KR20080071386A - Anti-wrinkle cosmetic compositions containing extracts of spica prunellae - Google Patents

Abstract

Cosmetic compositions containing the extract of Prunella vulgaris L. are provided to inhibit activity of elastase, prevent reduction of collagen and elastin, inhibit or remove free radical production and inhibit side effects on the skin by using natural material, so that the compositions are useful for improving skin wrinkles. A cosmetic composition for improving skin wrinkles contains 0.0001-10.0 wt.% of the extract of Prunella vulgaris L. which is prepared by heating Prunella vulgaris L. in at least one solvent selected from water, C1-C4 anhydrous or hydrous alcohol, ethylacetate and acetone, concentrating the extract of Prunella vulgaris L. under reduced pressure, and freeze-drying or spray-drying the concentrated extract of Prunella vulgaris L.

Description

Anti-wrinkle cosmetic compositions containing extracts of Spica Prunellae

Skin is an important organ that is responsible for various physiological functions, such as barrier function, temperature control, and excretion, which protect the human body from the external environment and prevent internal moisture and useful ingredients from leaking out.

However, as the skin ages, the epidermis, dermis and subcutaneous tissue become thinner, which lowers the barrier function of the skin and the skin's physiological function decreases due to a drastic decrease in the moisture content of the skin. In addition to the aging phenomenon according to the age may be a skin aging due to light as a representative cause of aging. Wrinkle formation by skin aging by light is as follows.

First, fibroblasts present in the dermal layer of the skin are important cells that produce collagen. Collagen plays an important role in skin constituents such as mechanical firmness of skin, resistance of connective tissue, adhesion of tissue and support of cell adhesion. The collagen is deteriorated in the function of fibroblasts by photoaging due to aging and UV irradiation, and the production amount thereof is reduced, and collagen reduction is promoted by collagenase enzyme activity that degrades collagen. In general, at 80 years of age, the skin thickness is reduced by 65% compared to 20 years, and this phenomenon is known to be closely related to the wrinkle formation of the skin.

Second, elastin fibers form crosslinks with collagen and are important skin components for the production of wrinkles involved in skin elasticity. Deficiency and aggregation of elastin fibers and a dramatic increase in the activity of elastin, an elastin degrading enzyme, have been found to be one of the causes of skin wrinkles. Elastase is the only enzyme that can break down elastin and its inhibition is known to fundamentally reduce skin wrinkle improvement. Decreased skin elasticity is caused by decreased production and destruction of elastin due to aging and weakening of skin cells (fibroblasts) caused by ultraviolet rays. Elastin degrading enzyme is expressed in the skin by ultraviolet rays, etc. It causes a decrease in skin elasticity.

Third, free radicals generated by ultraviolet rays in sunlight are highly reactive reactive oxygen species, which cause cell damage and death by oxidizing or denaturing key biological components such as sugars, proteins, DNA, and cell membrane lipids. In particular, it has been found to be the biggest cause of cell aging in the skin. Free radicals are elements or molecules with one or more unpaired electrons that occur due to oxygen reduction, UV irradiation, or phagocytosis during the breathing process of the living body. Properties to cause cell damage.

Many cosmetic companies and research institutes are using moisturizers, anti-inflammatory agents, elastin, collagen and nutritional ingredients in cosmetics to reduce wrinkles and delay skin aging, but there is a limit to delaying aging. Therefore, there is a need for an anti-wrinkle agent through the blockade of elastin and collagen reduction to maintain skin components and elasticity and promoting collagen and elastin production. In addition, there is a need for a raw material having a function of inhibiting and eliminating the production of free radicals that affect skin cell damage.

Currently, wrinkle improvement cosmetics are known as retinoids, adenosine, animal placental protein, chlorella extract and the like. The most well known retinol promotes collagen synthesis and inhibits elastase enzyme, but it is unstable and has limited use due to safety issues such as irritation and redness when applying skin. Chlorella extract is practically a wrinkle It is difficult to expect improvement effects. Therefore, there is an urgent need to develop a material having safety and stability, preventing and promoting collagen and elastin, and at the same time, having a free radical generation inhibitory and scavenging effect.

In view of this reality, the present inventors searched for substances that are fundamentally inhibiting the decrease in skin elasticity due to conventional elastin deterioration and destruction. In addition to the inhibitory substance, the present invention was found to improve the apparent skin elasticity and improve various skin aging phenomena even in the test results applied directly to human skin.

Accordingly, an object of the present invention is to provide an anti-wrinkle cosmetic composition containing haejucho extract having an elastase inhibitory effect.

In order to achieve the above technical problem, the present invention relates to a cosmetic composition for improving skin wrinkles containing a hagocho extract as an active ingredient, containing 0.0001 to 10.0% by weight of the hagocho extract as a dry weight based on the total weight of the cosmetic composition It is characterized by. The present invention is characterized by strongly inhibiting the enzyme involved in wrinkle formation, that is, the elastase activity, by incorporating the hydrangea extract into the cosmetic.

Hereinafter, the cosmetic for improving skin wrinkles according to the present invention will be described in detail.

Prunella vulgaris L. Is a perennial herb that grows in most places in China . It has been reported to lower blood pressure and antibacterial activity. In Chinese medicine, narcosis, acute mastitis, eye pain, dizziness, orbital pain, joint pain, It is used for the treatment of hemoptysis and lobster. Triterpenoid saponin, oleanolic acid, rutin, tannin, essential oils and alkaloids have been reported as main ingredients.

It has not been known that the above-mentioned Hagocho was previously known to have anti-wrinkle and anti-aging effects, and furthermore, the extract of the natural plant was not applied to the anti-wrinkle effect.

The present invention will be described in more detail as follows. It is used as a sample of the present invention by purchasing Hagocho on herbal medicines in the general market.

The present inventors evaluated the activity of inhibiting the activity of elastase enzyme that promotes wrinkle formation by using the extract of Prunella vulgaris, and it was found that the extract of Prunella vulgaris showed a very potent suppression of elastinase. Therefore, when the natural extract of Hachocho extract is applied to cosmetics such as skin ointments, essences, creams, etc., it can exhibit a strong skin wrinkle suppression effect without any side effects.

Higocho can be easily purchased on the market, and its extract can be, for example, after heating and extracting Higocho with water or an organic solvent such as anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, etc. It can be obtained by drying or spray drying. To obtain a high purity extract, the extract may be extracted one or more times in the same manner.

Hagocho extract obtained as described above can be used in a variety of cosmetics such as skin ointment or lotion. The hydrangea extract contained in the cosmetic preparation according to the present invention preferably contains 0.0001 to 10.0% by weight based on the total weight of the cosmetic composition as a dry weight, more preferably 0.0005 to 10.0% by weight. If the content is less than 0.0001% by weight, it is difficult to expect the inhibition of the elastase activity, and if it exceeds 10.0% by weight, no apparent effect is expected as the increasing content.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Hagocho Extraction Example

Extraction Example  One

Purified 100 g of commercially available pulverized crushed powder was added to 3 l of 80% methanol, boiled and extracted for 3 hours in a reflux extractor with a cooling capacitor. Subsequently, the resultant was filtered through a 100 mesh filter cloth, and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to obtain 5.5 g of dry extract.

Extraction Example  2 ~ 6

Except for changing the solvent according to the description in Table 1 instead of 80% methanol was carried out in the same manner as in Extraction Example 1.

menstruum Dry extract (g) Extraction Example 1 80% methanol 5.5 Extraction Example 2 Purified water 5.1 Extraction Example 3 95% ethanol 5.6 Extraction Example 4 70% acetone 5.2 Extraction Example 5 Ethyl acetate 3.7 Extraction Example 6 chloroform 3.3

Example  1-6

Elastase activity inhibitory activity was evaluated for each extract obtained according to the extraction examples 1 to 6. Elastase used 0.2mU of elastays extracted from human leukocytes and used a final substrate of 0.7mM as the substrate MeOSuc-Ala-Ala-Pro-Val-pNA. As samples, the extracts of the extraction examples 1 to 6 were each tested at a concentration of 50 ug / ml. The final volume of the reaction solution was 300ul and proceeded at 37 ° C. The reaction was performed for 10 minutes in kinetics mode, 405nm using Biotek Powerwave x 340 microplate reader. Elastase inhibitory effect for each sample was calculated by the following equation, the results are shown in Table 2 below.

Elastase Inhibition Effect (%) = [1- Experimental Absorbance 405nm / Control Absorbance 405nm] x 100

sample  Experimental concentration (ug / ml) Elastase Inhibition Effect (%) Control group (no addition) - - Example 1 50 45 Example 2 50 48 Example 3 50 51 Example 4 50 47 Example 5 50 45 Example 6 50 49

Referring to Table 2, it can be seen that each sample obtained according to the extraction examples 1 to 6 has a very strong activity inhibitory activity against the elastase enzyme compared to the control (Examples 1 to 6).

Example  7-10

In the same manner as in Examples 1 to 6, the effect of inhibiting the elastase according to the concentration change of the extract of Extraction Example 3 was shown in Table 3 below.

sample Experimental concentration (ug / ml) Elastase Inhibition Effect (%) Control group (no addition) - - Example 7  Extract of Extraction Example 3 50 52 Example 8 100 60 Example 9 200 85 Example 10 500 92

As shown in the results of Table 3, it can be seen that the higher the concentration of the Hagocho extract, the greater the ability to inhibit the elastase activity.

In the following, the extract of Hachocho extract is added to the external skin ointment, cream, supple cosmetics, nourishing cosmetics, packs, essences to prepare cosmetics, and look at the effect of improving the wrinkles appeared by prescribing them to the test subjects.

Production Example  One

Hagocho extract obtained from Extraction Example 3 was added as an active ingredient, and an external skin ointment was prepared using the ingredients and contents shown in Table 4 below.

Comparative example  One

Without adding a plant extract, the skin external ointment was prepared with the ingredients and contents shown in Table 4 below.

Composition (% by weight) Production Example Comparative example One One Plant extract Hagocho extract One - Diethyl sebacate 8 8 Prepayment 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Quantity Quantity vaseline to 100 to 100

Production Example  2

Hagocho extract obtained from Extraction Example 3 was added as an active ingredient, and a cream was prepared using the ingredients and contents shown in Table 5 below.

Comparative example  2

Cream was prepared with the ingredients and contents shown in Table 5 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 2 2 Plant extracts Hagocho extract 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Quantity Quantity incense Quantity Quantity Purified water to 100 to 100

Production Example  3

Hagocho extract obtained from Extraction Example 3 was added as an active ingredient, respectively, and a softened longevity was prepared using the ingredients and contents shown in Table 6 below.

Comparative example  3

Without adding a plant extract, softening water was prepared with the ingredients and contents shown in Table 6.

Composition (% by weight) Production Example Comparative example 3 3 Plant extracts Hagocho extract 1.0 - ethanol 10 10 Polylauric acid polyoxyethylene sorbitan One One Methyl paraoxybenzoate 0.2 0.2 glycerin 5 5 1,3-butylene glycol 6 6 incense Quantity Quantity Pigment Quantity Quantity Purified water to 100 to 100

Production Example  4

Hagocho extract obtained from Extraction Example 3 was added as an active ingredient, and nutritional longevity was prepared using the ingredients and contents shown in Table 7 below.

Comparative example  4

No nutrient extract was prepared using the ingredients and contents shown in Table 7 below without adding a plant extract.

Composition (% by weight) Production Example Comparative example 4 4 Plant extracts Hagocho extract One - vaseline 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Quantity Quantity incense Quantity Quantity Purified water to 100 to 100

Production Example  5

Hagocho extract obtained from Extraction Example 3 was added as an active ingredient, and a pack was prepared with the ingredients and contents shown in Table 8 below.

Comparative example  5

Packs were prepared with the ingredients and contents shown in Table 8 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 5 5 Plant extracts Hagocho extract One - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Quantity Quantity Pigment Quantity Quantity Purified water to 100 to 100

Production Example  6

Hagocho extract obtained from Extraction Example 3 was added as an active ingredient, and an essence was prepared according to the ingredients and contents shown in Table 9 below.

Comparative example  6

Essence was prepared with the ingredients and contents shown in Table 9 below without the addition of plant extracts.

Composition (% by weight) Production Example Comparative example 6 6 Plant extracts Hagocho extract 5 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Quantity Quantity Purified water to 100 to 100

The method used to verify the skin wrinkle improvement effect by the external skin ointment, cream, supple cosmetics, nutrient cosmetics, pack, essence prepared as described above is as follows.

Wrinkle improvement for 15 subjects with facial wrinkles aged 35 to 45 years with respect to the essence (Preparation Example 6) of the following composition by applying the anti-wrinkle effect on cosmetics containing the natural herbal extract of the present invention The effect was compared and evaluated.

Preparation 6 essence was used on the left side of the subject, and Comparative Example 6 essence was used twice on the right side for 3 months after face wash in the morning and evening. Compared with Comparative Example 6, the degree of wrinkle improvement of Preparation Example 6 was visually determined, and Preparation Example 6 compared the Comparative Example 6 with the "improving effect", "with wrinkle improvement effect", "not sure", It was evaluated in four stages of "none", the results are shown in [Table 10].

Experimental substance Distinct Effects (persons) Wrinkle improvement effect (person) I don't know (person)  None (persons) Preparation Example 6 3 9 2 One

As shown in Table 10, the cosmetic composition containing the vinegar extract prepared according to Preparation Example 6 showed a skin wrinkle improvement effect of at least 12 or more of 15 subjects, and did not show any side effects in the skin.

In addition, in order to apply the skin elasticity-promoting effect on the cosmetics containing the natural herbal extract of the present invention to human skin, 30 healthy adults with the essences of Preparation Example 6 and Comparative Example 6 in a constant temperature and humidity room of 22 ℃ and 50% relative humidity After applying for 4 weeks on the neck, the elasticity before and after the chart was measured to compare the skin elasticity improvement effect.

Preparation 6 essence was used on the left side of the subject's neck and Comparative Example 6 essence was used twice a day for 4 weeks after washing the face in the morning and evening. Then, the degree of improvement of elasticity at each site was measured using a skin elasticity measuring instrument (Cutometer, C + K, Germany). The degree of improvement used Ur / Uf as a parameter.

Elasticity improvement (%, n = 30) Preparation Example 6 22.07 Comparative Example 6  3.47

Skin wrinkle improvement cosmetics containing the extract of haejucho of the present invention significantly reduced the activity of the elastase involved in the generation of wrinkles, and confirmed the wrinkle improvement effect and elasticity improvement effect through clinical evaluation.

Claims (3)

Cosmetic composition for improving skin wrinkles containing the Hagocho extract as an active ingredient. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the hachocho extract is contained 0.0001 to 10.0% by weight based on the total weight of the composition as a dry weight. The cosmetic composition for improving skin wrinkles according to claim 1 or 2, wherein the extract of Hagocho is extracted with one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate and acetone. Composition.