length and the middle and inner pieces are the same length. Female flower is about 1.5mm long corolla, and about 1.8mm bisexual. Fruits are achene, hairless, about 1mm long. Young leaves are edible. In oriental medicine, it is used as a medicine for colds, tuberculitis, malaria, hepatitis, cholecystitis, cholecystitis and jaundice. It is distributed in Korea (Jeju, Gangwon, Gyeonggi, Yellow Sea), Japan, and China.

Hagocho extract and Gaechuldae extract according to the present invention can be prepared as follows, respectively or simultaneously.

The vinegar and / or evergreen mugwort are washed thoroughly with water and then dried in the shade. Dried hachoweed and P. locust are put in the extraction container, and extracted for a predetermined time at an appropriate temperature in an appropriate amount of the extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. Preferably, hot water is extracted by adding ethanol.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of water bath extraction.

The deposition temperature is 30 ~ 70 ℃, more preferably 40 ~ 60 ℃, most preferably maintained at about 50 ℃ is most effective in the prevention of decay, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After obtaining the haegocho extract and / or japonica extract, solids are removed using a filter paper, the suspension is centrifuged, and the supernatant may be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

Hachocho extract used in the present invention is excellent in anti-wrinkle effect, promotes elastin production, inhibits elastay activity, indicates the use of skin condition improvement, and improves wrinkles of skin by the effect of promoting cell proliferation. Excellent stability in the formulation. In addition, it contains extracts of S. aureus and promotes the differentiation of adipocytes and the synthesis of triglycerides, and activates G3PDH, a major enzyme of fat accumulation. This fact is specified in the following examples.

In the composition of the present invention, the edible hydrangea extract and Gaechuldae extract may be contained 0.005 to 20% by weight, respectively, based on the total weight of the cosmetic composition.

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the haegocho extract and Gaechuldae extract.

Cosmetics prepared with a cosmetic composition containing the extract of Hagocho extract and Gaejanggyeok of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition, cosmetics containing the extract of Hagocho extract and Prunus locust extract of the present invention can be prepared in the form of adjuvants that can be applied topically or systemically, which are commonly used in the field of dermatology by containing a dermatologically acceptable medium or base. have.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the Hagocho extract and the extracts of Gacha myrtle extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances , Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological arts, such as any other ingredients commonly used in the art. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, etc. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited thereto.

Preparation Example 1 Preparation of Seaweed Extract

50 g of vinegar was finely crushed in a crusher, immersed in 200 ml of 100% ethanol, and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to 0.01 to 20% by weight to prepare an extract.

Preparation Example 2 Preparation of Echinacea Extract

50 g of ginseng japonica were finely crushed in a crusher, immersed in 200 ml of 100% ethanol, and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to 0.01 to 20% by weight to prepare an extract.

Experimental Example 1 Cell Proliferation Effect

In order to see the skin cell proliferation effect of Prunella vulgaris and Prunus locust extract, the human fibroblasts were added to 1 × 10 ⁴ cells in each well of a 96-well microplate. Inoculate and incubate for 24 hours in DMEM (Dulbecco's Modified Eagle's Medium) medium. After the culture, the extracts of Preparation Examples 1 and 2 prepared above were replaced with DM medium without serum adjusted to a final concentration of 50 to 500 µg / ml, followed by further incubation for 24 hours. MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium, 3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium after incubation : 5 mg / ml) 10 µl of solution was added thereto, and the reaction solution was removed after standing for 4 hours. 100 μl of dimethyl sulfoxide solution was added to each well to dissolve the stained cells, and the absorbance at 570 nm was measured with a microplate reader.

By performing the above procedure, the cell proliferation effect was obtained from Equation 1 below, and the results are shown in Table 1 below.

Cell proliferation effect (%) = {(absorbance at extract treatment-absorbance of control) / absorbance of control} X 100

Cell proliferation effect (%) Sample (µg / mL) Hagocho extract Mugwort Extract Control - - 50 38.2 0.2 100 51.9 1.3 200 70.5 5.7 300 75.8 10.4 500 83.7 15.6

According to the above Table 1, Hachocho extract can be confirmed that the cell proliferation effect is excellent in improving the skin wrinkles.

Experimental Example 2 Elastin Synthesis Effect

Human normal fibroblasts isolated from 10 ⁵ newborn skins were cultured in 6-well plates for 24 hours, and then the extract samples of Preparation Examples 1 and 2 were treated with 50 to 500 µg / ml. After 24 to 48 hours of incubation, the amount of elastin newly released by the fibroblasts was measured using a commercially available Fastin ™ elastin assay kit. The amount of elastin was corrected by cell number or protein amount. The results are shown in Table 2.

Elastin Synthesis Increase (%) Sample (µg / mL) Hagocho extract Mugwort Extract Control - - 50 18.9 0.4 100 34.2 0.7 200 65.2 2.4 300 79.8 5.9 500 85.4 10.4

According to the above Table 2, Hachocho extract shows an excellent effect on the increase in elastin synthesis, it can be confirmed that there is a skin wrinkle improvement effect through this.

Experimental Example 3 Elastase Inhibition Effect

When elastins, an enzyme that degrades elastin, is active, elastin, an elastic fiber, is broken down to accelerate skin wrinkle formation. Therefore, in this experimental example, the inhibition rate of the elastase of the extracts of Preparation Examples 1 and 2 was measured. Degradation of N-succinyl- (alanine) -3-p-nitroanilide, which is the substrate of elastase, results in p-nitroaniline, which is yellow in color. The degree of color development was measured by absorbance using a spectrophotometer to evaluate the rate of inhibition of elastase activity (The synthesis and analytical use of a high sensitive and convenient substrate of elastase, Biochemical Medicine 11, 350-357, 1974). The method of calculating the activity inhibition rate of the elastase is shown in Equation 2 below, and the detailed method is as described in the above document. The results are shown in Table 3.

% Elastase activity inhibition = (1-B / A) × 100

A: OD 400 without inhibitor

B: OD 400 in the presence of inhibitor

Elastase Synthetic Inhibitory Effect (%) Sample (µg / mL) Hagocho extract Mugwort Extract Control - - 50 10.3 1.4 100 22.6 2.6 200 40.2 4.1 300 62.5 13.5 500 70.5 21.6

According to the Table 3, the Hachocho extract is excellent in inhibiting the elastase synthesis can be confirmed that there is an effect to improve the skin wrinkles.

Experimental Example 4 Antioxidant Effect

In order to confirm the antioxidant efficacy, a free radical 1,1-diphenyl-2-picrylhydrazyl (1,1-Diphenyl-2-picrylhydrazyl; hereinafter referred to as DPPH) was used. DPPH was used by dissolving in ethanol to 2 × 10-4 M concentration. 0.15 mL of DPPH, 0.15 mL of the extract samples (1000 ppm-30 ppm) or vitamin C (1000 ppm-30 ppm) of Preparation Examples 1 and 2 were added thereto, and reacted at room temperature for 30 minutes, and then the absorbance was measured at 570 nm. . For DPPH scavenging effect, IC ₅₀ (50% Inhibitory activity) was compared with respect to a sample having a DPPH radical scavenging activity value of 50% or more calculated using Equation 3 below.

DPPH radical scavenging activity (%) = ((A control-B control)-(A sample-B sample)) / (A control -B control) × 100

A control: Absorbance of control group without sample

A sample: Absorbance of reaction group to which sample is added

B control: Absorbance of control group without DPPH

B sample: Absorbance of reaction group without DPPH

As a result, Table 4 shows that the scavenging effect of the free radicals using the DPPH of Hachocho extract, Gaecho wormwood extract, vitamin C (Roche, Switzerland).

Sample (µg / mL) SC ₅₀ Vitamin C 84.7ppm Hagocho extract 0.89% Mugwort Extract 1.32%

According to the Table 4, it was confirmed that the extract of Hachocho and Gaechuldae had an antioxidant effect.

Experimental Example 5 Fat Cell Differentiation Promotion Effect

In order to confirm the effect of adipocyte differentiation between the extracts of Prunella vulgaris and the extract of S. aureus, the S. aureus and S. aureus extracts were treated for 6 days at concentrations of 0.125, 0.25 and 0.5% in adipocytes (3T3 F442A cells). After removing the medium, washed with PBS, and stained the intracellular fat component using Oil red O reagent to selectively stain fatty acids.

Fat cell differentiation promoting effect (%) sample(%) Hagocho extract Mugwort Extract 0.125 0.2 15 0.25 0.3 45 0.5 1.2 96

As shown in Table 5, the control group that did not process the sample did not produce differentiation of adipocytes, resulting in a minimal fat content of about 5%. I could see what happened.

Experimental Example 6 PPAR-r mRNA Expression Promoting Effect

The purpose of this study was to investigate the effects of P. aeruginosa and P. aeruginosa extract on the mRNA expression of PPAR-r (Peroxisome proliferator activated receptor-r), which plays an important role in the formation and storage of adipose tissue. Adipocytes (3T3 F442A cells) were treated for 6 days at concentrations of 0.125, 0.25 and 0.5% in adipocytes in differentiation stages. After removing the medium, the cells were washed with PBS, the cells were recovered with trypsin-EDTA, and intracellular mRNA was extracted. PPAR-r mRNA expression was quantified by quantitative PCR.

PPAR-r mRNA expression promoting effect (%) sample(%) Hagocho extract Mugwort Extract 0.125 - 33 0.25 - 54 0.5 - 76

As shown in Table 6, it was confirmed that the amount of PPAR-r mRNA expression increased in proportion to the concentration of M. aureus.

Experimental Example 7 Triglyceride Formation Promoting Effect

In order to confirm the effect of promoting the production of fat (triglyceride) of the extracts of Prunella vulgaris and P. aeruginosa extracts, the extracts of P. aeruginosa were treated for 6 days at concentrations of 0.125, 0.25 and 0.5% in adipocytes (3T3 F442A cells). After removing the medium, washed with PBS, cells were recovered using trypsin-EDTA. The cells were crushed to extract intracellular triglycerides and quantified using a triglyceride measurement kit.

Triglyceride production promoting effect (%) sample(%) Hagocho extract Mugwort Extract 0.125 0.1 24 0.25 0.5 50 0.5 1.6 68

As shown in Table 7, it was found that the triglyceride production amount increased when the extracts were applied.

Experimental Example 8 G3PDH mRNA expression promoting effect

The purpose of this study was to investigate the effects of Prunella vulgaris and Prunus barberry extract on the mRNA expression promotion of G3PDH, which plays an important role in triglyceride storage. Adipocytes (3T3 F442A cells) were treated for 6 days at concentrations of 0.125, 0.25 and 0.5% in adipocytes in differentiation stages. After removing the medium, the cells were washed with PBS, the cells were recovered with trypsin-EDTA, and intracellular mRNA was extracted. G3PDH mRNA expression was quantified by quantitative PCR.

G3PDH mRNA expression promoting effect (%) sample(%) Hagocho extract Mugwort Extract 0.125 - 9 0.25 - 15 0.5 - 25

As shown in Table 8 above, it was confirmed that the amount of G3PDH mRNA expression increased in proportion to the concentration of the wormwood.

Preparation Example 3 Examples and Comparative Examples

To prepare a cosmetic composition containing the extract of Hagocho extract of Preparation Example 1 and the extract of Gacha myo japonicum of Preparation Example 2, to prepare a cosmetic composition lacking the haegocho extract and Gaja wormwood extract was prepared in the composition ratio of Table 9.

division Content (% by weight) ingredient Example 1 Example 2 Example 3 Comparative example Hagocho extract 0.5 1.0 - - Mugwort Extract 0.5 - 1.0 - Butylene glycol 2.0 2.0 2.0 2.0 Propylene glycol 6.0 6.0 6.0 6.0 Carboxy Vinyl Polymer 0.5 0.5 0.5 0.5 Beeswax 2.0 2.0 2.0 2.0 vaseline 7.0 7.0 7.0 7.0 Polysorbate 60 2.0 2.0 2.0 2.0 Sorbitan sesquioleate 2.5 2.5 2.5 2.5 Squalane 3.0 3.0 3.0 3.0 Liquid paraffin 10.0 10.0 10.0 10.0 Triethanolamine 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 Spices, preservatives a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Sum 100 100 100 100

Experimental Example 5 Clinical Effect of External Wrinkle Improvement

The clinical effect of skin wrinkle improvement on cosmetics containing Hachocho extract and Prunus locust extract of the present invention was measured. Forty women aged 40 or older (mean age 46.7 years) were divided into four groups (A, B, C, and D): Example 1 for Group A, Example 2 for Group B, and Example 3 for Group C After applying 12 weeks (2 times / day, 0.2g / time) around the wrinkled eyes, the comparative example was applied to group D, and the skin vismeter (Skin Visiometer SV 600 FireWire, CK Electronic GmbH) ) And the elasticity measuring instrument (Cutometer MPA 580, CK Electronic GmbH) were used to measure the wrinkle improvement and elasticity of the skin, and the results are shown in Table 10.

division Wrinkle improvement effect Elasticity Average depth of wrinkles (%) Roughness average (%) Skin adsorption degree (%) Example 1 T0 100.0 100.0 100.0 T12 69.4 60.4 56.1 Example 2 T0 100.0 100.0 100.0 T12 75.5 62.4 66.5 Example 3 T0 100.0 100.0 100.0 T12 82.9 63.7 59.4 Comparative example T0 100.0 100.0 100.0 T12 99.1 96.4 97.5

In Table 10, the average depth of the wrinkles is divided into five equal parts of the sample made of silicone rubber at the site where wrinkles are to be measured, and the average value of the difference between the highest and lowest points of the wrinkle height in each of five places. The roughness mean represents the mean value of the difference in wrinkle depth at each measurement site (dividing the whole into 255 measurement site points) from the average value of the total wrinkle depth in the sample. The lower the average depth and roughness of the wrinkles, the smaller the wrinkles are, and the smaller the skin aging is.

From the results shown in Table 10, when looking through the relative numerical calculation before / after the application of Examples 1, 2, 3, the average depth and roughness average of the wrinkles is carried out when the average depth value of the wrinkles when viewed as 100% Example 1 showed the best improvement, and Examples 2 and 3 were next. Therefore, it can be seen that the Hagocho extract and Gaechuldae mugwort extract-containing cosmetics according to the present invention have a considerable effect on the improvement of wrinkles of the skin.

In addition, in Table 10, the degree of adsorption of skin indicates the degree of adsorption of the skin into the cutometer measuring device (penetration depth of the skin into the aperture). When looking at 100% before application it can be confirmed that the degree of adsorption after the application of Examples 1, 2 and 3 is reduced by a significant degree of skin elasticity.

As a result, the fact that can be confirmed through the results shown in Table 10, the cosmetics containing the extract of Hagocho extract and Gaejanggyeokji extract according to the present invention to improve the skin wrinkle effect and elasticity effect, the excellent effect of preventing skin aging It can be seen that it is.

On the basis of the results of the previous experimental example, to present a cosmetic composition containing the extract of Haechocho and Gaejanggyeom of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Flexible Lotion

Examples of the formulation of the flexible lotion containing the Hagocho extract and Prunus locust extract were prepared as shown in Table 11 below.

Raw material Content (% by weight) Hagocho extract 1.00 Mugwort Extract 1.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Balance

Formulation Example 2 Nutrition Cream

Formulation example of the nourishing cream containing the Hagocho extract and Prunus locust extract was prepared as shown in Table 12.

Raw material Content (% by weight) Hagocho extract 2.0 Mugwort Extract 2.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Balance

Claims (6)

A cosmetic composition for improving wrinkles, comprising a Hagocho extract and Prunus locust extract. The cosmetic composition for improving wrinkles according to claim 1, wherein the edible extract is characterized by promoting elastin synthesis and inhibiting elastase activity. The cosmetic composition for improving wrinkles according to claim 1, wherein the extract of R. oleracea promotes the differentiation of adipocytes and the synthesis of triglycerides and the activation of G3PDH, which is a major enzyme of fat accumulation. The cosmetic composition for improving wrinkles according to claim 1, wherein the cosmetic composition has an antioxidant effect. The cosmetic composition according to any one of claims 1 to 4, wherein the content of the edible vinegar extract and the locust extract is 0.005-20 wt%, respectively, based on the total weight of the cosmetic composition. The composition of any one of claims 1 to 4, wherein the composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream , Essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow Cosmetic composition to be.