WO2009091183A2 - Method for extracting using glacial water and the extract thereof, and cosmetic composition comprising namoemulsion particles having the extract encapsulated or the glacial water. - Google Patents
Method for extracting using glacial water and the extract thereof, and cosmetic composition comprising namoemulsion particles having the extract encapsulated or the glacial water. Download PDFInfo
- Publication number
- WO2009091183A2 WO2009091183A2 PCT/KR2009/000200 KR2009000200W WO2009091183A2 WO 2009091183 A2 WO2009091183 A2 WO 2009091183A2 KR 2009000200 W KR2009000200 W KR 2009000200W WO 2009091183 A2 WO2009091183 A2 WO 2009091183A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- skin
- water
- glacier water
- glacier
- active ingredient
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 135
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 125
- 239000000284 extract Substances 0.000 title claims abstract description 44
- 239000002245 particle Substances 0.000 title claims abstract description 35
- 239000002537 cosmetic Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000004480 active ingredient Substances 0.000 claims abstract description 32
- 239000007908 nanoemulsion Substances 0.000 claims abstract description 25
- 102000011782 Keratins Human genes 0.000 claims abstract description 16
- 108010076876 Keratins Proteins 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 15
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 239000000047 product Substances 0.000 claims description 31
- 229920002498 Beta-glucan Polymers 0.000 claims description 19
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 10
- 235000010445 lecithin Nutrition 0.000 claims description 8
- 239000000787 lecithin Substances 0.000 claims description 8
- 229940067606 lecithin Drugs 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 6
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 claims description 6
- 230000003020 moisturizing effect Effects 0.000 claims description 6
- 241000222481 Schizophyllum commune Species 0.000 claims description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 3
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims description 3
- DUKURNFHYQXCJG-UHFFFAOYSA-N Lewis A pentasaccharide Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(C)=O)C(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)OC1CO DUKURNFHYQXCJG-UHFFFAOYSA-N 0.000 claims description 3
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 claims description 3
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 229960005305 adenosine Drugs 0.000 claims description 3
- 229960000271 arbutin Drugs 0.000 claims description 3
- 229950006780 n-acetylglucosamine Drugs 0.000 claims description 3
- 235000005152 nicotinamide Nutrition 0.000 claims description 3
- 229960003966 nicotinamide Drugs 0.000 claims description 3
- 239000011570 nicotinamide Substances 0.000 claims description 3
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 3
- 235000013824 polyphenols Nutrition 0.000 claims description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 3
- 229930182490 saponin Natural products 0.000 claims description 3
- 150000007949 saponins Chemical class 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 241001327634 Agaricus blazei Species 0.000 claims description 2
- 244000139693 Arctostaphylos uva ursi Species 0.000 claims description 2
- 235000012871 Arctostaphylos uva ursi Nutrition 0.000 claims description 2
- 240000004160 Capsicum annuum Species 0.000 claims description 2
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 claims description 2
- 240000006432 Carica papaya Species 0.000 claims description 2
- 235000009467 Carica papaya Nutrition 0.000 claims description 2
- 240000000560 Citrus x paradisi Species 0.000 claims description 2
- 240000007154 Coffea arabica Species 0.000 claims description 2
- 235000002723 Dioscorea alata Nutrition 0.000 claims description 2
- 235000007056 Dioscorea composita Nutrition 0.000 claims description 2
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 claims description 2
- 235000005362 Dioscorea floribunda Nutrition 0.000 claims description 2
- 235000004868 Dioscorea macrostachya Nutrition 0.000 claims description 2
- 235000005361 Dioscorea nummularia Nutrition 0.000 claims description 2
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 claims description 2
- 235000011511 Diospyros Nutrition 0.000 claims description 2
- 244000236655 Diospyros kaki Species 0.000 claims description 2
- 240000009088 Fragaria x ananassa Species 0.000 claims description 2
- 241001489090 Ganoderma japonicum Species 0.000 claims description 2
- 240000005979 Hordeum vulgare Species 0.000 claims description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 2
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 claims description 2
- 240000000599 Lentinula edodes Species 0.000 claims description 2
- 235000001715 Lentinula edodes Nutrition 0.000 claims description 2
- 241000775369 Musineon Species 0.000 claims description 2
- 240000004371 Panax ginseng Species 0.000 claims description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 2
- 244000025272 Persea americana Species 0.000 claims description 2
- 235000008673 Persea americana Nutrition 0.000 claims description 2
- 235000002770 Petroselinum crispum Nutrition 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 241001618264 Rubus coreanus Species 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims description 2
- 244000061458 Solanum melongena Species 0.000 claims description 2
- 235000002597 Solanum melongena Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 claims description 2
- 244000299461 Theobroma cacao Species 0.000 claims description 2
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 claims description 2
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 claims description 2
- 240000001417 Vigna umbellata Species 0.000 claims description 2
- 235000011453 Vigna umbellata Nutrition 0.000 claims description 2
- 235000001046 cacaotero Nutrition 0.000 claims description 2
- 235000016213 coffee Nutrition 0.000 claims description 2
- 235000013353 coffee beverage Nutrition 0.000 claims description 2
- 235000004879 dioscorea Nutrition 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 235000008434 ginseng Nutrition 0.000 claims description 2
- 235000009569 green tea Nutrition 0.000 claims description 2
- 235000017709 saponins Nutrition 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 240000005717 Dioscorea alata Species 0.000 claims 1
- 235000016623 Fragaria vesca Nutrition 0.000 claims 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 241000972673 Phellodendron amurense Species 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 241000219094 Vitaceae Species 0.000 claims 1
- 235000021021 grapes Nutrition 0.000 claims 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 11
- 239000011707 mineral Substances 0.000 abstract description 11
- 230000036548 skin texture Effects 0.000 abstract description 6
- 238000009472 formulation Methods 0.000 description 90
- 210000003491 skin Anatomy 0.000 description 73
- 230000000052 comparative effect Effects 0.000 description 51
- 239000006071 cream Substances 0.000 description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- 239000000839 emulsion Substances 0.000 description 16
- 230000000694 effects Effects 0.000 description 12
- 230000001965 increasing effect Effects 0.000 description 11
- 210000000434 stratum corneum Anatomy 0.000 description 11
- 206010013786 Dry skin Diseases 0.000 description 10
- 210000000245 forearm Anatomy 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 9
- 239000003960 organic solvent Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 8
- 239000004615 ingredient Substances 0.000 description 8
- 238000000638 solvent extraction Methods 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000036570 collagen biosynthesis Effects 0.000 description 6
- 239000002502 liposome Substances 0.000 description 6
- 239000006210 lotion Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000037394 skin elasticity Effects 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 229940008099 dimethicone Drugs 0.000 description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 description 5
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 235000011187 glycerol Nutrition 0.000 description 4
- 238000009499 grossing Methods 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 229920001577 copolymer Polymers 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000002304 perfume Substances 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- 229940043375 1,5-pentanediol Drugs 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- XMSXQFUHVRWGNA-UHFFFAOYSA-N Decamethylcyclopentasiloxane Chemical compound C[Si]1(C)O[Si](C)(C)O[Si](C)(C)O[Si](C)(C)O[Si](C)(C)O1 XMSXQFUHVRWGNA-UHFFFAOYSA-N 0.000 description 2
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 229930182558 Sterol Natural products 0.000 description 2
- 230000037374 absorbed through the skin Effects 0.000 description 2
- 230000003570 biosynthesizing effect Effects 0.000 description 2
- 229940094978 bis-peg-18 methyl ether dimethyl silane Drugs 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940093499 ethyl acetate Drugs 0.000 description 2
- 235000019439 ethyl acetate Nutrition 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- -1 pack Substances 0.000 description 2
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 description 2
- 238000005191 phase separation Methods 0.000 description 2
- 150000003432 sterols Chemical class 0.000 description 2
- 235000003702 sterols Nutrition 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- CUNWUEBNSZSNRX-RKGWDQTMSA-N (2r,3r,4r,5s)-hexane-1,2,3,4,5,6-hexol;(z)-octadec-9-enoic acid Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CCCCCCCC\C=C/CCCCCCCC(O)=O.CCCCCCCC\C=C/CCCCCCCC(O)=O.CCCCCCCC\C=C/CCCCCCCC(O)=O CUNWUEBNSZSNRX-RKGWDQTMSA-N 0.000 description 1
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 1
- RYCNUMLMNKHWPZ-SNVBAGLBSA-N 1-acetyl-sn-glycero-3-phosphocholine Chemical compound CC(=O)OC[C@@H](O)COP([O-])(=O)OCC[N+](C)(C)C RYCNUMLMNKHWPZ-SNVBAGLBSA-N 0.000 description 1
- OMIGHNLMNHATMP-UHFFFAOYSA-N 2-hydroxyethyl prop-2-enoate Chemical compound OCCOC(=O)C=C OMIGHNLMNHATMP-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001107116 Castanospermum australe Species 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 240000004270 Colocasia esculenta var. antiquorum Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 206010012438 Dermatitis atopic Diseases 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241001079007 Phellodendron chinense Species 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000021279 black bean Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229940082500 cetostearyl alcohol Drugs 0.000 description 1
- 229940085262 cetyl dimethicone Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229940086555 cyclomethicone Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000010438 granite Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229940057874 phenyl trimethicone Drugs 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 description 1
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 description 1
- 229940113124 polysorbate 60 Drugs 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 150000003248 quinolines Chemical class 0.000 description 1
- 235000020095 red wine Nutrition 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000036555 skin type Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229960005078 sorbitan sesquioleate Drugs 0.000 description 1
- 229940031439 squalene Drugs 0.000 description 1
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 125000000383 tetramethylene group Chemical group [H]C([H])([*:1])C([H])([H])C([H])([H])C([H])([H])[*:2] 0.000 description 1
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- LINXHFKHZLOLEI-UHFFFAOYSA-N trimethyl-[phenyl-bis(trimethylsilyloxy)silyl]oxysilane Chemical compound C[Si](C)(C)O[Si](O[Si](C)(C)C)(O[Si](C)(C)C)C1=CC=CC=C1 LINXHFKHZLOLEI-UHFFFAOYSA-N 0.000 description 1
- GPPXJZIENCGNKB-UHFFFAOYSA-N vanadium Chemical compound [V]#[V] GPPXJZIENCGNKB-UHFFFAOYSA-N 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/11—Encapsulated compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/965—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of inanimate origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
Definitions
- the present invention relates to a method for extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract encapsulated by the nanoemulsion technology to improve transdermal permeation, in which the extracts contain various minerals or active ingredients. Also, the present invention relates to a cosmetic composition comprising glacier water as an active ingredient to supply moisture to the skin, to reduce loose keratin and to soften the skin texture.
- the glacier water is formed in the polar regions, one of the cleanest regions on the earth or high mountains, representing health and pure liveliness of the nature.
- Representative glacier water-producing regions include Vilcabamba of Ecuador, Abkhasia of Caucasus or Hunza of Himalaya.
- the glacier water has a structure in which microorganisms or pathogenic bacteria hardly propagate and has a high rate of hexagonal water. Also, it contains a large amount of minerals. Therefore, the regions where the glacier water is produced are famous for long-lived towns.
- This conventional solvent extraction method has several problems such as safety related problems due to residual organic solvents and formulability and stability related problems upon combination of cosmetic products and thus, there is a demand for a novel solvent extraction method to solve those problems.
- the present inventors have conducted studies to solve the skin safety related problems caused the residual organic solvents the prior and to find a solvent substituting for the organic solvents.
- the present inventors have found that when active ingredients are extracted using glacier water instead of water or organic solvents, it is possible to increase absorption of the active ingredients while maintaining safety to the skin.
- by encapsulating the extract prepared therefrom in nanoemulsion particles by nano technology it is possible to provide a cosmetic composition with improvement of transdermal absorption.
- the present invention has been completed.
- the present inventors have found that the molecular structure of glacier water is similar to that of water molecule found in the living tissue and thus, readily absorbed through the skin, leading excellent moisturizing effect. On the basis of this founding, the present invention has been completed.
- the present invention provides a method of extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract encapsulated by the nanoemulsion technology, as an active ingredient, to improve transdermal permeation, in which the extract contains various minerals or effective ingredients.
- the present invention provides a cosmetic composition
- a cosmetic composition comprising glacier water as an active ingredient.
- the extraction of active ingredients is performed by using glacier water rich in natural minerals.
- glacier water rich in natural minerals it is possible to provide safety upon application to the skin, increase effects of active ingredients.
- transdermal absorption of a cosmetic composition is maximized by stabilizing the extract in nanoemulsion particles through encapsulation using an emulsion affinitive to skin.
- FIG. 2 shows the amount of keratin after using Comparative Formulation 4.
- FIG. 4 shows the skin roughness after using Comparative Formulation 4.
- FIG. 6 shows the skin roughness after using Comparative Formulation 5.
- FIG. 9 is a photograph showing the roughness of the stratum corneum after using Formulation 3.
- the skin lotions of Formulation 1 and Comparative Formulations 1 to 3 were prepared using the ingredients described in Table 2 below according to a conventional method (unit: wt%).
- the stratum corneum samples were taken from both the group who applied the skin toner (Formulation 2) or cream (Formulation 3) containing glacier water (supplied from Huza of Himalaya) to the skin and the group who applied the skin toner (Comparative Formulation 4) or cream (Comparative Formulation 5) without containing glacier water to the skin and subjected to a pretreatment. Then, the samples were measured for the roughness and the results were compared. Each group includes 20 healthy adult women.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a method for extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract encapsulated by the nanoemulsion technology to improve transdermal permeation, in which the extracts contain various minerals or active ingredients. Also, the present invention relates to a cosmetic composition comprising glacier water as an active ingredient to supply moisture to the skin, to reduce loose keratin and to soften the skin texture.
Description
The present invention relates to a method for extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract encapsulated by the nanoemulsion technology to improve transdermal permeation, in which the extracts contain various minerals or active ingredients. Also, the present invention relates to a cosmetic composition comprising glacier water as an active ingredient to supply moisture to the skin, to reduce loose keratin and to soften the skin texture.
In high mountains or polar regions, snow has been accumulated without thawing for a long period of time to form perpetual snow, which turns hard icecaps by pressure. Here, water produced from the icecaps is called "glacier water".
At present, the glacier water is formed in the polar regions, one of the cleanest regions on the earth or high mountains, representing health and pure liveliness of the nature. Representative glacier water-producing regions include Vilcabamba of Ecuador, Abkhasia of Caucasus or Hunza of Himalaya.
The glacier water has a structure in which microorganisms or pathogenic bacteria hardly propagate and has a high rate of hexagonal water. Also, it contains a large amount of minerals. Therefore, the regions where the glacier water is produced are famous for long-lived towns.
Minerals support osmotic pressure and acid-alkali equilibrium in the living body, control healing of the wounded and metabolic functions in the body and play an important role in oxygen transmission and cell respiration. Therefore, studies on a novel solvent extraction method to increase transdermal absorption of the minerals are actively in progress.
According to the conventional solvent extraction method, an extraction process including mixing an object material with water; an organic solvent such as ethanol, methanol, butanol, ether, ethylacetate and chloroform; or a combination of water and an organic solvent and leaving the mixture at room temperature for one day, followed by extraction, is repeated at least twice to obtain an extract. The extract is filtered and the resulting filtrate is concentrated in a vacuum concentrator to obtain a primary product.
To the primary product, water and an organic solvent such as ethanol, methanol, butanol, ether, ethylacetate or chloroform are added and left at room temperature for 2 hours for phase separation. The aqueous phase was removed and the organic solvent is further added. This procedure is repeated at least 2 times for sufficient washing, followed by filtering. The resulting filtrate is dried in a vacuum oven to obtain the desired extract.
This conventional solvent extraction method has several problems such as safety related problems due to residual organic solvents and formulability and stability related problems upon combination of cosmetic products and thus, there is a demand for a novel solvent extraction method to solve those problems.
Also, it is greatly desired to develop a novel solvent extraction method to increase absorption of active ingredients such as minerals while securing safety.
Accordingly, the present inventors have conducted studies to solve the skin safety related problems caused the residual organic solvents the prior and to find a solvent substituting for the organic solvents. As a result, the present inventors have found that when active ingredients are extracted using glacier water instead of water or organic solvents, it is possible to increase absorption of the active ingredients while maintaining safety to the skin. Also, by encapsulating the extract prepared therefrom in nanoemulsion particles by nano technology, it is possible to provide a cosmetic composition with improvement of transdermal absorption. On the basis of this development, the present invention has been completed.
Further, the present inventors have found that the molecular structure of glacier water is similar to that of water molecule found in the living tissue and thus, readily absorbed through the skin, leading excellent moisturizing effect. On the basis of this founding, the present invention has been completed.
Therefore, it is an object of the present invention to provide a method of extracting an active ingredient for safe application to skin and excellent absorption of the active ingredient and an extract prepared therefrom, and a cosmetic composition comprising the extract as an active ingredient.
Also, it is an object of the present invention to provide a cosmetic composition for increasing skin moisturizing effect, reducing keratin formation and smoothing skin texture.
To achieve the above objects, the present invention provides a method of extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract encapsulated by the nanoemulsion technology, as an active ingredient, to improve transdermal permeation, in which the extract contains various minerals or effective ingredients.
Further, the present invention provides a cosmetic composition comprising glacier water as an active ingredient.
According to the present invention, the extraction of active ingredients is performed by using glacier water rich in natural minerals. As a result, it is possible to provide safety upon application to the skin, increase effects of active ingredients. Also, transdermal absorption of a cosmetic composition is maximized by stabilizing the extract in nanoemulsion particles through encapsulation using an emulsion affinitive to skin.
In addition, the cosmetic composition comprising the glacier water according to the present invention has superior skin moisturizing effect and increase skin moisture content, thereby reducing keratin and roughness and smoothing the skin surface.
The above and other objects, features and advantages of the present invention will be apparent from the following detailed description of the preferred embodiments of the invention in conjunction with the accompanying drawings, in which:
FIG. 1 shows the amount of keratin after using Formulation 2.
FIG. 2 shows the amount of keratin after using Comparative Formulation 4.
FIG. 3 shows the skin roughness after using Formulation 2.
FIG. 4 shows the skin roughness after using Comparative Formulation 4.
FIG. 5 shows the skin roughness after using Formulation 3.
FIG. 6 shows the skin roughness after using Comparative Formulation 5.
FIG. 7 is a photograph showing the roughness of the stratum corneum after using Formulation 2.
FIG. 8 is a photograph showing the roughness of the stratum corneum after using Comparative Formulation 4.
FIG. 9 is a photograph showing the roughness of the stratum corneum after using Formulation 3.
FIG. 10 is a photograph showing the roughness of the stratum corneum after using Comparative Formulation 5.
Reference will now be made in detail to the preferred embodiment of the present invention with reference to the attached drawings.
Hereinafter, the present invention will be described in detail.
The glacier water used according to the present invention may include any one produced in Hunza of Himalaya, Vilcabamba of Ecuador or Abkhasia of Caucasus, preferably Himalaya glacier water produced in Hunza of Himalaya.
The Himalaya glacier water, also called "Hunza water" has a unique colloidal structure of nano-sized silica particles. Accordingly, it has a low surface tension and thereby, can let an active ingredient be readily absorbed to the human body or skin. Also, the Himalaya glacier water has a molecular structure of a bird cage shape similar to a hemisphere dome, which can trap a large amount of hydrogen. Such water molecule structure has a small amount of colloidal inorganic compound particles having a high zeta potential. Here, the shape of the formed water molecule is known to be considerably similar to that of the water molecule found in the living tissue but quite different from that of the water molecule found in common mineral waters and tap water.
According to the present invention, there is provided a method of solvention extraction using glacier water. The method comprises the steps of:
1) repeating an extraction process at least two times and concentrating the resulting filtrate in a vacuum concentrator to obtain a primary product, in which the extraction process includes immersing a plant in glacier water at room temperature for one day and filtering; and
2) adding glacier water to the product, stirring the mixture at room temperature for at least 2 hours, followed by sufficient washing, filtering the mixture and drying in a vacuum oven to obtain an extract.
In the present invention, the glacier water has undergone natural filtering apparatuses such as granite, gravel and small grains, while it thawed into water. The filtered glacier water is obtained from the ground water aquifer at the depth of several kilometers under the ground and is clean and not contaminated since while protected by a natural barrier of granitic layer. Therefore, the method of the solvent extraction using the glacier water according to the present invention simplifies the filtration process unlike the conventional methods using organic solvents, thereby readily producing the extract.
The effective minerals such as sodium, magnesium, potassium and calcium, and zinc, copper, vanadium and manganese contained the glacier water can be rapidly absorbed through the skin. Also, the glacier water has purifying effect by removing toxic substances and thus, microorganisms or bacteria cannot propagate therein. Therefore, according to the present invention, there is provided a cosmetic composition which can be used with regardless to skin types of users and be even safely used by infants or those with skin troubles such as atopic dermatitis patients.
The active ingredients which can be extracted by the method according to the present invention includes, but are not particularly limited, β-1,3-glucan, papain, polyphenol, saponin, adenosine, vitamin C (ascorbic acid), arbutin, niacinamide and acetylglucosamine.
The β-1,3-glucan extracted by the method according to the present invention is a glucan having a high purity of at least 98% and extracted from mycelium Schizophyllum commune and effective in prevention of skin aging and healing of damaged skin. Also, in addition to Schizophyllum commune, the β-1,3-glucan can be extracted from Ganoderma japonicum, Agaricus blazei Muill, Lentinus edodes, Sclerotinia sclerotiorum, yeast and barley using the glacier water according to the solvent extraction method according to the present invention.
Further, the papain of the active ingredients can be extracted from fruit or tree of papaya and polyphenol can be extracted from anthocyanin pigments of red or purple colors contained in green tea, coffee, strawberries, eggplant, red beans and the like, red wine, black bean or cacao.
Saponin can be extracted from bean, ginseng or yam, adenosine can be extracted from plants such as wild parsley, vitamin C (ascorbic acid) can be extracted from Rubus Coreanus, persimmon tree leaf, rosehip, grapefruit or pimento, arbutin can be extracted from Arctostaphylos uva ursi, niacinamide can be extracted from potato or avocado and acetylglucosamine can be extracted from the dried bark of 
and Phellodendron chinense SCHNEID.
Further, according to the present invention, the extract (active ingredients) extracted using glacier water can be encapsulated into fine emulsion particles or liposomes by applying nanoemulsion technology, whereby, the transdermal absorption of minerals contained in the glacier water is increased. The extracted with the glacier water is contained in the amount of 0.001 to 50 wt% based on the total weight of the emulsion particles or liposomes. When the amount of the extract is less than 0.001 wt%, the effect of the active ingredients is not sufficiently expressed. When the amount exceeds 50 wt%, the formulation is hard. According to the present invention, the nanoemulsion particles comprising the extract can be prepared by a conventional high pressure emulsifying method, as a nanoemulsion technology, at a pressure of 500 to 1000 bar.
The emulsion particles according to the present invention have a diameter of 1000 nm to 30 nm, preferably 300 nm to 50 nm. The nanoemulsion particles or liposomes have the contact surface with the skin increased and thus, the area for transdermal absorption increased. Also, the emulsion particles according to the present invention can be readily absorbed or diffused into lipid between cells since the gap between lipid molecules in the stratum corneum is about 50 nm and the emulsion membrane of the emulsion particles is flexible. That is, the emulsion particles having an average diameter of 300 nm to 50 nm, as prepared by the nanoemulsion technology, increase the transdermal permeation of the emulsion particles themselves and the extract in the emulsion particle by increase in contact area with the skin; permeation and diffusion through lipids between cells; and the extract extracted by using glacier water.
Meanwhile, the emulsion particles according to the present invention may further comprise lecithin as an emulsifying agent along with the extract and the content of lecithin is 0.5 to 5 wt%, preferably 2 to 4 wt%, based on the total weight of the emulsion particles. The content of lecithin is an effective amount to form a stable formulation of the cosmetic composition. The lecithin comprises unsaturated choline based compounds such as phosphatidylcholine, lysophosphatidylcholine and phosphatidylethanolamine, and hydrogenates thereof.
Also, according to the present invention, the emulsion particles may include various non-ionic surfactants as a subsidiary emulsifying agent, preferably fatty alcohol complex surfactants or high molecular weight surfactants. The subsidiary emulsifying agent is used in a weight ratio of 0.1 to 5 times, preferably 0.5 to 2 times, based on the weight of lecithin according to the weight and ingredients of lecithin.
Also, according to the present invention, there is provide a cosmetic composition comprising emulsion particles or liposomes having the extract, extracted by using glacier water, encapsulated therein. The cosmetic composition according to the present invention contains the emulsion particles or liposomes in the amount of 0.001 to 50 wt% based on the total weight of the composition. When the content of the emulsion particles or liposomes is less than 0.001 wt%, the active ingredients hardly express their effects in formulations. When the content exceeds 50 wt%, the formulation is hard. Further, the cosmetic composition may selectively further comprises glacier water as an active ingredient.
In addition, according to the present invention, there is provided a cosmetic composition comprising glacier water. The glacier water is contained in the amount of 0.1 to 100 wt% based on the total weight of the composition. When the content of the glacier water is less than 0.1 wt%, the effect of glacier water is poor. The cosmetic composition comprising glacier water according to the present invention can supply moisture to the skin, reduce keratin in the skin and reducing the skin roughness, thereby softening the skin texture.
The cosmetic composition according to the present invention can be formulated in various formulations without any particular limitation. For example, the formulations include skin softener, skin toner, skin lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, body essence, make-up base, foundation, hair manicure, shampoo, rinse, body cleanser, toothpaste and mouth gaggle, skin whitening cosmetic products or medical supplies such as ointment and patch.
Now, the present invention is described in detail by the following examples, but the scope of the present invention is not limited thereto.
Example 1: Preparation of β-1,3-glucan extract using glacier water
1 kg of dried mycelium of Schizopyllum commune was added to 4 L of glacier water of Himalaya and stored at room temperature for one day, followed by extraction. This procedure was repeated at least twice to obtain an extract, which was then filtered. The filtrate was concentrated in a vacuum concentrator to obtain 400 g of a primary product. 400 g of the product was added to 1 L of glacier water, stirred at room temperature for at least 2 hours, thoroughly washed and filtered. The filtered product was dried in a vacuum oven to obtain about 30 g of the β-1,3-glucan extract containing 70% or more of Schizophyllum commune.
Comparative Example 1
1 kg of dried mycelium of Schizopyllum commune was added to 4 L of ethanol and stored at room temperature for one day, followed by extraction. This procedure was repeated at least twice to obtain an extract, which was then filtered. The filtrate was concentrated in a vacuum concentrator to obtain 400 g of a primary product. To the resulting product, 1 L of water and 1 L of ethanol were added, stirred at room temperature for at least 2 hours and left for phase separation. The water phase was removed and 1 L of ethanol was further added. This procedure was repeated at least twice, thoroughly washed and filtered. The filtered product was dried in a vacuum oven to obtain the β-1,3-glucan extract.
Example 2: Preparation of nanoemulsion particles
4 g of hydrogenated lecithin, 4 g of PEG-5 rapeseed sterol, 5 g of polyethyleneglycol and 5 g of pentyleneglycol were mixed together and added to a solution of 1 g of β-1,3-glucan extract prepared in Example 1 dissolved in 10 g of ethanol, which was then was heated to 70 to 75℃ for complete dissolution. The resulting solution was mixed with the water part (65.95 g of distilled water, 5 g of glycerin and 0.05 g of EDTA), which had been previously heated, pre-emulsified using a common homo mixer (3 minutes, 3,000 to 6,000 rpm) and emulsified using a high pressure microfluidizer at 800 Bar/3 cycles.
Comparative Example 2
4 g of hydrogenated lecithin, 4 g of PEG-5 rapeseed sterol, 5 g of polyethyleneglycol, 5 g of pentyleneglycol and 10 g of ethanol were mixed together and heated to 70 to 75℃ for complete dissolution. The resulting solution was mixed with the water part (66.95 g of distilled water, 5 g of glycerin and 0.05 g of EDTA), which had been previously heated, pre-emulsified using a common homo mixer (3 minutes, 3,000 to 6,000 rpm) and emulsified using a high pressure microfluidizer at 800 Bar/3 cycles.
Comparative Example 3
Except for substituting the β-1,3-glucan extract prepared in Comparative Example 1 with the β-1,3-glucan extract prepared in Example 1, the same procedure as above Example 2 was performed to obtain nanoemulsion particles.
Experimental Example 1: Effect of biosynthesizing collagen in human dermal cells
Human fibroblasts were cultured in 24 well plates. The medium was exchanged with the medium containing the compositions of the concentrations described in Table 1. The cells were cultured for further 3 days. Each well was treated with 0.5 ml of DMEM containing 10% fetal bovine serum and then, L[2, 3, 4, 5-3H]-proline 10 μCi. After 24 hours, the medium and the cells were collected from each well, washed with 5% trichloroacetic acid (TCA) solution and distributed to 2 test tubes. 1 unit/㎕ of type I collagenase was added to one test tube and incubated at 37℃ for 90 minutes. The other test tube was stored at 4℃. Next, the test tubes were treated with 0.05 ml of 50% trichloroacetic acid, stored at 4℃ for 20 minutes, centrifuged at 12,000 rpm for 10 minutes. The supernatant and the precipitation were separately measured for CPM (counts per minute) using a liquid scintillation counter (LSC). By the following Math Figure 1, values of relative collagen biosynthesis (RCB) of the control and the treatment groups. The result is shown in Table 1 below.
MathFigure 1 
Table 1 
From the result of Table 1, it was noted that the nanoemulsion prepared using the β-1,3-glucan extract extracted with Himalaya glacier water increased collagen biosynthesis concentration-dependently.
Formulation 1 and Comparative Formulations 1 to 3: Preparation of skin lotion
The skin lotions of Formulation 1 and Comparative Formulations 1 to 3 were prepared using the ingredients described in Table 2 below according to a conventional method (unit: wt%).
Table 2
| Ingredients | Formulation 1 | Comparative Formulation 1 | Comparative Formulation 2 | |
| Nanoemulsion particles prepared in Example 2 | 10.0 | - | - | - |
| Nanoemulsion particles prepared in Comparative Example 2 | - | 10.0 | - | - |
| Nanoemulsion particles prepared in Comparative Example 3 | - | - | 10.0 | - |
| Cetylethylhexanoate | 5.0 | 5.0 | 5.0 | 5.0 |
| Cetostearylalcohol | 1.0 | 1.0 | 1.0 | 1.0 |
| Liphophilic monosteric stearate | 0.8 | 0.8 | 0.8 | 0.8 |
| Squalene | 2.0 | 2.0 | 2.0 | 2.0 |
| Polysorbate 60 | 1.5 | 1.5 | 1.5 | 1.5 |
| Sorbitan Sesquioleate | 0.5 | 0.5 | 0.5 | 0.5 |
| Polyethyleneglycol | 5.0 | 5.0 | 5.0 | 5.0 |
| Triethanolamine | 0.2 | 0.2 | 0.2 | 0.2 |
| Carboxyvinyl polymer | 0.2 | 0.2 | 0.2 | 0.2 |
| Preservative | q.s. | q.s. | q.s. | q.s. |
| Colorant | q.s. | q.s. | q.s. | q.s. |
| Perfume | q.s. | q.s. | q.s. | q.s. |
| Purified water | To 100 | To 100 | To 100 | To 100 |
Experimental Example 2: Effect of biosynthesizing collagen in animal
In order to confirm the collagen biosynthesis increasing effect of β-1,3-glucan extracted with glacier water according to the present invention, the skin lotions prepared in Formulation 1 and Comparative Formulations 1 to 3 were applied to the back of hairless mice for a week. Then, the back skin was biopsed and subjected to the immumohistochemistry for collagen. The data were calculated relative to 100 of Comparative Formulation 3, which had not been treated. The result is shown in Table 3.
Table 3
| Test material | Collagen biosynthesis (%) |
| Formulation 1 | 135 |
| Comparative Formulation 1 | 105 |
| Comparative Formulation 2 | 128 |
| | 100 |
From the result of Table 3, the collagen biosynthesis was increased in the group applied with Formulation 1 by 28.6% and 5.5%, as compared to Comparative Formulations 1 and 2, respectively. Accordingly, it was confirmed that the β-1,3-glucan extract extracted with Himalaya glacier water according to the present invention increased the collagen biosynthesis increasing effect by increasing the transdermal absorption.
Experimental Example 3: Measurement of skin elasticity improving effect in human skin
The skin lotions prepared according to Table 2 above were examined for the skin elasticity improving effect. 20 healthy women, at least 30 years old, were divided into two group and stood by at a temperature of 24 to 26℃ and a humidity of 75%. Each group of women was applied on the face with the skin toner of Formulation 1 or Comparative Formulation 2 twice a day for 12 weeks. Then, the skin elasticity was measured using the cutometer SEM 575 (C+K Electronic Co., Germany). The data were values of R8 [R8(left)-R8(right)] of the Cutometer SEM 575 and the result is shown in Table 4 below. The R8 value represented viscoelasticity of skin.
Table 4
| Test material | Skin elasticity |
| Formulation 1 | 0.33 |
| Comparative Formulation 2 | 0.10 |
From the result of Table 4, it was noted that the skin elasticity was increased more in the group applied with Formulation 1 containing the β-1,3-glucan extract extracted with Himalaya glacier water according to the present invention, as compared to Comparative Formulations 2.
Separately, after the experiment, the participants were asked to respond to a questionnaire for the subjective evaluation in addition to the mechanical evaluation. The result is shown in Table 5 below.
Table 5
| Test material | Respondents | |||
| Very good | good | Fair | Poor | |
| Formulation 1 | 3 | 6 | 1 | 0 |
| Comparative Formulation 2 | 1 | 3 | 3 | 3 |
Also, from the questionnaire, it was confirmed that Formulation 1 containing the β-1,3-glucan extract extracted with Himalaya glacier water according to the present invention improved the skin elasticity.
Formulation 2 and Comparative Formulation 4: Preparation of skin toner
The skin toners of Formulation 2 and Comparative Formulation 4 were prepared using the ingredients described in Table 6 below according to a conventional method (unit: wt%).
Table 6
| Ingredients | Formulation 2 | Comparative Formulation 4 |
| Glacier water(Hunza gracia water) | 3.00 | - |
| β-1,3-glucan extract from Example 1 | 1.10 | - |
| β-1,3-glucan extract from Comparative Example 1 | - | 1.10 |
| Disodium EDTA | 0.02 | 0.02 |
| DL-pantenol | 0.05 | 0.05 |
| Butylene glycol | 2.00 | 2.00 |
| Glycereth-26 | 6.00 | 6.00 |
| PEG.PPG-17.6 copolymer | 2.00 | 2.00 |
| Hydroxyethylacrylate.sodiumacryloyldimethyltaurate copolymer | 0.65 | 0.65 |
| Ethanol | 5.00 | 5.00 |
| Bis-PEG-18 methyl ether dimethyl silane (Dimethicone Copolyol) | 0.20 | 0.20 |
| PolyoxyethyleneHydrogenated caster oil | 0.30 | 0.30 |
| Phenyltrimethicone | 0.10 | 0.10 |
| Preservative | q.s. | q.s. |
| Perfume | q.s. | q.s. |
| Purified water | To 100 | To 100 |
The creams of Formulation 3 and Comparative Formulation 5 were prepared using the ingredients described in Table 7 below according to a conventional method (unit: wt%).
Table 7
| | Formulation | 3 | |
| glacier water (Hunza gracia water) | 1.00 | - | |
| β-1,3-glucan from Example 1 | 1.10 | - | |
| β-1,3-glucan from Comparative Example 1 | - | 1.10 | |
| Disodium EDTA | 0.02 | 0.02 | |
| PEG-75 | 1.00 | 1.00 | |
| Bis-PEG-18 methyl ether dimethyl silane (Dimethicone Copolyol) | 0.5 | 0.5 | |
| Butylenes glycol | 5.00 | 5.00 | |
| Glycerin (conc. Glycerin) | 2.00 | 2.00 | |
| Cetyl PEG.PPG-10.1dimethicone (cetyl dimethicone Copolyol) | 0.500 | 0.500 | |
| PEG-7 dimethicone (Dimethicone Copolyol, polyoxyethylene methylpolysiloxane copolymer) | 2.50 | 2.50 | |
| Dimethicone | 3.00 | 3.00 | |
| Decamethylcyclopentasiloxane (cyclomethicone) | 4.00 | 4.00 | |
| Butyleneglycol dicaprilate.dicaprate | 3.00 | 3.00 | |
| Preservative | q.s. | q.s. | |
| Perfume | q.s. | q.s. | |
| Purified water | To 100 | To 100 |
Experimental Example 4: Measurement of increase in moisture content in skin through moisture supply by using glacier water
The group who applied the skin toner (Formulation 2) or cream (Formulation 3) containing glacier water (supplied from Huza of Himalaya) to the skin and the group who applied the skin toner (Comparative Formulation 4) or cream (Comparative Formulation 5) without containing glacier water were measured for the moisture content in the skin and their results were compared. The skin toner and the cream were separately applied different groups to examine the increase in the moisture content by each product without interaction between the products. Each group includes 20 healthy adult women.
In the first visit, the forearm was washed clean and stood by for 30 minutes in a constant-temperature and constant-humidity room (23℃, RH 40%) for adaptation of the skin. After 30 minutes later, the initial moisture content of the skin in the forearm was measured using a corneometer (CM825, C+K Electronic Co., Germany). The corneometer measures the moisture content in the skin by measuring electrical conductivity of the skin. Then, 2 ㎕/㎠ of the cosmetic composition was applied to 15 ㎠ of the previously indicated part of the forearm. After 3 hours, 6 hours and 9 hours (including 12 hours for the skin toner), the part of the skin where each Formulation was applied was measured for the water content. The result of the group using the product containing glacier water was compared with that of the group using the product without containing glacier water to determine the moisturization and water-retaining power of the products. The result is shown in Table 8 (unit: AU).
Table 8
| | 0 | 3 hours | 6 | 9 hours | 12 hours |
| Formulation 2(skin toner AD, with containing glacier water ) | 30 | 51 | 43 | 38 | 35 |
| Comparative Formulation 4(skin toner, without containing glacier water) | 31 | 46 | 38 | 35 | 33 |
| Formulation 3(cream AD, with containing glacier water) | 26 | 68 | 55 | 51 | - |
| Comparative Formulation 5(cream, without containing glacier water) | 26 | 58 | 50 | 46 | - |
From the result of Table 8, it was noted that the skin applied with Formulations 2 and 3 contained more moisture than the skin applied with Comparative Formulations 4 and 5.
Experimental Example 5: Measurement of reduction in amount of keratin through moisture supply by using glacier water
Since the amount of keratin was reduced, the group who applied the skin toner (Formulation 2) or cream (Formulation 3) containing glacier water (supplied from Huza of Himalaya) to the skin and the group who applied the skin toner (Comparative Formulation 4) or cream (Comparative Formulation 5) without containing glacier water were measured for the amount of keratin in the skin by image analysis and their results were compared. Each group includes 20 healthy adult women.
In the first visit, the forearm was washed clean and stood by for 30 minutes in a constant-temperature and constant-humidity room (23℃, RH 40%) for adaptation of the skin. After 30 minutes later, in order to measure the initial amount of keratin of the forearm, loose keratin was taken using a tape. Then, 2 ㎕/㎠ of the cosmetic composition was applied to 15 ㎠ of the previously indicated part of the forearm. After 3 hours, 6 hours and 9 hours (including 12 hours for the skin toner), the part of the skin where each Formulation was applied was measured for the keratin amount. The result of the group using the product containing glacier water was compared with that of the group using the product without containing glacier water by image analysis and it was confirmed that the product containing glacier water had the skin moisturizing and soothing effect. The result is shown in Table 9 and FIG. 1 and FIG. 2 (unit: AU).
Table 9
| | 0 | 3 hours | 6 | 9 hours | 12 hours |
| Formulation 2(skin toner AD, with containing glacier water ) | 24 | 10 | 10 | 12 | 15 |
| Comparative Formulation 4(skin toner, without containing glacier water) | 24 | 15 | 13 | 15 | 16 |
| Formulation 3(cream AD, with containing glacier water) | 19 | 2 | 4 | 5 | - |
| Comparative Formulation 5(cream, without containing glacier water) | 19 | 4 | 5 | 6 | - |
From the results of Table 9 above and FIG. 1 and FIG. 2, it was noted that Formulations 2 and 3 reduced the keratin amount much more than Comparative Formulations 4 and 5 and thus, the products containing glacier water were more effective in moisturizing and soothing the skin.
Experimental Example 6: Measurement of reduction in skin roughness through moisture supply by using glacier water
The group who applied the skin toner (Formulation 2) or cream (Formulation 3) containing glacier water (supplied from Huza of Himalaya) to the skin and the group who applied the skin toner (Comparative Formulation 4) or cream (Comparative Formulation 5) without containing glacier water were examined for the skin roughness and their results were compared. Each group includes 20 healthy adult women.
In the first visit, the forearm was washed clean and stood by for 30 minutes in a constant-temperature and constant-humidity room (23℃, RH 40%) for adaptation of the skin. After 30 minutes later, the skin roughness was measured using a 3D scanner, PRMOS compact (G.F.M., Germany). Then, 2㎕/㎠ of the cosmetic composition was applied to 15㎠ of the previously indicated part of the forearm. After 9 hours (including 12 hours for the skin toner), the same part of the skin was measured for the skin roughness. The result of the group using the product containing glacier water was compared with that of the group using the product without containing glacier water and it was confirmed that the product containing glacier water had the effect of smoothing skin texture. The result is shown in FIG. 3 to FIG. 6.
From the results of FIG. 3 to FIG. 6, it was noted that Formulation 2 and Formulation 3 reduced the skin roughness much more than Comparative Formulation 4 and Comparative Formulation 5 and thus, the products containing glacier water were more effective in smoothing the skin texture.
Experimental Example 7: Measurement of reduction in roughness of the stratum corneum by using glacier water
The stratum corneum samples were taken from both the group who applied the skin toner (Formulation 2) or cream (Formulation 3) containing glacier water (supplied from Huza of Himalaya) to the skin and the group who applied the skin toner (Comparative Formulation 4) or cream (Comparative Formulation 5) without containing glacier water to the skin and subjected to a pretreatment. Then, the samples were measured for the roughness and the results were compared. Each group includes 20 healthy adult women.
In the first visit, the forearm was washed clean and stood by for 30 minutes in a constant-temperature and constant-humidity room (23℃, RH 40%) for adaptation of the skin. After 30 minutes later, samples of the stratum corneum were taken (Skin surface biopsy). Then, 2 ㎕/㎠ of the cosmetic composition was applied to 15 ㎠ of the previously indicated part of the forearm. After 9 hours (9 hours and 12 hours for the skin toner), samples of the stratum corneum in the same area were taken, subjected to a pretreatment and examined for the roughness of the stratum corneum by an electron microscope. The result of the group using the product containing glacier water was compared with that of the group using the product without containing glacier water and it was confirmed that the product containing glacier water had the effect of soothing the skin. The result is shown in FIG. 7 to FIG. 10.
From the results of FIG. 7 to FIG. 10, it was noted that Formulations 2 and 3 reduced the roughness of the stratum corneum much more than Comparative Formulations 4 and 5 and thus, the products containing glacier water were more effective in soothing the skin.
While the present invention has been described with reference to the particular illustrative embodiments, it is not to be restricted by the embodiments but only by the appended claims. It is to be appreciated that those skilled in the art can change or modify the embodiments without departing from the scope and spirit of the present invention.
Claims (20)
- A method of extracting an active ingredient from plants by using glacier water as an extraction solvent.
- The method of Claim 1, which comprises the steps of:1) repeating an extraction process at least two times and concentrating the resulting filtrate in a vacuum concentrator to obtain a primary product, in which the extraction process includes immersing a plant in glacier water at room temperature for one day and filtering; and2) adding glacier water to the product, stirring the mixture at room temperature for at least 2 hours, followed by sufficient washing, filtering the mixture and drying in a vacuum oven to obtain an extract.
- The method of claim 1, wherein the glacier water is produced in Hunza of Himalaya, Vilcabamba of Ecuador or Abkhasia of Caucasus.
- The method of Claim 1, wherein the plant is at least one selected from the group consisting of Schizophyllum commune, Ganoderma japonicum, Agaricus blazei Muill, Lentinus edodes, Sclerotinia sclerotiorum, yeast, barley, papaya, green tea, coffee, strawberry, eggplant, grapes, red bean, black soybean, cacao, beans, ginseng, yam, wild parsley, Rubus Coreanus, persimmon tree leaf, rosehip, grapefruit, pimento, Arctostaphylos uva ursi, potato, avocado and Phellodendron amurense Ruprecht.
- The method of Claim 1, wherein the active ingredient is at least one selected from the group consisting of β-1,3-glucan, papain, polyphenol, saponin, adenosine, vitamin C (ascorbic acid), arbutin, niacinamide and acetylglucosamine.
- A nanoemulsion particle comprising an active ingredient extracted according to the method of Claim 1 and lecithin and having a diameter of 1000 to 30 nm.
- The nanoemulsion particle of Claim 6, wherein the active ingredient is contained in the amount of 0.001 to 50 wt% based on the total weight of the nanoemulsion particle.
- The nanoemulsion particle of Claim 6, wherein the lecithin is contained in the amount of 0.5 to 5 wt% based on the total weight of the nanoemulsion particle.
- A cosmetic composition comprising the nanoemulsion particle according to any one of Claims 6 to 8 as an active ingredient.
- The composition of Claim 9, which further comprises glacier water as an active ingredient.
- A cosmetic composition comprising glacier water as an active ingredient.
- The composition of Claim 11, wherein the glacier water is contained in the amount of 0.1 to 100 wt% based on the total weight of the composition.
- The composition of Claim 11, wherein the glacier water is produced in Hunza of Himalaya, Vilcabamba of Ecuador or Abkhasia of Caucasus.
- The composition of Claim 11, which is to moisturize the skin.
- The composition of Claim 11, which is to reduce keratin.
- The composition of Claim 11, which is to relieve roughness of the skin surface.
- A use of glacier water for extracting an active ingredient from plants as an extraction solvent.
- A use of glacier water for moisturizing skin in a cosmetic composition.
- A use of glacier water for reducing keratin in a cosmetic composition.
- A use of glacier water for relieving roughness of skin surface in a cosmetic composition.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2008-0004325 | 2008-01-15 | ||
| KR1020080004325A KR100963093B1 (en) | 2008-01-15 | 2008-01-15 | Method for extracting using Himalayan Glacial Water and the extracts thereof, and cosmetic composition containing nanoparticles encapsulated the extracts |
| KR1020080062383A KR101199135B1 (en) | 2008-06-30 | 2008-06-30 | Cosmetic composition containing Himalayan Glacial Water |
| KR10-2008-0062383 | 2008-06-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2009091183A2 true WO2009091183A2 (en) | 2009-07-23 |
| WO2009091183A3 WO2009091183A3 (en) | 2009-10-22 |
Family
ID=40885796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/KR2009/000200 WO2009091183A2 (en) | 2008-01-15 | 2009-01-15 | Method for extracting using glacial water and the extract thereof, and cosmetic composition comprising namoemulsion particles having the extract encapsulated or the glacial water. |
Country Status (2)
| Country | Link |
|---|---|
| TW (1) | TWI477291B (en) |
| WO (1) | WO2009091183A2 (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120020903A1 (en) * | 2010-07-21 | 2012-01-26 | Lvmh Recherche | Cosmetic composition containing a particular water, and use thereof as a depigmenting or anti-ageing agent |
| US20130287714A1 (en) * | 2010-11-12 | 2013-10-31 | Sven Gohla | Cosmetic and/or dermatological preparations containing snow algae extract |
| CN104610203A (en) * | 2015-03-05 | 2015-05-13 | 国家林业局泡桐研究开发中心 | Method for extracting vitamin C from shine skin papayas |
| CN105399780A (en) * | 2015-12-08 | 2016-03-16 | 黄涌芮 | Method for extracting vitamins from mangoes |
| CN105418692A (en) * | 2015-12-08 | 2016-03-23 | 黄涌芮 | Method for extracting vitamins from apple |
| CN105503966A (en) * | 2015-12-08 | 2016-04-20 | 黄涌芮 | Method for extracting vitamin from cucumber |
| CN109125163A (en) * | 2017-08-25 | 2019-01-04 | 伽蓝（集团）股份有限公司 | Glacial water composite extract of rough gentian, emblic and Flos meconopsis quintuplinerviae and its preparation method and application |
| CN110731917A (en) * | 2018-07-20 | 2020-01-31 | 伽蓝（集团）股份有限公司 | preparation containing glacier water, and its preparation method and application |
| CN113862176A (en) * | 2021-09-04 | 2021-12-31 | 赣州澳丽尔化妆品有限公司 | Preparation method of aureobasidium pullulans |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105497089B (en) * | 2014-09-25 | 2020-10-23 | 伽蓝（集团）股份有限公司 | Composition containing coreopsis tinctoria extract, application thereof and skin external preparation |
| CN108653064B (en) * | 2017-03-27 | 2022-06-10 | 伽蓝（集团）股份有限公司 | Snow tea extract and preparation method and application thereof |
| CN109090394A (en) * | 2018-08-13 | 2018-12-28 | 安徽国科生物科技有限公司 | A kind of preparation method preventing and treating four high low deuterium-oxide beverages |
| CN109134696A (en) * | 2018-08-13 | 2019-01-04 | 安徽国科生物科技有限公司 | The method that a kind of low deuterium-oxide of rich trace elements extracts small molecule mushroom polysaccharide |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100394770B1 (en) * | 2001-06-05 | 2003-08-14 | 주식회사 태평양 | Stabilization method of nano-emulsion using tocopheryl derivatives and external application for skin containing the stabilized nano-emulsion |
| JP2005213416A (en) * | 2004-01-30 | 2005-08-11 | Pixen Inc | Perfume |
| KR20070020340A (en) * | 2005-08-15 | 2007-02-21 | 오은영 | Natural compositions and functional drinking water prepared by including natural extracts and functional water as components |
| KR100778811B1 (en) * | 2006-02-28 | 2007-11-28 | 주식회사 워터비스 | Method for extracting functional materials of plants using an extracting solvent comprising deep sea water |
-
2009
- 2009-01-14 TW TW098101191A patent/TWI477291B/en active
- 2009-01-15 WO PCT/KR2009/000200 patent/WO2009091183A2/en active Application Filing
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120020903A1 (en) * | 2010-07-21 | 2012-01-26 | Lvmh Recherche | Cosmetic composition containing a particular water, and use thereof as a depigmenting or anti-ageing agent |
| US9700496B2 (en) | 2010-07-21 | 2017-07-11 | Lvmh Recherche | Cosmetic composition containing a particular water, and use thereof as a depigmenting or anti-ageing agent |
| US20130287714A1 (en) * | 2010-11-12 | 2013-10-31 | Sven Gohla | Cosmetic and/or dermatological preparations containing snow algae extract |
| CN104610203A (en) * | 2015-03-05 | 2015-05-13 | 国家林业局泡桐研究开发中心 | Method for extracting vitamin C from shine skin papayas |
| CN105399780A (en) * | 2015-12-08 | 2016-03-16 | 黄涌芮 | Method for extracting vitamins from mangoes |
| CN105418692A (en) * | 2015-12-08 | 2016-03-23 | 黄涌芮 | Method for extracting vitamins from apple |
| CN105503966A (en) * | 2015-12-08 | 2016-04-20 | 黄涌芮 | Method for extracting vitamin from cucumber |
| CN109125163A (en) * | 2017-08-25 | 2019-01-04 | 伽蓝（集团）股份有限公司 | Glacial water composite extract of rough gentian, emblic and Flos meconopsis quintuplinerviae and its preparation method and application |
| CN109125163B (en) * | 2017-08-25 | 2022-07-22 | 伽蓝（集团）股份有限公司 | Glacier water composite extract of gentian, emblic leafflower fruit and artemisia integrifolia as well as preparation method and application thereof |
| CN110731917A (en) * | 2018-07-20 | 2020-01-31 | 伽蓝（集团）股份有限公司 | preparation containing glacier water, and its preparation method and application |
| CN113862176A (en) * | 2021-09-04 | 2021-12-31 | 赣州澳丽尔化妆品有限公司 | Preparation method of aureobasidium pullulans |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009091183A3 (en) | 2009-10-22 |
| TWI477291B (en) | 2015-03-21 |
| TW200936177A (en) | 2009-09-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2009091183A2 (en) | Method for extracting using glacial water and the extract thereof, and cosmetic composition comprising namoemulsion particles having the extract encapsulated or the glacial water. | |
| CN108904302A (en) | A kind of fullerene topical composition | |
| WO2013027984A2 (en) | Cosmetic composition containing green tea component | |
| WO2011122869A2 (en) | Cosmetic composition comprising coumestrol or a bean extract containing coumestrol for skin care | |
| KR101526266B1 (en) | Extraction method using deep sea water and the extracts thereby, and the cosmetic composition containing nanoparticles encapsulated the extracts | |
| KR20110059986A (en) | Water in oil type make-up cosmetic composition for improving skin | |
| KR102236253B1 (en) | Cosmetic composition for skin improvement comprising extracts derived from natural products and method for manufacturing the same | |
| WO2012070804A2 (en) | Cosmetic composition containing an oleanolic acid | |
| WO2010064878A2 (en) | Anti-wrinkle cosmetic skin composition containing leontopodic acid as an active ingredient | |
| KR101908977B1 (en) | A cosmetic composition for pore-minimizing and sebum control comprising lens esculenta, ceratonia siliqua, nelumbo nucifera and chenopodium quinoa extract | |
| KR100824212B1 (en) | Composition for skin protection containing placenta and preparation method thereof | |
| KR100994987B1 (en) | Cosmetic composition with the calming effect for skins | |
| KR102092177B1 (en) | A cosmetic compositon for anti-pollution containing extracts of brassica oleracea | |
| KR100896697B1 (en) | Percutaneous absorption enhancing method of Rheum coreanum by high pressure-emulsion technology, and skin care and pharmaceutical compositions with whitening effect for external applications utilizing thereof | |
| KR20100006796A (en) | Anti-aging cosmetic composition comprising herb ferment extract | |
| KR101862039B1 (en) | A cosmetic composition containing spicule powder and natural complex extract stabilized in nanoliposome | |
| WO2023085578A1 (en) | Method for preparing fermented vegetable oil by means of skin flora, and cosmetic composition comprising same | |
| WO2010004099A2 (en) | Skin-regenerating composition | |
| KR100963093B1 (en) | Method for extracting using Himalayan Glacial Water and the extracts thereof, and cosmetic composition containing nanoparticles encapsulated the extracts | |
| CN115813774A (en) | Water-oil double-layer refined composition with whitening effect and preparation method and application thereof | |
| KR102424510B1 (en) | cosmetic composition for antiirritation and skin soothing using Lactobacillus rhamnosus J2K-187strain | |
| KR102247589B1 (en) | Cosmetic composition for improving skin comprising the moluccella laevis extraction and the hololeion maximowiczii Kitam. extraction as an effective ingredient | |
| WO2021049742A1 (en) | Cosmetic composition having excellent percutaneous absorption | |
| KR101969684B1 (en) | A cosmetic composition for anti-wrinkle and skin pore reduction containing natural spicule powder and natural complex extract | |
| KR101214344B1 (en) | Cosmetics composition containing fermented rice bran and licorice extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 09701668 Country of ref document: EP Kind code of ref document: A2 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 09701668 Country of ref document: EP Kind code of ref document: A2 |