KR100963093B1 - Method for extracting using Himalayan Glacial Water and the extracts thereof, and cosmetic composition containing nanoparticles encapsulated the extracts - Google Patents

Abstract

The present invention provides a solvent extraction method for extracting the active ingredient using the Himalayan natural glacial water that has been deposited for a long time, and extracts thereof, and nano-emulsified particles of the extract containing various minerals or active ingredients using nanoemulsification technology And by containing this as an active ingredient to provide a cosmetic composition that greatly improved the transdermal absorption.

Glacial water, minerals, nanoemulsification technology, solvent extraction method, cosmetic composition

Description

Method for extracting using Himalayan Glacial Water and the extracts etc, and cosmetic composition containing nanoparticles encapsulated the extracts}

The present invention provides a solvent extraction method for extracting the active ingredient using the Himalayan natural glacial water that has been deposited for a long time, and extracts thereof, and nano-emulsified particles of the extract containing various minerals or active ingredients using nanoemulsification technology And it is to provide a cosmetic composition that greatly improved the percutaneous absorption by containing this as an active ingredient.

Glacial water is formed by melting ice in high mountains or in polar regions for tens of millions of years without melting the snow, which is transformed into hard ice under pressure.

At present, glacial water is formed in the polar regions and alpine regions, which are the best clean areas on earth, and symbolizes the health and pure vitality of nature. The representative glacier water regions are Bilkabamba in Ecuador, Abkhazia in the Caucasus, and Hunza in the Himalayas.

Since these glaciers are difficult to breed by microorganisms and pathogens, have a high ratio of hexagonal water, and are rich in minerals, the area where glacier water comes from is known as a longevity village.

These minerals are responsible for osmotic pressure, acid and alkali equilibrium, wound recovery and metabolic functions in the body, and play an important role in oxygen transfer and cellular respiration.

Therefore, research on a new solvent extraction method that can increase the transdermal absorption of minerals is being actively conducted.

Looking at the process of the conventional solvent extraction method, water; Organic solvents such as ethanol, methanol, butanol, ether, ethyl acetate and chloroform; Alternatively, the organic solvent containing water was added and left at room temperature for one day to extract the extract twice or more times to obtain an extract. After filtering the extract, the filtrate was concentrated in a vacuum concentrator to obtain a primary product.

Water and organic solvents such as ethanol, methanol, butanol, ether, ethyl acetate, and chloroform are added to the obtained product, the mixture is stirred at room temperature for at least 2 hours, and then allowed to stand to separate layers. After separating the layers, the water layer is removed, and then an organic solvent is further added. The above process is repeated two or more times, sufficiently washed and filtered, and then the filtrate is dried in a vacuum oven to obtain a desired extract.

The conventional solvent extraction method has emerged a need for a new solvent extraction method due to the safety problems for the skin due to the remaining organic solvent, the formulation and stability problems when formulated in cosmetics.

There is also an increasing demand for the development of new solvent extraction methods that can double the absorption of active ingredients such as minerals while increasing safety.

 The present inventors have tried to find a solvent that can replace the organic solvent in order to solve problems such as skin safety due to the remaining organic solvent, the existing active ingredient is extracted using Himalayan glacier water instead of water or organic solvent In this case, the skin stability is excellent and the absorption of the active ingredient can be doubled, and the extract thus obtained is collected into the nano-emulsified particles using nanotechnology, and then contained, thereby providing a cosmetic composition having a greatly improved transdermal absorption rate. The present invention was completed.

Accordingly, it is an object of the present invention to provide a solvent extraction method and its extract, and a cosmetic composition containing the extract as an active ingredient, which has excellent skin stability and can double absorption of minerals or active ingredients.

In order to achieve the above object, the present invention prepares an extract containing various minerals or active ingredients and nano-emulsified particles including the same by using a solvent extraction method and nanoemulsification technology using Himalayan natural glacial water that has been deposited for a long time, and an active ingredient thereof. Provided is a cosmetic composition that greatly improves transdermal absorption.

In the present invention, by extracting the active ingredient using the Himalayan glacier water rich in natural minerals, the skin safety was excellent, and the effect of the active ingredient was improved, and the extract was also nano-emulsified using a skin-friendly emulsifier and nanoemulsification technology. By stabilizing in the particles to maximize the transdermal absorption of the cosmetic composition comprising the nano-emulsified particles.

Hereinafter, the present invention will be described in more detail.

Himalayan glacial water, also called "Hunja Water", has a unique structure in which nano-sized silica forms colloids in water, and has a low surface tension to facilitate the absorption of active ingredients into the human body or skin. In addition, the Himalayan glacier water is trapped inside a cage-like water molecule that resembles a hemispherical dome that can trap large amounts of hydrogen, and these water molecules have small amounts of colloidal inorganic particles with high zeta potential. have. The shape of the water molecules formed at this time is very similar to the shape of water molecules found in biological tissues, and is known to be very different from the shape of water molecules found in ordinary mineral water or tap water.

The present invention provides a solvent extraction method using the glacial water. Solvent extraction using glacial water according to the present invention comprises the following steps:

1) adding glacial water to the active ingredient and left for one day at room temperature to extract the extract twice or more, and then filtrate the filtered filtrate in a vacuum concentrator to obtain a primary product; And

2) adding glacial water to the obtained product again, stirring at room temperature for 2 hours or more, sufficiently washing and filtering, and drying in a vacuum oven to obtain a extract.

The glacier water used in the present invention passes through natural filtration devices such as granite, gravel, and fine particles as the ice melts itself, and the filtered aquifer is extracted several kilometers from the underground, and the aquifer layer deep in the underground is a natural protection device. It is obtained through natural filtration that is not contaminated with any material by the granite layer. The glacial water is pre-filtered. Therefore, the solvent extraction method using glacial water according to the present invention is very simplified in the solvent extraction method according to the present invention, unlike the conventional solvent extraction method using a solvent extraction method using an organic solvent, it is characterized in that the easy to obtain the extract to be.

The solvent extraction method using the glacial water is a quick skin absorption of minerals based on glacial water containing zinc, copper, vanadium and manganese, in addition to sodium, magnesium, calcium and potassium, which are active minerals, and glacial water that is difficult to reproduce microorganisms or pathogens. Since it has the advantage of purifying action due to toxin removal action, it can be used regardless of user's skin type, and furthermore, to provide a cosmetic composition that can be used safely even for infants or people with skin problems such as atopy. It is characterized by.

The active ingredient which can be extracted by the solvent extraction method according to the present invention is not particularly limited, and specifically, beta-1,3-glucan, papain enzyme, polyphenol, saponin, adenosine, vitamin C (ascorbic acid), arbutin ), Niacinamide and acetylglucosamine.

The beta-1,3-glucan extracted by the solvent extraction method of the present invention can extract glucan with high purity of 98% or more, which is obtained by culturing Schizopyllum commune mycelium, which prevents skin aging and damages skin. It works. In addition to the skirt mushroom, beta-1,3-glucan can be obtained through solvent extraction using glacial water according to the present invention from Ganoderma lucidum mushroom, Shiitake mushroom, shiitake mushroom, yeast and barley.

In addition, the papain enzyme of the active ingredient may be extracted from the fruit or tree of papaya, polyphenols are red or purple anthocyanin pigments such as green tea, coffee, strawberries, eggplant, grapes, red beans, red wine, black beans or cacao Can be extracted from.

Saponins can be extracted from beans, ginseng or hemp, adenosine can be extracted from plants such as stone parsley, vitamin C (ascorbic acid) can be extracted from bokbunja, persimmon leaves, rosehips, grapefruit or bell peppers, and arbutin It can be extracted from bilberry, niacinamide can be extracted from potato or avocado, and acetylglucosamine can be extracted from Phellodendron amurense RUPR and Phellodendron. chinense Bark of SCHNEID) can be extracted from the dried medicinal herb.

In the present invention, by applying nanoemulsification technology to the extract (active ingredient) extracted using the glacial water can be collected into fine emulsified particles or liposomes, through this process can increase the transdermal absorption of minerals contained in the glacial water Will be. The extract extracted using the glacial water is contained in an amount of 0.001 to 50% by weight based on the total weight of the emulsified particles or liposomes. If the extract content is less than 0.001% by weight, there is a problem that the active ingredient is difficult to exert an effect in the formulation, and when the content exceeds 50% by weight, the formulation is difficult. In the present invention, nanoemulsified particles containing the extract may be prepared using a conventional high pressure emulsification method well known in the art at a pressure of 500 to 1000 bar as a nanoemulsification technique.

The emulsified particles according to the present invention have a particle diameter of about 1000 nm to 30 nm, preferably about 300 nm to 50 nm. In the nano-emulsified particles or liposomes of the present invention, the area of contact with the skin is relatively increased, thereby increasing the percutaneous absorption possible area. In addition, the emulsion particles according to the present invention facilitate the absorption and diffusion into the intercellular lipids in consideration of the fact that the gap between the intercellular lipids of the stratum corneum is about 50 nm and that the emulsion membrane of the emulsion particles has flexibility. . That is, emulsified particles having an average particle diameter of 300 nm to 50 nm prepared by nanoemulsification technology increase the contact area with the skin; Penetration and diffusion into intercellular lipids; And through three routes of extracts extracted by glacial water solvent extraction method, it increases the percutaneous absorption rate of the emulsified particles themselves and the extract inside the emulsified particles.

On the other hand, the emulsified particles according to the present invention may further contain lecithin as an emulsifier together with the extract, the content of the lecithin is 0.5 to 5% by weight, preferably 2 to 4% by weight relative to the total weight of the emulsified particles. The content of lecithin is an effective amount to make a stable formulation in formulating the cosmetic composition. The components of the lecithin include unsaturated choline compounds such as phosphatidylcholine, lysophosphatidylcholine and phosphatidylethanolamine, and hydrogenated forms thereof.

In addition, the emulsified particles according to the present invention can be widely used a variety of nonionic surfactants as co-emulsifiers, and preferably include a fatty alcohol mixed surfactant or a polymeric surfactant. The content of the co-emulsifier is used in a ratio of 0.1 to 5 times, preferably 0.5 to 2 times the weight of the lecithin, depending on the weight and components of the lecithin used.

In another aspect, the present invention provides a cosmetic composition containing the emulsified particles or liposomes collected by the extract extracted using the glacial water. The cosmetic composition according to the present invention contains the emulsified particles or liposomes in an amount of 0.001 to 50% by weight based on the total weight of the composition. When the content of the emulsion particles or liposomes is less than 0.001% by weight, there is a problem that the active ingredient hardly exerts an effect in the formulation, and when the content exceeds 50% by weight, the formulation is difficult.

The cosmetic composition according to the present invention is not particularly limited in its formulation. For example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, It may be formulated as a body essence, makeup base, foundation, hair dye, shampoo, rinse, body cleanser, toothpaste, or mouthwash, and the like, or may be formulated as a cosmetic such as skin whitening cosmetics or ointments and patches.

The present invention is explained in more detail with reference to the following examples, but the present invention is not limited only to these examples.

Example 1 Preparation of Beta-1,3-Glucan Extract Using Glacial Water

4L of Himalayan glacier water was added to 1 kg of dried mycelium mycelium and extracted by standing at room temperature for one day. The above process was repeated twice to obtain an extract. The extract was filtered and the filtrate was concentrated in a vacuum concentrator to obtain 400 g of the obtained product. 1 g of glacier water was added to 400 g of the obtained product, the mixture was stirred at room temperature for 2 hours or more, then sufficiently washed and filtered. Dried to obtain the desired extract.

Comparative Example 1

4 kg of ethanol was added to 1 kg of dried mycelium mycelium mycelium, and the extraction process was repeated two or more times at room temperature for one day. The extract was filtered, and the filtrate was concentrated in a vacuum concentrator to obtain 400 g of the first obtained product. Got it. 1 L of water and ethanol were added to the obtained product, and the mixture was stirred at room temperature for 2 hours or more, allowed to stand, and the layers were separated. Then, 1 L of ethanol was further added thereto. The above process was repeated two or more times, sufficiently washed and filtered, and the filtrate was dried in a vacuum oven to obtain a beta-1,3-glucan extract.

Example 2 Preparation of Nanoemulsified Particles

After mixing 4 g hydrogenated lecithin, 4 g PEG-5 rapeseed sterol, 5 g polyethylene glycol, and 5 g pentylene glycol, a solution of 1 g of beta-1,3-glucan extract prepared in Example 1 was dissolved in 10 g of ethanol. After dissolving in the mixed solution and heating to 70-75 ° C, completely dissolving, mixing with pre-heated water-part (65.95g distilled water, 5g glycerin and EDTA 0.05g), pre-emulsifying with a general homomixer (3,000-6,000 for 3 minutes) rpm), and emulsified at 800 Bar / 3cycles using a high pressure emulsifier.

Comparative Example 2

4 g of hydrogenated lecithin, 4 g of PEG-5 rapeseed sterol, 5 g of polyethylene glycol, 5 g of pentylene glycol, and 10 g of ethanol were completely dissolved by heating to 70-75 ° C., and then pre-heated water part (66.95 g of distilled water, glycerin 5). And EDTA 0.05 g), pre-emulsified with a common homomixer (3,000-6,000 rpm for 3 minutes), and emulsified at 800 Bar / 3cycles using a high pressure emulsifier.

Comparative Example 3

It was prepared in the same manner as in Example 2 except for using the beta-1,3-glucan extract prepared in Comparative Example 1 instead of the beta-1,3-glucan extract prepared in Example 1.

Test Example 1 Collagen Biosynthesis Efficacy on Human Skin Cells

Human fibroblasts were cultured in a 24-hole plate incubator and then replaced with a medium containing each composition having the concentrations shown in Table 2, followed by 3 days of culture and 0.5 ml of DMEM medium containing 10% fetal calf serum per well. After addition each 10 [ mu] Ci of L [2, 3, 4, 5-3H] -proline was added. After 24 hours, the media and cells in each well were scraped and washed in 5% Trichloroacetic acid (TCA) solution, and then dispensed into two test tubes, and one type I collagenase in one test tube. 1 unit / μl of (type I collagenase) was added and incubated at 37 ° C. for 90 minutes, and other test tubes were stored at 4 ° C. Thereafter, 0.05 ml of 50% trichloroacetic acid was added to all test tubes, and the mixture was allowed to stand for 4 to 20 minutes, followed by centrifugation at 12,000 rpm for 10 minutes, respectively, and the supernatants and precipitates were transferred to a liquid scintillation counter (LSC). CPM (counts per minute) values were obtained, and collagen biosynthesis values (RCB; Relative Collagen Biosynthesis) were obtained for the control group and the experimental group based on Equation 1 below. The results are shown in Table 1 below to obtain a comparison value with the control group 100.

RCB = [collagen CPM / {(total collagen-collagen CPM) × 5.4 + collagen CPM}] × 100

Collagen biosynthesis rate (RCB) over time (%) Concentration (ppm) Control Example 1 Example 2 10 100 120 106 One 100 105 101

From the results of Table 1, it can be seen that when the beta-1,3-glucan extract extracted with Himalayan glacial water is prepared using nanoemulsification technology, collagen biosynthesis increases concentration-dependently.

[Formulation Example 1 and Comparative Formulation Examples 1 to 3]

As shown in Table 2, the nutrient cosmetics of Formulation Example 1 and Comparative Formulation Examples 1 to 3 were prepared.

Furtherance Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Example 2 10.0 - - - Comparative Example 2 - 10.0 - - Comparative Example 3 - - 10.0 - Cetylethylhexanoate 5.0 5.0 5.0 5.0 Cetostearyl alcohol 1.0 1.0 1.0 1.0 Lipophilic Monostearic Acid Stearate 0.8 0.8 0.8 0.8 Squalane 2.0 2.0 2.0 2.0 Polysorbate 60 1.5 1.5 1.5 1.5 Solbitan Sesquioleate 0.5 0.5 0.5 0.5 Polyethylene glycol 5.0 5.0 5.0 5.0 Triethanolamine 0.2 0.2 0.2 0.2 Carboxy Vinyl Polymer 0.2 0.2 0.2 0.2 antiseptic a very small amount a very small amount a very small amount a very small amount Pigment a very small amount a very small amount a very small amount a very small amount Spices a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance

Test Example 2 Collagen Biosynthesis Efficacy in Animals

Nutritional longevity having the composition of Formulation Example 1 and Comparative Formulation Examples 1 to 3 on the back of a hairless mouse to confirm whether the collagen biosynthesis efficacy is enhanced when beta-1,3-glucan extracted with glacial water according to the present invention is added Was applied for a week and then biopsied to perform collagen immunohistostaining. The result was calculated | required by the comparative value which made comparative formulation example 3 which is an untreated group 100, and is shown in Table 3.

Test substance Collagen Biosynthesis (%) Formulation Example 1 135 Comparative Formulation Example 1 105 Comparative Formulation Example 2 128 Comparative Formulation Example 3 100

As can be seen from the results of Table 3, it was observed that the collagen biosynthesis increased by about 28.6% and 5.5%, respectively, in the group coated with Formulation Example 1 than the group coated with Comparative Formulations 1-2. Through this, the present invention was confirmed that the collagen biosynthesis effect is increased by increasing the transdermal absorption rate when using beta-1,3-glucan extract using Himalayan glacial water.

Test Example 3 Measurement of Skin Elasticity Improvement Effect in Human Body

The skin elasticity improving effect of the nutrient cosmetics prepared in Table 2 was measured. After dividing 20 healthy women over 30 years old into two groups at the temperature of 24 ~ 26 ℃ and 75% humidity, apply facial lotion of Formulation Example 1 and Comparative Formulation Example 2 twice a day for 12 weeks on the face of women in each group. Skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany).

The results were obtained from R8 [R8 (left) -R8 (right)] of Cutometer SEM 575 and are shown in Table 4 below. The R8 value indicates the nature of skin viscoelasticity.

Test substance Skin elasticity effect Formulation Example 1 0.33 Comparative Formulation Example 2 0.10

As can be seen from the results of Table 4, the group coated with the material of Formulation Example 1 containing according to the present invention further increased skin elasticity compared to the group applied with Comparative Formulation Example 2.

Separately, at the end of the test, the subjects were asked to complete the questionnaire, and the subjective efficacy evaluation was conducted simultaneously with the mechanical evaluation. The results are shown in Table 5 below.

Applicator Respondents (Personal) Very good Good usually Inadequate Formulation Example 1 3 6 One 0 Comparative Formulation Example 2 One 3 3 3

In addition, the survey also confirmed that the skin elasticity was improved when the formulation example 1 containing beta-1,3-glucan extract extracted by the Himalayan glacial water solvent extraction method according to the present invention.

Claims (8)

Extraction method for extracting active ingredients from plants using glacial water as an extraction solvent, 1) adding glacial water to the plant and leaving it at room temperature for one day to extract the extract twice or more, and then filtrate the filtrate filtered in a vacuum concentrator to obtain a primary product; And 2) adding glacial water to the obtained product again, stirring at room temperature for 2 hours or more, sufficiently washing, filtering, and drying in a vacuum oven to obtain a extract; Extraction method characterized in that it comprises a. delete The method of claim 1, wherein the plant is a skirt mushroom, Ganoderma lucidum mushroom, spiritual mushroom, shiitake mushroom, mycobacterium fungus, yeast, barley, papaya, green tea, coffee, strawberry, eggplant, grapes, red beans, black beans, cacao, beans, ginseng, Extraction method characterized in that at least one member selected from the group consisting of hemp, parsley, bokbunja, persimmon leaves, rose hips, grapefruit, bell pepper, bilberry, potato, avocado and yellow white. The method of claim 1, wherein the active ingredient is beta-1,3-glucan, papain enzyme, polyphenol, saponin, adenosine, vitamin C (ascorbic acid), arbutin, niacinamide and acetylglucosamine (acetylglucosamine) extraction method characterized in that at least one selected from the group consisting of. Nanoemulsified particles comprising the active ingredient and lecithin extracted by the method according to claim 1 and having a particle size of 1000 to 30nm. The nanoemulsified particles according to claim 5, wherein the content of the active ingredient is 0.001 to 50% by weight based on the total weight of the nanoemulsified particles. The nanoemulsified particles according to claim 5, wherein the content of the lecithin is 0.5 to 5% by weight based on the total weight of the nano emulsified particles. A cosmetic composition comprising the nanoemulsified particles according to any one of claims 5 to 7 as an active ingredient.